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Targeting the TRAF3-ULK1-NLRP3 regulatory axis to control alveolar macrophage pyroptosis in acute lung injury
This study further reveal that the ULK1 protein diminishes the expression of NLRP3, thereby reducing the immune response of alveolar macrophages and mitigating ALI. Conversely, TRAF3, another protein, is found to inhibit ULK1 through a process called ubiquitination, leading to increased activation of the NLRP3 inflammasome and exacerbation of ALI. This TRAF3-mediated suppression of ULK1 and subsequent activation of NLRP3 are confirmed through various in vitro and in vivo experiments. The presence of abundant M0 and M1 alveolar macrophages in the ALI tissue samples further support these findings. This research highlights th...
Source: Acta Biochimica et Biophysica Sinica - April 30, 2024 Category: Biochemistry Authors: Lei Jiang Chunlin Ye Yunhe Huang Zhi Hu Guangxia Wei Source Type: research

circIARS: a potential plasma biomarker for diagnosing non-small cell lung cancer
Acta Biochim Biophys Sin (Shanghai). 2024 Apr 29. doi: 10.3724/abbs.2024043. Online ahead of print.ABSTRACTNon-small cell lung cancer (NSCLC) is one of the most prevalent cancers in the world, and early diagnosis can effectively improve patient survival. Here, differentially expressed circIARS genes are screened from the sequencing results, and their molecular characteristics are examined by Sanger sequencing, RNase R assay, agarose gel electrophoresis (AGE), and fluorescence in situ hybridization (FISH). Real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) is performed to detect the expression level of ...
Source: Acta Biochimica et Biophysica Sinica - April 30, 2024 Category: Biochemistry Authors: Qi Zhang Xinfeng Fan Xinyu Zhang Shaoqing Ju Source Type: research

Unraveling the metabolic potential of biocontrol fungi through omics data: a key to enhancing large-scaleapplication strategies
This article analyzes the preferences for extracellular carbon and nitrogen source degradation, secondary metabolites (nonribosomal peptides, polyketide synthases) and their product characteristics and the conversion relationship between extracellular primary metabolism and intracellular secondary metabolism for eight different filamentous fungi with characteristics appropriate for the biological control of bacterial pathogens and phytopathogenic nematodes. Further clarification is provided that Paecilomyces lilacinus, encoding a large number of hydrolase enzymes capable of degrading pathogen protection barrier, can be dir...
Source: Acta Biochimica et Biophysica Sinica - April 30, 2024 Category: Biochemistry Authors: Haolin Yang Xiuyun Wu Caiyun Sun Lushan Wang Source Type: research

Construction of a cell-based aggregation and seeding model for the Tau protein
Acta Biochim Biophys Sin (Shanghai). 2024 Apr 29. doi: 10.3724/abbs.2024057. Online ahead of print.NO ABSTRACTPMID:38682159 | DOI:10.3724/abbs.2024057 (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - April 29, 2024 Category: Biochemistry Authors: Jiying Hu Liqiang Wang Jie Chen Yi Liang Source Type: research

FANCA facilitates G1/S cell cycle advancement, proliferation, migration and invasion in gastric cancer
Acta Biochim Biophys Sin (Shanghai). 2024 Apr 29. doi: 10.3724/abbs.2024045. Online ahead of print.ABSTRACTThe present study explores the function of FANCA gene, a pivotal member of the Fanconi anaemia (FA) pathway crucial for preserving genomic stability and preventing cancer, particularly in the context of gastric cancer (GC). Using immunohistochemistry, quantitative real-time PCR, and western blot analysis, we evaluate FANCA mRNA and protein expressions in GC cell lines. The relationship between FANCA expression and clinicopathological characteristics is also explored. Various assays, including CCK8, colony formation, w...
Source: Acta Biochimica et Biophysica Sinica - April 29, 2024 Category: Biochemistry Authors: Wei Wang Shantanu Baral Bin Liu Qiannan Sun Liuhua Wang Jun Ren Dong Tang Daorong Wang Source Type: research

miR-194-3p regulates epithelial-mesenchymal transition in embryonic epicardial cells via p120/ β-catenin signaling
In conclusion, miR-194-3p directly targets p120-catenin and modulates its expression, which in turn alters β-catenin expression, critically influencing the EMT process in the embryonic epicardial cells via the cell adhesion mechanism.PMID:38676398 | DOI:10.3724/abbs.2024051 (Source: Acta Biochimica et Biophysica Sinica)
Source: Acta Biochimica et Biophysica Sinica - April 27, 2024 Category: Biochemistry Authors: Tianhua Xiong Dinghui Wang Huiping Yang Bin Liu Yingrui Li Wenlong Yu Jing Wang Qiang She Source Type: research

Molecular stratification of the human fetal vaginal epithelium by spatial transcriptome analysis
In this study, we perform a spatial transcriptomic analysis within the vaginal wall of human fetuses to fill this knowledge gap. We successfully categorize the vaginal epithelium into four distinct zones based on transcriptomic profiles and anatomical features. This approach reveals unique transcriptomic signatures within these regions, allowing us to identify differentially expressed genes and uncover novel markers for distinct regions of the vaginal epithelium. Additionally, our findings highlight the varied expressions of keratin ( KRT) genes across different zones of the vaginal epithelium, with a gradual shift in expr...
Source: Acta Biochimica et Biophysica Sinica - April 26, 2024 Category: Biochemistry Authors: Ziying Ye Peipei Jiang Qi Zhu Zhongrui Pei Yali Hu Guangfeng Zhao Source Type: research

Magnolol promotes the autophagy of esophageal carcinoma cells by upregulating < em > HACE1 < /em > gene expression
This study demonstrates that magnolol increases the protein levels of LC3 II, accompanied by increased HACE1 protein levels in both esophageal carcinoma cells and xenograft tumors. HACE1-knockout (KO) cell lines are generated, and the ablation of HACE1 eliminates the anti-proliferative and autophagy-inducing effects of magnolol on esophageal carcinoma cells. Additionally, our results show that magnolol primarily promotes HACE1 expression at the transcriptional level. Therefore, this study shows that magnolol primarily exerts its antitumor effect by activating HACE1-OPTN axis-mediated autophagy. It can be considered a promi...
Source: Acta Biochimica et Biophysica Sinica - April 25, 2024 Category: Biochemistry Authors: Kenan Huang Biao Zhang Yu Feng Haitao Ma Source Type: research

Disruption of a DNA G-quadruplex causes a gain-of-function < em > SCL45A1 < /em > variant relevant to developmental disorders
In this study, we identify a functional DNA G-quadruplex and its key binding site on SLC45A1 (NM_001080397.3: exon 2: c.449 G>A: p.R150K). This variant results in the upregulation of mRNA and protein expression, which may lead to intellectual developmental disorder with neuropsychiatric features. Mechanistically, the mutation is found to disrupt DNA G-quadruplex structures on SLC45A1, leading to transcriptional enhancement and a gain-of-function mutation, which further causes increased expression and function of the SLC45A1 protein. The identification of the functional DNA G-quadruplex and its effects on DNA G4s may pro...
Source: Acta Biochimica et Biophysica Sinica - April 24, 2024 Category: Biochemistry Authors: Yuxi Chen Jiang Long Sixian Wu Yazhen Wei Fei Yan Qing Li Jierui Yan Nannan Zhang Wenming Xu Source Type: research

Construction and efficacy testing of DNA vaccines containing HLA-A*02:01-restricted SARS-CoV-2 T-cell epitopes predicted by immunoinformatics
Acta Biochim Biophys Sin (Shanghai). 2024 Apr 24. doi: 10.3724/abbs.2024039. Online ahead of print.ABSTRACTVaccines play essential roles in the fight against the COVID-19 pandemic. The development and assessment of COVID-19 vaccines have generally focused on the induction and boosting of neutralizing antibodies targeting the SARS-CoV-2 spike (S) protein. Due to rapid and continuous variation in the S protein, such vaccines need to be regularly updated to match newly emerged dominant variants. T-cell vaccines that target MHC I- or II-restricted epitopes in both structural and non-structural viral proteins have the potential...
Source: Acta Biochimica et Biophysica Sinica - April 24, 2024 Category: Biochemistry Authors: Dan Tan Ning Kang Yuanfei Zhu Jia Hou Hanqing Wang Huijun Xu Cheng Zu Zixiang Gao Mu Liu Nannan Liu Qiang Deng Hongzhou Lu Jing Liu Youhua Xie Source Type: research

Bronchial thermoplasty decreases airway remodeling by inhibiting autophagy via the AMPK/mTOR signaling pathway
In this study, we aim to elucidate the autophagy-mediated molecular mechanisms in BT. Our study reveal that the number of autophagosomes and the level of alpha-smooth muscle actin (α-SMA) fluorescence are significantly decreased in airway biopsy tissues after BT. As the temperature increased, BT causes a decrease in cell proliferation and a concomitant increase in the apoptosis of human airway smooth muscle cells (HASMCs). Furthermore, increase in temperature significantly downregulates cellular autophagy, autophagosome accumulation, the LC3II/LC3I ratio, and Beclin-1 expression, upregulates p62 expression, and inhibits t...
Source: Acta Biochimica et Biophysica Sinica - April 24, 2024 Category: Biochemistry Authors: Tao Wang Peng Fu Wenting Huang Liang Long Fa Long Shengming Liu Source Type: research

AlphaFold2 assists in providing novel mechanistic insights into the interactions among the LUBAC subunits
Acta Biochim Biophys Sin (Shanghai). 2024 Apr 24. doi: 10.3724/abbs.2024047. Online ahead of print.ABSTRACTThe linear ubiquitin chain assembly complex (LUBAC) is the only known E3 ligase complex in which the ubiquitin-like (UBL) domains of SHARPIN and HOIL-1L interact with HOIP to determine the structural stability of LUBAC. The interactions between subunits within LUBAC have been a topic of extensive research. However, the impact of the LTM motif on the interaction between the UBL domains of SHARPIN and HOIL-1L with HOIP remains unclear. Here, we discover that the absence of the LTM motif in the AlphaFold2-predicted LUBAC...
Source: Acta Biochimica et Biophysica Sinica - April 24, 2024 Category: Biochemistry Authors: Chenchen Wang Chunying Gu Ying Lv Hongyu Liu Yanan Wang Yongmei Zuo Guangyu Jiang Lili Liu Jiafu Liu Source Type: research