Brachyury Promotes Extracellular Matrix Synthesis through Transcriptional Regulation of Smad3 in Nucleus Pulposus
In conclusion, this study reveals that Bry positively regulates the synthesis of collagen II and aggrecan in NP through transcriptional activation of Smad3.PMID:38690917 | DOI:10.1152/ajpcell.00475.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - May 1, 2024 Category: Cytology Authors: Yanzhang Xia Yinghui Wu Yuhao Gong Caichun Yue Linfeng Tao Tianwen Xin Cong Shen Yue Zhu Minghong Shen Jun Shen Source Type: research
IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype
Am J Physiol Cell Physiol. 2024 Mar 4. doi: 10.1152/ajpcell.00654.2023. Online ahead of print.ABSTRACTSkeletal muscle mediates the beneficial effects of exercise, thereby improving insulin sensitivity and reducing the risk for type 2 diabetes. Current human skeletal muscle models in vitro are incapable of fully recapitulating its physiological functions especially muscle contractility. By supplementation of insulin-like growth factor 1 (IGF1), a growth factor secreted by myofibers in vivo, we aimed to overcome these limitations. We monitored the differentiation process starting from primary human CD56-positive myoblasts in...
Source: Am J Physiol Cell Ph... - May 1, 2024 Category: Cytology Authors: Simon I Dreher Paul Grubba Christine von Toerne Alessia Moruzzi Jennifer Maurer Thomas Goj Andreas L Birkenfeld Andreas Peter Peter Loskill Stefanie M Hauck Cora Weigert Source Type: research
Brachyury Promotes Extracellular Matrix Synthesis through Transcriptional Regulation of Smad3 in Nucleus Pulposus
In conclusion, this study reveals that Bry positively regulates the synthesis of collagen II and aggrecan in NP through transcriptional activation of Smad3.PMID:38690917 | DOI:10.1152/ajpcell.00475.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - May 1, 2024 Category: Cytology Authors: Yanzhang Xia Yinghui Wu Yuhao Gong Caichun Yue Linfeng Tao Tianwen Xin Cong Shen Yue Zhu Minghong Shen Jun Shen Source Type: research
IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype
Am J Physiol Cell Physiol. 2024 Mar 4. doi: 10.1152/ajpcell.00654.2023. Online ahead of print.ABSTRACTSkeletal muscle mediates the beneficial effects of exercise, thereby improving insulin sensitivity and reducing the risk for type 2 diabetes. Current human skeletal muscle models in vitro are incapable of fully recapitulating its physiological functions especially muscle contractility. By supplementation of insulin-like growth factor 1 (IGF1), a growth factor secreted by myofibers in vivo, we aimed to overcome these limitations. We monitored the differentiation process starting from primary human CD56-positive myoblasts in...
Source: Am J Physiol Cell Ph... - May 1, 2024 Category: Cytology Authors: Simon I Dreher Paul Grubba Christine von Toerne Alessia Moruzzi Jennifer Maurer Thomas Goj Andreas L Birkenfeld Andreas Peter Peter Loskill Stefanie M Hauck Cora Weigert Source Type: research
Brachyury Promotes Extracellular Matrix Synthesis through Transcriptional Regulation of Smad3 in Nucleus Pulposus
In conclusion, this study reveals that Bry positively regulates the synthesis of collagen II and aggrecan in NP through transcriptional activation of Smad3.PMID:38690917 | DOI:10.1152/ajpcell.00475.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - May 1, 2024 Category: Cytology Authors: Yanzhang Xia Yinghui Wu Yuhao Gong Caichun Yue Linfeng Tao Tianwen Xin Cong Shen Yue Zhu Minghong Shen Jun Shen Source Type: research
IGF1 promotes human myotube differentiation toward a mature metabolic and contractile phenotype
Am J Physiol Cell Physiol. 2024 Mar 4. doi: 10.1152/ajpcell.00654.2023. Online ahead of print.ABSTRACTSkeletal muscle mediates the beneficial effects of exercise, thereby improving insulin sensitivity and reducing the risk for type 2 diabetes. Current human skeletal muscle models in vitro are incapable of fully recapitulating its physiological functions especially muscle contractility. By supplementation of insulin-like growth factor 1 (IGF1), a growth factor secreted by myofibers in vivo, we aimed to overcome these limitations. We monitored the differentiation process starting from primary human CD56-positive myoblasts in...
Source: Am J Physiol Cell Ph... - May 1, 2024 Category: Cytology Authors: Simon I Dreher Paul Grubba Christine von Toerne Alessia Moruzzi Jennifer Maurer Thomas Goj Andreas L Birkenfeld Andreas Peter Peter Loskill Stefanie M Hauck Cora Weigert Source Type: research
Hemoglobin scavenger receptor CD163 as a potential biomarker of hemolysis induced hepatobiliary injury in sickle cell disease
Am J Physiol Cell Physiol. 2024 Apr 29. doi: 10.1152/ajpcell.00386.2023. Online ahead of print.ABSTRACTSickle cell disease (SCD) associated chronic hemolysis promotes oxidative stress, inflammation and thrombosis leading to organ damage, including liver damage. Hemoglobin scavenger receptor CD163 plays a protective role in SCD by scavenging both hemoglobin-haptoglobin complexes and cell free hemoglobin. A limited number of studies in the past have shown a positive correlation of CD163 expression with poor disease outcomes in patients with SCD. However, the role and regulation of CD163 in SCD related hepatobiliary injury ha...
Source: Am J Physiol Cell Ph... - April 29, 2024 Category: Cytology Authors: Tomasz W Kaminski Ayynar Sivanantham Anna Mozhenkova Ashley Smith Ramakrishna Ungalara Rikesh Dubey Bibhav Shrestha Corinne Hanway Omika Katoch Jes ús Tejero Prithu Sundd Enrico M Novelli Gregory J Kato Tirthadipa Pradhan-Sundd Source Type: research
Metformin inhibits nerve growth factor (NGF)-induced sympathetic neuron differentiation through p35/CDK5 inhibition
In this study, we investigated the impact of metformin on NGF-induced sympathetic neuronal differentiation using rat pheochromocytoma PC12 cells as a model. We examined the regulation of TrkA-p35/CDK5 signaling in NGF-induced PC12 differentiation. Our results demonstrate that metformin reduces NGF-induced PC12 differentiation by inactivating the TrkA receptor, subsequently inhibiting ERK and EGR1. Inhibition of this cascade ultimately leads to the down-regulation of p35/CDK5 in PC12 cells. Furthermore, metformin inhibits the activation of the pre-synaptic protein Synapsin-I, a substrate of CDK5, in PC12 differentiation. Ad...
Source: Am J Physiol Cell Ph... - April 29, 2024 Category: Cytology Authors: Muhammet Oner Mei-Chih Chen Pang-Ting Cheng Ho Lin Source Type: research
Acute effects of a ketone monoester, whey protein, or their co-ingestion on mTOR trafficking and protein-protein co-localization in human skeletal muscle
Am J Physiol Cell Physiol. 2024 Apr 29. doi: 10.1152/ajpcell.00207.2024. Online ahead of print.ABSTRACTWe recently demonstrated that acute oral ketone monoester intake induces a stimulation of postprandial myofibrillar protein synthesis rates comparable to that elicited following the ingestion of 10 g whey protein or their co-ingestion. The present investigation aimed to determine the acute effects of ingesting a ketone monoester, whey protein, or their co-ingestion on mTOR-related protein-protein co-localization and intracellular trafficking in human skeletal muscle. In a randomized, double-blind, parallel group design, 3...
Source: Am J Physiol Cell Ph... - April 29, 2024 Category: Cytology Authors: Sarkis J Hannaian Jamie Lov Zacharie Cheng-Boivin Sidney Abou Sawan Nathan Hodson Benoit J Gentil Jos é A Morais Tyler A Churchward-Venne Source Type: research
Mogrol-Mediated enhancement of radiotherapy sensitivity in Non-Small cell lung cancer: A mechanistic study
CONCLUSION: Mogrol enhances NSCLC radiosensitivity by downregulating USP22 via the ERK/CREB pathway, leading to reduced COX2 expression.PMID:38682239 | DOI:10.1152/ajpcell.00684.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - April 29, 2024 Category: Cytology Authors: Zhongbo Yin Xuedong Zhang Xiao Sun Yunlong Huo Nan Ji Keyan Chen Source Type: research
A new mechanism in negative pressure wound therapy: interleukin-17 alters chromatin accessibility profiling
In this study, skin wound-healing models were established, with administration of NPWT. Vimentin, collagen I, and MMP9 of skin tissues were detected by immunofluorescence (IF). Gene expression analysis of skin wound tissues was performed by RNA-sequencing (RNA-seq). Protein expression was assayed by a western blotting or IF assay, and mRNA levels were quantified by quantitative PCR. Chromatin accessibility profiles of fibroblasts following NPWT or IL-17 exposure were analyzed by ATAC-seq. In rat wound-healing models, NPWT promoted wound repair by promoting re-epithelialization, extracellular matrix (ECM) synthesis and prol...
Source: Am J Physiol Cell Ph... - April 29, 2024 Category: Cytology Authors: Shuao Xiao Wenxuan Wang Congying Zhao Pan Ren Liwei Dong Hao Zhang Fuxin Ma Xueyong Li Yongqian Bian Source Type: research
Hemoglobin scavenger receptor CD163 as a potential biomarker of hemolysis induced hepatobiliary injury in sickle cell disease
Am J Physiol Cell Physiol. 2024 Apr 29. doi: 10.1152/ajpcell.00386.2023. Online ahead of print.ABSTRACTSickle cell disease (SCD) associated chronic hemolysis promotes oxidative stress, inflammation and thrombosis leading to organ damage, including liver damage. Hemoglobin scavenger receptor CD163 plays a protective role in SCD by scavenging both hemoglobin-haptoglobin complexes and cell free hemoglobin. A limited number of studies in the past have shown a positive correlation of CD163 expression with poor disease outcomes in patients with SCD. However, the role and regulation of CD163 in SCD related hepatobiliary injury ha...
Source: Am J Physiol Cell Ph... - April 29, 2024 Category: Cytology Authors: Tomasz W Kaminski Ayynar Sivanantham Anna Mozhenkova Ashley Smith Ramakrishna Ungalara Rikesh Dubey Bibhav Shrestha Corinne Hanway Omika Katoch Jes ús Tejero Prithu Sundd Enrico M Novelli Gregory J Kato Tirthadipa Pradhan-Sundd Source Type: research
Metformin inhibits nerve growth factor (NGF)-induced sympathetic neuron differentiation through p35/CDK5 inhibition
In this study, we investigated the impact of metformin on NGF-induced sympathetic neuronal differentiation using rat pheochromocytoma PC12 cells as a model. We examined the regulation of TrkA-p35/CDK5 signaling in NGF-induced PC12 differentiation. Our results demonstrate that metformin reduces NGF-induced PC12 differentiation by inactivating the TrkA receptor, subsequently inhibiting ERK and EGR1. Inhibition of this cascade ultimately leads to the down-regulation of p35/CDK5 in PC12 cells. Furthermore, metformin inhibits the activation of the pre-synaptic protein Synapsin-I, a substrate of CDK5, in PC12 differentiation. Ad...
Source: Am J Physiol Cell Ph... - April 29, 2024 Category: Cytology Authors: Muhammet Oner Mei-Chih Chen Pang-Ting Cheng Ho Lin Source Type: research
Acute effects of a ketone monoester, whey protein, or their co-ingestion on mTOR trafficking and protein-protein co-localization in human skeletal muscle
Am J Physiol Cell Physiol. 2024 Apr 29. doi: 10.1152/ajpcell.00207.2024. Online ahead of print.ABSTRACTWe recently demonstrated that acute oral ketone monoester intake induces a stimulation of postprandial myofibrillar protein synthesis rates comparable to that elicited following the ingestion of 10 g whey protein or their co-ingestion. The present investigation aimed to determine the acute effects of ingesting a ketone monoester, whey protein, or their co-ingestion on mTOR-related protein-protein co-localization and intracellular trafficking in human skeletal muscle. In a randomized, double-blind, parallel group design, 3...
Source: Am J Physiol Cell Ph... - April 29, 2024 Category: Cytology Authors: Sarkis J Hannaian Jamie Lov Zacharie Cheng-Boivin Sidney Abou Sawan Nathan Hodson Benoit J Gentil Jos é A Morais Tyler A Churchward-Venne Source Type: research
Mogrol-Mediated enhancement of radiotherapy sensitivity in Non-Small cell lung cancer: A mechanistic study
CONCLUSION: Mogrol enhances NSCLC radiosensitivity by downregulating USP22 via the ERK/CREB pathway, leading to reduced COX2 expression.PMID:38682239 | DOI:10.1152/ajpcell.00684.2023 (Source: Am J Physiol Cell Ph...)
Source: Am J Physiol Cell Ph... - April 29, 2024 Category: Cytology Authors: Zhongbo Yin Xuedong Zhang Xiao Sun Yunlong Huo Nan Ji Keyan Chen Source Type: research
