Theophylline-based control of repA on a Clostridioides difficile plasmid for use in allelic exchange
Anaerobe. 2024 Apr 29:102858. doi: 10.1016/j.anaerobe.2024.102858. Online ahead of print.ABSTRACTHistorically, mutagenesis in the non-model enteropathogenic bacterium Clostridioides difficile has been challenging. Developing a versatile and reliable method of generating targeted mutations in C. difficile is important to further our understanding of its pathogenesis. Some of the most common targeted mutagenesis systems rely on allelic exchange mediated by either uracil auxotrophy combined with a toxic uracil precursor, a toxin / anti-toxin system, group II introns, or CRISPR/Cas mutagenesis. However, each of these methods s...
Source: Anaerobe - May 1, 2024 Category: Microbiology Authors: Joshua N Brehm Joseph A Sorg Source Type: research
Genotypic and phenotypic antimicrobial resistance of Irish Clostridioides difficile isolates, 2022
CONCLUSION: Our results suggest that for moxifloxacin, tetracycline and clindamycin, phenotypic resistance in C. difficile can be predicted by genetic indicators and used for public health purposes. However, for the other five antibiotics, the model is not accurate and further development is necessary.PMID:38670525 | DOI:10.1016/j.anaerobe.2024.102857 (Source: Anaerobe)
Source: Anaerobe - April 26, 2024 Category: Microbiology Authors: Lieke Brouwer Anne Carroll Eleanor McNamara Source Type: research
Genotypic and phenotypic antimicrobial resistance of Irish Clostridioides difficile isolates, 2022
CONCLUSION: Our results suggest that for moxifloxacin, tetracycline and clindamycin, phenotypic resistance in C. difficile can be predicted by genetic indicators and used for public health purposes. However, for the other five antibiotics, the model is not accurate and further development is necessary.PMID:38670525 | DOI:10.1016/j.anaerobe.2024.102857 (Source: Anaerobe)
Source: Anaerobe - April 26, 2024 Category: Microbiology Authors: Lieke Brouwer Anne Carroll Eleanor McNamara Source Type: research
Genotypic and phenotypic antimicrobial resistance of Irish Clostridioides difficile isolates, 2022
CONCLUSION: Our results suggest that for moxifloxacin, tetracycline and clindamycin, phenotypic resistance in C. difficile can be predicted by genetic indicators and used for public health purposes. However, for the other five antibiotics, the model is not accurate and further development is necessary.PMID:38670525 | DOI:10.1016/j.anaerobe.2024.102857 (Source: Anaerobe)
Source: Anaerobe - April 26, 2024 Category: Microbiology Authors: Lieke Brouwer Anne Carroll Eleanor McNamara Source Type: research
Genotypic and phenotypic antimicrobial resistance of Irish Clostridioides difficile isolates, 2022
CONCLUSION: Our results suggest that for moxifloxacin, tetracycline and clindamycin, phenotypic resistance in C. difficile can be predicted by genetic indicators and used for public health purposes. However, for the other five antibiotics, the model is not accurate and further development is necessary.PMID:38670525 | DOI:10.1016/j.anaerobe.2024.102857 (Source: Anaerobe)
Source: Anaerobe - April 26, 2024 Category: Microbiology Authors: Lieke Brouwer Anne Carroll Eleanor McNamara Source Type: research
Genotypic and phenotypic antimicrobial resistance of Irish Clostridioides difficile isolates, 2022
CONCLUSION: Our results suggest that for moxifloxacin, tetracycline and clindamycin, phenotypic resistance in C. difficile can be predicted by genetic indicators and used for public health purposes. However, for the other five antibiotics, the model is not accurate and further development is necessary.PMID:38670525 | DOI:10.1016/j.anaerobe.2024.102857 (Source: Anaerobe)
Source: Anaerobe - April 26, 2024 Category: Microbiology Authors: Lieke Brouwer Anne Carroll Eleanor McNamara Source Type: research
Exploring the effect of Clostridium butyricum on lung injury associated with acute pancreatitis in mice by combined 16S rRNA and metabolomics analysis
CONCLUSIONS: C. butyricum may alleviate lung injury associated with AP by interfering with the relevant intestinal microbiota and modulating relevant metabolic pathways.PMID:38614288 | DOI:10.1016/j.anaerobe.2024.102854 (Source: Anaerobe)
Source: Anaerobe - April 13, 2024 Category: Microbiology Authors: Jiaxin Liu Biyan Wen Yaoxing Huang Guiqing Deng Qingqing Yan Lin Jia Source Type: research
Optimization of CO fermentation by Clostridium carboxidivorans in batch reactors: Effects of the medium composition
CONCLUSIONS: The optimization of the medium composition in CO fermentation by Clostridium carboxidivorans is crucial for cost-effective solvent production. Tuning the yeast extract (YE) concentration, using pure cysteine as the reducing agent and reducing trace metal concentration contribute to reasonable solvent production within a relatively short fermentation period. Tungsten is essential for cell growth and solvent production, while selenium is not required.PMID:38614289 | DOI:10.1016/j.anaerobe.2024.102855 (Source: Anaerobe)
Source: Anaerobe - April 13, 2024 Category: Microbiology Authors: F Lanzillo S Pisacane F Raganati M E Russo P Salatino A Marzocchella Source Type: research
Human fecal alpha-glucosidase activity and its relationship with gut microbiota profiles and early stages of intestinal mucosa damage
CONCLUSIONS: Our findings suggest a relationship among altered fecal α-glucosidase levels, the presence of intestinal mucosal lesions, which can be precursors of CRC, and shifts in defined microbial groups of the fecal microbiota.PMID:38614290 | DOI:10.1016/j.anaerobe.2024.102853 (Source: Anaerobe)
Source: Anaerobe - April 13, 2024 Category: Microbiology Authors: Sergio Ruiz-Saavedra Nuria Salazar Adolfo Su árez Ylenia Diaz Carmen Gonz ález Del Rey Sonia Gonz ález Clara G de Los Reyes-Gavil án Source Type: research
Novel transcriptional regulator OxtR1 regulates potential ferrodoxin in response to oxygen stress in Treponema denticola
CONCLUSION: The oxygen-sensing regulator OxtR1 plays a role in regulating the expression of a potential ferrodoxin, which may contribute to the response of T. denticola to oxygen-induced stress.PMID:38614291 | DOI:10.1016/j.anaerobe.2024.102852 (Source: Anaerobe)
Source: Anaerobe - April 13, 2024 Category: Microbiology Authors: Yumi Numata Yuichiro Kikuchi Toru Sato Kazuko Okamoto-Shibayama Yutaro Ando Yuri Miyai-Murai Eitoyo Kokubu Kazuyuki Ishihara Source Type: research
Exploring the effect of Clostridium butyricum on lung injury associated with acute pancreatitis in mice by combined 16S rRNA and metabolomics analysis
CONCLUSIONS: C. butyricum may alleviate lung injury associated with AP by interfering with the relevant intestinal microbiota and modulating relevant metabolic pathways.PMID:38614288 | DOI:10.1016/j.anaerobe.2024.102854 (Source: Anaerobe)
Source: Anaerobe - April 13, 2024 Category: Microbiology Authors: Jiaxin Liu Biyan Wen Yaoxing Huang Guiqing Deng Qingqing Yan Lin Jia Source Type: research
Optimization of CO fermentation by Clostridium carboxidivorans in batch reactors: Effects of the medium composition
CONCLUSIONS: The optimization of the medium composition in CO fermentation by Clostridium carboxidivorans is crucial for cost-effective solvent production. Tuning the yeast extract (YE) concentration, using pure cysteine as the reducing agent and reducing trace metal concentration contribute to reasonable solvent production within a relatively short fermentation period. Tungsten is essential for cell growth and solvent production, while selenium is not required.PMID:38614289 | DOI:10.1016/j.anaerobe.2024.102855 (Source: Anaerobe)
Source: Anaerobe - April 13, 2024 Category: Microbiology Authors: F Lanzillo S Pisacane F Raganati M E Russo P Salatino A Marzocchella Source Type: research
Human fecal alpha-glucosidase activity and its relationship with gut microbiota profiles and early stages of intestinal mucosa damage
CONCLUSIONS: Our findings suggest a relationship among altered fecal α-glucosidase levels, the presence of intestinal mucosal lesions, which can be precursors of CRC, and shifts in defined microbial groups of the fecal microbiota.PMID:38614290 | DOI:10.1016/j.anaerobe.2024.102853 (Source: Anaerobe)
Source: Anaerobe - April 13, 2024 Category: Microbiology Authors: Sergio Ruiz-Saavedra Nuria Salazar Adolfo Su árez Ylenia Diaz Carmen Gonz ález Del Rey Sonia Gonz ález Clara G de Los Reyes-Gavil án Source Type: research
Novel transcriptional regulator OxtR1 regulates potential ferrodoxin in response to oxygen stress in Treponema denticola
CONCLUSION: The oxygen-sensing regulator OxtR1 plays a role in regulating the expression of a potential ferrodoxin, which may contribute to the response of T. denticola to oxygen-induced stress.PMID:38614291 | DOI:10.1016/j.anaerobe.2024.102852 (Source: Anaerobe)
Source: Anaerobe - April 13, 2024 Category: Microbiology Authors: Yumi Numata Yuichiro Kikuchi Toru Sato Kazuko Okamoto-Shibayama Yutaro Ando Yuri Miyai-Murai Eitoyo Kokubu Kazuyuki Ishihara Source Type: research
Exploring the effect of Clostridium butyricum on lung injury associated with acute pancreatitis in mice by combined 16S rRNA and metabolomics analysis
CONCLUSIONS: C. butyricum may alleviate lung injury associated with AP by interfering with the relevant intestinal microbiota and modulating relevant metabolic pathways.PMID:38614288 | DOI:10.1016/j.anaerobe.2024.102854 (Source: Anaerobe)
Source: Anaerobe - April 13, 2024 Category: Microbiology Authors: Jiaxin Liu Biyan Wen Yaoxing Huang Guiqing Deng Qingqing Yan Lin Jia Source Type: research
