Liquid phase detection in the miniature scale. Microfluidic and capillary scale measurement and separation systems. A tutorial review
Anal Chim Acta. 2024 May 29;1305:342507. doi: 10.1016/j.aca.2024.342507. Epub 2024 Mar 23.ABSTRACTMicrofluidic and capillary devices are increasingly being used in analytical applications while their overall size keeps decreasing. Detection sensitivity for these microdevices gains more importance as device sizes and consequently, sample volumes, decrease. This paper reviews optical, electrochemical, electrical, and mass spectrometric detection methods that are applicable to capillary scale and microfluidic devices, with brief introduction to the principles in each case. Much of this is considered in the context of separati...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Cable G Warren Purnendu K Dasgupta Source Type: research
The sphingolipids change in exosomes from cancer patients and association between exosome release and sphingolipids level based on a pseudotargeted lipidomics method
Anal Chim Acta. 2024 May 29;1305:342527. doi: 10.1016/j.aca.2024.342527. Epub 2024 Mar 25.ABSTRACTThe lipid based ESCRT-independent mechanism, which contributes to MVB formation, is one of the crucial procedures in exosome biogenesis. n-SMase is a key lipid metabolism enzyme in this mechanism and can induce the hydrolysis of sphingomyelins (SMs) to ceramides (Cers), thereby promoting the formation of ILVs inside MVBs. Therefore, the regulation of n-SMase can realize the alteration in exosome release. According to the fact that cancer-associated cells have a tendency to release more exosomes than healthy cells, lipid extrac...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Kaige Yang Wenchang Fu Mengjiao Deng Xinyan Li Mingyuan Wu Yan Wang Source Type: research
Discovery of drug targets based on traditional Chinese medicine microspheres (TCM-MPs) fishing strategy combined with bio-layer interferometry (BLI) technology
In this study, a traditional Chinese medicine microspheres (TCM-MPs) target fishing strategy was developed to discover the key drug targets from complex system. The microspheres are composed of Fe3O4 magnetic nanolayer, oleic acid modified layer, the photoaffinity group (4- [3-(Trifluoromethyl)-3H-diazirin-3-yl] benzoic acid, TAD) layer and active small molecule layer from inside to outside. TAD produces highly reactive carbene under ultraviolet light, which can realize the self-assembly and fixation of drug active small molecules with non-selective properties. Here, taking Shenqi Jiangtang Granules (SJG) as an example, th...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Hui Zhang Jiangyu Yao Guyu Xiao Jianhui Xie Shuying Mao Chenghong Sun Jingchun Yao Jizhong Yan Pengfei Tu Source Type: research
Development of a novel fluorescent protein-based probe for efficient detection of Pb < sup > 2+ < /sup > in serum inspired by the metalloregulatory protein PbrR691
This study aims to fill this critical void by developing and evaluating a novel fluorescent protein-based probe that promises accurate and rapid lead quantification in blood.RESULTS: A simple and small-molecule fluorescent protein-based probe was successfully constructed herein using a peptide PbrBD designed for Pb2+ recognition coupled to a single fluorescent protein, sfGFP. The probe retains a three-coordinate configuration to identify Pb2+ and has a high affinity for it with a Kd' of 1.48 ± 0.05 × 10-17 M. It effectively transfers the conformational changes of the peptide to the chromophore upon Pb2+ binding, leading ...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Dan Wang Min Wei Liu Zhao Tianyu Song Qunfang Li Jiaxin Tan Jing Tang Zhipeng Li Rukui Zhu Source Type: research
Dual channel chemosensor for successive detection of environmentally toxic Pd < sup > 2+ < /sup > and CN < sup > - < /sup > ions and its application to cancer cell imaging
Anal Chim Acta. 2024 May 29;1305:342582. doi: 10.1016/j.aca.2024.342582. Epub 2024 Apr 10.ABSTRACTBACKGROUND: Detecting and neutralizing Pd2+ ions are a significant challenge due to their cytotoxicity, even at low concentrations. To address this issue, various chemosensors have been designed for advanced detection systems, offering simplicity and the potential to differentiate signals from different analytes. Nonetheless, these chemosensors often suffer from limited emission response and complex synthesis procedures. As a result, the tracking and quantification of residual palladium in biological systems and environments r...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Charles Immanuel David Jihyun Lee Karthick Ramanagul Velraj Gothandapani Beom Jin Kim Hyung-Il Lee Source Type: research
Nonparametric estimation of measurement uncertainty arising from sampling
Anal Chim Acta. 2024 May 29;1305:342597. doi: 10.1016/j.aca.2024.342597. Epub 2024 Apr 12.ABSTRACTBACKGROUND: Increasingly, measurement uncertainty has been used by pure and applied analytical chemistry to ensure decision-making in commercial transactions and technical-scientific applications. Until recently, it was considered that measurement uncertainty boiled down to analytical uncertainty; however, over the last two decades, uncertainty arising from sampling has also been considered. However, the second version of the EURACHEM guide, published in 2019, assumes that the frequency distribution is approximately normal or ...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Felipe Rebello Louren ço Elcio Cruz de Oliveira Source Type: research
Ultrasensitive NIR fluorometric assay for inorganic pyrophosphatase detection via Cu < sup > 2+ < /sup > -PPi interaction using bimetallic Au-Ag nanoclusters
Anal Chim Acta. 2024 May 29;1305:342584. doi: 10.1016/j.aca.2024.342584. Epub 2024 Apr 10.ABSTRACTBACKGROUND: Inorganic pyrophosphatase (PPase) is key enzyme playing a key role in biochemical transformations such as biosynthesis of DNA and RNA, bone formation, metabolic pathways associated with lipid, carbohydrate and phosphorous. It has been reported that lung adenocarcinomas, colorectal cancer, and hyperthyroidism disorders can result from abnormal level of PPase. Therefore, it is of notable significance to develop simple and effective real time assay for PPase enzyme activity monitoring for screening of many metabolic p...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Deepika Sharma Nishima Wangoo Rohit K Sharma Source Type: research
Chronoamperometric interrogation of an electrochemical aptamer-based sensor with tetrahedral DNA nanostructure pendulums for continuous biomarker measurements
This study shows chronoamperometry-enabled continuous biomarker monitoring on a sub-second timescale with a drift-free baseline, demonstrating a novel approach to accurately detect molecular dynamics in real time.PMID:38677841 | DOI:10.1016/j.aca.2024.342587 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Yizhou Wang Haowei Duan Yaxiaer Yalikun Shaokoon Cheng Ming Li Source Type: research
A highly sensitive ratiometric fluorescent probe for detecting HSO < sub > 3 < /sub > < sup > - < /sup > /SO < sub > 3 < /sub > < sup > 2- < /sup > and viscosity change based on FRET/TICT mechanism
We reported a unique ratiometric probe QQD for detecting HSO3-/SO32- and viscosity based on the quinolinium skeleton. In addition to detecting HSO3-/SO32- and viscosity change in actual water and food samples, QQD could also monitor the variations of HSO3-/SO32- and viscosity in cells, which provided an experimental basis for further exploration of the role of SO2 derivatives and viscosity in biological systems.PMID:38677842 | DOI:10.1016/j.aca.2024.342588 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Feng-Ting Liu Shu-Mei Zhai Dong-Fang Gao Shu-Hao Yang Bao-Xiang Zhao Zhao-Min Lin Source Type: research
Low voltage electric-double-layer transistor nonenzymic erythromycin sensors based on molecularly imprinted polymers
Anal Chim Acta. 2024 May 29;1305:342589. doi: 10.1016/j.aca.2024.342589. Epub 2024 Apr 10.ABSTRACTErythromycin (Ery) is a commonly used antibiotic that can be found ubiquitously in water bodies. The increasing apprehension over the adverse effects of antibiotic remnants in aquatic environments necessitates the prompt advancement of erythromycin detection techniques that are both highly sensitive and compact. Here, we propose a non-enzyme Ery sensor that integrates a mesoporous SiO2-based low-voltage oxide electric-double-layer transistor (EDLT) with a molecular imprinting technique, featuring a molecularly imprinted polyme...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Linzi Liang Zhengdong Jiang Zhiyuan Luo Kekang Liu Ning Liu Qichang Hu Yanghui Liu Source Type: research
Pinecone derived hierarchical carbon nanostructure as a transducer in a solid-state ion-selective electrode for in vivo analysis of calcium ion
This study presents a new approach to develop sustainable and versatile transducers in solid-state ISEs, a crucial way for in vivo sensing.PMID:38677844 | DOI:10.1016/j.aca.2024.342590 (Source: Analytica Chimica Acta)
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Tongjun Shen Ximin Wang Jiping Ni Ling Ma Lifu Zhang Chunxia Wang Guoyong Huang Source Type: research
Smartphone-based device for rapid and single-step detection of piRNA-651 using anti-DNA:RNA hybrid antibody and enzymatic signal amplification
Anal Chim Acta. 2024 May 29;1305:342583. doi: 10.1016/j.aca.2024.342583. Epub 2024 Apr 11.ABSTRACTP-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs/piRs) are a class of small noncoding RNAs that play a crucial role in regulating various biological processes, including carcinogenesis. One specific piRNA, piR-651, has been reported to be overexpressed in both human blood serum and solid cancer tissues, that can be used a viable biomarker in cancer diagnosis. Early diagnosis of cancer can help reduce the burden of the disease and improve survival rates. In the present work, we report for the first time a smartpho...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Maliana El Aamri Sabrine Baachaoui Hasna Mohammadi Noureddine Raouafi Aziz Amine Source Type: research
Liquid phase detection in the miniature scale. Microfluidic and capillary scale measurement and separation systems. A tutorial review
Anal Chim Acta. 2024 May 29;1305:342507. doi: 10.1016/j.aca.2024.342507. Epub 2024 Mar 23.ABSTRACTMicrofluidic and capillary devices are increasingly being used in analytical applications while their overall size keeps decreasing. Detection sensitivity for these microdevices gains more importance as device sizes and consequently, sample volumes, decrease. This paper reviews optical, electrochemical, electrical, and mass spectrometric detection methods that are applicable to capillary scale and microfluidic devices, with brief introduction to the principles in each case. Much of this is considered in the context of separati...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Cable G Warren Purnendu K Dasgupta Source Type: research
The sphingolipids change in exosomes from cancer patients and association between exosome release and sphingolipids level based on a pseudotargeted lipidomics method
Anal Chim Acta. 2024 May 29;1305:342527. doi: 10.1016/j.aca.2024.342527. Epub 2024 Mar 25.ABSTRACTThe lipid based ESCRT-independent mechanism, which contributes to MVB formation, is one of the crucial procedures in exosome biogenesis. n-SMase is a key lipid metabolism enzyme in this mechanism and can induce the hydrolysis of sphingomyelins (SMs) to ceramides (Cers), thereby promoting the formation of ILVs inside MVBs. Therefore, the regulation of n-SMase can realize the alteration in exosome release. According to the fact that cancer-associated cells have a tendency to release more exosomes than healthy cells, lipid extrac...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Kaige Yang Wenchang Fu Mengjiao Deng Xinyan Li Mingyuan Wu Yan Wang Source Type: research
Discovery of drug targets based on traditional Chinese medicine microspheres (TCM-MPs) fishing strategy combined with bio-layer interferometry (BLI) technology
In this study, a traditional Chinese medicine microspheres (TCM-MPs) target fishing strategy was developed to discover the key drug targets from complex system. The microspheres are composed of Fe3O4 magnetic nanolayer, oleic acid modified layer, the photoaffinity group (4- [3-(Trifluoromethyl)-3H-diazirin-3-yl] benzoic acid, TAD) layer and active small molecule layer from inside to outside. TAD produces highly reactive carbene under ultraviolet light, which can realize the self-assembly and fixation of drug active small molecules with non-selective properties. Here, taking Shenqi Jiangtang Granules (SJG) as an example, th...
Source: Analytica Chimica Acta - April 27, 2024 Category: Chemistry Authors: Hui Zhang Jiangyu Yao Guyu Xiao Jianhui Xie Shuying Mao Chenghong Sun Jingchun Yao Jizhong Yan Pengfei Tu Source Type: research
