Applied Biochemistry and Biotechnology This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Synthesis and Analysis of Euphorbia cognata Boiss-Assisted Organic-Inorganic Complex: Photosynthesis and Stabilization of CoO Nanoparticles
Appl Biochem Biotechnol. 2024 Apr 26. doi: 10.1007/s12010-024-04939-x. Online ahead of print.ABSTRACTSynthesis of metal oxide nanomaterials using phytochemicals has now been regarded as mutually exclusive to chemical synthesis techniques. Here, we have extracted, isolated, and characterized the phytochemicals of Euphorbia cognata Boiss leaf hydro-organic extract and utilized them as biofuel in the preparation of metal oxide nanoparticles (CoO NPs). To evaluate the chemical composition of bio templates, chromatographic techniques like high-performance liquid chromatography (HPLC) and gas chromatography-mass spectroscopy (GC...
Source: Applied Biochemistry and Biotechnology - April 26, 2024 Category: Biochemistry Authors: Sadia Iram Khuram Shahzad Ahmad Irum Shaheen Ahmed M Aljuwayid Ghulam Abbas Ashraf Source Type: research

Ameliorative Effect of Natural Sesquiterpene Alcohol Cedrol Against Cerebral Ischemia Infarction-In Vitro and In Vivo Studies
In this study, we elucidated the neuroprotective properties of cedrol against ischemic infarction in animal and in vitro studies. A cerebral ischemic/reperfusion model was induced in adult Wistar rats, and oxygen-glucose deprivation/reperfusion was induced in SH-SY5Y neuronal cells and treated with different concentrations of cedrol. The percentage of water content, cerebral infarct, and neurological deficit score was assessed in experimental rats. The acetylcholinesterase activity and inflammatory cytokines were quantified to analyze the anti-inflammatory potency of cedrol. Oxidative stress marker malondialdehyde and anti...
Source: Applied Biochemistry and Biotechnology - April 26, 2024 Category: Biochemistry Authors: Xiaohong Hu Mei Han Jing Liu Feng Li Yanchao Cui Source Type: research

Tuning Structural Characteristics of Corn Stover Through Ammonium and Sodium Sulfite (ASS) Pretreatment for Enhanced Enzymatic Hydrolysis
In this study, we have updated the AFEX pretreatment method; ammonia and sodium sulfite (ASS) can be used to produce a more digestible substrate. The results demonstrate that ASS-pretreated corn stover (CS) yields a higher fermentable sugar yield compared with AFEX pretreatment, even at lower enzyme loadings. Specifically, at an enzyme loading of 12 mg protein/g glucan, ASS-CS achieved 88.8% glucose and 80.6% xylose yield. Characterization analysis reveals that lignin underwent sulfonation during ASS pretreatment. This modification results in a more negative zeta potential for ASS-CS, indicating a reduction in nonproductiv...
Source: Applied Biochemistry and Biotechnology - April 26, 2024 Category: Biochemistry Authors: Xiangxue Chen Shizhong Yang Shuiping Ouyang Xinchuan Yuan Junlin Song Shuai Ding Yuanyuan Sha Rui Zhai Source Type: research

Pharmacological Mechanism of Mume Fructus in the Treatment of Triple-Negative Breast Cancer Based on Network Pharmacology
Appl Biochem Biotechnol. 2024 Apr 26. doi: 10.1007/s12010-024-04948-w. Online ahead of print.ABSTRACTOur study aims to find the relevant mechanism of Mume Fructus in the treatment of triple-negative breast cancer (TNBC) by network pharmacology analysis and experimental validation. The effective compounds of Mume Fructus and TNBC-related target genes were imported into Cytoscape to construct a Mume Fructus-effective compounds-disease target network. The common targets of Mume Fructus and TNBC were determined by drawing Venn diagrams. Then, the intersection targets were transferred to the STRING database to construct a prote...
Source: Applied Biochemistry and Biotechnology - April 26, 2024 Category: Biochemistry Authors: Lei Yin Yan Qi Yuting Jiang Source Type: research

Isolation, Identification, and Carbonate Mineralization Characteristics of a Newly Carbonic Anhydrase-Producing Strain
Appl Biochem Biotechnol. 2024 Apr 26. doi: 10.1007/s12010-024-04947-x. Online ahead of print.ABSTRACTCarbonic anhydrase-producing microorganisms can rely on their metabolism for carbon sequestration and carbonate precipitation, which is a relatively effective mode among the known microbially induced carbonate precipitation (MICP) methods. A newly carbonic anhydrase-producing strain was isolated from soil samples. 16S rDNA gene sequencing showed this strain had 99.18% sequence identity to Chryseobacterium gambrini. Various culture parameters (temperature, pH, rotational speed, inoculum size, and metal ions) were optimized f...
Source: Applied Biochemistry and Biotechnology - April 26, 2024 Category: Biochemistry Authors: Jiahua Zhu Jing Sun Caiyan Pang Qian Li Zhihui Yang Guangyue Li Source Type: research

Human Umbilical Cord-Derived Mesenchymal Stem Cells-Exosomes-Delivered miR-375 Targets HDAC4 to Promote Autophagy and Suppress T Cell Apoptosis in Sepsis-Associated Acute Kidney Injury
This study sought to elucidate the mechanism of human umbilical cord-derived mesenchymal stem cells (HUCMSCs)-exosomes (Exos) in sepsis-associated acute kidney injury (SAKI). Exos were isolated from HUCMSCs and co-cultured with CD4+ T cells exposed to lipopolysaccharide to detect the effects of HUCMSCs-Exos on CD4+ T cell apoptosis and autophagy. miR-375 expression in CD4+ T cells and HUCMSCs-Exos was examined. The relationship between miR-375 and HDAC4 was analyzed. A mouse model of SAKI was established and injected with HUCMSCs-Exos to verify the function of HUCMSCs-Exos in vivo. HUCMSCs-Exos inhibited lipopolysaccharide...
Source: Applied Biochemistry and Biotechnology - April 26, 2024 Category: Biochemistry Authors: Min Liu Xiyun Chen Source Type: research

Sustainable Approaches in Green Synthesis of Silica Nanoparticles Using Extracts of Chlorella and Its Application
This study revealed that the nanoparticles can be synthesized using green synthesis methods with low cost, less toxic chemicals, eco-friendly, and have antimicrobial activity against E. coli.PMID:38662321 | DOI:10.1007/s12010-024-04949-9 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - April 25, 2024 Category: Biochemistry Authors: Ragul Manikandan Jibu Thomas Source Type: research

Angiopoietin 1 Relieves Osteolysis by Promoting Macrophage Mitophagy Through the TBK1-SQSTM1 Pathway to Inhibit AIM2 Inflammasome-Mediated Pyroptosis
This study aimed to explore the molecular mechanism by which Angiopoietin (Ang-1) inhibits osteoclast activation to alleviate osteolysis. RAW264.7 mouse macrophages were stimulated with LPS or RANKL to induce osteoclast formation. Additionally, titanium (Ti) particles (50 mg) were subperiosteally implanted around the cranial suture of mice to establish a calvarial osteolysis model. Ang-1, a member of the pro-angiogenic factor protein family and an important inflammatory regulator molecule, was utilized in this model. TRAP staining was utilized to detect osteoclast activation, while a western blot was conducted to identify ...
Source: Applied Biochemistry and Biotechnology - April 25, 2024 Category: Biochemistry Authors: Jian Yin Peng Lai Libo Zhu Jinzhong Ma Source Type: research

CRISPR-Cas13a-Triggered DNAzyme Signal Amplification-Based Colorimetric miRNA Detection Method and Its Application in Evaluating the Anxiety
Appl Biochem Biotechnol. 2024 Apr 23. doi: 10.1007/s12010-024-04951-1. Online ahead of print.ABSTRACTThe development of a bio-sensing strategy based on CRISPR/Cas that is exceptionally sensitive is crucial for the identification of trace molecules. Colorimetric miRNA detection utilizing CRISPR/Cas13a-triggered DNAzyme signal amplification was described in this article. The developed strategy was implemented for miRNA-21 detection as a proof of concept. The cleavage activity of Cas13a was triggered when the target molecule bonded to the Cas13a-crRNA complex and cleaved uracil ribonucleotides (rU) in the substrate probe. As ...
Source: Applied Biochemistry and Biotechnology - April 23, 2024 Category: Biochemistry Authors: Na Yan Zhiwei Hu Lingling Zhang Source Type: research

Synthesis of New Multifunctional Linolenic Acid Vanillyl Ester and Investigation of Antioxidant and Antibacterial Activities
Appl Biochem Biotechnol. 2024 Apr 22. doi: 10.1007/s12010-024-04945-z. Online ahead of print.ABSTRACTVanillyl alcohol (VA) possesses potent antioxidant activity, yet its applicability is hindered by its limited solubility in emulsions or non-polar organic solvents. Conversely, long-chain polyunsaturated fatty acids exhibit antibacterial properties. The combination of these compounds offers the prospect of developing novel phenolic lipid compounds with dual antioxidant and antibacterial activities, alongside enhanced solubility capabilities. In this investigation, linolenic acid vanillyl ester (LAVE) was synthesized from VA...
Source: Applied Biochemistry and Biotechnology - April 22, 2024 Category: Biochemistry Authors: Jennifer Indra Marvella Hyung Kwoun Kim Source Type: research

Mild γ-Butyrolactone/Water Pretreatment for Highly Efficient Sugar Production from Corn Stover
In this study, γ-butyrolactone/water (GBL/H2O) was explored as a mild, efficient, and cost-effective binary solvent pretreatment to enhance hydrolyzability of corn stover (CS). Key pretreatment parameters-reaction time, temperature, and H2SO4 concentration-were systematically investigated for their effects on the physicochemical properties of CS. Specifically, increased temperature and acid concentration significantly decreased cellulose crystallinity (from 1.39 for untreated CS to 1.04 for CS pretreated by GBL/H2O with 100 mM H2SO4 at 120 °C for 1 h) and promoted lignin removal (47.3% for CS pretreated by GBL/H2O with 1...
Source: Applied Biochemistry and Biotechnology - April 8, 2024 Category: Biochemistry Authors: Yu Yang Xueliang Ma Manzhu Wang Xinyi Ji Long Li Ziyu Liu Jiangyao Wang Yujin Ren Lili Jia Source Type: research