Disease-induced changes in bacterial and fungal communities from plant below- and aboveground compartments
In this study, we investigated the bacterial and fungal communities in bulk soil, rhizosphere soil, root, stem, and leaf of both healthy and infected (Potato virus Y disease, PVY) plants. The results indicated that bacterial and fungal communities showed different recruitment strategies in plant organs. The number and abundance of shared bacterial ASVs between bulk and rhizosphere soils decreased with ascending migration from below- to aboveground compartments, while the number and abundance of fungal ASVs showed no obvious changes. Field type, plant compartments, and PVY infection all affected the diversity and structures...
Source: Applied Microbiology and Biotechnology - April 30, 2024 Category: Microbiology Authors: Mingfeng Cao Songqing Huang Jingjing Li Xiaoming Zhang Yi Zhu Jingzhao Sun Li Zhu Yong Deng Jianqiang Xu Zhihua Zhang Qiang Li Jixiang Ai Tian Xie Hengli Li Huaqun Yin Wuyuan Kong Yabing Gu Source Type: research

Disease-induced changes in bacterial and fungal communities from plant below- and aboveground compartments
In this study, we investigated the bacterial and fungal communities in bulk soil, rhizosphere soil, root, stem, and leaf of both healthy and infected (Potato virus Y disease, PVY) plants. The results indicated that bacterial and fungal communities showed different recruitment strategies in plant organs. The number and abundance of shared bacterial ASVs between bulk and rhizosphere soils decreased with ascending migration from below- to aboveground compartments, while the number and abundance of fungal ASVs showed no obvious changes. Field type, plant compartments, and PVY infection all affected the diversity and structures...
Source: Applied Microbiology and Biotechnology - April 30, 2024 Category: Microbiology Authors: Mingfeng Cao Songqing Huang Jingjing Li Xiaoming Zhang Yi Zhu Jingzhao Sun Li Zhu Yong Deng Jianqiang Xu Zhihua Zhang Qiang Li Jixiang Ai Tian Xie Hengli Li Huaqun Yin Wuyuan Kong Yabing Gu Source Type: research

Characterization of a novel GH30 non-specific endoxylanase AcXyn30B from Acetivibrio clariflavus
Appl Microbiol Biotechnol. 2024 Apr 29;108(1):312. doi: 10.1007/s00253-024-13155-w.ABSTRACTThe xylanolytic enzymes Clocl_1795 and Clocl_2746 from glycoside hydrolase (GH) family 30 are highly abundant in the hemicellulolytic system of Acetivibrio clariflavus (Hungateiclostridium, Clostridium clariflavum). Clocl_1795 has been shown to be a xylobiohydrolase AcXbh30A releasing xylobiose from the non-reducing end of xylan and xylooligosaccharides. In this work, biochemical characterization of Clocl_2746 is presented. The protein, designated AcXyn30B, shows low sequence similarity to other GH30 members and phylogenetic analysis...
Source: Applied Microbiology and Biotechnology - April 29, 2024 Category: Microbiology Authors: Katar ína Šuchová Walid Fathallah Vladim ír Puchart Source Type: research

Using multi-omics to explore the effect of Bacillus velezensis SAAS-63 on resisting nutrient stress in lettuce
This study explored the role of the plant growth-promoting rhizosphere bacteria (PGPR) strain Bacillus velezensis SAAS-63 in improving nutrient stress in lettuce. Compared with no inoculation, B. velezensis SAAS-63 inoculants exhibited significantly increased fresh weight, root length, and shoot height under nutrient deficiency, as well as improved antioxidant activities and proline contents. The exogenous addition of B. velezensis SAAS-63 also significantly increased the accumulation of macroelements and micronutrients in lettuce. To elucidate the resistance mechanisms induced by B. velezensis SAAS-63 under nutrient stres...
Source: Applied Microbiology and Biotechnology - April 29, 2024 Category: Microbiology Authors: Yinshuang Bai Ke Song Mengxiang Gao Juan Ma Yifan Zhou Hua Liu Haijuan Zeng Jinbin Wang Xianqing Zheng Source Type: research

In vitro and in vivo fermentation models to study the function of dietary fiber in pig nutrition
Appl Microbiol Biotechnol. 2024 Apr 29;108(1):314. doi: 10.1007/s00253-024-13148-9.ABSTRACTThe importance of dietary fiber (DF) in animal diets is increasing with the advancement of nutritional research. DF is fermented by gut microbiota to produce metabolites, which are important in improving intestinal health. This review is a systematic review of DF in pig nutrition using in vitro and in vivo models. The fermentation characteristics of DF and the metabolic mechanisms of its metabolites were summarized in an in vitro model, and it was pointed out that SCFAs and gases are the important metabolites connecting DF, gut micro...
Source: Applied Microbiology and Biotechnology - April 29, 2024 Category: Microbiology Authors: Weikang Huangfu Shixi Cao Shouren Li Shuhang Zhang Mengqi Liu Boshuai Liu Xiaoyan Zhu Yalei Cui Zhichang Wang Jiangchao Zhao Yinghua Shi Source Type: research

Characterization of a novel GH30 non-specific endoxylanase AcXyn30B from Acetivibrio clariflavus
Appl Microbiol Biotechnol. 2024 Apr 29;108(1):312. doi: 10.1007/s00253-024-13155-w.ABSTRACTThe xylanolytic enzymes Clocl_1795 and Clocl_2746 from glycoside hydrolase (GH) family 30 are highly abundant in the hemicellulolytic system of Acetivibrio clariflavus (Hungateiclostridium, Clostridium clariflavum). Clocl_1795 has been shown to be a xylobiohydrolase AcXbh30A releasing xylobiose from the non-reducing end of xylan and xylooligosaccharides. In this work, biochemical characterization of Clocl_2746 is presented. The protein, designated AcXyn30B, shows low sequence similarity to other GH30 members and phylogenetic analysis...
Source: Applied Microbiology and Biotechnology - April 29, 2024 Category: Microbiology Authors: Katar ína Šuchová Walid Fathallah Vladim ír Puchart Source Type: research

Using multi-omics to explore the effect of Bacillus velezensis SAAS-63 on resisting nutrient stress in lettuce
This study explored the role of the plant growth-promoting rhizosphere bacteria (PGPR) strain Bacillus velezensis SAAS-63 in improving nutrient stress in lettuce. Compared with no inoculation, B. velezensis SAAS-63 inoculants exhibited significantly increased fresh weight, root length, and shoot height under nutrient deficiency, as well as improved antioxidant activities and proline contents. The exogenous addition of B. velezensis SAAS-63 also significantly increased the accumulation of macroelements and micronutrients in lettuce. To elucidate the resistance mechanisms induced by B. velezensis SAAS-63 under nutrient stres...
Source: Applied Microbiology and Biotechnology - April 29, 2024 Category: Microbiology Authors: Yinshuang Bai Ke Song Mengxiang Gao Juan Ma Yifan Zhou Hua Liu Haijuan Zeng Jinbin Wang Xianqing Zheng Source Type: research

In vitro and in vivo fermentation models to study the function of dietary fiber in pig nutrition
Appl Microbiol Biotechnol. 2024 Apr 29;108(1):314. doi: 10.1007/s00253-024-13148-9.ABSTRACTThe importance of dietary fiber (DF) in animal diets is increasing with the advancement of nutritional research. DF is fermented by gut microbiota to produce metabolites, which are important in improving intestinal health. This review is a systematic review of DF in pig nutrition using in vitro and in vivo models. The fermentation characteristics of DF and the metabolic mechanisms of its metabolites were summarized in an in vitro model, and it was pointed out that SCFAs and gases are the important metabolites connecting DF, gut micro...
Source: Applied Microbiology and Biotechnology - April 29, 2024 Category: Microbiology Authors: Weikang Huangfu Shixi Cao Shouren Li Shuhang Zhang Mengqi Liu Boshuai Liu Xiaoyan Zhu Yalei Cui Zhichang Wang Jiangchao Zhao Yinghua Shi Source Type: research

Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A
This study laid a foundation for industrial production and application development of iturin A, and provided the guidance of metabolic engineering breeding for efficient production of other metabolites synthesized by non-ribosomal peptide synthetase. KEY POINTS: • Optimizing 5'-UTR is an effective tactics to regulate synthetase cluster expression. • Blocking 1-DNJ synthesis benefited corn starch utilization and iturin A production. • The iturin A yield attained in this work was the highest yield reported so far.PMID:38676716 | PMC:PMC11055739 | DOI:10.1007/s00253-024-13083-9 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 27, 2024 Category: Microbiology Authors: Menglin She Huijuan Zhou Wanrong Dong Yuxiang Xu Lin Gao Jiaming Gao Yong Yang Zhifan Yang Dongbo Cai Shouwen Chen Source Type: research

Modular metabolic engineering of Bacillus amyloliquefaciens for high-level production of green biosurfactant iturin A
This study laid a foundation for industrial production and application development of iturin A, and provided the guidance of metabolic engineering breeding for efficient production of other metabolites synthesized by non-ribosomal peptide synthetase. KEY POINTS: • Optimizing 5'-UTR is an effective tactics to regulate synthetase cluster expression. • Blocking 1-DNJ synthesis benefited corn starch utilization and iturin A production. • The iturin A yield attained in this work was the highest yield reported so far.PMID:38676716 | DOI:10.1007/s00253-024-13083-9 (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 27, 2024 Category: Microbiology Authors: Menglin She Huijuan Zhou Wanrong Dong Yuxiang Xu Lin Gao Jiaming Gao Yong Yang Zhifan Yang Dongbo Cai Shouwen Chen Source Type: research

Sphingomonas lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic cyanobacterium
This study provided new ideas to understand the interactions between Microcystis and its attached bacteria. KEY POINTS: • A novel bacterium (JXJCY 53 T) was isolated from the cyanosphere of Microcystis sp. FACHB-905 (MF-905) • JXJCY 53 T modulated the growth and microcystin production of MF-905 • MF-905 could control the attached bacteria by specific chemicals other than microcystins (MCs).PMID:38661971 | PMC:PMC11045571 | DOI:10.1007/s00253-024-13081-x (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 25, 2024 Category: Microbiology Authors: Xin Wang Yao Xiao Yang Deng Xue Sang Qing-Lin Deng Le Wang Yi-Wen Yang Bing-Huo Zhang Yu-Qin Zhang Source Type: research

Metabolic modeling of Halomonas campaniensis improves polyhydroxybutyrate production under nitrogen limitation
Appl Microbiol Biotechnol. 2024 Apr 25;108(1):310. doi: 10.1007/s00253-024-13111-8.ABSTRACTPoly-hydroxybutyrate (PHB) is an environmentally friendly alternative for conventional fossil fuel-based plastics that is produced by various microorganisms. Large-scale PHB production is challenging due to the comparatively higher biomanufacturing costs. A PHB overproducer is the haloalkaliphilic bacterium Halomonas campaniensis, which has low nutritional requirements and can grow in cultures with high salt concentrations, rendering it resistant to contamination. Despite its virtues, the metabolic capabilities of H. campaniensis as ...
Source: Applied Microbiology and Biotechnology - April 25, 2024 Category: Microbiology Authors: Carolina Deantas-Jahn Sebasti án N Mendoza Cuauhtemoc Licona-Cassani Camila Orellana Pedro A Saa Source Type: research

Sphingomonas lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic cyanobacterium
This study provided new ideas to understand the interactions between Microcystis and its attached bacteria. KEY POINTS: • A novel bacterium (JXJCY 53 T) was isolated from the cyanosphere of Microcystis sp. FACHB-905 (MF-905) • JXJCY 53 T modulated the growth and microcystin production of MF-905 • MF-905 could control the attached bacteria by specific chemicals other than microcystins (MCs).PMID:38661971 | PMC:PMC11045571 | DOI:10.1007/s00253-024-13081-x (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 25, 2024 Category: Microbiology Authors: Xin Wang Yao Xiao Yang Deng Xue Sang Qing-Lin Deng Le Wang Yi-Wen Yang Bing-Huo Zhang Yu-Qin Zhang Source Type: research

Metabolic modeling of Halomonas campaniensis improves polyhydroxybutyrate production under nitrogen limitation
Appl Microbiol Biotechnol. 2024 Apr 25;108(1):310. doi: 10.1007/s00253-024-13111-8.ABSTRACTPoly-hydroxybutyrate (PHB) is an environmentally friendly alternative for conventional fossil fuel-based plastics that is produced by various microorganisms. Large-scale PHB production is challenging due to the comparatively higher biomanufacturing costs. A PHB overproducer is the haloalkaliphilic bacterium Halomonas campaniensis, which has low nutritional requirements and can grow in cultures with high salt concentrations, rendering it resistant to contamination. Despite its virtues, the metabolic capabilities of H. campaniensis as ...
Source: Applied Microbiology and Biotechnology - April 25, 2024 Category: Microbiology Authors: Carolina Deantas-Jahn Sebasti án N Mendoza Cuauhtemoc Licona-Cassani Camila Orellana Pedro A Saa Source Type: research

Sphingomonas lacusdianchii sp. nov., an attached bacterium inhibited by metabolites from its symbiotic cyanobacterium
This study provided new ideas to understand the interactions between Microcystis and its attached bacteria. KEY POINTS: • A novel bacterium (JXJCY 53 T) was isolated from the cyanosphere of Microcystis sp. FACHB-905 (MF-905) • JXJCY 53 T modulated the growth and microcystin production of MF-905 • MF-905 could control the attached bacteria by specific chemicals other than microcystins (MCs).PMID:38661971 | DOI:10.1007/s00253-024-13081-x (Source: Applied Microbiology and Biotechnology)
Source: Applied Microbiology and Biotechnology - April 25, 2024 Category: Microbiology Authors: Xin Wang Yao Xiao Yang Deng Xue Sang Qing-Lin Deng Le Wang Yi-Wen Yang Bing-Huo Zhang Yu-Qin Zhang Source Type: research