A forced aeration system for microbial culture of multiple shaken vessels suppresses volatilization
Arch Microbiol. 2024 May 5;206(6):246. doi: 10.1007/s00203-024-03960-2.ABSTRACTShake-flask culture, an aerobic submerged culture, has been used in various applications involving cell cultivation. However, it is not designed for forced aeration. Hence, this study aimed to develop a small-scale submerged shaking culture system enabling forced aeration into the medium. A forced aeration control system for multiple vessels allows shaking, suppresses volatilization, and is attachable externally to existing shaking tables. Using a specially developed plug, medium volatilization was reduced to less than 10%, even after 45 h of co...
Source: Archives of Microbiology - May 5, 2024 Category: Microbiology Authors: Masato Takahashi Yoshisuke Sawada Hideki Aoyagi Source Type: research
Optical tweezers for probing the interactions of ZnO and Ag nanoparticles with E. coli
Arch Microbiol. 2024 May 3;206(6):243. doi: 10.1007/s00203-024-03964-y.ABSTRACTThe antibacterial effect of nanoparticles is mainly studied on the ensembles of the bacteria. In contrast, the optical tweezer technique allows the investigation of similar effects on individual bacterium. E. coli is a self-propelled micro-swimmer and ATP-driven active microorganism. In this work, an optical tweezer is employed to examine the mechanical properties of E. coli incubated with ZnO and Ag nanoparticles (NP) in the growth medium. ZnO and Ag NP with a concentration of 10 µg/ml were dispersed in growth medium during active log-growth p...
Source: Archives of Microbiology - May 3, 2024 Category: Microbiology Authors: Sarika B Hinge Arun G Banpurkar Gauri R Kulkarni Source Type: research
Extracellular vesicles from Aggregatibacter actinomycetemcomitans exhibit potential antitumorigenic effects in oral cancer: a comparative in vitro study
Arch Microbiol. 2024 May 3;206(6):244. doi: 10.1007/s00203-024-03976-8.ABSTRACTAggregatibacter actinomycetemcomitans is an opportunistic Gram-negative periodontopathogen strongly associated with periodontitis and infective endocarditis. Recent evidence suggests that periodontopathogens can influence the initiation and progression of oral squamous cell carcinoma (OSCC). Herein we aimed to investigate the effect of A. actinomycetemcomitans-derived extracellular vesicles (EVs) on OSCC cell behavior compared with EVs from periodontopathogens known to associate with carcinogenesis. EVs were isolated from: A. actinomycetemcomita...
Source: Archives of Microbiology - May 3, 2024 Category: Microbiology Authors: Marjut Mets äniitty Shrabon Hasnat Carina Öhman Tuula Salo Kari K Eklund Jan Oscarsson Abdelhakim Salem Source Type: research
β-Carotene production from sugarcane molasses by a newly isolated Rhodotorula toruloides L/24-26-1
Arch Microbiol. 2024 May 3;206(6):245. doi: 10.1007/s00203-024-03973-x.ABSTRACTProduction of carotenoids by yeast fermentation is an advantaged technology due to its easy scaling and safety. Nevertheless, carotenoid production needs an economic culture medium and other efficient yeast stains. The study aims to isolate and identify a yeast strain capable of producing carotenoids using a cost-effective substrate. A new strain was identified as Rhodotorula toruloides L/24-26-1, which can produce carotenoids at different pretreated and unpretreated sugarcane molasses concentrations (40 and 80 g/L). The highest biomass concentr...
Source: Archives of Microbiology - May 3, 2024 Category: Microbiology Authors: Nayra Ochoa-Vi ñals Dania Alonso-Estrada Evelyn Faife-P érez Zhen Chen Georgina Michelena-Alvarez Jos é Luis Martínez-Hernández Ariel Garc ía-Cruz Anna Ilina Source Type: research
Optical tweezers for probing the interactions of ZnO and Ag nanoparticles with E. coli
Arch Microbiol. 2024 May 3;206(6):243. doi: 10.1007/s00203-024-03964-y.ABSTRACTThe antibacterial effect of nanoparticles is mainly studied on the ensembles of the bacteria. In contrast, the optical tweezer technique allows the investigation of similar effects on individual bacterium. E. coli is a self-propelled micro-swimmer and ATP-driven active microorganism. In this work, an optical tweezer is employed to examine the mechanical properties of E. coli incubated with ZnO and Ag nanoparticles (NP) in the growth medium. ZnO and Ag NP with a concentration of 10 µg/ml were dispersed in growth medium during active log-growth p...
Source: Archives of Microbiology - May 3, 2024 Category: Microbiology Authors: Sarika B Hinge Arun G Banpurkar Gauri R Kulkarni Source Type: research
Extracellular vesicles from Aggregatibacter actinomycetemcomitans exhibit potential antitumorigenic effects in oral cancer: a comparative in vitro study
Arch Microbiol. 2024 May 3;206(6):244. doi: 10.1007/s00203-024-03976-8.ABSTRACTAggregatibacter actinomycetemcomitans is an opportunistic Gram-negative periodontopathogen strongly associated with periodontitis and infective endocarditis. Recent evidence suggests that periodontopathogens can influence the initiation and progression of oral squamous cell carcinoma (OSCC). Herein we aimed to investigate the effect of A. actinomycetemcomitans-derived extracellular vesicles (EVs) on OSCC cell behavior compared with EVs from periodontopathogens known to associate with carcinogenesis. EVs were isolated from: A. actinomycetemcomita...
Source: Archives of Microbiology - May 3, 2024 Category: Microbiology Authors: Marjut Mets äniitty Shrabon Hasnat Carina Öhman Tuula Salo Kari K Eklund Jan Oscarsson Abdelhakim Salem Source Type: research
β-Carotene production from sugarcane molasses by a newly isolated Rhodotorula toruloides L/24-26-1
Arch Microbiol. 2024 May 3;206(6):245. doi: 10.1007/s00203-024-03973-x.ABSTRACTProduction of carotenoids by yeast fermentation is an advantaged technology due to its easy scaling and safety. Nevertheless, carotenoid production needs an economic culture medium and other efficient yeast stains. The study aims to isolate and identify a yeast strain capable of producing carotenoids using a cost-effective substrate. A new strain was identified as Rhodotorula toruloides L/24-26-1, which can produce carotenoids at different pretreated and unpretreated sugarcane molasses concentrations (40 and 80 g/L). The highest biomass concentr...
Source: Archives of Microbiology - May 3, 2024 Category: Microbiology Authors: Nayra Ochoa-Vi ñals Dania Alonso-Estrada Evelyn Faife-P érez Zhen Chen Georgina Michelena-Alvarez Jos é Luis Martínez-Hernández Ariel Garc ía-Cruz Anna Ilina Source Type: research
Molecular phylogeny and secondary structure analysis of hop stunt viroid (HSVd) associated with Mulberry (Morus alba) in India
Arch Microbiol. 2024 May 2;206(5):240. doi: 10.1007/s00203-024-03966-w.ABSTRACTHop stunt viroid (HSVd), a small, single stranded, circular, non-coding infectious RNA known to cause infection in various economically important crop plants. In the present investigation, a study was conducted in the southern part of Karnataka districts of India to detect the possible association of HSVd infection in mulberry plants. A total of 41 mulberry plants showing typical viroid-like symptoms along with asymptomatic samples were collected and screened using conventional Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) using a spe...
Source: Archives of Microbiology - May 2, 2024 Category: Microbiology Authors: Shilpa N Teruo Sano Takashi Naoi Janardhana G R Source Type: research
Ureibacillus aquaedulcis sp. nov., isolated from freshwater well and reclassification of Lysinibacillus yapensis and Lysinibacillus antri as Ureibacillus yapensis comb. nov. and Ureibacillus antri comb. Nov
Arch Microbiol. 2024 May 2;206(5):242. doi: 10.1007/s00203-024-03970-0.ABSTRACTA Gram-stain-positive aerobic, rod-shaped, spore-producing bacterium forming colonies with convex elevation and a smooth, intact margin was isolated from a freshwater sample collected from a well situated in an agricultural field. The 16S rRNA gene sequence of the isolated strain BA0131T showed the highest sequence similarity to Lysinibacillus yapensis ylb-03T (99.25%) followed by Ureibacillus chungkukjangi 2RL3-2T (98.91%) and U. sinduriensis BLB-1T (98.65%). The strain BA0131T was oxidase and catalase positive and urease negative. It also test...
Source: Archives of Microbiology - May 2, 2024 Category: Microbiology Authors: Amit Yadav Ruchi Teware Agrima Bhatt Yash Bhavsar Akanksha Maurya Vipool Thorat Venkata Ramana Vemuluri Kiran Kirdat Source Type: research
Co-expression network analysis and identification of core genes in the interaction between wheat and Puccinia striiformis f. sp. tritici
This study can assist scholars in conducting studies on the pathogenesis and help to advance the investigation of wheat-Pst interaction patterns.PMID:38698267 | DOI:10.1007/s00203-024-03925-5 (Source: Archives of Microbiology)
Source: Archives of Microbiology - May 2, 2024 Category: Microbiology Authors: Yibo Wang Ke Zhang Dan Chen Kai Liu Wei Chen Fei He Zhijun Tong Qiaoling Luo Source Type: research
Molecular phylogeny and secondary structure analysis of hop stunt viroid (HSVd) associated with Mulberry (Morus alba) in India
Arch Microbiol. 2024 May 2;206(5):240. doi: 10.1007/s00203-024-03966-w.ABSTRACTHop stunt viroid (HSVd), a small, single stranded, circular, non-coding infectious RNA known to cause infection in various economically important crop plants. In the present investigation, a study was conducted in the southern part of Karnataka districts of India to detect the possible association of HSVd infection in mulberry plants. A total of 41 mulberry plants showing typical viroid-like symptoms along with asymptomatic samples were collected and screened using conventional Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) using a spe...
Source: Archives of Microbiology - May 2, 2024 Category: Microbiology Authors: Shilpa N Teruo Sano Takashi Naoi Janardhana G R Source Type: research
Ureibacillus aquaedulcis sp. nov., isolated from freshwater well and reclassification of Lysinibacillus yapensis and Lysinibacillus antri as Ureibacillus yapensis comb. nov. and Ureibacillus antri comb. Nov
Arch Microbiol. 2024 May 2;206(5):242. doi: 10.1007/s00203-024-03970-0.ABSTRACTA Gram-stain-positive aerobic, rod-shaped, spore-producing bacterium forming colonies with convex elevation and a smooth, intact margin was isolated from a freshwater sample collected from a well situated in an agricultural field. The 16S rRNA gene sequence of the isolated strain BA0131T showed the highest sequence similarity to Lysinibacillus yapensis ylb-03T (99.25%) followed by Ureibacillus chungkukjangi 2RL3-2T (98.91%) and U. sinduriensis BLB-1T (98.65%). The strain BA0131T was oxidase and catalase positive and urease negative. It also test...
Source: Archives of Microbiology - May 2, 2024 Category: Microbiology Authors: Amit Yadav Ruchi Teware Agrima Bhatt Yash Bhavsar Akanksha Maurya Vipool Thorat Venkata Ramana Vemuluri Kiran Kirdat Source Type: research
Co-expression network analysis and identification of core genes in the interaction between wheat and Puccinia striiformis f. sp. tritici
This study can assist scholars in conducting studies on the pathogenesis and help to advance the investigation of wheat-Pst interaction patterns.PMID:38698267 | DOI:10.1007/s00203-024-03925-5 (Source: Archives of Microbiology)
Source: Archives of Microbiology - May 2, 2024 Category: Microbiology Authors: Yibo Wang Ke Zhang Dan Chen Kai Liu Wei Chen Fei He Zhijun Tong Qiaoling Luo Source Type: research
Acidification induce chemical and microbial variation in tea plantation soils and bacterial degradation of the key acidifying phenolic acids
Arch Microbiol. 2024 Apr 30;206(5):239. doi: 10.1007/s00203-024-03858-z.ABSTRACTCamellia sinensis is an important economic plant grown in southern subtropical hilly areas, especially in China, mainly for the production of tea. Soil acidification is a significant cause of the reduction of yield and quality and continuous cropping obstacles in tea plants. Therefore, chemical and microbial properties of tea growing soils were investigated and phenolic acid-degrading bacteria were isolated from a tea plantation. Chemical and ICP-AES investigations showed that the soils tested were acidic, with pH values of 4.05-5.08, and the p...
Source: Archives of Microbiology - April 30, 2024 Category: Microbiology Authors: Yuhan Zhang Binjie Wang Guiwei Wang Zhisheng Zheng Ying Chen Ou Li Yulong Peng Xiufang Hu Source Type: research
Acidification induce chemical and microbial variation in tea plantation soils and bacterial degradation of the key acidifying phenolic acids
Arch Microbiol. 2024 Apr 30;206(5):239. doi: 10.1007/s00203-024-03858-z.ABSTRACTCamellia sinensis is an important economic plant grown in southern subtropical hilly areas, especially in China, mainly for the production of tea. Soil acidification is a significant cause of the reduction of yield and quality and continuous cropping obstacles in tea plants. Therefore, chemical and microbial properties of tea growing soils were investigated and phenolic acid-degrading bacteria were isolated from a tea plantation. Chemical and ICP-AES investigations showed that the soils tested were acidic, with pH values of 4.05-5.08, and the p...
Source: Archives of Microbiology - April 30, 2024 Category: Microbiology Authors: Yuhan Zhang Binjie Wang Guiwei Wang Zhisheng Zheng Ying Chen Ou Li Yulong Peng Xiufang Hu Source Type: research
