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Sequestration of membrane cholesterol by cholesterol-binding proteins inhibits SARS-CoV-2 entry into Vero E6 cells
Biochem Biophys Res Commun. 2024 Apr 16;716:149954. doi: 10.1016/j.bbrc.2024.149954. Online ahead of print.ABSTRACTMembrane lipids and proteins form dynamic domains crucial for physiological and pathophysiological processes, including viral infection. Many plasma membrane proteins, residing within membrane domains enriched with cholesterol (CHOL) and sphingomyelin (SM), serve as receptors for attachment and entry of viruses into the host cell. Among these, human coronaviruses, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), use proteins associated with membrane domains for initial binding and intern...
Source: Biochemical and Biophysical Research communications - May 5, 2024 Category: Biochemistry Authors: Magdalena Kulma Aleksandra Šakanović Apolonija Bedina-Zavec Simon Caserman Ne ža Omersa Ga šper Šolinc Sara Orehek Iva Hafner-Bratkovi č Ur ška Kuhar Brigita Slavec Uro š Krapež Matja ž Ocepek Toshihide Kobayashi Katarzyna Kwiatkowska Roman Jera Source Type: research

Tailored multivalent peptide targeting the B-subunit pentamer of cholera toxin inhibits its intestinal toxicity by inducing aberrant transport of the toxin in cells
Biochem Biophys Res Commun. 2024 Apr 26;716:149991. doi: 10.1016/j.bbrc.2024.149991. Online ahead of print.ABSTRACTCholera toxin (Ctx) is a major virulence factor produced by Vibrio cholerae that can cause gastrointestinal diseases, including severe watery diarrhea and dehydration, in humans. Ctx binds to target cells through multivalent interactions between its B-subunit pentamer and the receptor ganglioside GM1 present on the cell surface. Here, we identified a series of tetravalent peptides that specifically bind to the receptor-binding region of the B-subunit pentamer using affinity-based screening of multivalent rando...
Source: Biochemical and Biophysical Research communications - May 5, 2024 Category: Biochemistry Authors: Miho Watanabe-Takahashi Kahori Kumoi Hiroshi Yamamoto Eiko Shimizu Jun Motoyama Takashi Hamabata Kiyotaka Nishikawa Source Type: research

Structural insights in to the atypical type-I ABC Glucose-6-phosphate importer VCA0625-27 of Vibrio cholerae
Biochem Biophys Res Commun. 2024 May 1;716:150030. doi: 10.1016/j.bbrc.2024.150030. Online ahead of print.ABSTRACTSugar phosphates are potential sources of carbon and phosphate for bacteria. Despite that the process of internalization of Glucose-6-Phosphate (G6P) through plasma membrane remained elusive in several bacteria. VCA0625-27, made of periplasmic ligand binding protein (PLBP) VCA0625, an atypical monomeric permease VCA0626, and a cytosolic ATPase VCA0627, recently emerged as hexose-6-phosphate uptake system of Vibrio cholerae. Here we report high resolution crystal structure of VCA0625 in G6P bound state that larg...
Source: Biochemical and Biophysical Research communications - May 5, 2024 Category: Biochemistry Authors: Indrila Saha Biplab Ghosh Jhimli Dasgupta Source Type: research

The combination of methionine adenosyltransferase 2A inhibitor and methyltransferase like 3 inhibitor promotes apoptosis of non-small cell lung cancer cells and produces synergistic anti-tumor activity
Biochem Biophys Res Commun. 2024 Apr 27;716:150011. doi: 10.1016/j.bbrc.2024.150011. Online ahead of print.ABSTRACTMethionine adenosyltransferase 2 A (MAT2A) mediates the synthesis of methyl donor S-Adenosylmethionine (SAM), providing raw materials for methylation reactions in cells. MAT2A inhibitors are currently used for the treatment of tumors with methylthioadenosine phosphorylase (MTAP) deficiency in clinical research. Methyltransferase like 3 (METTL3) catalyzes N6-methyladenosine (m6A) modification of mRNA in mammalian cells using SAM as the substrate which has been shown to affect the tumorigenesis of non-small cell...
Source: Biochemical and Biophysical Research communications - May 5, 2024 Category: Biochemistry Authors: Yi-Fei Xuan Shan Lu Ying-Jie Ou Xu-Bin Bao Xia-Juan Huan Shan-Shan Song Ze-Hong Miao Ying-Qing Wang Source Type: research

Uric acid-lowering effect of harpagoside and its protective effect against hyperuricemia-induced renal injury in mice
Biochem Biophys Res Commun. 2024 Apr 30;716:150038. doi: 10.1016/j.bbrc.2024.150038. Online ahead of print.ABSTRACTHyperuricemia (HUA) is caused by increased synthesis and/or insufficient excretion of uric acid (UA). Long-lasting HUA may lead to a number of diseases including gout and kidney injury. Harpagoside (Harp) is a bioactive compound with potent anti-inflammatory activity from the roots of Scrophularia ningpoensis. Nevertheless, its potential effect on HUA was not reported. The anti-HUA and nephroprotective effects of Harp on HUA mice were assessed by biochemical and histological analysis. The proteins responsible ...
Source: Biochemical and Biophysical Research communications - May 5, 2024 Category: Biochemistry Authors: Qiong Fu Jin-Juan Zhang Qin-Feng Zhu Ling-Ling Yu Fang Wang Jing Li Xun He Jun-Li Ao Guo-Bo Xu Mao-Chen Wei Xing-Jiang Liao Shang-Gao Liao Source Type: research

Altered mitochondrial Ca 2+ uptake in presynaptic terminals of cultured striatal and cortical neurons from the zQ175 knock-in mouse model of Huntington's disease
Biochem Biophys Res Commun. 2024 Apr 26;716:150010. doi: 10.1016/j.bbrc.2024.150010. Online ahead of print.ABSTRACTCalcium (Ca2+) in mitochondria plays crucial roles in neurons including modulating metabolic processes. Moreover, excessive Ca2+ in mitochondria can lead to cell death. Thus, altered mitochondrial Ca2+ regulation has been implicated in several neurodegenerative diseases including Huntington's disease (HD). HD is a progressive hereditary neurodegenerative disorder that results from abnormally expanded cytosine-adenine-guanine trinucleotide repeats in the huntingtin gene. One neuropathological hallmark of HD is ...
Source: Biochemical and Biophysical Research communications - May 5, 2024 Category: Biochemistry Authors: Hanna Yoo Hyokeun Park Source Type: research

PLD2 deficiency alleviates endothelial glycocalyx degradation in LPS-induced ARDS/ALI
Biochem Biophys Res Commun. 2024 Apr 26;716:150019. doi: 10.1016/j.bbrc.2024.150019. Online ahead of print.ABSTRACT- Acute respiratory distress syndrome (ARDS)/acute lung injury (ALI) is a life-threatening condition marked by severe lung inflammation and increased lung endothelial barrier permeability. Endothelial glycocalyx deterioration is the primary factor of vascular permeability changes in ARDS/ALI. Although previous studies have shown that phospholipase D2 (PLD2) is closely related to the onset and progression of ARDS/ALI, its role and mechanism in the damage of endothelial cell glycocalyx remains unclear. We used L...
Source: Biochemical and Biophysical Research communications - May 4, 2024 Category: Biochemistry Authors: Guiqing Kong Dongxiao Li Xiangyong Liu Jiali Feng Fangyu Ning Xiao Huang Boyang Qi Jianyu Qu Xiaozhi Wang Source Type: research

 1 H β chemical shift-based phase modulated NMR methods for fast identification of amino acid types in proteins
Biochem Biophys Res Commun. 2024 Apr 24;716:150000. doi: 10.1016/j.bbrc.2024.150000. Online ahead of print.ABSTRACTHere we report two phase modulated NMR experiments: PM-2D HN(CACBHB) and PM-2D HN(HB), that use 1Hβ chemical shifts to rapidly identify amino acid type in proteins. The magnetization on the 1Hβ spins during the experiments is allowed to evolve for a fixed evolution period that results in phase modulation (positive or negative) of the cross peaks corresponding to various amino acid residues on their 2D HN projections, resembling a typical 2D [1H-15N]-HSQC spectrum. All amino acids except glycine can be catego...
Source: Biochemical and Biophysical Research communications - May 3, 2024 Category: Biochemistry Authors: Janeka Gartia Ravi Pratap Barnwal Source Type: research

Illuminating intercellular autophagy: A comprehensive review of cell non-autonomous autophagy
Biochem Biophys Res Commun. 2024 Apr 27;716:150024. doi: 10.1016/j.bbrc.2024.150024. Online ahead of print.ABSTRACTMacro-autophagy (autophagy hereafter) is an evolutionarily conserved cellular process that has long been recognized as an intracellular mechanism for maintaining cellular homeostasis. It involves the formation of a membraned structure called the autophagosome, which carries cargo that includes toxic protein aggregates and dysfunctional organelles to the lysosome for degradation and recycling. Autophagy is primarily considered and studied as a cell-autonomous mechanism. However, recent studies have illuminated ...
Source: Biochemical and Biophysical Research communications - May 3, 2024 Category: Biochemistry Authors: Karan Selarka Bhupendra V Shravage Source Type: research

METTL3-mediated m 6 A methylation of SLC38A1 stimulates cervical cancer growth
In conclusion, METTL3-mediated m6A methylation of SLC38A1 stimulates cervical cancer progression. SLC38A1 inhibition is a potential therapeutic strategy for cervical cancer.PMID:38701556 | DOI:10.1016/j.bbrc.2024.150039 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - May 3, 2024 Category: Biochemistry Authors: Hai-Ting Liu Yun Zhao Hong-Cai Wang Qing-Ling Liu Source Type: research

GPR30 selective agonist G-1 induced insulin resistance in ovariectomized mice on high fat diet and its mechanism
CONCLUSION: Despite G-1's the potential efficacy in weight reduction, the concomitant induction of insulin resistance and cardiac impairment in conjunction with an HFD raises significant concerns. Therefore, comprehensive studies of its safety profile and effects under specific conditions are essential prior to clinical use.PMID:38701557 | DOI:10.1016/j.bbrc.2024.150026 (Source: Biochemical and Biophysical Research communications)
Source: Biochemical and Biophysical Research communications - May 3, 2024 Category: Biochemistry Authors: Congcong Lu Da Liu Min Li Xiaocui Shi Jingyue Guan Guoyuan Song Yajuan Yin Mingqi Zheng Fangfang Ma Gang Liu Source Type: research

Subcellular localization of circular RNAs: Where and why
Biochem Biophys Res Commun. 2024 Apr 17;715:149937. doi: 10.1016/j.bbrc.2024.149937. Online ahead of print.ABSTRACTLocalization of RNAs at specific subcellular locations regulating various local cellular events has gained much attention recently. Like most other classes of RNAs, the function of newly discovered circular RNAs (circRNAs) is predominantly determined by their association with different cellular factors in the cell. CircRNAs function as transcriptional and posttranscriptional regulators of gene expression by interacting with transcription factors, splicing regulators, RNA-binding proteins, and microRNAs or by t...
Source: Biochemical and Biophysical Research communications - May 3, 2024 Category: Biochemistry Authors: Susovan Sadhukhan Tanvi Sinha Suchanda Dey Amaresh C Panda Source Type: research