Disposable graphene-oxide screen-printed electrode integrated with portable device for detection of SARS-CoV-2 in clinical samples
Bioelectrochemistry. 2024 Apr 27;158:108722. doi: 10.1016/j.bioelechem.2024.108722. Online ahead of print.ABSTRACTSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnosis is the need of the hour, as cases are persistently increasing, and new variants are constantly emerging. The ever-changing nature of the virus leading to multiple variants, has brought an imminent need for early, accurate and rapid detection methods. Herein, we have reported the design and fabrication of Screen-Printed Electrodes (SPEs) with graphene oxide (GO) as working electrode and modified with specific antibodies for SARS-CoV-2 Recepto...
Source: Bioelectrochemistry - May 2, 2024 Category: Biochemistry Authors: P R Ramya Sayanti Halder K Nagamani Raghuraj Singh Chouhan Sonu Gandhi Source Type: research
Disposable graphene-oxide screen-printed electrode integrated with portable device for detection of SARS-CoV-2 in clinical samples
Bioelectrochemistry. 2024 Apr 27;158:108722. doi: 10.1016/j.bioelechem.2024.108722. Online ahead of print.ABSTRACTSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnosis is the need of the hour, as cases are persistently increasing, and new variants are constantly emerging. The ever-changing nature of the virus leading to multiple variants, has brought an imminent need for early, accurate and rapid detection methods. Herein, we have reported the design and fabrication of Screen-Printed Electrodes (SPEs) with graphene oxide (GO) as working electrode and modified with specific antibodies for SARS-CoV-2 Recepto...
Source: Bioelectrochemistry - May 2, 2024 Category: Biochemistry Authors: P R Ramya Sayanti Halder K Nagamani Raghuraj Singh Chouhan Sonu Gandhi Source Type: research
Biofouling and performance of boron-doped diamond electrodes for detection of dopamine and serotonin in neuron cultivation media
In this study, its performance in electrochemical detection of dopamine and serotonin in neuron cultivation media Neurobasal™ before and after cultivation of rat neurons was investigated. For differential pulse voltammetry the limits of detection in neat Neurobasal™ medium of 2 µM and 0.2 µM for dopamine and serotonin, respectively, were achieved on the polished surface, which is comparable with physiological values. On oxidized surface twofold higher values, but increased repeatabilities of the signals were obtained. However, in Neurobasal™ media with peptides-containing supplements necessary for cell cultivation,...
Source: Bioelectrochemistry - April 30, 2024 Category: Biochemistry Authors: Anton Lytvynenko Simona Baluchov á Ji ří Zima Jan Kr ůšek Karolina Schwarzov á-Pecková Source Type: research
Biofouling and performance of boron-doped diamond electrodes for detection of dopamine and serotonin in neuron cultivation media
In this study, its performance in electrochemical detection of dopamine and serotonin in neuron cultivation media Neurobasal™ before and after cultivation of rat neurons was investigated. For differential pulse voltammetry the limits of detection in neat Neurobasal™ medium of 2 µM and 0.2 µM for dopamine and serotonin, respectively, were achieved on the polished surface, which is comparable with physiological values. On oxidized surface twofold higher values, but increased repeatabilities of the signals were obtained. However, in Neurobasal™ media with peptides-containing supplements necessary for cell cultivation,...
Source: Bioelectrochemistry - April 30, 2024 Category: Biochemistry Authors: Anton Lytvynenko Simona Baluchov á Ji ří Zima Jan Kr ůšek Karolina Schwarzov á-Pecková Source Type: research
Peptide-Based electrochemical potential Scanning: A novel approach for disulfide manipulation in pediatric Respiratory syncytial virus detection
This study introduces an innovative approach using peptides and electrochemical potential scanning for RSV detection. By replacing enzymatic catalysis with electrochemical scanning, the method simplifies the process and reduces costs. Unbound peptides undergo potential-induced disulfide bridge opening, while target-bound peptides remain protected. After removing the target protein, copper ions and a reduced short peptide promote disulfide bridge formation, leading to crosslinking and passivation of the electrode surface. The degree of polymerization and passivation correlates with the target protein levels, generating a si...
Source: Bioelectrochemistry - April 26, 2024 Category: Biochemistry Authors: Jin Yu Yan Li Hui Zou Wenmin Hou Xia Li Lei Zhou Source Type: research
An electrochemiluminescence sensor based on Ag NPs amplifying PDDA-modified TbPO < sub > 4 < /sub > :Ce NWs signal for sensitive detection of lincomycin
Bioelectrochemistry. 2024 Apr 2;158:108702. doi: 10.1016/j.bioelechem.2024.108702. Online ahead of print.ABSTRACTThe residue of lincomycin in water will not only aggravate the drug resistance of bacteria but also cause damage to the human body through biological accumulation. In this work, an electrochemiluminescence (ECL) aptasensor for the detection of lincomycin was constructed based on polydimethyldiallylammonium chloride (PDDA) functionalized Ce-doped TbPO4 nanowires (PDDA-TbPO4:Ce NWs) and silver nanoparticles (Ag NPs). TbPO4:Ce NWs were used as the luminophore, and PDDA was used to functionalize the luminophore to m...
Source: Bioelectrochemistry - April 26, 2024 Category: Biochemistry Authors: Jiajia Zhi Wenchang Wang Xue Mei Qingyi Li Ding Jiang Xueling Shan Xiaohui Chen Zhidong Chen Source Type: research
Peptide-Based electrochemical potential Scanning: A novel approach for disulfide manipulation in pediatric Respiratory syncytial virus detection
This study introduces an innovative approach using peptides and electrochemical potential scanning for RSV detection. By replacing enzymatic catalysis with electrochemical scanning, the method simplifies the process and reduces costs. Unbound peptides undergo potential-induced disulfide bridge opening, while target-bound peptides remain protected. After removing the target protein, copper ions and a reduced short peptide promote disulfide bridge formation, leading to crosslinking and passivation of the electrode surface. The degree of polymerization and passivation correlates with the target protein levels, generating a si...
Source: Bioelectrochemistry - April 26, 2024 Category: Biochemistry Authors: Jin Yu Yan Li Hui Zou Wenmin Hou Xia Li Lei Zhou Source Type: research
An electrochemiluminescence sensor based on Ag NPs amplifying PDDA-modified TbPO < sub > 4 < /sub > :Ce NWs signal for sensitive detection of lincomycin
Bioelectrochemistry. 2024 Apr 2;158:108702. doi: 10.1016/j.bioelechem.2024.108702. Online ahead of print.ABSTRACTThe residue of lincomycin in water will not only aggravate the drug resistance of bacteria but also cause damage to the human body through biological accumulation. In this work, an electrochemiluminescence (ECL) aptasensor for the detection of lincomycin was constructed based on polydimethyldiallylammonium chloride (PDDA) functionalized Ce-doped TbPO4 nanowires (PDDA-TbPO4:Ce NWs) and silver nanoparticles (Ag NPs). TbPO4:Ce NWs were used as the luminophore, and PDDA was used to functionalize the luminophore to m...
Source: Bioelectrochemistry - April 26, 2024 Category: Biochemistry Authors: Jiajia Zhi Wenchang Wang Xue Mei Qingyi Li Ding Jiang Xueling Shan Xiaohui Chen Zhidong Chen Source Type: research
Peptide-Based electrochemical potential Scanning: A novel approach for disulfide manipulation in pediatric Respiratory syncytial virus detection
This study introduces an innovative approach using peptides and electrochemical potential scanning for RSV detection. By replacing enzymatic catalysis with electrochemical scanning, the method simplifies the process and reduces costs. Unbound peptides undergo potential-induced disulfide bridge opening, while target-bound peptides remain protected. After removing the target protein, copper ions and a reduced short peptide promote disulfide bridge formation, leading to crosslinking and passivation of the electrode surface. The degree of polymerization and passivation correlates with the target protein levels, generating a si...
Source: Bioelectrochemistry - April 26, 2024 Category: Biochemistry Authors: Jin Yu Yan Li Hui Zou Wenmin Hou Xia Li Lei Zhou Source Type: research
An electrochemiluminescence sensor based on Ag NPs amplifying PDDA-modified TbPO < sub > 4 < /sub > :Ce NWs signal for sensitive detection of lincomycin
Bioelectrochemistry. 2024 Apr 2;158:108702. doi: 10.1016/j.bioelechem.2024.108702. Online ahead of print.ABSTRACTThe residue of lincomycin in water will not only aggravate the drug resistance of bacteria but also cause damage to the human body through biological accumulation. In this work, an electrochemiluminescence (ECL) aptasensor for the detection of lincomycin was constructed based on polydimethyldiallylammonium chloride (PDDA) functionalized Ce-doped TbPO4 nanowires (PDDA-TbPO4:Ce NWs) and silver nanoparticles (Ag NPs). TbPO4:Ce NWs were used as the luminophore, and PDDA was used to functionalize the luminophore to m...
Source: Bioelectrochemistry - April 26, 2024 Category: Biochemistry Authors: Jiajia Zhi Wenchang Wang Xue Mei Qingyi Li Ding Jiang Xueling Shan Xiaohui Chen Zhidong Chen Source Type: research
Peptide-Based electrochemical potential Scanning: A novel approach for disulfide manipulation in pediatric Respiratory syncytial virus detection
This study introduces an innovative approach using peptides and electrochemical potential scanning for RSV detection. By replacing enzymatic catalysis with electrochemical scanning, the method simplifies the process and reduces costs. Unbound peptides undergo potential-induced disulfide bridge opening, while target-bound peptides remain protected. After removing the target protein, copper ions and a reduced short peptide promote disulfide bridge formation, leading to crosslinking and passivation of the electrode surface. The degree of polymerization and passivation correlates with the target protein levels, generating a si...
Source: Bioelectrochemistry - April 26, 2024 Category: Biochemistry Authors: Jin Yu Yan Li Hui Zou Wenmin Hou Xia Li Lei Zhou Source Type: research
An electrochemiluminescence sensor based on Ag NPs amplifying PDDA-modified TbPO < sub > 4 < /sub > :Ce NWs signal for sensitive detection of lincomycin
Bioelectrochemistry. 2024 Apr 2;158:108702. doi: 10.1016/j.bioelechem.2024.108702. Online ahead of print.ABSTRACTThe residue of lincomycin in water will not only aggravate the drug resistance of bacteria but also cause damage to the human body through biological accumulation. In this work, an electrochemiluminescence (ECL) aptasensor for the detection of lincomycin was constructed based on polydimethyldiallylammonium chloride (PDDA) functionalized Ce-doped TbPO4 nanowires (PDDA-TbPO4:Ce NWs) and silver nanoparticles (Ag NPs). TbPO4:Ce NWs were used as the luminophore, and PDDA was used to functionalize the luminophore to m...
Source: Bioelectrochemistry - April 26, 2024 Category: Biochemistry Authors: Jiajia Zhi Wenchang Wang Xue Mei Qingyi Li Ding Jiang Xueling Shan Xiaohui Chen Zhidong Chen Source Type: research
Enhanced recognition of G-quadruplex DNA oxidative damage based on DNA-mediated charge transfer
Bioelectrochemistry. 2024 Apr 21;158:108714. doi: 10.1016/j.bioelechem.2024.108714. Online ahead of print.ABSTRACTG-quadruplex (G4) DNA is present in human telomere oligonucleotide sequences. Oxidative damage to telomeric DNA accelerates telomere shortening, which is strongly associated with aging and cancer. Most of the current analyses on oxidative DNA damage are based on ds-DNA. Here, we developed a electrochemiluminescence (ECL) probe for enhanced recognition of oxidative damage in G4-DNA based on DNA-mediated charge transfer (CT), which could specifically recognize damaged sites depending on the position of 8-oxoguani...
Source: Bioelectrochemistry - April 23, 2024 Category: Biochemistry Authors: Zhangjin He Ziqi Wang Liping Lu Xiayan Wang Guangsheng Guo Source Type: research
Continuous high-purity bioelectrochemical nitrogen recovery from high N-loaded wastewaters
Bioelectrochemistry. 2024 Apr 16;158:108707. doi: 10.1016/j.bioelechem.2024.108707. Online ahead of print.ABSTRACTMicrobial electrolysis cells (MEC) have been identified as an energy efficient system for ammonium recovery from wastewater. However, high ammonium concentrations at the anode can have inhibitory effects. This work aims to determine the effects on current generation performance and active ammonia nitrogen recovery in wastewater containing 0.5 to 2.5 g N-NH4+/L. The study also evaluates the effect of two cathode materials, stainless steel (SS-MEC) and nickel foam (NF-MEC). When the concentration of ammonium in t...
Source: Bioelectrochemistry - April 23, 2024 Category: Biochemistry Authors: Zainab Ul Mira Sulonen Juan Antonio Baeza Albert Guisasola Source Type: research
Enhanced recognition of G-quadruplex DNA oxidative damage based on DNA-mediated charge transfer
Bioelectrochemistry. 2024 Apr 21;158:108714. doi: 10.1016/j.bioelechem.2024.108714. Online ahead of print.ABSTRACTG-quadruplex (G4) DNA is present in human telomere oligonucleotide sequences. Oxidative damage to telomeric DNA accelerates telomere shortening, which is strongly associated with aging and cancer. Most of the current analyses on oxidative DNA damage are based on ds-DNA. Here, we developed a electrochemiluminescence (ECL) probe for enhanced recognition of oxidative damage in G4-DNA based on DNA-mediated charge transfer (CT), which could specifically recognize damaged sites depending on the position of 8-oxoguani...
Source: Bioelectrochemistry - April 23, 2024 Category: Biochemistry Authors: Zhangjin He Ziqi Wang Liping Lu Xiayan Wang Guangsheng Guo Source Type: research
