Contribution of the seminal microbiome to paternal programming
Biol Reprod. 2024 May 2:ioae068. doi: 10.1093/biolre/ioae068. Online ahead of print.ABSTRACTThe field of Developmental Origins of Health and Disease (DOHaD) has primarily focused on maternal programming of offspring health. However, emerging evidence suggests that paternal factors, including the seminal microbiome, could potentially play important roles in shaping the developmental trajectory and long-term offspring health outcomes. Historically, the microbes present in the semen were regarded as inherently pathogenic agents. However, this dogma has recently been challenged by the discovery of a diverse commensal microbial...
Source: Biology of Reproduction - May 2, 2024 Category: Reproduction Medicine Authors: Justine Kilama Carl R Dahlen Lawrence P Reynolds Samat Amat Source Type: research
Machine learning in time-lapse imaging to differentiate embryos from young vs old mice
This study employs time-lapse microscopy and machine learning to elucidate changes in embryonic growth kinetics with maternal aging. We analyzed morphokinetic parameters of embryos from young and aged C57BL6/NJ mice via continuous imaging. Our findings show that aged embryos accelerated through cleavage stages (from 5-cells) to morula compared to younger counterparts, with no significant differences observed in later stages of blastulation. Unsupervised machine learning identified two distinct clusters comprising of embryos from aged or young donors. Moreover, in supervised learning, the XGBoost (extreme gradient boosting)...
Source: Biology of Reproduction - April 30, 2024 Category: Reproduction Medicine Authors: Liubin Yang Carolina Leynes Ashley Pawelka Isabel Lorenzo Andrew Chou Brendan Lee Jason D Heaney Source Type: research
The long non-coding RPPH1 is decreased in leukocytes and increased in plasma from women developing pre-eclampsia
In conclusion, RPPH1 expression in leukocytes was significantly decreased in women with pre-eclampsia, and the expression at 22-24 weeks associated with the subsequent development of pre-eclampsia. RPPH1 in leukocytes may be a useful biomarker for prediction and/or early detection of pre-eclampsia and an unknown regulator of the signaling affecting immune cells.PMID:38685609 | DOI:10.1093/biolre/ioae069 (Source: Biology of Reproduction)
Source: Biology of Reproduction - April 30, 2024 Category: Reproduction Medicine Authors: Dina-Marie Munkelien Myhrer Monica Fr øystad Marie Cecilie Paasche Roland Thor Ueland Tove Lekva Source Type: research
Comparison of assay methods for quantifying sex hormone concentrations across the menstrual cycle in rhesus macaques
Biol Reprod. 2024 Apr 30:ioae063. doi: 10.1093/biolre/ioae063. Online ahead of print.ABSTRACTImmunoassays have been the preferred method for steroid hormone analysis for more than 50 years. Automated immunoassays (AIAs) offer high-throughput, rapid data turnaround, and low cost for measuring steroid hormone concentrations. The application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for steroid quantification provides greater specificity and selectivity for individual steroids, the ability to simultaneously analyze multiple steroids, and high-throughput and automation. We compared AIA and LC-MS/MS for analy...
Source: Biology of Reproduction - April 30, 2024 Category: Reproduction Medicine Authors: Kristopher M Fecteau Andrea J Winchell Steven W Blue Maria Luisa Appleman Henryk F Urbanski David W Erikson Source Type: research
Machine learning in time-lapse imaging to differentiate embryos from young vs old mice
This study employs time-lapse microscopy and machine learning to elucidate changes in embryonic growth kinetics with maternal aging. We analyzed morphokinetic parameters of embryos from young and aged C57BL6/NJ mice via continuous imaging. Our findings show that aged embryos accelerated through cleavage stages (from 5-cells) to morula compared to younger counterparts, with no significant differences observed in later stages of blastulation. Unsupervised machine learning identified two distinct clusters comprising of embryos from aged or young donors. Moreover, in supervised learning, the XGBoost (extreme gradient boosting)...
Source: Biology of Reproduction - April 30, 2024 Category: Reproduction Medicine Authors: Liubin Yang Carolina Leynes Ashley Pawelka Isabel Lorenzo Andrew Chou Brendan Lee Jason D Heaney Source Type: research
The long non-coding RPPH1 is decreased in leukocytes and increased in plasma from women developing pre-eclampsia
In conclusion, RPPH1 expression in leukocytes was significantly decreased in women with pre-eclampsia, and the expression at 22-24 weeks associated with the subsequent development of pre-eclampsia. RPPH1 in leukocytes may be a useful biomarker for prediction and/or early detection of pre-eclampsia and an unknown regulator of the signaling affecting immune cells.PMID:38685609 | DOI:10.1093/biolre/ioae069 (Source: Biology of Reproduction)
Source: Biology of Reproduction - April 30, 2024 Category: Reproduction Medicine Authors: Dina-Marie Munkelien Myhrer Monica Fr øystad Marie Cecilie Paasche Roland Thor Ueland Tove Lekva Source Type: research
Comparison of assay methods for quantifying sex hormone concentrations across the menstrual cycle in rhesus macaques
Biol Reprod. 2024 Apr 30:ioae063. doi: 10.1093/biolre/ioae063. Online ahead of print.ABSTRACTImmunoassays have been the preferred method for steroid hormone analysis for more than 50 years. Automated immunoassays (AIAs) offer high-throughput, rapid data turnaround, and low cost for measuring steroid hormone concentrations. The application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for steroid quantification provides greater specificity and selectivity for individual steroids, the ability to simultaneously analyze multiple steroids, and high-throughput and automation. We compared AIA and LC-MS/MS for analy...
Source: Biology of Reproduction - April 30, 2024 Category: Reproduction Medicine Authors: Kristopher M Fecteau Andrea J Winchell Steven W Blue Maria Luisa Appleman Henryk F Urbanski David W Erikson Source Type: research
The interaction of ER stress and autophagy in trophoblasts: navigating pregnancy outcome
Biol Reprod. 2024 Apr 28:ioae066. doi: 10.1093/biolre/ioae066. Online ahead of print.ABSTRACTThe endoplasmic reticulum (ER) is a complex and dynamic organelle that initiates unfolded protein response (UPR) and endoplasmic reticulum stress (ER Stress) in response to the accumulation of unfolded or misfolded proteins within its lumen. Autophagy is a paramount intracellular degradation system that facilitates the transportation of proteins, cytoplasmic components, and organelles to lysosomes for degradation and recycling. Preeclampsia (PE) and intrauterine growth retardation (IUGR) are two common complications of pregnancy as...
Source: Biology of Reproduction - April 28, 2024 Category: Reproduction Medicine Authors: Yi Zheng Xia Zha Bei Zhang Mabrouk Elsabagh Hongrong Wang Mengzhi Wang Hao Zhang Source Type: research
The interaction of ER stress and autophagy in trophoblasts: navigating pregnancy outcome
Biol Reprod. 2024 Apr 28:ioae066. doi: 10.1093/biolre/ioae066. Online ahead of print.ABSTRACTThe endoplasmic reticulum (ER) is a complex and dynamic organelle that initiates unfolded protein response (UPR) and endoplasmic reticulum stress (ER Stress) in response to the accumulation of unfolded or misfolded proteins within its lumen. Autophagy is a paramount intracellular degradation system that facilitates the transportation of proteins, cytoplasmic components, and organelles to lysosomes for degradation and recycling. Preeclampsia (PE) and intrauterine growth retardation (IUGR) are two common complications of pregnancy as...
Source: Biology of Reproduction - April 28, 2024 Category: Reproduction Medicine Authors: Yi Zheng Xia Zha Bei Zhang Mabrouk Elsabagh Hongrong Wang Mengzhi Wang Hao Zhang Source Type: research
The interaction of ER stress and autophagy in trophoblasts: navigating pregnancy outcome
Biol Reprod. 2024 Apr 28:ioae066. doi: 10.1093/biolre/ioae066. Online ahead of print.ABSTRACTThe endoplasmic reticulum (ER) is a complex and dynamic organelle that initiates unfolded protein response (UPR) and endoplasmic reticulum stress (ER Stress) in response to the accumulation of unfolded or misfolded proteins within its lumen. Autophagy is a paramount intracellular degradation system that facilitates the transportation of proteins, cytoplasmic components, and organelles to lysosomes for degradation and recycling. Preeclampsia (PE) and intrauterine growth retardation (IUGR) are two common complications of pregnancy as...
Source: Biology of Reproduction - April 28, 2024 Category: Reproduction Medicine Authors: Yi Zheng Xia Zha Bei Zhang Mabrouk Elsabagh Hongrong Wang Mengzhi Wang Hao Zhang Source Type: research
Lrp13a and Lrp13b serve as vitellogenin receptors in the ovary of zebrafish †
Biol Reprod. 2024 Apr 24:ioae040. doi: 10.1093/biolre/ioae040. Online ahead of print.ABSTRACTIn oviparous animals, egg yolk is largely derived from vitellogenin, which is taken up from the maternal circulation by the growing oocytes via the vitellogenin receptor. Recently, a novel member of the lipoprotein receptor superfamily termed low-density lipoprotein receptor-related protein 13 was identified and proposed as a candidate of vitellogenin receptor in oviparous animals. However, the roles of low-density lipoprotein receptor-related protein 13 in vitellogenesis are still poorly defined. Here, we investigated the expressi...
Source: Biology of Reproduction - April 25, 2024 Category: Reproduction Medicine Authors: Zhiquan Liu Nan Zhang Chuangxin Wang Lina Shi Yixuan Hu Yamei Wang Jianzhen Li Source Type: research
Factors defining developmental competence of bovine oocytes collected for in vitro embryo production
Biol Reprod. 2024 Apr 25:ioae065. doi: 10.1093/biolre/ioae065. Online ahead of print.ABSTRACTDespite the currently relatively low effectiveness of producing bovine embryos in vitro, there is a growing interest in applying this laboratory method in the field of reproduction. Many aspects of the procedure need to be improved. One of the main problems is the inferior developmental competence of in vitro matured oocytes that are collected using the ovum pick-up (OPU) method. The mechanisms of oocyte capacitation and maturation, as well as the in vivo conditions in which they grow and mature, should be carefully analyzed. A del...
Source: Biology of Reproduction - April 25, 2024 Category: Reproduction Medicine Authors: Aleksandra Teresa Pytel Krystyna Żyżyńska-Galeńska Zdzis ław Gajewski Krzysztof Papis Source Type: research
Lrp13a and Lrp13b serve as vitellogenin receptors in the ovary of zebrafish †
Biol Reprod. 2024 Apr 24:ioae040. doi: 10.1093/biolre/ioae040. Online ahead of print.ABSTRACTIn oviparous animals, egg yolk is largely derived from vitellogenin, which is taken up from the maternal circulation by the growing oocytes via the vitellogenin receptor. Recently, a novel member of the lipoprotein receptor superfamily termed low-density lipoprotein receptor-related protein 13 was identified and proposed as a candidate of vitellogenin receptor in oviparous animals. However, the roles of low-density lipoprotein receptor-related protein 13 in vitellogenesis are still poorly defined. Here, we investigated the expressi...
Source: Biology of Reproduction - April 25, 2024 Category: Reproduction Medicine Authors: Zhiquan Liu Nan Zhang Chuangxin Wang Lina Shi Yixuan Hu Yamei Wang Jianzhen Li Source Type: research
Factors defining developmental competence of bovine oocytes collected for in vitro embryo production
Biol Reprod. 2024 Apr 25:ioae065. doi: 10.1093/biolre/ioae065. Online ahead of print.ABSTRACTDespite the currently relatively low effectiveness of producing bovine embryos in vitro, there is a growing interest in applying this laboratory method in the field of reproduction. Many aspects of the procedure need to be improved. One of the main problems is the inferior developmental competence of in vitro matured oocytes that are collected using the ovum pick-up (OPU) method. The mechanisms of oocyte capacitation and maturation, as well as the in vivo conditions in which they grow and mature, should be carefully analyzed. A del...
Source: Biology of Reproduction - April 25, 2024 Category: Reproduction Medicine Authors: Aleksandra Teresa Pytel Krystyna Żyżyńska-Galeńska Zdzis ław Gajewski Krzysztof Papis Source Type: research
Lrp13a and Lrp13b serve as vitellogenin receptors in the ovary of zebrafish †
Biol Reprod. 2024 Apr 24:ioae040. doi: 10.1093/biolre/ioae040. Online ahead of print.ABSTRACTIn oviparous animals, egg yolk is largely derived from vitellogenin, which is taken up from the maternal circulation by the growing oocytes via the vitellogenin receptor. Recently, a novel member of the lipoprotein receptor superfamily termed low-density lipoprotein receptor-related protein 13 was identified and proposed as a candidate of vitellogenin receptor in oviparous animals. However, the roles of low-density lipoprotein receptor-related protein 13 in vitellogenesis are still poorly defined. Here, we investigated the expressi...
Source: Biology of Reproduction - April 25, 2024 Category: Reproduction Medicine Authors: Zhiquan Liu Nan Zhang Chuangxin Wang Lina Shi Yixuan Hu Yamei Wang Jianzhen Li Source Type: research
