Apoptotic-Related MiRNAs Correlated with Functional and Flow Cytometric Parameters in Asthenozoospermic Holstein Bulls After Freeze-Thaw Process
Biopreserv Biobank. 2024 Apr 30. doi: 10.1089/bio.2023.0135. Online ahead of print.ABSTRACTMany cellular processes in spermatozoa, including apoptosis and motility, are regulated by miRNA. Different miRNAs and molecular pathways are involved in asthenozoospermia (AS) conditions, which are thought to be one of the causes of infertility with reduced sperm motility. Thirty-two semen samples from four Holstein bulls with normozoospermia (NS), total motility ≥ 70%, and progressive motility ≥ 60%, and 32 semen samples from four bulls with AS, total motility ≤ 40%, and progressive motility ≤ 32% were used to investigate t...
Source: Biopreservation and Biobanking - April 30, 2024 Category: Biomedical Science Authors: Morteza Taravat Amirmahdi Roshanzamir Maryam Rahbar Tohid Rezaei Topraggaleh Reza Asadpour Mustafa Numan Bucak Source Type: research
Stability and Qualification of a Legacy Fungal Collection
Conclusion: Our findings demonstrated the potential value of reviving historical microbial collections for biobanking and research activities and reassure us about the collection's future reopening.PMID:38686572 | DOI:10.1089/bio.2023.0154 (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 30, 2024 Category: Biomedical Science Authors: Mennat El Ghalid Adriana Chiarelli Sylvain Brisse Fay Betsou Dea Garcia-Hermoso Source Type: research
A Comparative Study of B Cell Blast Isolation Methods from Bone Marrow Aspirates of Pediatric Leukemia Patients
Biopreserv Biobank. 2024 Apr 30. doi: 10.1089/bio.2023.0133. Online ahead of print.ABSTRACTDensity gradient centrifugation is a conventional technique widely utilized to isolate bone marrow mononuclear cells (BM-MNC) from bone marrow (BM) aspirates obtained from pediatric B-cell acute lymphoblastic leukemia (B-ALL) patients. Nevertheless, this technique achieves incomplete recovery of mononuclear cells and is relatively time-consuming and expensive. Given that B-ALL is the most common childhood malignancy, alternative methods for processing B-ALL samples may be more cost-effective. In this pilot study, we use several reado...
Source: Biopreservation and Biobanking - April 30, 2024 Category: Biomedical Science Authors: Tanmaya Atre Vi Nguyen Veronica Chow Gregor S D Reid Suzanne Vercauteren Source Type: research
Apoptotic-Related MiRNAs Correlated with Functional and Flow Cytometric Parameters in Asthenozoospermic Holstein Bulls After Freeze-Thaw Process
Biopreserv Biobank. 2024 Apr 30. doi: 10.1089/bio.2023.0135. Online ahead of print.ABSTRACTMany cellular processes in spermatozoa, including apoptosis and motility, are regulated by miRNA. Different miRNAs and molecular pathways are involved in asthenozoospermia (AS) conditions, which are thought to be one of the causes of infertility with reduced sperm motility. Thirty-two semen samples from four Holstein bulls with normozoospermia (NS), total motility ≥ 70%, and progressive motility ≥ 60%, and 32 semen samples from four bulls with AS, total motility ≤ 40%, and progressive motility ≤ 32% were used to investigate t...
Source: Biopreservation and Biobanking - April 30, 2024 Category: Biomedical Science Authors: Morteza Taravat Amirmahdi Roshanzamir Maryam Rahbar Tohid Rezaei Topraggaleh Reza Asadpour Mustafa Numan Bucak Source Type: research
Stability and Qualification of a Legacy Fungal Collection
Conclusion: Our findings demonstrated the potential value of reviving historical microbial collections for biobanking and research activities and reassure us about the collection's future reopening.PMID:38686572 | DOI:10.1089/bio.2023.0154 (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 30, 2024 Category: Biomedical Science Authors: Mennat El Ghalid Adriana Chiarelli Sylvain Brisse Fay Betsou Dea Garcia-Hermoso Source Type: research
A Comparative Study of B Cell Blast Isolation Methods from Bone Marrow Aspirates of Pediatric Leukemia Patients
Biopreserv Biobank. 2024 Apr 30. doi: 10.1089/bio.2023.0133. Online ahead of print.ABSTRACTDensity gradient centrifugation is a conventional technique widely utilized to isolate bone marrow mononuclear cells (BM-MNC) from bone marrow (BM) aspirates obtained from pediatric B-cell acute lymphoblastic leukemia (B-ALL) patients. Nevertheless, this technique achieves incomplete recovery of mononuclear cells and is relatively time-consuming and expensive. Given that B-ALL is the most common childhood malignancy, alternative methods for processing B-ALL samples may be more cost-effective. In this pilot study, we use several reado...
Source: Biopreservation and Biobanking - April 30, 2024 Category: Biomedical Science Authors: Tanmaya Atre Vi Nguyen Veronica Chow Gregor S D Reid Suzanne Vercauteren Source Type: research
The Information Technology (IT) Infrastructure of the Multicenter Archipelago of Ovarian Cancer Research Biobank: A Potential Blueprint for Other Biobanks
Conclusion: The IT infrastructure of the AOCR biobank represents a new standard for biobanks, offering flexibility to handle diverse diseases and types of biomaterials. This infrastructure bypasses the need for disease-specific, custom-built software, thereby being cost- and time-effective while ensuring data quality and legislative compliance. The adaptability of this infrastructure highlights its potential to serve as a blueprint for the development of IT infrastructures in both new and existing biobanks.PMID:38682281 | DOI:10.1089/bio.2023.0118 (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 29, 2024 Category: Biomedical Science Authors: Hein S Zelisse Sander de Ridder Mignon D J M van Gent Constantijne H Mom G Bea A Wisman Eva-Maria Roes Anna K L Reyners Jurgen M Piek Gatske M Nieuwenhuyzen-de Boer Christianne A R Lok Cornelis D de Kroon Loes F S Kooreman Marc-Jan Janssen Maurice Phm Jan Source Type: research
An Approach to Evaluate the Costs and Outputs of Academic Biobanks
Biopreserv Biobank. 2024 Apr 26. doi: 10.1089/bio.2023.0112. Online ahead of print.ABSTRACTAcademic biobanks commonly report sustainability challenges, which may be exacerbated by a lack of information on biobank value. To better understand the costs and supported outputs that contribute to biobank value, we developed a systematic, generalizable methodology to determine biobank inputs and publications arising from biobank-supported research. We then tested this in a small cohort (n = 12) of academic cancer biobanks in New South Wales, Australia. A proforma was developed to capture monetary and in-kind biobank costing data ...
Source: Biopreservation and Biobanking - April 26, 2024 Category: Biomedical Science Authors: Amanda Rush NSW Biobank Study Group Daniel R Catchpoole Peter H Watson Jennifer A Byrne Source Type: research
A Biobank Internship: Focusing on Undergraduates' Hands-on Experience in LMICs
Biopreserv Biobank. 2024 Apr 25. doi: 10.1089/bio.2024.0043. Online ahead of print.NO ABSTRACTPMID:38661259 | DOI:10.1089/bio.2024.0043 (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 25, 2024 Category: Biomedical Science Authors: Amr M Abdelhameed Hadeer Ghonim Suha A Farraj Remon S Azer Maryam Awadh Laila Gadelrub Source Type: research
Delta-S-Cys-Albumin as a Marker of Pediatric Biospecimen Integrity
We report application of a previously developed assay in adults-the ΔS-Cys-Albumin assay, which facilitates estimation of plasma and serum exposure to thawed conditions-to a population of pediatric EDTA plasma samples from patients aged 3-18 years to determine the assay's applicability, estimate its reference range for pediatric samples, and assess the impact of pre-centrifugation delay at 0°C. In addition, the effect of plasma thawed-state exposure to a range of times at 23°C, 4°C, and -20°C on ΔS-Cys-Albumin was evaluated. Using 98 precollected and processed pediatric EDTA plasma specimens, no difference was found ...
Source: Biopreservation and Biobanking - April 23, 2024 Category: Biomedical Science Authors: Schuyler Kremer Valentina Shakhnovich Amanda K Riffel Lisa Harvey Chad R Borges Source Type: research
Effect of Elamipretide on the Vitrification of Mouse Ovarian Tissue by Freezing
In conclusion, SS-31 improved the cryopreservation of ovarian tissues, and 100 μmol/L SS-31 was found to be the most effective.PMID:38648553 | DOI:10.1089/bio.2023.0078 (Source: Biopreservation and Biobanking)
Source: Biopreservation and Biobanking - April 22, 2024 Category: Biomedical Science Authors: Xingfeng Yao Qingfang Lu Yuyin Wu Juan Liu Niang Liu Xiling Huang Changlong Xu Source Type: research
