Bioprocess and Biosystems Engineering This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC)
Bioprocess Biosyst Eng. 2024 Apr 25. doi: 10.1007/s00449-024-03015-2. Online ahead of print.ABSTRACTStreptomyces produce a broad spectrum of biologically active molecules such as oxytetracycline and rimocidin, which are widely used in human and animal treatments. microparticle-enhanced cultivation (MPEC) is one of the tools used for Streptomyces bioprocesses intensification by the control of mycelial morphology. In the present work, morphological changes of Streptomyces rimosus caused by the addition of 10 µm talc microparticles in MPEC were correlated with the biosynthetic activity of the microorganism. Comparing the run...
Source: Bioprocess and Biosystems Engineering - April 25, 2024 Category: Biomedical Engineering Authors: Anna Ścigaczewska Tomasz Boruta Marcin Bizukoj ć Source Type: research

Impact of fed-batch process intensification on the productivity and product quality of two CHO cell lines expressing unique novel molecular format proteins
In this study, the impact of a high inoculation density (HID) fed-batch process on the productivity and product quality attributes of two CHO cell lines expressing unique NMFs, a monospecific antibody with an Fc-fusion protein and a bispecific antibody, compared to low inoculation density (LID) platform fed-batch processes was evaluated. It was observed that an intensified platform fed-batch process increased product concentrations by 33 and 109% for the two uniquely structured complex proteins in a shorter culture duration while maintaining similar product quality attributes to traditional fed-batch processes.PMID:3865384...
Source: Bioprocess and Biosystems Engineering - April 23, 2024 Category: Biomedical Engineering Authors: Nicolas Q Wolnick Marissa R Dickson Thaddaeus A Webster Roger P Connolly Nancy Fernandes Vesela Encheva Hunter Crittenden Jessica Hodgkins Brian C Hadley Gabriella Palermo Shannon J Hendrick Roy A Newell Genevieve Gray Christian Siltanen Julia Armstrong B Source Type: research

Ultrasonic synthesis of green lipid nanocarriers loaded with Scutellaria barbata extract: a sustainable approach for enhanced anticancer and antibacterial therapy
This study presents the novel ultrasonic preparation of lipid nanocarriers loaded with Scutellaria barbata extract, repurposed for anticancer and antibacterial use. High-frequency ultrasonic waves enabled the precise self-assembly of DSPE-PEG, Span 40, and cholesterol to form nanocarriers encapsulating the therapeutic extract without the use of toxic solvents, exemplifying green nanotechnology. Leveraging the inherent anticancer and antibacterial properties of Scutellaria barbata, the study demonstrates that lipid encapsulation enhances the bioavailability and controlled release of the extract, which is vital for its thera...
Source: Bioprocess and Biosystems Engineering - April 22, 2024 Category: Biomedical Engineering Authors: Raghu Jetti Maritza Lucia Vaca C árdenas Haider Falih Shamikh Al-Saedi Shaymaa Abed Hussein Hussam Abdali Abdulridui Salah Hassan Zain Al-Abdeen Usama Kadem Radi Adnan Hashim Abdulkadhim Sahar Balkit Hussein Ahmed Alawadi Ali Alsalamy Source Type: research

A catalytic membrane approach as a way to obtain sweet and unsweet lactose-free milk
Bioprocess Biosyst Eng. 2024 Apr 22. doi: 10.1007/s00449-024-03018-z. Online ahead of print.ABSTRACTThe growing need in the current market for innovative solutions to obtain lactose-free (L-F) milk is caused by the annual increase in the prevalence of lactose intolerance inside as well as the newborn, children, and adults. Various configurations of enzymes can yield two distinct L-F products: sweet (β-galactosidase) and unsweet (β-galactosidase and glucose oxidase) L-F milk. In addition, the reduction of sweetness through glucose decomposition should be performed in a one-pot mode with catalase to eliminate product inhib...
Source: Bioprocess and Biosystems Engineering - April 21, 2024 Category: Biomedical Engineering Authors: Katarzyna Czy żewska Anna Trusek Source Type: research

Analysis of the role of perfusion, mechanical, and electrical stimulation in bioreactors for cardiac tissue engineering
Bioprocess Biosyst Eng. 2024 Apr 20. doi: 10.1007/s00449-024-03004-5. Online ahead of print.ABSTRACTSince cardiovascular diseases (CVDs) are globally one of the leading causes of death, of which myocardial infarction (MI) can cause irreversible damage and decrease survivors' quality of life, novel therapeutics are needed. Current approaches such as organ transplantation do not fully restore cardiac function or are limited. As a valuable strategy, tissue engineering seeks to obtain constructs that resemble myocardial tissue, vessels, and heart valves using cells, biomaterials as scaffolds, biochemical and physical stimuli. ...
Source: Bioprocess and Biosystems Engineering - April 20, 2024 Category: Biomedical Engineering Authors: Jorge Bravo-Ol ín Sabina A Mart ínez-Carreón Emmanuel Francisco-Solano Alvaro R Lara Nohra E Beltran-Vargas Source Type: research

Electricity generation and real oily wastewater treatment by Pseudomonas citronellolis 620C in a microbial fuel cell: pyocyanin production as electron shuttle
Bioprocess Biosyst Eng. 2024 Apr 17. doi: 10.1007/s00449-024-03016-1. Online ahead of print.ABSTRACTIn the present study, the potential of Pseudomonas citronellolis 620C strain was evaluated, for the first time, to generate electricity in a standard, double chamber microbial fuel cell (MFC), with oily wastewater (OW) being the fuel at 43.625 mg/L initial chemical oxygen demand (COD). Both electrochemical and physicochemical results suggested that this P. citronellolis strain utilized efficiently the OW substrate and generated electricity in the MFC setup reaching 0.05 mW/m2 maximum power. COD removal was remarkable reachin...
Source: Bioprocess and Biosystems Engineering - April 17, 2024 Category: Biomedical Engineering Authors: Constantina K Varnava Panagiotis Persianis Ioannis Ieropoulos Argyro Tsipa Source Type: research

Synthetic co-culture in an interconnected two-compartment bioreactor system: violacein production with recombinant E. coli strains
Bioprocess Biosyst Eng. 2024 Apr 16. doi: 10.1007/s00449-024-03008-1. Online ahead of print.ABSTRACTThe concept of modular synthetic co-cultures holds considerable potential for biomanufacturing, primarily to reduce the metabolic burden of individual strains by sharing tasks among consortium members. However, current consortia often show unilateral relationships solely, without stabilizing feedback control mechanisms, and are grown in a shared cultivation setting. Such 'one pot' approaches hardly install optimum growth and production conditions for the individual partners. Hence, novel mutualistic, self-coordinating consor...
Source: Bioprocess and Biosystems Engineering - April 16, 2024 Category: Biomedical Engineering Authors: Tobias M üller Simon Schick Jan-Simon Klemp Georg A Sprenger Ralf Takors Source Type: research

Evaluation of free and immobilized cellulase on chitosan-modified magnetic nanoparticles for saccharification of sorghum residue
Bioprocess Biosyst Eng. 2024 Apr 12. doi: 10.1007/s00449-024-03010-7. Online ahead of print.ABSTRACTEnzymatic hydrolysis plays a pivotal role in transforming lignocellulosic biomass. Addressing alternate techniques to optimize the utilization of cellulolytic enzymes is one strategy to improve its efficiency and lower process costs. Cellulases are highly specific and environmentally benign biocatalysts that break down intricate polysaccharides into simple forms of sugars. In contrast to the most difficult and time-consuming enzyme immobilization processes, in this research, we studied simple, mild, and successful techniques...
Source: Bioprocess and Biosystems Engineering - April 12, 2024 Category: Biomedical Engineering Authors: Pallavi Punia Lakhvinder Singh Source Type: research

Enhanced bactericidal, antibiofilm and antioxidative response of Lawsonia inermis leaf extract synthesized ZnO NPs loaded with commercial antibiotic
Bioprocess Biosyst Eng. 2024 Apr 12. doi: 10.1007/s00449-024-03000-9. Online ahead of print.ABSTRACTGlobally, antibiotic resistance is a challenging issue in healthcare sector. The emergence of multiple drug-resistant bacteria has forced us to modify existing medicines and or formulate newer medicines that are effective and inexpensive. In this perspective, this study involves the formation of zinc oxide nanoparticles (ZnO NPs) by utilizing the Lawsonia inermis (Li) leaf extract. The prepared L. inermis leaf extract mediated ZnO NPs (Li-ZnO NPs) were bio-physically characterized. The antibacterial and radical scavenging ef...
Source: Bioprocess and Biosystems Engineering - April 12, 2024 Category: Biomedical Engineering Authors: Balasubramanian Malaikozhundan Sonaimuthu Mohandoss Raman Krishnamoorthi Palanichamy Vidhya Bharathi Subramanian Palanisamy Jayaraj Vinodhini Source Type: research