Cofactor engineering in thermoanaerobacterium aotearoense SCUT27 for maximizing ethanol yield and revealing an enzyme complex with high ferredoxin-NAD < sup > + < /sup > reductase activity
Bioresour Technol. 2024 May 1:130784. doi: 10.1016/j.biortech.2024.130784. Online ahead of print.ABSTRACTThermoanaerobacterium aotearoense SCUT27 is a prominent producer of biofuels from lignocellulosic materials. To provide sufficient NAD(P)H for ethanol production, redox-related genes, including lactate dehydrogenase (ldh), redox-sensing transcriptional repressor (rex), and hydrogenase (hfsB), were knocked out. However, the growth of strain PRH (Δldh/Δrex/ΔhfsB) was suppressed due to the intracellular redox state imbalance with the increased NADH concentration. Coincidentally, when the Bcd-EtfAB (BCD) complex was over...
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Kaiqun Dai Chunyun Qu Xin Li Yang Lan Hongxin Fu Jufang Wang Source Type: research
Underwater light source changes microecosystem constructed by Vallisneria spinulosa Yan for water restoration: Efficiency of water purification, characteristics of growth, and rhizosphere
This study explored the influencing mechanism of illumination on Vallisneria spinulosa Yan (V. spinulosa Yan) for water restoration. Addition of underwater light source increased the total nitrogen, ammonia nitrogen, total phosphorus, and phosphate removal loads of the V. spinulosa Yan growth system by 61.5, 39.2, 8.5, and 5.0 mg m-2 d-1, respectively. Meanwhile, the growth of V. spinulosa Yan was obviously promoted, even with high water turbidity. Although the biological nitrogen removal processes were inhibited by adding underwater light source, the growth of V. spinulosa Yan can be significantly improved, thus enhancing...
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Wen Wang Jintao Zhang Min Zhao Chunzhen Fan Zhan Jin Ke Bei Zhiquan Wang Xiangyong Zheng Suqing Wu Source Type: research
Black soldier fly larvae bioconversion and subsequent composting promote larval frass quality during pig and chicken manure transformation process
Bioresour Technol. 2024 May 1:130777. doi: 10.1016/j.biortech.2024.130777. Online ahead of print.ABSTRACTThis research systematically assessed the changes in carbon, nitrogen and microbial profiling during pig and chicken manure transformation by black soldier fly larvae (BSFL) and subsequent composting process. BSFL had higher conversion efficiency for chicken manure. The pH, phosphorus and potassium contents in fresh BSFL frass increased than raw manure, but conductivity, total-/nitrate-/ammonium-nitrogen decreased. After BSFL conversion, pig manure had a larger nitrogen loss (25 %) while chicken manure had a larger carb...
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Nan Wu Ye Ma Xiaohui Yu Xiaobo Wang Qing Wang Xinyuan Liu Xiaoyan Xu Source Type: research
Coupling machine learning and theoretical models to compare key properties of biochar in adsorption kinetics rate and maximum adsorption capacity for emerging contaminants
Bioresour Technol. 2024 May 1:130776. doi: 10.1016/j.biortech.2024.130776. Online ahead of print.ABSTRACTInsights into key properties of biochar with a fast adsorption rate and high adsorption capacity are urgent to design biochar as an adsorbent in pollution emergency treatment. Machine learning (ML) incorporating classical theoretical adsorption models was applied to build prediction models for adsorption kinetics rate (i.e., K) and maximum adsorption capacity (i.e., Qm) of emerging contaminants (ECs) on biochar. Results demonstrated that the prediction performance of adaptive boosting algorithm significantly improved af...
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Bingyou Liu Feiyu Xi Huanjing Zhang Jiangtao Peng Lianpeng Sun Xinzhe Zhu Source Type: research
Effects of crayfish shell powder and bamboo-derived biochar on nitrogen conversion, bacterial community and nitrogen functional genes during pig manure composting
In conclusion, the results confirmed that TP3 was the most effective treatment in reducing nitrogen loss.PMID:38701980 | DOI:10.1016/j.biortech.2024.130783 (Source: Bioresource Technology)
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Zhuangzhuang Liu Shuhua Cao Xi He Gang Liu Hao Yao Sujuan Ding Jun Fang Source Type: research
Performance and microbial community composition of full-scale high-rate cascade sludge digestion system via pie-shaped reactor configuration
This study proves that the CAD system achieves stable sludge reduction, accelerates enzymatic hydrolysis at full-scale, and paves the way for its industrialization in municipal waste sewage sludge treatment.PMID:38701981 | DOI:10.1016/j.biortech.2024.130771 (Source: Bioresource Technology)
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Hongxiao Guo Maaike McIntyre Andre Visser Hans Kuipers Jules B van Lier Merle de Kreuk Source Type: research
Comprehensive review of microbial production of medium-chain fatty acids from waste activated sludge and enhancement strategy
Bioresour Technol. 2024 May 1:130782. doi: 10.1016/j.biortech.2024.130782. Online ahead of print.ABSTRACTMicrobial production of versatile applicability medium-chain fatty acids (MCFAs) (C6-C10) from waste activated sludge (WAS) provides a pioneering approach for wastewater treatment plants (WWTPs) to achieve carbon recovery. Mounting studies emerged endeavored to promote the MCFAs production from WAS while struggling with limited MCFAs production and selectivity. Herein, this review covers comprehensive introduction of the transformation process from WAS to MCFAs and elaborates the mechanisms for unsatisfactory MCFAs prod...
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Xin Gu Jing Sun Tong Wang Jia Li Han Wang Jialin Wang Yayi Wang Source Type: research
Systems metabolic engineering of Corynebacterium glutamicum to assimilate formic acid for biomass accumulation and succinic acid production
This study presents a breakthrough: engineered Corynebacterium glutamicum strains (L2-L4) through modular engineering. L2 incorporates the formate-tetrahydrofolate cycle and reverse glycine cleavage pathway, L3 enhances NAD(P)H regeneration, and L4 reinforces metabolic flux. Metabolic modeling elucidates C1 assimilation, guiding strain optimization for co-fermentation of formate and glucose. Strain L4 achieves an OD600 of 0.5 and produces 0.6 g/L succinic acid. 13C-labeled formate confirms C1 assimilation, and further laboratory evolution yields 1.3 g/L succinic acid. This study showcases a successful model for biologicall...
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Kai Li Xue Zhang Cheng Li Yu-Cheng Liang Xin-Qing Zhao Chen-Guang Liu Anthony J Sinskey Feng-Wu Bai Source Type: research
Improving anaerobic digestion performance after severe acidification: Unveiling the impacts of Fe < sub > 3 < /sub > O < sub > 4 < /sub > -bentonite composites in co-digestion of waste activated sludge and food waste
This study explored its in-situ recovery using a co-substrate of food waste and waste activated sludge. Fe3O4 and bentonite were used as conductor and carrier, respectively, to enhance AD performance under severe acidification. The application of Fe3O4-bentonite resulted in a 152% increase in cumulative methane in the Fe3O4-bentonite 10 digester, demonstrating its effectiveness in restoring the acidified AD system. In acidified systems, bentonite enhanced the diversity and richness of microbial communities due to its buffering capacity. The excessive non-conductive polysaccharides excreted by bacteria in extracellular poly...
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Ruilin Zhu Yongdong Chen Yangrui Huang Zhenzhen Tang Huaizheng Li Li Gu Source Type: research
Production of levulinic acid from macroalgae by hydrothermal conversion with ionic resin catalyst
This study focuses on the production of levulinic acid from G. verrucosa using hydrothermal conversion with an ionic resin Purolite CT269DR as the catalyst. By optimization of the conversion condition, a 30.3 % (22.58 g/L) yield of levulinic acid (LA) (based on carbohydrate content) was obtained at 200 °C for 90 min with 12.5 % biomass and 50 % catalyst loading of biomass quantity. Simultaneously, formic acid yielded 14.0 % (10.42 g/L). The LA yield increased with increasing combined severity (CS) levels under tested ranges. Furthermore, the relationship between CS and LA synthesis was effectively fitted to the nonlinear ...
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Youngshin Park Gwi-Taek Jeong Source Type: research
Sustainable biochar application in anammox process: Unveiling novel pathways for enhanced nitrogen removal and efficient start-up at low temperature
This study explored the use of biochar to accelerate the establishment of anaerobic ammonium oxidation (anammox) reactors operating at 15 ± 1℃. Incorporating 10 g/L bamboo charcoal in S1 accelerated the start-up of anammox in 87 days, which was significantly shorter than 103 days in S0 (without biochar). After 140 days, S1 exhibited a 10.9 % increase in nitrogen removal efficiency due to a 28.9 % elevation in extracellular polymeric substances, bolstering anammox bacterial resilience. Predominant anammox bacteria (Cadidatus Brocadia and Cadidatus Jettenia) showed relative abundances of 3.19 % and 0.38 % in S1, respectiv...
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Biao Yang Jiawei Sun Zhongyu Wang Yun Duan Source Type: research
Decisional tool development and application for techno-economic analysis of fungal laccase production
Bioresour Technol. 2024 May 1:130781. doi: 10.1016/j.biortech.2024.130781. Online ahead of print.ABSTRACTTextile and medical effluents causing bioaccumulation and biomagnification have been successfully biodegraded by fungal laccases. Here, a decision-making tool was developed and applied to evaluate 45 different laccase production strategies which determined the best potential source from a techno-economical perspective. Laccase production cost was calculated with a fixed output of 109 enzymatic units per batch (USD$per109U) and a sensitivity analysis was performed. Results indicate that optimization of enzymatic kinetics...
Source: Bioresource Technology - May 3, 2024 Category: Biotechnology Authors: Paola C Gutierrez-Rangel Karla Mayolo-Deloisa Mario A Torres-Acosta Source Type: research
Integration of anaerobic digestion and electrodialysis for methane yield promotion and in-situ ammonium recovery
This study proposed a novel approach, the electrodialysis-integrated AD (ADED) system, for in-situ recovery of ammonium (NH4+) while simultaneously enhancing AD performance. The ADED reactor was operated at two different NH4+-N concentrations (5,000 mg/L and 10,000 mg/L) to evaluate its performance against a conventional AD reactor. The results indicate that the ADED technology effectively reduced the NH4+-N concentration to below 2,000 mg/L, achieving this with a competitive energy consumption. Moreover, the ADED reactor demonstrated a 1.43-fold improvement in methane production when the influent NH4+-N was 5,000 mg/L, an...
Source: Bioresource Technology - May 2, 2024 Category: Biotechnology Authors: Jizhong Meng Lin Shi Yuansheng Hu Zhongzhong Wang Zhenhu Hu Xinmin Zhan Source Type: research
Analyses of long-term fungal degradation of spruce bark reveals varying potential for catabolism of polysaccharides and extractive compounds
Bioresour Technol. 2024 Apr 30:130768. doi: 10.1016/j.biortech.2024.130768. Online ahead of print.ABSTRACTThe bark represents the outer protective layer of trees. It contains high concentrations of antimicrobial extractives, in addition to regular wood polymers. It represents a huge underutilized side stream in forestry, but biotechnological valorization is hampered by a lack of knowledge on microbial bark degradation. Many fungi are efficient lignocellulose degraders, and here, spruce bark degradation by five species, Dichomitus squalens, Rhodonia placenta, Penicillium crustosum, Trichodermasp. B1, and Trichoderma reesei,...
Source: Bioresource Technology - May 2, 2024 Category: Biotechnology Authors: Amanda S Ristinmaa Ekaterina Korotkova Magnus Ø Arntzen Vincent G H Eijsink Chunlin Xu Anna Sundberg Merima Hasani Johan Larsbrink Source Type: research
Advancing the construction and application of genome-scale metabolic models for oleaginous microorganisms
Bioresour Technol. 2024 Apr 30:130706. doi: 10.1016/j.biortech.2024.130706. Online ahead of print.NO ABSTRACTPMID:38697368 | DOI:10.1016/j.biortech.2024.130706 (Source: Bioresource Technology)
Source: Bioresource Technology - May 2, 2024 Category: Biotechnology Authors: Chao Ye Source Type: research
