Fluorescent metal nanoclusters: From luminescence mechanism to applications in enzyme activity assays
Biosens Bioelectron. 2024 Apr 22;257:116323. doi: 10.1016/j.bios.2024.116323. Online ahead of print.ABSTRACTMetal nanoclusters (MNCs) have outstanding fluorescence property and biocompatibility, which show widespread applications in biological analysis. Particularly, evaluation of enzyme activity with the fluorescent MNCs has been developed rapidly within the past several years. In this review, we first introduced the fluorescent mechanism of mono- and bi-metallic nanoclusters, respectively, whose interesting luminescence properties are mainly resulted from electron transfer between the lowest unoccupied molecular orbital ...
Source: Biosensors and Bioelectronics - April 26, 2024 Category: Biotechnology Authors: Tong Wang Hong-Sheng Tan Ai-Jun Wang Shan-Shan Li Jiu-Ju Feng Source Type: research
An instrument-free, integrated micro-platform for rapid and multiplexed detection of dairy adulteration in resource-limited environments
In this study, we introduced a fully integrated and portable microfluidic detection platform called Sector Self-Driving Microfluidics (SDM), designed to simultaneously detect eight common species-specific components in milk. SDM integrated nucleic acid extraction, purification, loop-mediated isothermal amplification (LAMP), and lateral flow strip (LFS) detection functions into a closed microfluidic system, enabling contamination-free visual detection. The SDM platform used a constant-temperature heating plate, powered by a mobile battery, eliminated the need for additional power support. The SDM platform achieved nucleic a...
Source: Biosensors and Bioelectronics - April 26, 2024 Category: Biotechnology Authors: Nan Wang Xiaoyun Sun Juan Zhang Ying Chen Jiukai Zhang Fengchun Huang Ailiang Chen Source Type: research
MOF-derived sandwich-structured dual Z-Scheme Co < sub > 9 < /sub > S < sub > 8 < /sub > @ZnIn < sub > 2 < /sub > S < sub > 4 < /sub > /CdSe hollow nanocages heterojunction: Target-induced ultrasensitive photoelectrochemical sensing of chlorpyrifos
Biosens Bioelectron. 2024 Apr 22;257:116324. doi: 10.1016/j.bios.2024.116324. Online ahead of print.ABSTRACTExploring efficient photoactive material presents an intriguing opportunity to enhance the analytical performance of photoelectrochemical (PEC) sensor in the environmental analysis. In this work, a sandwich-structured multi-interface Co9S8@ZnIn2S4/CdSe QDs dual Z-Scheme heterojunction, derived from metal-organic framework (MOF), was synthesized as a sensing platform for chlorpyrifos detection, by integrating with enzyme-induced in situ insoluble precipitates strategy. The meticulously designed Co9S8@ZnIn2S4/CdSe QDs ...
Source: Biosensors and Bioelectronics - April 26, 2024 Category: Biotechnology Authors: Ben-Fang Xu Jin Zhang Aisyah Protonia Tanjung Fan Xu Ai-Jun Wang Li-Ping Mei Pei Song Jiu-Ju Feng Source Type: research
Electrospun EU/HPMC nanofibers decorated by ZIF-8 nanoparticle as the advanced electrochemical biosensor modifier for sensitive and selective detection of c-MET cancer biomarker in human plasma sample
Biosens Bioelectron. 2024 Apr 24;257:116319. doi: 10.1016/j.bios.2024.116319. Online ahead of print.ABSTRACTThis research presents a selective and sensitive electrochemical biosensor for the detection of the mesenchymal-epithelial transition factor (c-MET). The biosensing is based on a modification of the SPCE (screen-printed carbon electrode) with the electrospun nanofiber containing eudragit (EU), hydroxypropyl methylcellulose (HPMC), and Zeolite imidazolate frameworks (ZIF-8) nanoparticles. EU/HPMC/ZIF-8 nanofibers have presented a high capability of electron transfer, and more active surface area than bare SPCE due to ...
Source: Biosensors and Bioelectronics - April 26, 2024 Category: Biotechnology Authors: Fatemeh Maleki Habib Razmi Mohammad-Reza Rashidi Mehdi Yousefi Soghra Ramezani Marjan Ghorbani Source Type: research
Ultra-sensitive and rapid detection of Salmonella enterica and Staphylococcus aureus to single-cell level by aptamer-functionalized carbon nanotube field-effect transistor biosensors
Biosens Bioelectron. 2024 Apr 24;257:116333. doi: 10.1016/j.bios.2024.116333. Online ahead of print.ABSTRACTFoodborne diseases caused by Salmonella enterica (S. enterica) and Staphylococcus aureus (S. aureus) significantly impact public health, underscoring the imperative for highly sensitive, rapid, and accurate detection technologies to ensure food safety and prevent human diseases. Nanomaterials hold great promise in the development of high-sensitivity transistor biosensors. In this work, field-effect transistor (FET) comprising high-purity carbon nanotubes (CNTs) were fabricated and modified with corresponding nucleic ...
Source: Biosensors and Bioelectronics - April 26, 2024 Category: Biotechnology Authors: Xiaoxuan Feng Pengzhen Li Tingxian Li Xiaoling Cao Daohe Liu Mengmeng Xiao Li Wang Source Type: research
Combining deep learning and droplet microfluidics for rapid and label-free antimicrobial susceptibility testing of colistin
Biosens Bioelectron. 2024 Apr 16;257:116301. doi: 10.1016/j.bios.2024.116301. Online ahead of print.ABSTRACTEfficient tools for rapid antibiotic susceptibility testing (AST) are crucial for appropriate use of antibiotics, especially colistin, which is now often considered a last resort therapy with extremely drug resistant Gram-negative bacteria. Here, we developed a rapid, easy and miniaturized colistin susceptibility assay based on microfluidics, which allows for culture and high-throughput analysis of bacterial samples. Specifically, a simple microfluidic platform that can easily be operated was designed to encapsulate ...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Justine Riti Guillaume Sutra Thierry Naas Herv é Volland St éphanie Simon Karla Perez-Toralla Source Type: research
A cell-free biosensor for multiplexed and sensitive detection of biological warfare agents
This study introduces a cell-free biosensor for BWA detection by converting the 16S rRNA of targeted pathogens into detectable functional protein molecules. The modular nature of this approach allows for the flexible configuration of pathogen detection, enabling the simultaneous identification of multiple pathogenic 16S rRNAs through customized reporter proteins for each targeted sequence. Furthermore, we demonstrate how this method integrates with techniques utilizing retroreflective Janus particles (RJPs) for facile and highly sensitive pathogen detection. The cell-free biosensor, employing RJPs to measure the reflection...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Yu Jin Park Sunjoo Choi Kyung Won Lee So-Yoon Park Dong-Yeon Song Tae Hyeon Yoo Dong-Myung Kim Source Type: research
NIR-driven multifunctional PEC biosensor based on aptamer-modified PDA/MnO < sub > 2 < /sub > photoelectrode for bacterial detection and inactivation
Biosens Bioelectron. 2024 Apr 20;257:116320. doi: 10.1016/j.bios.2024.116320. Online ahead of print.ABSTRACTSensitive detection and effective inactivation of bacteria are essential in preventing foodborne bacterial infection that poses a significant threat to human health. Herein, a near-infrared (NIR)-driven multifunctional photoelectrochemical (PEC) biosensor was constructed for detection and inactivation of S. aureus. Based on the covalent bonding between amine and carboxyl groups, carboxyl-functionalized SA31 aptamer was immobilized on the PDA/MnO2 photoelectrode. In the presence of S. aureus, SA31 aptamer can specific...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Anni Cui Lihua Dong Yiting Hou Xin Mu Yu Sun Huan Wang Xiahua Zhong Guiye Shan Source Type: research
A dual-channel electrochemical biosensor enables concurrent detection of pathogens and antibiotic resistance
Biosens Bioelectron. 2024 Apr 20;257:116314. doi: 10.1016/j.bios.2024.116314. Online ahead of print.ABSTRACTDiarrheagenic E. coli infections, commonly treated with β-lactam antibiotics, contribute to antibiotic resistance - a pressing public health concern. Rapid monitoring of pathogen antibiotic resistance is vital to combat antimicrobial spread. Current bacterial diagnosis methods identify pathogens or determine antibiotic resistance separately, necessitating multiple assays. There is an urgent need for tools that simultaneously identify infectious agents and their antibiotic resistance at the point of care (POC). We de...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Dharanivasan Gunasekaran Irina Rostovsky David Taussig Talya Bar-Am Yariv Wine Neta Sal-Man Sefi Vernick Source Type: research
Vortexing-generated high throughput single-cell droplets for facile analysis of multiplexed microRNA dynamic secretion
Biosens Bioelectron. 2024 Apr 17;257:116303. doi: 10.1016/j.bios.2024.116303. Online ahead of print.ABSTRACTDiscriminating secretory phenotypes provides a direct, intact, and dynamic way to evaluate the heterogeneity in cell states and activation, which is significant for dissecting non-genetic heterogeneity for human health studies and disease diagnostics. In particular, secreted microRNAs, soluble signaling molecules released by various cells, are increasingly recognized as a critical mediator for cell-cell communication and the circulating biomarkers for disease diagnosis. However, single-cell analysis of secreted miRNA...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Fengjiao Zhu Yangyang Long Weiwei Shi Bin Li Yahui Ji Xue Bai Xianming Liu Dongyuan Qi Bo Sun Fuyin Zhang Tingjiao Liu Bingcheng Lin Yao Lu Source Type: research
Combining deep learning and droplet microfluidics for rapid and label-free antimicrobial susceptibility testing of colistin
Biosens Bioelectron. 2024 Apr 16;257:116301. doi: 10.1016/j.bios.2024.116301. Online ahead of print.ABSTRACTEfficient tools for rapid antibiotic susceptibility testing (AST) are crucial for appropriate use of antibiotics, especially colistin, which is now often considered a last resort therapy with extremely drug resistant Gram-negative bacteria. Here, we developed a rapid, easy and miniaturized colistin susceptibility assay based on microfluidics, which allows for culture and high-throughput analysis of bacterial samples. Specifically, a simple microfluidic platform that can easily be operated was designed to encapsulate ...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Justine Riti Guillaume Sutra Thierry Naas Herv é Volland St éphanie Simon Karla Perez-Toralla Source Type: research
A cell-free biosensor for multiplexed and sensitive detection of biological warfare agents
This study introduces a cell-free biosensor for BWA detection by converting the 16S rRNA of targeted pathogens into detectable functional protein molecules. The modular nature of this approach allows for the flexible configuration of pathogen detection, enabling the simultaneous identification of multiple pathogenic 16S rRNAs through customized reporter proteins for each targeted sequence. Furthermore, we demonstrate how this method integrates with techniques utilizing retroreflective Janus particles (RJPs) for facile and highly sensitive pathogen detection. The cell-free biosensor, employing RJPs to measure the reflection...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Yu Jin Park Sunjoo Choi Kyung Won Lee So-Yoon Park Dong-Yeon Song Tae Hyeon Yoo Dong-Myung Kim Source Type: research
NIR-driven multifunctional PEC biosensor based on aptamer-modified PDA/MnO < sub > 2 < /sub > photoelectrode for bacterial detection and inactivation
Biosens Bioelectron. 2024 Apr 20;257:116320. doi: 10.1016/j.bios.2024.116320. Online ahead of print.ABSTRACTSensitive detection and effective inactivation of bacteria are essential in preventing foodborne bacterial infection that poses a significant threat to human health. Herein, a near-infrared (NIR)-driven multifunctional photoelectrochemical (PEC) biosensor was constructed for detection and inactivation of S. aureus. Based on the covalent bonding between amine and carboxyl groups, carboxyl-functionalized SA31 aptamer was immobilized on the PDA/MnO2 photoelectrode. In the presence of S. aureus, SA31 aptamer can specific...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Anni Cui Lihua Dong Yiting Hou Xin Mu Yu Sun Huan Wang Xiahua Zhong Guiye Shan Source Type: research
A dual-channel electrochemical biosensor enables concurrent detection of pathogens and antibiotic resistance
Biosens Bioelectron. 2024 Apr 20;257:116314. doi: 10.1016/j.bios.2024.116314. Online ahead of print.ABSTRACTDiarrheagenic E. coli infections, commonly treated with β-lactam antibiotics, contribute to antibiotic resistance - a pressing public health concern. Rapid monitoring of pathogen antibiotic resistance is vital to combat antimicrobial spread. Current bacterial diagnosis methods identify pathogens or determine antibiotic resistance separately, necessitating multiple assays. There is an urgent need for tools that simultaneously identify infectious agents and their antibiotic resistance at the point of care (POC). We de...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Dharanivasan Gunasekaran Irina Rostovsky David Taussig Talya Bar-Am Yariv Wine Neta Sal-Man Sefi Vernick Source Type: research
Vortexing-generated high throughput single-cell droplets for facile analysis of multiplexed microRNA dynamic secretion
Biosens Bioelectron. 2024 Apr 17;257:116303. doi: 10.1016/j.bios.2024.116303. Online ahead of print.ABSTRACTDiscriminating secretory phenotypes provides a direct, intact, and dynamic way to evaluate the heterogeneity in cell states and activation, which is significant for dissecting non-genetic heterogeneity for human health studies and disease diagnostics. In particular, secreted microRNAs, soluble signaling molecules released by various cells, are increasingly recognized as a critical mediator for cell-cell communication and the circulating biomarkers for disease diagnosis. However, single-cell analysis of secreted miRNA...
Source: Biosensors and Bioelectronics - April 25, 2024 Category: Biotechnology Authors: Fengjiao Zhu Yangyang Long Weiwei Shi Bin Li Yahui Ji Xue Bai Xianming Liu Dongyuan Qi Bo Sun Fuyin Zhang Tingjiao Liu Bingcheng Lin Yao Lu Source Type: research
