'Gel-Stacks' gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study
Biotechniques. 2024 Apr 24. doi: 10.2144/btn-2023-0123. Online ahead of print.ABSTRACTLarge DNA molecules (>20 kb) are difficult analytes prone to breakage during serial manipulations and cannot be 'rescued' as full-length amplicons. Accordingly, to present, modify and analyze arrays of large, single DNA molecules, we created an easily realizable approach offering gentle confinement conditions or immobilization via spermidine condensation for controlled delivery of reagents that support live imaging by epifluorescence microscopy termed 'Gel-Stacks.' Molecules are locally confined between two hydrogel surfaces without co...
Source: BioTechniques - April 24, 2024 Category: Biotechnology Authors: Susana Calle-Caste ñeda Eamon Winden Alejandro Vasquez-Echeverri Matthew Schickling Evelyn Browning Juan Pablo Hernandez Ortiz David C Schwartz Source Type: research
'Gel-Stacks' gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study
Biotechniques. 2024 Apr 24. doi: 10.2144/btn-2023-0123. Online ahead of print.ABSTRACTLarge DNA molecules (>20 kb) are difficult analytes prone to breakage during serial manipulations and cannot be 'rescued' as full-length amplicons. Accordingly, to present, modify and analyze arrays of large, single DNA molecules, we created an easily realizable approach offering gentle confinement conditions or immobilization via spermidine condensation for controlled delivery of reagents that support live imaging by epifluorescence microscopy termed 'Gel-Stacks.' Molecules are locally confined between two hydrogel surfaces without co...
Source: BioTechniques - April 24, 2024 Category: Biotechnology Authors: Susana Calle-Caste ñeda Eamon Winden Alejandro Vasquez-Echeverri Matthew Schickling Evelyn Browning Juan Pablo Hernandez Ortiz David C Schwartz Source Type: research
'Gel-Stacks' gently confine or reversibly immobilize arrays of single DNA molecules for manipulation and study
Biotechniques. 2024 Apr 24. doi: 10.2144/btn-2023-0123. Online ahead of print.ABSTRACTLarge DNA molecules (>20 kb) are difficult analytes prone to breakage during serial manipulations and cannot be 'rescued' as full-length amplicons. Accordingly, to present, modify and analyze arrays of large, single DNA molecules, we created an easily realizable approach offering gentle confinement conditions or immobilization via spermidine condensation for controlled delivery of reagents that support live imaging by epifluorescence microscopy termed 'Gel-Stacks.' Molecules are locally confined between two hydrogel surfaces without co...
Source: BioTechniques - April 24, 2024 Category: Biotechnology Authors: Susana Calle-Caste ñeda Eamon Winden Alejandro Vasquez-Echeverri Matthew Schickling Evelyn Browning Juan Pablo Hernandez Ortiz David C Schwartz Source Type: research
Rectal swab DNA collection protocol for PCR genotyping in rats
This report evaluates the effects of collection parameters on the quality of PCR results and presents a protocol for genotyping a litter of rats within 3-5 h. Samples with 2-8 scrapes produced enough DNA to amplify targets up to ∼1800 bp long using PCR. Rectal swabbing produced PCR results with similar utility as ear clip samples, and results were unaffected by residual fecal matter or cell debris. The protocol enables fast, minimally invasive and repeatable genotyping using commercial PCR reagents.PMID:38652013 | DOI:10.2144/btn-2024-0023 (Source: BioTechniques)
Source: BioTechniques - April 23, 2024 Category: Biotechnology Authors: Audrey E Kaye Jacob W Proctor-Bonbright Jai Y Yu Source Type: research
Extraterrestrial agriculture: plant cultivation in space
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2024-0033. Online ahead of print.ABSTRACT[Formula: see text] Researchers are using various techniques and technologies to study how plants grow in extraterrestrial conditions with the hopes of sustaining longer missions for exploring deep space as well as being able to one day cultivate crops on other planets.PMID:38602376 | DOI:10.2144/btn-2024-0033 (Source: BioTechniques)
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Beatrice Bowlby Source Type: research
Development and evaluation of a single-tube nested PCR with colorimetric assay for < em > Mycobacterium tuberculosis < /em > detection
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2023-0080. Online ahead of print.ABSTRACTMolecular techniques have revolutionized tuberculosis (TB) diagnosis by offering a faster and more sensitive approach, detecting Mycobacterium tuberculosis (Mtb) DNA directly from samples. Single-tube nested PCR (STNPCR) combines two PCR reactions with separate oligonucleotide sets in a single tube. Moreover, colorimetric methods in PCR products have been studied for pathogen detection. Thus, this study aimed to establish a novel system based on colorimetric STNPCR for Mtb detection using microtiter plates with IS6110-amplified fragments....
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Marcela Pereira Salazar Juliana Figueiredo da Costa Lima Suassuna Monteiro Wlisses Henrique Veloso Carvalho-Silva George Tadeu Nunes Diniz Roberto Pereira Werkhauser L ílian Maria Lapa Montenegro Haiana Charifker Schindler Source Type: research
Extraterrestrial agriculture: plant cultivation in space
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2024-0033. Online ahead of print.ABSTRACT[Formula: see text] Researchers are using various techniques and technologies to study how plants grow in extraterrestrial conditions with the hopes of sustaining longer missions for exploring deep space as well as being able to one day cultivate crops on other planets.PMID:38602376 | DOI:10.2144/btn-2024-0033 (Source: BioTechniques)
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Beatrice Bowlby Source Type: research
Development and evaluation of a single-tube nested PCR with colorimetric assay for < em > Mycobacterium tuberculosis < /em > detection
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2023-0080. Online ahead of print.ABSTRACTMolecular techniques have revolutionized tuberculosis (TB) diagnosis by offering a faster and more sensitive approach, detecting Mycobacterium tuberculosis (Mtb) DNA directly from samples. Single-tube nested PCR (STNPCR) combines two PCR reactions with separate oligonucleotide sets in a single tube. Moreover, colorimetric methods in PCR products have been studied for pathogen detection. Thus, this study aimed to establish a novel system based on colorimetric STNPCR for Mtb detection using microtiter plates with IS6110-amplified fragments....
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Marcela Pereira Salazar Juliana Figueiredo da Costa Lima Suassuna Monteiro Wlisses Henrique Veloso Carvalho-Silva George Tadeu Nunes Diniz Roberto Pereira Werkhauser L ílian Maria Lapa Montenegro Haiana Charifker Schindler Source Type: research
Extraterrestrial agriculture: plant cultivation in space
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2024-0033. Online ahead of print.ABSTRACT[Formula: see text] Researchers are using various techniques and technologies to study how plants grow in extraterrestrial conditions with the hopes of sustaining longer missions for exploring deep space as well as being able to one day cultivate crops on other planets.PMID:38602376 | DOI:10.2144/btn-2024-0033 (Source: BioTechniques)
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Beatrice Bowlby Source Type: research
Development and evaluation of a single-tube nested PCR with colorimetric assay for < em > Mycobacterium tuberculosis < /em > detection
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2023-0080. Online ahead of print.ABSTRACTMolecular techniques have revolutionized tuberculosis (TB) diagnosis by offering a faster and more sensitive approach, detecting Mycobacterium tuberculosis (Mtb) DNA directly from samples. Single-tube nested PCR (STNPCR) combines two PCR reactions with separate oligonucleotide sets in a single tube. Moreover, colorimetric methods in PCR products have been studied for pathogen detection. Thus, this study aimed to establish a novel system based on colorimetric STNPCR for Mtb detection using microtiter plates with IS6110-amplified fragments....
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Marcela Pereira Salazar Juliana Figueiredo da Costa Lima Suassuna Monteiro Wlisses Henrique Veloso Carvalho-Silva George Tadeu Nunes Diniz Roberto Pereira Werkhauser L ílian Maria Lapa Montenegro Haiana Charifker Schindler Source Type: research
Extraterrestrial agriculture: plant cultivation in space
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2024-0033. Online ahead of print.ABSTRACT[Formula: see text] Researchers are using various techniques and technologies to study how plants grow in extraterrestrial conditions with the hopes of sustaining longer missions for exploring deep space as well as being able to one day cultivate crops on other planets.PMID:38602376 | DOI:10.2144/btn-2024-0033 (Source: BioTechniques)
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Beatrice Bowlby Source Type: research
Development and evaluation of a single-tube nested PCR with colorimetric assay for < em > Mycobacterium tuberculosis < /em > detection
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2023-0080. Online ahead of print.ABSTRACTMolecular techniques have revolutionized tuberculosis (TB) diagnosis by offering a faster and more sensitive approach, detecting Mycobacterium tuberculosis (Mtb) DNA directly from samples. Single-tube nested PCR (STNPCR) combines two PCR reactions with separate oligonucleotide sets in a single tube. Moreover, colorimetric methods in PCR products have been studied for pathogen detection. Thus, this study aimed to establish a novel system based on colorimetric STNPCR for Mtb detection using microtiter plates with IS6110-amplified fragments....
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Marcela Pereira Salazar Juliana Figueiredo da Costa Lima Suassuna Monteiro Wlisses Henrique Veloso Carvalho-Silva George Tadeu Nunes Diniz Roberto Pereira Werkhauser L ílian Maria Lapa Montenegro Haiana Charifker Schindler Source Type: research
Extraterrestrial agriculture: plant cultivation in space
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2024-0033. Online ahead of print.ABSTRACT[Formula: see text] Researchers are using various techniques and technologies to study how plants grow in extraterrestrial conditions with the hopes of sustaining longer missions for exploring deep space as well as being able to one day cultivate crops on other planets.PMID:38602376 | DOI:10.2144/btn-2024-0033 (Source: BioTechniques)
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Beatrice Bowlby Source Type: research
Development and evaluation of a single-tube nested PCR with colorimetric assay for < em > Mycobacterium tuberculosis < /em > detection
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2023-0080. Online ahead of print.ABSTRACTMolecular techniques have revolutionized tuberculosis (TB) diagnosis by offering a faster and more sensitive approach, detecting Mycobacterium tuberculosis (Mtb) DNA directly from samples. Single-tube nested PCR (STNPCR) combines two PCR reactions with separate oligonucleotide sets in a single tube. Moreover, colorimetric methods in PCR products have been studied for pathogen detection. Thus, this study aimed to establish a novel system based on colorimetric STNPCR for Mtb detection using microtiter plates with IS6110-amplified fragments....
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Marcela Pereira Salazar Juliana Figueiredo da Costa Lima Suassuna Monteiro Wlisses Henrique Veloso Carvalho-Silva George Tadeu Nunes Diniz Roberto Pereira Werkhauser L ílian Maria Lapa Montenegro Haiana Charifker Schindler Source Type: research
Extraterrestrial agriculture: plant cultivation in space
Biotechniques. 2024 Apr 11. doi: 10.2144/btn-2024-0033. Online ahead of print.ABSTRACT[Formula: see text] Researchers are using various techniques and technologies to study how plants grow in extraterrestrial conditions with the hopes of sustaining longer missions for exploring deep space as well as being able to one day cultivate crops on other planets.PMID:38602376 | DOI:10.2144/btn-2024-0033 (Source: BioTechniques)
Source: BioTechniques - April 11, 2024 Category: Biotechnology Authors: Beatrice Bowlby Source Type: research
