Biotechnology and Applied Biochemistry This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Synergistic effects of miR ‐143 with miR‐99a inhibited cell proliferation and induced apoptosis in breast cancer
In this study, the precursor structure of miRNAs in the expression vector pEGFP-N1 entered single and double states, and MCF7 and T47D cells were transfected. The miRNAs expression level after transfection was then measured using qPCR. The MultiMiR package was used to obtain predicted and validated miRNA targets. MTT assay, qRT-PCR, migration test, and flow cytometry were used to assess the effect of miRNA and gene modulation. The qPCR results revealed that miRNA constructs were significantly expressed after the transfection of both cell lines. The biological function of miRNAs showed that upregulation of miR-99a and miR-1...
Source: Biotechnology and Applied Biochemistry - May 1, 2024 Category: Biochemistry Authors: Zahra Doosti, Syed Omar Ebrahimi, Mahshid Samie Ghahfarokhi, Somayeh Reiisi Tags: ORIGINAL ARTICLE Source Type: research

Quantitative detection of mpox antigen using time ‐resolved fluorescence immunochromatography
This study aims to establish a time-resolved fluorescence immunochromatography (TRFICO) method for qualitative/quantitative detection of mpox virus. A double-antibody sandwich TRFICO method was optimized and established using mpox recombinant fusion antigen and its paired monoclonal antibody. The test performance of the method was evaluated using mpox fusion antigen and control serum, including sensitivity, linearity range, specificity, precision, and reference interval. We successfully established a TRFICO method for qualitative/quantitative detection of mpox antigen, its linearity range 0 –100 ng/mL, analytical sensit...
Source: Biotechnology and Applied Biochemistry - May 1, 2024 Category: Biochemistry Authors: Huankun Liang, Cuicui Chen, Tiancai Liu, Wenqi Dong, Laiqing Li Tags: ORIGINAL ARTICLE Source Type: research

Purification and characterization of glutamate dehydrogenase from rainbow trout (Oncorhynchus mykiss) liver and molecular docking studies
In this study, GDH enzyme was purified from the liver of rainbow trout (Oncorhynchus mykiss) by 2',5'-ADP Sepharose 4B affinity chromatography in one step. As a result of this purification process, GDH enzyme was purified 171-fold with 5.83  U/mg protein-specific activity. The characterization experiments presented that the storage stability of the purified GDH enzyme was determined as −80°C; optimum temperature 40°C; it was determined that the optimum ionic strength was 100 mM phosphate buffer and the optimum pH was 8.00. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and PAGE studies showed th...
Source: Biotechnology and Applied Biochemistry - May 1, 2024 Category: Biochemistry Authors: Onur Ertik, Refiye Yanardag Tags: ORIGINAL ARTICLE Source Type: research

Investigation of the effects of thiazole compounds on thioredoxin reductase 1 (TrxR1), glutathione S ‐transferase (GST), and glutathione reductase (GR) targeted human brain glioblastoma cancer (U‐87 MG)
AbstractCancer is a fatal disease that kills thousands of people worldwide. Despite the information produced by research on cancer treatment, applications in cancer treatment are limited. Therefore, scientists ’ efforts to develop more effective treatment approaches continue. In the study, we aimed to determine the anticancer potential of amino thiazole compounds on human glioblastoma (U-87 MG) and human dermal fibroblast (HDFa) cells and their inhibition effects on enzymes that cause multidrug resistan ce in cancer cells. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide cell viability test was performed ...
Source: Biotechnology and Applied Biochemistry - April 30, 2024 Category: Biochemistry Authors: I şıl Nihan Korkmaz Tags: ORIGINAL ARTICLE Source Type: research

In vitro antibacterial activity of antibiotics and plant essential oils against Escherichia coli MTCC443 supported through the molecular docking and pharmacokinetics study
This study has been made to compare the in vitro antibacterial activity and further validated the same through the molecular docking study of 13 antibiotics such as ciprofloxacin, chloramphenicol, erythromycin, ampicillin, cefotaxime, rifampicin, kanamycin, vancomycin, streptomycin, penicillin, nalidixic acid, trimethoprim, and polymyxin, and 10 EOs such as garlic, tulsi, neem, clove, thyme, peppermint, coriander, tea, lavender, and eucalyptus against the target protein (DNA gyrase) ofE. coli MTCC443.E. coli Microbial Type Culture Collection 443 was found to be highly sensitive to ciprofloxacin (zone of inhibition [ZOI], 2...
Source: Biotechnology and Applied Biochemistry - April 17, 2024 Category: Biochemistry Authors: Saurabh Jain, Adarsh Kumar Shukla, Surbhi Panwar, Rajesh Kumar, Ashwani Kumar Tags: ORIGINAL ARTICLE Source Type: research

Characterization and validation of fatty acid metabolism ‐related genes predicting prognosis, immune infiltration, and drug sensitivity in endometrial cancer
AbstractEndometrial cancer is considered to be the second most common tumor of the female reproductive system, and patients diagnosed with advanced endometrial cancer have a poor prognosis. The influence of fatty acid metabolism in the prognosis of patients with endometrial cancer remains unclear. We constructed a prognostic risk model using transcriptome sequencing data of endometrial cancer and clinical information of patients from The Cancer Genome Atlas (TCGA) database via least absolute shrinkage and selection operator regression analysis. The tumor immune microenvironment was analyzed using the CIBERSORT algorithm, f...
Source: Biotechnology and Applied Biochemistry - April 15, 2024 Category: Biochemistry Authors: Haojia Li, Ting Zhou, Qi Zhang, Yuwei Yao, Teng Hua, Jun Zhang, Hongbo Wang Tags: ORIGINAL ARTICLE Source Type: research

Sensitive osteoarthritis sensing by salt ‐induced aggregation and dispersion of gold nanoparticles
AbstractOsteoarthritis occurs in any joints, and identification in its earlier stages helps to treat the disease and increase the recovery rate. The radiography method and imaging techniques are traditionally used to identify osteoarthritis. But these methods are expensive, and with the complicated steps. Researchers are working toward developing a highly sensitive biosensor in identifying the osteoarthritis biomarker. This research was focused on developing a C-terminal telopeptide of type II collagen (CTX-II) colorimetric sensor with gold nanoparticle (AuNP) for diagnosing osteoarthritis. Anti-CTX-II was conjugated with ...
Source: Biotechnology and Applied Biochemistry - April 13, 2024 Category: Biochemistry Authors: Yong Fan, Renjie Li, Xuekai Deng, Ming Li, Yu Zhou, Dan Wu, Gang Liu Tags: ORIGINAL ARTICLE Source Type: research

The effects of hyaluronan and proteoglycan link protein 1 (HAPLN1) in ameliorating spinal cord injury mediated by Nrf2
In this study, both in vitro and in vivo SCI models were established. Nissl staining and terminal deoxynucleotidyl transferase dUTP nick end labeling staining assays were used. Western blotting and enzyme-linked immunosorbent assay were employed to assess the expression of proteins. Our results demonstrate that the administration of HAPLN1 promoted the recovery of motor neurons after SCI by increasing the Basso mouse scale score, increasing the numbers of motor neurons, and preventing apoptosis of spinal cord cells. Additionally, HAPLN1 mitigated OS in spinal cord tissue after SCI by increasing the content of superoxide di...
Source: Biotechnology and Applied Biochemistry - April 12, 2024 Category: Biochemistry Authors: Hongzhi Yang, Bin Hu, Xichun Wang, Wenjie Chen, Huanbin Zhou Tags: ORIGINAL ARTICLE Source Type: research

Issue Information
(Source: Biotechnology and Applied Biochemistry)
Source: Biotechnology and Applied Biochemistry - April 10, 2024 Category: Biochemistry Tags: ISSUE INFORMATION Source Type: research

Facile construction of gefitinib ‐loaded zeolitic imidazolate framework nanocomposites for the treatment of different lung cancer cells
AbstractGefitinib (GET) is a revolutionary targeted treatment inhibiting the epidermal growth factor receptor's tyrosine kinase action by competitively inhibiting the ATP binding site. In preclinical trials, several lung cancer cell lines and xenografts have demonstrated potential activity with GET. Response rates neared 25% in preclinical trials for non-small cell lung cancer. Here, we describe the one-pot synthesis of GET@ZIF-8 nanocomposites (NCs) in pure water, encapsulating zeolitic imidazolate framework 8 (ZIF-8). This method developed NCs with consistent morphology and a loading efficiency of 9%, resulting in a load...
Source: Biotechnology and Applied Biochemistry - April 10, 2024 Category: Biochemistry Authors: Kalaivani Aiyasamy, Malathi Ramasamy, Abdurahman Hajinur Hirad, Palanisamy Arulselvan, Ravindran Jaganathan, Jagadeesh Suriyaprakash, Indumathi Thangavelu, Abdullah A. Alarfaj Tags: ORIGINAL ARTICLE Source Type: research

Overview of cloning in lactic acid bacteria: Expression and its application of probiotic potential in inflammatory bowel diseases
AbstractInflammatory bowel disease (IBD) imposes a significant impact on the quality of life for affected individuals. However, there was a current lack of a systematic summary regarding the latest epidemic trends and the underlying pathogenesis of IBD. This highlights the need for a thorough examination of both the epidemiological aspects of IBD and the specific mechanisms by which lactic acid bacteria (LAB) contribute to mitigating this condition. In developed countries, higher incidences and death rates of IBD have been observed, influenced by a combination of environmental and genetic factors. LAB offer significant adv...
Source: Biotechnology and Applied Biochemistry - April 5, 2024 Category: Biochemistry Authors: K. E. Vivekanandan, R. Kasimani, P. Vinoth Kumar, S. Meenatchisundaram, William Arputha Prabhu Tags: REVIEW ARTICLE Source Type: research

CB ‐0821, a novel CC chemokine receptor 5 (CCR5) inhibitor with improved binding efficacy proposed as anti‐HIV candidate: Computational and in vitro approach
In this study, CB-0821, identified from the ChemBridge library, emerged as a promising CCR5 inhibitor. Molecular dynamics simulations indicate comparable dynamic properties for CB-0821 and MVC. In silico comparisons with other CCR5 inhibitors emphasize CB-0821's superior binding affinity, positioning it as a potential lead compound. Evaluations of the dissociation constant (Ki) and absorption, distribution, metabolism, and excretion predictions suggest CB-0821 as a well-tolerated drug. Furthermore, the dose-dependent inhibition of CCR5 by CB-0821 in Peripheral blood mononuclear cells (PBMCs) (ranging from 10 to 200  nM) d...
Source: Biotechnology and Applied Biochemistry - April 3, 2024 Category: Biochemistry Authors: Ashish Kumar Tags: ORIGINAL ARTICLE Source Type: research

Microsatellite instability evaluation by a novel PCR ‐based 8‐loci test kit in colorectal cancer
This study evaluated the reliability and accuracy of a novel polymerase chain reaction (PCR)-based 8-loci MSI test kit, a rapid test kit designed to detect MSI, by comparing its performance with immunohistochemistry (IHC) and the National Cancer Institute (NCI) 2B3D Panel. MSI status was determined in 186 formalin-fixed paraffin-embedded (FFPE) colorectal cancer tissue samples with known mismatch repair (MMR) status by IHC using the novel PCR-based 8-loci MSI test kit. Additionally, the consistency between the NCI 2B3D Panel and the novel PCR-based 8-loci panel was compared using 69 FFPE tumor tissues paired with adjacent ...
Source: Biotechnology and Applied Biochemistry - April 1, 2024 Category: Biochemistry Authors: Gaofang Xiao, Jing Li, Lijun Deng, Shuangquan Gao, Caiyun Tan, Guiqing He, Richang Du Tags: ORIGINAL ARTICLE Source Type: research