Deinococcus wulumuqiensis R12 synthesized silver nanoparticles with peroxidase-like activity for synergistic antibacterial application
Biotechnol J. 2024 Apr;19(4):e2300584. doi: 10.1002/biot.202300584.ABSTRACTThe use of a combination of several antibacterial agents for therapy holds great promise in reducing the dosage and side effects of these agents, improving their efficiency, and inducing potential synergistic therapeutic effects. Herein, this study provides an innovative antibacterial treatment strategy by synergistically combining R12-AgNPs with H2O2 therapy. R12-AgNPs were simply produced with the supernatant of an ionizing radiation-tolerant bacterium Deinococcus wulumuqiensis R12 by one-step under room temperature. In comparison with chemically ...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Jingjia Liu Nan Zhang Bowen Shen Liling Zhang Zhidong Zhang Liying Zhu Ling Jiang Source Type: research
Tuning CpG motif position in nanostructured DNA for efficient immune stimulation
Biotechnol J. 2024 Apr;19(4):e2300308. doi: 10.1002/biot.202300308.ABSTRACTIt was previously demonstrated that polypod-like nanostructured DNA (polypodna) comprising three or more oligodeoxynucleotides (ODNs) were useful for the delivery of ODNs containing cytosine-phosphate-guanine (CpG) motifs, or CpG ODNs, to immune cells. Although the immunostimulatory activity of single-stranded CpG ODNs is highly dependent on CpG motif sequence and position, little is known about how the position of the motif affects the immunostimulatory activity of CpG motif-containing nanostructured DNAs. In the present study, four series of polyp...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Mengmeng Tan Natsuki Makiguchi Kosuke Kusamori Shoko Itakura Yuki Takahashi Yoshinobu Takakura Makiya Nishikawa Source Type: research
Overexpression of YTHDF3 increases the specific productivity of the recombinant protein in CHO cells by promoting the translation process
In conclusion, the findings in this study provide a novel approach for mammalian cell engineering to increase protein productivity by regulating m6A.PMID:38651251 | DOI:10.1002/biot.202400078 (Source: Biotechnology Journal)
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Zhao-Ming Cui Ying-Ying Feng Yan-Ping Gao Hai-Tong Wang Jiang-Tao Lu Jia-Liang Guo Hong-Yan Xu Le-le Qiu Tian-Yun Wang Yan-Long Jia Source Type: research
Phase separation methods for protein purification: A meta-analysis of purification performance and cost-effectiveness
Biotechnol J. 2024 Apr;19(4):e2400005. doi: 10.1002/biot.202400005.ABSTRACTProtein purifications based on phase separations (e.g., precipitation and liquid-liquid extraction) have seen little adoption in commercial protein drug production. To identify barriers, we analyzed the purification performance and economics of 290 phase separation purifications from 168 publications. First, we found that studies using Design of Experiments for optimization achieved significantly greater mean yield and host cell protein log10 removal values than those optimizing one factor at a time (11.5% and 53% increases, respectively). Second, b...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: John S Decker Utsuki Yano Romel Menacho Melgar Michael D Lynch Source Type: research
Biological autoluminescence enables effective monitoring of yeast cell electroporation
Biotechnol J. 2024 Apr;19(4):e2300475. doi: 10.1002/biot.202300475.ABSTRACTThe application of pulsed electric fields (PEFs) is becoming a promising tool for application in biotechnology, and the food industry. However, real-time monitoring of the efficiency of PEF treatment conditions is challenging, especially at the industrial scale and in continuous production conditions. To overcome this challenge, we have developed a straightforward setup capable of real-time detection of yeast biological autoluminescence (BAL) during pulsing. Saccharomyces cerevisiae culture was exposed to 8 pulses of 100 µs width with electric fiel...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Martin Bereta Michal Teplan Tom áš Zakar Hoang Vuviet Michal Cifra Djamel Eddine Chafai Source Type: research
Accelerated generation of gene-engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9
Biotechnol J. 2024 Apr;19(4):e2300505. doi: 10.1002/biot.202300505.ABSTRACTChinese hamster ovary (CHO) cells are the commonly used mammalian host system to manufacture recombinant proteins including monoclonal antibodies. However unfavorable non-human glycoprofile displayed on CHO-produced monoclonal antibodies have negative impacts on product quality, pharmacokinetics, and therapeutic efficiency. Glycoengineering such as genetic elimination of genes involved in glycosylation pathway in CHO cells is a viable solution but constrained due to longer timeline and laborious workflow. Here, in this proof-of-concept (PoC) study, ...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Justin S Antony Anabel Migenda Herranz Tahereh Mohammadian Gol Susanne Mailand Paul Monnier Jennifer Rottenberger Alicia Roig-Merino Bettina Keller Claudia Gowin Maria Milla Tobias A Beyer Markus Mezger Source Type: research
Development of an efficient liposomal DOX delivery formulation for HCC therapy by targeting CK2 α
Biotechnol J. 2024 Apr;19(4):e2400050. doi: 10.1002/biot.202400050.ABSTRACTHepatocellular carcinoma (HCC) is a digestive tract cancer with high mortality and poor prognosis, especially in China. Current chemotherapeutic drugs lead to poor prognosis, low efficacy, and high side effects due to weak targeting specificity and rapidly formed multidrug resistance (MDR). Based on the previous studies on the doxorubicin (DOX) formulation for cancer targeting therapy, we developed a novel DOX delivery formulation for the targeting chemotherapy of HCC and DOX resistant HCC. HCSP4 was previously screened and casein kinase 2α (CK2α)...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Ruixia Zhao Sinan Cheng Xue Bai Danying Zhang Hongming Fang Wanlin Che Wenxuan Zhang Yujuan Zhou Wei Duan Qiumin Liang Li Xiao Guochao Nie Yingchun Hou Source Type: research
Deinococcus wulumuqiensis R12 synthesized silver nanoparticles with peroxidase-like activity for synergistic antibacterial application
Biotechnol J. 2024 Apr;19(4):e2300584. doi: 10.1002/biot.202300584.ABSTRACTThe use of a combination of several antibacterial agents for therapy holds great promise in reducing the dosage and side effects of these agents, improving their efficiency, and inducing potential synergistic therapeutic effects. Herein, this study provides an innovative antibacterial treatment strategy by synergistically combining R12-AgNPs with H2O2 therapy. R12-AgNPs were simply produced with the supernatant of an ionizing radiation-tolerant bacterium Deinococcus wulumuqiensis R12 by one-step under room temperature. In comparison with chemically ...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Jingjia Liu Nan Zhang Bowen Shen Liling Zhang Zhidong Zhang Liying Zhu Ling Jiang Source Type: research
Tuning CpG motif position in nanostructured DNA for efficient immune stimulation
Biotechnol J. 2024 Apr;19(4):e2300308. doi: 10.1002/biot.202300308.ABSTRACTIt was previously demonstrated that polypod-like nanostructured DNA (polypodna) comprising three or more oligodeoxynucleotides (ODNs) were useful for the delivery of ODNs containing cytosine-phosphate-guanine (CpG) motifs, or CpG ODNs, to immune cells. Although the immunostimulatory activity of single-stranded CpG ODNs is highly dependent on CpG motif sequence and position, little is known about how the position of the motif affects the immunostimulatory activity of CpG motif-containing nanostructured DNAs. In the present study, four series of polyp...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Mengmeng Tan Natsuki Makiguchi Kosuke Kusamori Shoko Itakura Yuki Takahashi Yoshinobu Takakura Makiya Nishikawa Source Type: research
Overexpression of YTHDF3 increases the specific productivity of the recombinant protein in CHO cells by promoting the translation process
In conclusion, the findings in this study provide a novel approach for mammalian cell engineering to increase protein productivity by regulating m6A.PMID:38651251 | DOI:10.1002/biot.202400078 (Source: Biotechnology Journal)
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Zhao-Ming Cui Ying-Ying Feng Yan-Ping Gao Hai-Tong Wang Jiang-Tao Lu Jia-Liang Guo Hong-Yan Xu Le-le Qiu Tian-Yun Wang Yan-Long Jia Source Type: research
Phase separation methods for protein purification: A meta-analysis of purification performance and cost-effectiveness
Biotechnol J. 2024 Apr;19(4):e2400005. doi: 10.1002/biot.202400005.ABSTRACTProtein purifications based on phase separations (e.g., precipitation and liquid-liquid extraction) have seen little adoption in commercial protein drug production. To identify barriers, we analyzed the purification performance and economics of 290 phase separation purifications from 168 publications. First, we found that studies using Design of Experiments for optimization achieved significantly greater mean yield and host cell protein log10 removal values than those optimizing one factor at a time (11.5% and 53% increases, respectively). Second, b...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: John S Decker Utsuki Yano Romel Menacho Melgar Michael D Lynch Source Type: research
Biological autoluminescence enables effective monitoring of yeast cell electroporation
Biotechnol J. 2024 Apr;19(4):e2300475. doi: 10.1002/biot.202300475.ABSTRACTThe application of pulsed electric fields (PEFs) is becoming a promising tool for application in biotechnology, and the food industry. However, real-time monitoring of the efficiency of PEF treatment conditions is challenging, especially at the industrial scale and in continuous production conditions. To overcome this challenge, we have developed a straightforward setup capable of real-time detection of yeast biological autoluminescence (BAL) during pulsing. Saccharomyces cerevisiae culture was exposed to 8 pulses of 100 µs width with electric fiel...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Martin Bereta Michal Teplan Tom áš Zakar Hoang Vuviet Michal Cifra Djamel Eddine Chafai Source Type: research
Accelerated generation of gene-engineered monoclonal CHO cell lines using FluidFM nanoinjection and CRISPR/Cas9
Biotechnol J. 2024 Apr;19(4):e2300505. doi: 10.1002/biot.202300505.ABSTRACTChinese hamster ovary (CHO) cells are the commonly used mammalian host system to manufacture recombinant proteins including monoclonal antibodies. However unfavorable non-human glycoprofile displayed on CHO-produced monoclonal antibodies have negative impacts on product quality, pharmacokinetics, and therapeutic efficiency. Glycoengineering such as genetic elimination of genes involved in glycosylation pathway in CHO cells is a viable solution but constrained due to longer timeline and laborious workflow. Here, in this proof-of-concept (PoC) study, ...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Justin S Antony Anabel Migenda Herranz Tahereh Mohammadian Gol Susanne Mailand Paul Monnier Jennifer Rottenberger Alicia Roig-Merino Bettina Keller Claudia Gowin Maria Milla Tobias A Beyer Markus Mezger Source Type: research
Development of an efficient liposomal DOX delivery formulation for HCC therapy by targeting CK2 α
Biotechnol J. 2024 Apr;19(4):e2400050. doi: 10.1002/biot.202400050.ABSTRACTHepatocellular carcinoma (HCC) is a digestive tract cancer with high mortality and poor prognosis, especially in China. Current chemotherapeutic drugs lead to poor prognosis, low efficacy, and high side effects due to weak targeting specificity and rapidly formed multidrug resistance (MDR). Based on the previous studies on the doxorubicin (DOX) formulation for cancer targeting therapy, we developed a novel DOX delivery formulation for the targeting chemotherapy of HCC and DOX resistant HCC. HCSP4 was previously screened and casein kinase 2α (CK2α)...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Ruixia Zhao Sinan Cheng Xue Bai Danying Zhang Hongming Fang Wanlin Che Wenxuan Zhang Yujuan Zhou Wei Duan Qiumin Liang Li Xiao Guochao Nie Yingchun Hou Source Type: research
Deinococcus wulumuqiensis R12 synthesized silver nanoparticles with peroxidase-like activity for synergistic antibacterial application
Biotechnol J. 2024 Apr;19(4):e2300584. doi: 10.1002/biot.202300584.ABSTRACTThe use of a combination of several antibacterial agents for therapy holds great promise in reducing the dosage and side effects of these agents, improving their efficiency, and inducing potential synergistic therapeutic effects. Herein, this study provides an innovative antibacterial treatment strategy by synergistically combining R12-AgNPs with H2O2 therapy. R12-AgNPs were simply produced with the supernatant of an ionizing radiation-tolerant bacterium Deinococcus wulumuqiensis R12 by one-step under room temperature. In comparison with chemically ...
Source: Biotechnology Journal - April 23, 2024 Category: Biotechnology Authors: Jingjia Liu Nan Zhang Bowen Shen Liling Zhang Zhidong Zhang Liying Zhu Ling Jiang Source Type: research
