Mutation-guided vaccine design: A process for developing boosting immunogens for HIV broadly neutralizing antibody induction
Cell Host Microbe. 2024 Apr 22:S1931-3128(24)00126-4. doi: 10.1016/j.chom.2024.04.006. Online ahead of print.ABSTRACTA major goal of HIV-1 vaccine development is the induction of broadly neutralizing antibodies (bnAbs). Although success has been achieved in initiating bnAb B cell lineages, design of boosting immunogens that select for bnAb B cell receptors with improbable mutations required for bnAb affinity maturation remains difficult. Here, we demonstrate a process for designing boosting immunogens for a V3-glycan bnAb B cell lineage. The immunogens induced affinity-matured antibodies by selecting for functional improba...
Source: Cell Host and Microbe - April 26, 2024 Category: Microbiology Authors: Kevin Wiehe Kevin O Saunders Victoria Stalls Derek W Cain Sravani Venkatayogi Joshua S Martin Beem Madison Berry Tyler Evangelous Rory Henderson Bhavna Hora Shi-Mao Xia Chuancang Jiang Amanda Newman Cindy Bowman Xiaozhi Lu Mary E Bryan Joena Bal Aja Sanzo Source Type: research
Mutation-guided vaccine design: A process for developing boosting immunogens for HIV broadly neutralizing antibody induction
Cell Host Microbe. 2024 Apr 22:S1931-3128(24)00126-4. doi: 10.1016/j.chom.2024.04.006. Online ahead of print.ABSTRACTA major goal of HIV-1 vaccine development is the induction of broadly neutralizing antibodies (bnAbs). Although success has been achieved in initiating bnAb B cell lineages, design of boosting immunogens that select for bnAb B cell receptors with improbable mutations required for bnAb affinity maturation remains difficult. Here, we demonstrate a process for designing boosting immunogens for a V3-glycan bnAb B cell lineage. The immunogens induced affinity-matured antibodies by selecting for functional improba...
Source: Cell Host and Microbe - April 26, 2024 Category: Microbiology Authors: Kevin Wiehe Kevin O Saunders Victoria Stalls Derek W Cain Sravani Venkatayogi Joshua S Martin Beem Madison Berry Tyler Evangelous Rory Henderson Bhavna Hora Shi-Mao Xia Chuancang Jiang Amanda Newman Cindy Bowman Xiaozhi Lu Mary E Bryan Joena Bal Aja Sanzo Source Type: research
Mutation-guided vaccine design: A process for developing boosting immunogens for HIV broadly neutralizing antibody induction
Cell Host Microbe. 2024 Apr 22:S1931-3128(24)00126-4. doi: 10.1016/j.chom.2024.04.006. Online ahead of print.ABSTRACTA major goal of HIV-1 vaccine development is the induction of broadly neutralizing antibodies (bnAbs). Although success has been achieved in initiating bnAb B cell lineages, design of boosting immunogens that select for bnAb B cell receptors with improbable mutations required for bnAb affinity maturation remains difficult. Here, we demonstrate a process for designing boosting immunogens for a V3-glycan bnAb B cell lineage. The immunogens induced affinity-matured antibodies by selecting for functional improba...
Source: Cell Host and Microbe - April 26, 2024 Category: Microbiology Authors: Kevin Wiehe Kevin O Saunders Victoria Stalls Derek W Cain Sravani Venkatayogi Joshua S Martin Beem Madison Berry Tyler Evangelous Rory Henderson Bhavna Hora Shi-Mao Xia Chuancang Jiang Amanda Newman Cindy Bowman Xiaozhi Lu Mary E Bryan Joena Bal Aja Sanzo Source Type: research
Mutation-guided vaccine design: A process for developing boosting immunogens for HIV broadly neutralizing antibody induction
Cell Host Microbe. 2024 Apr 22:S1931-3128(24)00126-4. doi: 10.1016/j.chom.2024.04.006. Online ahead of print.ABSTRACTA major goal of HIV-1 vaccine development is the induction of broadly neutralizing antibodies (bnAbs). Although success has been achieved in initiating bnAb B cell lineages, design of boosting immunogens that select for bnAb B cell receptors with improbable mutations required for bnAb affinity maturation remains difficult. Here, we demonstrate a process for designing boosting immunogens for a V3-glycan bnAb B cell lineage. The immunogens induced affinity-matured antibodies by selecting for functional improba...
Source: Cell Host and Microbe - April 26, 2024 Category: Microbiology Authors: Kevin Wiehe Kevin O Saunders Victoria Stalls Derek W Cain Sravani Venkatayogi Joshua S Martin Beem Madison Berry Tyler Evangelous Rory Henderson Bhavna Hora Shi-Mao Xia Chuancang Jiang Amanda Newman Cindy Bowman Xiaozhi Lu Mary E Bryan Joena Bal Aja Sanzo Source Type: research
The gut microbiome associates with phenotypic manifestations of post-acute COVID-19 syndrome
This study demonstrates that the gut microbiome is associated with phenotypic manifestations of PACS, which has potential clinical utility for the prediction and diagnosis of PACS.PMID:38657605 | DOI:10.1016/j.chom.2024.04.005 (Source: Cell Host and Microbe)
Source: Cell Host and Microbe - April 24, 2024 Category: Microbiology Authors: Qi Su Raphaela I Lau Qin Liu Moses K T Li Joyce Wing Yan Mak Wenqi Lu Ivan S F Lau Louis H S Lau Giann T Y Yeung Chun Pan Cheung Whitney Tang Chengyu Liu Jessica Y L Ching Pui Kuan Cheong Francis K L Chan Siew C Ng Source Type: research
Microbiota metabolism of intestinal amino acids impacts host nutrient homeostasis and physiology
Cell Host Microbe. 2024 Apr 22:S1931-3128(24)00121-5. doi: 10.1016/j.chom.2024.04.004. Online ahead of print.ABSTRACTThe intestine and liver are thought to metabolize dietary nutrients and regulate host nutrient homeostasis. Here, we find that the gut microbiota also reshapes the host amino acid (aa) landscape via efficiently metabolizing intestinal aa. To identify the responsible microbes/genes, we developed a metabolomics-based assay to screen 104 commensals and identified candidates that efficiently utilize aa. Using genetics, we identified multiple responsible metabolic genes in phylogenetically diverse microbes. By co...
Source: Cell Host and Microbe - April 24, 2024 Category: Microbiology Authors: Ting-Ting Li Xi Chen Da Huo Mohammad Arifuzzaman Shanshan Qiao Wen-Bing Jin Huiqing Shi Xin V Li JRI Live Cell Bank Consortium Iliyan D Iliev David Artis Chun-Jun Guo Source Type: research
A phase-separated protein hub modulates resistance to Fusarium head blight in wheat
Cell Host Microbe. 2024 Apr 16:S1931-3128(24)00119-7. doi: 10.1016/j.chom.2024.04.002. Online ahead of print.ABSTRACTFusarium head blight (FHB) is a devastating wheat disease. Fhb1, the most widely applied genetic locus for FHB resistance, is conferred by TaHRC of an unknown mode of action. Here, we show that TaHRC alleles distinctly drive liquid-liquid phase separation (LLPS) within a proteinaceous complex, determining FHB susceptibility or resistance. TaHRC-S (susceptible) exhibits stronger LLPS ability than TaHRC-R (resistant), and this distinction is further intensified by fungal mycotoxin deoxynivalenol, leading to op...
Source: Cell Host and Microbe - April 24, 2024 Category: Microbiology Authors: Yi He Xiujuan Yang Xiaobo Xia Yuhua Wang Yifan Dong Lei Wu Peng Jiang Xu Zhang Cong Jiang Hongxiang Ma Wujun Ma Cong Liu Ryan Whitford Matthew R Tucker Zhengguang Zhang Gang Li Source Type: research
The gut microbiome associates with phenotypic manifestations of post-acute COVID-19 syndrome
This study demonstrates that the gut microbiome is associated with phenotypic manifestations of PACS, which has potential clinical utility for the prediction and diagnosis of PACS.PMID:38657605 | DOI:10.1016/j.chom.2024.04.005 (Source: Cell Host and Microbe)
Source: Cell Host and Microbe - April 24, 2024 Category: Microbiology Authors: Qi Su Raphaela I Lau Qin Liu Moses K T Li Joyce Wing Yan Mak Wenqi Lu Ivan S F Lau Louis H S Lau Giann T Y Yeung Chun Pan Cheung Whitney Tang Chengyu Liu Jessica Y L Ching Pui Kuan Cheong Francis K L Chan Siew C Ng Source Type: research
Microbiota metabolism of intestinal amino acids impacts host nutrient homeostasis and physiology
Cell Host Microbe. 2024 Apr 22:S1931-3128(24)00121-5. doi: 10.1016/j.chom.2024.04.004. Online ahead of print.ABSTRACTThe intestine and liver are thought to metabolize dietary nutrients and regulate host nutrient homeostasis. Here, we find that the gut microbiota also reshapes the host amino acid (aa) landscape via efficiently metabolizing intestinal aa. To identify the responsible microbes/genes, we developed a metabolomics-based assay to screen 104 commensals and identified candidates that efficiently utilize aa. Using genetics, we identified multiple responsible metabolic genes in phylogenetically diverse microbes. By co...
Source: Cell Host and Microbe - April 24, 2024 Category: Microbiology Authors: Ting-Ting Li Xi Chen Da Huo Mohammad Arifuzzaman Shanshan Qiao Wen-Bing Jin Huiqing Shi Xin V Li JRI Live Cell Bank Consortium Iliyan D Iliev David Artis Chun-Jun Guo Source Type: research
A phase-separated protein hub modulates resistance to Fusarium head blight in wheat
Cell Host Microbe. 2024 Apr 16:S1931-3128(24)00119-7. doi: 10.1016/j.chom.2024.04.002. Online ahead of print.ABSTRACTFusarium head blight (FHB) is a devastating wheat disease. Fhb1, the most widely applied genetic locus for FHB resistance, is conferred by TaHRC of an unknown mode of action. Here, we show that TaHRC alleles distinctly drive liquid-liquid phase separation (LLPS) within a proteinaceous complex, determining FHB susceptibility or resistance. TaHRC-S (susceptible) exhibits stronger LLPS ability than TaHRC-R (resistant), and this distinction is further intensified by fungal mycotoxin deoxynivalenol, leading to op...
Source: Cell Host and Microbe - April 24, 2024 Category: Microbiology Authors: Yi He Xiujuan Yang Xiaobo Xia Yuhua Wang Yifan Dong Lei Wu Peng Jiang Xu Zhang Cong Jiang Hongxiang Ma Wujun Ma Cong Liu Ryan Whitford Matthew R Tucker Zhengguang Zhang Gang Li Source Type: research
Assessing phage-host population dynamics by reintroducing virulent viruses to synthetic microbiomes
Cell Host Microbe. 2024 Apr 18:S1931-3128(24)00114-8. doi: 10.1016/j.chom.2024.04.001. Online ahead of print.ABSTRACTMicrobiomes feature complex interactions between diverse bacteria and bacteriophages. Synthetic microbiomes offer a powerful way to study these interactions; however, a major challenge is obtaining a representative bacteriophage population during the bacterial isolation process. We demonstrate that colony isolation reliably excludes virulent viruses from sample sources with low virion-to-bacteria ratios such as feces, creating "virulent virus-free" controls. When the virulent dsDNA virome is reintroduced to ...
Source: Cell Host and Microbe - April 23, 2024 Category: Microbiology Authors: Jacob Wilde Randy Boyes Avery V Robinson Brendan A Daisley Alexander J Botschner Dylan J L Brettingham Christine V Macpherson Elizabeth Mallory Emma Allen-Vercoe Source Type: research
The complete Kaposi sarcoma-associated herpesvirus genome induces early-onset, metastatic angiosarcoma in transgenic mice
Cell Host Microbe. 2024 Apr 15:S1931-3128(24)00112-4. doi: 10.1016/j.chom.2024.03.012. Online ahead of print.ABSTRACTKaposi sarcoma (KS) is the most common cancer in persons living with HIV. It is caused by KS-associated herpesvirus (KSHV). There exists no animal model for KS. Pronuclear injection of the 170,000-bp viral genome induces early-onset, aggressive angiosarcoma in transgenic mice. The tumors are histopathologically indistinguishable from human KS. As in human KS, all tumor cells express the viral latency-associated nuclear antigen (LANA). The tumors transcribe most viral genes, whereas endothelial cells in other...
Source: Cell Host and Microbe - April 23, 2024 Category: Microbiology Authors: Sang-Hoon Sin Anthony B Eason Yongbaek Kim Johann W Schneider Blossom Damania Dirk P Dittmer Source Type: research
Assessing phage-host population dynamics by reintroducing virulent viruses to synthetic microbiomes
Cell Host Microbe. 2024 Apr 18:S1931-3128(24)00114-8. doi: 10.1016/j.chom.2024.04.001. Online ahead of print.ABSTRACTMicrobiomes feature complex interactions between diverse bacteria and bacteriophages. Synthetic microbiomes offer a powerful way to study these interactions; however, a major challenge is obtaining a representative bacteriophage population during the bacterial isolation process. We demonstrate that colony isolation reliably excludes virulent viruses from sample sources with low virion-to-bacteria ratios such as feces, creating "virulent virus-free" controls. When the virulent dsDNA virome is reintroduced to ...
Source: Cell Host and Microbe - April 23, 2024 Category: Microbiology Authors: Jacob Wilde Randy Boyes Avery V Robinson Brendan A Daisley Alexander J Botschner Dylan J L Brettingham Christine V Macpherson Elizabeth Mallory Emma Allen-Vercoe Source Type: research
The complete Kaposi sarcoma-associated herpesvirus genome induces early-onset, metastatic angiosarcoma in transgenic mice
Cell Host Microbe. 2024 Apr 15:S1931-3128(24)00112-4. doi: 10.1016/j.chom.2024.03.012. Online ahead of print.ABSTRACTKaposi sarcoma (KS) is the most common cancer in persons living with HIV. It is caused by KS-associated herpesvirus (KSHV). There exists no animal model for KS. Pronuclear injection of the 170,000-bp viral genome induces early-onset, aggressive angiosarcoma in transgenic mice. The tumors are histopathologically indistinguishable from human KS. As in human KS, all tumor cells express the viral latency-associated nuclear antigen (LANA). The tumors transcribe most viral genes, whereas endothelial cells in other...
Source: Cell Host and Microbe - April 23, 2024 Category: Microbiology Authors: Sang-Hoon Sin Anthony B Eason Yongbaek Kim Johann W Schneider Blossom Damania Dirk P Dittmer Source Type: research
Burkholderia thailandensis uses a type VI secretion system to lyse protrusions without triggering host cell responses
Cell Host Microbe. 2024 Apr 16:S1931-3128(24)00113-6. doi: 10.1016/j.chom.2024.03.013. Online ahead of print.ABSTRACTTo spread within a host, intracellular Burkholderia form actin tails to generate membrane protrusions into neighboring host cells and use type VI secretion system-5 (T6SS-5) to induce cell-cell fusions. Here, we show that B. thailandensis also uses T6SS-5 to lyse protrusions to directly spread from cell to cell. Dynamin-2 recruitment to the membrane near a bacterium was followed by a short burst of T6SS-5 activity. This resulted in the polymerization of the actin of the newly invaded host cell and disruption...
Source: Cell Host and Microbe - April 19, 2024 Category: Microbiology Authors: Miro Thorsten Wilhelm Plum Hoi Ching Cheung Patricia Reist Iscar Yahua Chen Yunn-Hwen Gan Marek Basler Source Type: research
