Nirmatrelvir/ritonavir treatment of patients with COVID-19 taking tacrolimus: case series describing the results of drug-drug interactions
We describe the cases of two patients treated with nirmatrelvir/ritonavir: a patient who had undergone kidney transplantation and another with a history of hematopoietic stem cell transplantation. Toxic concentrations of tacrolimus were induced in both. This case series highlights the risk associated with the concomitant administration of tacrolimus and nirmatrelvir/ritonavir.PMID:38698526 | DOI:10.1177/03000605241247705 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Dan Shen Yinhua Gong Yulan Qian Jianguo Zhu Jie Gao Source Type: research
Umbilical Cord Blood and UC-MSCs Combined with Low-Dose Immunosuppressant in the Treatment of Elderly Patients with Pure Red Cell Aplastic: A Case Series
CONCLUSION: This report provides an effective regimen of using UCB and UC-MSCs combined with low-dose CsA (1-3 mg/kg/d) to treat PRCA, especially for elderly patients with multiple complications who cannot use the conventional dosage.PMID:38698745 | DOI:10.2174/011574888X290378240424075002 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Wei-Wei Zhu Sujing Zhuang Zhe Yu Xin Li Tian-Jie Han Yue Ma Li-Jun Li Zhi-Rui Zhao Source Type: research
Single-Cell Proteomics Accelerates toward Proteoforms
J Proteome Res. 2024 May 3;23(5):1545-1546. doi: 10.1021/acs.jproteome.4c00290.NO ABSTRACTPMID:38698756 | DOI:10.1021/acs.jproteome.4c00290 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Sarah N Sipe Nikolai Slavov Source Type: research
The Prognosis for Delayed Immune Recovery in HIV-Infected Children might be Associated with Pre-cART CD4 + T cell Count Irrespective of Co-Infection with Tuberculosis
Res Sq [Preprint]. 2024 Apr 19:rs.3.rs-4243586. doi: 10.21203/rs.3.rs-4243586/v1.ABSTRACTBackground : Immune reconstitution following the initiation of combination antiretroviral therapy (cART) significantly impacts the prognosis of individuals infected with human immunodeficiency virus (HIV). Our previous studies have indicated that the baseline CD4 + T cells count and percentage before cART initiation are predictors of immune recovery in TB-negative children infected with HIV, with TB co-infection potentially causing a delay in immune recovery. However, it remains unclear whether these predictors consistently impact immu...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Funsho Ogunshola Ruhul Khan Musie Ghebremichael Source Type: research
Homeostasis of cytoplasmic crowding by cell wall fluidization and ribosomal counterions
Res Sq [Preprint]. 2024 Apr 19:rs.3.rs-4138690. doi: 10.21203/rs.3.rs-4138690/v1.ABSTRACTIn bacteria, algae, fungi, and plant cells, the wall must expand in concert with cytoplasmic biomass production, otherwise cells would experience toxic molecular crowding 1,2 or lyse. But how cells achieve expansion of this complex biomaterial in coordination with biosynthesis of macromolecules in the cytoplasm remains unexplained 3 , although recent works have revealed that these processes are indeed coupled 4,5 . Here, we report a striking increase of turgor pressure with growth rate in E. coli , suggesting that the speed of cell wal...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Markus Basan Avik Mukherjee Yanqing Huang Seungeun Oh Carlos Sanchez Yu-Fang Chang Xili Liu Gary Bradshaw Nina Benites Johan Paulsson Marc Kirschner Yongjin Sung Jens Elgeti Source Type: research
Single-molecule RNA-FISH analysis reveals stochasticity in reactivation of latent HIV-1 regulated by Nuclear Orphan Receptors NR4A and cMYC
Res Sq [Preprint]. 2024 Apr 19:rs.3.rs-4166090. doi: 10.21203/rs.3.rs-4166090/v1.ABSTRACTHIV-1 eradication strategies require complete reactivation of HIV-1 latent cells by Latency Reversing Agents (LRA). Current methods lack effectiveness due to incomplete proviral reactivation. We employed a single-molecule RNA-FISH (smRNA-FISH) and FISH-Quant analysis and found that proviral reactivation is highly variable from cell-to-cell, stochastic, and occurs in bursts and waves, with different kinetics in response to diverse LRAs. Approximately 1-5% of latent cells exhibited stochastic reactivation without LRAs. Through single-cel...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Annalena LaPorte Rajiv Pathak Carolina Eliscovich Laura Martins Rachel Nell Adam Spivak Masako Suzuki Vicente Planelles Robert Singer Ganjam Kalpana Source Type: research
Voriconazole Metabolism is Associated with the Number of Skin Cancers Per Patient
Res Sq [Preprint]. 2024 Apr 19:rs.3.rs-4152279. doi: 10.21203/rs.3.rs-4152279/v1.ABSTRACTVoriconazole exposure is associated with skin cancer, but it is unknown how the full spectrum of its metabolizer phenotypes impacts this association. We conducted a retrospective cohort study to determine how variation in metabolism of voriconazole as measured by metabolizer status of CYP2C19 is associated with the total number of skin cancers a patient develops and the rate of development of the first skin cancer after treatment. There were 1,739 organ transplant recipients with data on CYP2C19 phenotype. Of these, 134 were exposed to...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Jacqueline I Ike Isabelle T Smith Dominique Mosley Christopher Madden Sarah Grossarth Briana R Halle Adam Lewis Frank Mentch Hakon Hakonarson Lisa Bastarache Lee Wheless Source Type: research
Supinoxin blocks Small Cell Lung Cancer Progression by Inhibiting Mitochondrial Respiration through the RNA Helicase DDX5
Res Sq [Preprint]. 2024 Apr 15:rs.3.rs-4169007. doi: 10.21203/rs.3.rs-4169007/v1.ABSTRACTDDX5 is a DEAD-box RNA helicase that is overexpressed and implicated in progression of several cancers 1-4. One of these is small cell lung cancer (SCLC). Our laboratory has demonstrated that the RNA helicase DDX5 is essential for the invasive growth of SCLC and mitochondrial respiration 5. SCLC is an extremely lethal, recalcitrant tumor 6,7, causing 250,000 deaths annually worldwide 8 and currently lacking effective treatments 9,10. Supinoxin (RX 5902), a compound having anti-cancer activity 11, is a known target of phosphor-DDX5 12,1...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Elizabeth Tran Subhadeep Das Matthew Russon Maria Zea Zheng Xing Sandra Torregrosa-Allen Heidi Cervantes Bennett Elzey Haley Harper Source Type: research
Characterization of a Fatty Acid Amide Hydrolase (FAAH) in Hirudo verbana
In this study, we report on the identification and characterization of a fatty acid aminohydrolase (FAAH) in the medicinal leech, Hirudo verbana . FAAH is the primary enzyme responsible for metabolizing the endocannabinoid signaling molecule arachidonoyl ethanolamide (anandamide or AEA) and therefore plays a critical role in regulating AEA levels in the nervous system. This Hirudo FAAH (HirFAAH) is expressed in the leech central nervous system (CNS) and is an orthologue of FAAH-2 observed in vertebrates. Functionally, HirFAAH has serine hydrolase activity based on activity-based protein profiling (ABPP) studies using the f...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Emily Kabeiseman Riley T Paulsen Brian D Burrell Source Type: research
Mesoscale Metabolic Channeling Revealed by Multimodal Microscopy
Res Sq [Preprint]. 2024 Apr 17:rs.3.rs-4096781. doi: 10.21203/rs.3.rs-4096781/v1.ABSTRACTMetabolic homeostasis within cells and tissues requires engagement of catabolic and anabolic pathways consuming nutrients needed to generate energy to drive these and other subcellular processes. However, the current understanding of cell homeostasis and metabolism, including how cells utilize nutrients, comes largely from tissue and cell models analyzed after fractionation. These bulk strategies do not reveal the spatial characteristics of cell metabolism at the single cell level, and how these aspects relate to the location of cells ...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Rafael Arrojo E Drigo Aliyah Habashy Christopher Acree Keun-Young Kim Thomas Deerinck Emilee Patterson Louise Lantier Owen McGuinness Mark Ellisman Source Type: research
Coagulation and platelet biology at the intersection of health and disease: illustrated capsules of the 11th Symposium on Hemostasis at the University of North Carolina
Res Pract Thromb Haemost. 2024 Apr 3;8(3):102395. doi: 10.1016/j.rpth.2024.102395. eCollection 2024 Mar.ABSTRACTThe University of North Carolina Symposia on Hemostasis began in 2002, with The First Symposium on Hemostasis with a Special Focus on FVIIa and Tissue Factor. They have occurred biannually since and have maintained the primary goal of establishing a forum for the sharing of outstanding advances made in the basic sciences of hemostasis. The 2024 11th Symposium on Hemostasis will bring together leading scientists from around the globe to present and discuss the latest research related to coagulation factors and pla...
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Maria Aleman Gowthami M Arepally Trevor Baglin Lorena Buitrago Pavel Davizon-Castillo Sanjana Dayal Matthew J Flick Gloria Gerber Yohei Hisada Krasimir Kolev Ana O'Loghlen Alireza R Rezaie Erica M Sparkenbaugh Evi X Stavrou Bet ül Ünlü Gregory M Vercel Source Type: research
Regulation of Microtubule Stability in Pulmonary Microvascular Endothelial Cells in Rats with Severe Acute Pancreatitis: Qingyi Decoction is a Potential CDK5 Inhibitor
CONCLUSION: CDK5 has been identified as a critical target for pulmonary endothelial injury of SAP-ALI. QYD may partially alleviate microtubule disassembly by targeting the CDK5/NFAT5/GEF-H1 signaling pathway, thus relieving SAP-induced pulmonary microvascular endothelial cell injury.PMID:38699595 | PMC:PMC11063490 | DOI:10.2147/JIR.S451755 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Yinan Cao Fan Li Zhenxuan Sun Jin Liu Jie Liu Qi Yang Peng Ge Yalan Luo Hailong Chen Source Type: research
Investigation of Trpa1 and Trpc1 Immunreactivities in Colon Adenocarcinomas
CONCLUSION: TRPA1 and TRPC1 immunoreactivites are increased in colorectal adenocarcinoma tissue compared with the healthy tissue. Furthermore, the immunoreactivity decreases as the grade of cancer increases.PMID:38699653 | PMC:PMC11063473 | DOI:10.2147/CMAR.S447549 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Ahmet Bozdag Tuncay Kuloglu Gokhan Artas Suleyman Aydin Source Type: research
Fracture haematoma proteomics
CONCLUSION: The early fxH proteome of the ETC group was characterized by the expression of immunomodulatory, mainly pro-inflammatory, proteins, whereas the early fxH proteome of the DCO group was more regenerative and osteogenic in nature. These findings match clinical observations, in which enhanced surgical trauma after multiple trauma causes dysbalanced inflammation, potentially leading to reduced tissue regeneration, and gained insights into regulatory mechanisms of fracture healing after severe trauma.PMID:38699779 | DOI:10.1302/2046-3758.135.BJR-2023-0323.R1 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Rald V M Groven Christel Kuik Johannes Greven Ümit Mert Freek G Bouwman Martijn Poeze Taco J Blokhuis Markus Huber-Lang Frank Hildebrand Berta Cillero-Pastor Martijn van Griensven Source Type: research
Vaspin facilitates the proliferation and osteogenic differentiation of periodontal ligament stem cells
CONCLUSIONS: In summary, vaspin observably reduces HG-mediated inhibition of PDLSCs OD by modulating the TGF-β1/Smad signaling pathway. Vaspin may be a potential therapeutic for periodontal tissue regeneration in diabetic patients.PMID:38699861 | DOI:10.1111/jre.13254 (Source: Cell Research)
Source: Cell Research - May 3, 2024 Category: Cytology Authors: Zhihui Jing Xinran Feng Xin Li Xiaoyu Zhang Chunling Pan Source Type: research
