Infectious complications in pediatric patients undergoing CD19+CD22+ chimeric antigen receptor T-cell therapy for relapsed/refractory B-lymphoblastic leukemia
This study retrospectively analyzed infectious complications in 79 pediatric patients with R/R B-ALL treated with CAR-T cells at our institution. Overall, 53 patients developed 88 infections. Nine patients experienced nine infections during lymphodepletion chemotherapy, 35 experienced 41 infections during the early phase (days 0 –+ 30 after infusion), and 29 experienced 38 infections during the late phase (day + 31–+ 90 after infusion). Pathogens were identified in 31 infections, including 23 bacteria, seven viruses, and one fungus. Four patients were admitted to the intensive care unit for infection and one di...
Source: Clinical and Experimental Medicine - April 25, 2024 Category: Research Source Type: research
Extrachromosomal circular DNA (eccDNA): from carcinogenesis to drug resistance
AbstractExtrachromosomal circular DNA (eccDNA) is a circular form of DNA that exists outside of the chromosome. Although it has only been a few decades since its discovery, in recent years, it has been found to have a close relationship with cancer, which has attracted widespread attention from researchers. Thus far, under the persistent research of researchers from all over the world, eccDNA has been found to play an important role in a variety of tumors, including breast cancer, lung cancer, ovarian cancer, etc. Herein, we review the sources of eccDNA, classifications, and the mechanisms responsible for their biogenesis....
Source: Clinical and Experimental Medicine - April 25, 2024 Category: Research Source Type: research
A novel diagnostic model based on lncRNA PTPRE expression, neutrophil count and red blood cell distribution width for diagnosis of seronegative rheumatoid arthritis
AbstractDiagnosis of seronegative rheumatoid arthritis (SNRA) is difficult due to the lack of diagnostic markers. The study aims to construct a novel diagnostic model based on long noncoding RNAs (lncRNAs) expression and laboratory indicators to provide a new idea for diagnostic methods of SNRA. Differentially expressed lncRNAs in peripheral blood cells of RA patients were screened through eukaryotic long noncoding RNA sequencing and validated by quantitative real-time PCR. Meanwhile, the correlation between lncRNAs expression and laboratory indicators was analyzed. The diagnostic value was evaluated by receiver operating ...
Source: Clinical and Experimental Medicine - April 25, 2024 Category: Research Source Type: research
miR-17-92a-1 cluster host gene: a key regulator in colorectal cancer development and progression
AbstractColorectal cancer (CRC), recognized among the five most prevalent malignancies and most deadly cancers, manifests multifactorial influences stemming from environmental exposures, dietary patterns, age, and genetic predisposition. Although substantial progress has been made in comprehending the etiology of CRC, the precise genetic components driving its pathogenesis remain incompletely elucidated. Within the expansive repertoire of non-coding RNAs, particular focus has centered on the miR-17-92a-1 cluster host gene (MIR17HG) and its associated miRNAs, which actively participate in diverse cellular processes and freq...
Source: Clinical and Experimental Medicine - April 25, 2024 Category: Research Source Type: research
Fibroblast expression of neurotransmitter receptor HTR2A associates with inflammation in rheumatoid arthritis joint
AbstractThe study of neuroimmune crosstalk and the involvement of neurotransmitters in inflammation and bone health has illustrated their significance in joint-related conditions. One important mode of cell-to-cell communication in the synovial fluid (SF) is through extracellular vesicles (EVs) carrying microRNAs (miRNAs). The role of neurotransmitter receptors in the pathogenesis of inflammatory joint diseases, and whether there are specific miRNAs regulating differentially expressed HTR2A, contributing to the inflammatory processes and bone metabolism is unclear. Expression of neurotransmitter receptors and their corre...
Source: Clinical and Experimental Medicine - April 25, 2024 Category: Research Source Type: research
Improving quality of life after breast cancer: a comparison of two microsurgical treatment options for breast cancer-related lymphedema (BCRL)
ConclusionsBoth VLNT and LVA offer significant improvement for patients suffering from chronic BCRL. VLNT shows an even greater potential for improvement in more severe cases of BCRL, but involves a higher risk for (mostly minor) complications. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - April 23, 2024 Category: Research Source Type: research
Does the SARS-CoV-2 mRNA vaccine and its serum IgG levels affect fertility treatments and obstetric outcomes? An observational cohort study
ConclusionWe cautiously state that the COVID-19 mRNA vaccine does not affect fertility outcomes, including fertilization, pregnancy and delivery rates, obstetric outcomes, and semen parameters, regardless of measured IgG levels. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - April 23, 2024 Category: Research Source Type: research
Genomic characterization and detection of potential therapeutic targets for peritoneal mesothelioma in current practice
AbstractPeritoneal mesothelioma (PeM) is an aggressive tumor with limited treatment options. The current study aimed to evaluate the value of next generation sequencing (NGS) of PeM samples in current practice. Foundation Medicine F1CDx NGS was performed on 20 tumor samples. This platform assesses 360 commonly somatically mutated genes in solid tumors and provides a genomic signature. Based on the detected mutations, potentially effective targeted therapies were identified. NGS was successful in 19 cases. Tumor mutational burden (TMB) was low in 10 cases, and 11 cases were microsatellite stable. In the other cases, TMB and...
Source: Clinical and Experimental Medicine - April 20, 2024 Category: Research Source Type: research
Exploring natural killer cell-related biomarkers in multiple myeloma: a novel nature killer cell-related model predicting prognosis and immunotherapy response using single-cell study
ConclusionA new prognostic model for MM associated with NKs was constructed and validated. This model provides a fresh perspective for predicting patient outcomes, immunotherapeutic response, and candidate drugs. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - April 18, 2024 Category: Research Source Type: research
Comparison of trace elements in peripheral blood and bone marrow of newly diagnosed multiple myeloma patients
In this study, we aimed to evaluate alterations in trace elements in the blood and bone marrow serum of patients with newly diagnosed multiple myeloma (NMM). The levels of zinc (Zn), copper (Cu), iron (Fe), manganese (Mn), magnesium (Mg), selenium (Se), arsenic (As), boron (B), nickel (Ni), silicon (Si) and chromium (Cr) were analyzed in the venous blood samples of the patient group comprising 70 patients with NMM (41 males and 29 females) and compared to those in the control group comprising 30 individuals (18 males and 12 females). In addition, trace element levels were analyzed in bone marrow samples from the patient gr...
Source: Clinical and Experimental Medicine - April 17, 2024 Category: Research Source Type: research
Clinical impact of transient lymphopenia
In this study, 240 patients (142 men; 98 female; mean age 75.1 ± 15.1) were enrolled. Patients were divided into two groups according to the lymphocytes count at hospital admission, namely “Group A” with lymphopenia and “Group B” with values in the normal range. Moreover, the patients in group A were distinguished in relation to the regression or persistence of the lymphopenia assessed at the time of hospital discharge (Group A1: persistence; Group A2: normalization). Prevalence of lymphopenia at admission was 57%; Group A showed higher mean age and percentage of patients over 65 years of age; and none diffe...
Source: Clinical and Experimental Medicine - April 17, 2024 Category: Research Source Type: research
Genetic alterations and allele frequency of BRAF V600E and TERT mutation in papillary thyroid carcinoma with intermediate-to-high recurrence risk: a retrospective study
AbstractThe predictive value of allele frequency (AF) ofBRAF V600E andTERT mutations in papillary thyroid carcinoma (PTC) remains controversial. We aimed to investigate theAF ofBRAF V600E andTERT mutations in intermediate-to-high risk PTC and their association between tumor invasiveness, prognosis, and other mutations. Probe hybridization capture and high-throughput sequencing were used to quantitatively test 40 gene loci in 94 intermediate-to-high recurrence risk PTC patients, combined with clinical characteristics and follow-up for retrospective analysis.BRAF V600E mutationAF was linked to a increased risk of thyroid cap...
Source: Clinical and Experimental Medicine - April 12, 2024 Category: Research Source Type: research
A MiR181/Sirtuin1 regulatory circuit modulates drug response in biliary cancers
AbstractDespite recent advances, biliary tract cancer (BTC) remains one of the most lethal tumor worldwide due to late diagnosis, limited therapeutic strategies and resistance to conventional therapies. In recent years, high-throughput technologies have enabled extensive genome, and transcriptome sequencing unveiling, among others, the regulatory potential of microRNAs (miRNAs). Compelling evidence shown that miRNA are attractive therapeutic targets and promising candidates as biomarkers for various therapy-resistant tumors. The analysis of miRNA profile successfully identified miR-181c and -181d as significantly downregul...
Source: Clinical and Experimental Medicine - April 10, 2024 Category: Research Source Type: research
Supporting the decision to perform molecular profiling for cancer patients based on routinely collected data through the use of machine learning
ConclusionBased on a historical cohort, our predictive model has the potential to assist clinicians in deciding whether to perform molecular profiling. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - April 10, 2024 Category: Research Source Type: research
Antinuclear antibodies may predict the development of immune-related adverse events in asymptomatic patients treated with immune checkpoint inhibitors: results from a single-center cohort
This study underlines the potential role of ANA positivity as a predictive biomarker for the development of irAEs. (Source: Clinical and Experimental Medicine)
Source: Clinical and Experimental Medicine - April 10, 2024 Category: Research Source Type: research
