Dried storage of sperm at supra-zero temperatures: an alternative for flow cytometric analysis under field conditions
Cryobiology. 2024 Apr 23:104899. doi: 10.1016/j.cryobiol.2024.104899. Online ahead of print.ABSTRACTIn biotechnological processes such as chromosomal manipulation studies, semen has become a reference in the ploidy verification of the evaluated material. However, the use of fresh samples is limited to the use at field conditions because the analysis is performed under laboratory conditions. Thus, this study aimed to develop a simpler procedure for storing dry semen at 28°C to reduce cold storage costs. For this, semen samples were evaluated according to established quality semen parameters, a protocol for dry, and 3 steri...
Source: Cryobiology - April 25, 2024 Category: Biology Authors: Jenyffer Rosero Amanda Pereira Santos Silva Silvio Carlos Alves Dos Santos George Shigueki Yasui Source Type: research
Molecular simulation on the interaction between trehalose and asymmetric lipid bilayer mimicking the membrane of human red blood cells
In this study, we conducted atomistic molecular dynamic simulations on asymmetric model bilayers that mimic the membrane of human red blood cells at various trehalose and water contents. We considered three different hydration levels mimicking the full hydration to desiccation scenarios. Results indicate that the asymmetric distribution of lipids did not significantly influence the computed structural characteristics at full and low hydration. At dehydration, however, the order parameter obtained from the symmetric bilayer is significantly higher compared to those obtained from asymmetric ones. Analysis of hydrogen bonds r...
Source: Cryobiology - April 25, 2024 Category: Biology Authors: Yu Cao Cai Gao Lei Yang Pei Zhou Dongfang Sun Source Type: research
Molecular simulation on the interaction between trehalose and asymmetric lipid bilayer mimicking the membrane of human red blood cells
In this study, we conducted atomistic molecular dynamic simulations on asymmetric model bilayers that mimic the membrane of human red blood cells at various trehalose and water contents. We considered three different hydration levels mimicking the full hydration to desiccation scenarios. Results indicate that the asymmetric distribution of lipids did not significantly influence the computed structural characteristics at full and low hydration. At dehydration, however, the order parameter obtained from the symmetric bilayer is significantly higher compared to those obtained from asymmetric ones. Analysis of hydrogen bonds r...
Source: Cryobiology - April 25, 2024 Category: Biology Authors: Yu Cao Cai Gao Lei Yang Pei Zhou Dongfang Sun Source Type: research
Dried storage of sperm at supra-zero temperatures: an alternative for flow cytometric analysis under field conditions
Cryobiology. 2024 Apr 23:104899. doi: 10.1016/j.cryobiol.2024.104899. Online ahead of print.ABSTRACTIn biotechnological processes such as chromosomal manipulation studies, semen has become a reference in the ploidy verification of the evaluated material. However, the use of fresh samples is limited to the use at field conditions because the analysis is performed under laboratory conditions. Thus, this study aimed to develop a simpler procedure for storing dry semen at 28°C to reduce cold storage costs. For this, semen samples were evaluated according to established quality semen parameters, a protocol for dry, and 3 steri...
Source: Cryobiology - April 25, 2024 Category: Biology Authors: Jenyffer Rosero Amanda Pereira Santos Silva Silvio Carlos Alves Dos Santos George Shigueki Yasui Source Type: research
Comprehensive characterisation and cryopreservation optimisation of buffalo (Bubalus bubalis) adipose tissue-derived mesenchymal stem cells
Cryobiology. 2024 Apr 23;115:104896. doi: 10.1016/j.cryobiol.2024.104896. Online ahead of print.ABSTRACTOver half of the world's buffalo (Bubalus bubalis) inhabit India, and buffaloes frequently encounter health challenges that resist conventional treatments, prompting the exploration of alternative therapeutic strategies. One promising approach is stem cell therapy, particularly multipotent mesenchymal/stromal stem cells (MSCs). These cells have shown significant efficacy in addressing various diseases in livestock that exhibit resistance to conventional therapies. Adipose tissue-derived MSCs (ADSCs) have garnered attenti...
Source: Cryobiology - April 19, 2024 Category: Biology Authors: Michelle Abraham Sandeep Goel Source Type: research
Comprehensive characterisation and cryopreservation optimisation of buffalo (Bubalus bubalis) adipose tissue-derived mesenchymal stem cells
Cryobiology. 2024 Apr 17:104896. doi: 10.1016/j.cryobiol.2024.104896. Online ahead of print.ABSTRACTOver half of the world's buffalo (Bubalus bubalis) inhabit India, and buffaloes frequently encounter health challenges that resist conventional treatments, prompting the exploration of alternative therapeutic strategies. One promising approach is stem cell therapy, particularly multipotent mesenchymal/stromal stem cells (MSCs). These cells have shown significant efficacy in addressing various diseases in livestock that exhibit resistance to conventional therapies. Adipose tissue-derived MSCs (ADSCs) have garnered attention d...
Source: Cryobiology - April 19, 2024 Category: Biology Authors: Michelle Abraham Sandeep Goel Source Type: research
Lipid mixtures (from a liposome kit) and melatonin improve post-thawed Angora goat sperm parameters
This study aimed to investigate lipid mixtures (from a liposome kit, Lps) and melatonin (Mel) at different doses to prevent the deterioration of sperm parameters and to provide the cryoprotective effects on sperm DNA. The Angora goat ejaculates were collected and pooled. They were divided into seven equal volumes, and each of them was diluted with the extenders of the experimental groups with additives (Lps 321.99 μg/mL, Lps 841.33 μg/mL, Mel 0.25 mM, Mel 1 mM, Lps 321.99 μg/mL + Mel 1 mM, Lps 841.33 μg/mL + Mel 0.25 mM) and no additives (control group). After the freeze-thawing process, motility, viability, acrosome i...
Source: Cryobiology - April 18, 2024 Category: Biology Authors: Mustafa Numan Bucak Ömer Faruk Karaşör Ay şe Sarı Mustafa Bodu Pinar Ili Salih Narl ıçay Mehmet Bozkurt Ataman Fikret Sari Source Type: research
Lipid mixtures (from a liposome kit) and melatonin improve post-thawed Angora goat sperm parameters
This study aimed to investigate lipid mixtures (from a liposome kit, Lps) and melatonin (Mel) at different doses to prevent the deterioration of sperm parameters and to provide the cryoprotective effects on sperm DNA. The Angora goat ejaculates were collected and pooled. They were divided into seven equal volumes, and each of them was diluted with the extenders of the experimental groups with additives (Lps 321.99 μg/mL, Lps 841.33 μg/mL, Mel 0.25 mM, Mel 1 mM, Lps 321.99 μg/mL + Mel 1 mM, Lps 841.33 μg/mL + Mel 0.25 mM) and no additives (control group). After the freeze-thawing process, motility, viability, acrosome i...
Source: Cryobiology - April 18, 2024 Category: Biology Authors: Mustafa Numan Bucak Ömer Faruk Karaşör Ay şe Sarı Mustafa Bodu Pinar Ili Salih Narl ıçay Mehmet Bozkurt Ataman Fikret Sari Source Type: research
Effect of resuscitation of cryopreserved porcine adrenal glands at 26 °C on their recovery and functioning under xenotransplantation
Cryobiology. 2024 Apr 16;115:104895. doi: 10.1016/j.cryobiol.2024.104895. Online ahead of print.ABSTRACTThe study is devoted to the effect of lowered resuscitation temperature (26 °C) on cryopreserved porcine adrenal glands functional activity in vitro and in vivo under xenotransplantation. The adrenals were collected from newborn pigs, cryopreserved with 5 % DMSO at a rate of 1 °C/min, resuscitated at 26 or 37 °C for 48 h (5 % CO2, DMEM), embedded into small intestinal submucosa, and transplanted to bilaterally adrenalectomized rats. It has been shown that the glands resuscitated at 26 °C have suppressed free-radical ...
Source: Cryobiology - April 14, 2024 Category: Biology Authors: Shasha Li Lingling Fan Ustichenko Viktoria Pakhomov Oleksandr Zhongjie Li Wenlu Zhang Bo Deng Source Type: research
Effect of resuscitation of cryopreserved porcine adrenal glands at 26 °C on their recovery and functioning under xenotransplantation
Cryobiology. 2024 Apr 12:104895. doi: 10.1016/j.cryobiol.2024.104895. Online ahead of print.ABSTRACTThe study is devoted to the effect of lowered resuscitation temperature (26 °C) on cryopreserved porcine adrenal glands functional activity in vitro and in vivo under xenotransplantation. The adrenals were collected from newborn pigs, cryopreserved with 5% DMSO at a rate of 1 °C/min, resuscitated at 26 or 37 °C for 48 hours (5% CO2, DMEM), embedded into small intestinal submucosa, and transplanted to bilaterally adrenalectomized rats. It has been shown that the glands resuscitated at 26 °C have suppressed free-radical pr...
Source: Cryobiology - April 14, 2024 Category: Biology Authors: Shasha Li Lingling Fan Ustichenko Viktoria Pakhomov Oleksandr Zhongjie Li Wenlu Zhang Bo Deng Source Type: research
Effect of resuscitation of cryopreserved porcine adrenal glands at 26 °C on their recovery and functioning under xenotransplantation
Cryobiology. 2024 Apr 12:104895. doi: 10.1016/j.cryobiol.2024.104895. Online ahead of print.ABSTRACTThe study is devoted to the effect of lowered resuscitation temperature (26 °C) on cryopreserved porcine adrenal glands functional activity in vitro and in vivo under xenotransplantation. The adrenals were collected from newborn pigs, cryopreserved with 5% DMSO at a rate of 1 °C/min, resuscitated at 26 or 37 °C for 48 hours (5% CO2, DMEM), embedded into small intestinal submucosa, and transplanted to bilaterally adrenalectomized rats. It has been shown that the glands resuscitated at 26 °C have suppressed free-radical pr...
Source: Cryobiology - April 14, 2024 Category: Biology Authors: Shasha Li Lingling Fan Ustichenko Viktoria Pakhomov Oleksandr Zhongjie Li Wenlu Zhang Bo Deng Source Type: research
In vivo study of porous NiTi cryotweezers for bone tissue cryotherapy
This study examined the effects of liquid nitrogen vapor on osteogenesis in the rabbit femur. Cryotweezers made of porous nickel titanium alloy (nitinol or NiTi) obtained by self-propagating high temperature synthesis were used in this experiment. The porous structure of the cryotweezers allows them to hold up to 10 g of liquid nitrogen after being immersed for 2 minutes, which completely evaporates after 160 seconds. To study the effects of liquid nitrogen evaporation on osteogenesis, a rabbit femur was perforated. The formed holes were subjected to cryotherapy with varying exposure times. It was found that a 3 second exp...
Source: Cryobiology - April 13, 2024 Category: Biology Authors: Ekaterina S Marchenko Kirill M Dubovikov Ivan I Kuzhelivskiy Maksim O Pleshkov Evgeniy S Koroluk Konstantin S Brazovskii Alex A Volinsky Source Type: research
27 MHz constant field dielectric warming of kidneys cryopreserved by vitrification
Cryobiology. 2024 Apr 10:104893. doi: 10.1016/j.cryobiol.2024.104893. Online ahead of print.ABSTRACTOrgans cryopreserved by vitrification are exposed to the lowest possible concentration of cryoprotectants for the least time necessary to successfully avoid ice formation. Faster cooling and warming rates enable lower concentrations and perfusion times, reducing toxicity. Since warming rates necessary to avoid ice formation during recovery from vitrification are typically faster than cooling rates necessary for vitrification, warming speed is a major determining factor for successful vitrification. Dielectric warming uses an...
Source: Cryobiology - April 12, 2024 Category: Biology Authors: Brian Wowk John Phan Roberto Pagotan Erika Galvez Gregory M Fahy Source Type: research
The effects of repeated freezing and thawing on bovine sperm morphometry and function
Cryobiology. 2024 Apr 10;115:104892. doi: 10.1016/j.cryobiol.2024.104892. Online ahead of print.ABSTRACTRefreezing the remaining genetic resources after in vitro fertilization (IVF) can conserve genetic materials. However, the precise damage inflicted by repeated freezing and thawing on bovine sperm and its underlying mechanism remain largely unexplored. Thus, this study investigates the impact of repeated freeze-thaw cycles on sperm. Our findings indicate that such cycles significantly reduce sperm viability and motility. Furthermore, the integrity of the sperm plasma membrane and acrosome is compromised during this proce...
Source: Cryobiology - April 9, 2024 Category: Biology Authors: Chang-Guo Min Xin Ma Yu-Chan Wang Cheng-Kun Zhong Chong-Shan Yuan Kai-Yan Zhang Cheng-Lin Zhan Sheng-Kui Hou Xin-Hai Wang Jun Wang Jing Zhao Yi Fang Hong-Yu Liu He Ding Jing Guo Wen-Fa Lu Source Type: research
The effects of repeated freezing and thawing on bovine sperm morphometry and function
Cryobiology. 2024 Apr 10;115:104892. doi: 10.1016/j.cryobiol.2024.104892. Online ahead of print.ABSTRACTRefreezing the remaining genetic resources after in vitro fertilization (IVF) can conserve genetic materials. However, the precise damage inflicted by repeated freezing and thawing on bovine sperm and its underlying mechanism remain largely unexplored. Thus, this study investigates the impact of repeated freeze-thaw cycles on sperm. Our findings indicate that such cycles significantly reduce sperm viability and motility. Furthermore, the integrity of the sperm plasma membrane and acrosome is compromised during this proce...
Source: Cryobiology - April 9, 2024 Category: Biology Authors: Chang-Guo Min Xin Ma Yu-Chan Wang Cheng-Kun Zhong Chong-Shan Yuan Kai-Yan Zhang Cheng-Lin Zhan Sheng-Kui Hou Xin-Hai Wang Jun Wang Jing Zhao Yi Fang Hong-Yu Liu He Ding Jing Guo Wen-Fa Lu Source Type: research
