Developmental and Comparative Immunology 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Diversification of Toll-like receptor 1 in swamp eel (Monopterus albus)
In this study, the genetic characteristic of TLR1 was studied for an important aquaculture fish, swamp eel Monopterus albus. The eel has been seriously threatened by infectious diseases. However, a low level of genetic heterogeneity in the fish that has resulted from a demographic bottleneck presents further challenges in breeding for disease resistance. A comparison with the homologue of closely related species M. javanensis revealed that amino acid replacement (nonsynonymous) but not silent (synonymous) differences have accumulated nonrandomly over the coding sequences of the receptors at the early stage of their phyloge...
Source: Developmental and Comparative Immunology - May 2, 2024 Category: Allergy & Immunology Authors: Han Rao Haifeng Tian Xueting Wang Caifei Huo Lilan Zhu Zhong Li Yan Li Source Type: research
Analysis of tissue tropism of GCRV-II infection in grass carp using a VP35 monoclonal antibody
In this study, monoclonal antibodies against recombinant VP35 protein were generated in mice and characterized. The antibodies exhibited specific binding to the N terminal region (1-155 aa) of the recombinant VP35 protein expressed in the HEK293 cells, and native VP35 protein in the GCRV-II infected CIK cells. Immunofluorescent staining revealed that viruses aggregated in the cytoplasm of infected cells. In vivo challenge experiments showed that high levels of GCRV-II viruses were present in the gills, intestine, spleen and liver, indicating that they are the major sites for virus infection. Our study showed that the VP35 ...
Source: Developmental and Comparative Immunology - May 1, 2024 Category: Allergy & Immunology Authors: Yanan Lu Weihua Zhao Ning Ji Dan Xu Yaoguo Li Tiaoyi Xiao Junya Wang Jun Zou Source Type: research
Analysis of tissue tropism of GCRV-II infection in grass carp using a VP35 monoclonal antibody
In this study, monoclonal antibodies against recombinant VP35 protein were generated in mice and characterized. The antibodies exhibited specific binding to the N terminal region (1-155 aa) of the recombinant VP35 protein expressed in the HEK293 cells, and native VP35 protein in the GCRV-II infected CIK cells. Immunofluorescent staining revealed that viruses aggregated in the cytoplasm of infected cells. In vivo challenge experiments showed that high levels of GCRV-II viruses were present in the gills, intestine, spleen and liver, indicating that they are the major sites for virus infection. Our study showed that the VP35 ...
Source: Developmental and Comparative Immunology - May 1, 2024 Category: Allergy & Immunology Authors: Yanan Lu Weihua Zhao Ning Ji Dan Xu Yaoguo Li Tiaoyi Xiao Junya Wang Jun Zou Source Type: research
Induction of Trained Immunity using β-glucan and its protective responses in Nile tilapia, Oreochromis niloticus
Dev Comp Immunol. 2024 Apr 25:105188. doi: 10.1016/j.dci.2024.105188. Online ahead of print.ABSTRACTEmerging and re-emerging diseases in fish cause drastic economic losses in the aquaculture sector. To combat the impact of disease outbreaks and prevent the emergence of infections in culture systems, understanding the advanced strategies for protecting fish against infections is inevitable in fish health research. Therefore, the present study aimed to evaluate the induction of trained immunity and its protective efficacy against Streptococcus agalactiae in tilapia. For this, Nile tilapia and the primary head kidney macropha...
Source: Developmental and Comparative Immunology - April 27, 2024 Category: Allergy & Immunology Authors: David Waikhom Jeena Kezhedath Sooraj Nediyirippil Suresh Megha Kadam Bedekar Tincy Varghese Pani Prasad Kurcheti Rajendran Kooloth Valappil Source Type: research
Induction of Trained Immunity using β-glucan and its protective responses in Nile tilapia, Oreochromis niloticus
Dev Comp Immunol. 2024 Apr 25:105188. doi: 10.1016/j.dci.2024.105188. Online ahead of print.ABSTRACTEmerging and re-emerging diseases in fish cause drastic economic losses in the aquaculture sector. To combat the impact of disease outbreaks and prevent the emergence of infections in culture systems, understanding the advanced strategies for protecting fish against infections is inevitable in fish health research. Therefore, the present study aimed to evaluate the induction of trained immunity and its protective efficacy against Streptococcus agalactiae in tilapia. For this, Nile tilapia and the primary head kidney macropha...
Source: Developmental and Comparative Immunology - April 27, 2024 Category: Allergy & Immunology Authors: David Waikhom Jeena Kezhedath Sooraj Nediyirippil Suresh Megha Kadam Bedekar Tincy Varghese Pani Prasad Kurcheti Rajendran Kooloth Valappil Source Type: research
Induction of Trained Immunity using β-glucan and its protective responses in Nile tilapia, Oreochromis niloticus
Dev Comp Immunol. 2024 Apr 25:105188. doi: 10.1016/j.dci.2024.105188. Online ahead of print.ABSTRACTEmerging and re-emerging diseases in fish cause drastic economic losses in the aquaculture sector. To combat the impact of disease outbreaks and prevent the emergence of infections in culture systems, understanding the advanced strategies for protecting fish against infections is inevitable in fish health research. Therefore, the present study aimed to evaluate the induction of trained immunity and its protective efficacy against Streptococcus agalactiae in tilapia. For this, Nile tilapia and the primary head kidney macropha...
Source: Developmental and Comparative Immunology - April 27, 2024 Category: Allergy & Immunology Authors: David Waikhom Jeena Kezhedath Sooraj Nediyirippil Suresh Megha Kadam Bedekar Tincy Varghese Pani Prasad Kurcheti Rajendran Kooloth Valappil Source Type: research
The differential impact of iron on ferroptosis, oxidative stress, and inflammatory reaction in head-kidney macrophages of yellow catfish (Pelteobagrus fulvidraco) with and without ammonia stress
This study aimed to investigate the effect of iron on indicators of fish exposed to ammonia, specifically on the three aspects mentioned above. The head kidney macrophages of yellow catfish were randomly assigned to one of four groups: CON (normal control), AM (0.046 mg L-1 total ammonia nitrogen), Fe (20 μg mL-1 FeSO4), and Fe+AM (20 μg mL-1 FeSO4, 0.046 mg L-1 total ammonia nitrogen). The cells were pretreated with FeSO4 for 6 hours followed by ammonia for 24 hours. The study found that iron supplementation led to an excessive accumulation of iron and ROS in macrophages, but it did not strongly induce ferroptosis, oxid...
Source: Developmental and Comparative Immunology - April 21, 2024 Category: Allergy & Immunology Authors: Kewei He Xinran Long Haibo Jiang Chuanjie Qin Source Type: research
The differential impact of iron on ferroptosis, oxidative stress, and inflammatory reaction in head-kidney macrophages of yellow catfish (Pelteobagrus fulvidraco) with and without ammonia stress
This study aimed to investigate the effect of iron on indicators of fish exposed to ammonia, specifically on the three aspects mentioned above. The head kidney macrophages of yellow catfish were randomly assigned to one of four groups: CON (normal control), AM (0.046 mg L-1 total ammonia nitrogen), Fe (20 μg mL-1 FeSO4), and Fe+AM (20 μg mL-1 FeSO4, 0.046 mg L-1 total ammonia nitrogen). The cells were pretreated with FeSO4 for 6 hours followed by ammonia for 24 hours. The study found that iron supplementation led to an excessive accumulation of iron and ROS in macrophages, but it did not strongly induce ferroptosis, oxid...
Source: Developmental and Comparative Immunology - April 21, 2024 Category: Allergy & Immunology Authors: Kewei He Xinran Long Haibo Jiang Chuanjie Qin Source Type: research
The differential impact of iron on ferroptosis, oxidative stress, and inflammatory reaction in head-kidney macrophages of yellow catfish (Pelteobagrus fulvidraco) with and without ammonia stress
This study aimed to investigate the effect of iron on indicators of fish exposed to ammonia, specifically on the three aspects mentioned above. The head kidney macrophages of yellow catfish were randomly assigned to one of four groups: CON (normal control), AM (0.046 mg L-1 total ammonia nitrogen), Fe (20 μg mL-1 FeSO4), and Fe+AM (20 μg mL-1 FeSO4, 0.046 mg L-1 total ammonia nitrogen). The cells were pretreated with FeSO4 for 6 hours followed by ammonia for 24 hours. The study found that iron supplementation led to an excessive accumulation of iron and ROS in macrophages, but it did not strongly induce ferroptosis, oxid...
Source: Developmental and Comparative Immunology - April 21, 2024 Category: Allergy & Immunology Authors: Kewei He Xinran Long Haibo Jiang Chuanjie Qin Source Type: research
The differential impact of iron on ferroptosis, oxidative stress, and inflammatory reaction in head-kidney macrophages of yellow catfish (Pelteobagrus fulvidraco) with and without ammonia stress
This study aimed to investigate the effect of iron on indicators of fish exposed to ammonia, specifically on the three aspects mentioned above. The head kidney macrophages of yellow catfish were randomly assigned to one of four groups: CON (normal control), AM (0.046 mg L-1 total ammonia nitrogen), Fe (20 μg mL-1 FeSO4), and Fe+AM (20 μg mL-1 FeSO4, 0.046 mg L-1 total ammonia nitrogen). The cells were pretreated with FeSO4 for 6 hours followed by ammonia for 24 hours. The study found that iron supplementation led to an excessive accumulation of iron and ROS in macrophages, but it did not strongly induce ferroptosis, oxid...
Source: Developmental and Comparative Immunology - April 21, 2024 Category: Allergy & Immunology Authors: Kewei He Xinran Long Haibo Jiang Chuanjie Qin Source Type: research
The differential impact of iron on ferroptosis, oxidative stress, and inflammatory reaction in head-kidney macrophages of yellow catfish (Pelteobagrus fulvidraco) with and without ammonia stress
This study aimed to investigate the effect of iron on indicators of fish exposed to ammonia, specifically on the three aspects mentioned above. The head kidney macrophages of yellow catfish were randomly assigned to one of four groups: CON (normal control), AM (0.046 mg L-1 total ammonia nitrogen), Fe (20 μg mL-1 FeSO4), and Fe+AM (20 μg mL-1 FeSO4, 0.046 mg L-1 total ammonia nitrogen). The cells were pretreated with FeSO4 for 6 hours followed by ammonia for 24 hours. The study found that iron supplementation led to an excessive accumulation of iron and ROS in macrophages, but it did not strongly induce ferroptosis, oxid...
Source: Developmental and Comparative Immunology - April 21, 2024 Category: Allergy & Immunology Authors: Kewei He Xinran Long Haibo Jiang Chuanjie Qin Source Type: research
Effect of F-isoprostane class on cow peripheral blood neutrophil microbicidal function in vitro
Dev Comp Immunol. 2024 Apr 17;156:105180. doi: 10.1016/j.dci.2024.105180. Online ahead of print.ABSTRACTIsoprostanes (isoP) are formed during conditions of oxidative stress (OS) through the oxidation of cell membrane fatty acids. Different classes of isoP are formed depending on the fatty acid being oxidized but the biological activity of these molecules in innate immune cells is poorly understood. Thus, the objective of this study was to compare in vitro the effects of F2- and F3-isoP on neutrophil microbicidal functions. We isolated neutrophils from 6 dairy cows and incubated them for 8 h at various concentrations of F2-...
Source: Developmental and Comparative Immunology - April 19, 2024 Category: Allergy & Immunology Authors: Eric J Owczarzak Angel Abuelo Source Type: research
Fish ubiquitin-specific protease 8 (USP8) inhibits IFN production through autophagy-lysosomal dependent degradation of IRF7
In this study, we report that zebrafish ubiquitin-specific protease 8 (USP8) promotes IRF7 degradation through an autophagy-lysosome-dependent pathway to inhibit IFN production. First, zebrafish usp8 is induced upon spring viremia of carp virus (SVCV) infection and polyinosinic/polycytidylic acid (poly I:C) stimulation. Second, overexpression of USP8 suppresses SVCV or poly I:C-mediated IFN expression. Mechanistically, USP8 interacts with IRF7 and promotes its degradation via an autophagy-lysosome-dependent pathway. Finally, USP8 significantly suppresses cellular antiviral responses and enhances SVCV proliferation. In summ...
Source: Developmental and Comparative Immunology - April 18, 2024 Category: Allergy & Immunology Authors: Chu-Jing Zhou Can Zhang Long-Feng Lu Shun Li Source Type: research
Study on anti-BmNPV mechanism of branched-chain amino acid aminotransferases in silkworm
In this study, the four midgut tissue samples of the resistance strain NB (NB) and susceptible strain 306 (306) and the NB and 306 continuously infected with BmNPV for 96 h are used for whole transcriptome sequencing to analyze the differences in the genetic background of NB and 306 and the differences after inoculation of BmNPV, and the significantly different mRNA, miRNA and lnRNA between NB and 306 after BmNPV inoculation were screened. By comparing NB and 306, 2651 significantly different mRNAs, 57 significantly different miRNAs and 198 significantly different lncRNAs were screened. By comparing NB and 306 after BmNPV ...
Source: Developmental and Comparative Immunology - April 18, 2024 Category: Allergy & Immunology Authors: Can Chen Liang Chen Xiaoyong Liu Shangshang Ma Keping Chen Source Type: research
Galectin-8-like isoform X1 mediates antibacterial, antiviral, and antioxidant responses in red-lip mullet (Planiliza haematocheilus) through positive modulation of pro-inflammatory cytokine, chemokine, and enzymatic antioxidant activity
This study identified Galectin-8-like isoform X1 (PhGal8X1) from red-lip mullet (Planiliza haematocheilus) and investigated its role in regulating fish immunity. The open reading frame of PhGal8X1 was 918bp, encoding a soluble protein of 305 amino acids. The protein had a theoretical isoelectric (pI) point of 7.7 and an estimated molecular weight of 34.078 kDa. PhGal8X1 was expressed in various tissues of the fish, with prominent levels in the brain, stomach, and intestine. PhGal8X1 expression was significantly (p<0.05) induced in the blood and spleen upon challenge with different immune stimuli, including polyinosinic:...
Source: Developmental and Comparative Immunology - April 18, 2024 Category: Allergy & Immunology Authors: W A D L R Warnakula H M V Udayantha D S Liyanage E M T Tharanga W K M Omeka M A H Dilshan H A C R Hanchapola J D H E Jayasinghe Taehyug Jeong Qiang Wan Jehee Lee Source Type: research
