Notochord segmentation in zebrafish controlled by iterative mechanical signaling
Dev Cell. 2024 Apr 26:S1534-5807(24)00238-7. doi: 10.1016/j.devcel.2024.04.013. Online ahead of print.ABSTRACTIn bony fishes, patterning of the vertebral column, or spine, is guided by a metameric blueprint established in the notochord sheath. Notochord segmentation begins days after somitogenesis concludes and can occur in its absence. However, somite patterning defects lead to imprecise notochord segmentation, suggesting that these processes are linked. Here, we identify that interactions between the notochord and the axial musculature ensure precise spatiotemporal segmentation of the zebrafish spine. We demonstrate that...
Source: Developmental Cell - May 2, 2024 Category: Cytology Authors: Susan Wopat Priyom Adhyapok Bijoy Daga Janice M Crawford James Norman Jennifer Bagwell Brianna Peskin Indrasen Magre Stephanie M Fogerson Daniel S Levic Stefano Di Talia Daniel P Kiehart Patrick Charbonneau Michel Bagnat Source Type: research
A cellular tilting mechanism important for dynamic tissue shape changes and cell differentiation in Drosophila
Dev Cell. 2024 Apr 29:S1534-5807(24)00236-3. doi: 10.1016/j.devcel.2024.04.011. Online ahead of print.ABSTRACTDynamic changes in three-dimensional cell shape are important for tissue form and function. In the developing Drosophila eye, photoreceptor differentiation requires the progression across the tissue of an epithelial fold known as the morphogenetic furrow. Morphogenetic furrow progression involves apical cell constriction and movement of apical cell edges. Here, we show that cells progressing through the morphogenetic furrow move their basal edges in opposite direction to their apical edges, resulting in a cellular ...
Source: Developmental Cell - May 1, 2024 Category: Cytology Authors: Liyuan Sui Christian Dahmann Source Type: research
A cellular tilting mechanism important for dynamic tissue shape changes and cell differentiation in Drosophila
Dev Cell. 2024 Apr 29:S1534-5807(24)00236-3. doi: 10.1016/j.devcel.2024.04.011. Online ahead of print.ABSTRACTDynamic changes in three-dimensional cell shape are important for tissue form and function. In the developing Drosophila eye, photoreceptor differentiation requires the progression across the tissue of an epithelial fold known as the morphogenetic furrow. Morphogenetic furrow progression involves apical cell constriction and movement of apical cell edges. Here, we show that cells progressing through the morphogenetic furrow move their basal edges in opposite direction to their apical edges, resulting in a cellular ...
Source: Developmental Cell - May 1, 2024 Category: Cytology Authors: Liyuan Sui Christian Dahmann Source Type: research
A critical suppression feedback loop determines soybean photoperiod sensitivity
Dev Cell. 2024 Apr 23:S1534-5807(24)00229-6. doi: 10.1016/j.devcel.2024.04.004. Online ahead of print.ABSTRACTPhotoperiod sensitivity is crucial for soybean flowering, adaptation, and yield. In soybean, photoperiod sensitivity centers around the evening complex (EC) that regulates the transcriptional level of the core transcription factor E1, thereby regulating flowering. However, little is known about the regulation of the activity of EC. Our study identifies how E2/GIGANTEA (GI) and its homologs modulate photoperiod sensitivity through interactions with the EC. During long days, E2 interacts with the blue-light receptor ...
Source: Developmental Cell - April 30, 2024 Category: Cytology Authors: Xiaohui Zhao Haiyang Li Lingshuang Wang Jianhao Wang Zerong Huang Haiping Du Yaru Li Jiahui Yang Milan He Qun Cheng Xiaoya Lin Baohui Liu Fanjiang Kong Source Type: research
Photoregulatory protein kinases fine-tune plant photomorphogenesis by directing a bifunctional phospho-code on HY5 in Arabidopsis
Dev Cell. 2024 Apr 23:S1534-5807(24)00232-6. doi: 10.1016/j.devcel.2024.04.007. Online ahead of print.ABSTRACTPhotomorphogenesis is a light-dependent plant growth and development program. As the core regulator of photomorphogenesis, ELONGATED HYPOCOTYL 5 (HY5) is affected by dynamic changes in its transcriptional activity and protein stability; however, little is known about the mediators of these processes. Here, we identified PHOTOREGULATORY PROTEIN KINASE 1 (PPK1), which interacts with and phosphorylates HY5 in Arabidopsis, as one such mediator. The phosphorylation of HY5 by PPK1 is essential to establish high-affinity ...
Source: Developmental Cell - April 27, 2024 Category: Cytology Authors: Nan Zhang Chuang-Qi Wei Da-Jin Xu Zhi-Ping Deng Ya-Chao Zhao Lian-Feng Ai Ying Sun Zhi-Yong Wang Sheng-Wei Zhang Source Type: research
Photoregulatory protein kinases fine-tune plant photomorphogenesis by directing a bifunctional phospho-code on HY5 in Arabidopsis
Dev Cell. 2024 Apr 23:S1534-5807(24)00232-6. doi: 10.1016/j.devcel.2024.04.007. Online ahead of print.ABSTRACTPhotomorphogenesis is a light-dependent plant growth and development program. As the core regulator of photomorphogenesis, ELONGATED HYPOCOTYL 5 (HY5) is affected by dynamic changes in its transcriptional activity and protein stability; however, little is known about the mediators of these processes. Here, we identified PHOTOREGULATORY PROTEIN KINASE 1 (PPK1), which interacts with and phosphorylates HY5 in Arabidopsis, as one such mediator. The phosphorylation of HY5 by PPK1 is essential to establish high-affinity ...
Source: Developmental Cell - April 27, 2024 Category: Cytology Authors: Nan Zhang Chuang-Qi Wei Da-Jin Xu Zhi-Ping Deng Ya-Chao Zhao Lian-Feng Ai Ying Sun Zhi-Yong Wang Sheng-Wei Zhang Source Type: research
Photoregulatory protein kinases fine-tune plant photomorphogenesis by directing a bifunctional phospho-code on HY5 in Arabidopsis
Dev Cell. 2024 Apr 23:S1534-5807(24)00232-6. doi: 10.1016/j.devcel.2024.04.007. Online ahead of print.ABSTRACTPhotomorphogenesis is a light-dependent plant growth and development program. As the core regulator of photomorphogenesis, ELONGATED HYPOCOTYL 5 (HY5) is affected by dynamic changes in its transcriptional activity and protein stability; however, little is known about the mediators of these processes. Here, we identified PHOTOREGULATORY PROTEIN KINASE 1 (PPK1), which interacts with and phosphorylates HY5 in Arabidopsis, as one such mediator. The phosphorylation of HY5 by PPK1 is essential to establish high-affinity ...
Source: Developmental Cell - April 27, 2024 Category: Cytology Authors: Nan Zhang Chuang-Qi Wei Da-Jin Xu Zhi-Ping Deng Ya-Chao Zhao Lian-Feng Ai Ying Sun Zhi-Yong Wang Sheng-Wei Zhang Source Type: research
The Zn < sup > 2+ < /sup > transporter ZIP7 enhances endoplasmic-reticulum-associated protein degradation and prevents neurodegeneration in Drosophila
Dev Cell. 2024 Apr 22:S1534-5807(24)00228-4. doi: 10.1016/j.devcel.2024.04.003. Online ahead of print.ABSTRACTProteotoxic stress drives numerous degenerative diseases. Cells initially adapt to misfolded proteins by activating the unfolded protein response (UPR), including endoplasmic-reticulum-associated protein degradation (ERAD). However, persistent stress triggers apoptosis. Enhancing ERAD is a promising therapeutic approach for protein misfolding diseases. The ER-localized Zn2+ transporter ZIP7 is conserved from plants to humans and required for intestinal self-renewal, Notch signaling, cell motility, and survival. How...
Source: Developmental Cell - April 26, 2024 Category: Cytology Authors: Xiaoran Guo Morgan Mutch Alba Yurani Torres Maddalena Nano Nishi Rauth Jacob Harwood Drew McDonald Zijing Chen Craig Montell Wei Dai Denise J Montell Source Type: research
Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans
Dev Cell. 2024 Apr 24:S1534-5807(24)00230-2. doi: 10.1016/j.devcel.2024.04.005. Online ahead of print.ABSTRACTFor an organ to maintain correct architecture and function, its diverse cellular components must coordinate their size and shape. Although cell-intrinsic mechanisms driving homotypic cell-cell coordination are known, it is unclear how cell shape is regulated across heterotypic cells. We find that epithelial cells maintain the shape of neighboring sense-organ glia-neuron units in adult Caenorhabditis elegans (C. elegans). Hsp co-chaperone UNC-23/BAG2 prevents epithelial cell shape from deforming, and its loss causes...
Source: Developmental Cell - April 26, 2024 Category: Cytology Authors: Cecilia G Martin James S Bent Tyler Hill Irini Topalidou Aakanksha Singhvi Source Type: research
The Drosophila Hippo pathway transcription factor Scalloped and its co-factors alter each other's chromatin binding dynamics and transcription in vivo
Dev Cell. 2024 Apr 23:S1534-5807(24)00231-4. doi: 10.1016/j.devcel.2024.04.006. Online ahead of print.ABSTRACTThe Hippo pathway is an important regulator of organ growth and cell fate. The major mechanism by which Hippo is known to control transcription is by dictating the nucleo-cytoplasmic shuttling rate of Yorkie, a transcription co-activator, which promotes transcription with the DNA binding protein Scalloped. The nuclear biophysical behavior of Yorkie and Scalloped, and whether this is regulated by the Hippo pathway, remains unexplored. Using multiple live-imaging modalities on Drosophila tissues, we found that Scallo...
Source: Developmental Cell - April 26, 2024 Category: Cytology Authors: Samuel A Manning Benjamin Kroeger Qiji Deng Elliot Brooks Yoshana Fonseka Elizabeth Hinde Kieran F Harvey Source Type: research
The Zn < sup > 2+ < /sup > transporter ZIP7 enhances endoplasmic-reticulum-associated protein degradation and prevents neurodegeneration in Drosophila
Dev Cell. 2024 Apr 22:S1534-5807(24)00228-4. doi: 10.1016/j.devcel.2024.04.003. Online ahead of print.ABSTRACTProteotoxic stress drives numerous degenerative diseases. Cells initially adapt to misfolded proteins by activating the unfolded protein response (UPR), including endoplasmic-reticulum-associated protein degradation (ERAD). However, persistent stress triggers apoptosis. Enhancing ERAD is a promising therapeutic approach for protein misfolding diseases. The ER-localized Zn2+ transporter ZIP7 is conserved from plants to humans and required for intestinal self-renewal, Notch signaling, cell motility, and survival. How...
Source: Developmental Cell - April 26, 2024 Category: Cytology Authors: Xiaoran Guo Morgan Mutch Alba Yurani Torres Maddalena Nano Nishi Rauth Jacob Harwood Drew McDonald Zijing Chen Craig Montell Wei Dai Denise J Montell Source Type: research
Epithelial UNC-23 limits mechanical stress to maintain glia-neuron architecture in C. elegans
Dev Cell. 2024 Apr 24:S1534-5807(24)00230-2. doi: 10.1016/j.devcel.2024.04.005. Online ahead of print.ABSTRACTFor an organ to maintain correct architecture and function, its diverse cellular components must coordinate their size and shape. Although cell-intrinsic mechanisms driving homotypic cell-cell coordination are known, it is unclear how cell shape is regulated across heterotypic cells. We find that epithelial cells maintain the shape of neighboring sense-organ glia-neuron units in adult Caenorhabditis elegans (C. elegans). Hsp co-chaperone UNC-23/BAG2 prevents epithelial cell shape from deforming, and its loss causes...
Source: Developmental Cell - April 26, 2024 Category: Cytology Authors: Cecilia G Martin James S Bent Tyler Hill Irini Topalidou Aakanksha Singhvi Source Type: research
The Drosophila Hippo pathway transcription factor Scalloped and its co-factors alter each other's chromatin binding dynamics and transcription in vivo
Dev Cell. 2024 Apr 23:S1534-5807(24)00231-4. doi: 10.1016/j.devcel.2024.04.006. Online ahead of print.ABSTRACTThe Hippo pathway is an important regulator of organ growth and cell fate. The major mechanism by which Hippo is known to control transcription is by dictating the nucleo-cytoplasmic shuttling rate of Yorkie, a transcription co-activator, which promotes transcription with the DNA binding protein Scalloped. The nuclear biophysical behavior of Yorkie and Scalloped, and whether this is regulated by the Hippo pathway, remains unexplored. Using multiple live-imaging modalities on Drosophila tissues, we found that Scallo...
Source: Developmental Cell - April 26, 2024 Category: Cytology Authors: Samuel A Manning Benjamin Kroeger Qiji Deng Elliot Brooks Yoshana Fonseka Elizabeth Hinde Kieran F Harvey Source Type: research
Cryo-EM structures of membrane-bound dynamin in a post-hydrolysis state primed for membrane fission
Dev Cell. 2024 Apr 23:S1534-5807(24)00233-8. doi: 10.1016/j.devcel.2024.04.008. Online ahead of print.ABSTRACTDynamin assembles as a helical polymer at the neck of budding endocytic vesicles, constricting the underlying membrane as it progresses through the GTPase cycle to sever vesicles from the plasma membrane. Although atomic models of the dynamin helical polymer bound to guanosine triphosphate (GTP) analogs define earlier stages of membrane constriction, there are no atomic models of the assembled state post-GTP hydrolysis. Here, we used cryo-EM methods to determine atomic structures of the dynamin helical polymer asse...
Source: Developmental Cell - April 25, 2024 Category: Cytology Authors: John R Jimah Nidhi Kundu Abigail E Stanton Kem A Sochacki Bertram Canagarajah Lieza Chan Marie-Paule Strub Huaibin Wang Justin W Taraska Jenny E Hinshaw Source Type: research
Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation
Dev Cell. 2024 Apr 21:S1534-5807(24)00234-X. doi: 10.1016/j.devcel.2024.04.009. Online ahead of print.ABSTRACTPlacental ischemia, resulting from inadequate remodeling of uterine spiral arteries, is a factor in the development of preeclampsia. However, the effect of endothelial progenitor cells that play a role in the vascular injury-repair program is largely unexplored during remodeling. Here, we observe that preeclampsia-afflicted uterine spiral arteries transition to a synthetic phenotype in vascular smooth muscle cells and characterize the regulatory axis in endothelial progenitor cells during remodeling in human decidu...
Source: Developmental Cell - April 25, 2024 Category: Cytology Authors: Bin Tan Li Lin Yu Yuan Yao Long Yi Kang Biao Huang Li-Fei Huang Jian-Hua Li Chao Tong Hong-Bo Qi Source Type: research
