Raman Spectroscopy Analysis of Human Amniotic Fluid Cells from fetuses with Myelomeningocele
Exp Cell Res. 2024 Apr 30:114048. doi: 10.1016/j.yexcr.2024.114048. Online ahead of print.ABSTRACTPrenatal surgery for the treatment of spina bifida (myelomeningocele, MMC) significantly enhances the neurological prognosis of the patient. To ensure better protection of the spinal cord by large defects, the application of skin grafts produced with cells gained from the amniotic fluid is presently studied. In order to determine the most appropriate cells for this purpose, we tried to shed light on the extremely complex amniotic fluid cellular composition in healthy and MMC pregnancies. We exploited the potential of micro-Ram...
Source: Experimental Cell Research - May 2, 2024 Category: Cytology Authors: Luca Pontiggia Katarzyna Michalak-Micka Nadine H ürlimann Hesham K Yosef Roland B öni Agnes S Klar Martin Ehrbar Nicole Ochsenbein-K ölble Thomas Biedermann Ueli Moehrlen Source Type: research
Raman Spectroscopy Analysis of Human Amniotic Fluid Cells from fetuses with Myelomeningocele
Exp Cell Res. 2024 Apr 30:114048. doi: 10.1016/j.yexcr.2024.114048. Online ahead of print.ABSTRACTPrenatal surgery for the treatment of spina bifida (myelomeningocele, MMC) significantly enhances the neurological prognosis of the patient. To ensure better protection of the spinal cord by large defects, the application of skin grafts produced with cells gained from the amniotic fluid is presently studied. In order to determine the most appropriate cells for this purpose, we tried to shed light on the extremely complex amniotic fluid cellular composition in healthy and MMC pregnancies. We exploited the potential of micro-Ram...
Source: Experimental Cell Research - May 2, 2024 Category: Cytology Authors: Luca Pontiggia Katarzyna Michalak-Micka Nadine H ürlimann Hesham K Yosef Roland B öni Agnes S Klar Martin Ehrbar Nicole Ochsenbein-K ölble Thomas Biedermann Ueli Moehrlen Source Type: research
Pyroptosis inhibitors MCC950 and VX-765 mitigate myocardial injury by alleviating oxidative stress, inflammation, and apoptosis in acute myocardial hypoxia
Exp Cell Res. 2024 Apr 29:114061. doi: 10.1016/j.yexcr.2024.114061. Online ahead of print.ABSTRACTAcute myocardial infarction (AMI) is a prevalent cardiovascular disease with high morbidity and mortality rates worldwide. Pyroptosis is an inflammatory form of programmed cell death that has been linked to various pathological conditions. However, its exact contribution to the onset and progression of heart injury in AMI has not yet fully elucidated. Herein, we established mouse AMI model by ligating the left anterior descending artery and performed transcriptome analysis during the early phase of AMI. Mouse HL-1 and human AC...
Source: Experimental Cell Research - May 1, 2024 Category: Cytology Authors: Xing Ye Zi-Jie Lin Guang-Hui Hong Zhi-Min Wang Run-Ting Dou Jun-Yi Lin Jian-Hui Xie Yi-Wen Shen Source Type: research
Pyroptosis inhibitors MCC950 and VX-765 mitigate myocardial injury by alleviating oxidative stress, inflammation, and apoptosis in acute myocardial hypoxia
Exp Cell Res. 2024 Apr 29:114061. doi: 10.1016/j.yexcr.2024.114061. Online ahead of print.ABSTRACTAcute myocardial infarction (AMI) is a prevalent cardiovascular disease with high morbidity and mortality rates worldwide. Pyroptosis is an inflammatory form of programmed cell death that has been linked to various pathological conditions. However, its exact contribution to the onset and progression of heart injury in AMI has not yet fully elucidated. Herein, we established mouse AMI model by ligating the left anterior descending artery and performed transcriptome analysis during the early phase of AMI. Mouse HL-1 and human AC...
Source: Experimental Cell Research - May 1, 2024 Category: Cytology Authors: Xing Ye Zi-Jie Lin Guang-Hui Hong Zhi-Min Wang Run-Ting Dou Jun-Yi Lin Jian-Hui Xie Yi-Wen Shen Source Type: research
Circ_PABPC1 promotes the malignancy of gastric cancer through interacting with ILK to activate NF- κB pathway
CONCLUSION: circ_PABPC1 promotes the malignancy of GC cells through binding to ILK to activate NF-κB signaling pathway .PMID:38688434 | DOI:10.1016/j.yexcr.2024.114058 (Source: Experimental Cell Research)
Source: Experimental Cell Research - April 30, 2024 Category: Cytology Authors: Siqi Yan Zhu Wang Dongqiang Lan Junjie Niu Xiaolan Jian Fengjiao He Weizhi Tang Chunhong Hu Wei Liu Source Type: research
BRAFV600E Promotes Anchorage-Independent Growth but Inhibits Anchorage-Dependent Growth in hTERT / Cdk4-Immortalized Normal Human Bronchial Epithelial Cells
In this study, we investigated whether mutant BRAF, a well-known inducer of OIS, could trigger OIS in HBEC3 cells. We also assessed the impact of mutant BRAF on the growth of HBEC3 cells, as no previous studies have examined this using a normal bronchial epithelial cell line model. We established an HBEC3 cell line, designated as HBEC3-BIN, that expresses mutant BRAFV600E in a doxycycline-regulated manner. Unlike our previous finding that KRASV12 upregulated both pERK and pAKT, mutant BRAFV600E upregulated pERK but not pAKT in HBEC3-BIN cells. Similar to KRASV12, BRAFV600E did not efficiently induce OIS. Interestingly, whi...
Source: Experimental Cell Research - April 28, 2024 Category: Cytology Authors: Nao Muraki Nozomi Kawabe Ayano Ohashi Kanna Umeda Masahito Katsuda Aya Tomatsu Mikina Yoshida Kazuki Komeda John D Minna Ichidai Tanaka Masahiro Morise Miyoko Matsushima Yusuke Matsui Tsutomu Kawabe Mitsuo Sato Source Type: research
BRAFV600E Promotes Anchorage-Independent Growth but Inhibits Anchorage-Dependent Growth in hTERT / Cdk4-Immortalized Normal Human Bronchial Epithelial Cells
In this study, we investigated whether mutant BRAF, a well-known inducer of OIS, could trigger OIS in HBEC3 cells. We also assessed the impact of mutant BRAF on the growth of HBEC3 cells, as no previous studies have examined this using a normal bronchial epithelial cell line model. We established an HBEC3 cell line, designated as HBEC3-BIN, that expresses mutant BRAFV600E in a doxycycline-regulated manner. Unlike our previous finding that KRASV12 upregulated both pERK and pAKT, mutant BRAFV600E upregulated pERK but not pAKT in HBEC3-BIN cells. Similar to KRASV12, BRAFV600E did not efficiently induce OIS. Interestingly, whi...
Source: Experimental Cell Research - April 28, 2024 Category: Cytology Authors: Nao Muraki Nozomi Kawabe Ayano Ohashi Kanna Umeda Masahito Katsuda Aya Tomatsu Mikina Yoshida Kazuki Komeda John D Minna Ichidai Tanaka Masahiro Morise Miyoko Matsushima Yusuke Matsui Tsutomu Kawabe Mitsuo Sato Source Type: research
BRAFV600E Promotes Anchorage-Independent Growth but Inhibits Anchorage-Dependent Growth in hTERT / Cdk4-Immortalized Normal Human Bronchial Epithelial Cells
In this study, we investigated whether mutant BRAF, a well-known inducer of OIS, could trigger OIS in HBEC3 cells. We also assessed the impact of mutant BRAF on the growth of HBEC3 cells, as no previous studies have examined this using a normal bronchial epithelial cell line model. We established an HBEC3 cell line, designated as HBEC3-BIN, that expresses mutant BRAFV600E in a doxycycline-regulated manner. Unlike our previous finding that KRASV12 upregulated both pERK and pAKT, mutant BRAFV600E upregulated pERK but not pAKT in HBEC3-BIN cells. Similar to KRASV12, BRAFV600E did not efficiently induce OIS. Interestingly, whi...
Source: Experimental Cell Research - April 28, 2024 Category: Cytology Authors: Nao Muraki Nozomi Kawabe Ayano Ohashi Kanna Umeda Masahito Katsuda Aya Tomatsu Mikina Yoshida Kazuki Komeda John D Minna Ichidai Tanaka Masahiro Morise Miyoko Matsushima Yusuke Matsui Tsutomu Kawabe Mitsuo Sato Source Type: research
KN-93 promotes HDAC4 nucleus translocation to promote fatty acid oxidation in myocardial infarction
In conclusion, KN-93 promotes HDAC4 translocation to the nucleus, thereby potentially enhancing fatty acid oxidation by SP1.PMID:38663474 | DOI:10.1016/j.yexcr.2024.114050 (Source: Experimental Cell Research)
Source: Experimental Cell Research - April 25, 2024 Category: Cytology Authors: Jianqiao Zhao Luona Li Xindong Wang Jianping Shen Source Type: research
PLAU promotes cell proliferation and migration of head and neck cancer via STAT3 signaling pathway
Exp Cell Res. 2024 Apr 23:114056. doi: 10.1016/j.yexcr.2024.114056. Online ahead of print.ABSTRACTIt was reported that within the head and neck cancer (HNC) cell line CAL21 the epithelial-mesenchymal transition (EMT) and cell proliferation were promoted by Urokinase-Type Plasminogen Activator (PLAU) proteinase through TNFRSF12A. Additionally, in this paper HNC cell lines refer to Fadu and Tu686. A novel PLAU-STAT3 axis was found to be involved in HNC cell line proliferation and metastasis. PLAU expression in HNC samples was upregulated, besides, the elevated expression of PLAU was linked to the lower overall survival (OS) ...
Source: Experimental Cell Research - April 25, 2024 Category: Cytology Authors: Xiaobo Cui Hongyang Sun Xiaoqing Liu Yunfei Bai Yanping Bai Yanru Cui Boqian Wang Shu Zhang Xin Li Source Type: research
Brusatol hinders the progression of bladder cancer by Chac1/Nrf2/SLC7A11 pathway
Exp Cell Res. 2024 Apr 24;438(2):114053. doi: 10.1016/j.yexcr.2024.114053. Online ahead of print.ABSTRACTBladder cancer is a common tumor that impacts the urinary system and marked by a significant fatality rate and an unfavorable prognosis. Promising antineoplastic properties are exhibited by brusatol, which is obtained from the dried ripe fruit of Brucea javanica. The present study aimed to evaluate the influence of brusatol on the progression of bladder cancer and uncover the molecular mechanism involved. We used Cell Counting Kit-8, colony formation and EdU assays to detect cell numbers, viability and proliferation. We...
Source: Experimental Cell Research - April 25, 2024 Category: Cytology Authors: X I Yu Ziqi He Qinghua Wang Shuai Ke Huaxin Wang Zhong Wang Shenglan Li Source Type: research
KN-93 Promotes HDAC4 Nucleus Translocation to promote fatty acid oxidation in Myocardial Infarction
In conclusion, KN-93 promotes HDAC4 translocation to the nucleus, thereby potentially enhancing fatty acid oxidation by SP1.PMID:38663474 | DOI:10.1016/j.yexcr.2024.114050 (Source: Experimental Cell Research)
Source: Experimental Cell Research - April 25, 2024 Category: Cytology Authors: Jianqiao Zhao Luona Li Xindong Wang Jianping Shen Source Type: research
PLAU promotes cell proliferation and migration of head and neck cancer via STAT3 signaling pathway
Exp Cell Res. 2024 Apr 23:114056. doi: 10.1016/j.yexcr.2024.114056. Online ahead of print.ABSTRACTIt was reported that within the head and neck cancer (HNC) cell line CAL21 the epithelial-mesenchymal transition (EMT) and cell proliferation were promoted by Urokinase-Type Plasminogen Activator (PLAU) proteinase through TNFRSF12A. Additionally, in this paper HNC cell lines refer to Fadu and Tu686. A novel PLAU-STAT3 axis was found to be involved in HNC cell line proliferation and metastasis. PLAU expression in HNC samples was upregulated, besides, the elevated expression of PLAU was linked to the lower overall survival (OS) ...
Source: Experimental Cell Research - April 25, 2024 Category: Cytology Authors: Xiaobo Cui Hongyang Sun Xiaoqing Liu Yunfei Bai Yanping Bai Yanru Cui Boqian Wang Shu Zhang Xin Li Source Type: research
Brusatol hinders the progression of bladder cancer by Chac1/Nrf2/SLC7A11 pathway
Exp Cell Res. 2024 Apr 23:114053. doi: 10.1016/j.yexcr.2024.114053. Online ahead of print.ABSTRACTBladder cancer is a common tumor that impacts the urinary system and marked by a significant fatality rate and an unfavorable prognosis. Promising antineoplastic properties are exhibited by brusatol, which is obtained from the dried ripe fruit of Brucea javanica. The present study aimed to evaluate the influence of brusatol on the progression of bladder cancer and uncover the molecular mechanism involved. We used Cell Counting Kit-8, colony formation and EdU assays to detect cell numbers, viability and proliferation. We used t...
Source: Experimental Cell Research - April 25, 2024 Category: Cytology Authors: X I Yu Z I Q I He Q I N G H U A Wang S H U A I Ke H U A X I N Wang Z H O N G Wang S H E N G L A N Li Source Type: research
KN-93 Promotes HDAC4 Nucleus Translocation to promote fatty acid oxidation in Myocardial Infarction
In conclusion, KN-93 promotes HDAC4 translocation to the nucleus, thereby potentially enhancing fatty acid oxidation by SP1.PMID:38663474 | DOI:10.1016/j.yexcr.2024.114050 (Source: Experimental Cell Research)
Source: Experimental Cell Research - April 25, 2024 Category: Cytology Authors: Jianqiao Zhao Luona Li Xindong Wang Jianping Shen Source Type: research
