Cyclic strain alters the transcriptional and migratory response of scleral fibroblasts to TGF β
Exp Eye Res. 2024 Apr 30:109917. doi: 10.1016/j.exer.2024.109917. Online ahead of print.ABSTRACTIn glaucoma, scleral fibroblasts are exposed to IOP-associated mechanical strain and elevated TGFβ levels. These stimuli, in turn, lead to scleral remodeling. Here, we examine the scleral fibroblast migratory and transcriptional response to these stimuli to better understand mechanisms of glaucomatous scleral remodeling. Human peripapillary scleral (PPS) fibroblasts were cultured on parallel grooves, treated with TGFβ (2 ng/ml) in the presence of vehicle or TGFβ signaling inhibitors, and exposed to uniaxial strain (1 Hz, 5%, ...
Source: Experimental Eye Research - May 2, 2024 Category: Opthalmology Authors: Ann Mozzer Ian Pitha Source Type: research
Effect of isoflurane anaesthetic time on ocular a-scan ultrasonography measures and their relationship to age and OCT measures in the guinea pig
This study shows that the depth of ocular components can change dramatically with anaesthesia. Researchers should therefore be wary of these concomitant effects and should employ adjustments to better render 'true' values.PMID:38685338 | DOI:10.1016/j.exer.2024.109914 (Source: Experimental Eye Research)
Source: Experimental Eye Research - April 30, 2024 Category: Opthalmology Authors: William E Myles Yusupjan Abdulla Sally Anne McFadden Source Type: research
Effect of isoflurane anaesthetic time on ocular a-scan ultrasonography measures and their relationship to age and OCT measures in the guinea pig
This study shows that the depth of ocular components can change dramatically with anaesthesia. Researchers should therefore be wary of these concomitant effects and should employ adjustments to better render 'true' values.PMID:38685338 | DOI:10.1016/j.exer.2024.109914 (Source: Experimental Eye Research)
Source: Experimental Eye Research - April 30, 2024 Category: Opthalmology Authors: William E Myles Yusupjan Abdulla Sally Anne McFadden Source Type: research
Effect of isoflurane anaesthetic time on ocular a-scan ultrasonography measures and their relationship to age and OCT measures in the guinea pig
This study shows that the depth of ocular components can change dramatically with anaesthesia. Researchers should therefore be wary of these concomitant effects and should employ adjustments to better render 'true' values.PMID:38685338 | DOI:10.1016/j.exer.2024.109914 (Source: Experimental Eye Research)
Source: Experimental Eye Research - April 30, 2024 Category: Opthalmology Authors: William E Myles Yusupjan Abdulla Sally Anne McFadden Source Type: research
Experimental interventions attenuate a conjunctival epidermal metaplasia model
Exp Eye Res. 2024 Apr 26:109916. doi: 10.1016/j.exer.2024.109916. Online ahead of print.ABSTRACTThe conjunctiva is a non-keratinized, stratified columnar epithelium with characteristics different from the cornea and eyelid epidermis. From development to adulthood, a distinguishing feature of ocular versus epidermal epithelia is the expression of the master regulator PAX6. A conditionally immortalized conjunctival epithelial cell line (iHCjEC) devoid of stromal or immune cells established in our laboratory spontaneously manifested epidermal metaplasia and upregulated expression of the keratinization-related genes SPRR1A/B a...
Source: Experimental Eye Research - April 28, 2024 Category: Opthalmology Authors: Takezawa Yuki Masayoshi Kamon Keiko Hiraki-Kamon Arisa Mitani Atsushi Shiraishi Hidemasa Kato Source Type: research
Raman spectroscopy assisted tear analysis: A label free, optical approach for noninvasive disease diagnostics
Exp Eye Res. 2024 Apr 26:109913. doi: 10.1016/j.exer.2024.109913. Online ahead of print.ABSTRACTIn recent times, tear fluid analysis has garnered considerable attention in the field of biomarker- based diagnostics due to its noninvasive sample collection method. Tears encompass a reservoir of biomarkers that assist in diagnosing not only ocular disorders but also a diverse list of systemic diseases. This highlights the necessity for sensitive and dependable screening methods to employ tear fluid as a potential noninvasive diagnostic specimen in clinical environments. Considerable research has been conducted to investigate ...
Source: Experimental Eye Research - April 28, 2024 Category: Opthalmology Authors: Keziah Mary Thomas Sreeprasad Ajithaprasad Mithun N Sanoop Pavithran Santhosh Chidangil Jijo Lukose Source Type: research
Experimental interventions attenuate a conjunctival epidermal metaplasia model
Exp Eye Res. 2024 Apr 26:109916. doi: 10.1016/j.exer.2024.109916. Online ahead of print.ABSTRACTThe conjunctiva is a non-keratinized, stratified columnar epithelium with characteristics different from the cornea and eyelid epidermis. From development to adulthood, a distinguishing feature of ocular versus epidermal epithelia is the expression of the master regulator PAX6. A conditionally immortalized conjunctival epithelial cell line (iHCjEC) devoid of stromal or immune cells established in our laboratory spontaneously manifested epidermal metaplasia and upregulated expression of the keratinization-related genes SPRR1A/B a...
Source: Experimental Eye Research - April 28, 2024 Category: Opthalmology Authors: Takezawa Yuki Masayoshi Kamon Keiko Hiraki-Kamon Arisa Mitani Atsushi Shiraishi Hidemasa Kato Source Type: research
Raman spectroscopy assisted tear analysis: A label free, optical approach for noninvasive disease diagnostics
Exp Eye Res. 2024 Apr 26:109913. doi: 10.1016/j.exer.2024.109913. Online ahead of print.ABSTRACTIn recent times, tear fluid analysis has garnered considerable attention in the field of biomarker- based diagnostics due to its noninvasive sample collection method. Tears encompass a reservoir of biomarkers that assist in diagnosing not only ocular disorders but also a diverse list of systemic diseases. This highlights the necessity for sensitive and dependable screening methods to employ tear fluid as a potential noninvasive diagnostic specimen in clinical environments. Considerable research has been conducted to investigate ...
Source: Experimental Eye Research - April 28, 2024 Category: Opthalmology Authors: Keziah Mary Thomas Sreeprasad Ajithaprasad Mithun N Sanoop Pavithran Santhosh Chidangil Jijo Lukose Source Type: research
Blast injury: Impact to the cornea
Exp Eye Res. 2024 Apr 25:109915. doi: 10.1016/j.exer.2024.109915. Online ahead of print.ABSTRACTVisual disorders are common even after mild traumatic brain injury (mTBI) or blast exposure. The cost of blast-induced vision loss in civilians, military personnel, and veterans is significant. The visual consequences of blasts associated with TBI are elusive. Active military personnel and veterans report various ocular pathologies including corneal disorders post-combat blasts. The wars and conflicts in Afghanistan, Iraq, Syria, Ukraine, etc. have increased the number of corneal and ocular disorders significantly among military...
Source: Experimental Eye Research - April 27, 2024 Category: Opthalmology Authors: Duraisamy Kempuraj Rajiv R Mohan Source Type: research
Blast injury: Impact to the cornea
Exp Eye Res. 2024 Apr 25:109915. doi: 10.1016/j.exer.2024.109915. Online ahead of print.ABSTRACTVisual disorders are common even after mild traumatic brain injury (mTBI) or blast exposure. The cost of blast-induced vision loss in civilians, military personnel, and veterans is significant. The visual consequences of blasts associated with TBI are elusive. Active military personnel and veterans report various ocular pathologies including corneal disorders post-combat blasts. The wars and conflicts in Afghanistan, Iraq, Syria, Ukraine, etc. have increased the number of corneal and ocular disorders significantly among military...
Source: Experimental Eye Research - April 27, 2024 Category: Opthalmology Authors: Duraisamy Kempuraj Rajiv R Mohan Source Type: research
Exosomal lncRNA-MIAT promotes neovascularization via the miR-133a-3p/MMP-X1 axis in diabetic retinopathy
Exp Eye Res. 2024 Apr 24:109912. doi: 10.1016/j.exer.2024.109912. Online ahead of print.ABSTRACTDiabetic retinopathy (DR), a most common microangiopathy of diabetes, causes vision loss and even blindness. The mechanisms of exosomal lncRNA remain unclear in the development of DR. Here, we first identifed the pro-angiogenic effect of exosomes derived from vitreous humor of proliferative diabetic retinopathy patients, where lncRNA-MIAT was enriched inside. Secondly, lncRNA-MIAT was demonstrated significantly increased in exosomes from high glucose induced human retinal vascular endothelial cell, and can regulate tube formatio...
Source: Experimental Eye Research - April 26, 2024 Category: Opthalmology Authors: Xinsheng Li Qiuchen Cao Changlin Xu Jinfan Wang Ting Pan Qinghuai Liu Ping Xie Zizhong Hu Source Type: research
Phospholipid Analyses of Rabbit Ocular Surface Tissues
This study (1) establishes PL profiles of the two ocular tissues of the integument that cover the surface of the eye, (2) compares and contrasts indices comprised of ratios and combinations of PL, and (3) describes pathway-specific metabolic interrelations among these tissues to serve as baselines for studies involving the distribution of tissue phospholipids.PMID:38663719 | DOI:10.1016/j.exer.2024.109911 (Source: Experimental Eye Research)
Source: Experimental Eye Research - April 25, 2024 Category: Opthalmology Authors: Jack V Greiner Thomas Glonek Source Type: research
Effects of Fluorescent Protein tdTomato on Mouse Retina
In this study, we discovered that tdTomato expression led to unexpected retinal dysfunction and ultrastructural defects in the transgenic mouse retina. The retinal dysfunction mainly manifested in the reduced photopic electroretinogram (ERG) responses and decreased contrast sensitivity in visual acuity, caused by mitochondrial damages characterized with cellular redistribution, morphological modifications and molecular profiling alterations. Taken together, our findings for the first time demonstrated the retinal dysfunction and ultrastructural defects in the retinas of tdTomato-transgenic mice, calling for a more careful ...
Source: Experimental Eye Research - April 25, 2024 Category: Opthalmology Authors: Chang-Jun Zhang Hao Mou Jing Yuan Ya-Han Wang Shu-Ning Sun Wen Wang Ze-Hua Xu Si-Jian Yu Kang-Xin Jin Zi-Bing Jin Source Type: research
Phospholipid Analyses of Rabbit Ocular Surface Tissues
This study (1) establishes PL profiles of the two ocular tissues of the integument that cover the surface of the eye, (2) compares and contrasts indices comprised of ratios and combinations of PL, and (3) describes pathway-specific metabolic interrelations among these tissues to serve as baselines for studies involving the distribution of tissue phospholipids.PMID:38663719 | DOI:10.1016/j.exer.2024.109911 (Source: Experimental Eye Research)
Source: Experimental Eye Research - April 25, 2024 Category: Opthalmology Authors: Jack V Greiner Thomas Glonek Source Type: research
Effects of Fluorescent Protein tdTomato on Mouse Retina
In this study, we discovered that tdTomato expression led to unexpected retinal dysfunction and ultrastructural defects in the transgenic mouse retina. The retinal dysfunction mainly manifested in the reduced photopic electroretinogram (ERG) responses and decreased contrast sensitivity in visual acuity, caused by mitochondrial damages characterized with cellular redistribution, morphological modifications and molecular profiling alterations. Taken together, our findings for the first time demonstrated the retinal dysfunction and ultrastructural defects in the retinas of tdTomato-transgenic mice, calling for a more careful ...
Source: Experimental Eye Research - April 25, 2024 Category: Opthalmology Authors: Chang-Jun Zhang Hao Mou Jing Yuan Ya-Han Wang Shu-Ning Sun Wen Wang Ze-Hua Xu Si-Jian Yu Kang-Xin Jin Zi-Bing Jin Source Type: research
