The porcine skin microbiome exhibits broad fungal antagonism
In this study, using the Wisconsin Miniature Swine™ model, we characterize the porcine skin fungal and bacterial microbiomes, identify bacterial isolates displaying antifungal activity, and use whole-genome sequencing to identify biosynthetic gene clusters encoding for secondary metabolites that may be responsible for the antagonistic effects on fungi. Through this comprehensive approach of paired microbiome sequencing with culturomics, we report the discovery of novel species of Corynebacterium and Rothia. Further, this study represents the first comprehensive evaluation of the porcine skin mycobiome and the evaluation ...
Source: Fungal Genetics and Biology - May 30, 2024 Category: Biology Authors: Karinda F De La Cruz Elizabeth C Townsend J Z Alex Cheong Rauf Salamzade Aiping Liu Shelby Sandstrom Evelin Davila Lynda Huang Kayla H Xu Sherrie Y Wu Jennifer J Meudt Dhanansayan Shanmuganayagam Angela L F Gibson Lindsay R Kalan Source Type: research
The porcine skin microbiome exhibits broad fungal antagonism
In this study, using the Wisconsin Miniature Swine™ model, we characterize the porcine skin fungal and bacterial microbiomes, identify bacterial isolates displaying antifungal activity, and use whole-genome sequencing to identify biosynthetic gene clusters encoding for secondary metabolites that may be responsible for the antagonistic effects on fungi. Through this comprehensive approach of paired microbiome sequencing with culturomics, we report the discovery of novel species of Corynebacterium and Rothia. Further, this study represents the first comprehensive evaluation of the porcine skin mycobiome and the evaluation ...
Source: Fungal Genetics and Biology - May 30, 2024 Category: Biology Authors: Karinda F De La Cruz Elizabeth C Townsend J Z Alex Cheong Rauf Salamzade Aiping Liu Shelby Sandstrom Evelin Davila Lynda Huang Kayla H Xu Sherrie Y Wu Jennifer J Meudt Dhanansayan Shanmuganayagam Angela L F Gibson Lindsay R Kalan Source Type: research
The porcine skin microbiome exhibits broad fungal antagonism
In this study, using the Wisconsin Miniature Swine™ model, we characterize the porcine skin fungal and bacterial microbiomes, identify bacterial isolates displaying antifungal activity, and use whole-genome sequencing to identify biosynthetic gene clusters encoding for secondary metabolites that may be responsible for the antagonistic effects on fungi. Through this comprehensive approach of paired microbiome sequencing with culturomics, we report the discovery of novel species of Corynebacterium and Rothia. Further, this study represents the first comprehensive evaluation of the porcine skin mycobiome and the evaluation ...
Source: Fungal Genetics and Biology - May 30, 2024 Category: Biology Authors: Karinda F De La Cruz Elizabeth C Townsend J Z Alex Cheong Rauf Salamzade Aiping Liu Shelby Sandstrom Evelin Davila Lynda Huang Kayla H Xu Sherrie Y Wu Jennifer J Meudt Dhanansayan Shanmuganayagam Angela L F Gibson Lindsay R Kalan Source Type: research
The porcine skin microbiome exhibits broad fungal antagonism
In this study, using the Wisconsin Miniature Swine™ model, we characterize the porcine skin fungal and bacterial microbiomes, identify bacterial isolates displaying antifungal activity, and use whole-genome sequencing to identify biosynthetic gene clusters encoding for secondary metabolites that may be responsible for the antagonistic effects on fungi. Through this comprehensive approach of paired microbiome sequencing with culturomics, we report the discovery of novel species of Corynebacterium and Rothia. Further, this study represents the first comprehensive evaluation of the porcine skin mycobiome and the evaluation ...
Source: Fungal Genetics and Biology - May 30, 2024 Category: Biology Authors: Karinda F De La Cruz Elizabeth C Townsend J Z Alex Cheong Rauf Salamzade Aiping Liu Shelby Sandstrom Evelin Davila Lynda Huang Kayla H Xu Sherrie Y Wu Jennifer J Meudt Dhanansayan Shanmuganayagam Angela L F Gibson Lindsay R Kalan Source Type: research
The porcine skin microbiome exhibits broad fungal antagonism
In this study, using the Wisconsin Miniature Swine™ model, we characterize the porcine skin fungal and bacterial microbiomes, identify bacterial isolates displaying antifungal activity, and use whole-genome sequencing to identify biosynthetic gene clusters encoding for secondary metabolites that may be responsible for the antagonistic effects on fungi. Through this comprehensive approach of paired microbiome sequencing with culturomics, we report the discovery of novel species of Corynebacterium and Rothia. Further, this study represents the first comprehensive evaluation of the porcine skin mycobiome and the evaluation ...
Source: Fungal Genetics and Biology - May 30, 2024 Category: Biology Authors: Karinda F De La Cruz Elizabeth C Townsend J Z Alex Cheong Rauf Salamzade Aiping Liu Shelby Sandstrom Evelin Davila Lynda Huang Kayla H Xu Sherrie Y Wu Jennifer J Meudt Dhanansayan Shanmuganayagam Angela L F Gibson Lindsay R Kalan Source Type: research
Expression of endogenous UDP-glucosyltransferase in endophyte Phomopsis liquidambaris reduces deoxynivalenol contamination in wheat
This study aimed to expand the genetic tools of the endophyte P. liquidambaris against Fusarium graminearum. Specifically, we engineered a UDP-glucosyltransferase-expressing P. liquidambaris strain (PL-UGT) using ADE1 as a selection marker and obtained a deletion mutant using an inducible promoter that drives Cas9 expression. Our PL-UGT strain converted deoxynivalenol (DON) into DON-3-G in vitro at a rate of 71.4 % after 36 h. DON inactivation can be used to confer tolerance in planta. Wheat seedlings inoculated with endophytic strain PL-UGT showed improved growth compared with those inoculated with wildtype P. liquidambar...
Source: Fungal Genetics and Biology - May 27, 2024 Category: Biology Authors: Meng-Qian Zhang Zhi Yang Yu-Xin Dong Ya-Li Zhu Xin-Yi Chen Chuan-Chao Dai Zhan Zhichun Yan-Zhen Mei Source Type: research
Expression of endogenous UDP-glucosyltransferase in endophyte Phomopsis liquidambaris reduces deoxynivalenol contamination in wheat
This study aimed to expand the genetic tools of the endophyte P. liquidambaris against Fusarium graminearum. Specifically, we engineered a UDP-glucosyltransferase-expressing P. liquidambaris strain (PL-UGT) using ADE1 as a selection marker and obtained a deletion mutant using an inducible promoter that drives Cas9 expression. Our PL-UGT strain converted deoxynivalenol (DON) into DON-3-G in vitro at a rate of 71.4 % after 36 h. DON inactivation can be used to confer tolerance in planta. Wheat seedlings inoculated with endophytic strain PL-UGT showed improved growth compared with those inoculated with wildtype P. liquidambar...
Source: Fungal Genetics and Biology - May 27, 2024 Category: Biology Authors: Meng-Qian Zhang Zhi Yang Yu-Xin Dong Ya-Li Zhu Xin-Yi Chen Chuan-Chao Dai Zhan Zhichun Yan-Zhen Mei Source Type: research
Expression of endogenous UDP-glucosyltransferase in endophyte Phomopsis liquidambaris reduces deoxynivalenol contamination in wheat
This study aimed to expand the genetic tools of the endophyte P. liquidambaris against Fusarium graminearum. Specifically, we engineered a UDP-glucosyltransferase-expressing P. liquidambaris strain (PL-UGT) using ADE1 as a selection marker and obtained a deletion mutant using an inducible promoter that drives Cas9 expression. Our PL-UGT strain converted deoxynivalenol (DON) into DON-3-G in vitro at a rate of 71.4 % after 36 h. DON inactivation can be used to confer tolerance in planta. Wheat seedlings inoculated with endophytic strain PL-UGT showed improved growth compared with those inoculated with wildtype P. liquidambar...
Source: Fungal Genetics and Biology - May 27, 2024 Category: Biology Authors: Meng-Qian Zhang Zhi Yang Yu-Xin Dong Ya-Li Zhu Xin-Yi Chen Chuan-Chao Dai Zhan Zhichun Yan-Zhen Mei Source Type: research
Anaerobic fungi contain abundant, diverse, and transcriptionally active Long Terminal Repeat retrotransposons
We report here the abundance and transcriptional activity of LTR retrotransposons across several species of the early-branching Neocallimastigomycota, otherwise known as the anaerobic gut fungi (AGF). The ubiquity of LTR retrotransposons in these genomes suggests key evolutionary roles in these rumen-dwelling biomass degraders, whose genomes also contain many enzymes that are horizontally transferred from other rumen-dwelling prokaryotes. Up to 10% of anaerobic fungal genomes consist of LTR retrotransposons, and the mapping of sequences from LTR retrotransposons to transcriptomes shows that the majority of clusters are tra...
Source: Fungal Genetics and Biology - May 16, 2024 Category: Biology Authors: Tejas A Navaratna Nabil Alansari Amy R Eisenberg Michelle A O'Malley Source Type: research
Anaerobic fungi contain abundant, diverse, and transcriptionally active Long Terminal Repeat retrotransposons
We report here the abundance and transcriptional activity of LTR retrotransposons across several species of the early-branching Neocallimastigomycota, otherwise known as the anaerobic gut fungi (AGF). The ubiquity of LTR retrotransposons in these genomes suggests key evolutionary roles in these rumen-dwelling biomass degraders, whose genomes also contain many enzymes that are horizontally transferred from other rumen-dwelling prokaryotes. Up to 10% of anaerobic fungal genomes consist of LTR retrotransposons, and the mapping of sequences from LTR retrotransposons to transcriptomes shows that the majority of clusters are tra...
Source: Fungal Genetics and Biology - May 16, 2024 Category: Biology Authors: Tejas A Navaratna Nabil Alansari Amy R Eisenberg Michelle A O'Malley Source Type: research
Anaerobic fungi contain abundant, diverse, and transcriptionally active Long Terminal Repeat retrotransposons
We report here the abundance and transcriptional activity of LTR retrotransposons across several species of the early-branching Neocallimastigomycota, otherwise known as the anaerobic gut fungi (AGF). The ubiquity of LTR retrotransposons in these genomes suggests key evolutionary roles in these rumen-dwelling biomass degraders, whose genomes also contain many enzymes that are horizontally transferred from other rumen-dwelling prokaryotes. Up to 10% of anaerobic fungal genomes consist of LTR retrotransposons, and the mapping of sequences from LTR retrotransposons to transcriptomes shows that the majority of clusters are tra...
Source: Fungal Genetics and Biology - May 16, 2024 Category: Biology Authors: Tejas A Navaratna Nabil Alansari Amy R Eisenberg Michelle A O'Malley Source Type: research
Anaerobic fungi contain abundant, diverse, and transcriptionally active Long Terminal Repeat retrotransposons
We report here the abundance and transcriptional activity of LTR retrotransposons across several species of the early-branching Neocallimastigomycota, otherwise known as the anaerobic gut fungi (AGF). The ubiquity of LTR retrotransposons in these genomes suggests key evolutionary roles in these rumen-dwelling biomass degraders, whose genomes also contain many enzymes that are horizontally transferred from other rumen-dwelling prokaryotes. Up to 10% of anaerobic fungal genomes consist of LTR retrotransposons, and the mapping of sequences from LTR retrotransposons to transcriptomes shows that the majority of clusters are tra...
Source: Fungal Genetics and Biology - May 16, 2024 Category: Biology Authors: Tejas A Navaratna Nabil Alansari Amy R Eisenberg Michelle A O'Malley Source Type: research
Anaerobic fungi contain abundant, diverse, and transcriptionally active Long Terminal Repeat retrotransposons
We report here the abundance and transcriptional activity of LTR retrotransposons across several species of the early-branching Neocallimastigomycota, otherwise known as the anaerobic gut fungi (AGF). The ubiquity of LTR retrotransposons in these genomes suggests key evolutionary roles in these rumen-dwelling biomass degraders, whose genomes also contain many enzymes that are horizontally transferred from other rumen-dwelling prokaryotes. Up to 10% of anaerobic fungal genomes consist of LTR retrotransposons, and the mapping of sequences from LTR retrotransposons to transcriptomes shows that the majority of clusters are tra...
Source: Fungal Genetics and Biology - May 16, 2024 Category: Biology Authors: Tejas A Navaratna Nabil Alansari Amy R Eisenberg Michelle A O'Malley Source Type: research
Anaerobic fungi contain abundant, diverse, and transcriptionally active Long Terminal Repeat retrotransposons
We report here the abundance and transcriptional activity of LTR retrotransposons across several species of the early-branching Neocallimastigomycota, otherwise known as the anaerobic gut fungi (AGF). The ubiquity of LTR retrotransposons in these genomes suggests key evolutionary roles in these rumen-dwelling biomass degraders, whose genomes also contain many enzymes that are horizontally transferred from other rumen-dwelling prokaryotes. Up to 10% of anaerobic fungal genomes consist of LTR retrotransposons, and the mapping of sequences from LTR retrotransposons to transcriptomes shows that the majority of clusters are tra...
Source: Fungal Genetics and Biology - May 16, 2024 Category: Biology Authors: Tejas A Navaratna Nabil Alansari Amy R Eisenberg Michelle A O'Malley Source Type: research
Anaerobic fungi contain abundant, diverse, and transcriptionally active Long Terminal Repeat retrotransposons
We report here the abundance and transcriptional activity of LTR retrotransposons across several species of the early-branching Neocallimastigomycota, otherwise known as the anaerobic gut fungi (AGF). The ubiquity of LTR retrotransposons in these genomes suggests key evolutionary roles in these rumen-dwelling biomass degraders, whose genomes also contain many enzymes that are horizontally transferred from other rumen-dwelling prokaryotes. Up to 10% of anaerobic fungal genomes consist of LTR retrotransposons, and the mapping of sequences from LTR retrotransposons to transcriptomes shows that the majority of clusters are tra...
Source: Fungal Genetics and Biology - May 16, 2024 Category: Biology Authors: Tejas A Navaratna Nabil Alansari Amy R Eisenberg Michelle A O'Malley Source Type: research
