P –084 Microfluidic Sperm Sorting (MFSS) technique versus Physiological Intracytoplasmic Sperm Injection (PICSI) technique in high DNA fragmentation index sperm samples
AbstractStudy questionTo evaluate the effectiveness of using Microfluidic Sperm Sorting (MFSS) technique and Physiological Intracytoplasmic Sperm Injection (PICSI) technique in patient with high DNA fragmentation index (DFI) sperm samples.Summary answerSperm selected by microfluidic sorting are associated with significant increase in day 3 grade A embryo development rate, clinical pregnancy rate over PICSI.What is known alreadyDNA damage is unrecognisable in living sperm prior to insemination and an increased sperm DNA fragmentation index has been associated with lower fertilization rates, impaired embryo development and r...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –083 Analysis of chromosomal segregation and interchromosomal effects (ICE) in sperms from balanced translocation carriers using fluorescence in situ hybridization (FISH) after sperm selector separation
AbstractStudy questionInfluence of sperm selector separation of sperms on their translocation load, segregation pattern, motility and occurrence of interchromosomal effectsSummary answerSperm selector separation led to reduction of the translocation load, shift in segregation pattern and lower rates of interchromosomal effects within sperm samplesWhat is known alreadyBalanced translocations in men are known to be one of the main causes of reproductive failure. The segregation pattern in sperms is determined by the distribution of the chromosomes during meiosis. Interchromosomal effects can also influence the distribution o...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –082 Effect of semen hyper viscosity (SHV) on blastocyst formation rate and implantation rate
AbstractStudy questionDoes semen hyper viscosity effects blastocyst formation rateSummary answerHyper viscosity of semen sample later results in poor blastocyst formation rate and lower implantation rate.What is known alreadyNormal range of semen hyper viscosity ranges between 12 –29%.Highly viscous semen samples impairs the physical and chemical characteristics of seminal fluid and due to which seminal oxidative damage increases which further increases the ROS and reduces the sperm motility there are some factors that can affect the seminal viscosity out of which one is M ale accessory gland infection, Hypo function of ...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –081 Microfluidic sorting does not improve clinical outcomes compared to magnetic activated cell sorting (MACS) in Assisted Reproduction
This study has not considered the indication in male factor couples due to a high degree of double-strand DNA fragmentation. Therefore, more specific studies are required to determine in which patients, microfluidics sorter selection would significantly improve clinical outcomes.Wider implications of the findings: In an unselected population, magnetic activated cell sorting significantly improves clinical outcomes compared to a microfluidic technique, so this latter method should not be recommended without a male factor indication associated with sperm DNA damage. The proposed microfluidic technology does not seem to offer...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –080 Higher sperm DNA fragmentation reduces the proportion of good quality embryos at day 5 on IVF and ICSI cycles from unselected males
In this study, a higher amount of data was compiled so that a large number of embryos were analyzed. The DNA integrity of the sperm may be an important consideration when poor quality embryos were obtained in the previous cycle when deciding on the next clinical strategy to apply.Trial registration numberNA (Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –079 A spontaneous LH peak before triggering for intrauterine insemination with donor sperm (IUI-D) is associated to lower live birth rates
AbstractStudy questionAre live birth rates after IUI with donor sperm (IUI-D) and controlled ovarian stimulation comparable between women with a spontaneous LH peak vs those without?Summary answerBiochemical, clinical, ongoing pregnancy rates and live birth rates were higher among women without an LH peak.What is known alreadyIt is common clinical practice to trigger ovulation in IUI cycles once specific criteria are met; if a natural LH surge appears, adjusting the IUI timing may become necessary. Pregnancy rates seem to be slightly better when IUI is scheduled in relation to the presence or absence of an LH peak in non-s...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
P –078 Prognostic factors for male fertility recovery after microsurgical varicocelectomy
AbstractStudy questionWhat clinical and laboratory parameters are reliable predictors of spontaneous pregnancy (SP) after microsurgical varicocelectomy in men from infertile couples?Summary answerPredictors of SP after microsurgical varicocele repair are the male age, baseline total sperm motility, and postoperative increase level of TPMSC.What is known alreadyVaricocele is the most common correctable cause of male subfertility. According to the recent meta-analyses and studies, microsurgical varicocelectomy is the “golden standard” method for varicocele repair. However, it is still unclear why at least one-third of su...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-236 The clinical and morphokinetic factors indicating a risk of pregnancy loss after a euploid embryo transfer
AbstractStudy questionAre there any clinical and morphokinetic factors which may affect the pregnancy outcome after a euploid embryo transfer?Summary answerBody mass index (BMI), endometriosis, the history of recurrent pregnancy losses and the number of previous frozen-thawed unsuccessful embryo transfer (FET) cycles impact pregnancy outcomes.What is known alreadyPreimplantation genetic testing for aneuploidy (PGT-A) is largely used for various indications to detect chromosomal abnormalities in assisted reproductive technologies (ART). The most common reason for the first trimester pregnancy losses is chromosomal abnormali...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-235 ERICA (Embryo Ranking Intelligent Classification Assistant) AI predicts miscarriage in poorly ranked embryos from one static, non-invasive embryo image assessment
AbstractStudy questionDoes ERICA ’s prognosis ranking based on ploidy, predict early miscarriage following positive biochemical pregnancy test?Summary answerThe lower ERICA grades embryos, the higher the likelihood of early miscarriage, irrespective of age group.What is known alreadyThe vast majority of early miscarriages are due to aneuploidy, but preimplantation genetic testing for aneuploidy (PGTA) is potentially invasive, expensive, time-consuming and usually necessitates cryopreservation. Current methods for embryo selection based on morphology and morphokinetics are poorly correlated with ploidy. ERICA is a deep-le...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-234 Using serum metabolomics to identify biomarkers of viable early, intrauterine pregnancy: an untargeted 1H NMR-based approach
This study identifies a metabolite profile associated with VIUPs. These findings may be useful in the development of a diagnostic test to confirm VIUPs and thus exclude potentially life-threatening pregnancy outcomes. Such a test would be invaluable in clinical emergencies.Trial registration numberNA (Source: Human Reproduction)
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-233 Micronized progesterone plus dydrogesterone versus micronized progesterone alone for luteal phase support in frozen-thawed cycles: a prospective cohort study
AbstractStudy questionDoes the addition of oral dydrogesterone to vaginal progesterone as luteal phase support improve pregnancy outcomes during frozen embryo transfer (FET) cycles compared with vaginal progesterone alone?Summary answerLuteal phase support with oral dydrogesterone added to vaginal progesterone improves live birth rates and reduces miscarriage rates compared with vaginal progesterone alone.What is known alreadyProgesterone is an important hormone that triggers secretory transformation of the endometrium to allow implantation of the embryo. During in vitro fertilization (IVF), exogenous progesterone is admin...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-232 Higher clinical pregnancy rate after oxytocin-receptor antagonist administration around the time of embryo transfer: A systematic review and meta-analysis of eleven RCTs
AbstractStudy questionDoes the administration of an oxytocin-receptor antagonist around time of embryo transfer in IVF impact the likelihood to achieve a clinical pregnancy?Summary answerAdministration of oxytocin-receptor antagonists around embryo transfer increases the likelihood of clinical pregnancy achievement.What is known alreadyUterine contractions occurring around time of embryo transfer have been described as one possible mechanism of failure of implantation of an embryo in the context of in-vitro fertilization (IVF). Hence the utilization of oxytocin-receptor antagonists was evaluated in randomized clinical tria...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-231 In vitro maturation of human immature (GV) oocytes after controlled ovarian hormonal stimulation with recombinant AMH in the maturation medium
AbstractStudy questionDoes the addition of recombinant AMH to thein vitro maturation (IVM) medium improve the maturation of GV oocytes after controlled ovarian hormonal stimulation?Summary answerOur results show that the addition of recombinant AMH to thein vitro maturation medium improves the maturation rate of GV oocytes.What is known alreadyAnti-M üllerian hormone (AMH) is an important hormone involved in the process of sex differentiation during embryonic development. At the transition to the 21. century, more and more researchers have studied the role of AMH in ovarian function, especially its impact on folliculogene...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-230 RCT comparing Recombinant-hcg trigger with Dual-trigger (GnRH-agonist and recombinant-hcg ) in improving clinical outcome in ICSI cycles in women with Diminished Ovarian Reserve
AbstractStudy questionDoes use of Dual-trigger (GnRH-agonist with recombinant-hcg) improve the clinical outcome in women with diminished ovarian reserve as compared to Recombinant-hcg trigger?Summary answerYes, the use of Dual-trigger (GnRH-agonist with recombinant-hcg) improve the clinical outcome in women with diminished ovarian reserve as compared to Recombinant-hcg trigger.What is known alreadyThe population of poor responders has grown exponentially over the years and their management of ovarian stimulation remains one of the most challenging aspects. In GnRH antagonist down-regulated IVF-ICSI cycles, dual triggering ...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
O-229 Impact of letrozole co-treatment during ovarian stimulation with gonadotropins for in vitro fertilisation (IVF): a multicentre, randomised, double-blinded placebo-controlled trial
AbstractStudy questionDoes reducing estradiol levels with letrozole co-treatment during ovarian stimulation with gonadotropins for IVF impact endocrinological and reproductive outcome markers in expected normal responders?Summary answerLetrozole co-treatment maintained follicular phase physiological serum estradiol levels, increased gonadotropin and androgen levels, and increased progesterone in the luteal phase.What is known alreadyOvarian stimulation for IVF causes supraphysiologic estradiol levels, which exert pituitary suppression reducing gonadotropin stimulation of the corpus luteum. Furthermore, stimulation may incr...
Source: Human Reproduction - August 6, 2021 Category: Reproduction Medicine Source Type: research
