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Ginsenoside Rg1 Induces Autophagy in Colorectal Cancer through Inhibition of the Akt/mTOR/p70S6K Pathway
This study aimed to elucidate the anti-colon cancer mechanism of ginsenoside Rg1 in vitro and in vivo. Cell viability rate was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tetrazolium assay. The inhibitory effect of ginsenoside Rg1 against CT26 cell proliferation gradually increased with increasing concentration. The in vivo experiments also demonstrated an antitumor effect. The monodansylcadaverine (MDC), transmission electron microscopy (TEM), and expression of autophagy marker proteins confirmed that ginsenoside Rg1 induced autophagy in vitro. Ginsenoside Rg1 induced autophagy de...
Source: Journal of Microbiology and Biotechnology - April 26, 2024 Category: Biotechnology Authors: Ruiqi Liu Bin Zhang Shuting Zou Li Cui Lin Lin Lingchang Li Source Type: research

Efficacy Assessment of the Co-Administration of Vancomycin and Metronidazole in Clostridioides difficile -Infected Mice Based on Changes in Intestinal Ecology
In this study, a CDI mouse model was constructed to evaluate the therapeutic effects of VAN and MTR alone or in combination. For a perspective on the intestinal ecology, 16S rRNA amplicon sequencing and non-targeted metabolomics techniques were used to investigate changes in the fecal microbiota and metabolome of mice under the co-administration treatment. As a result, the survival rate of mice under co-administration was not dramatically different compared to that of single antibiotics, and the former caused intestinal tissue hyperplasia and edema. Co-administration also significantly enhanced the activity of amino acid m...
Source: Journal of Microbiology and Biotechnology - April 26, 2024 Category: Biotechnology Authors: Saiwei Zhong Jingpeng Yang He Huang Source Type: research

Corrigendum to: Low Molecular Weight Collagen Peptide (LMWCP) Promotes Hair Growth by Activating the Wnt/GSK-3 β/β-Catenin Signaling Pathway
J Microbiol Biotechnol. 2024 Apr 28;34(4):985-986. doi: 10.4014/jmb.2024.3404.985.NO ABSTRACTPMID:38668686 | DOI:10.4014/jmb.2024.3404.985 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - April 26, 2024 Category: Biotechnology Authors: Yujin Kim Jung Ok Lee Jung Min Lee Mun-Hoe Lee Hyeong-Min Kim Hee-Chul Chung Do-Un Kim Jin-Hee Lee Beom Joon Kim Source Type: research

Inhibition of Verticillium Wilt in Cotton through the Application of Pseudomonas aeruginosa ZL6 Derived from Fermentation Residue of Kitchen Waste
J Microbiol Biotechnol. 2024 Apr 5;34(5):1-10. doi: 10.4014/jmb.2401.01022. Online ahead of print.ABSTRACTTo isolate and analyze bacteria with Verticillium wilt-resistant properties from the fermentation residue of kitchen wastes, as well as explore their potential for new applications of the residue. A total of six bacterial strains exhibiting Verticillium wilt-resistant capabilities were isolated from the biogas residue of kitchen waste fermentation. Using a polyphasic approach, strain ZL6, which displayed the highest antagonistic activity against cotton Verticillium wilt, was identified as belonging to the Pseudomonas a...
Source: Journal of Microbiology and Biotechnology - April 11, 2024 Category: Biotechnology Authors: Qiuhong Niu Shengwei Lei Guo Zhang Guohan Wu Zhuo Tian Keyan Chen Lin Zhang Source Type: research

In vivo Effects of Salicornia herbacea and Calystegia soldanella Extracts for Memory Improvement
J Microbiol Biotechnol. 2024 Mar 13;34(5):1-10. doi: 10.4014/jmb.2312.12045. Online ahead of print.ABSTRACTThe global elderly population, aged 65 and over, reached approximately 10% in 2020, and this proportion is expected to continue rising. Therefore, the prevalence of neurodegenerative diseases such as Parkinson's disease (PD), which are characterized by declining memory capabilities, is anticipated to increase. In a previous study, we successfully restored the diminished memory capabilities in a fruit fly model of PD by administering an omija extract. To identify functional ingredients that can enhance memory akin to t...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Jiun Sang Seeta Poudel Youngseok Lee Source Type: research

 Escherichia coli Cytoplasmic Expression of Disulfide-Bonded Proteins: Side-by-Side Comparison Between Two Competing Strategies
J Microbiol Biotechnol. 2024 Mar 29;34(5):1-10. doi: 10.4014/jmb.2311.11025. Online ahead of print.ABSTRACTThe production of disulfide bond-containing recombinant proteins in Escherichia coli has traditionally been done by either refolding from inclusion bodies or by targeting the protein to the periplasm. However, both approaches have limitations and were developed to allow the production of proteins with disulfide bonds in the cytoplasm of E. coli: i) engineered strains with deletions in the disulfide reduction pathways, e.g. SHuffle, and ii) the co-expression of oxidative folding catalysts, e.g. CyDisCo. However, to our...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Angel Castillo-Corujo Yuko Uchida Mirva J Saaranen Lloyd W Ruddock Source Type: research

Biosynthesis of apigenin glucosides in engineered Corynebacterium glutamicum 
J Microbiol Biotechnol. 2024 Mar 14;34(5):1-10. doi: 10.4014/jmb.2401.01017. Online ahead of print.ABSTRACTGlucosylation is a well-known approach to improve the solubility, pharmacological, and biological properties of flavonoids, making flavonoid glucosides a target for large-scale biosynthesis. However, the low yield of products coupled with the requirement of expensive UDP-sugars limits the application of enzymatic systems for large-scale. C. glutamicum is a Gram-positive and generally regarded as safe (GRAS) bacteria frequently employed for the large-scale production of amino acids and bio-fuels. Due to the versatility...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Obed Jackson Amoah Samir Bahadur Thapa Su Yeong Ma Hue Thi Nguyen Morshed Md Zakaria Jae Kyung Sohng Source Type: research

Construction of a new Agrobacterium tumefaciens -mediated transformation system based on a dual auxotrophic approach in Cordyceps militaris 
In this study, we constructed an ATMT system based on a dual pyrG and hisB auxotrophic mutant of C. militaris. Using the uridine/uracil auxotrophic mutant as the background and pyrG as a selection marker, the hisB gene encoding imidazole glycerophosphate dehydratase, required for histidine biosynthesis, was knocked out by homologous recombination to construct a histidine auxotrophic C. militaris mutant. Then, pyrG in the histidine auxotrophic mutant was deleted to construct a ΔpyrG ΔhisB dual auxotrophic mutant. Further, we established an ATMT transformation system based on the dual auxotrophic C. militaris by using GFP ...
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Huan Huan Yan Yi Tong Shang Li Hong Wang Xue Qin Tian Van-Tuan Tran Li Hua Yao Bin Zeng Zhi Hong Hu Source Type: research

Screening Plant Growth-Promoting Bacteria with Antimicrobial Properties for Upland Rice
In conclusion, the in vitro plant growth-promoting and disease-controlling attributes of Bacillus sp. KK281 make it a strong candidate for field evaluation to boost plant growth and manage disease in upland rice.PMID:38563101 | DOI:10.4014/jmb.2402.02008 (Source: Journal of Microbiology and Biotechnology)
Source: Journal of Microbiology and Biotechnology - April 2, 2024 Category: Biotechnology Authors: Khammool Khamsuk Bernard Dell Wasu Pathom-Aree Wanwarang Pathaichindachote Nungruthai Suphrom Nareeluk Nakaew Juangjun Jumpathong Source Type: research