Integrative informatics analysis identifies that ginsenoside Re improves renal fibrosis through regulation of autophagy
In this study, we conducted a meta-analysis and pathway enrichment analysis of all published transcriptome data to identify differentially expressed genes (DEGs) and pathways of G-Re treatment. We then performed in vitro studies to measure the identified autophagy and fibrosis markers in HK2 cells. In vivo studies were conducted using ureteric obstruction (UUO) and aristolochic acid nephropathy (AAN) models to evaluate the effects of G-Re on autophagy and kidney fibrosis. Our informatics analysis identified autophagy-related pathways enriched for G-Re treatment. Treatment with G-Re in HK2 cells reduced autophagy and mRNA l...
Source: Journal of Natural Medicines - April 29, 2024 Category: Drugs & Pharmacology Source Type: research
Polyphyllin I induces rapid ferroptosis in acute myeloid leukemia through simultaneous targeting PI3K/SREBP-1/SCD1 axis and triggering of lipid peroxidation
AbstractAcute myeloid leukemia (AML) is a malignant disease that is difficult to completely cure. Polyphyllin I (PPI), a steroidal saponin isolated fromParis polyphylla, has exhibited multiple biological activities. Here, we discovered the superior cytotoxicity of PPI on AML cells MOLM-13 with an IC50 values of 0.44 ± 0.09 μM. Mechanically, PPI could cause ferroptosis via the accumulation of intracellular iron concentration and triggering lipid peroxidation. Interestingly, PPI could induced stronger ferroptosis in a short time of about 6 h compared to erastin. Furthermore, we demonstrate that PPI-induc ed rapid fe...
Source: Journal of Natural Medicines - April 26, 2024 Category: Drugs & Pharmacology Source Type: research
Evaluation of Rhododendri Mollis Flos and its representative component as a potential analgesic
In conclusion, the chemical substances and biological effects ofR. mole Flos changed significantly before and after processing, and the representative component RJ-VI has the potential to be developed into an effective analgesic.Graphical abstract (Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - April 26, 2024 Category: Drugs & Pharmacology Source Type: research
Two new sesterterpenoids from Atractylodes japonica Koidz. ex Kitam
AbstractTwo new sesterterpenoids, atractylodes japonica terpenoid acid I (1) and atractylodes japonica terpenoid aldehyde I (2), were isolated from the rhizomes ofAtractylodes japonica Koidz. ex Kitam together with ten known compounds (3 –12). Their structures were elucidated on the basis of comprehensive spectroscopic analysis (1D/2D NMR, HRESIMS and IR). In addition, all of these isolated compounds were evaluated for their cytotoxic activities against human gastric cancer cell MGC-803 and human hepatocellular cancer cell HepG-2. Most of them exhibited moderate to weak inhibitory effects with IC50 values in the range of...
Source: Journal of Natural Medicines - April 25, 2024 Category: Drugs & Pharmacology Source Type: research
Promotion of neurite outgrowth by 3,5,7,3 ʹ,4ʹ-pentamethoxyflavone is mediated through ERK signaling pathway in Neuro2a cells
In this study, the effects of 3,5,7,3 ʹ,4ʹ-pentamethoxyflavone (KP1), a major bioactive ingredient isolated from theKaempferia parviflora rhizomes, on a neurite outgrowth in Neuro2a cells and its mechanism have been investigated. KP1 increased concentration-dependently the percentage of neurite-bearing cells. KP1 showed a remarkable capability to elicit neurite outgrowth in Neuro2a cells, as evidenced by morphological alterations and immunostaining using anti-class III β-tubulin and anti-NeuN antibodies. KP1 also displayed a higher neurogenic activity than retinoic acid (RA), a promoter of neurite outgrowth in Neuro2a c...
Source: Journal of Natural Medicines - April 25, 2024 Category: Drugs & Pharmacology Source Type: research
Ameliorative effect of bofutsushosan (Fangfengtongshengsan) extract on the progression of aging-induced obesity
This study aimed to compare fat accumulation in young and aged mice raised on a high-fat diet and to characterize the obesity-reducing effects of a Kampo medicine, bofutsushosan (BTS; fangfengtongshengsan in Chinese). Aged mice fed a high-fat diet containing 2% BTS extract for 28 days exhibited a significant reduction in weight gain and accumulation of visceral and subcutaneous fat, which were greater degree of reduction than those of the young mice. When the treatment period was extended to two months, the serum aspartate aminotransferase and alanine aminotransferase leve ls and the accumulation of fat droplets in the h...
Source: Journal of Natural Medicines - April 25, 2024 Category: Drugs & Pharmacology Source Type: research
New biflavonoids isolated from Xylia kerrii leaves extract with selective cytotoxicity against MH7A human rheumatoid arthritis synovial fibroblast cells
AbstractThree new biflavonoids (1–3) and two known flavonoids (4,5) were isolated fromXylia kerrii collected in Thailand. Compounds1–5 showed selective cytotoxicity against the rheumatoid fibroblast-like synovial MH7A cell line, and these compounds showed weak cytotoxicity against the human lung synovial fibroblast WI-38 VA13 sub 2 RA cell line. Notably, compound1 was highly selective toward MH7A cells with an IC50 value of 6.9 μM, whereas the IC50 value for WI-38 VA13 sub 2 RA cells was> 100 μM. The western blotting analysis of MH7A cells treated with compound1 showed increased CDKN2A /p16INK4A and caspase-...
Source: Journal of Natural Medicines - April 9, 2024 Category: Drugs & Pharmacology Source Type: research
Proposal for structure revision of pinofuranoxin A through total syntheses of stereoisomers
AbstractThe relative configuration of the epoxide functionality in pinofuranoxin A (1), α-alkylidene-β-hydroxy-γ-methyl-γ-butyrolactone withtrans-epoxy side chain isolated by Evidente et al. in 2021, was revised by DFT-based spectral reinvestigations and stereo-controlled synthesis. The present investigation demonstrates the difficulty of the configurational elucidation of the stereogenic centers on the conformationally flexible acyclic side-chains. Sharpless ’s enantioselective epoxidations and dihydroxylations were quite effective in the reinvestigations of the configurations. As our syntheses made all diastereomer...
Source: Journal of Natural Medicines - April 8, 2024 Category: Drugs & Pharmacology Source Type: research
Hypoglycemic effects of mountain caviar extract and inhibitory mechanism of saponins, including momordin Ic, on glucose absorption
AbstractMountain caviar is a fruit ofKochia scoparia that contains momordin Ic as a major saponin constituent. Its extract (MCE) has been shown to suppress blood glucose elevations in the human oral glucose tolerance test (OGTT) as well as increases in blood glucose in OGTT, gastric emptying (GE), and glucose incorporation in the small intestine in rats. However, the effects of MCE and momordin Ic on glucose absorption in mice and these action mechanisms have not been examined for more than 2 decades. Therefore, we herein investigated the effects of MCE, its saponin fraction, and momordin Ic on blood glucose elevations in ...
Source: Journal of Natural Medicines - April 8, 2024 Category: Drugs & Pharmacology Source Type: research
Alkylphthalides with intracellular triglyceride metabolism-promoting activity from the rhizomes of Cnidium officinale Makino
AbstractMethanol extract of theCnidium officinale Makino rhizome, which is used as a crude drug Cnidium Rhizome (Cnidii Rhizoma; “Senkyu” in Japanese) and is listed in the Japanese Pharmacopoeia XVIII, showed intracellular triglyceride metabolism-promoting activity in high glucose-pretreated HepG2 cells. Thirty-five constituents, including two new alkylphthalide glycosides, senkyunosides A (1) and B (2), and a neolignan with a new stereoisomeric structure (3), were isolated in the extract. Their stereostructures were elucidated based on chemical and spectroscopic evidence. Among the isolates, several alkylphthalides, (...
Source: Journal of Natural Medicines - April 4, 2024 Category: Drugs & Pharmacology Source Type: research
Cryptobuchanosides A –G: seven previously undescribed triterpene glycosides from Cryptolepis buchananii R.Br. ex Roem. and Schult. with nitric oxide production inhibition activity
In this study, nine triterpene glycosides including seven previously undescribed compounds (1–7), were isolated from leaves ofCryptolepis buchananii R.Br. ex Roem. and Schult. using various chromatographic methods. The chemical structures of the compounds were elucidated to be 3-O- β-d-glucopyranosyl-(1 → 6)-β-d-glucopyranosyluncargenin C 28-O- α-l-rhamnopyranosyl-(1 → 2)-β-d-glucopyranosyl ester (1), 3-O- β-d-glucopyranosyl-(1 → 2)-β-d-glucopyranosyluncargenin C 28-O- α-l-rhamnopyranosyl-(1 → 2)-β-d-glucopyranosyl ester (2), 3-O- β-d-glucopyranosyl-(1 → 2)-β-d-glucopyranosylu...
Source: Journal of Natural Medicines - April 4, 2024 Category: Drugs & Pharmacology Source Type: research
Compound –compound interaction analysis of baicalin and berberine derivatives in aqueous solution
AbstractBaicalin and berberine are biologically active constituents of the crude drugs Scutellaria root and Coptis rhizome/Phellodendron bark, respectively. Baicalin and berberine are reported to combine together as a 1:1 complex that forms yellow precipitates by electrostatic interaction in decoctions of Kampo formulae containing these crude drugs. However, the structural basis and mechanism for the precipitate formation of this compound –compound interaction in aqueous solution remains unclarified. Herein, we searched for berberine derivatives in the Coptis rhizome that interact with baicalin and identified the chemica...
Source: Journal of Natural Medicines - April 4, 2024 Category: Drugs & Pharmacology Source Type: research
Correction: Evaluation of rooting characteristics of Ephedra cuttings by anatomy and promising strain selection based on rooting characteristics and alkaloid content
(Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - April 3, 2024 Category: Drugs & Pharmacology Source Type: research
Correction: Heating or ginger extract reduces the content of Pinellia ternata lectin in the raphides of Pinellia tuber
(Source: Journal of Natural Medicines)
Source: Journal of Natural Medicines - April 3, 2024 Category: Drugs & Pharmacology Source Type: research
Oxomollugin, an oxidized substance in mollugin, inhibited LPS-induced NF- κB activation via the suppressive effects on essential activation factors of TLR4 signaling
In this study, we investigated the inhibitory activity of oxomollugin, focusing on TLR4 signaling pathway, resulting in NF-κB activation. Oxomollugin inhibited the LPS-induced association of essential factors for initial activation of TLR4 signaling, MyD88, IRAK4 and TRAF6. Furtherm ore, oxomollugin showed suppressive effects on LPS-induced modification of IRAK1, IRAK2 and TRAF6, LPS-induced association of TRAF6-TAK1/TAB2, and followed by IKKα/β phosphorylation, which critical in signal transduction leading to LPS-induced NF-κB activation. The consistent results suggested th at oxomollugin inhibits LPS-induced NF-κB a...
Source: Journal of Natural Medicines - April 2, 2024 Category: Drugs & Pharmacology Source Type: research
