Calpain 8 as a potential biomarker regulates the progression of pancreatic cancer via EMT and AKT/ERK pathway
J Proteomics. 2024 Apr 30:105182. doi: 10.1016/j.jprot.2024.105182. Online ahead of print.ABSTRACTCalpain is a non-lysozyme, calcium-dependent intracellular cysteine protease that has been shown to play a role in tumor proliferation, survival, migration, invasion, and apoptosis. Dysregulation of calpain expression is closely related to tumorigenesis. However, the role of calpain-8 (CAPN8), as a member of the calpain family, in pancreatic cancer (PC) is remains unclear. In elucidating the mechanism of CAPN8 in PC, a comprehensive bioinformatics analysis and in vitro experiments were conducted. The TCGA database was used to ...
Source: Journal of Proteomics - May 2, 2024 Category: Biochemistry Authors: Na Song Kai Cui Liqun Zeng Yanwu Fan Ziwei Wang Pingyu Shi Wei Su Haijun Wang Source Type: research
Comparative proteomics analysis of samples from hepatic cystic echinococcosis patients using data-independent acquisition approach
This study aimed to assess the specific proteins that are potential candidates for the development of a vaccine against E. granulosus. The data-independent acquisition approach was employed to identify differentially expressed proteins (DEPs) in E. granulosus samples. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was employed to identify several noteworthy proteins. Results: The DEPs in E. granulosus samples were identified (245 pericystic wall vs. parasite-free yellowish granuloma (PYG, 1725 PY vs. PYG, 2274 PN vs. PYG). Further examination of these distinct proteins...
Source: Journal of Proteomics - May 2, 2024 Category: Biochemistry Authors: Kahaer Tuerxun Rong-Hua Tang Aabudouxikuer Abudoumijiti Zainuer Yusupu Aizemaiti Aikebaier Salamu Mijiti Irshat Ibrahim Yan-Long Cao Abudoukeyimu Yasheng Yuan-Quan Wu Source Type: research
Integrative proteomic and transcriptomic analysis in the female goat ovary to explore the onset of puberty
This study conducted the first transcriptomic and proteomic correlation analysis of pre-pubertal and pubertal goat ovaries and identified six significantly correlated molecules at both the gene and protein levels. Meanwhile, we were drawn to several molecules and signalling pathways that may play a regulatory role in the onset of puberty and reproduction by influencing reproductive-related gene expression, GnRH receptor signals, energy metabolism, ovarian hormone secretion, follicular development, and ovulation. This information contributed to identify potential biomarkers in pubertal goat ovaries, which was vital for pred...
Source: Journal of Proteomics - April 30, 2024 Category: Biochemistry Authors: Ping Qin Zhihao Pan Wei Zhang Rui Wang Xiaoqian Li Juntai Lu Shuangshuang Xu Xinbao Gong Jing Ye Xu Yan Ya Liu Yunsheng Li Yunhai Zhang Fugui Fang Source Type: research
Integrative proteomic and transcriptomic analysis in the female goat ovary to explore the onset of puberty
This study conducted the first transcriptomic and proteomic correlation analysis of pre-pubertal and pubertal goat ovaries and identified six significantly correlated molecules at both the gene and protein levels. Meanwhile, we were drawn to several molecules and signalling pathways that may play a regulatory role in the onset of puberty and reproduction by influencing reproductive-related gene expression, GnRH receptor signals, energy metabolism, ovarian hormone secretion, follicular development, and ovulation. This information contributed to identify potential biomarkers in pubertal goat ovaries, which was vital for pred...
Source: Journal of Proteomics - April 30, 2024 Category: Biochemistry Authors: Ping Qin Zhihao Pan Wei Zhang Rui Wang Xiaoqian Li Juntai Lu Shuangshuang Xu Xinbao Gong Jing Ye Xu Yan Ya Liu Yunsheng Li Yunhai Zhang Fugui Fang Source Type: research
Proteomic analysis revealed that the oomyceticide phosphite exhibits multi-modal action in an oomycete pathosystem
J Proteomics. 2024 Apr 24:105181. doi: 10.1016/j.jprot.2024.105181. Online ahead of print.ABSTRACTPhytopathogenic oomycetes constitute some of the most devastating plant pathogens and cause significant crop and horticultural yield and economic losses. The phytopathogen Phytophthora cinnamomi causes dieback disease in native vegetation and several crops. The most commonly used chemical to control P. cinnamomi is the oomyceticide phosphite. Despite its widespread use, the mode of action of phosphite is not well understood and it is unclear whether it targets the pathogen, the host, or both. Resistance to phosphite is emergin...
Source: Journal of Proteomics - April 26, 2024 Category: Biochemistry Authors: Christina E Andronis Silke Jacques Francisco J Lopez-Ruiz Richard Lipscombe Kar-Chun Tan Source Type: research
Proteomic analysis revealed that the oomyceticide phosphite exhibits multi-modal action in an oomycete pathosystem
J Proteomics. 2024 Apr 24:105181. doi: 10.1016/j.jprot.2024.105181. Online ahead of print.ABSTRACTPhytopathogenic oomycetes constitute some of the most devastating plant pathogens and cause significant crop and horticultural yield and economic losses. The phytopathogen Phytophthora cinnamomi causes dieback disease in native vegetation and several crops. The most commonly used chemical to control P. cinnamomi is the oomyceticide phosphite. Despite its widespread use, the mode of action of phosphite is not well understood and it is unclear whether it targets the pathogen, the host, or both. Resistance to phosphite is emergin...
Source: Journal of Proteomics - April 26, 2024 Category: Biochemistry Authors: Christina E Andronis Silke Jacques Francisco J Lopez-Ruiz Richard Lipscombe Kar-Chun Tan Source Type: research
Proteomic analysis revealed that the oomyceticide phosphite exhibits multi-modal action in an oomycete pathosystem
J Proteomics. 2024 Apr 24:105181. doi: 10.1016/j.jprot.2024.105181. Online ahead of print.ABSTRACTPhytopathogenic oomycetes constitute some of the most devastating plant pathogens and cause significant crop and horticultural yield and economic losses. The phytopathogen Phytophthora cinnamomi causes dieback disease in native vegetation and several crops. The most commonly used chemical to control P. cinnamomi is the oomyceticide phosphite. Despite its widespread use, the mode of action of phosphite is not well understood and it is unclear whether it targets the pathogen, the host, or both. Resistance to phosphite is emergin...
Source: Journal of Proteomics - April 26, 2024 Category: Biochemistry Authors: Christina E Andronis Silke Jacques Francisco J Lopez-Ruiz Richard Lipscombe Kar-Chun Tan Source Type: research
Proteomic analysis revealed that the oomyceticide phosphite exhibits multi-modal action in an oomycete pathosystem
J Proteomics. 2024 Apr 24:105181. doi: 10.1016/j.jprot.2024.105181. Online ahead of print.ABSTRACTPhytopathogenic oomycetes constitute some of the most devastating plant pathogens and cause significant crop and horticultural yield and economic losses. The phytopathogen Phytophthora cinnamomi causes dieback disease in native vegetation and several crops. The most commonly used chemical to control P. cinnamomi is the oomyceticide phosphite. Despite its widespread use, the mode of action of phosphite is not well understood and it is unclear whether it targets the pathogen, the host, or both. Resistance to phosphite is emergin...
Source: Journal of Proteomics - April 26, 2024 Category: Biochemistry Authors: Christina E Andronis Silke Jacques Francisco J Lopez-Ruiz Richard Lipscombe Kar-Chun Tan Source Type: research
Screening of lung cancer serum biomarkers based on Boruta-shap and RFC-RFECV algorithms
CONCLUSION: This study identified a panel of six serum miRNAs with potential as non-invasive biomarkers for lung cancer diagnosis. These miRNAs show promise in providing sensitive and specific tools for detecting lung cancer.SIGNIFICANCE: Lung cancer is one of the top cancers worldwide, threatening the health and lives of tens of thousands of people. miRNA is a biomarker, which can be used as a potential clinical tool for diagnosis and prognosis of cancer patients. Therefore, the use of multiple miRNAs to construct diagnostic models may be one of the future methods of accurate diagnosis of lung cancer. In this study, we us...
Source: Journal of Proteomics - April 25, 2024 Category: Biochemistry Authors: Guangcheng Yue Source Type: research
Delineating molecular regulatory network of meat quality of longissimus dorsi indicated by transcriptomic, proteomic, and metabolomics analysis in rabbit
This study aims to investigate the potential regulatory network responsible for the meat quality using multi-omics to help developing better varieties. Slaughter performance and meat quality of Shuxing No.1 rabbit outperformed IRA rabbit according to the tested rabbit parameters. Differentially expressed genes (DEGs) and differentially abundance proteins (DAPs) were involved in meat quality-related pathways, such as PI3K - Akt and MAPK signaling pathway. Only SMTNL1 and PM20D2 shared between DEGs and DAPs. Olfactory-sensitive undecanal, a differentially abundant metabolite (DAM) in volatilomics (vDAMs), correlated with all...
Source: Journal of Proteomics - April 24, 2024 Category: Biochemistry Authors: Liangde Kuang Jianhong Zeng Yuying Li Jie Zheng Yongjun Ren Zhiqiang Guo Xiangyu Zhang Cuixia Zhang Chao Yang Xiuli Mei Rui Yang Li Tang Yang Ji Xiaohong Xie Min Lei Congyan Li Source Type: research
Delineating molecular regulatory network of meat quality of longissimus dorsi indicated by transcriptomic, proteomic, and metabolomics analysis in rabbit
This study aims to investigate the potential regulatory network responsible for the meat quality using multi-omics to help developing better varieties. Slaughter performance and meat quality of Shuxing No.1 rabbit outperformed IRA rabbit according to the tested rabbit parameters. Differentially expressed genes (DEGs) and differentially abundance proteins (DAPs) were involved in meat quality-related pathways, such as PI3K - Akt and MAPK signaling pathway. Only SMTNL1 and PM20D2 shared between DEGs and DAPs. Olfactory-sensitive undecanal, a differentially abundant metabolite (DAM) in volatilomics (vDAMs), correlated with all...
Source: Journal of Proteomics - April 24, 2024 Category: Biochemistry Authors: Liangde Kuang Jianhong Zeng Yuying Li Jie Zheng Yongjun Ren Zhiqiang Guo Xiangyu Zhang Cuixia Zhang Chao Yang Xiuli Mei Rui Yang Li Tang Yang Ji Xiaohong Xie Min Lei Congyan Li Source Type: research
Insights into the differential proteome landscape of a newly isolated Paramecium multimicronucleatum in response to cadmium stress
J Proteomics. 2024 Apr 16:105178. doi: 10.1016/j.jprot.2024.105178. Online ahead of print.ABSTRACTEmploying microbial systems for the bioremediation of contaminated waters represent a potential option, however, limited understanding of the underlying mechanisms hampers the implication of microbial-mediated bioremediation. The omics tools offer a promising approach to explore the molecular basis of the bioremediation process. Here, a mass spectrometry-based quantitative proteome profiling approach was conducted to explore the differential protein levels in cadmium-treated Paramecium multimicronucleatum. The Proteome Discove...
Source: Journal of Proteomics - April 18, 2024 Category: Biochemistry Authors: Itrat Zahra Lauren DeVine Robert Cole Hafiza Aroosa Malik Jinke Wu Junneng Wen Amor Hedfi Ayesha Liaqat Roohi Ijaz Uzma Ramzan Abdul Rauf Shakoori Farah Rauf Shakoori Michael J Betenbaugh Source Type: research
Insights into the differential proteome landscape of a newly isolated Paramecium multimicronucleatum in response to cadmium stress
J Proteomics. 2024 Apr 16:105178. doi: 10.1016/j.jprot.2024.105178. Online ahead of print.ABSTRACTEmploying microbial systems for the bioremediation of contaminated waters represent a potential option, however, limited understanding of the underlying mechanisms hampers the implication of microbial-mediated bioremediation. The omics tools offer a promising approach to explore the molecular basis of the bioremediation process. Here, a mass spectrometry-based quantitative proteome profiling approach was conducted to explore the differential protein levels in cadmium-treated Paramecium multimicronucleatum. The Proteome Discove...
Source: Journal of Proteomics - April 18, 2024 Category: Biochemistry Authors: Itrat Zahra Lauren DeVine Robert Cole Hafiza Aroosa Malik Jinke Wu Junneng Wen Amor Hedfi Ayesha Liaqat Roohi Ijaz Uzma Ramzan Abdul Rauf Shakoori Farah Rauf Shakoori Michael J Betenbaugh Source Type: research
Substrate and functional characterization of the lysine acetyltransferase MsKat and deacetylase MsCobB in Mycobacterium smegmatis
In this study, we systematically analyzed the dynamic molecular changes in response to the MsKat/MsCobB overexpression in mycobacteria at proteome and lysine acetylation level by using a TMT-based quantitative proteomic approach. Pathways related with glycolysis, degradation of branched chain amino acids, phosphotransferase system were affected after disturbance of the two regulates enzymes involved in lysine acetylation. We also proved that AMP-dependent synthetase Clp protease, ATP-dependent DNA/RNA helicases and citrate synthase was the substrate of MsKat according to our proteomic data and biological validation. Togeth...
Source: Journal of Proteomics - April 17, 2024 Category: Biochemistry Authors: Yunbo Kan Shuyu Xie Yewen Sun Tong Ye Yunxu Bian Fang Guo Mingya Zhang Tianxian Liu Tianqi Liu Jing Ji Bin Liu Minjia Tan Jun-Yu Xu Source Type: research
Substrate and functional characterization of the lysine acetyltransferase MsKat and deacetylase MsCobB in Mycobacterium smegmatis
In this study, we systematically analyzed the dynamic molecular changes in response to the MsKat/MsCobB overexpression in mycobacteria at proteome and lysine acetylation level by using a TMT-based quantitative proteomic approach. Pathways related with glycolysis, degradation of branched chain amino acids, phosphotransferase system were affected after disturbance of the two regulates enzymes involved in lysine acetylation. We also proved that AMP-dependent synthetase Clp protease, ATP-dependent DNA/RNA helicases and citrate synthase was the substrate of MsKat according to our proteomic data and biological validation. Togeth...
Source: Journal of Proteomics - April 17, 2024 Category: Biochemistry Authors: Yunbo Kan Shuyu Xie Yewen Sun Tong Ye Yunxu Bian Fang Guo Mingya Zhang Tianxian Liu Tianqi Liu Jing Ji Bin Liu Minjia Tan Jun-Yu Xu Source Type: research
