Combinatorial metabolic engineering of Streptomyces sp. CB03234-S for the enhanced production of anthraquinone-fused enediyne tiancimycins
Anthraquinone-fused enediynes (AFEs) are excellent payloads for antibody-drug conjugates (ADCs). The yields of AFEs in the original bacterial hosts are extremely low. Multiple traditional methods had been adop... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 4, 2024 Category: Microbiology Authors: Zhoukang Zhuang, Wenping Kong, Zhongqing Wen, Nian Tong, Jing Lin, Fan Zhang, Zhiying Fan, Liwei Yi, Yong Huang, Yanwen Duan, Xiaohui Yan and Xiangcheng Zhu Tags: Research Source Type: research
The effects of synthesis gas feedstocks and oxygen perturbation on hydrogen production by Parageobacillus thermoglucosidasius
The facultatively anaerobic thermophile Parageobacillus thermoglucosidasius is able to produce hydrogen gas (H2) through the water –gas shift (WGS) reaction. To date this process has been evaluated under controlle... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 2, 2024 Category: Microbiology Authors: Michael Mol, Magda Stephania Ardila, Bronwyn Ashleigh Mol, Habibu Aliyu, Anke Neumann and Pieter de Maayer Tags: Research Source Type: research
Utilizing chitooligosaccharides from shrimp waste biodegradation via recombinant chitinase A: a promising approach for emulsifying hydrocarbon and bioremediation
Hydrocarbon pollution stemming from petrochemical activities is a significant global environmental concern. Bioremediation, employing microbial chitinase-based bioproducts to detoxify or remove contaminants, p... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 2, 2024 Category: Microbiology Authors: Shaimaa A. Nour, Maha T. H. Emam, Ghada M. El-Sayed and Ebtehag A. E. Sakr Tags: Research Source Type: research
High production of ectoine from methane in genetically engineered Methylomicrobium alcaliphilum 20Z by preventing ectoine degradation
Methane is a greenhouse gas with a significant potential to contribute to global warming. The biological conversion of methane to ectoine using methanotrophs represents an environmentally and economically bene... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 2, 2024 Category: Microbiology Authors: Sang Eun Lim, Sukhyeong Cho, Yejin Choi, Jeong-Geol Na and Jinwon Lee Tags: Research Source Type: research
Developing a platform for secretion of biomolecules in Mycoplasma feriruminatoris
Having a simple and fast dividing organism capable of producing and exposing at its surface or secreting functional complex biomolecules with disulphide bridges is of great interest. The mycoplasma bacterial g... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 30, 2024 Category: Microbiology Authors: Javier Gonzalez-de-Miguel, Ariadna Montero-Blay, Ludovica Ciampi, Irene Rodriguez-Arce and Luis Serrano Tags: Research Source Type: research
Carbon dioxide valorization into resveratrol via lithoautotrophic fermentation using engineered Cupriavidus necator H16
Industrial biomanufacturing of value-added products using CO2 as a carbon source is considered more sustainable, cost-effective and resource-efficient than using common carbohydrate feedstocks. Cupriavidus necato... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 27, 2024 Category: Microbiology Authors: Yongjae Jang, Yeon Ji Lee, Gyeongtaek Gong, Sun-Mi Lee, Youngsoon Um, Kyoung Heon Kim and Ja Kyong Ko Tags: Research Source Type: research
Methionine inducing carbohydrate esterase secretion of Trichoderma harzianum enhances the accessibility of substrate glycosidic bonds
The conversion of plant biomass into biochemicals is a promising way to alleviate energy shortage, which depends on efficient microbial saccharification and cellular metabolism. Trichoderma spp. have plentiful CA... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 26, 2024 Category: Microbiology Authors: Yang Liu, Tuo Li, Han Zhu, Linhua Cao, Lebin Liang, Dongyang Liu and Qirong Shen Tags: Research Source Type: research
Investigating human-derived lactic acid bacteria for alcohol resistance
Excessive alcohol consumption has been consistently linked to serious adverse health effects, particularly affecting the liver. One natural defense against the detrimental impacts of alcohol is provided by alc... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 24, 2024 Category: Microbiology Authors: Sini Kang, Jing Long, Myeong Soo Park, Geun Eog Ji, Ying Ju and Seockmo Ku Tags: Research Source Type: research
Multiplex genetic manipulations in Clostridium butyricum and Clostridium sporogenes to secrete recombinant antigen proteins for oral-spore vaccination
Clostridium spp. has demonstrated therapeutic potential in cancer treatment through intravenous or intratumoral administration. This approach has expanded to include non-pathogenic clostridia for the treatment of... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 24, 2024 Category: Microbiology Authors: Yanchao Zhang, Tom S. Bailey, Philip Hittmeyer, Ludwig J. Dubois, Jan Theys and Philippe Lambin Tags: Research Source Type: research
Characterization and optimization of exopolysaccharide extracted from a newly isolated halotolerant cyanobacterium, Acaryochloris Al-Azhar MNE ON864448.1 with antiviral activity
This study aimed to identify and optimize the conditions for exopolysaccharide (EPS) production from a newly i... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 22, 2024 Category: Microbiology Authors: Mabroka H. Saad, Nagwa M. Sidkey and Esmail M. El-Fakharany Tags: Research Source Type: research
Combinatorial metabolic engineering of Bacillus subtilis enables the efficient biosynthesis of isoquercitrin from quercetin
Isoquercitrin (quercetin-3-O- β-D-glucopyranoside) has exhibited promising therapeutic potentials as cardioprotective, anti-diabetic, anti-cancer, and anti-viral agents. However, its structural complexity and l... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 20, 2024 Category: Microbiology Authors: Tengfei Niu, Chaokang Huang, Rufeng Wang, Li Yang, Shujuan Zhao and Zhengtao Wang Tags: Research Source Type: research
PySupercharge: a python algorithm for enabling ABC transporter bacterial secretion of all proteins through amino acid mutation
The process of producing proteins in bacterial systems and secreting them through ATP-binding cassette (ABC) transporters is an area that has been actively researched and used due to its high protein productio... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 20, 2024 Category: Microbiology Authors: Yerin Kim, Danny Kim, Nguyen-Mihn Hieu, Hyunjong Byun and Jung Hoon Ahn Tags: Methodology Source Type: research
Scalable protein production by Komagataella phaffii enabled by ARS plasmids and carbon source-based selection
Most recombinant Komagataella phaffii (Pichia pastoris) strains for protein production are generated by genomic integration of expression cassettes. The clonal variability in gene copy numbers, integration loci a... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 20, 2024 Category: Microbiology Authors: Florian Weiss, Guillermo Requena-Moreno, Carsten Pichler, Francisco Valero, Anton Glieder and Xavier Garcia-Ortega Tags: Research Source Type: research
Engineering Streptomyces sp. CPCC 204095 for the targeted high-level production of isatropolone A by elucidating its pathway-specific regulatory mechanism
Isatropolone A and C, produced by Streptomyces sp. CPCC 204095, belong to an unusual class of non-benzenoid aromatic compounds and contain a rare seven-membered ring structure. Isatropolone A exhibits potent acti... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 16, 2024 Category: Microbiology Authors: Cong Zhang, Qianqian Xu, Jie Fu, Linzhuan Wu, Yihong Li, Yuan Lu, Yuanyuan Shi, Hongmin Sun, Xingxing Li, Lifei Wang and Bin Hong Tags: Research Source Type: research
Optimizing microbioreactor cultivation strategies for Trichoderma reesei: from batch to fed-batch operations
Filamentous fungi have long been recognized for their exceptional enzyme production capabilities. Among these, Trichoderma reesei has emerged as a key producer of various industrially relevant enzymes and is part... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - April 15, 2024 Category: Microbiology Authors: Katja Rohr, Lisa Gremm, Bertram Geinitz, Etienne Jourdier, Wolfgang Wiechert, Fadhel Ben Chaabane and Marco Oldiges Tags: Research Source Type: research
