Machine learning in time-lapse imaging to differentiate embryos from young vs old mice
This study employs time-lapse microscopy and machine learning to elucidate changes in embryonic growth kinetics with maternal aging. We analyzed morphokinetic parameters of embryos from young and aged C57BL6/NJ mice via continuous imaging. Our findings show that aged embryos accelerated through cleavage stages (from 5-cells) to morula compared to younger counterparts, with no significant differences observed in later stages of blastulation. Unsupervised machine learning identified two distinct clusters comprising of embryos from aged or young donors. Moreover, in supervised learning, the XGBoost (extreme gradient boosting)...
Source: Reproductive Biology - April 30, 2024 Category: Reproduction Medicine Authors: Liubin Yang Carolina Leynes Ashley Pawelka Isabel Lorenzo Andrew Chou Brendan Lee Jason D Heaney Source Type: research
The long non-coding RPPH1 is decreased in leukocytes and increased in plasma from women developing pre-eclampsia
In conclusion, RPPH1 expression in leukocytes was significantly decreased in women with pre-eclampsia, and the expression at 22-24 weeks associated with the subsequent development of pre-eclampsia. RPPH1 in leukocytes may be a useful biomarker for prediction and/or early detection of pre-eclampsia and an unknown regulator of the signaling affecting immune cells.PMID:38685609 | DOI:10.1093/biolre/ioae069 (Source: Reproductive Biology)
Source: Reproductive Biology - April 30, 2024 Category: Reproduction Medicine Authors: Dina-Marie Munkelien Myhrer Monica Fr øystad Marie Cecilie Paasche Roland Thor Ueland Tove Lekva Source Type: research
Comparison of assay methods for quantifying sex hormone concentrations across the menstrual cycle in rhesus macaques
Biol Reprod. 2024 Apr 30:ioae063. doi: 10.1093/biolre/ioae063. Online ahead of print.ABSTRACTImmunoassays have been the preferred method for steroid hormone analysis for more than 50 years. Automated immunoassays (AIAs) offer high-throughput, rapid data turnaround, and low cost for measuring steroid hormone concentrations. The application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for steroid quantification provides greater specificity and selectivity for individual steroids, the ability to simultaneously analyze multiple steroids, and high-throughput and automation. We compared AIA and LC-MS/MS for analy...
Source: Reproductive Biology - April 30, 2024 Category: Reproduction Medicine Authors: Kristopher M Fecteau Andrea J Winchell Steven W Blue Maria Luisa Appleman Henryk F Urbanski David W Erikson Source Type: research
Possible Impact of Human β-defensin 1 on sperm motility in infertile men with abnormal sperm parameters
Reprod Biol. 2024 Apr 29;24(2):100887. doi: 10.1016/j.repbio.2024.100887. Online ahead of print.ABSTRACTHuman β-defensins and interleukins may be auxiliary in sperm maturation. This cross-sectional study aimed to evaluate the expression of Human β-defensins 1 and 2, interleukins (ILs)- 10 and -18 genes in sperm, as well as seminal plasma levels of these two cytokines in subfertile men with different types of sperm abnormalities compared to those with normozoospermic men. Participants were separated into two experimental groups: the control group (n = 25) and the group with sperm abnormalities (SA) (n = 45). SA participan...
Source: Reproductive Biology - April 30, 2024 Category: Reproduction Medicine Authors: Amir Masoud Firouzabadi Mohammad Ebrahim Rezvani Fateme Zare Hossein Azizian Farzaneh Fesahat Source Type: research
Should we should consider day 3 blastomere number during single vitrified-warmed blastocyst transfer cycle? A retrospective study
Eur J Obstet Gynecol Reprod Biol. 2024 Apr 18;297:209-213. doi: 10.1016/j.ejogrb.2024.04.021. Online ahead of print.ABSTRACTThe present study investigated whether day 3 blastomere number has an effect on the clinical outcomes during single vitrified-warmed blastocyst transfer cycles. A total of 3294 vitrified-warmed single day 5 blastocyst transferred cycles were analyzed in this retrospective study from January 2018 to December 2021. The cycles were divided into ≥ 7 and < 7 blastomere groups depending on the day 3 embryo blastomere number. The clinical outcomes were compared between the two groups, moreover multivari...
Source: Reproductive Biology - April 30, 2024 Category: Reproduction Medicine Authors: Haisu Zhou Lianmin Ye Huan Zhang Yi Zheng Wumin Jin Source Type: research
Machine learning in time-lapse imaging to differentiate embryos from young vs old mice
This study employs time-lapse microscopy and machine learning to elucidate changes in embryonic growth kinetics with maternal aging. We analyzed morphokinetic parameters of embryos from young and aged C57BL6/NJ mice via continuous imaging. Our findings show that aged embryos accelerated through cleavage stages (from 5-cells) to morula compared to younger counterparts, with no significant differences observed in later stages of blastulation. Unsupervised machine learning identified two distinct clusters comprising of embryos from aged or young donors. Moreover, in supervised learning, the XGBoost (extreme gradient boosting)...
Source: Reproductive Biology - April 30, 2024 Category: Reproduction Medicine Authors: Liubin Yang Carolina Leynes Ashley Pawelka Isabel Lorenzo Andrew Chou Brendan Lee Jason D Heaney Source Type: research
The long non-coding RPPH1 is decreased in leukocytes and increased in plasma from women developing pre-eclampsia
In conclusion, RPPH1 expression in leukocytes was significantly decreased in women with pre-eclampsia, and the expression at 22-24 weeks associated with the subsequent development of pre-eclampsia. RPPH1 in leukocytes may be a useful biomarker for prediction and/or early detection of pre-eclampsia and an unknown regulator of the signaling affecting immune cells.PMID:38685609 | DOI:10.1093/biolre/ioae069 (Source: Reproductive Biology)
Source: Reproductive Biology - April 30, 2024 Category: Reproduction Medicine Authors: Dina-Marie Munkelien Myhrer Monica Fr øystad Marie Cecilie Paasche Roland Thor Ueland Tove Lekva Source Type: research
Comparison of assay methods for quantifying sex hormone concentrations across the menstrual cycle in rhesus macaques
Biol Reprod. 2024 Apr 30:ioae063. doi: 10.1093/biolre/ioae063. Online ahead of print.ABSTRACTImmunoassays have been the preferred method for steroid hormone analysis for more than 50 years. Automated immunoassays (AIAs) offer high-throughput, rapid data turnaround, and low cost for measuring steroid hormone concentrations. The application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) for steroid quantification provides greater specificity and selectivity for individual steroids, the ability to simultaneously analyze multiple steroids, and high-throughput and automation. We compared AIA and LC-MS/MS for analy...
Source: Reproductive Biology - April 30, 2024 Category: Reproduction Medicine Authors: Kristopher M Fecteau Andrea J Winchell Steven W Blue Maria Luisa Appleman Henryk F Urbanski David W Erikson Source Type: research
The interaction of ER stress and autophagy in trophoblasts: navigating pregnancy outcome
Biol Reprod. 2024 Apr 28:ioae066. doi: 10.1093/biolre/ioae066. Online ahead of print.ABSTRACTThe endoplasmic reticulum (ER) is a complex and dynamic organelle that initiates unfolded protein response (UPR) and endoplasmic reticulum stress (ER Stress) in response to the accumulation of unfolded or misfolded proteins within its lumen. Autophagy is a paramount intracellular degradation system that facilitates the transportation of proteins, cytoplasmic components, and organelles to lysosomes for degradation and recycling. Preeclampsia (PE) and intrauterine growth retardation (IUGR) are two common complications of pregnancy as...
Source: Reproductive Biology - April 28, 2024 Category: Reproduction Medicine Authors: Yi Zheng Xia Zha Bei Zhang Mabrouk Elsabagh Hongrong Wang Mengzhi Wang Hao Zhang Source Type: research
Timing of administration of antenatal magnesium sulphate and correlation with umbilical cord blood magnesium levels in preterm babies
CONCLUSION: Mean cord blood magnesium levels served as a tool to determine the timing and duration of Neuroprophylaxis. Mean cord blood magnesium of 3.8 mg/dl should be achieved to serve the purpose of Neuroprotection. To achieve this, Bolus followed by Infusion should be administered for at-least 6 h prior to delivery.PMID:38678795 | DOI:10.1016/j.ejogrb.2024.04.031 (Source: Reproductive Biology)
Source: Reproductive Biology - April 28, 2024 Category: Reproduction Medicine Authors: Mrudhula Tummala Rekha Upadhya Muralidhar V Pai Source Type: research
Meta-analysis of the efficacy of neoadjuvant chemotherapy for locally advanced cervical cancer
CONCLUSIONS: The use of neoadjuvant chemotherapy for the treatment of locally advanced cervical cancer improved the objective response rate and the complete remission rate of patients, but failed to improve overall survival and adverse effects.PMID:38678796 | DOI:10.1016/j.ejogrb.2024.04.023 (Source: Reproductive Biology)
Source: Reproductive Biology - April 28, 2024 Category: Reproduction Medicine Authors: Daying Wang Xiuli Fang Source Type: research
The interaction of ER stress and autophagy in trophoblasts: navigating pregnancy outcome
Biol Reprod. 2024 Apr 28:ioae066. doi: 10.1093/biolre/ioae066. Online ahead of print.ABSTRACTThe endoplasmic reticulum (ER) is a complex and dynamic organelle that initiates unfolded protein response (UPR) and endoplasmic reticulum stress (ER Stress) in response to the accumulation of unfolded or misfolded proteins within its lumen. Autophagy is a paramount intracellular degradation system that facilitates the transportation of proteins, cytoplasmic components, and organelles to lysosomes for degradation and recycling. Preeclampsia (PE) and intrauterine growth retardation (IUGR) are two common complications of pregnancy as...
Source: Reproductive Biology - April 28, 2024 Category: Reproduction Medicine Authors: Yi Zheng Xia Zha Bei Zhang Mabrouk Elsabagh Hongrong Wang Mengzhi Wang Hao Zhang Source Type: research
How does maternal anemia affect the levels of umbilical cord brain-derived neurotrophic factor?
CONCLUSION: In conclusion, the BDNF value of the babies of healthy individuals is higher than that of anemic individuals. Our study showed that the amount of BDNF in the umbilical cord blood was significantly affected by maternal iron deficiency anemia.PMID:38677095 | DOI:10.1016/j.ejogrb.2024.04.016 (Source: Reproductive Biology)
Source: Reproductive Biology - April 27, 2024 Category: Reproduction Medicine Authors: G ökçe İpek Aytok Asuman Akkaya F ırat İlke Mungan Akın Ay şegül Özel Source Type: research
Saliva-based microRNA diagnostic signature for the superficial peritoneal endometriosis phenotype
CONCLUSION: This signature could constitute a new diagnostic strategy to detect the SPE phenotype based on a simple biological test and render diagnostic laparoscopy obsolete. PRéCIS: We generated a saliva-based signature to identify patients with superficial peritoneal endometriosis which is the most challenging form of endometriosis to diagnose and which is often either misdiagnosed or requires invasive laparoscopy.PMID:38677096 | DOI:10.1016/j.ejogrb.2024.04.020 (Source: Reproductive Biology)
Source: Reproductive Biology - April 27, 2024 Category: Reproduction Medicine Authors: Sofiane Bendifallah Yohann Dabi St éphane Suisse Johanna Ilic L éa Delbos Mathieu Poilblanc Philippe Descamps Francois Golfier Ludmila Jornea Delphine Bouteiller Cyril Touboul Anne Puchar Emile Dara ï Source Type: research
DNA damage and ALAD polymorphism in high blood lead (Pb) levels of pregnant women attending a tertiary care teaching hospital
CONCLUSIONS: The combination of comet assay and polymorphism analysis offers a comprehensive approach to understanding the impact of lead exposure during pregnancy. These findings underscore the urgent need for effective regulatory measures to reduce lead exposure in the environment and mitigate its adverse effects of lead on maternal and child health.PMID:38665325 | PMC:PMC11043820 | DOI:10.1016/j.eurox.2024.100300 (Source: Reproductive Biology)
Source: Reproductive Biology - April 26, 2024 Category: Reproduction Medicine Authors: Mahesh Chandra Sahu Kuldip Upadhyay Shweta Gupta Kabita Chanania Sanghamitra Pati Source Type: research
