Minimal criteria for reporting mesenchymal stem cells in veterinary regenerative medicine
AbstractThe widespread application of mesenchymal stem cells (MSCs) in veterinary regenerative medicine highlights their promising therapeutic potential. However, the lack of standardized characterization and reporting practices across studies poses a significant challenge, compromising the assessment of their safety and efficacy. While criteria established for human MSCs serve as a foundation, the unique characteristics of animal-derived MSCs warrant updated guidelines tailored to veterinary medicine. A recent position statement outlining minimal reporting criteria for MSCs in veterinary research reflects efforts to addre...
Source: Veterinary Research Communications - April 27, 2024 Category: Veterinary Research Source Type: research
Empathy and mental health in veterinary medicine
AbstractIn veterinary medicine, the relationship between empathy and mental health presents a complex and important aspect of professional well-being. Veterinarians are frequently exposed to numerous work-related stressors and are therefore more likely to experience mental health disorders and commit suicide. Due to the specific nature of the profession, veterinarians deal with negative patient outcomes, inform owners of unfavourable news, handle heavy workloads, and professional isolation. Psychological stress is a result of all these factors coming together, and it can lead to anxiety, depression, burnout, and even frequ...
Source: Veterinary Research Communications - April 26, 2024 Category: Veterinary Research Source Type: research
Human and camel cystic echinococcosis – a polyclonal antibody-based sandwich ELISA for its serodiagnosis with molecular identification
In conclusion, Sandwich ELISA technique was found to be a potent and sensitive assay for detection of hydatid antigen in both human and camel samples. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - April 26, 2024 Category: Veterinary Research Source Type: research
Changes in biomarkers of the redox status in whole blood and red blood cell lysates in canine hypothyroidism
The objective of this study was to evaluate the changes in the redox status in canine hypothyroidism using whole blood (WB) and red blood cell (RBCs) lysates. For this purpose, a panel of five antioxidants and five oxidants biomarkers was measured in WB and RBCs lysates of 30 dogs with hypothyroidism, 26 dogs with non-thyroidal illnesses and 15 healthy dogs. The antioxidants measured were cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), thiol and paraoxonase type-1 (PON-1). Oxidants measured include the total oxidant status (TOS), peroxi...
Source: Veterinary Research Communications - April 25, 2024 Category: Veterinary Research Source Type: research
Correlation of udder thermogram and somatic cell counts as a tool for detection of subclinical mastitis in buffaloes
This study aimed to monitor the mammary health of 37 multiparous Murrah buffaloes through infrared thermography (IRT). Based on the California Mastitis Test (CMT) and milk somatic cell counts (SCC), buffaloes were grouped into healthy (H,n = 16), subclinical mastitis (SCM,n = 10), and clinical mastitis (CM,n = 11). Buffaloes were milked twice daily in the morning (5:00–6:00 AM) and evening (5:00–6:00 PM). Rectal temperature and respiratory rates were recorded, CMT was performed and thermal images of the mammary gland of all the buffaloes were taken before and after each milking. Milk samples we re analysed ...
Source: Veterinary Research Communications - April 25, 2024 Category: Veterinary Research Source Type: research
Molecular detection of Bartonella spp. in bat-associated macronyssid mites (Acari: Macronyssidae) from Southern and Southeastern Brazil
AbstractDespite the worldwide occurrence and high genetic diversity ofBartonella spp. in bats, few studies investigate their occurrence in bat-associated mites. To date, 26 species of Macronyssidae mite species have been reported from Brazil, and 15 of which were found parasitizing bats. The present study aimed to investigate the presence ofBartonella DNA in bat-associated macronyssid mites from Brazil. For this purpose, 393 macronyssid specimens were selected by convenience from the tissue bank of the Acari Collection of the Instituto Butantan (IBSP). These mites were collected from 14 different bat species in three diffe...
Source: Veterinary Research Communications - April 25, 2024 Category: Veterinary Research Source Type: research
Sex diferences in serum and synovial fluid C-reactive protein concentration in healthy dogs
The objective of this study was to evaluate the disparities in SCRP and SFCRP concentrations between females and males, as well as within each sex. Sixty healthy dogs (N = 60), comprising both sexes, were enrolled in the study. Peripheral blood and knee synovial fluid samples were collected for SCRP and SFCRP analysis, respectively. Serum C-reactive protein (SCRP) and SFCRP concentrations were measured, with mean of 9.61 ± 4.96 mg/L for SCRP and 1.28 ± 3.05 mg/L for SFCRP. Notably, SFCRP concentrations were consistently lower than SCRP concentrations in both sexes. Statistically significant differences wer...
Source: Veterinary Research Communications - April 25, 2024 Category: Veterinary Research Source Type: research
Molecular evidence of sporadic Coxiella burnetii excretion in sheep milk, central Portugal
In this study, a 2-year longitudinal approach was performed to ascertain the excretion ofC. burnetii in bulk tank milk samples of sheep from a mountain plateau in central Portugal, with sampling conducted during the years 2015 and 2016. From a total of 156 bulk tank milk samples tested by qPCR, only one showed to be positive forC. burnetii (1.28% [95%CI: 0.03 –6.94]), from 2015, the first year of collection. Bidirectional sequencing and phylogenetic analysis ofIS1111 transposase partial region confirmed the presence ofC. burnetii DNA. The presence ofC. burnetii in raw milk samples highlights the necessity for additional ...
Source: Veterinary Research Communications - April 24, 2024 Category: Veterinary Research Source Type: research
Description of postoperative complications and bacterial contamination of wound soaker catheters used to administer postoperative local analgesia after mastectomy in 11 dogs: case series
The objectives of this case series were to describe the use of WSC for owner administration of postoperative local analgesia in dogs with mammary tumors treated surgically, to identify complications associated with WSC and to determine the frequency of bacterial colonization of the catheters. Twelve WSC were placed in 11 dogs during mastectomy surgery, left in place for three days, protected by a dressing and successfully managed by owners at home. No postoperative antibiotics were administered. No complications were identified in any cases. No bacterial growth was identified on bacteriological analysis of the twelve WSC. ...
Source: Veterinary Research Communications - April 24, 2024 Category: Veterinary Research Source Type: research
A combination of biomarkers for predicting stallion sperm fertility
AbstractEquine breeding would benefit greatly from reliable biomarkers of stallion or ejaculate fertility. The aim of the study was to investigate how several in vitro sperm characteristics correlate with fertility after artificial insemination, to explore the potential to build a fertility prediction model for stallions. Cooled insemination doses (3 –5 per stallion) were obtained from various studs. Sperm membrane integrity, acrosome integrity, chromatin integrity, mitochondrial membrane potential, and reactive oxygen species production were evaluated by flow cytometry 24–30 h after semen collection, and sperm motili...
Source: Veterinary Research Communications - April 23, 2024 Category: Veterinary Research Source Type: research
Clinico-haematobiochemical and cardiac alterations in Trypanosoma evansi infected buffaloes of Andhra Pradesh, India
AbstractThe present research aimed to document the incidence, clinical signs, haematological, and serum biochemical alterations, as well as electrocardiography and echocardiography findings in 62 buffaloes (selected from a total of 240) infected withTrypanosoma evansi. The study spanned one year, from January 2022 to December 2022. Morphological identification ofTrypanosoma evansi was done by the presence of a centrally positioned nucleus with a small sub-terminal kinetoplast at the posterior position through microscopic examination of Giemsa stained peripheral blood smears. The incidence of trypanosomosis were determined ...
Source: Veterinary Research Communications - April 23, 2024 Category: Veterinary Research Source Type: research
Sheep abortions associated with Neospora caninum and Toxoplasma gondii infections in multiple flocks from Southern Brazil
This study verified the occurrence of these diseases in sheep fetuses from Santa Catarina State, Southern Brazil from 2015 to 2022. Sheep fetuses were necropsied with organ sampling for histopathology, and polymerase chain reaction (PCR) forNeospora caninum andToxoplasma gondii using the Nc5 and SAG2 targets, respectively, in frozen brain tissue. Microbiological culture and RT-PCR forPestivirus were conducted to discard other abortion causes. One positive fetus for toxoplasmosis was genotyped using multiplex multilocus nested PCR-RFLP (Mn-PCR-RFLP) with ten genetic markers. Fifty-five sheep fetuses were evaluated, with 10 ...
Source: Veterinary Research Communications - April 23, 2024 Category: Veterinary Research Source Type: research
Molecular investigation and genetic characterization of feline leukemia virus (FeLV) in cats referred to a veterinary teaching hospital in Northern Italy
In this study, a quantitative SYBR Green Real-Time PCR (qPCR) assay was used to detect FeLV proviral DNA in blood samples from antigen positive cats referred to a veterinary teaching hospital in Northern Italy in 2018 –2021. To genetically characterize the identified viruses, a portion of the viral envelope (env) gene was amplified using six different end-point PCRs and sequenced. Twenty-two of 26 (84.6%) cats included in the study tested positive by qPCR assay. This suggests a high performance of the qPCR adopted but further studies are required to investigate the cause of discordant results between the antigen test and...
Source: Veterinary Research Communications - April 22, 2024 Category: Veterinary Research Source Type: research
Mitigating antimicrobial resistance, an approach to stewardship in canine urinary tract infection
AbstractUrinary tract infection (UTI) caused by antimicrobial resistant bacteria is common in dogs leading to serious health impact in pet animal as well as on human health. Understanding the prevalent uropathogens and their drug susceptibility is essential for limiting the antimicrobial resistance through implementation of stewardship policies. In view of this, present study was envisaged to determine the prevalent bacterial uropathogens and their antibiogram from clinical cases of canine UTI. Urine samples were collected from 35 dogs presented with clinical signs of UTI and a total of 27 bacterial isolates were recovered...
Source: Veterinary Research Communications - April 22, 2024 Category: Veterinary Research Source Type: research
Current status of Nosema spp. infection cases in apis mellifera in eurasian countries and Ptp3 gene haplotypes in the Republic of Tatarstan, Russia
This study expands the data regarding existing haplotypes ofNosema species: there are currently 9 shared and 56 uniquePtp3 nucleotide sequence haplotypes ofN. ceranae, and 2 shared and 7 unique haplotypes ofN. apis, respectively. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - April 22, 2024 Category: Veterinary Research Source Type: research
