Allergy & Immunology News 
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 6.
WAL-DRYL SEVERE ALLERGY AND SINUS HEADACHE (Acetaminophen, Diphenhydramine Hcl, Phenylephrine Hcl) Tablet [WALGREEN CO.]
Updated Date: Dec 6, 2012 EST
Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST) - December 6, 2012 Category: Drugs & Pharmacology Source Type: news
ALL DAY ALLERGY (Cetirizine Hydrochloride) Solution [Amerisource Bergen]
Updated Date: Dec 5, 2012 EST
Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST) - December 5, 2012 Category: Drugs & Pharmacology Source Type: news
CHILDRENS ALLERGY RELIEF (Loratadine) Solution [CVS Pharmacy]
Updated Date: Dec 5, 2012 EST
Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST) - December 5, 2012 Category: Drugs & Pharmacology Source Type: news
CHILDRENS ALL DAY ALLERGY (Cetirizine Hydrochloride) Solution [McKesson]
Updated Date: Dec 5, 2012 EST
Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST) - December 5, 2012 Category: Drugs & Pharmacology Source Type: news
Food Allergies Linked To Pesticides
WebMD Medical News
By Brenda Goodman, MA
Reviewed by Louise Chang, MD
Dec. 3, 2012 — People exposed to higher levels of certain germ- and weed-killing chemicals may also be more likely to develop food allergies, a new study shows.
The chemicals are called dichlorophenols (DCPs). They are created by the breakdown of common pesticides, including chlorinated chemicals used to purify drinking water. They also turn up in moth balls, air fresheners, deodorizer cakes in urinals, and certain herbicides sprayed on crops.
“They’re quite common,” says researcher Elina Jerschow, MD, an allergist at Montefiore ...
Source: WBZ-TV - Breaking News, Weather and Sports for Boston, Worcester and New Hampshire - December 4, 2012 Category: Consumer Health News Authors: mreal197 Tags: WebMD News Source Type: news
Food Allergies Linked to Pesticides
Title: Food Allergies Linked to PesticidesCategory: Health NewsCreated: 12/4/2012 11:00:00 AMLast Editorial Review: 12/4/2012 12:00:00 AM
Source: MedicineNet Allergies General - December 4, 2012 Category: Allergy & Immunology Source Type: news
Tap-Water Chemical May Be Linked to Food Allergy
Pesticide-treated fruits, vegetables may also harbor the compound, researchers say
Source: HealthDay
Related MedlinePlus Pages: Drinking Water, Food Allergy, Pesticides
Source: MedlinePlus Health News - December 3, 2012 Category: Consumer Health News Source Type: news
Assembly of Matched Alpha/Beta Subunits to HLA Class II Peptide Receptors
Human antigen presenting cells express three human leukocyte antigen (HLA) class II isotypes (DR, DP, and DQ), which are composed of polymorphic α and β subunits. The combination of polymorphic α- and β-chains results in cis (encoded on the same chromosome) or trans (encoded on different chromosomes) combinations. Since some of the α–β combinations may yield mismatched non-functional α–β heterodimers, it is not entirely clear which type of HLA class II peptide receptors are found on the cell surface of antigen presenting cells. We have developed a combination of bioch...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Pulse–Chase Analysis for Studies of MHC Class II Biosynthesis, Maturation, and Peptide Loading
Pulse–chase analysis is a commonly used technique for studying the synthesis, processing and transport of proteins. Cultured cells expressing proteins of interest are allowed to take up radioactively labeled amino acids for a brief interval (“pulse”), during which all newly synthesized proteins incorporate the label. The cells are then returned to nonradioactive culture medium for various times (“chase”), during which proteins may undergo conformational changes, trafficking, or degradation. Proteins of interest are isolated (usually by immunoprecipitation) and resolved by sodium dodecyl sulfat...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Gene Transduction in Human Monocyte-Derived Dendritic Cells Using Lentiviral Vectors
Monocyte-derived dendritic cells (MDDCs) are widely used in the field of human immunology. Although a variety of gene delivery procedures have been used in MDDC, it has remained difficult to achieve robust gene transductions. In this chapter, we describe a procedure for high efficiency gene transduction in human MDDCs using lentiviral vectors. Gene transduction based on HIV-1-derived lentiviral vectors is restricted at the level of reverse transcription by the cellular protein SAMHD1 in MDDCs. Co-transduction of the MDDCs with helper particles derived from SIVmac that contain the viral protein Vpx removes this restriction,...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Analysis of Proteasome Generated Antigenic Peptides by Mass Spectrometry
Mass spectrometry (MS) is today one of the most important analytical techniques in biosciences. The development of electro spray ionization (ESI) as a gentle ionization method, in which molecules are not destroyed, has revolutionized the analytic of peptides. MS is an ideal technique for detection and analysis of peptides generated by in vitro experiments using purified 20S proteasomes. It also provides a convenient and sensitive way to monitor the processing activity of enzymes. The combination of high performance liquid chromatography (HPLC) with ESI-MS allows the analysis of complex samples with separation in their spec...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Preparing Antigens Suitable for Cross-presentation Assays In Vitro and In Vivo
Cross-presentation is defined as the ability of certain professional antigen-presenting cells to take up, process and present extracellular antigens on major histocompatibility class I (MHC-I) molecules to CD8+ T cells. The stimulation of naive cytotoxic CD8+ T cells by this process, termed cross-priming, is involved in many different responses, including those to tumors, pathogens, graft tissues, and self-antigens. Dendritic cells (DCs), a heterogeneous cell population, are endowed with the highest cross-priming capacity. Investigation of their cross-presentation capacities, important both for vaccination and for the indu...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Isolation of a Specialized, Antigen-Loaded Early Endosomal Subpopulation by Flow Cytometry
Isolation and characterization of antigen-containing endosomes remains difficult utilizing standard purification techniques. Here, we describe a method, which allows isolation of antigen-loaded endosomes, that is based on flow cytometrical analysis and sorting. We specifically isolated antigen-containing endosomes from cells that had taken up fluorochrome-labeled ovalbumin via mannose receptor-mediated endocytosis. The protocol described here allows for the isolation of pure fractions of ovalbumin-containing endosomes and the extraction of proteins from these endosomes for analysis by western blot. Importantly, this protoc...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Monitoring the Intracellular Routing of Internalized Antigens by Immunofluorescence Microscopy
Professional antigen-presenting cells such as dendritic cells (DCs) and macrophages internalize extracellular antigens, process them intracellularly, and present the resulting antigen-derived peptides in the context of MHC I or MHC II molecules. Since the intracellular routing of the antigen determines whether antigens are presented on MHC I or MHC II molecules, a profound analysis of the intracellular distribution of the internalized antigens is of high interest. Here, we describe an immunofluorescence protocol to monitor the intracellular routing of the model-antigen Ovalbumin in bone marrow-derived dendritic cells (BM-D...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Monitoring Dendritic Cell Activation and Maturation
Since the 1997 discovery that the first identified human homologue of Drosophila Toll could activate the innate immune system, the innate arm of immunity has rapidly taken on a new light as an important player in the recognition of pathogens and damaged self. The recognition of danger by dendritic cells (DC) is a crucial step in activating the adaptive immune system. Different DC express varied subsets of pattern recognition receptors (PRR), enabling both overlap and exclusivity in the recognition of danger signals by DC. PRR-mediated DC maturation and activation can be measured by changes in the surface expression of cost...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Preparation of Dendritic Cells by In Vitro Cultures
In vitro cultures of bone marrow-derived precursors are a convenient method for generating dendritic cells (DC). This method additionally overcomes the problem of low availability of certain DC types, DC heterogeneity, and laborious procedures encountered using ex vivo isolation protocols.
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
The Purification of Large Numbers of Antigen Presenting Dendritic Cells from Mouse Spleen
Dendritic cells (DC) are found at low frequency in lymphoid and non-lymphoid tissues. Different DC subsets are adept at different roles in immunity in diverse scenarios of attack by infectious agents, as well as in the maintenance of self-tolerance. A key element in the ability of DC to initiate adaptive immune responses is their capacity to capture and process antigen, whether from pathogens, vaccines or self-components, and present it to T cells. Our typical procedure for isolation of the different DC types from murine spleen involves their digestion from the tissue using collagenase, selection of cells of light density,...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Tracking Antigen-Specific CD8+ T Cells Using MHC Class I Multimers
The tracking of epitope-specific T cells is a useful approach for the study of adaptive immune responses. This protocol describes how Major Histocompatibility Complex Class I (MHC-I) multimers can be used to stain, enrich, and enumerate (rare) populations of CD8+ T cells specific for a given antigen. It provides the detailed steps for multimer labeling, magnetic enrichment, and cytometric analysis. Additionally, it provides informations for multiplexing experiments in order to achieve simultaneous detection of multiple antigenic specificities, and strategies for coupling the protocol with functional assays (e.g., intracell...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Production of CD4+ and CD8+ T Cell Hybridomas
T cell hybridomas are very useful tools to investigate antigen presenting cell (APC) function. They were developed based on the fusion technology that led to monoclonal antibody section. Antigen-specific primary T cells are generated and fused to an immortal thymoma line. Unfused thymoma cells are eliminated by engineered metabolic selection. Antigen-specific hybridomas are identified and may be characterized in detail. Primary T cells are preferable for studies of the regulatory mechanisms intrinsic to T cells, but for study of antigen presentation T cell hybridomas have advantages over primary T cell clones, including th...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Cloning CD8+ Cytolytic T Lymphocytes
CD8+ T lymphocyte cloning has resulted in many fundamental advances: structural elucidation of peptide-MHC recognition, spatiotemporal dissection of the thymic positive and negative selection processes and is further expected, TCRs being the key molecules controlling T cell activation, to provide us with molecular tools of immuno-therapeutical interest for infectious, tumor, and autoimmune diseases. However, cloning CD8+ T lymphocytes remains a relatively difficult enterprise. Cloning mouse CD8+ T lymphocytes that will be first consider is to some extend facilitated by our complete control of the in vivo priming process an...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Evaluating CD8+ T Cell Responses In Vitro
The 51Cr-release assay described in the 1960s has been for decades the gold standard cytolytic assay and remains in use in many laboratories. Whereas other radioactive tests were later on described, they never fully replaced the 51Cr-release assay. More thorough understanding of CTL biology and killing pathways has more recently resulted in the design of reliable nonradioactive tests to analyze CD8+ T cell responses which are likely to supplant in a close future the 51Cr-release assay.
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Purification of Large Cytosolic Proteases for In Vitro Assays: 2S and 26S Proteasomes
Proteasomes are the main cytosolic proteases responsible for generating peptides for antigen processing and presentation in the MHC (major histocompatibility complex) class-I pathway. Purified 20S and 26S proteasomes have been widely used to study both specificity and efficiency of antigen processing. Here, we describe the purification of active human 20S and 26S proteasomes from human erythrocytes by DEAE-ion exchange chromatography, ammonium sulfate precipitation, glycerol density gradient centrifugation, and Superose-6 size exclusion chromatography and their characterization using fluorogenic substrates and specific inhibitors.
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Bioinformatics Identification of Antigenic Peptide: Predicting the Specificity of Major MHC Class I and II Pathway Players
Bioinformatics methods for immunology have become increasingly used over the last decade and now form an integrated part of most epitope discovery projects. This wide usage has led to the confusion of defining which of the many methods to use for what problems. In this chapter, an overview is given focusing on the suite of tools developed at the Technical University of Denmark.
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Recombinant Poxviruses: Versatile Tools for Immunological Assays
The study of antigen processing and presentation is critical to our understanding of the mechanisms that govern immune surveillance. A typical requirement of assays designed to examine antigen processing and presentation is the de novo biosynthesis of a model antigen. Historically, Vaccinia virus (VACV), a poxvirus closely related to Cowpox, has enjoyed widespread use for this purpose. Recombinant poxvirus-based expression has a number of advantages over other systems. Poxviruses accommodate the insertion of large pieces of recombinant DNA into their genome, and recombination and selection are relatively efficient. Poxviru...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Purification, Preparation, and Use of Chaperone–Peptide Complexes for Tumor Immunotherapy
We describe a method for purifying Hsp70–peptide complexes that can be used to prepare molecular chaperone-based vaccines, involving sequential gel filtration, ion exchange, and affinity chromatography
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Identifying Source Proteins for MHC Class I-Presented Peptides
Identification of antigenic peptides recognized by cytolytic T lymphocytes (CTL) is a prerequisite for the development of targeted cancer immunotherapy approaches. This chapter provides a global approach for the identification of peptides recognized by CTL. It implies the identification of the HLA molecule presenting the peptide as well as the design and screening of a cDNA library derived from the tumor cells.
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Biochemical Analysis of Naturally Processed Antigenic Peptides Presented by MHC Class I Molecules
Immune surveillance of infected or tumor cells by CD8+ T cells requires that MHC class I molecules present a diverse repertoire of peptides on the cell surface. Even a few copies of individual peptides among this mixture are sufficient for recognition by the antigen receptors of appropriate CD8+ T cells. Here we describe a method for biochemical analysis of the naturally processed peptides in living cells. The peptide mixture in cell extracts is fractionated using reverse phase high performance liquid chromatography and detected by the activation of CD8+ T cell hybridomas. The results provide information on the structure a...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Quantitating MHC Class I Ligand Production and Presentation Using TCR-Like Antibodies
Accurately determining the number of peptide–MHC class I complexes on the cell surface is necessary when evaluating cellular processes or pharmaceuticals that alter the antigen presentation machinery. Here I describe a quantitative flow cytometry application for determining the number of peptide–MHC complexes on the surface of cells grown in tissue culture that express an endogenous protein from which the peptide is derived. The procedure requires a monoclonal antibody with the ability to distinguish MHC class I molecules presenting the peptide of interest from other peptide–MHC complexes. Fluorescence si...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Establishing MHC Class I Peptide Motifs
Major histocompatibility complex (MHC) class I peptide motifs are used on a regular basis to identify and predict MHC class I ligands and CD8+ T-cell epitopes. This approach is above all an invaluable tool for the identification of disease-associated epitopes. As a matter of fact, the vast majority of T-cell epitopes discovered during the past two decades was identified by means of epitope prediction. Here we describe the steps which are necessary to establish MHC class I peptide motifs and to compose a reliable scoring matrix for epitope prediction. As an example, a scoring matrix for the prediction of HLA-B*35-presented ...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Biochemical Large-Scale Identification of MHC Class I Ligands
The large-scale identification of MHC class I presented peptides is indispensable for gaining insight into the fundamental rules of immune recognition as well as it is an invaluable tool in identifying potential targets for the immunotherapy of disease. In this chapter we briefly review the existing strategies for the analysis of MHC ligandomes and provide an in-depth protocol for the immunoaffinity purification of MHC class I presented peptides from primary tissues or cells.
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Determining the Activity of the Transporter Associated with Antigen Processing in the Compartments of the Secretory Pathway
Peptide-receptive MHC class I molecules and the TAP (transporter associated with antigen processing) peptide transporter are known to leave the ER and cycle through the cis side of the Golgi apparatus. The amount, and the extent of the activity, of TAP in post-ER compartments is likely to vary between different cell types. Here we describe a convenient microscopic assay to determine it.
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
Evaluation of Viral Interference with MHC Class I-Restricted Antigen Processing and Presentation Using a Flow Cytometry-Based Approach
The peptide content of MHC class I molecules present at the cell surface is monitored by surveilling CD8+ cytotoxic T cells. In case of a viral infection, a proportion of the MHC class I molecules will carry peptides derived from viral proteins. This allows the CD8+ T cells to recognize and eliminate virus-infected cells. This highly sensitive detection system of the host is counteracted by viruses, which have acquired functions to downregulate cell surface expression of MHC class I molecules. In this chapter, we describe a flow cytometry-based method to identify viral gene product(s) responsible for evasion from MHC class...
Source: Springer protocols feed by Immunology - December 3, 2012 Category: Allergy & Immunology Source Type: news
What is the Westley Croup Score?
Discussion
Croup or acute tracheolaryngobronchitis is a common, self-limited disease caused by common respiratory pathogens especially Human Parainfluenza virus.
It can occur year round but often comes in waves particularly in fall, winter and early spring. Three percent of 6
Questions for Further Discussion
1. What are your indications for croup treatment with dexamethasone?
2. What causes respiratory distress?
Related Cases
Disease: Croup | Throat Disorders
Symptom/Presentation: Respiratory Distress
Specialty: Allergy / Pulmonary Diseases | General Pediatrics
Age: Preschooler
To Learn More
To view pediatric review ...
Source: PediatricEducation.org - December 2, 2012 Category: Pediatrics Authors: Donna M. D'Alessandro, M.D. Tags: Uncategorized Source Type: news
Sneezin’s’ greetings: How to avoid holiday allergy triggers
By Andrew MacGinnitie, MD, PhD, associate clinical director of the Division of Immunology at Children’s Hospital Boston
The holidays are a lot of fun for children and adults alike, but for those with allergies and asthma the season can be a little difficult at times. Homemade treats, seasonal decorations and visiting friends and family can all be potential allergy and/or asthma triggers. Here are a few easy ways to avoid some of the more common offenders this winter.
Holiday food
Holiday celebrations are often filled with new and different foods. From plates of cookies to potlucks where everyone brings their favorite dis...
Source: Thrive, Children's Hospital Boston - November 30, 2012 Category: Pediatrics Authors: Guest Blogger Tags: All posts Asthma & allergies Food allergies Milk allergies holidays seasonal allergies Source Type: news
Asthma and nasal congestion common insomnia triggers
Insomnia is a common problem in patients with asthma, suggests research published in Allergy.
Source: MedWire News - Respiratory - November 29, 2012 Category: Respiratory Medicine Source Type: news
Eczema, Egg Allergy in Infants May Predict Peanut AllergyEczema, Egg Allergy in Infants May Predict Peanut Allergy
Infants diagnosed with severe eczema or an egg allergy may be more likely to develop peanut allergy, according to early results from the Learning Early About Peanut Allergy study. Medscape Medical News
Source: Medscape Today Headlines - November 28, 2012 Category: Consumer Health News Tags: Allergy & Clinical Immunology News Source Type: news
Sukhi's Gourmet Indian Foods issues voluntary recall of Sukhi's Red Curry Vegetables due to undeclared Shrimp
Sukhi’s Gourmet Indian Foods is alerting customers that because of a label error, a single lot code of Red Curry with Vegetables contains undeclared shrimp. Consumers who have an allergy or severe sensitivity to shrimp, run the risk of serious or life-threatening allergic reactions if they consume this product.
Source: Food and Drug Administration - November 28, 2012 Category: Food Science Source Type: news
Serological findings in patients with allergic bronchopulmonary aspergillosis during remission
Bhatnagar PK, Banerjee B, Sarma PU
Source: The Aspergillus Website - articles - November 28, 2012 Category: Respiratory Medicine Source Type: news
The allergenic and antigenic properties of spore extracts of Aspergillus fumigatus: a comparative study of spore extracts with mycelium and culture filtrate extracts
Kauffman HF, van der Heide S, Beaumont F, de Monchy JG, de Vries K
Source: The Aspergillus Website - articles - November 28, 2012 Category: Respiratory Medicine Source Type: news
ALLERGY RELIEF (Fexofenadine Hydrochloride) Tablet, Film Coated [H E B]
Updated Date: Nov 28, 2012 EST
Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST) - November 28, 2012 Category: Drugs & Pharmacology Source Type: news
CHILDRENS ALLERGY RELIEF (Cetirizine Hydrochloride) Solution [Wal-Mart Stores Inc]
Updated Date: Nov 28, 2012 EST
Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST) - November 28, 2012 Category: Drugs & Pharmacology Source Type: news
Selecta partners with Sanofi to develop anti-allergy immunotherapies
Clinical-stage biopharmaceutical company Selecta Biosciences has signed a strategic global collaboration agreement with Sanofi to develop up to three antigen-specific immunotherapies for life-threatening allergies.
Source: Pharmaceutical Technology - November 27, 2012 Category: Pharmaceuticals Source Type: news
[Editors' Choice] Ceramide Keeps Mast Cells in Check
A receptor that binds to extracellular ceramide inhibits mast cell activation and allergic responses in vivo.
Source: Signal Transduction Knowledge Environment - November 27, 2012 Category: Science Authors: Nancy Gough (mailto:ngough at aaas.org) Source Type: news
ALLERGENIC MICROORGANISMS AND HYPERSENSITIVITY
James H. Day and Anne K. Ellis
Source: The Aspergillus Website - articles - November 27, 2012 Category: Respiratory Medicine Source Type: news
Fungi and allergic lower respiratory tract diseases
Knutsen AP, Bush RK, Demain JG, Denning DW, Dixit A, Fairs A, Greenberger PA, Kariuki B, Kita H, Kurup VP, Moss RB, Niven RM, Pashley CH, Slavin RG, Vijay HM, Wardlaw AJ
Source: The Aspergillus Website - articles - November 27, 2012 Category: Respiratory Medicine Source Type: news
Food Allergies in Kids: Putting the Guideline Into PracticeFood Allergies in Kids: Putting the Guideline Into Practice
AAP and AAAAI have provided several resources for clinicians to help in managing these hard-to-diagnose and hard-to-manage conditions in kids. Medscape Pediatrics
Source: Medscape Today Headlines - November 27, 2012 Category: Consumer Health News Tags: Allergy & Clinical Immunology Expert Interview Source Type: news
NHS Immunisation Statistics - England, 2011-12
Summary The information in this bulletin, about immunisation statistics in England, comes from: The Health Protection Agency (HPA) and Health Protecti...
Source: The IC : Immunisation - November 27, 2012 Category: Allergy & Immunology Source Type: news
