Biochemistry
This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. 
Subscribe to this data using MyMedWorm.
Subscribe to this data using GoogleReader.
Subscribe to this data using Bloglines.
Subscribe to this data using MyYahoo.
Get the very latest Swine Flu news via the MedWorm Swine Flu RSS news feed - updated hourly from thousands of authoritative health and news sources.
This page shows you the most recent publications within this specialty of the MedWorm directory.
Contrasting Synergistic Anion Effects in Vanadium(V) Binding to Nicatransferrin versus Human Serum Transferrin
Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 20, 2009 Category: Biochemistry Tags: article Source Type: journals
Phosphorylation of Serine 205 by the Protein Kinase CK2 Persists on Pax3-FOXO1, but Not Pax3, throughout Early Myogenic DifferentiationEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 20, 2009 Category: Biochemistry Tags: article Source Type: journals
The Red Blood Cell Proteome and Interactome: An UpdateEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Journal of Proteome Research, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Journal of Proteome Research)
Source: Journal of Proteome Research - November 20, 2009 Category: Biochemistry Tags: article Source Type: journals
Metabonomics in Ulcerative Colitis: Diagnostics, Biomarker Identification, And Insight into the PathophysiologyEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Journal of Proteome Research, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Journal of Proteome Research)
Source: Journal of Proteome Research - November 20, 2009 Category: Biochemistry Tags: article Source Type: journals
Ceramide Kinase Profiling by Mass Spectrometry Reveals a Conserved Phosphorylation Pattern Downstream of the Catalytic SiteEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Journal of Proteome Research, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Journal of Proteome Research)
Source: Journal of Proteome Research - November 20, 2009 Category: Biochemistry Tags: article Source Type: journals
Enhancing Nitrite Reductase Activity of Modified Hemoglobin: Bis-tetramers and Their PEGylated DerivativesEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 20, 2009 Category: Biochemistry Tags: article Source Type: journals
According To The Statistical Analysis Of Fossils 'Hobbits' Are A New Human SpeciesEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Researchers from Stony Brook University Medical Center in New York have confirmed that Homo floresiensis is a genuine ancient human species and not a descendant of healthy humans dwarfed by disease. Using statistical analysis on skeletal remains of a well-preserved female specimen, researchers determined the "hobbit" to be a distinct species and not a genetically flawed version of modern humans. (Source: Biology / Biochemistry News From Medical News Today)
Source: Biology / Biochemistry News From Medical News Today - November 20, 2009 Category: Biochemistry Tags: Biology / Biochemistry Source Type: news
Bioactive caffeic acid esters from Glycyrrhiza glabra.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Thin layer chromatography bioautography (using DPPH spray reagent) guided fractionation of Glycyrrhiza glabra led to the isolation of two caffeic acid derivative esters, viz. eicosanyl caffeate (1) and docosyl caffeate (2). The two compounds exhibited potent elastase inhibitory activity, with IC(50) values of 0.99 microg mL(-1) and 1.4 microg mL(-1) for 1 and 2, respectively. The compounds also showed moderate antioxidant activity in DPPH and ABTS scavenging assays. The results indicate a possible role of caffeic acid derivatives, in addition to flavonoids in the anti-ulcer properties of G. glabra.
PMID: 19921583 [...
Source: Natural Product Research - November 20, 2009 Category: Biochemistry Authors: Dey S, Deepak M, Setty M, D'Souza P, Agarwal A, Sangli GK Tags: Nat Prod Res Source Type: journals
A new xenicane norditerpene from the Indian marine gorgonian Acanthogorgia turgida.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Chemical investigation of the liposoluble extract of the gorgonian Acanthogorgia turgida, from Indian coasts, led us to isolate a new xenicane-based norditerpene, isoacalycixeniolide-A (1), along with the known structurally related compounds 2-6. The structure of the norditerpene (1) was elucidated by spectral methods (mainly by NMR techniques), whereas the absolute stereochemistry was suggested by the application of circular dicroism methodology.
PMID: 19921584 [PubMed - as supplied by publisher] (Source: Natural Product Research)
Source: Natural Product Research - November 20, 2009 Category: Biochemistry Authors: Manzo E, Ciavatta ML, Gavagnin M, Villani G, Quaranta C, D'Souza L, Cimino G Tags: Nat Prod Res Source Type: journals
Clausmarin-C: a novel terpenoid coumarin from Clausena pentaphylla.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
A novel terpenoid coumarin, clausmarin-C (1), has been isolated from Clausena pentaphylla. Its structure has been established by extensive 1D- and 2D-NMR analysis and chemical transformation.
PMID: 19921585 [PubMed - as supplied by publisher] (Source: Natural Product Research)
Source: Natural Product Research - November 20, 2009 Category: Biochemistry Authors: Raj K, Sharma M, Misra N Tags: Nat Prod Res Source Type: journals
Studies on the anti-oedematogenic properties of a fraction rich in lonchocarpin and derricin isolated from Lonchocarpus sericeus.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The acute anti-inflammatory properties of a fraction rich in the chalcones lonchocarpin and derricin, from the roots of Lonchocarpus sericeus (HFLS), were studied for the first time, using a paw oedema model induced in rats by various stimuli. Results showed that HFLS (100 and 200 mg kg(-1), i.p.) was ineffective in inhibiting dextran-induced paw oedema. The HFLS (200 mg kg(-1), p.o. or i.p.) also failed to inhibit the bradykinin-induced oedema. In the yeast-elicited oedema, the HFLS (200 mg kg(-1), i.p.) caused inhibitions ranging from 42 to 59% in the first to fourth hours. Orally administered HFLS (200 mg kg(-1)) wa...
Source: Natural Product Research - November 20, 2009 Category: Biochemistry Authors: Fontenele JB, Leal LK, Felix FH, Silveira ER, Viana GS Tags: Nat Prod Res Source Type: journals
Determination of flavonoid compounds from Saussurea involucrata by liquid chromatography electrospray ionisation mass spectrometry.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Saussurea involucrata produces several bioactive flavonoids that are derived from the phenylpropanoid pathway. To determine these flavonoids, a sensitive high performance liquid chromatography electrospray ionisation mass spectrometry method (LC-ESI-MS) was developed. Chromatographic separation was then performed. The gradient elution was optimised to give high recoveries and satisfactory chromatographic resolution. Flavonoid detection was carried out using an ion trap as mass analyser. Parameters of the mass analyser were optimised. We used the validated LC-ESI-MS method to verify the identities of bioactive compounds...
Source: Natural Product Research - November 20, 2009 Category: Biochemistry Authors: Xu YJ, Zhao DX, Fu CX, Cheng LQ, Wang NF, Han LJ, Ma FS Tags: Nat Prod Res Source Type: journals
The effect of triptolide on CD4+ and CD8+ cells in the Peyer's patch of DA rats with collagen induced arthritis.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this study, we investigated the effect of triptolide on the CD4+ and CD8+ cell distribution in Peyer's patch cells and periphery lymphocytes and TGF-beta and IFN-gamma levels in periphery in collagen induced arthritis (CIA) in DA rats. CIA in the rat is a widely studied animal model of inflammatory polyarthritis, with similarities to RA, and the DA rat is one of most susceptible strains for CIA. Our data show that triptolide could lower the arthritic scores of CIA. The more CD8+ cells in the Peyer's patch, the more CD4+ cells in periphery. High level IFN-gamma and low level TGF-beta in periphery are observed in CIA rats...
Source: Natural Product Research - November 20, 2009 Category: Biochemistry Authors: Xiao C, Zhao L, Liu Z, Lu C, Zhao N, Yang D, Chen S, Tang JC, Chan A, Lu AP Tags: Nat Prod Res Source Type: journals
In vitro cytotoxic effects of Senecio stabianus Lacaita (Asteraceae) on human cancer cell lines.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
This study is aimed to evaluate the in vitro cytotoxicity of Senecio stabianus Lacaita (Asteraceae) against renal adenocarcinoma ACHN, hormone-dependent prostate carcinoma LNCaP, amelanotic melanoma C32 and human breast adenocarcinoma MCF-7 cell lines. The n-hexane extract showed an interesting activity with IC(50) values of 62.7 and 71.1 mug mL(-1) against C32 and LNCaP, respectively. Two compounds identified in the n-hexane extract, linalool and beta-caryophyllene, were found to be active against C32 cells (IC(50) values of 23.2 and 20.1 mug mL(-1), respectively). Among tested constituents, the highest activity was found...
Source: Natural Product Research - November 20, 2009 Category: Biochemistry Authors: Tundis R, Loizzo MR, Bonesi M, Menichini F, Dodaro D, Passalacqua NG, Statti G, Menichini F Tags: Nat Prod Res Source Type: journals
Synthesis, phytotoxic, cytotoxic, acetylcholinesterase and butrylcholinesterase activities of N,N'-diaryl unsymmetrically substituted thioureas.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Fourteen N,N'-diaryl unsymmetrically substituted thioureas were synthesised and their cytotoxic (in vitro), phytotoxic (in vitro), acetylcholinesterase and butrylcholinesterase activities were determined. Thiourea 16 exhibited high, and 1 and 3 showed significant phytotoxic activity. Thioureas 1, 3, 4, 6 and 10 showed significant activity and 2, 6 and 7 indicated moderate cytotoxic activities. Compound 12 exhibited butrylcholinesterase activity higher than a standard reference.
PMID: 19921590 [PubMed - as supplied by publisher] (Source: Natural Product Research)
Source: Natural Product Research - November 20, 2009 Category: Biochemistry Authors: Begum S, Choudhary MI, Khan KM Tags: Nat Prod Res Source Type: journals
In vitro cytotoxic and antioxidative activity of Cornus mas and Cotinus coggygria.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Cytotoxicity and antioxidant properties of the methanol extracts of leaves and flowers of Cornus mas and Cotinus coggygria were studied, together with their chemical composition. Extracts of C. coggygria flowers and leaves showed better antioxidant activity in reaction with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical as well as in inhibition of lipid peroxidation (LP) than C. mas extracts. Preliminary results showed that all extracts possessed potential cytotoxic activity towards HeLa and LS174 human cancer cell lines in vitro, with stronger inhibition against growth of HeLa cell growth than against LS174 cell growth....
Source: Natural Product Research - November 20, 2009 Category: Biochemistry Authors: Savikin K, Zdunic G, Jankovic T, Stanojkovic T, Juranic Z, Menkovic N Tags: Nat Prod Res Source Type: journals
Two new dioxin derivatives from the aerial parts of Lawsonia alba.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
From the aerial parts of Lawsonia alba two new dioxin derivatives, namely 12-[2'-(1', 4'-dioxin-5', 6'-dione)-8''E-undecenyl-dodecanoate (1) and 5-[1'-(docosa-2'E, 5'E-dienyl)]-1,4-dioxin-2,3-dione (2), were isolated and characterised on the basis of spectroscopic methods.
PMID: 19921592 [PubMed - as supplied by publisher] (Source: Natural Product Research)
Source: Natural Product Research - November 20, 2009 Category: Biochemistry Authors: Siddiqui BS, Uddin N, Begum S Tags: Nat Prod Res Source Type: journals
Preparation, characterization and evaluation of biopolymeric gold nanocomposite with antimicrobial activityEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
This report describes the antimicrobial activity of chitosan capped gold nanoparticles coupled with ampicillin (C-AuNp-Amp). C-AuNp-Amp was synthesized using wet precipitation method and characterized using Fourier transform infrared spectroscopy (FT-IR) and atomic force microscopy (AFM) techniques. The optimal level of ampicillin concentration that couples with C-AuNp nanocomposite was determined by using UV-Vis spectroscopy. The agar well diffusion method was used to evaluate the antimicrobial activity and the broth dilution assay was used to determine the minimum inhibitory concentration (MIC). The size of the ellipsoid...
Source: Biotechnology and Applied Biochemistry - November 20, 2009 Category: Biochemistry Authors: M Chamundeeswari, S S. Liji Sobhana, J P. Jacob, M Ganesh Kumar, M Pandima Devi, T P. Sastry, A B. Mandal Source Type: journals
Insulin-stimulated phosphorylation of endothelial nitric oxide synthase at Ser615 contributes to nitric oxide synthesisEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Insulin stimulates endothelial nitric oxide synthesis via protein kinase B/Akt-mediated phosphorylation and activation of endothelial nitric oxide synthase at Ser1177. In previous studies, we have demonstrated that stimulation of endothelial nitric oxide synthase phosphorylation at Ser1177 may be required, yet is not sufficient for insulin-stimulated nitric oxide synthesis. We therefore investigated the role of phosphorylation of endothelial nitric oxide synthase at alternative sites to Ser1177 as candidate parallel mechanisms contributing to insulin-stimulated nitric oxide synthesis. Stimulation of human aortic endothelia...
Source: BJ Signal - November 20, 2009 Category: Biochemistry Authors: S A Ritchie, C F Kohlhaas, A R Boyd, K C Yalla, K Walsh, J MC Connell, I P Salt Tags: BJ Signal Source Type: journals
AR-C155858 is a potent inhibitor of monocarboxylate transporters MCT1 and MCT2 that binds to an intracellular site involving transmembrane helices 7-10Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In this paper we characterise the properties of the potent MCT1 inhibitor, AR-C155858. Inhibitor titrations of L-lactate transport by MCT1 in rat erythrocytes were used to determine the Ki value and number of AR-C155858 binding sites (Et) on MCT1 and the transporter’s turnover number (Kcat). Derived values were 2.3 ± 1.4 nM, 1.29 ± 0.09 nmoles per ml packed cells and 12.2 ± 1.1 s-1 respectively. When expressed in Xenopus laevis oocytes MCT1 and MCT2 were potently inhibited by AR-C155858 whilst MCT4 was not. Inhibition of MCT1 was shown to be time-dependent, and the compound was also active when ...
Source: BJ Energy - November 20, 2009 Category: Biochemistry Authors: M J Ovens, A J Davies, M C Wilson, C M Murray, A P Halestrap Tags: BJ Energy Source Type: journals
Conformations within soluble oligomers and insoluble aggregates revealed by resonance energy transferEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
A fluorescently labeled 20-residue polyglutamic acid peptide E20 was used to study structural changes which occur in E20 as it co-aggregates with other unlabeled polyglutamic acid peptides. Resonance energy transfer was performed using an o-aminobenzamide donor at the N-terminus and 3-nitrotyrosine acceptor at the C-terminus of E20. Polyglutamic acid aggregates were not defined as amyloid, as they were non-fibrillar and did not bind Congo Red. Circular dichroism measurements indicate that polyglutamic acid aggregation involves a transition from [alpha]-helical monomers to aggregated [beta]-sheets. Soluble oligomers are als...
Source: Biopolymers - November 20, 2009 Category: Biochemistry Authors: Jyothi L. Digambaranath, Loan Dang, Monika Dembinska, Andrew Vasyluk, John M. Finke Source Type: journals
'Sulfo-click' for ligation as well as for site-specific conjugation with peptides, fluorophores, and metal chelatorsEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The 'sulfo-click' reaction, which is a chemoselective amidation reaction involving the reaction of an aminoethane sulfonyl azide with a thio acid, encompasses a new approach for ligation and conjugation. Detailed protocols are provided for decorating biologically active peptides or dendrimers with biophysical tags, fluorescent probes, metal chelators, and small peptides by using this reaction as a novel, metal-free 'sulfo-click' approach. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd. (Source: Journal of Peptide Science)
Source: Journal of Peptide Science - November 20, 2009 Category: Biochemistry Authors: Dirk T. S. Rijkers, Remco Merkx, Cheng-Bin Yim, Arwin J. Brouwer, Rob M. J. Liskamp Source Type: journals
An introduction to liquid chromatography-mass spectrometry instrumentation applied in plant metabolomic analysesEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Over the past decade the application of non-targeted high-throughput metabolomic analysis within the plant sciences has gained ever increasing interest and has truly established itself as a valuable tool for plant functional genomics and studies of plant biochemical composition. Whilst proton nuclear magnetic resonance (1H-NMR) spectroscopy is particularly appropriate for the analysis of bulk metabolites and gas chromatography mass spectrometry (GC-MS) to the analysis of volatile organic compounds (VOC's) and derivatised primary metabolites, liquid chromatography (LC)-MS is highly applicable to the analysis of a wide range...
Source: Phytochemical Analysis - November 20, 2009 Category: Biochemistry Authors: J. William Allwood, Royston Goodacre Source Type: journals
Reactions of the Flavin Mononucleotide in Complex I: A Combined Mechanism Describes NADH Oxidation Coupled to the Reduction of APAD+, Ferricyanide, or Molecular OxygenEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 19, 2009 Category: Biochemistry Tags: article Source Type: journals
Kinetic Analysis of Cysteine Desulfurase CD0387 from Synechocystis sp. PCC 6803: Formation of the Persulfide IntermediateEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 19, 2009 Category: Biochemistry Tags: article Source Type: journals
Evidence for the Involvement of Human DNA Polymerase N in the Repair of DNA Interstrand Cross-LinksEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 19, 2009 Category: Biochemistry Tags: article Source Type: journals
Physical Instability of a Therapeutic Fc Fusion Protein: Domain Contributions to Conformational and Colloidal StabilityEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 19, 2009 Category: Biochemistry Tags: article Source Type: journals
Post-Translational Modifications to Toxoplasma gondii α- and β-Tubulins Include Novel C-Terminal MethylationEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Journal of Proteome Research, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Journal of Proteome Research)
Source: Journal of Proteome Research - November 19, 2009 Category: Biochemistry Tags: article Source Type: journals
Oligomeric Characterization of the Photosynthetic Apparatus of Rhodobacter sphaeroides R26.1 by Nondenaturing Electrophoresis MethodsEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Journal of Proteome Research, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Journal of Proteome Research)
Source: Journal of Proteome Research - November 19, 2009 Category: Biochemistry Tags: article Source Type: journals
Overexpression Of Sodium/Calcium Exchanger Protein Alone Did Not Cause Heart FailureEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Researchers from the Center for Translational Medicine at Thomas Jefferson University have found that the overexpression of a sodium/calcium exchanger, without changes in other ion transport pathways commonly observed in heart failure, does not by itself lead to contraction abnormalities in the heart. They presented the data from the study at the American Heart Association Scientific Sessions in Orlando, Fla. Led by Joseph Cheung, M.D., Ph.D. (Source: Biology / Biochemistry News From Medical News Today)
Source: Biology / Biochemistry News From Medical News Today - November 19, 2009 Category: Biochemistry Tags: Heart Disease Source Type: news
Scientific Link To Autism IdentifiedEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
During its research into the application of neuroscience in business, a New Jersey based think tank, The Center for Modeling Optimal Outcomes®, LLC (The Center) made an inadvertent and amazing discovery. The Center examined the neuroscientific dynamics of logic and emotion in decision making while researching neuroscience in business. They found unique corollary relationships between various brain chemicals (neurohormones, neurotransmitters, etc.). (Source: Biology / Biochemistry News From Medical News Today)
Source: Biology / Biochemistry News From Medical News Today - November 19, 2009 Category: Biochemistry Tags: Autism Source Type: news
The novel ruthenium - [gamma]-linolenic complex [Ru2(aGLA)4Cl] inhibits C6 rat glioma cell proliferation and induces changes in mitochondrial membrane potential, increased reactive oxygen species generation and apoptosis in vitroEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The present study reports the synthesis of a novel compound with the formula [Ru2(aGLA)4Cl] according to elemental analyses data, referred to as Ru2GLA. The electronic spectra of Ru2GLA is typical of a mixed valent diruthenium(II,III) carboxylate. Ru2GLA was synthesized with the aim of combining and possibly improving the anti-tumour properties of the two active components ruthenium and [gamma]-linolenic acid (GLA). The properties of Ru2GLA were tested in C6 rat glioma cells by analysing cell number, viability, lipid droplet formation, apoptosis, cell cycle distribution, mitochondrial membrane potential and reactive oxygen...
Source: Cell Biochemistry and Function - November 19, 2009 Category: Biochemistry Authors: Geise Ribeiro, Marcel Benadiba, Denise de Oliveira Silva, Alison Colquhoun Source Type: journals
Intermolecular cross-linking of monomers in H. pylori Na{+}/H{+} antiporter NhaA at dimer interface inhibits antiporter activityEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
We previously showed that the Na+/H+ antiporter of Helicobacter pylori (HPNhaA) forms an oligomer in a native membrane of Escherichia coli, and conformational changes of oligomer occur between monomers of the oligomer during ion transport. In the current study, we use Blue Native PAGE to show that HPNhaA forms a dimer. Cysteine-scanning mutagenesis of residues 55 to 61 in a putative β-sheet region of loop1 and subsequent functional analyses revealed that the Q58C mutation resulted in an intermolecular disulfide bond. G56C, I59C, and G60C were found to be cross-linked by bifunctional cross-linkers. Furt...
Source: BJ Energy - November 19, 2009 Category: Biochemistry Authors: A Karasawa, K Mitsui, M Matsushita, H Kanazawa Tags: BJ Energy Source Type: journals
Phosphatome profiling reveals PTPN2, PTPRJ and PTEN as potent negative regulators of PKB/Akt activation in Ras mutated cancer cellsEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Oncogenic Ras mutations render the protein constitutively active and promote tumourigenisis via chronic stimulation of effector pathways. In A549 lung adenocarcinoma approximately 50% of the total Ras population is constitutively active yet these cells display only weak activation of the effectors: ERK1/2 and Akt. In order to identify key negative regulators of oncogenic Ras signalling we performed a phosphatome RNAi screen in A549 cells and ranked their effects on phosphorylation of Ser473 of Akt. As expected, the tumour suppressor PTEN emerged as a leading hit – knockdown elevated Akt activation to 70% of maximal ...
Source: BJ Disease - November 19, 2009 Category: Biochemistry Authors: J Omerovic, M J. Clague, I A. Prior Tags: BJ Signal Source Type: journals
MSK1 regulates the transcription of IL-1ra in response to TLR activation in macrophages.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The activity of the pro-inflammatory cytokine IL-1 is closely regulated in vivo via a variety of mechanisms, including both the control of IL-1 production and secretion as well as naturally occurring inhibitors of IL-1 function such as IL-1ra. IL-1ra is homologous to IL-1, and is able to bind but not activate the IL-1 receptor. IL-1ra can be produced by a variety of cell types, and its production is stimulated by inflammatory signals. Here we show that in macrophages the TLR mediated induction of IL-1ra from both its proximal and distal promoters involves the p38 and ERK1/2 MAPK cascades. In addition we show that mitogen a...
Source: BJ Disease - November 19, 2009 Category: Biochemistry Authors: J Darragh, O Ananieva, A Courtney, S Elcombe, J C Arthur Tags: BJ Signal Source Type: journals
CFTR fragments with the F508 deletion exert a dual allosteric control over the master kinase CK2.Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Cystic Fibrosis (CF) mostly follows a single F508 deletion in CFTR (ΔF508), thereby causing premature fragmentation of the nascent protein with concomitant alterations of diverse cellular functions. We show that CK2, the most pleiotropic protein kinase, undergoes allosteric control of its different cellular forms in the presence of short CFTR peptides encompassing the F508 deletion: these ΔF508 peptides drastically inhibit the isolated catalytic subunit (α) of the kinase and yet up-regulate the holoenzyme, composed of two catalytic and two non catalytic (β) subunits. Remarkable agreement between...
Source: BJ Disease - November 19, 2009 Category: Biochemistry Authors: M A Pagano, O Marin, G Cozza, S Sarno, F Meggio, K J. Treharne, A Mehta, L A Pinna Tags: BJ Signal Source Type: journals
The highly conserved synapsin domain E mediates synapsin dimerization and phospholipid vesicle clusteringEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Synapsins are abundant synaptic vesicle (SV)-associated phosphoproteins that regulate synapse formation and function. The highly conserved COOH-terminal domain E was shown to contribute to several synapsin functions, ranging from formation of the SV reserve pool to regulation of the kinetics of exocytosis and SV cycling, although the molecular mechanisms underlying these effects are unknown. We used a synthetic 25-mer peptide encompassing the most conserved region of domain E (Pep-E) to analyze the role of domain E in regulating the interactions between synapsin I and liposomes mimicking the phospholipid composition of SVs...
Source: BJ Cell - November 19, 2009 Category: Biochemistry Authors: I Monaldi, M Vassalli, A Bachi, S Giovedi, E Millo, F Valtorta, R Raiteri, F Benfenati, A Fassio Tags: BJ Cell Source Type: journals
Paraoxonase 2 is downregulated by the Pseudomonas aeruginosa quorum sensing signal N-(3-oxododecanoyl)-L-homoserine lactone and attenuates oxidative stress induced by pyocyaninEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Two virulence factors produced by Pseudomonas aeruginosa are pyocyanin and N-(3-oxododecanoyl)-L-homoserine lactone (3OC12). Pyocyanin damages host cells by generating reactive oxygen species (ROS). 3OC12 is a quorum-sensing signaling molecule which regulates bacterial gene expression and modulates host immune responses. Paraoxonase-2 (PON2) is an esterase that inactivates 3OC12 and potentially attenuates P. aeruginosa virulence. Because increased intracellular calcium initiates the degradation of PON2 mRNA and protein and 3OC12 causes increases in cytosolic calcium, we hypothesized that 3OC12 would also downregulate PON2....
Source: BJ Cell - November 19, 2009 Category: Biochemistry Authors: S Horke, I Witte, S Altenhöfer, P Wilgenbus, M Goldeck, U Förstermann, J Xiao, G L Kramer, D C Haines, P K Chowdhary, R W Haley, J F Teiber Tags: BJ Disease Source Type: journals
MCP-1 causes cardiomyoblast death via autophagy resulting from ER stress caused by oxidative stress generated by inducing a novel Zn-finger protein, MCPIPEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
We present evidence that MCP-1 causes death in cardiac myoblasts, H9c2, by inducing oxidative stress that causes ER stress leading to autophagy via a novel Zn-finger protein, MCP-1 induced protein (MCPIP). MCPIP expression caused cell death and knockdown of MCPIP attenuated MCP-1 induced cell death. It caused induction of iNOS, NADPH oxidase subunit phox47, its translocation from the cytoplasm to the membrane and production of reactive oxygen species (ROS), and induction of ER stress markers HSP40, PDI, GRP78 and IRE1α. It also caused autophagy as indicated by beclin-1 induction, cleavage of LC3 and autophagolysosom...
Source: BJ Cell - November 19, 2009 Category: Biochemistry Authors: C W. Younce, P E Kolattukudy Tags: BJ Signal Source Type: journals
Antioxidant activities of curcumin and combinations of this curcuminoid with other phytochemicalsEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
In conclusion, curcumin is a potent antioxidant in a reaction that may be relevant to in vivo toxicity. In relation to two other well-known antioxidants, curcumin shows significantly greater synergism with resveratrol than with quercetin. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Phytotherapy Research)
Source: Phytotherapy Research - November 19, 2009 Category: Biochemistry Authors: N. Aftab, A. Vieira Source Type: journals
Inhibitory effect of the cree traditional medicine wiishichimanaanh (Vaccinium vitis-idaea) on advanced glycation endproduct formation: identification of active principlesEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Like many aboriginal populations, First Nations communities such as the Cree of Eeyou Istchee are facing continuously increasing rates of diabetes and related complications. Advanced glycation endproducts (AGEs), which readily form and accumulate with sustained hyperglycemia, contribute to the development of diabetic complications and, as such, are considered a potential therapeutic target. In the present study, the inhibition of AGE formation by ethanolic extracts of the Cree medicinal plant Vaccinium vitis-idaea L. was assessed by fluorometric detection of fluorescent AGEs and immunodetection of N[egr]-(carboxymethyl)lys...
Source: Phytotherapy Research - November 19, 2009 Category: Biochemistry Authors: Louis-Philippe Beaulieu, Cory S. Harris, Ammar Saleem, Alain Cuerrier, Pierre S. Haddad, Louis C. Martineau, Steffany A.L. Bennett, John T. Arnason Source Type: journals
Effects of Athamanta turbith fruit essential oils on CCl4-induced hepatic failure in mice and their antioxidant propertiesEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
The effects of essential oils isolated from mature fruits of Athamanta turbith ssp. hungarica (Borbás) Tutin and A. turbith ssp. haynaldii (Borbás & Uechtr.) Tutin (Umbelliferae) on some liver biochemical parameters in mice intoxicated with carbon tetrachloride were investigated. Pretreatment with both essential oils extenuated the effects caused by carbon tetrachloride. In order to investigate in vitro antioxidant properties of the oils, three methods were applied: scavenging of both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH radicals, as well as a test of inhibition of Fe2+/ascorbic-induced lipid peroxidation. Investi...
Source: Phytotherapy Research - November 19, 2009 Category: Biochemistry Authors: Tomi[cacute] Ana, Bo[zcaron]in Biljana, Samojlik Isidora, Milenkovi[cacute] Marina, Mimica-Duki[cacute] Neda, Petrovi[cacute] Silvana Source Type: journals
Association of Phosphatidic Acid with the Bovine Mitochondrial ADP/ATP CarrierEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 18, 2009 Category: Biochemistry Tags: article Source Type: journals
Characterization of a Key Neutralizing Epitope on Pertussis Toxin Recognized by Monoclonal Antibody 1B7Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 18, 2009 Category: Biochemistry Tags: article Source Type: journals
Mansouramycins A−D, Cytotoxic Isoquinolinequinones from a Marine Streptomycete(1)Email this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Journal of Natural Products, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Journal of Natural Products)
Source: Journal of Natural Products - November 18, 2009 Category: Biochemistry Tags: article Source Type: journals
MSQuant, an Open Source Platform for Mass Spectrometry-Based Quantitative ProteomicsEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Journal of Proteome Research, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Journal of Proteome Research)
Source: Journal of Proteome Research - November 18, 2009 Category: Biochemistry Tags: article Source Type: journals
Insight into the Mechanism of Inactivation of Ribonucleotide Reductase by Gemcitabine 5′-Diphosphate in the Presence or Absence of ReductantEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 18, 2009 Category: Biochemistry Tags: article Source Type: journals
Distinct Pose of Discodermolide in Taxol Binding Pocket Drives a Complementary Mode of Microtubule StabilizationEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 18, 2009 Category: Biochemistry Tags: article Source Type: journals
Mechanism of Inactivation of Human Ribonucleotide Reductase with p53R2 by Gemcitabine 5′-DiphosphateEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 18, 2009 Category: Biochemistry Tags: article Source Type: journals
Heme Iron Nitrosyl Complex of MauG Reveals an Efficient Redox Equilibrium between Hemes with Only One Heme Exclusively Binding Exogenous LigandsEmail this article to a colleague.
Save this article to My Clippings.
Discuss or comment on this article.
Biochemistry, Volume 0, Issue 0, Articles ASAP (As Soon As Publishable). (Source: Biochemistry)
Source: Biochemistry - November 18, 2009 Category: Biochemistry Tags: article Source Type: journals
