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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 16.

Blastocystis: Pathogen or Passenger?
An Evaluation of 101 Years of Researchseries:Parasitology Research MonographsThe stages of Blastocystis have been known for 101 years. However, many facts are still disputed, e.g. even the question whether it is a true pathogen or a commensal present in sometimes life-threatening diarrheas. The present book evaluates in chapters contributed by renowned researchers the latest findings on:•Landmarks in the discovery of Blastocystis•Epidemiology, transmission and zoonotic ...
Source: Springer Biomedical Sciences titles - December 14, 2012 Category: Biomedical Science Tags: Parasitology Source Type: news

Protein Quality Control in Neurodegenerative Diseases
series:Research and Perspectives in Alzheimer's DiseaseThe health of the proteome depends upon protein quality control to regulate the proper synthesis, folding., translocation, and clearance of proteins. The cell is challenged constantyl by environmental and physiological stress, aging, and the chronic expressions of disease associated misfolded proteins. Substantial evidence supports the hypothesis that the expression of damaged proteins ...
Source: Springer Biomedical Sciences titles - December 14, 2012 Category: Biomedical Science Tags: Neurosciences Source Type: news

Biomedical Applications of Peptide-, Glyco- and Glycopeptide Dendrimers, and Analogous Dendrimeric Structures(Sebestik et al.)
Source: Springer Biomedical Sciences titles - December 14, 2012 Category: Biomedical Science Tags: Molecular Medicine Source Type: news

Engineering superficial zone features in tissue engineered cartilage
Abstract A major challenge in cartilage tissue engineering is the need to recreate the native tissue's anisotropic extracellular matrix structure. This anisotropy has important mechanical and biological consequences and could be crucial for integrative repair. Here we report that hydrodynamic conditions that mimic the motion‐induced flow fields in between the articular surfaces in the synovial joint induce the formation of a distinct superficial layer in tissue engineered cartilage hydrogels, with enhanced production of cartilage matrix proteoglycan and type II collagen. Moreover, the flow stimulation at the surface indu...
Source: Biotechnology and Bioengineering - December 14, 2012 Category: Biomedical Science Authors: Tony Chen, Matthew J. Hilton, Edward B. Brown, Michael J. Zuscik, Hani A. Awad Tags: Article Source Type: research

Muscle contributions to fore-aft and vertical body mass center accelerations over a range of running speeds
Abstract: Running is a bouncing gait in which the body mass center slows and lowers during the first half of the stance phase; the mass center is then accelerated forward and upward into flight during the second half of the stance phase. Muscle-driven simulations can be analyzed to determine how muscle forces accelerate the body mass center. However, muscle-driven simulations of running at different speeds have not been previously developed, and it remains unclear how muscle forces modulate mass center accelerations at different running speeds. Thus, to examine how muscles generate accelerations of the body mass center, we...
Source: Journal of Biomechanics - December 14, 2012 Category: Biomedical Science Authors: Samuel R. Hamner, Scott L. Delp Tags: Papers Source Type: research

Patient-specific finite-element simulation of the human cornea: A clinical validation study on cataract surgery
Abstract: The planning of refractive surgical interventions is a challenging task. Numerical modeling has been proposed as a solution to support surgical intervention and predict the visual acuity, but validation on patient specific intervention is missing. The purpose of this study was to validate the numerical predictions of the post-operative corneal topography induced by the incisions required for cataract surgery.The corneal topography of 13 patients was assessed preoperatively and postoperatively (1-day and 30-day follow-up) with a Pentacam tomography device. The preoperatively acquired geometric corneal topography �...
Source: Journal of Biomechanics - December 14, 2012 Category: Biomedical Science Authors: Harald P. Studer, Hansjörg Riedwyl, Christoph A. Amstutz, James V.M. Hanson, Philippe Büchler Tags: Papers Source Type: research

How do metacarpophalangeal joint extension, collateromotion and axial rotation influence dorsal surface strains of the equine proximal phalanx at different loads in vitro?
Abstract: The biomechanical circumstances that promote sagittal fracture of the equine proximal phalanx (P1) are poorly understood. In order to improve our understanding of equine metacarpophalangeal joint (MCPJ) biomechanics and potential aetiologies of sagittal P1 fractures, the study objectives were to quantify P1 bone strains, collateromotion and axial rotation during MCPJ extension under controlled loading circumstances. Unilateral limbs from six cadavers were instrumented with bone reference markers for measurement of P1 movement relative to third metacarpal bone positions during axial limb loading to 10,500N. Bone r...
Source: Journal of Biomechanics - December 14, 2012 Category: Biomedical Science Authors: Ellen Singer, Tanya Garcia, Susan Stover Tags: Papers Source Type: research

Normal aging alters in vivo passive biomechanical response of the rat gastrocnemius-Achilles muscle–tendon unit
Abstract: Predisposition to Achilles tendon (AT) ruptures in middle-aged individuals may be associated with age-related changes to inherent passive biomechanical properties of the gastrocnemius-Achilles (GC-AT) muscle–tendon unit, due to known muscle–tendon structural changes in normal aging. The goal of this study was to determine whether the passive biomechanical response of the GC-AT muscle–tendon unit was altered with age in 6 young (8 months) and 6 middle-aged (24 months) F344xBN hybrid rats from the National Institute on Aging colony. Fung's quasilinear viscoelastic (QLV) model was used to determine in vivo his...
Source: Journal of Biomechanics - December 14, 2012 Category: Biomedical Science Authors: Johannes F. Plate, Walter F. Wiggins, Patrick Haubruck, Aaron T. Scott, Thomas L. Smith, Katherine R. Saul, Sandeep Mannava Tags: Papers Source Type: research

The kinetic properties of a human PPIP5K reveal that its kinase activities are protected against the consequences of a deteriorating cellular bioenergetic environment.
Abstract We obtained detailed kinetic characteristics - stoichiometry, reaction rates, substrate affinities, and equilibrium conditions - of human PPIP5K2, which synthesizes "high-energy" diphosphoinositol polyphosphates (PP-InsPs) by metabolizing InsP6 and 5-InsP7 to 1-InsP7 and InsP8, respectively. These data increase our insight into the PPIP5K2 reaction mechanism and clarify the interface between PPIP5K catalytic activities and cellular bio-energetic status. For example, stochiometric analysis uncovered non-productive, substrate-stimulated ATPase activity (thus, approximately 2 and 1.2 ATP molecules are utilize...
Source: Bioscience Reports - December 14, 2012 Category: Biomedical Science Authors: Weaver JD, Wang H, Shears SB Tags: Biosci Rep Source Type: research

Bioactive polyacrylamide hydrogels with gradients in mechanical stiffness
Abstract We propose a novel, single step method for the production of polyacrylamide hydrogels with a gradient in mechanical properties. In contrast to already existing techniques such as UV photo‐polymerization with photomasks (limited penetration depth) or microfluidic gradient mixers (complex microfluidic chip), this technique is not suffering such limitations. Young's modulus of the hydrogels was varied by changing the total monomer concentration of the hydrogel precursor solution. Using programmable syringe pumps, the total monomer concentration in the solution fed to the hydrogel mold was varied from 16 wt% down to...
Source: Biotechnology and Bioengineering - December 14, 2012 Category: Biomedical Science Authors: Vincent E.G. Diederich, Peter Studer, Anita Kern, Marco Lattuada, Giuseppe Storti, Ram I. Sharma, Jess G. Snedeker, Massimo Morbidelli Tags: Article Source Type: research

Programmable mechanical stimulation influences tendon homeostasis in a bioreactor system
Abstract Identification of functional programmable mechanical stimulation (PMS) on tendon not only provides the insight of the tendon homeostasis under physical/pathological condition, but also guides a better engineering strategy for tendon regeneration. The aims of the study are to design a bioreactor system with PMS to mimic the in vivo loading conditions, and to define the impact of different cyclic tensile strain on tendon. Rabbit Achilles tendons were loaded in the bioreactor with/without cyclic tensile loading (0.25Hz for 8 hours/day, 0∼9% for 6 days). Tendons without loading lost its structure integrity as eviden...
Source: Biotechnology and Bioengineering - December 14, 2012 Category: Biomedical Science Authors: Tao Wang, Zhen Lin, Robert E Day, Bruce Gardiner, Euphemie Landao‐Bassonga, Jonas Rubenson, Thomas B. Kirk, David W. Smith, David G. Lloyd, Gerard Hardisty, Allan Wang, Qiujian Zheng, Ming H Zheng Tags: Article Source Type: research

Development of a new bioprocess scheme using frozen seed train intermediates to initiate CHO cell culture manufacturing campaigns
Abstract Agility to schedule and execute cell culture manufacturing campaigns quickly in a multi‐product facility will play a key role in meeting the growing demand for therapeutic proteins. In an effort to shorten campaign timelines, maximize plant flexibility and resource utilization, we investigated the initiation of cell culture manufacturing campaigns using CHO cells cryopreserved in large volume bags in place of the seed train process flows that are conventionally used in cell culture manufacturing. This approach, termed FASTEC (Frozen Accelerated Seed Train for Execution of a Campaign), involves cultivating cells ...
Source: Biotechnology and Bioengineering - December 14, 2012 Category: Biomedical Science Authors: Gargi Seth, Robert W. Hamilton, Thomas R. Stapp, Lisa Zheng, Angela Meier, Krista Petty, Stephenie Leung, Srikanth Chary Tags: Article Source Type: research

Orthogonal control of endogenous gene expression in mammalian cells using synthetic ligands
Abstract Gene switches have wide utility in synthetic biology, gene therapy, and developmental biology, and multiple orthogonal gene switches are needed to construct advanced circuitry or to control complex phenotypes. Endogenous vascular endothelial growth factor (VEGF‐A) is crucial to angiogenesis, and it has been shown that multiple alternately spliced VEGF‐A isoforms are necessary for proper blood vessel formation. Such a necessity limits the utility of direct transgene delivery, which can provide only one splice variant. To overcome this limitation, we constructed a gene switch that can regulate the (VEGF‐A) loc...
Source: Biotechnology and Bioengineering - December 14, 2012 Category: Biomedical Science Authors: Jing Liang, Michael J. McLachlan, Huimin Zhao Tags: Article Source Type: research

New neuroscience textbook will be a free reference for students in developing countries
The textbook, conceived and edited by Rockefeller University professor Donald W. Pfaff, is a 3,200 page, five-volume overview of both basic science and clinical issues in modern neuroscience, aimed at premedical, medical and graduate students. More »
Source: The Rockefeller University Newswire - December 13, 2012 Category: Biomedical Science Authors: pubaff Tags: Campus News Source Type: news

Quintiles to commercialize drug in Europe
Quintiles will commercialize a drug to treat diabetic macular edema in Europe. The drug, Iluvien, is produced by Atlanta-based Alimera Sciences Inc. (Nasdaq: ALIM), which inked a deal with Quintiles Commercial Europe Ltd., a subsidiary of Quintiles, in late November. Quintiles will handle marketing, brand managing and sales promotions and also work as a regulatory and medical science liaison in European countries where the drug will be sold. Financial details were not disclosed. Alimera is a development…
Source: bizjournals.com Health Care:Physician Practices headlines - December 13, 2012 Category: American Health Authors: Jason deBruyn Source Type: research

Pharmacokinetics and brain uptake in the Rhesus monkey of a fusion protein of arylsulfatase A and a monoclonal antibody against the human insulin receptor
Abstract Metachromatic leukodystrophy (MLD) is a lysosomal storage disorder of the brain caused by mutations in the gene encoding the lysosomal sulfatase, arylsulfatase A (ASA). It is not possible to treat the brain in MLD with recombinant ASA, because the enzyme does not cross the blood‐brain barrier (BBB). In the present investigation, a BBB‐penetrating IgG‐ASA fusion protein is engineered and expressed, where the ASA monomer is fused to the carboxyl terminus of each heavy chain of an engineered monoclonal antibody (MAb) against the human insulin receptor (HIR). The HIRMAb crosses the BBB via receptor‐mediated tr...
Source: Biotechnology and Bioengineering - December 13, 2012 Category: Biomedical Science Authors: Ruben J. Boado, Jeff Zhiqiang Lu, Eric K.‐W. Hui, Rachita K. Sumbria, William M. Pardridge Tags: Article Source Type: research

A Picture is Worth a Thousand Tables
Graphics in Life SciencesGraphics promote scientific insight, better business and more effective communication. However, in the Life Sciences, drug development processes are still largely reliant on tables and listings.This book sets out to change this situation. The primary aim of the volume is to provide a rich collection of examples showing graphical review and reporting across the Life Sciences industry. Medical ...
Source: Springer Biomedical Sciences titles - December 13, 2012 Category: Biomedical Science Tags: Biomedicine (general) Source Type: news

MiR-190 leads to aggressive phenotype of neuroblastoma through indirect activation of TrkB pathway
Neuroblastoma (NBL) is one of the most common solid tumors in children. Almost one third of all children with advanced stage NBL is incurable, and NBL accounts for approximately 15% of all childhood cancer deaths. Currently, treatment of NBL patients is tailored to risk group assignment according to well-known prognostic factors in addition to MYCN amplification, such as patient age at diagnosis, International Neuroblastoma Staging System (INSS) stage, tumor histopathology, and DNA index .
Source: Medical Hypotheses - December 13, 2012 Category: Biomedical Science Authors: Ondrej Slaby Tags: Correspondence Source Type: research

Association of birthdays with distant relapses of immune thrombocytopenia
In this study, the possibility that birthdays might be associated with initial presentations of or with distant relapses of ITP was explored.
Source: Medical Hypotheses - December 13, 2012 Category: Biomedical Science Authors: Mala Varma Tags: Correspondence Source Type: research

Smart bandages – A colourful approach to early stage infection detection & control in wound care
Abstract: With the recent increase in bacterial resistance to conventional antibiotics, the early-stage detection and control of infection has become imperative in the fight against opportunistic pathogens in healthcare. The traditional β-lactam wonder-drugs (e.g. penicillin and cephalosporins), are rendered inactive due to enzymatic hydrolysis by bacterial β-lactamase enzymes as a bacterial defence mechanism. However, this deactivation mechanism produces different responses in the two aforementioned drugs – with the cephalosporins showing a molecular rearrangement mechanism which could be utilised for prodrug delivery...
Source: Medical Hypotheses - December 13, 2012 Category: Biomedical Science Authors: Kayleigh L. Brocklesby, Stephen C. Johns, Allison E. Jones, Duncan Sharp, Robert B. Smith Tags: Articles Source Type: research

Anatomically reproducible assessment of volumetric bone mineral density — Based on clinical computed tomography
Abstract: Local distribution and quality of bone mineral density (BMD) in the proximal humerus are important for rotator cuff repair, internal fixations, and arthroplasty. The current study aimed to develop a volumetric BMD (vBMD) measurement technique that can specify the volumetric region of interest in an anatomically reproducible manner and ensures fair comparison of vBMD among intrasubjective or intersubjective measurements. Computed toed into three-dimensional (3D) polygon models. A standardized volumetric block was asmographic images of 10 intact shoulders (from six women and four men, age range 46–69 years) were ...
Source: Journal of Biomechanics - December 13, 2012 Category: Biomedical Science Authors: Yeon Soo Lee, Terihisa Mihata, Joo Han Oh Tags: Papers Source Type: research

How does epidermal pathology interact with mental state?
Abstract: The hypothesis is presented that human emotional state is influenced by epidermal pathology via the release from epidermal keratinocytes of a wide variety of chemical mediators (including neurotransmitters) that act on the brain. It has long been recognized that epidermal keratinocytes play a key role in the function of the stratum corneum as an impermeable barrier, and that skin diseases such as atopic dermatitis and psoriasis, which cause itching, sleep disturbance and concern over appearance, are associated with depression and anxiety. On the other hand, epidermal keratinocytes are known to produce and release...
Source: Medical Hypotheses - December 13, 2012 Category: Biomedical Science Authors: Mitsuhiro Denda, Kentaro Takei, Sumiko Denda Tags: Articles Source Type: research

Ribonucleotide reductase and thymidylate synthase or exogenous deoxyribonucleosides reduce DNA damage and senescence caused by C-MYC depletion.
Abstract The down-regulation of dominant oncogenes, including C-MYC, in tumor cells often leads to the induction of senescence via mechanisms that are not completely identified. In the current study, we demonstrate that MYC-depleted melanoma cells undergo extensive DNA damage that is caused by the underexpression of thymidylate synthase (TS) and ribonucleotide reductase (RR) and subsequent depletion of deoxyribonucleoside triphosphate pools. Simultaneous genetic inhibition of TS and RR in melanoma cells induced DNA damage and senescence phenotypes very similar to the ones caused by MYC-depletion. Reciprocally, over...
Source: Aging - December 13, 2012 Category: Biomedical Science Authors: Mannava S, Moparthy KC, Wheeler LJ, Leonova KI, Wawrzyniak JA, Bianchi-Smiraglia A, Berman AE, Flanagan S, Shewach DS, Zeitouni NC, Gudkov AV, Mathews CK, Nikiforov MA Tags: Aging (Albany NY) Source Type: research

Multiscale analysis of genome-wide replication timing profiles using a wavelet-based signal-processing algorithm
Nature Protocols 8, 98 (2013). doi:10.1038/nprot.2012.145 Authors: Benjamin Audit, Antoine Baker, Chun-Long Chen, Aurélien Rappailles, Guillaume Guilbaud, Hanna Julienne, Arach Goldar, Yves d'Aubenton-Carafa, Olivier Hyrien, Claude Thermes & Alain Arneodo In this protocol, we describe the use of the LastWave open-source signal-processing command language (http://perso.ens-lyon.fr/benjamin.audit/LastWave/) for analyzing cellular DNA replication timing profiles. LastWave makes use of a multiscale, wavelet-based signal-processing algorithm that is based on a rigorous theoretical analysis linking timing profiles to
Source: Nature Protocols - December 13, 2012 Category: Biomedical Science Authors: Benjamin AuditAntoine BakerChun-Long ChenAurélien RappaillesGuillaume GuilbaudHanna JulienneArach GoldarYves d'Aubenton-CarafaOlivier HyrienClaude ThermesAlain Arneodo Tags: Protocol Source Type: research

A method to resolve the composition of heterogeneous affinity-purified protein complexes assembled around a common protein by chemical cross-linking, gel electrophoresis and mass spectrometry
Nature Protocols 8, 75 (2013). doi:10.1038/nprot.2012.133 Authors: Elena L Rudashevskaya, Roberto Sacco, Klaus Kratochwill, Marie L Huber, Matthias Gstaiger, Giulio Superti-Furga & Keiryn L Bennett Protein complexes form, dissociate and re-form in order to perform specific cellular functions. In this two-pronged protocol, noncovalent protein complexes are initially isolated by affinity purification for subsequent identification of the components by liquid chromatography high-resolution mass spectrometry (LC-MS) on a hybrid LTQ Orbitrap Velos.
Source: Nature Protocols - December 13, 2012 Category: Biomedical Science Authors: Elena L RudashevskayaRoberto SaccoKlaus KratochwillMarie L HuberMatthias GstaigerGiulio Superti-FurgaKeiryn L Bennett Tags: Protocol Source Type: research

Ex vivo perfusion of mid-to-late-gestation mouse placenta for maternal-fetal interaction studies during pregnancy
Nature Protocols 8, 66 (2013). doi:10.1038/nprot.2012.144 Authors: Nick Goeden & Alexandre Bonnin Ex vivo perfusion systems offer a reliable, reproducible method for studying acute physiological responses of an organ to various environmental manipulations. Unlike in vitro culture systems, the cellular organization, compartmentalization and three-dimensional structure of ex vivo–perfused organs are maintained. These particular parameters
Source: Nature Protocols - December 13, 2012 Category: Biomedical Science Authors: Nick GoedenAlexandre Bonnin Tags: Protocol Source Type: research

Surface analysis using shell-isolated nanoparticle-enhanced Raman spectroscopy
Nature Protocols 8, 52 (2013). doi:10.1038/nprot.2012.141 Authors: Jian Feng Li, Xiang Dong Tian, Song Bo Li, Jason R Anema, Zhi Lin Yang, Yong Ding, Yuan Fei Wu, Yong Ming Zeng, Qi Zhen Chen, Bin Ren, Zhong Lin Wang & Zhong Qun Tian Surface-enhanced Raman scattering (SERS) is a powerful fingerprint vibrational spectroscopy with a single-molecule detection limit, but its applications are generally restricted to 'free-electron–like' metal substrates such as Au, Ag and Cu nanostructures. We have invented a shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS) technique,
Source: Nature Protocols - December 13, 2012 Category: Biomedical Science Authors: Jian Feng LiXiang Dong TianSong Bo LiJason R AnemaZhi Lin YangYong DingYuan Fei WuYong Ming ZengQi Zhen ChenBin RenZhong Lin WangZhong Qun Tian Tags: Protocol Source Type: research

Homogenization of Pseudomonas aeruginosa PAO1 biofilms visualized by freeze‐substitution electron microscopy
Abstract A knowledge of the mechanical properties of bacterial biofilms is required to more fully understand the processes of biofilm formation such as initial adhesion or detachment. The main contribution of this paper is to demonstrate the use of homogenization techniques to compute mechanical parameters of Pseudomonas aeruginosa PAO1 biofilms. For this purpose, homogenization techniques are used to analyze freeze substitution electron micrographs of the biofilm cross‐sections. The concept of a representative volume element and the study about his representativeness allows us to determine the optimal size in order to a...
Source: Biotechnology and Bioengineering - December 13, 2012 Category: Biomedical Science Authors: T. Guélon, R.C. Hunter, J. D. Mathias, G. Deffuant Tags: Article Source Type: research

Real‐time detection of cellular death receptor‐4 activation by fluorescence resonance energy transfer
Abstract Targeted therapy involving the activation of death receptors DR4 and/or DR5 by its ligand, TRAIL, can selectively induce apoptosis in certain tumor cells. In order to profile the dynamic activation or trimerization of TRAIL‐DR4 in live cells in real time, the development of an apoptosis reporter cell line is essential. Fluorescence resonance energy transfer (FRET) technology via a FRET pair, cyan fluorescence protein (CFP) and yellow fluorescence protein (YFP), was used in this study. DR4‐CFP and DR4‐YFP were stably expressed in human lung cancer PC9 cells. Flow cytometer sorting and limited dilution coupled...
Source: Biotechnology and Bioengineering - December 13, 2012 Category: Biomedical Science Authors: Zeynep Dereli‐Korkut, Harmeet Gandhok, Ling Ge Zeng, Sidra Waqas, Xuejun Jiang, Sihong Wang Tags: Article Source Type: research

Permeability of a growing biofilm in a porous media fluid flow analyzed by magnetic resonance displacement‐relaxation correlations
Abstract Biofilm growth in porous media is difficult to study non‐invasively due to the opaqueness and heterogeneity of the systems. Magnetic resonance is utilized to non‐invasively study water dynamics within porous media. Displacement‐relaxation correlation experiments were performed on fluid flow during biofilm growth in a model porous media of mono‐dispersed polystyrene beads. The spin‐spin T2 magnetic relaxation distinguishes between the biofilm phase and bulk fluid phase due to water ‐ biopolymer interactions present in the biofilm, and the flow dynamics are measured using PGSE NMR experiments. By correla...
Source: Biotechnology and Bioengineering - December 13, 2012 Category: Biomedical Science Authors: Sarah J. Vogt, Alexis B. Sanderlin, Joseph D. Seymour, Sarah L. Codd Tags: Article Source Type: research

Stem cell microencapsulation for phenotypic control, bioprocessing, and transplantation
Abstract Cell microencapsulation has been utilized for decades as a means to shield cells from the external environment while simultaneously permitting transport of oxygen, nutrients, and secretory molecules. In designing cell therapies, donor primary cells are often difficult to obtain and expand to appropriate numbers, rendering stem cells an attractive alternative due to their capacities for self‐renewal, differentiation, and trophic factor secretion. Microencapsulation of stem cells offers several benefits, namely the creation of a defined microenvironment which can be designed to modulate stem cell phenotype, protec...
Source: Biotechnology and Bioengineering - December 13, 2012 Category: Biomedical Science Authors: Jenna L. Wilson, Todd C. McDevitt Tags: Review Source Type: research

Whole‐cell‐based CYP153A6‐catalyzed (S)‐limonene hydroxylation efficiency depends on host background and profits from monoterpene uptake via AlkL
In conclusion, undesired perillyl alcohol oxidation was reduced by choosing E. coli's enzymatic background as a reaction environment and co‐expression of the alkL gene in E. coli represents a promising strategy to enhance terpene bioconversion rates. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc.
Source: Biotechnology and Bioengineering - December 13, 2012 Category: Biomedical Science Authors: Sjef Cornelissen, Mattijs K. Julsing, Jan Volmer, Ole Riechert, Andreas Schmid, Bruno Bühler Tags: Article Source Type: research

Functional motor recovery is improved due to local placement of GDNF microspheres after delayed nerve repair
Abstract The majority of bioengineering strategies to promote peripheral nerve regeneration after injury have focused on therapies to bridge large nerve defects while fewer therapies are being developed to treat other nerve injuries, such as nerve transection. We constructed delivery systems using fibrin gels containing either free GDNF or polylactide‐glycolic acid (PLGA) microspheres with GDNF to treat delayed nerve repair, where ELISA verified GDNF release. We determined the formulation of microspheres containing GDNF that optimized nerve regeneration and functional recovery in a rat model of delayed nerve repair. Expe...
Source: Biotechnology and Bioengineering - December 13, 2012 Category: Biomedical Science Authors: Matthew D. Wood, Tessa Gordon, Stephen W. P. Kemp, Edward H. Liu, Howard Kim, Molly S. Shoichet, Gregory H. Borschel Tags: Article Source Type: research

Tumors of the Central Nervous System, Volume 9
Lymphoma, Supratentorial Tumors, Glioneuronal Tumors, Gangliogliomas, Neuroblastoma in Adults, Astrocytomas, Ependymomas, Hemangiomas, and Craniopharyngiomasseries:Tumors of the Central Nervous SystemThe ninth volume in this essential series discusses key advances in our understanding of neoplasms in the human central nervous system. This publication deals with various aspects of nine separate types of brain tumors. With 70 contributors from 17 nations, this edition offers an unrivalled thoroughness and breadth of coverage that includes the very latest research results on the following ...
Source: Springer Biomedical Sciences titles - December 12, 2012 Category: Biomedical Science Tags: Biomedicine (general) Source Type: news

Hot Topics in Infection and Immunity in Children IX
series:Advances in Experimental Medicine and BiologyHot Topics in Infection and Immunity IX
Source: Springer Biomedical Sciences titles - December 12, 2012 Category: Biomedical Science Tags: Immunology Source Type: news

Nature online: December 12, 2012
Rapid regulation of depression-related behaviours by control of midbrain dopamine neurons Dipesh Chaudhury, Jessica J. Walsh, Allyson K. Friedman, Barbara Juarez, Stacy M. Ku, Ja Wook Koo, Deveroux Ferguson, Hsing-Chen Tsai, Lisa Pomeranz, Daniel J. … More »
Source: The Rockefeller University Newswire - December 12, 2012 Category: Biomedical Science Authors: pubaff Tags: Research Update Source Type: news

Geropotency: Increased malignant potential of aging neural progenitors.
Abstract In a syngeneic mouse model increased "age" of neural stem and progenitor cells (NSPCs) prior to transformation pre-determined the malignancy of gliomas regardless of host age. NSPC malignant potential or "geropotency" manifests as increased invasion, genomic instability and tolerance of genotoxic and hypoxic stress in aged transformed and even normal aged NSPCs, phenotypes potentially attributable to differential p53/p16 tumor suppressor function, HIF1 and mTOR activity, H2AX expression and the presence of a subpopulation of aged NSPCs with enhanced cell cycle re-entry. Correlation of age-dependent mali...
Source: Aging - December 12, 2012 Category: Biomedical Science Authors: Mikheev AM, Stoll EA, Ramakrishna R, Mikheeva SA, Horner PJ, Rostomily RC Tags: Aging (Albany NY) Source Type: research

Brain Aging and Therapeutic Interventions
Source: Springer Biomedical Sciences titles - December 11, 2012 Category: Biomedical Science Tags: Biomedicine (general) Source Type: news

Sodium Calcium Exchange: A Growing Spectrum of Pathophysiological Implications
Proceedings of the 6th International Conference on Sodium Calcium Exchangeseries:Advances in Experimental Medicine and BiologyThis book reports the text of the lectures of the 6th International Conference on Sodium Calcium Exchange held in Lacco Ameno in the Island of Ischia in the Gulf of Naples, Italy, from October 1 to October 5, 2011. The present book uncovers the most striking new findings on NCX that emerged since the previous Conference on Sodium Calcium Exchange, such as the structural dissection of the ...
Source: Springer Biomedical Sciences titles - December 11, 2012 Category: Biomedical Science Tags: Neurosciences Source Type: news

The Epidemiology of Aging
Source: Springer Biomedical Sciences titles - December 11, 2012 Category: Biomedical Science Tags: Biomedicine (general) Source Type: news

Novel Immune Potentiators and Delivery Technologies for Next Generation Vaccines
Source: Springer Biomedical Sciences titles - December 11, 2012 Category: Biomedical Science Tags: Immunology Source Type: news

Biophysical and structural investigation of bacterially expressed and engineered CCR5, a G protein-coupled receptor.
Abstract The chemokine receptor CCR5 belongs to the class of G protein-coupled receptors. Besides its role in leukocyte trafficking, it is also the major HIV-1 coreceptor and hence a target for HIV-1 entry inhibitors. Here, we report Escherichia coli expression and a broad range of biophysical studies on E. coli-produced CCR5. After systematic screening and optimization, we obtained 10 mg of purified, detergent-solubilized, folded CCR5 from 1L culture in a triply isotope-labeled ((2)H/(15)N/(13)C) minimal medium. Thus the material is suitable for NMR spectroscopic studies. The expected α-helical secondary structu...
Source: Journal of Bimolecular NMR - December 11, 2012 Category: Biomedical Science Authors: Wiktor M, Morin S, Sass HJ, Kebbel F, Grzesiek S Tags: J Biomol NMR Source Type: research

Characterization of in vivo antioxidant constituents and dual-standard quality assessment of Danhong injection.
Abstract Antioxidants and oxidative stress play a critical role in cardiovascular diseases. Danhong injection (DHI) is a well prescribed cardiovascular medication in China, but its detailed chemical basis and mechanisms of action remain unknown. To prove the antioxidant activity of DHI, its free radical scavenging capacity (RSC) was assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) spectrophotometric assay. The 50% radical scavenging activity value was 1:129.2 mL/mL, against 0.95 m m DPPH. To further identify the antioxidant compounds, modified thin-layer chromatography combined with DPPH bioautography assay was...
Source: Biomedical Chromatography : BMC - December 11, 2012 Category: Biomedical Science Authors: Liu HT, Wang YF, Olaleye O, Zhu Y, Gao XM, Kang LY, Zhao T Tags: Biomed Chromatogr Source Type: research

Embryonic stem cells and inducible pluripotent stem cells: two faces of the same coin?
Abstract Embryonic stem cells (ESCs) are derived from the inner cell mass of the blastocysts and are characterized by the ability to renew themselves (self-renewal) and the capability to generate all the cells within the human body. In contrast, inducible pluripotent stem cells (iPSCs) are generated by transfection of four transcription factors in somatic cells. Like embryonic stem cells, they are able to self-renew and differentiate. Because of these features, both ESCs and iPSCs, are under intense clinical investigation for cell-based therapy. In this review, we revisit stem cell biology and add a new layer of co...
Source: Aging - December 11, 2012 Category: Biomedical Science Authors: Romeo F, Costanzo F, Agostini M Tags: Aging (Albany NY) Source Type: research

p42.3 gene expression in gastric cancer cell and its protein regulatory network analysis
Conclusion: It is of vital significance for mechanism research to find out the action pathway of p42.3 in protein regulatory network, since p42.3 protein plays an important role in the generation and development of GC.
Source: Theoretical Biology and Medical Modelling - December 11, 2012 Category: Biomedical Science Authors: Jianhua ZhangChunlei LuZhigang ShangRui XingLi ShiYouyong Lv Source Type: research

Young Investigator Award winners announced by Anatomy Society
(American Association of Anatomists) The American Association of Anatomist's Young Investigator Awards combine three long-standing AAA awards -- Bensley, Herrick, and Mossman -- with the Morphological Sciences Award, all recognizing investigators in the early stages of their careers who have made important contributions to biomedical science through their research in cell/molecular biology, developmental biology, comparative neuroanatomy, or the morphological sciences.
Source: EurekAlert! - Social and Behavioral Science - December 11, 2012 Category: Global & Universal Source Type: news

Instructions for Authors Pages 2-5
Source: Metabolism - Clinical and Experimental - December 10, 2012 Category: Biomedical Science Tags: Frontmatter Source Type: research

Editorial Board
Source: Metabolism - Clinical and Experimental - December 10, 2012 Category: Biomedical Science Tags: Frontmatter Source Type: research

Table of Contents
Source: Metabolism - Clinical and Experimental - December 10, 2012 Category: Biomedical Science Tags: Frontmatter Source Type: research

Instructions for Authors Page 1
Source: Metabolism - Clinical and Experimental - December 10, 2012 Category: Biomedical Science Source Type: research