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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 36.

Computational evaluation of the thrombogenic potential of a hollow-fiber oxygenator with integrated heat exchanger during extracorporeal circulation.
This study extends the use of computational fluid dynamics simulations to guide the hemodynamic design optimization of oxygenators and evaluate their thrombogenic potential during ECC. The computational analysis accounted for both macro- (i.e., vortex formation) and micro-scale (i.e., flow-induced platelet activation) phenomena affecting the performances of a hollow-fiber membrane oxygenator with integrated heat exchanger. A multiscale Lagrangian approach was adopted to infer the trajectory and loading history experienced by platelet-like particles in the entire device and in a repetitive subunit of the fiber bundles. The ...
Source: Biomechanics and Modeling in Mechanobiology - October 6, 2012 Category: Biomedical Science Authors: Pelosi A, Sheriff J, Stevanella M, Fiore GB, Bluestein D, Redaelli A Tags: Biomech Model Mechanobiol Source Type: research

endoCANNABINOIDS
Actions at Non-CB1/CB2 Cannabinoid Receptorsseries:The ReceptorsThe cloning of two G protein-coupled cannabinoid receptors, termed CB1 and CB2, in the early 1990s has stimulated and facilitated research conducted on the physiological function of cannabinoid actions in the brain and throughout the body. In the twenty years since the identification of these two receptors, endogenous ligands (endocannabinoids) for these receptors have been identified, their ...
Source: Springer Biomedical Sciences titles - October 5, 2012 Category: Biomedical Science Tags: Neurosciences Source Type: news

In vivo cleavage of transgene donors promotes nuclease‐mediated targeted integration
Abstract Targeted DNA integration is commonly used to eliminate position effects on transgene expression. Integration can be targeted to specific sites in the genome via both homology‐based and homology‐independent processes. Both pathways start the integration process with a site‐specific break in the chromosome, typically from a zinc‐finger nuclease (ZFN). We previously described an efficient homology‐independent targeted integration technique that captures short (<100 bp) pieces of DNA at chromosomal breaks created by ZFNs. We show here that inclusion of a nuclease target site on the donor plasmid followed ...
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Sandra Cristea, Yev Freyvert, Yolanda Santiago, Michael C. Holmes, Fyodor D. Urnov, Philip D. Gregory, Gregory J. Cost Tags: Article Source Type: research

JIMD Reports - Case and Research Reports, 2012/5
series:JIMD ReportsJIMD Reports publishes case and short research reports in the area of inherited metabolic disorders. Case reports highlight some unusual or previously unrecorded feature relevant to the disorder, or serve as an important reminder of clinical or biochemical features of a Mendelian disorder.
Source: Springer Biomedical Sciences titles - October 5, 2012 Category: Biomedical Science Tags: Human Genetics Source Type: news

Using Model Organisms to Study Health and Disease
Source: NIGMS - What's New - October 5, 2012 Category: Biomedical Science Tags: N/A Source Type: news

Ducord, HPC Cord Blood
Source: What's New at CBER - October 5, 2012 Category: Biomedical Science Source Type: news

A potential role of the GRO-α/CXCR2 system in Sjögren’s syndrome: regulatory effects of pro-inflammatory cytokines
Abstract Chemokines, small pro-inflammatory cytokines, are involved in migration of inflammatory cells in inflamed tissues and recent studies established their role in angiogenesis, hematopoiesis, cancer and autoimmune conditions. Growth related oncogene-alpha (GRO-α), a member of the CXC chemokine family, and its receptor CXCR2 are involved in the inflammatory processes. Since there is no previous report that supports a possible role of GRO-α/CXCR2 receptor complex during inflammation and neovascularization existing in the autoimmune disease Sjögren’s syndrome (SS), in this study, we examined CXCR2 and...
Source: Histochemistry and Cell Biology - October 5, 2012 Category: Biomedical Science Tags: Histochemistry and Cell Biology Source Type: research

The successful endoscopic hemostasis factors in bleeding from advanced gastric cancer
Conclusions We determined that small bleeding lesions (<2 cm) and exposed vessels in the bleeding site with gastric cancer indicated that endoscopic hemostasis would be an effective hemostatic modality to choose. Particularly, in the opposite condition, the presence of large bleeding lesions (>2 cm) and non-exposed vessel bleeding with a tumor, endoscopic hemostasis failure is predicted and TAE could be recommended. Content Type Journal ArticleCategory Original ArticlePages 1-7DOI 10.1007/s10120-012-0200-3Authors Kang Hun Koh, Department of Internal Medicine, Research Institute for ...
Source: Gastric Cancer - October 5, 2012 Category: Gastroenterology Tags: Gastric Cancer Source Type: research

l-Arginine enriched biscuits improve endothelial function and glucose metabolism: A pilot study in healthy subjects and a cross-over study in subjects with impaired glucose tolerance and metabolic syndrome
Conclusions: l-Arginine-enriched biscuits with low sugar and protein content enhance endothelial function and improve glucose metabolism, insulin sensitivity and insulin secretion in subjects with IGT and MS.
Source: Metabolism - Clinical and Experimental - October 5, 2012 Category: Biomedical Science Authors: Lucilla D. Monti, Maria C. Casiraghi, Emanuela Setola, Elena Galluccio, Maria A. Pagani, Lucio Quaglia, Emanuele Bosi, PierMarco Piatti Tags: Clinical Science Source Type: research

Brain-derived neurotrophic factor and its relation to leptin in obese children before and after weight loss
Abstract: Objective: Brain-derived neurotrophic factor (BDNF) is a regulator of energy homeostasis and food intake through hypothalamic signaling. Currently, data regarding BDNF in children with obesity are lacking. We evaluated serum BDNF concentrations in obese children, both before and after lifestyle intervention, in reference to those of lean children.Methods: A total of 90 (24 normal weight; 66 obese) children were studied utilizing a cross-sectional clinical outpatient study design. In addition, longitudinal data analysis was performed in 30 obese children participating in a lifestyle intervention for one year.Resul...
Source: Metabolism - Clinical and Experimental - October 5, 2012 Category: Biomedical Science Authors: Christian L. Roth, Clinton Elfers, Ursel Gebhardt, Hermann L. Müller, Thomas Reinehr Tags: Clinical Science Source Type: research

The effect of osteocyte apoptosis on signalling in the osteocyte and bone lining cell network: a computer simulation
Abstract: Osteocytes play a critical role in the regulation of bone remodelling by translating strain due to mechanical loading into biochemical signals transmitted through the interconnecting lacuno-canalicular network to bone lining cells (BLCs) on the bone surface. This work aims to examine the effects of disruption of that intercellular communication by simulation of osteocyte apoptosis in the bone matrix. A model of a uniformly distributed osteocyte network has been developed that simulates the signalling through the network to the BLCs based on strain level. Bi-directional and asymmetric communication between neighbo...
Source: Journal of Biomechanics - October 5, 2012 Category: Biomedical Science Authors: Masoumeh Jahani, Paul G. Genever, Ronald J. Patton, Fadi Ahwal, Michael J. Fagan Tags: Papers Source Type: research

Helical flows and asymmetry of blood jet in dilated ascending aorta with normally functioning bicuspid valve.
This study seeks to elucidate the connections between jet asymmetry and helical flow in patients with normally functioning BAV and dilated ascending aorta. We performed a computational parametric study by varying, for a patient-specific geometry, the valve area and the flow rate entering the aorta and drawing also a tricuspid valve (TAV). We considered also phase-contrast magnetic resonance imaging of four BAV and TAV patients. Measurement of normalized flow asymmetry index, systolic WSS and of a new index (positive helix fraction, PHF) quantifying the presence of a single a single helical flow were performed. In our compu...
Source: Biomechanics and Modeling in Mechanobiology - October 5, 2012 Category: Biomedical Science Authors: Faggiano E, Antiga L, Puppini G, Quarteroni A, Luciani GB, Vergara C Tags: Biomech Model Mechanobiol Source Type: research

Identification and profiling of targeted oxidized linoleic acid metabolites in rat plasma by quadrupole time-of-flight mass spectrometry.
This article is a U.S. Government work and is in the public domain in the USA. PMID: 23037960 [PubMed - as supplied by publisher]
Source: Biomedical Chromatography : BMC - October 5, 2012 Category: Biomedical Science Authors: Yuan ZX, Rapoport SI, Soldin SJ, Remaley AT, Taha AY, Kellom M, Gu J, Sampson M, Ramsden CE Tags: Biomed Chromatogr Source Type: research

Quantitative determination of rupestonic acid in rat plasma by high-performance liquid chromatography-tandem mass spectrometry and its application in a pharmacokinetic study.
Abstract A sensitive and specific high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was developed and validated for determination of rupestonic acid in rat plasma. Protein precipitation method was used to extract rupestonic acid and the internal standard (IS) warfarin sodium from rats plasma. The chromatographic separation was performed on an Agela Venusil XBP Phenyl column with an isocratic mobile phase consisting of methanol-0.1% formic acid in water (40:60, v/v), pumped at 0.4 mL/min. Rupestonic acid and the internal standard (IS) warfarin sodium were...
Source: Biomedical Chromatography : BMC - October 5, 2012 Category: Biomedical Science Authors: Gu Z, He J, Yang X, Sha X Tags: Biomed Chromatogr Source Type: research

Accurate retention time determination of co‐eluting proteins in analytical chromatography by means of spectral data
Abstract Chromatography is the method of choice for the separation of proteins, at both analytical and preparative scale. Orthogonal purification strategies for industrial use can easily be implemented by combining different modes of adsorption. Nevertheless, with flexibility comes the freedom of choice and optimal conditions for consecutive steps need to be identified in a robust and reproducible fashion. One way to address this issue is the use of mathematical models that allow for an in silico process optimization. Although this has been shown to work, model parameter estimation for complex feedstocks becomes the bottle...
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Florian Dismer, Sigrid Hansen, Stefan Alexander Oelmeier, Jürgen Hubbuch Tags: Article Source Type: research

Development of thermodynamic optimum searching (TOS) to improve the prediction accuracy of flux balance analysis
Abstract Flux balance analysis (FBA) has been widely used in calculating steady‐state flux distributions that provide important information for metabolic engineering. Several thermodynamics‐based methods, e.g. quantitative assignment of reaction directionality and energy balance analysis have been developed to improve the prediction accuracy of FBA. However, these methods can only generate a thermodynamically feasible range, rather than the most thermodynamically favorable solution. We therefore developed a novel optimization method termed as Thermodynamic Optimum Searching (TOS) to calculate the thermodynamically opti...
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Yan Zhu, Jiangning Song, Zixiang Xu, Jibin Sun, Yanping Zhang, Yin Li, Yanhe Ma Tags: Article Source Type: research

Recombinant tagging system using ribosomal frameshifting to monitor protein expression
Abstract For rapid and accurate quantitation of recombinant proteins during expression and after purification, we introduce a new tagging strategy that expresses both target proteins and limitedly tagged target proteins together in a single cell at a constant ratio by utilizing cis‐elements of programmed ‐1 ribosomal frameshifting (‐1RFS) as an embedded device. ‐1RFS is an alternative reading mechanism that effectively controls protein expression by many viruses. When a target gene is fused to the enhanced green fluorescent protein (EGFP) gene with a ‐1RFS element implanted between them, the unfused target and th...
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Se Jong Han, Sayeon Cho, Ky Lowehhaupt, So‐Young Park, Sang Jun Sim, Yang‐Gyun Kim Tags: Article Source Type: research

Rapid determination of sugar content in biomass hydrolysates using nuclear magnetic resonance spectroscopy
Abstract Large populations of potential cellulosic biomass feedstocks are currently being screened for fuel and chemical applications. The monomeric sugar content, released through hydrolysis, is of particular importance and is currently measured with time‐consuming HPLC methods. A method for sugar detection is presented here that employs 1H NMR spectra regressed against primary HPLC sugar concentration data to build Partial Least Squares models. The PLS2 model is able to predict concentrations of both major sugar components, like glucose and xylose, and minor sugars, such as arabinose and mannose, in biomass hydrolysate...
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Erica Gjersing, Renee M. Happs, Robert W. Sykes, Crissa Doeppke, Mark F. Davis Tags: Article Source Type: research

Development of a platform process for adenovirus purification that removes human SET and nucleolin and provides high purity vector for gene delivery
This study describes our approach to develop a scalable, manufacturable robust process for an Adenovirus (Ad) gene therapy vector. A number of analytical tools were developed to guide the purification design. During process development, two human proteins, SET and nucleolin, were identified in viral preparations. To our knowledge, this is the first time that SET and nucleolin have been described in Ad. In this report we detail a process for their removal and the robust removal of all process, product and host cell impurities. Biotechnol. Bioeng. © 2012 Wiley Periodicals, Inc.
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Frank Riske, Nicole Berard, Karen Albee, Peng Pan, Mike Henderson, Kris Adams, Simon Godwin, Sherri Spear Tags: Article Source Type: research

Reconstitution of cellulose and lignin after [C2mim][OAc] pretreatment and its relation to enzymatic hydrolysis
In this study, reconstitution of cellulose and lignin after 1‐ethyl‐3‐methylimidazolium acetate ([C2mim][OAc]) pretreatment was proposed as a new method to study their effects on enzymatic digestibility. Different mechanisms of lignin content for reduction of cellulose hydrolysis were found between the proposed method and the traditional method (mixing of cellulose and lignin). The results indicated that a slight change of the crystallinity of the reconstituted materials may play a minor role in the change of enzyme efficiency. Additionally, the present study suggested that the lignin content does not significantly a...
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Tong‐Qi Yuan, Wei Wang, Li‐Ming Zhang, Feng Xu, Run‐Cang Sun Tags: Article Source Type: research

Carbohydrate derived pseudo‐lignin can retard cellulose biological conversion
Abstract Dilute acid as well as water only (hydrothermal) pretreatments often lead to a significant hemicellulose loss to soluble furans and insoluble degradation products, collectively termed as chars and/or pseudo‐lignin. In order to understand the factors contributing to reducing sugar yields from pretreated biomass and the possible influence of hemicellulose derived pseudo‐lignin on cellulose conversion at the moderate to low enzyme loadings necessary for favorable economics, dilute acid pretreatment of Avicel cellulose alone and mixed with beechwood xylan or xylose was performed at various severities. Following pr...
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Rajeev Kumar, Fan Hu, Poulomi Sannigrahi, Seokwon Jung, Arthur J. Ragauskas, Charles E. Wyman Tags: Article Source Type: research

Stepwise reduction of the culture redoxpotential allows the analysis of microaerobic metabolism and photosynthetic membrane synthesis in Rhodospirillum rubrum
Abstract Bacterial growth under oxygen‐limited (microaerobic) conditions is often accompanied by phenomena of great interest for fundamental research and industrial application. The microaerobic lifestyle of anoxygenic photosynthetic bacteria like Rhodospirillum rubrum harbors such a phenomenon, as it allows the formation of photosynthetic membranes and related interesting products without light. However, due to the technical difficulties in process control of microaerobic cultivations and the limited sensitivity of available oxygen sensors, the analysis of microaerobic growth and physiology is still underrepresented in ...
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Lisa Carius, Oliver Hädicke, Hartmut Grammel Tags: Article Source Type: research

Ultrahigh frequency lensless ultrasonic transducers for acoustic tweezers application
Abstract Similar to optical tweezers, a tightly focused ultrasound microbeam is needed to manipulate microparticles in acoustic tweezers. The development of highly sensitive ultrahigh frequency ultrasonic transducers is crucial for trapping particles or cells with a size of a few microns. As an extra lens would cause excessive attenuation at ultrahigh frequencies, two types of 200‐MHz lensless transducer design were developed as an ultrasound microbeam device for acoustic tweezers application. Lithium niobate single crystal press‐focused transducer and zinc oxide self‐focused transducer were designed, fabricated and ...
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Kwok Ho Lam, Hsiu‐Sheng Hsu, Ying Li, Changyang Lee, Anderson Lin, Qifa Zhou, EunSok Kim, Kirk Koping Shung Tags: Article Source Type: research

Enhanced cell viability via strain stimulus and fluid flow in magnetically actuated scaffolds
Abstract A novel magnetically actuated scaffold was used to explore the effects of strain stimulus on the proliferation and spatial distribution of smooth muscle cells and improve cell viability in the scaffold interior by pumping nutrients throughout the structure. Magnetically actuable scaffolds were fabricated in a tube shape by winding electrospun sheets of a biodegradable polymer modified with magnetic Fe2O3 nanoparticles. Prior to rolling, the sheets were seeded with smooth muscle cells and wound into tubes with diameter 5.2 mm and wall thickness 0.2 mm. The tubular scaffolds were actuated by a magnetic field to ...
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Julia Mack, Abigail Corrin, Sergio Lucato, James Dunn, Ben Wu, Brian Cox Tags: Article Source Type: research

In vitro selection of sialic acid specific RNA aptamer and its application to the rapid sensing of sialic acid modified sugars
Abstract Sialic acids are located on the terminal positions of glycan on a cell surface, which play important role in the spread and metastasis of cancer cells and infection of pathogen. For their detection and diagnosis, the finding of sialic acid specific ligand is an essential prerequisite. Here, RNA aptamer for N‐acetylneuraminic acid (Neu5Ac), a representative of sialic acids, with the high affinity of 1.35 nM and the selectivity was screened by in vitro selection method. The strong binding of the screened aptamer was enough to protect the hydrolysis of Neu5Ac by neuraminidase with the stoichiometry of 1:1 molar rat...
Source: Biotechnology and Bioengineering - October 5, 2012 Category: Biomedical Science Authors: Suhyung Cho, Bo‐Rahm Lee, Byung‐Kwan Cho, June‐Hyung Kim, Byung‐Gee Kim Tags: Article Source Type: research

Novel Antischizophrenia Treatments
series:Handbook of Experimental Pharmacology.
Source: Springer Biomedical Sciences titles - October 4, 2012 Category: Biomedical Science Tags: Pharmacology / Toxicology Source Type: news

Risk factors for the exacerbation of esophageal varices or portosystemic encephalopathy after sustained virological response with IFN therapy for HCV-related compensated cirrhosis
Conclusions Our findings suggest that the existence of radical portosystemic collateral vessels at successful HCV eradication increases the risk of the exacerbation of EVs and the incidence of portosystemic encephalopathy in patients with HCV-related cirrhosis. Content Type Journal ArticleCategory Original Article—Liver, Pancreas, and Biliary TractPages 1-9DOI 10.1007/s00535-012-0679-8Authors Yuko Nagaoki, Programs for Applied Biomedicine, Division of Frontier Medical Science, Department of Medicine and Molecular Science, Graduate School of Biomedical Science, Hiroshima University, 1-2-3 Kasumi, Mi...
Source: Journal of Gastroenterology - October 4, 2012 Category: Gastroenterology Tags: Journal of Gastroenterology Source Type: research

Fraud in biomed research higher than thought
Are biomedical scientists becoming more dishonest? Research retractions for fraud or suspected fraud have increased tenfold since 1975
Source: New Scientist - Health - October 4, 2012 Category: Consumer Health News Source Type: research

Spatially controlled photothermal heating of bladder tissue through single-walled carbon nanohorns delivered with a fiberoptic microneedle device
This study evaluates the utilization of sharp, silica, fiberoptic microneedle devices (FMDs) to deliver single-walled carbon nanohorns (SWNHs) serving as exogenous chromophores in conjunction with a 1,064-nm laser to amplify thermal treatment doses in a spatially controlled manner. Experiments were conducted to determine the lateral and depth dispersal of SWNHs in aqueous solution (0.05 mg/mL) infused through FMDs into the wall of healthy, inflated, ex vivo porcine bladders. SWNH-perfused bladder regions were irradiated with a free-space, CW, 1,064-nm laser in order to determine the SWNH efficacy as exogenous chro...
Source: Lasers in Medical Science - October 4, 2012 Category: Laser Surgery Tags: Lasers in Medical Science Source Type: research

The application of 120-W high-performance system GreenLight laser vaporization of the prostate in high-risk patients
Abstract The purpose of this study is to evaluate the safety and efficacy of 120-W potassium titanyl phosphate (KTP) laser vaporization in patients with benign prostatic hyperplasia (BPH) who also had cardiopulmonary diseases who were taking long-term anticoagulants and were at high risk of bleeding complications. The prospective study included 188 patients with severe lower urinary tract symptoms who underwent 120-W KTP laser vaporization of the prostate. All patients were at high cardiopulmonary risk, having presented with an American Society of Anesthesiology score of 3 or greater. Of those, 45 patients w...
Source: Lasers in Medical Science - October 4, 2012 Category: Laser Surgery Tags: Lasers in Medical Science Source Type: research

Characterization of the erythropoietin/erythropoietin receptor axis in a rat model of liver damage and cholangiocarcinoma development
This study suggests that the autocrine and paracrine release of endogenous EPO in the microenvironment may contribute to the development and maintenance of the CC possibly in cooperation with other signaling pathways. Content Type Journal ArticleCategory Original PaperPages 1-13DOI 10.1007/s00418-012-1037-xAuthors Federico Moriconi, Department of Gastroenterology and Endocrinology, Center of Internal Medicine, University of Göttingen, Robert Koch Straße 40, 37075 Göttingen, GermanyPierluigi Ramadori, Department of Cell Physiology and Metabolism, University Medical Centre of Geneva, Geneva, SwitzerlandFrank C. S...
Source: Histochemistry and Cell Biology - October 4, 2012 Category: Biomedical Science Tags: Histochemistry and Cell Biology Source Type: research

Fast rearrangement of the neuronal growth cone’s actin cytoskeleton following VEGF stimulation
Abstract The neuronal growth cone plays a crucial role in the development of the nervous system. This highly motile structure leads the axon to its final destination by translating guidance cues into cytoskeletal rearrangements. Recently, vascular endothelial growth factor (VEGF), which is essential for angiogenesis and vascular sprouting, has been found to exert a trophic activity also on neurons, leading to an increased axonal outgrowth, similar to the well-known nerve growth factor (NGF). The neurotrophic properties of VEGF are likely to be promoted via the VEGF receptor 2 (VEGFR-2) and neuropilin-1 (NRP-...
Source: Histochemistry and Cell Biology - October 4, 2012 Category: Biomedical Science Tags: Histochemistry and Cell Biology Source Type: research

Systematic nonlinear relations between joint mechanics and the neural reflex response with changes in stretch amplitude at the wrist
Abstract: The effects on both joint mechanics and the neural stretch reflex of changes in amplitude of stretch ranging from within the region of short range stiffness up to 3% of resting muscle length were quantified. The level of activation of the primary agonist was held constant but there was a small but systematic increase in activation of the other flexor and extensor muscles as stretch amplitude increased. The patterns of change with stretch amplitude in both the joint mechanics and stretch reflex properties were non-linear but systematic and were well described by power relationships that accounted for high proporti...
Source: Journal of Biomechanics - October 4, 2012 Category: Biomedical Science Authors: Mark Halaki, Nicholas O'Dwyer, Ian Cathers, Stephane Heritier Tags: Papers Source Type: research

A lattice-based approach to model distraction osteogenesis
Abstract: Distraction osteogenesis is a well-known technique in which new bone tissue is created when a distraction displacement is applied through an external frame. This orthopedic process is nowadays focus of intense research, both experimentally and numerically, as there are still many aspects not well understood. The aim of this study is to simulate bone distraction by means of a combined discrete-continuum approach based on a lattice formulation. Existing computational models simulate the main processes of distraction osteogenesis from a continuum perspective, considering as state variables the population of cells an...
Source: Journal of Biomechanics - October 4, 2012 Category: Biomedical Science Authors: E. Reina-Romo, M.J. Gómez-Benito, J. Domínguez, J.M. García-Aznar Tags: Papers Source Type: research

Experimental model of tooth movement in mice: A standardized protocol for studying bone remodeling under compression and tensile strains
Abstract: During orthodontic tooth movement (OTM), alveolar bone is resorbed by osteoclasts in compression sites (CS) and is deposited by osteoblasts in tension sites (TS). The aim of this study was to develop a standardized OTM protocol in mice and to investigate the expression of bone resorption and deposition markers in CS and TS. An orthodontic appliance was placed in C57BL6/J mice. To define the ideal orthodontic force, the molars of the mice were subjected to forces of 0.1N, 0.25N, 0.35N and 0.5N. The expression of mediators that are involved in bone remodeling at CS and TS was analyzed using a Real-Time PCR. The dat...
Source: Journal of Biomechanics - October 4, 2012 Category: Biomedical Science Authors: Silvana Rodrigues de Albuquerque Taddei, Adriana Pedrosa Moura, Ildeu Andrade, Gustavo Pompermaier Garlet, Thiago Pompermaier Garlet, Mauro Martins Teixeira, Tarcília Aparecida da Silva Tags: Papers Source Type: research

Imatinib assay by high-performance liquid chromatography in tandem mass spectrometry with solid-phase extraction in human plasma.
Abstract We have developed a method of liquid chromatography in tandem with mass spectrometry to monitor therapeutic levels of imatinib in plasma, a selective inhibitor of protein tyrosine kinase. After solid-phase extraction of plasma samples, imatinib and its internal standard, imatinib-D8, were eluted with Zorbax SB-C(18) at 60 °C, under isocratic conditions through a mobile phase consisting of 4 m m ammonium formate, pH: 3.2 (solution A) and acetonitrile solution B. The flow rate was 0.8 mL/min with 55% solution A + 45% solution B. Imatinib was detected and quantified by mass spectrometry with electr...
Source: Biomedical Chromatography : BMC - October 4, 2012 Category: Biomedical Science Authors: Moreno JM, Wojnicz A, Steegman JL, Cano-Abad MF, Ruiz-Nuño A Tags: Biomed Chromatogr Source Type: research

Trans-Golgi proteins participate in the control of lipid droplet and chylomicron formation.
Abstract Lipid droplets carrying triglycerides and cholesteryl esters are emerging as dynamic cellular organelles that are generated in nearly every cell. They play a key role in lipid and membrane homeostasis. Abnormal lipid droplet dynamics are associated with the pathophysiology of many metabolic diseases, such as obesity, diabetes, atherosclerosis, fatty liver, and even cancer. Chylomicrons, stable droplets also consisting of triglycerides and cholesterol are generated in the intestinal epithelium to transport exogenous (dietary) lipids after meals from the small intestine to tissues for degradation. Defective ...
Source: Bioscience Reports - October 4, 2012 Category: Biomedical Science Authors: Hesse D, Jaschke A, Chung B, Schürmann A Tags: Biosci Rep Source Type: research

Improving promiscuous mammalian cell entry by the baculovirus AcMNPV.
Abstract The insect baculovirus Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) enters many mammalian cell lines, prompting its application as a general eukaryotic gene delivery agent, but the basis of entry is poorly understood. For adherent mammalian cells we show that entry is favoured by low pH and increasing the available cell surface area through transient release from the substratum. Low pH also stimulated baculovirus entry into mammalian cells grown in suspension which, optimally, could reach 90% of the transduced population. The basic loop, residues 268-281, of the viral surface glycopr...
Source: Bioscience Reports - October 4, 2012 Category: Biomedical Science Authors: O'Flynn NM, Patel A, Kadlec J, Jones IM Tags: Biosci Rep Source Type: research

Protein labeling with the labeling precursor [18F]SiFA-SH for positron emission tomography
Nature Protocols 7, 1964 (2012). doi:10.1038/nprot.2012.111 Authors: Björn Wängler, Alexey P Kostikov, Sabrina Niedermoser, Joshua Chin, Katy Orchovski, Esther Schirrmacher, Liuba Iovkova-Berends, Klaus Jurkschat, Carmen Wängler & Ralf Schirrmacher Proteins previously derivatized with the cross-coupling reagent sulfo-SMCC (4-(N-maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxy-succinimide ester sodium salt) can be easily labeled in high radiochemical yields with the silicon-fluoride acceptor (SiFA) reagent [18F]SiFA-SH, obtained via isotopic exchange, by thiol-maleimide coupling
Source: Nature Protocols - October 4, 2012 Category: Biomedical Science Authors: Björn WänglerAlexey P KostikovSabrina NiedermoserJoshua ChinKaty OrchovskiEsther SchirrmacherLiuba Iovkova-BerendsKlaus JurkschatCarmen WänglerRalf Schirrmacher Tags: Protocol Source Type: research

Synthesis of [18F]SiFB: a prosthetic group for direct protein radiolabeling for application in positron emission tomography
Nature Protocols 7, 1956 (2012). doi:10.1038/nprot.2012.110 Authors: Alexey P Kostikov, Joshua Chin, Katy Orchovski, Esther Schirrmacher, Sabrina Niedermoser, Klaus Jurkschat, Liuba Iovkova-Berends, Carmen Wängler, Björn Wängler & Ralf Schirrmacher N-Succinimidyl 3-(di-tert-butyl[18F]fluorosilyl)benzoate ([18F]SiFB) is a highly reactive prosthetic group for radiolabeling of proteins for use in positron emission tomography (PET). It is similar to N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB), the 'gold-standard' prosthetic group
Source: Nature Protocols - October 4, 2012 Category: Biomedical Science Authors: Alexey P KostikovJoshua ChinKaty OrchovskiEsther SchirrmacherSabrina NiedermoserKlaus JurkschatLiuba Iovkova-BerendsCarmen WänglerBjörn WänglerRalf Schirrmacher Tags: Protocol Source Type: research

One-step 18F-labeling of peptides for positron emission tomography imaging using the SiFA methodology
Nature Protocols 7, 1946 (2012). doi:10.1038/nprot.2012.109 Authors: Carmen Wängler, Sabrina Niedermoser, Joshua Chin, Katy Orchovski, Esther Schirrmacher, Klaus Jurkschat, Liuba Iovkova-Berends, Alexey P Kostikov, Ralf Schirrmacher & Björn Wängler Here we present a procedure to label peptides with the positron-emitting radioisotope fluorine-18 (18F) using the silicon-fluoride acceptor (SiFA) labeling methodology. Positron emission tomography (PET) has gained high importance in noninvasive imaging of various diseases over the past decades, and thus new specific
Source: Nature Protocols - October 4, 2012 Category: Biomedical Science Authors: Carmen WänglerSabrina NiedermoserJoshua ChinKaty OrchovskiEsther SchirrmacherKlaus JurkschatLiuba Iovkova-BerendsAlexey P KostikovRalf SchirrmacherBjörn Wängler Tags: Protocol Source Type: research

Using multiplex single-base extension typing to screen for mutants defective in RNA editing
Nature Protocols 7, 1931 (2012). doi:10.1038/nprot.2012.117 Authors: Mizuki Takenaka & Axel Brennicke RNA editing is an RNA maturation process that changes the nucleotide present at particular positions (editing sites) in specific RNAs; in plant organelles, the most common nucleotide change is from cytidine (C) to uridine (U). In a mutant suspected of affecting RNA editing,
Source: Nature Protocols - October 4, 2012 Category: Biomedical Science Authors: Mizuki TakenakaAxel Brennicke Tags: Protocol Source Type: research

TRP Channels in Drug Discovery
Volume IIseries:Methods in Pharmacology and ToxicologyRecent findings have implied a distinct therapeutic potential for drugs targeting Transient Receptor Potential (TRP) channels in a wide variety of diseases, many with no existing satisfactory treatment options. Thus, the TRP superfamily of ion channels has attracted a great deal of well-deserved attention. TRP Channels in Drug Discovery provides a thorough collection of the most up-to-date ...
Source: Springer Biomedical Sciences titles - October 3, 2012 Category: Biomedical Science Tags: Pharmacology / Toxicology Source Type: news

Sex and Gender Differences in Pharmacology
series:Handbook of Experimental PharmacologyThis is the very first book to deal with sex and gender differences in drug therapy - an increasingly recognized medical need. It starts with an overview on S/G in clinical syndromes and a documentation of the medical and socioeconomic damage caused by gender specific adverse drug effects. Part I covers S/G differences in pharmacokinetics. Researchers will be satisfied by the detailed ...
Source: Springer Biomedical Sciences titles - October 3, 2012 Category: Biomedical Science Tags: Pharmacology / Toxicology Source Type: news

Plasmonic Tuning of Photoluminescence from Semiconducting Quantum Dot Assemblies
We report tuning of photoluminescence enhancement and quenching from closed packed monolayers of cadmium selenide quantum dots doped with gold nanoparticles. Plasmon-mediated control of the emission intensity from the monolayers is achieved by varying the size and packing density of the quantum dots as well as the doping concentration of gold nanoparticles. We observe a unique packing density dependent crossover from enhancement to quenching and vice versa for fixed size of quantum dots and doping concentration of gold nanoparticles. We suggest that this behavior is indicative of a crossover from single particle to co...
Source: Plasmonics - October 3, 2012 Category: Biomedical Science Tags: Plasmonics Source Type: research

Expression and localization of nodal in bovine oviduct and uterus during different functional stages of oestrus cycle and pregnancy
Abstract Members of TGF-β superfamily play a major role in the endometrial changes involved in the establishment and maintenance of pregnancy. Their deregulated expression and action could lead to absolute or partial failure of embryo implantation. Nonetheless, the precise function and mechanism of many of these cytokines remain unclear. Nodal, a transforming growth factor beta (TGF-β) superfamily member, was characterized in the human and rodent uterus and implicated in the tissue remodeling events during menstruation and embryo implantation. In order to study its possible role in the cattle reproductive ...
Source: Histochemistry and Cell Biology - October 3, 2012 Category: Biomedical Science Tags: Histochemistry and Cell Biology Source Type: research

Allogeneic hematopoietic stem cell transplantation for patients with mildly reduced renal function as defined based on creatinine clearance before transplantation
In conclusion, NRM may be associated with mildly reduced renal function before allogeneic HSCT, which cannot be detected by measurement of the serum creatinine level alone. Content Type Journal ArticleCategory Original ArticlePages 1-6DOI 10.1007/s00277-012-1584-1Authors Kumi Oshima, Division of Hematology, Saitama Medical Center, Jichi Medical University, 1-847, Amanuma-cho, Omiya-ku, Saitama-shi, Saitama 330-8503, JapanYoshinobu Kanda, Division of Hematology, Saitama Medical Center, Jichi Medical University, 1-847, Amanuma-cho, Omiya-ku, Saitama-shi, Saitama 330-8503, JapanYasuhito Nanya, Department of Hematology...
Source: Annals of Hematology - October 3, 2012 Category: Hematology Tags: Annals of Hematology Source Type: research

Erratum to: Adenosine A2A Receptor and TNF-α Regulate the Circadian Machinery of the Human Monocytic THP-1 Cells
Content Type Journal ArticleCategory ErratumPages 1-1DOI 10.1007/s10753-012-9553-3Authors Miguel Perez-Aso, Division of Translational Medicine, Department of Medicine, New York University School of Medicine, 550 First Avenue, MSB255, New York, NY 10016, USAJessica L. Feig, Division of Translational Medicine, Department of Medicine, New York University School of Medicine, 550 First Avenue, MSB255, New York, NY 10016, USAAránzazu Mediero, Division of Translational Medicine, Department of Medicine, New York University School of Medicine, 550 First Avenue, MSB255, New York, NY 10016, USABruce N. Cronstein, Division of Tra...
Source: Inflammation - October 3, 2012 Category: Biomedical Science Tags: Inflammation Source Type: research

Sex Differences in Prophylaxis and Therapeutic Treatments for Viral Diseases.
Abstract The intensity and prevalence of viral infections are typically higher in males than in females. In contrast, disease outcome can be worse for females. Males and females also differ in their responses to prophylaxis and therapeutic treatments for viral diseases. In response to vaccines against herpes viruses, hepatitis viruses, influenza viruses, and others, females consistently mount higher humoral immune responses and experience more frequent and severe adverse reactions than males. Males and females also differ in the absorption, metabolism, and clearance of antiviral drugs. The pharmacological effects, ...
Source: Herpes - October 3, 2012 Category: Infectious Diseases Authors: Klein SL Tags: Handb Exp Pharmacol Source Type: research