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Biomedical Science

This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 9.

Targeting reduction of proteinuria in glomerulonephritis: Maximizing the antifibrotic effect of valsartan by protecting podocytes
Although angiotensin (Ang) II blockade has become a standard antifibrotic therapy in kidney disease, the therapeutic efficacy of Ang II blockade is yet to be optimized. Considering the prognostic impact of proteinuria reduction, we hypothesized that titration of Ang II blockade for optimal anti-proteinuric effect would improve renoprotection. One day after induction of Thy 1.1 glomeruonephritis, rats were treated with increasing doses of the Ang II receptor blocker valsartan in drinking water. Six days after disease induction, the therapeutic effect on proteinuria, podocyte injury and glomerular fibrosis was evaluated. Inc...
Source: Journal of the Renin-Angiotensin-Aldosterone System : JRAAS - June 9, 2014 Category: Biomedical Science Authors: Gu, C., Zhou, G., Noble, N. A., Border, W. A., Cheung, A. K., Huang, Y. Tags: Articles Source Type: research

The M235T polymorphism in the angiotensinogen gene and heart failure: a meta-analysis
Conclusions: The current meta-analysis suggests that M235T polymorphism might be associated with increased risk of heart failure in Caucasians. (Source: Journal of the Renin-Angiotensin-Aldosterone System : JRAAS)
Source: Journal of the Renin-Angiotensin-Aldosterone System : JRAAS - June 9, 2014 Category: Biomedical Science Authors: Chen, S., Zhang, L., Wang, H.-W., Wang, X.-Y., Li, X.-Q., Zhang, L.-L. Tags: Articles Source Type: research

Effects of antihypertensive therapy on glucose, insulin metabolism, left ventricular diastolic dysfunction and renin system in overweight and obese hypertensives
Conclusions: Optimal target BP was achieved in the group as a whole and in both obese patient groups, while benefits to cardiac structure were of a smaller magnitude. In high-risk, overweight/obese patients with hypertension, traditional therapy provides significantly greater BP- versus aliskiren-lowering throughout the 24-hour dosing interval. Therefore in obese, hypertensive individuals, adequate and similar blood pressure control was achieved with aliskiren; however, the aliskiren group and not the control group was associated with a more favorable GIM profile and led to a significant regression of LVM; overall aliskire...
Source: Journal of the Renin-Angiotensin-Aldosterone System : JRAAS - June 9, 2014 Category: Biomedical Science Authors: De Rosa, M. L., Musella, F., Ilardi, F., D'Amore, C., Luciano, R., Maresca, F. Tags: Articles Source Type: research

FAD binding, cobinamide binding and active site communication in the corrin reductase CobR.
Abstract Adenosylcobalamin, the coenzyme form of vitamin B12, is one Nature's most complex coenzymes whose de novo biogenesis proceeds along either an anerobic or aerobic metabolic pathway. The aerobic synthesis involves reduction of the centrally chelated cobalt metal ion of the corrin ring from Co(II) to Co(I) before adenosylation can take place. A corrin reductase enzyme, CobR, has been identified as the likely agent to catalyse this reduction of the metal ion. Herein, we reveal how Brucella melitensis corrin reductase CobR binds its coenzyme FAD and we also show that the enzyme can bind a corrin substrate consi...
Source: Bioscience Reports - June 9, 2014 Category: Biomedical Science Authors: Lawrence AD, Taylor SL, Scott A, Rowe ML, Johnson CM, Rigby SE, Geeves MA, Pickersgill RW, Howard MJ, Warren MJ Tags: Biosci Rep Source Type: research

Endoplasmic Reticulum Stress Response in Yeast and Humans.
Abstract Stress pathways monitor intracellular systems and deploy a range of regulatory mechanisms in response to stress. One of the best-characterized pathways, the unfolded protein response (UPR), is an intracellular signal transduction pathway that monitors endoplasmic reticulum (ER) homeostasis. Its activation is required to alleviate the effects of ER stress and is highly conserved from yeast to human. Although metazoans have three UPR outputs, yeast cells rely exclusively on the inositol-requiring enzyme-1 (Ire1) pathway, which is conserved in all Eukaryotes. In general, the UPR program activates hundreds of ...
Source: Bioscience Reports - June 9, 2014 Category: Biomedical Science Authors: Wu H, Ng BS, Thibault G Tags: Biosci Rep Source Type: research

n-3 LCPUFA improves cognition: The young, the old and the sick
Abstract: Due to the implication of docosahexaenoic acid (DHA) in neurogenesis, synaptogenesis, neurite outgrowth and to its high incorporation into the brain, this n-3 long chain polyunsaturated fatty acid (LCPUFA) is considered as crucial in the development and maintenance of the learning memory performance throughout life. In the present chapter we aimed at reviewing data investigating the relation between DHA and cognition during the perinatal period, young adult- and adulthood and neurodegenerative diseases such as Alzheimer disease (AD). In Humans, dietary DHA supplementation from the perinatal period to adulthood do...
Source: Prostaglandins, Leukotrienes and Essential Fatty Acids - June 9, 2014 Category: Biomedical Science Authors: C. Joffre, A. Nadjar, M. Lebbadi, F. Calon, S. Laye Tags: Review article Source Type: research

Effect of Hawk-Dove Game on the Dynamics of Two Competing Species.
Abstract Outcomes of interspecific competition, and especially the possibility of coexistence, have been extensively studied in theoretical ecology because of their implications in community assemblages. During the last decades, the influence of different time scales through the local/regional dynamics of animal communities has received an increasing attention. Nevertheless, different time scales involved in interspecific competition can result form other processes than spatial dynamics. Here, we envision and analyze a new theoretical framework that couples a game theory approach for competition with a demographic ...
Source: Acta Biotheoretica - June 8, 2014 Category: Biomedical Science Authors: Moussaoui A, Auger P, Roche B Tags: Acta Biotheor Source Type: research

Rapid determination of cellulose
Abstract The cellulose analysis results of 4 feedstocks and Avicel obtained by a one‐step/two‐step hydrolysis method were compared to the conventional cellulose assay according to Updegraff. Slightly lower cellulose levels were observed for Avicel (97%), corn stover (97%), poplar (96%) and Miscanthus (94%) but for pine the amounts were almost identical (101%). Despite these differences, the one‐step/two‐step method can be seen as true alternative to the more labor‐intensive Updegraff method. Biotechnol. Bioeng. © 2014 Wiley Periodicals, Inc. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - June 7, 2014 Category: Biomedical Science Authors: Stefan Bauer, Ana B. Ibáñez Tags: Communication to the Editor Source Type: research

Comparison of three local frame definitions for the kinematic analysis of the fingers and the wrist
(Source: Journal of Biomechanics)
Source: Journal of Biomechanics - June 7, 2014 Category: Biomedical Science Authors: B. Goislard de Monsabert, J.M.A. Visser, L. Vigouroux, F.C.T. Van der Helm, H.E.J. Veeger Source Type: research

Segment Lengths Influence Hill Walking Strategies
(Source: Journal of Biomechanics)
Source: Journal of Biomechanics - June 7, 2014 Category: Biomedical Science Authors: Riley C. Sheehan, Jinger S. Gottschall Source Type: research

Orthoses posted in both the forefoot and rearfoot reduce moments and angular impulses on lower extremity joints during walking
(Source: Journal of Biomechanics)
Source: Journal of Biomechanics - June 7, 2014 Category: Biomedical Science Authors: Wen-Hao Hsu, Cara L. Lewis, Gail M. Monaghan, Elliot Saltzman, Joseph Hamill, Kenneth G. Holt Source Type: research

Modeling the biomechanics of swine mastication – an inverse Dynamics approach
(Source: Journal of Biomechanics)
Source: Journal of Biomechanics - June 7, 2014 Category: Biomedical Science Authors: Ehsan Basafa, Ryan J. Murphy, Chad R. Gordon, Mehran Armand Source Type: research

Pulmonary Arterial Hypertension and Sepsis: Prothrombotic profile and inflammation can changes pulmonary mechanics?
(Source: Medical Hypotheses)
Source: Medical Hypotheses - June 7, 2014 Category: Biomedical Science Authors: Karla Mette Waldrich Tauil, Eduardo Ventura Gaio dos Santos, César Augusto Melo-Silva, Rodrigo Storck de Carvalho, Veronica Moreira Amado Source Type: research

Elimination Communication as Colic Therapy
(Source: Medical Hypotheses)
Source: Medical Hypotheses - June 7, 2014 Category: Biomedical Science Authors: Geraldine J. Jordan Source Type: research

Childhood attachment and schizophrenia: the “attachment–developmental-cognitive” (ADC) hypothesis
(Source: Medical Hypotheses)
Source: Medical Hypotheses - June 7, 2014 Category: Biomedical Science Authors: Ravi Philip Rajkumar Source Type: research

Remote ischemic preconditioning as prevention of transfusion-related acute lung injury
(Source: Medical Hypotheses)
Source: Medical Hypotheses - June 7, 2014 Category: Biomedical Science Authors: Carlos R. Camara-Lemarroy Source Type: research

Could have hosted and trained to evade innate immune response?
(Source: Medical Hypotheses)
Source: Medical Hypotheses - June 7, 2014 Category: Biomedical Science Authors: Khalid Ahmed Source Type: research

Childhood attachment and schizophrenia: The “attachment-developmental-cognitive” (ADC) hypothesis
(Source: Medical Hypotheses)
Source: Medical Hypotheses - June 7, 2014 Category: Biomedical Science Authors: Ravi Philip Rajkumar Source Type: research

[Correspondence] Venezuela: violence, human rights, and health-care realities
The Venezuelan National Academy of Medicine (VNAM) was founded in 1904 to act as a consulting body in all matters related to national medical science. This organisation is public, apolitical, scientific, and constitutes the most authoritative institution in Venezuela to provide recommendations in medical science. As such, we feel obliged to reply to Ewan Robertson's inaccurate and somewhat biased report on Venezuela to provide a more balanced view of the real suffering of the Venezuelan people. (Source: LANCET)
Source: LANCET - June 6, 2014 Category: Journals (General) Authors: Rafael Muci-Mendoza Tags: Correspondence Source Type: research

Herpes B virus gD interaction with its human receptor - an in silico analysis approach
Conclusion: In this study, we present structural model for the BV gD in a complex with its receptor. Some features predicted by this model can explain previously reported experimental data. This complex model may lead to a better understanding of the function of BV gD and its interaction with receptor and will improve our understanding of the activation of the BV fusion and entry process. (Source: Theoretical Biology and Medical Modelling)
Source: Theoretical Biology and Medical Modelling - June 6, 2014 Category: Biomedical Science Authors: Lingke LiZhengliang QiuYan LiFeng LiangHuahu YeYongqin CaiWanfeng GuoYan LiJunjie Yue Source Type: research

Multiscale fluid-structure interaction modelling to determine the mechanical stimulation of bone cells in a tissue engineered scaffold.
In this study, we seek to determine the mechanical stimulation of osteoblasts seeded in a biomaterial scaffold under flow perfusion and mechanical compression using multiscale modelling by two-way fluid-structure interaction and FE approaches. The mechanical stimulation, in terms of wall shear stress (WSS) and strain in osteoblasts, is quantified at different locations within the scaffold for cells of different attachment morphologies (attached, bridged). The results show that 75.4 % of scaffold surface has a WSS of 0.1-10 mPa, which indicates the likelihood of bone cell differentiation at these locations. For attached a...
Source: Biomechanics and Modeling in Mechanobiology - June 6, 2014 Category: Biomedical Science Authors: Zhao F, Vaughan TJ, Mcnamara LM Tags: Biomech Model Mechanobiol Source Type: research

Simultaneous determination of three dipeptides (JBP485, Gly-Sar and JBP923) in the cell lysates by liquid chromatography-tandem mass spectrometry: application to identify the function of the PEPT1 transfected cell.
Abstract A simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of JBP485, Gly-Sar and JBP923 in the cell lysates using methanol as a deproteinization solvent was developed and validated. Detection was performed by turbo ionspray ionization in multiple reaction monitoring mode using the transitions of m/z 147.1 → m/z 90.1 for Gly-Sar, m/z 201.1 → m/z 86.1 for JBP485, m/z 219.1 → m/z 86.1 for JBP923 and m/z 152.0 → m/z 110.0 for paracetamol (internal standard). The analytes were separated on a Hypersil ODS C18 HPLC column using i...
Source: Biomedical Chromatography : BMC - June 6, 2014 Category: Biomedical Science Authors: Guo X, Meng Q, Liu Q, Wang C, Huo X, Zhang Z, Kaku T, Liu K Tags: Biomed Chromatogr Source Type: research

Herpes B virus gD interaction with its human receptor ¿ an in silico analysis approach
Conclusion: In this study, we present structural model for the BV gD in a complex with its receptor. Some features predicted by this model can explain previously reported experimental data. This complex model may lead to a better understanding of the function of BV gD and its interaction with receptor and will improve our understanding of the activation of the BV fusion and entry process. (Source: Theoretical Biology and Medical Modelling)
Source: Theoretical Biology and Medical Modelling - June 6, 2014 Category: Biomedical Science Authors: Lingke LiZhengliang QiuYan LiFeng LiangHuahu YeYongqin CaiWanfeng GuoYan LiJunjie Yue Source Type: research

Guidance for Industry and FDA Staff: IND Applications for Minimally Manipulated, Unrelated Allogeneic Placental/Umbilical Cord Blood Intended for Hematopoietic and Immunologic Reconstitution in Patients with Disorders Affecting the Hematopoietic System
(Source: What's New at CBER)
Source: What's New at CBER - June 5, 2014 Category: Biomedical Science Source Type: news

Guidance for Industry: BLA for Minimally Manipulated, Unrelated Allogeneic Placental/Umbilical Cord Blood Intended for Hematopoietic and Immunologic Reconstitution in Patients with Disorders Affecting the Hematopoietic System
(Source: What's New at CBER)
Source: What's New at CBER - June 5, 2014 Category: Biomedical Science Source Type: news

AUDIO: Baby poisoning bug 'hard to treat'
Biomedical scientist Dr Ron Cutler says a bug which killed one baby and poisoned 14 others is 'difficult to treat'. (Source: BBC News | Health | UK Edition)
Source: BBC News | Health | UK Edition - June 5, 2014 Category: Consumer Health News Source Type: news

Quantification of larval zebrafish motor function in multiwell plates using open-source MATLAB applications
Nature Protocols 9, 1533 (2014). doi:10.1038/nprot.2014.094 Authors: Yangzhong Zhou, Richard T Cattley, Clinton L Cario, Qing Bai & Edward A Burton (Source: Nature Protocols)
Source: Nature Protocols - June 5, 2014 Category: Biomedical Science Authors: Yangzhong ZhouRichard T CattleyClinton L CarioQing BaiEdward A Burton Tags: Protocol Source Type: research

Tobacco rattle virus–based virus-induced gene silencing in Nicotiana benthamiana
Nature Protocols 9, 1549 (2014). doi:10.1038/nprot.2014.092 Authors: Muthappa Senthil-Kumar & Kirankumar S Mysore (Source: Nature Protocols)
Source: Nature Protocols - June 5, 2014 Category: Biomedical Science Authors: Muthappa Senthil-KumarKirankumar S Mysore Tags: Protocol Source Type: research

In vivo antigen-driven plasmablast enrichment in combination with antigen-specific cell sorting to facilitate the isolation of rare monoclonal antibodies from human B cells
Nature Protocols 9, 1563 (2014). doi:10.1038/nprot.2014.104 Authors: Zhonghua Lin, Nancy Y Chiang, Ning Chai, Dhaya Seshasayee, Wyne P Lee, Mercedesz Balazs, Gerald Nakamura & Lee R Swem (Source: Nature Protocols)
Source: Nature Protocols - June 5, 2014 Category: Biomedical Science Authors: Zhonghua LinNancy Y ChiangNing ChaiDhaya SeshasayeeWyne P LeeMercedesz BalazsGerald NakamuraLee R Swem Tags: Protocol Source Type: research

Liposome display for in vitro selection and evolution of membrane proteins
Nature Protocols 9, 1578 (2014). doi:10.1038/nprot.2014.107 Authors: Satoshi Fujii, Tomoaki Matsuura, Takeshi Sunami, Takehiro Nishikawa, Yasuaki Kazuta & Tetsuya Yomo (Source: Nature Protocols)
Source: Nature Protocols - June 5, 2014 Category: Biomedical Science Authors: Satoshi FujiiTomoaki MatsuuraTakeshi SunamiTakehiro NishikawaYasuaki KazutaTetsuya Yomo Tags: Protocol Source Type: research

The Institute for Clinical and Translational Science at UC Irvine: Building an Inquisitive Environment Where Everything Is Questioned and There Is No Status Quo
(Source: Clinical and Translational Science)
Source: Clinical and Translational Science - June 5, 2014 Category: Biomedical Science Authors: Vince Caiozzo, Dan M. Cooper, Steve Cramer, Pietro Galassetti, Ruth Mulnard, Danh Nguyen, Dele Ogunseitan, Ellen Olshansky, Andria Pontello, Margaret Schneider Tags: CTSA Profile Source Type: research

Spatial optimization in perfusion bioreactors improves bone tissue‐engineered construct quality attributes
This study demonstrates that the bioreactor chamber environment, incorporating a scaffold and its location within it, affects the flow patterns within the pores throughout the scaffold requiring therefore dedicated optimization that can lead to bone tissue engineered constructs with improved quality attributes. Biotechnol. Bioeng. © 2014 Wiley Periodicals, Inc. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - June 5, 2014 Category: Biomedical Science Authors: Ioannis Papantoniou, Yann Guyot, Maarten Sonnaert, Greet Kerckhofs, Frank P. Luyten, Liesbet Geris, Jan Schrooten Tags: Article Source Type: research

Carbon storage in recombinant Escherichia coli during growth on glycerol and lactic acid
Abstract A fed‐batch process was studied with lactate and glycerol supply in the growth phase and glycerol supply during L‐phenylalanine production with recombinant E. coli K‐12. Lactic acid feeding was necessary for growth because the genes encoding the PEP‐consuming pyruvate kinase isoenzymes (pykA, pykF) have been deleted. An unexpected glucose efflux (67.6 ± 2.3 mgGlucose gCDW−1) was measured after the cells were harvested and resuspended in a mineral medium for metabolic perturbation experiments. As the efflux prohibited the application of these experiments, characterization of intracellular carbon st...
Source: Biotechnology and Bioengineering - June 5, 2014 Category: Biomedical Science Authors: Michael Weiner, Julia Tröndle, Christoph Albermann, Georg A. Sprenger, Dirk Weuster‐Botz Tags: Article Source Type: research

Identification of critical parameters in liquid enzyme‐catalyzed biodiesel production
This study presents an investigation of the impact of temperature, enzyme and water concentration on the reaction, as well as the effect of methanol feed rate for the conversion of rapeseed oil in a fed‐batch reaction system. It was observed that the reaction can be divided into two distinct parts. The first part of the reaction, during which primarily tri‐ and diglycerides are converted, proceeded at a high rate and thus required a high rate of methanol supply. The second part of the reaction, where the remaining di‐ and monoglycerides are converted, proceeded at a much lower rate. Consequently, it is necessary to r...
Source: Biotechnology and Bioengineering - June 5, 2014 Category: Biomedical Science Authors: Mathias Nordblad, Vanessa T. L. Silva, Per Munk Nielsen, John M. Woodley Tags: Article Source Type: research

Lessons from diversity of directed evolution experiments by an analysis of 3000 mutations
Abstract Diversity generation by random mutagenesis is often the first key step in directed evolution experiments and screening of 1000‐2000 clones is in most directed evolution campaigns sufficient to identify improved variants. For experimentalists important questions such as how many positions are mutated in the targeted gene and what amino acid substitutions can be expected after screening of 1000‐2000 clones are surprisingly not answered by a statistical analysis of mutant libraries. Therefore three random mutagenesis experiments (epPCR with a low‐ and a high‐mutation frequency and a transversion‐enriched se...
Source: Biotechnology and Bioengineering - June 5, 2014 Category: Biomedical Science Authors: Jing Zhao, Tsvetan Kardashliev, Anna Joëlle Ruff, Marco Bocola, Ulrich Schwaneberg Tags: Article Source Type: research

Cell retention by encapsulation for the cultivation of Jurkat cells in fixed and fluidized bed reactors
Abstract Jurkat cells are accepted model cells for primary human T lymphocytes, e.g. in medical research. Their growth to tissue‐like cell densities (up to 100 × 106 cells/mLcapsule) in semi‐permeable (molecular weight cut off < 10'000 Da) sodium cellulose sulfate / poly(diallyldimethylammonium chloride) polyelectrolyte capsules has previously been shown by us (Werner et al. 2013). Herein, we demonstrate that encapsulation can be used to retain the cells in continuously operated bioreactors, which opens new possibilities for research, e.g. the use of Jurkat cells in pulse response experiments under steady s...
Source: Biotechnology and Bioengineering - June 5, 2014 Category: Biomedical Science Authors: P. Kaiser, M. Werner, V. Jérôme, H. Hübner, R. Buchholz, R. Freitag Tags: Article Source Type: research

Mechanics and spiral formation in the rat cornea.
Abstract During the maturation of some mammals such as mice and rats, corneal epithelial cells tend to develop into patterns such as spirals over time. A better understanding of these patterns can help to understand how the organ develops and may give insight into some of the diseases affecting corneal development. In this paper, a framework for explaining the development of the epithelial cells forming spiral patterns due to the effect of tensile and shear strains is proposed. Using chimeric animals, made by combining embryonic cells from genetically distinguishable strains, we can observe the development of patte...
Source: Biomechanics and Modeling in Mechanobiology - June 5, 2014 Category: Biomedical Science Authors: Mohammad Nejad T, Iannaccone S, Rutherford W, Iannaccone PM, Foster CD Tags: Biomech Model Mechanobiol Source Type: research

Identification of the metabolites of gigantol in rat urine by ultra-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry.
Abstract Gigantol is a typical bibenzyl compound isolated from Dendrobii Caulis that has been widely used as a medicinal herb in China for the treatment of diabetic cataract, cancer and arteriosclerosis obliterans and as a tonic for stomach nourishment, saliva secretion promotion and fever reduction. However, few studies have been carried out on its in vivo metabolism. In the present study, a rapid and sensitive method based on ultra-performance liquid chromatography/electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UPLC-Q/TOF-MS) in positive ion mode was developed and applied to identify ...
Source: Biomedical Chromatography : BMC - June 5, 2014 Category: Biomedical Science Authors: Fan Y, Han H, He C, Yang L, Wang Z Tags: Biomed Chromatogr Source Type: research

HILIC-MS-based shotgun metabolomic profiling of maternal urine at 9-23 weeks of gestation - establishing the baseline changes in the maternal metabolome.
Abstract In this data-rich age it is no longer necessary to methodically isolate, characterize and measure specific molecules. What is important is to identify which of the hundreds or thousands of resolved and measured 'unknown' molecules are potentially associated with the pathophysiology of interest. We have taken LC-MS data from pregnancy urine and applied SIMCA P+ data analysis software in shotgun metabolomics to search the large amount of data for significant metabolite changes that occur in the transition from the first to early second trimester of pregnancy. Seventy-two individual urine samples were examine...
Source: Biomedical Chromatography : BMC - June 5, 2014 Category: Biomedical Science Authors: Trivedi DK, Iles RK Tags: Biomed Chromatogr Source Type: research

Application of a sensitive and specific LC-MS/MS method for determination of eriodictyol-8-C-β-d-glucopyranoside in rat plasma for a bioavailability study.
This study is the first to detail the development and validation of a rapid, sensitive and specific LC-ESI-MS/MS method for the determination of eriodictyol-8-C-β-d-glucopyranoside (EG) in rat plasma. A simple protein precipitation method was used for plasma sample preparation. Chromatographic separation was successfully achieved on an Agilent Zorbax XDB C18 column (2.1 × 50 mm, 3.5 µm) using a step gradient program with the mobile phase of 0.1% formic acid aqueous solution and acetonitrile with 0.1% formic acid. EG and the internal standard (IS) were detected using an electrospray negative ionization mass spect...
Source: Biomedical Chromatography : BMC - June 5, 2014 Category: Biomedical Science Authors: Qiu F, Fu S, Zhang X, Gong M, Wang M Tags: Biomed Chromatogr Source Type: research

Simultaneous determination and pharmacokinetic study of three isoflavones from Trifolium pratense extract in rat plasma by LC-MS/MS.
Abstract A highly selective and sensitive liquid chromatography-tandem mass spectrometry has been developed and validated for simultaneous determination of three isoflavones - ononin, formononetin and biochanin A - in rat plasma using lysionotin as internal standard (IS). The plasma samples were pretreated and extracted by liquid-liquid extraction. Chromatographic separation was accomplished on a C18 column with the column temperature of 30 °C and a mobile phase of methanol-0.1% formic acid (75:25, v/v). The detection was accomplished by multiple-reaction monitoring scanning with positive/negative ion-switching ...
Source: Biomedical Chromatography : BMC - June 5, 2014 Category: Biomedical Science Authors: Zhang R, Wang S, Lu M, Zhang Z, Tian X, Lv D Tags: Biomed Chromatogr Source Type: research

Facile LC-UV methods for simultaneous monitoring of ciprofloxacin and rosuvastatin in API, formulations and human serum.
Abstract An efficient, selective and cost-effective liquid chromatographic assay was developed and validated for the simultaneous quantification of ciprofloxacin and rosuvastatin in Active Pharmaceutical Ingredients (API), pharmaceutical formulations and in human serum. The chromatographic system consisted of mobile phase methanol-water, 90:10 v/v at pH 3.0 adjusted with o-phosphoric acid, pumped at 1.0 mL/min through a prepacked Purospher Star C18 (5 µm, 25 × 0.46 cm) column and effluent was monitored at the isosbestic point (255 nm) as well as at the λmax of individual drugs (243 and 271 nm)....
Source: Biomedical Chromatography : BMC - June 5, 2014 Category: Biomedical Science Authors: Arayne MS, Sultana N, Tabassum A Tags: Biomed Chromatogr Source Type: research

Simultaneous determination of cinnamaldehyde and its metabolite in rat tissues by gas chromatography-mass spectrometry.
Abstract Cinnamaldehyde (CA), an active ingredient isolated from the traditional Chinese medicine Cortex Cinnamomi, has a wide range of bioactivities. To clarify the distribution characteristics of CA, a selective and sensitive method utilizing gas chromatography-mass spetrometry was initially developed for simultaneously determining the concentration of CA and its metabolite cinnamyl alcohol in rat tissues. Selected ion masses of m/z 131, 105 and 92 were chosen, and separation of the analytes was performed on a DB-5 ms (30 m × 0.25 mm, 0.25 µm, thickness) capillary column by gas chromatography-mass s...
Source: Biomedical Chromatography : BMC - June 5, 2014 Category: Biomedical Science Authors: Zhao H, Yang Q, Xie Y, Sun J, Tu H, Cao W, Wang S Tags: Biomed Chromatogr Source Type: research

Simultaneous analysis of seven 2-hydroxy fatty acids as tert-butyldimethylsilyl derivatives in plasma by gas chromatography-mass spectrometry.
Abstract An efficient method for the simultaneous analysis of seven 2-hydroxy fatty acids (2-HFAs) as tert-butyldimethylsilyl (TBDMS) derivative was developed by gas chromatography-mass spectrometry in selected ion monitoring mode. New mass spectral data on 2-hydroxycapric, 2-hydroxypalmitic, 2-hydroxystearic and 2-hydroxybehenic acids as di-TBDMS derivatives for hydroxyl and carboxyl groups were built. Under the optimal conditions, the present method showed a good correlation coefficient (r ≥ 0.999) in the range of 0.01-0.5 µg. The precision showed low relative standard deviation of <10%, and the accur...
Source: Biomedical Chromatography : BMC - June 5, 2014 Category: Biomedical Science Authors: Seo C, Yoon J, Rhee Y, Kim JJ, Nam SJ, Lee W, Lee G, Yee ST, Paik MJ Tags: Biomed Chromatogr Source Type: research

Analysis and pharmacokinetic study of polyphyllin H in beagle dog plasma after oral administration of Rhizoma Paridis extracts by LC-MS/MS.
Abstract A highly sensitive, rapid assay method has been developed and validated for the analysis of polyphyllin H in beagle dog plasma with liquid chromatography coupled to tandem mass spectrometry with electrospray ionization in the positive-ion mode. The assay procedure involves extraction of polyphyllin H and ginsenoside Re (IS) from beagle dog plasma. Chromatographic separation was carried out on an Agilent Zorbax XDB-C18 (100 × 2.1 mm, 1.8μm) column by isocratic elution with acetonitrile and water (50:50, v/v) at a flow rate of 0.25 mL/min with a total run time of 2.5 min. The MS/MS ion transitions moni...
Source: Biomedical Chromatography : BMC - June 5, 2014 Category: Biomedical Science Authors: Yin X, Lin L, Shen M, Zhai Y, Cao S, Fu J, Li X, Yang C, Xia Z, Zhao Y, Li S, Bai Y, Xue D, Ni J Tags: Biomed Chromatogr Source Type: research

Strategy for automated NMR resonance assignment of RNA: application to 48-nucleotide K10.
Abstract A procedure is presented for automated sequence-specific assignment of NMR resonances of uniformly [(13)C, (15)N]-labeled RNA. The method is based on a suite of four through-bond and two through-space high-dimensional automated projection spectroscopy (APSY) experiments. The approach is exemplified with a 0.3 mM sample of an RNA stem-loop with 48 nucleotides, K10, which is responsible for dynein-mediated localization of Drosophila fs(1)K10 mRNA transcripts. The automated analysis of the APSY data led to highly accurate and precise 3- to 4-dimensional peak lists. They provided a reliable basis for the subs...
Source: Journal of Bimolecular NMR - June 5, 2014 Category: Biomedical Science Authors: Krähenbühl B, Lukavsky P, Wider G Tags: J Biomol NMR Source Type: research

Is torso soft tissue motion really an artefact within breast biomechanics research?
(Source: Journal of Biomechanics)
Source: Journal of Biomechanics - June 5, 2014 Category: Biomedical Science Authors: Chris Mills, Amy Loveridge, Alexandra Milligan, Debbie Risius, Joanna Scurr Source Type: research

Dissecting the contribution of actin and vimentin intermediate filaments to mechanical phenotype of suspended cells using High-throughput deformability measurements and computational modeling
(Source: Journal of Biomechanics)
Source: Journal of Biomechanics - June 5, 2014 Category: Biomedical Science Authors: Evgeny Gladilin, Paula Gonzalez, Roland Eils Source Type: research

Nucleotomy Reduces the Effects of Cyclic Compressive Loading with Unloaded Recovery on Human Intervertebral Discs
(Source: Journal of Biomechanics)
Source: Journal of Biomechanics - June 5, 2014 Category: Biomedical Science Authors: Brent L. Showalter, Neil R. Malhotra, Edward J. Vresilovic, Dawn M. Elliott Source Type: research

Effects of footwear on three-dimensional tibiotalar and subtalar joint motion during running
(Source: Journal of Biomechanics)
Source: Journal of Biomechanics - June 5, 2014 Category: Biomedical Science Authors: Cathryn D. Peltz, Jeffrey A. Haladik, Scott E. Hoffman, Michael McDonald, Nicole L. Ramo, George Divine, Matthew Nurse, Michael J. Bey Source Type: research