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Biomedical Science

This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 9.

Editorial Board and Publication Information
(Source: Journal of Biomechanics)
Source: Journal of Biomechanics - June 3, 2014 Category: Biomedical Science Source Type: research

The use of high pressure CO2‐facilitated pH swings to enhance in situ product recovery of butyric acid in a two‐phase partitioning bioreactor
ABSTRACT Through the use of high partial pressures of CO2 (pCO2) to achieve temporary pH reductions in two‐phase partitioning bioreactors (TPPBs), improved pH dependent partitioning of butyric acid was observed which achieved in situ product recovery (ISPR), alleviating end‐product inhibition (EPI) during the production of butyric acid by Clostridium tyrobutyricum (ATCC 25755). Through high pressure pCO2 studies, media buffering effects were shown to be substantially overcome at 60 bar pCO2, resulting in effective extraction of the organic acid by the absorptive polymer Pebax® 2533, yielding a distribution coefficient...
Source: Biotechnology and Bioengineering - June 3, 2014 Category: Biomedical Science Authors: Eric C. Peterson, Andrew J. Daugulis Tags: Article Source Type: research

NOTCH inhibits osteoblast formation in inflammatory arthritis via noncanonical NF-κB
NOTCH-dependent signaling pathways are critical for normal bone remodeling; however, it is unclear if dysfunctional NOTCH activation contributes to inflammation-mediated bone loss, as observed in rheumatoid arthritis (RA) patients. We performed RNA sequencing and pathway analyses in mesenchymal stem cells (MSCs) isolated from transgenic TNF-expressing mice, a model of RA, to identify pathways responsible for decreased osteoblast differentiation. 53 pathways were dysregulated in MSCs from RA mice, among which expression of genes encoding NOTCH pathway members and members of the noncanonical NF-κB pathway were markedly elev...
Source: Journal of Clinical Investigation - June 3, 2014 Category: Biomedical Science Authors: Hengwei Zhang, Matthew J. Hilton, Jennifer H. Anolik, Stephen L. Welle, Chen Zhao, Zhenqiang Yao, Xing Li, Zhiyu Wang, Brendan F. Boyce, Lianping Xing Source Type: research

Myosin Vb uncoupling from RAB8A and RAB11A elicits microvillus inclusion disease
Microvillus inclusion disease (MVID) is a severe form of congenital diarrhea that arises from inactivating mutations in the gene encoding myosin Vb (MYO5B). We have examined the association of mutations in MYO5B and disruption of microvillar assembly and polarity in enterocytes. Stable MYO5B knockdown (MYO5B-KD) in CaCo2-BBE cells elicited loss of microvilli, alterations in junctional claudins, and disruption of apical and basolateral trafficking; however, no microvillus inclusions were observed in MYO5B-KD cells. Expression of WT MYO5B in MYO5B-KD cells restored microvilli; however, expression of MYO5B-P660L, a MVID-assoc...
Source: Journal of Clinical Investigation - June 3, 2014 Category: Biomedical Science Authors: Byron C. Knowles, Joseph T. Roland, Moorthy Krishnan, Matthew J. Tyska, Lynne A. Lapierre, Paul S. Dickman, James R. Goldenring, Mitchell D. Shub Source Type: research

CTLA4 aptamer delivers STAT3 siRNA to tumor-associated and malignant T cells
Intracellular therapeutic targets that define tumor immunosuppression in both tumor cells and T cells remain intractable. Here, we have shown that administration of a covalently linked siRNA to an aptamer (apt) that selectively binds cytotoxic T lymphocyte–associated antigen 4 (CTLA4apt) allows gene silencing in exhausted CD8+ T cells and Tregs in tumors as well as CTLA4-expressing malignant T cells. CTLA4 expression was upregulated in CD8+ T cells in the tumor milieu; therefore, CTLA4apt fused to a STAT3-targeting siRNA (CTLA4apt–STAT3 siRNA) resulted in internalization into tumor-associated CD8+ T cells and silencing...
Source: Journal of Clinical Investigation - June 3, 2014 Category: Biomedical Science Authors: Andreas Herrmann, Saul J. Priceman, Maciej Kujawski, Hong Xin, Gregory A. Cherryholmes, Wang Zhang, Chunyan Zhang, Christoph Lahtz, Claudia Kowolik, Steve J. Forman, Marcin Kortylewski, Hua Yu Source Type: research

Cystic fibrosis airway secretions exhibit mucin hyperconcentration and increased osmotic pressure
The pathogenesis of mucoinfective lung disease in cystic fibrosis (CF) patients likely involves poor mucus clearance. A recent model of mucus clearance predicts that mucus flow depends on the relative mucin concentration of the mucus layer compared with that of the periciliary layer; however, mucin concentrations have been difficult to measure in CF secretions. Here, we have shown that the concentration of mucin in CF sputum is low when measured by immunologically based techniques, and mass spectrometric analyses of CF mucins revealed mucin cleavage at antibody recognition sites. Using physical size exclusion chromatograph...
Source: Journal of Clinical Investigation - June 3, 2014 Category: Biomedical Science Authors: Ashley G. Henderson, Camille Ehre, Brian Button, Lubna H. Abdullah, Li-Heng Cai, Margaret W. Leigh, Genevieve C. DeMaria, Hiro Matsui, Scott H. Donaldson, C. William Davis, John K. Sheehan, Richard C. Boucher, Mehmet Kesimer Source Type: research

Biliary repair and carcinogenesis are mediated by IL-33–dependent cholangiocyte proliferation
Injury to the biliary epithelium triggers inflammation and fibrosis, which can result in severe liver diseases and may progress to malignancy. Development of a type 1 immune response has been linked to biliary injury pathogenesis; however, a subset of patients with biliary atresia, the most common childhood cholangiopathy, exhibit increased levels of Th2-promoting cytokines. The relationship among different inflammatory drivers, epithelial repair, and carcinogenesis remains unclear. Here, we determined that the Th2-activating cytokine IL-33 is elevated in biliary atresia patient serum and in the livers and bile ducts of mi...
Source: Journal of Clinical Investigation - June 3, 2014 Category: Biomedical Science Authors: Jun Li, Nataliya Razumilava, Gregory J. Gores, Stephanie Walters, Tatsuki Mizuochi, Reena Mourya, Kazuhiko Bessho, Yui-Hsi Wang, Shannon S. Glaser, Pranavkumar Shivakumar, Jorge A. Bezerra Source Type: research

Abnormal B cell memory subsets dominate HIV-specific responses in infected individuals
Recently, several neutralizing anti-HIV antibodies have been isolated from memory B cells of HIV-infected individuals. Despite extensive evidence of B cell dysfunction in HIV disease, little is known about the cells from which these rare HIV-specific antibodies originate. Accordingly, we used HIV envelope gp140 and CD4 or coreceptor (CoR) binding site (bs) mutant probes to evaluate HIV-specific responses in peripheral blood B cells of HIV-infected individuals at various stages of infection. In contrast to non-HIV responses, HIV-specific responses against gp140 were enriched within abnormal B cells, namely activated and exh...
Source: Journal of Clinical Investigation - June 3, 2014 Category: Biomedical Science Authors: Lela Kardava, Susan Moir, Naisha Shah, Wei Wang, Richard Wilson, Clarisa M. Buckner, Brian H. Santich, Leo J.Y. Kim, Emily E. Spurlin, Amy K. Nelson, Adam K. Wheatley, Christopher J. Harvey, Adrian B. McDermott, Kai W. Wucherpfennig, Tae-Wook Chun, John S Source Type: research

Physical Ischemia Induced by Isometric Exercise Facilitated Collateral Development in the Remote Ischemic Myocardium of Humans
Conclusions: Isometric handgrip exercise-induced physical ischemia training may promote remote collateral recruitment and growth in CAD patients. (Source: Clinical Science)
Source: Clinical Science - June 3, 2014 Category: Biomedical Science Authors: S Lin, Y Chen, Y Li, J Li, X Lu Source Type: research

A simple method for eliminating fixed‐region interference of aptamer binding during SELEX
Abstract Standard libraries for systematic evolution of ligands by exponential enrichment (SELEX) typically utilize flanking regions that facilitate amplification of aptamers recovered from each selection round. Here we show that these flanking sequences can bias the selection process, due in part to their ability to interfere with the fold or function of aptamers localized within the random region of the library sequence. We then address this problem by investigating the use of complementary oligonucleotides as a means to block aptamer interference by each flanking region. Isothermal titration calorimetry (ITC) studies ar...
Source: Biotechnology and Bioengineering - June 3, 2014 Category: Biomedical Science Authors: Eric Ouellet, Eric T. Lagally, Karen C. Cheung, Charles A. Haynes Tags: Article Source Type: research

A biomimetic honeycomb‐like scaffold prepared by flow‐focusing technology for cartilage regeneration
In conclusion, a highly ordered and honeycomb‐like scaffold shows superior performance in cartilage tissue engineering. Biotechnol. Bioeng. © 2014 Wiley Periodicals, Inc. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - June 3, 2014 Category: Biomedical Science Authors: Chen‐Chie Wang, Kai‐Chiang Yang, Keng‐Hui Lin, Chang‐Chin Wu, Yen‐Liang Liu, Feng‐Huei Lin, Ing‐Ho Chen Tags: Article Source Type: research

Biofilm growth of Chlorella sorokiniana in a rotating biological contactor based photobioreactor
In this study, a photobioreactor design based on a rotating biological contactor (RBC) was used as a production platform for microalgae biomass cultivated in biofilm. In the photobioreactor, referred to as Algadisk, microalgae grow in biofilm on vertical rotating disks partially submerged in a growth medium. The objective is to evaluate the potential of the Algadisk photobioreactor with respect to the effects of disk roughness, disk rotation speed and CO2 concentration. These objectives where evaluated in relationship to productivity, photosynthetic efficiency, and long‐term cultivation stability in a lab‐scale Algadis...
Source: Biotechnology and Bioengineering - June 3, 2014 Category: Biomedical Science Authors: W. Blanken, M. Janssen, M. Cuaresma, Z. Libor, T. Bhaiji, R.H. Wijffels Tags: Article Source Type: research

Enhancing fatty acid ethyl ester production in Saccharomyces cerevisiae through metabolic engineering and medium optimization
ABSTRACT Biodiesels in the form of fatty acyl ethyl esters (FAEEs) are a promising next generation biofuel due to their chemical properties and compatibility with existing infrastructure. It has recently been shown that expression of a bacterial acyl‐transferase in the established industrial workhorse Saccharomyces cerevisiae can lead to production of FAEEs by condensation of fatty acyl‐CoAs and ethanol. In contrast to recent strategies to produce FAEEs in S. cerevisiae through manipulation of de novo fatty acid biosynthesis or a series of arduous genetic manipulations, we introduced a novel genetic background which is...
Source: Biotechnology and Bioengineering - June 3, 2014 Category: Biomedical Science Authors: R. Adam Thompson, Cong T. Trinh Tags: Article Source Type: research

Direct biosynthesis of adipic acid from a synthetic pathway in recombinant Escherichia coli
Abstract The C6 dicarboxylic acid, adipic acid, is an important platform chemical in industry. Biobased production of adipic acid is a promising alternative to the current petrochemical route. Here we report biosynthesis of adipic acid using an artificial pathway inspired by the reversal of beta‐oxidation of dicarboxylic acids. The biosynthetic pathway comprises condensation of acetyl‐CoA and succinyl‐CoA to form the C6 backbone and subsequent reduction, dehydration, hydrogenation, and release of adipic acid from its thioester. The pathway was first tested in vitro with reconstituted pathway enzymes and then function...
Source: Biotechnology and Bioengineering - June 3, 2014 Category: Biomedical Science Authors: Jia‐Le Yu, Xiao‐Xia Xia, Jian‐Jiang Zhong, Zhi‐Gang Qian Tags: Communication to the Editor Source Type: research

A computational model that predicts reverse growth in response to mechanical unloading.
Abstract Ventricular growth is widely considered to be an important feature in the adverse progression of heart diseases, whereas reverse ventricular growth (or reverse remodeling) is often considered to be a favorable response to clinical intervention. In recent years, a number of theoretical models have been proposed to model the process of ventricular growth while little has been done to model its reverse. Based on the framework of volumetric strain-driven finite growth with a homeostatic equilibrium range for the elastic myofiber stretch, we propose here a reversible growth model capable of describing both vent...
Source: Biomechanics and Modeling in Mechanobiology - June 3, 2014 Category: Biomedical Science Authors: Lee LC, Genet M, Acevedo-Bolton G, Ordovas K, Guccione JM, Kuhl E Tags: Biomech Model Mechanobiol Source Type: research

Ca2+-stabilized adhesin helps an Antarctic bacterium reach out and bind ice.
Abstract The large size of a 1.5-MDa ice-binding adhesin (MpAFP) from an Antarctic Gram-negative bacterium, Marinomonas primoryensis, is mainly due to its highly repetitive Region II (RII). MpAFP_RII contains roughly 120 tandem copies of an identical 104-residue repeat. We have previously determined that a single RII repeat folds as a Ca2+-dependent immunoglobulin-like domain. Here, we solved the crystal structure of four tandem RII repeats (RII tetra-tandemer) to a resolution of 1.8 Å. The RII tetra-tandemer reveals an extended (~190-Å x ~25-Å), rod-like structure with four RII-repeats aligned in series with ea...
Source: Bioscience Reports - June 3, 2014 Category: Biomedical Science Authors: Vance TD, Olijve LL, Campbell RL, Voets IK, Davies PL, Guo S Tags: Biosci Rep Source Type: research

FKBP5 expression in human adipose tissue increases following dexamethasone exposure and is associated with insulin resistance
(Source: Metabolism - Clinical and Experimental)
Source: Metabolism - Clinical and Experimental - June 3, 2014 Category: Biomedical Science Authors: Maria J. Pereira, Jenny Palming, Maria K. Svensson, Magnus Rizell, Jan Dalenbäck, Mårten Hammar, Tove Fall, Cherno O. Sidibeh, Per-Arne Svensson, Jan W. Eriksson Source Type: research

DNA Methylation Is Altered In B And Nk Lymphocytes In Obese And Type 2 Diabetic Human
(Source: Metabolism - Clinical and Experimental)
Source: Metabolism - Clinical and Experimental - June 3, 2014 Category: Biomedical Science Authors: David Simar, Soetkin Versteyhe, Ida Donkin, Jia Liu, Luke Hesson, Vibe Nylander, Anna Fossum, Romain Barrès Source Type: research

Berberine inhibits cholesterol absorption in rats through multi-mechanisms
(Source: Metabolism - Clinical and Experimental)
Source: Metabolism - Clinical and Experimental - June 3, 2014 Category: Biomedical Science Authors: Yanwen Wang, Xin Yi, Khadija Ghanam, Shuocheng Zhang, Tiantian Zhao, Xuemei Zhu Source Type: research

Serum levels of soluble Receptor for Advanced Glycation End-products and metabolic syndrome: the Northern Manhattan Study
(Source: Metabolism - Clinical and Experimental)
Source: Metabolism - Clinical and Experimental - June 3, 2014 Category: Biomedical Science Authors: Barry I. Hudson, Chuanhui Dong, Hannah Gardener, Mitchell S.V. Elkind, Clinton B. Wright, Ron Goldberg, Ralph L. Sacco, Tatjana Rundek Source Type: research

Berberine decreases cholesterol levels in rats through multiple mechanisms, including inhibition of cholesterol absorption
(Source: Metabolism - Clinical and Experimental)
Source: Metabolism - Clinical and Experimental - June 3, 2014 Category: Biomedical Science Authors: Yanwen Wang, Xin Yi, Khadija Ghanam, Shuocheng Zhang, Tiantian Zhao, Xuemei Zhu Source Type: research

Postdoc Daniel Schramek awarded Regeneron Prize for “dream” biomedical project
Schramek, one of just two individuals receiving the annual award, is being recognized for his proposal of a project that used sequence-based personalized medicine to treat the most devastating features of cancer. The award comes with a $50,000 prize and a $5,000 donation to support seminars at Rockefeller. More » (Source: The Rockefeller University Newswire)
Source: The Rockefeller University Newswire - June 2, 2014 Category: Biomedical Science Authors: pubaff Tags: Awards and Honors cancer Daniel Schramek Elaine Fuchs MYH9 Regeneron Regeneron Prize for Creative Innovation Source Type: news

Daniel Schramek awarded Regeneron Prize for Creative Innovation by a Postdoctoral Fellow
Schramek, one of just two individuals receiving the annual award, is being recognized for his proposal of a project that used sequence-based personalized medicine to treat the most devastating features of cancer. The award comes with a $50,000 prize and a $5,000 donation to support seminars at Rockefeller. More » (Source: The Rockefeller University Newswire)
Source: The Rockefeller University Newswire - June 2, 2014 Category: Biomedical Science Authors: pubaff Tags: Awards and Honors cancer Daniel Schramek Elaine Fuchs MYH9 Regeneron Regeneron Prize for Creative Innovation Source Type: news

Editorial Board and Publication Information
(Source: Journal of Biomechanics)
Source: Journal of Biomechanics - June 2, 2014 Category: Biomedical Science Source Type: research

Introduction to the special issue on circadian rhythms in behavioral neuroscience.
Circadian rhythms influence virtually every aspect of behavior. This special issue provides updates on the current state and future directions for the field of circadian rhythms in behavioral neuroscience. Recent data have linked circadian rhythms to behavioral and physiological phenomena and disease states in virtually every area of behavioral and biomedical science. Specifically, a growing interest in the cognitive, neuropsychiatric, and metabolic consequences of circadian rhythm disorders and dysfunction is emerging. This special issue is not intended to provide an exhaustive overview of the entire field of chronobiolog...
Source: Behavioral Neuroscience - June 2, 2014 Category: Neuroscience Authors: Weil, Zachary M.; Nelson, Randy J. Source Type: research

CHOPPI: A web tool for the analysis of immunogenicity risk from host cell proteins in CHO‐based protein production
Abstract Despite high quality standards and continual process improvements in manufacturing, host cell protein (HCP) process impurities remains a substantial risk for biological products. Even at low levels, residual HCPs can induce a detrimental immune response compromising the safety and efficacy of a biologic. Consequently, advanced‐stage clinical trials have been cancelled due to the identification of antibodies against HCPs. To enable earlier and rapid assessment of the risks in Chinese Hamster Ovary (CHO)‐based protein production of residual CHO protein impurities (CHOPs), we have developed a web tool called CHOP...
Source: Biotechnology and Bioengineering - June 2, 2014 Category: Biomedical Science Authors: Chris Bailey‐Kellogg, Andres H. Gutiérrez, Leonard Moise, Frances Terry, William D. Martin, Anne S. De Groot Tags: Article Source Type: research

Defining an optimal Stromal Derived Factor‐1 presentation for effective recruitment of mesenchymal stem cells in 3D
Abstract In “situ” tissue engineering is a promising approach in regenerative medicine, envisaging to potentiate the physiological tissue repair processes by recruiting the host's own cellular progenitors at the lesion site by means of bioactive materials. Despite numerous works focused the attention in characterizing novel chemoattractant molecules, only few studied the optimal way to present signal in the microenvironment, in order to recruit cells more effectively. In this work, we have analyzed the effects of gradients of Stromal Derived Factor‐1 (SDF‐1) on the migratory behaviour of human Mesenchymal Stem Cell...
Source: Biotechnology and Bioengineering - June 2, 2014 Category: Biomedical Science Authors: Maria Iannone, Maurizio Ventre, Gemma Pagano, Paolo Giannoni, Rodolfo Quarto, Paolo Antonio Netti Tags: Article Source Type: research

The continuum heterogeneous biofilm model with multiple limiting substrate Monod kinetics
We describe a novel procedure to estimate the net growth rate of biofilms on multiple substrates. The approach is based on diffusion‐reaction mass balances for chemical species in a continuum biofilm model with reaction kinetics corresponding to a Double‐Monod expression. This analytical model considers a heterogeneous biofilm with variable distributions of biofilm density, activity and effective diffusivity as a function of depth. We present the procedure to estimate the effectiveness factor analytically and compare the outcome with values obtained by the application of a rigorous numerical computational method using ...
Source: Biotechnology and Bioengineering - June 2, 2014 Category: Biomedical Science Authors: Elio Emilio Gonzo, Stefan Wuertz, Veronica B. Rajal Tags: Article Source Type: research

Fractional factorial design to investigate stromal cell regulation of macrophage plasticity
In conclusion, fractional factorial experimental design identified stromal derived PGE2 as the factor most important in facilitating macrophage reprogramming, albeit via two unique pathways. Biotechnol. Bioeng. © 2014 Wiley Periodicals, Inc. (Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - June 2, 2014 Category: Biomedical Science Authors: Jeffrey A. Barminko, Nir I. Nativ, Rene Schloss, Martin L. Yarmush Tags: Article Source Type: research

Efficient odd straight medium chain free fatty acid production by metabolically engineered Escherichia coli
In this study, odd straight medium chain FFAs production was investigated by using metabolic engineered E. coli carrying acyl‐ACP thioesterase (TE, Ricinus communis), propionyl‐CoA synthase (Salmonella enterica) and β‐ketoacyl‐acyl carrier protein synthase III (four different sources) with supplement of extracellular propionate. By using these metabolically engineered E. coli, significant quantity of C13 and C15 odd straight‐chain FFAs could be produced from glucose and propionate. The highest concentration of total odd straight chain FFAs attained was 1205 mg/L by the strain HWK201 (pXZ18, pBHE2), and 85% of ...
Source: Biotechnology and Bioengineering - June 2, 2014 Category: Biomedical Science Authors: Hui Wu, Ka‐Yiu San Tags: Article Source Type: research

Systems metabolic engineering of Escherichia coli for gram scale production of the antitumor drug deoxyviolacein from glycerol
Abstract Deoxyviolacein is a microbial drug with biological activity against tumors, gram‐positive bacteria, and fungal plant pathogens. Here, we describe an Escherichia coli strain for heterologous production of this high‐value drug from glycerol. Plasmid‐based expression of the deoxyviolacein cluster vioABCE was controlled by the araBAD promoter and induction by L‐arabinose. Through elimination of L‐arabinose catabolism in E. coli, the pentose sugar could be fully directed to induction of deoxyviolacein biosynthesis and was no longer metabolized, as verified by 13C isotope experiments. Deletion of the araBAD ge...
Source: Biotechnology and Bioengineering - June 2, 2014 Category: Biomedical Science Authors: André Luis Rodrigues, Judith Becker, André Oliveira de Souza Lima, Luismar Marques Porto, Christoph Wittmann Tags: Article Source Type: research

High current densities enable exoelectrogens to outcompete aerobic heterotrophs for substrate
Abstract In mixed‐culture microbial fuel cells (MFCs), exoelectrogens and other microorganisms compete for substrate. It has previously been assumed that substrate losses to other terminal electron acceptors over a fed‐batch cycle, such as dissolved oxygen, are constant. However, a constant rate of substrate loss would only explain small increases in coulombic efficiencies (CEs, the fraction of substrate recovered as electrical current) with shorter cycle times, but not the large increases in CE that are usually observed with higher current densities and reduced cycle times. To better understand changes in CEs, COD con...
Source: Biotechnology and Bioengineering - June 2, 2014 Category: Biomedical Science Authors: Lijiao Ren, Xiaoyuan Zhang, Weihua He, Bruce E. Logan Tags: Article Source Type: research

Preparative crystallization of a single chain antibody using an aqueous two‐phase system
Abstract A simultaneous crystallization and aqueous two‐phase extraction of a single chain antibody was developed, demonstrating process integration. The process conditions were designed to form an aqueous two‐phase system, and to favor crystallization, using sodium sulfate and PEG‐2000. At sufficiently high concentrations of PEG, a second phase was generated in which the protein crystallization occurred simultaneously.The single chain antibody crystals were partitioned to the top, polyethylene glycol‐rich phase. The crystal nucleation took place in the sodium sulfate‐rich phase and at the phase boundary, whereas...
Source: Biotechnology and Bioengineering - June 2, 2014 Category: Biomedical Science Authors: Hauke Huettmann, Matthias Berkemeyer, Wolfgang Buchinger, Alois Jungbauer Tags: Article Source Type: research

Insulin receptor antibody‐iduronate 2‐sulfatase fusion protein: Pharmacokinetics, anti‐drug antibody, and safety pharmacology in Rhesus monkeys
ABSTRACT Mucopolysaccharidosis (MPS) Type II is caused by mutations in the gene encoding the lysosomal enzyme, iduronate 2‐sulfatase (IDS). The majority of MPSII cases affect the brain. However, enzyme replacement therapy with recombinant IDS does not treat the brain, because IDS is a large molecule drug that does not cross the blood‐brain barrier (BBB). To enable BBB penetration, IDS has been re‐engineered as an IgG‐IDS fusion protein, where the IgG domain is a monoclonal antibody (MAb) against the human insulin receptor (HIR). The HIRMAb crosses the BBB via receptor‐mediated transport on the endogenous BBB insu...
Source: Biotechnology and Bioengineering - June 2, 2014 Category: Biomedical Science Authors: Ruben J. Boado, Eric Ka‐Wai Hui, Jeff Zhiqiang Lu, William M. Pardridge Tags: Article Source Type: research

A microgroove patterned multiwell cell culture plate for high‐throughput studies of cell alignment
Abstract Grooved substrates are commonly used to guide cell alignment and produce in vitro tissues that mimic certain aspects of in vivo cellular organization. These more sophisticated tissues provide valuable in vitro models for testing drugs and for dissecting out molecular mechanisms that direct tissue organization. To increase the accessibility of these tissue models we describe a simple and yet reproducible strategy to produce 1 µm‐spaced grooved well plates suitable for conducting automated analysis of cellular responses. We characterize the alignment of four human cell types: retinal epithelial, umbilical vein ...
Source: Biotechnology and Bioengineering - June 2, 2014 Category: Biomedical Science Authors: Petra B. Lücker, Sahar Javaherian, John P. Soleas, Duncan Halverson, Peter W. Zandstra, Alison P. McGuigan Tags: Article Source Type: research

A simple, rapid and sensitive liquid chromatography-tandem mass spectrometry method for the determination of dienogest in human plasma and its pharmacokinetic applications under fasting.
Abstract A simple, rapid and sensitive analytical method using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) detection with positive ion electrospray ionization was developed for the determination of dienogest in human K2 EDTA plasma using levonorgestrel d6 as an internal standard (IS). Dienogest and IS were extracted from human plasma using simple liquid-liquid extraction. Chromatographic separation was achieved on a Zorbax XDB-Phenyl column (4.6 × 75 mm, 3.5 µm) under isocratic conditions using acetonitrile-5 mm ammonium acetate (70:30, v/v) at a flow rate of 0.60 mL/min. The ...
Source: Biomedical Chromatography : BMC - June 2, 2014 Category: Biomedical Science Authors: Pallapothu LM, Batta N, Pigili RK, Yejella RP Tags: Biomed Chromatogr Source Type: research

Simultaneous quantification of catecholamines in rat brain by high-performance liquid chromatography with on-line gold nanoparticle-catalyzed luminol chemiluminescence detection.
Abstract A new method based on high-performance liquid chromatography (HPLC) coupled with on-line gold nanoparticle-catalyzed luminol chemiluminescence (CL) detection was developed for the simultaneous quantitation of catecholamines in rat brain. In the present CL system, gold nanoparticles were produced by the on-line reaction of H2 O2 , NaHCO3 -Na2 CO3 (buffer solution of luminol) and HAuCl4. Norepinephrine (NE), epinephrine (EP) and dopamine (DA) could strongly enhance the CL signal of the on-line gold nanoparticle-catalyzed luminol system. The UV-visible absorption spectra and transmission electron microscopy s...
Source: Biomedical Chromatography : BMC - June 2, 2014 Category: Biomedical Science Authors: Mu C, Zhang Q, Wu D, Zhang Y, Zhang Q Tags: Biomed Chromatogr Source Type: research

Rapid determination of corticosterone in mouse plasma by ultra fast liquid chromatography-tandem mass spectrometry.
Abstract Major depressive disorder is a severe, life-threatening and highly prevalent psychiatric disorder. A high percentage of people suffering from depression are characterized by hyperactivity of the hypothalamic-pituitary-adrenal axis, resulting in plasma glucocorticoid (cortisol in human and corticosterone in rodent) elevations. Glucocorticoid is a critical molecule in the onset of pathology of depression. A simple, highly sensitive and specific method based on ultra-fast liquid chromatography-tandem mass spectrometry method has been developed for the quantitation of corticosterone in mouse plasma for the fir...
Source: Biomedical Chromatography : BMC - June 2, 2014 Category: Biomedical Science Authors: Li H, Liu X, Poh Y, Wu L, Zhou QG, Cai BC Tags: Biomed Chromatogr Source Type: research

Gas chromatography-electron ionization-mass spectrometry quantitation of valproic acid and gabapentin, using dried plasma spots, for therapeutic drug monitoring in in-home medical care.
Abstract A simple and sensitive gas chromatography-electron ionization-mass spectrometry (GC-EI-MS) method using dried plasma spot testing cards was developed for determination of valproic acid and gabapentin concentrations in human plasma from patients receiving in-home medical care. We have proposed that a simple, easy and dry sampling method is suitable for in-home medical patients for therapeutic drug monitoring. Therefore, in the present study, we used recently developed commercially available easy handling cards: Whatman FTA DMPK-A and Bond Elut DMS. In-home medical care patients can collect plasma using thes...
Source: Biomedical Chromatography : BMC - June 2, 2014 Category: Biomedical Science Authors: Ikeda K, Ikawa K, Yokoshige S, Yoshikawa S, Morikawa N Tags: Biomed Chromatogr Source Type: research

Study on the pharmacokinetics and metabolism of costunolide and dehydrocostus lactone in rats by HPLC-UV and UPLC-Q-TOF/MS.
Abstract A method based on high-performance liquid chromatography coupled with ultraviolet detection was developed for studying the pharmacokinetics of costunolide (Cos) and dehydrocostus lactone (Dehy) in rats after intravenous (i.v.) administration. Following i.v. administration, the maximum plasma concentrations of Cos and Dehy were observed to be 12.29 ± 1.47 and 5.79 ± 0.13 µg/mL, respectively. The bioavailability of Cos was larger than that of Dehy; however, the clearance and the volume of distribution of Dehy were much larger than those of Cos. An ultraperformance liquid chromatography/quadrupole ...
Source: Biomedical Chromatography : BMC - June 2, 2014 Category: Biomedical Science Authors: Peng Z, Wang Y, Gu X, Guo X, Yan C Tags: Biomed Chromatogr Source Type: research

Ribonucleoprotein Therapy in Alzheimer's Disease?
PMID: 24907158 [PubMed - as supplied by publisher] (Source: Aging)
Source: Aging - June 2, 2014 Category: Biomedical Science Authors: Yoon JH, Gorospe M Tags: Aging (Albany NY) Source Type: research

Original Hypothesis: Extracorporeal Shockwaves as a Homeostatic Autoimmune Restorative Treatment (HART) for Type 1 Diabetes Mellitus
(Source: Medical Hypotheses)
Source: Medical Hypotheses - June 2, 2014 Category: Biomedical Science Authors: Kenneth Craig, Cristina D’Agostino, Daniel Poratt, Marjorie Walker Source Type: research

Retraction
(Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - June 1, 2014 Category: Biomedical Science Tags: Retraction Source Type: research

Editorial Board
(Source: Metabolism - Clinical and Experimental)
Source: Metabolism - Clinical and Experimental - June 1, 2014 Category: Biomedical Science Source Type: research

Toc
(Source: Metabolism - Clinical and Experimental)
Source: Metabolism - Clinical and Experimental - June 1, 2014 Category: Biomedical Science Source Type: research

Masthead
(Source: Metabolism - Clinical and Experimental)
Source: Metabolism - Clinical and Experimental - June 1, 2014 Category: Biomedical Science Source Type: research

Retraction: “High‐level production of 3‐hydroxypropionatein Escherichia coli and Saccharomyces cerevisiae by introducing part of the 3‐hydroxypropionate/4‐hydroxybutyrate cycle from Metallosphaera sedula” by Z. Liu, Y. Wang, Z. Deng and T. Liu
(Source: Biotechnology and Bioengineering)
Source: Biotechnology and Bioengineering - June 1, 2014 Category: Biomedical Science Tags: Retraction Source Type: research

Imatinib Activates Pathological Hypertrophy by Altering Myocyte Calcium Regulation
ConclusionThese data show that imatinib can activate pathological hypertrophic signaling pathways by altering intracellular Ca2+ dynamics. This is likely a contributing mechanism for the adverse cardiac effects of imatinib. (Source: Clinical and Translational Science)
Source: Clinical and Translational Science - June 1, 2014 Category: Biomedical Science Authors: Larry A. Barr, Catherine A. Makarewich, Remus M. Berretta, Hui Gao, Constantine D. Troupes, Felix Woitek, Fabio Recchia, Hajime Kubo, Thomas Force, Steven R. Houser Tags: Article Source Type: research

Academic Medical Product Development: An Emerging Alliance of Technology Transfer Organizations and the CTSA
Abstract To bring the benefits of science more quickly to patient care, the NIH National Center Advancing Translational Sciences (NCATS) supports programs that enhance the development, testing, and implementation of new medical products and procedures. The NCATS clinical and translational science award (CTSA) program is central to that mission; creating an academic home for clinical and translational science and supporting those involved in the discovery and development of new health‐related inventions. The technology transfer Offices (TTO) of CTSA‐funded universities can be important partners in the development proces...
Source: Clinical and Translational Science - June 1, 2014 Category: Biomedical Science Authors: Lynn M. Rose, Maaike Everts, Caren Heller, Christine Burke, Nathaniel Hafer, Scott Steele Tags: Article Source Type: research

Clinical biobanks, from the world to china.
PMID: 24961861 [PubMed - in process] (Source: Biomedical and Environmental Sciences : BES)
Source: Biomedical and Environmental Sciences : BES - June 1, 2014 Category: Biomedical Science Authors: Ji X, Zhao XM, Jiang JJ, Yin L, Guo YC Tags: Biomed Environ Sci Source Type: research