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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 9.

Formation of ethyl ferulate from feruloylated oligosaccharide by transesterification of rice koji enzyme under sake mash conditions
Publication date: Available online 17 July 2015 Source:Journal of Bioscience and Bioengineering Author(s): Nobukazu Suzuki, Toshihiko Ito, Kai Hiroshima, Tetsuo Tokiwano, Katsumi Hashizume Formation of ethyl ferulate (EF) and ferulic acid (FA) under sake mash conditions was studied using feruloylated oligosaccharide (FO), prepared from rice grains, as the substrate for rice koji enzyme. EF and FA were produced from FO over six times faster than from alkyl ferulates however, under the same ethanol concentration, only small differences were observed between the EF/FA ratios when either FO or methyl ferulate were used ...
Source: Journal of Bioscience and Bioengineering - July 18, 2015 Category: Biomedical Science Source Type: research

Fucoidan from sea cucumber Cucumaria frondosa exhibits anti-hyperglycemic effects in insulin resistant mice via activating the PI3K/PKB pathway and GLUT4
Publication date: Available online 17 July 2015 Source:Journal of Bioscience and Bioengineering Author(s): Yiming Wang, Jingfeng Wang, Yanlei Zhao, Shiwei Hu, Di Shi, Changhu Xue The present study investigated the anti-hyperglycemic properties and mechanisms of fucoidan, isolated from Cucumaria frondosa (Cf-FUC), in insulin resistant mice. Male C57BL/6J mice were fed regular diet or high-fat/high-sucrose diet for 19 weeks. Model animals were dietary administrated either rosiglitazone (RSG, 1 mg/kg·bw), fucoidan (Cf-FUC, 80 mg/kg·bw) or their combinations. Results showed that Cf-FUC significantly reduced fasting...
Source: Journal of Bioscience and Bioengineering - July 18, 2015 Category: Biomedical Science Source Type: research

GLP-1R expression in the major salivary glands of rats
Conclusions This is the first time that GLP-1R expression has been detected in rat PG, SMG, and SLG. Our findings provide new insights into the effects of GLP-1 and GLP-1R in the major salivary glands of rats. Furthermore, our results indicate a new role for GLP-1 in salivary secretion, and potentially a new pathway for glucose metabolism. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - July 18, 2015 Category: Biomedical Science Source Type: research

The sonic hedgehog signaling pathway and the development of pharyngeal arch Derivatives in Haplochromis piceatus, a Lake Victoria cichlid
Conclusions These results suggest that the shh pathway is important for the development of pharyngeal arch derivatives such as teeth, premaxilla, operculum, and gill filaments in H. piceatus. (Source: Journal of Oral Biosciences)
Source: Journal of Oral Biosciences - July 18, 2015 Category: Biomedical Science Source Type: research

Systematic evaluation of matrix effect and cross talk free method for simultaneous determination of zolmitriptan and N-desmethyl zolmitriptan in human plasma: a sensitive LC-MS/MS method validation and its application to a clinical PK study.
This article is protected by copyright. All rights reserved. PMID: 26189757 [PubMed - as supplied by publisher] (Source: Biomedical Chromatography : BMC)
Source: Biomedical Chromatography : BMC - July 18, 2015 Category: Biomedical Science Authors: Patel B, Suhagiya BN, Jangid AG, Mistri HN, Desai N Tags: Biomed Chromatogr Source Type: research

Elevated serum prolactin levels as a marker of inflammatory arthritis in psoriasis vulgaris.
CONCLUSION: Our results showed that PRL serum levels are a marker of active arthritis in PsA and reflects systemic complication rather than local skin activity. PMID: 26175050 [PubMed - as supplied by publisher] (Source: Biomedical Papers of the Medical Faculty of the Univ Palacky Olomouc Czech Repub)
Source: Biomedical Papers of the Medical Faculty of the Univ Palacky Olomouc Czech Repub - July 17, 2015 Category: Biomedical Science Tags: Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub Source Type: research

Serum UCH-L1 as a Novel Biomarker to Predict Neuronal Apoptosis Following Deep Hypothermic Circulatory Arrest.
Conclusion: Serum UCH-L1, as an easy and quick measurable biomarker, can predict neural apoptosis induced by DHCA. The elevation in UCH-L1 concentration is consistent with the severity of neural apoptosis following DHCA. PMID: 26180514 [PubMed - as supplied by publisher] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - July 17, 2015 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Research Progress of Moyamoya Disease in Children.
Authors: Piao J, Wu W, Yang Z, Yu J Abstract During the onset of Moyamoya disease (MMD), progressive occlusion occurs at the end of the intracranial internal carotid artery, and compensatory net-like abnormal vessels develop in the skull base, generating the corresponding clinical symptoms. MMD can affect both children and adults, but MMD in pediatric patients exhibits distinct clinical features, and the treatment prognoses are different from adult patients. Children are the group at highest risk for MMD. In children, the disease mainly manifests as ischemia, while bleeding is the primary symptom in adults. The pat...
Source: International Journal of Medical Sciences - July 17, 2015 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Etomidate Anesthesia during ERCP Caused More Stable Haemodynamic Responses Compared with Propofol: A Randomized Clinical Trial.
Conclusions: Etomidate anesthesia during ERCP caused more stable haemodynamic responses compared with propofol. PMID: 26180512 [PubMed - as supplied by publisher] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - July 17, 2015 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Depressed Systemic Arterial Compliance is Associated with the Severity of Heart Failure Symptoms in Moderate-to-Severe Aortic Stenosis: a Cross-Sectional Retrospective Study.
CONCLUSIONS: Depressed SAC may enhance exercise intolerance irrespective of stenosis severity or left ventricular systolic function in moderate-to-severe AS. This finding supports the importance of non-valvular factors for symptomatic status in AS. PMID: 26180511 [PubMed - in process] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - July 17, 2015 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Cytological Kinetics of Periodontal Ligament in an Experimental Occlusal Trauma Model.
Authors: Takaya T, Mimura H, Matsuda S, Nakano K, Tsujigiwa H, Tomida M, Okafuji N, Fujii T, Kawakami T Abstract Using a model of experimental occlusal trauma in mice, we investigated cytological kinetics of periodontal ligament by means of histopathological, immunohistochemical, and photographical analysis methods. Periodontal ligament cells at furcation areas of molar teeth in the experimental group on day 4 showed a proliferation tendency of periodontal ligament cells. The cells with a round-shaped nucleus deeply stained the hematoxylin and increased within the day 4 specimens. Ki67 positive nuclei showed a prom...
Source: International Journal of Medical Sciences - July 17, 2015 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Investigation of Somatic NKX2-5 Mutations in Chinese Children with Congenital Heart Disease.
Authors: Zheng J, Li F, Liu J, Xu Z, Zhang H, Fu Q, Wang J, Sun K Abstract The purposes of this study are to investigate somatic NKX2-5 mutations in Chinese children with congenital heart disease (CHD) and assess the reliability of somatic mutation detection in formalin-fixed, paraffin-embedded (FFPE) tissues. The study cohort included frozen and FFPE cardiac tissues as well as blood samples from 85 Chinese children with CHD who had the cardiac operations. The right atrial appendage far from the diseased heart was used as normal control. Genomic DNA was isolated from cardiac tissues and blood samples using TIANamp ...
Source: International Journal of Medical Sciences - July 17, 2015 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

Compliance Index, a Marker of Peripheral Arterial Stiffness, may Predict Renal Function Decline in Patients with Chronic Kidney Disease.
Conclusions: Compliance index, CI-DVP, was significantly associated with renal function decline in patients with CKD. A higher CI-DVP may have independent prognostic value in slower renal function decline and better composite renal and cardiovascular outcomes in CKD patients. PMID: 26180508 [PubMed - as supplied by publisher] (Source: International Journal of Medical Sciences)
Source: International Journal of Medical Sciences - July 17, 2015 Category: Biomedical Science Tags: Int J Med Sci Source Type: research

C35 is overexpressed in colorectal cancer and is associated tumor invasion and metastasis.
Authors: Dong X, Huang Y, Kong L, Li J, Kou J, Yin L, Yang J Abstract The aim of this study was to investigate the expression of C35, an oncogene previously found in breast and prostate cancers, and its clinicopathological significance in colorectal cancer (CRC). Qualitative and quantitative detection of C35 mRNA expression was performed using reverse transcription-PCR (RT-PCR) and real-time PCR. C35 protein expression was determined using immunohistochemistry. C35 mRNA was detected in none of 10 normal colorectal tissue samples, 55 of 65 (84.6%) CRC tissue samples, and 43 of 55 (78.2%) adjacent non-cancerous tissu...
Source: BioScience Trends - July 17, 2015 Category: Biomedical Science Tags: Biosci Trends Source Type: research

Upregulation of Bcl-2 enhances secretion of growth factors by adipose-derived stem cells deprived of oxygen and glucose.
This study modified ADSCs with the Bcl-2 gene in order to increase the secretion of growth factors during oxygen-glucose deprivation (OGD). The phenotypes of human ADSCs that were passaged 4 times were analyzed using flow cytometry. Then, ADSCs were genetically modified with Bcl-2 and Bcl-2 gene transduction was verified with Western blotting. Proliferative capacity and multipotent differentiation properties were evaluated in Bcl-2-modified ADSCs. Secretion of vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and basic fibroblast growth factor (bFGF) was evaluated using an enzyme-linked immunosorbe...
Source: BioScience Trends - July 17, 2015 Category: Biomedical Science Tags: Biosci Trends Source Type: research

A cross-sectional study of leukopenia and thrombocytopenia among Chinese adults with newly diagnosed HIV/AIDS.
A cross-sectional study of leukopenia and thrombocytopenia among Chinese adults with newly diagnosed HIV/AIDS. Biosci Trends. 2015 Apr;9(2):91-6 Authors: Shen Y, Wang J, Wang Z, Shen J, Tangkai Qi, Song W, Tang Y, Liu L, Zhang R, Zeng Y, Lu H Abstract We conducted a cross-sectional study to determine the prevalence and risk factors of leukopenia and thrombocytopenia among Chinese adults with newly diagnosed HIV/AIDS. One thousand nine hundred and forty-eight newly diagnosed HIV-infected patients were enrolled between 2009 and 2010. Serum samples obtained from each individual were collected for complet...
Source: BioScience Trends - July 17, 2015 Category: Biomedical Science Tags: Biosci Trends Source Type: research

Developmental Origins of Health and Disease (DOHaD): Implications for health and nutritional issues among rural children in China.
Authors: Feng A, Wang L, Chen X, Liu X, Li L, Wang B, Luo H, Mo X, Tobe RG Abstract In China, with fast economic growth, health and nutrition status among the rural population has shown significant improvement in the past decades. On the other hand, burden of non-communicable diseases and prevalence of related risk factors such as overweight and obesity has also increased. Among rural children, the double burden of malnutrition and emerging overweight and obesity has been neglected so far. According to the theory of Developmental Origin of Health and Diseases (DOHaD), malnutrition, including both undernutrition (st...
Source: BioScience Trends - July 17, 2015 Category: Biomedical Science Tags: Biosci Trends Source Type: research

Title page/Editorial Board
(Source: Medical Hypotheses)
Source: Medical Hypotheses - July 17, 2015 Category: Biomedical Science Source Type: research

Twin Dipole Fano Resonances in Symmetric Three-Layered Plasmonic Nanocylinder
Abstract A unique metal-dielectric structure composed of symmetric multilayered nanocylinder is demonstrated to support unconventional superradiant and subradiant plasmon modes. Here, the twin distinctive dipole-dipole Fano resonances are observed in the structure within the visible spectrum by carefully selecting the geometrical parameters. The spectral features of these Fano resonances are controlled by changing the incident field polarization and parameters of the nanoparticle. Eventually, an RLC circuit model is proposed to reproduce the optical response of the nanostructure. (Source: Plasmonics)
Source: Plasmonics - July 17, 2015 Category: Biomedical Science Source Type: research

Theoretical Study of Phase-Interrogated Surface Plasmon Resonance Based on Optical Fiber Sensors with Metallic and Oxide Layers
Abstract This work reports the theoretical investigation of optical fiber surface plasmon resonance sensors incorporating an internal metallic layer of silver covered with an oxide layer. This research is supported by the application of an effective analytical model combining geometrical optics with the transfer matrix theory for stratified optical media. Different oxide materials like titanium dioxide, silicon dioxide, and aluminum oxide are considered aiming to achieve increased/enhanced sensitivity to refractive index variations of the external medium, particularly when addressing phase interrogation. It is sh...
Source: Plasmonics - July 17, 2015 Category: Biomedical Science Source Type: research

Nanoscale Control of Temperature Distribution Using a Plasmonic Trimer
Abstract A plasmonic trimer composed of three closely packed identical gold nanospheres for manipulating nanoscale temperature distribution is proposed. It is shown that heat can be unevenly distributed among the three particles, creating a high temperature gradient in the nanoscale space despite the strong thermalization effect. Moreover, the difference in temperature increment among the particles is sensitive to the polarization of incident light and can be continuously tuned. The dependence of the achievable temperature difference on the trimer parameters is also investigated. The ability of nanoscale selectiv...
Source: Plasmonics - July 17, 2015 Category: Biomedical Science Source Type: research

Quantum Mechanical Model for Plasmon Excitations in Nanostructures
Abstract In this work, we develop a theoretical quantum mechanical model for describing the plasmon excitations in nanostructures. This model is based on the electronic density according to the Density Functional Theory (DFT). We derive an expression for finding an approximation to the dispersion relation in a nanostructure from the complete Hamiltonian of the system. We apply this expression to an aluminium nanoring showing how great, and not negligible, the ionic-interaction term is. (Source: Plasmonics)
Source: Plasmonics - July 17, 2015 Category: Biomedical Science Source Type: research

A Visual Colorimetric Probe for Naked-Eye Detection of Pamidronate Disodium in Human Plasma Based on Aggregation of Citrate-Capped Gold Nanoparticles
Abstract Herein, a sensitive, simple, and rapid colorimetric assay for the detection of pamidronate disodium was developed based on the aggregation of citrate-capped gold nanoparticles (AuNPs). This was exploited from the affinity of electron-rich atoms toward the surface of AuNPs, resulting in the aggregation of AuNPs through intermolecular hydrogen bonding interactions. As a result of aggregation under optimum conditions ([NaCl] = 25 mM, [AuNPs] = 3.3 nM and pH 7.0), the color of AuNPs was changed from red to blue and the plasmon band of AuNPs around 520 was decreased along with the formation of a new...
Source: Plasmonics - July 17, 2015 Category: Biomedical Science Source Type: research

Reserve stem cells: Differentiated cells reprogram to fuel repair, metaplasia, and neoplasia in the adult gastrointestinal tract.
Abstract It has long been known that differentiated cells can switch fates, especially in vitro, but only recently has there been a critical mass of publications describing the mechanisms adult, postmitotic cells use in vivo to reverse their differentiation state. We propose that this sort of cellular reprogramming is a fundamental cellular process akin to apoptosis or mitosis. Because reprogramming can invoke regenerative cells from mature cells, it is critical to the long-term maintenance of tissues like the pancreas, which encounter large insults during adulthood but lack constitutively active adult stem cells t...
Source: Science Signaling - July 17, 2015 Category: Biomedical Science Authors: Mills JC, Sansom OJ Tags: Sci Signal Source Type: research

The interaction of heparan sulfate proteoglycans with endothelial transglutaminase-2 limits VEGF165-induced angiogenesis.
Abstract Sprouting angiogenesis is stimulated by vascular endothelial growth factor (VEGF165) that is localized in the extracellular matrix (ECM) and binds to heparan sulfate (HS)-bearing proteins known as heparan sulfate proteoglycans (HSPGs). VEGF165 presentation by HSPGs enhances VEGF receptor-2 (VEGFR2) signaling. We investigated the effect of TG2, which binds to HSPGs, on the interaction between VEGF165 and HS and angiogenesis. Mice with tg2 deficiency showed transiently enhanced retina vessel formation and increased vascularization of VEGF165-containing Matrigel implants. In addition, endothelial cells in whi...
Source: Science Signaling - July 17, 2015 Category: Biomedical Science Authors: Beckouche N, Bignon M, Lelarge V, Mathivet T, Pichol-Thievend C, Berndt S, Hardouin J, Garand M, Ardidie-Robouant C, Barret A, Melino G, Lortat-Jacob H, Muller L, Monnot C, Germain S Tags: Sci Signal Source Type: research

Distinct single-cell signaling characteristics are conferred by the MyD88 and TRIF pathways during TLR4 activation.
Abstract Toll-like receptors (TLRs) recognize specific pathogen-associated molecular patterns and initiate innate immune responses through signaling pathways that depend on the adaptor proteins MyD88 (myeloid differentiation marker 88) or TRIF (TIR domain-containing adaptor protein-inducing interferon-β). TLR4, in particular, uses both adaptor proteins to activate the transcription factor nuclear factor κB (NF-κB); however, the specificity and redundancy of these two pathways remain to be elucidated. We developed a mathematical model to show how each pathway encodes distinct dynamical features of NF-κB activity...
Source: Science Signaling - July 17, 2015 Category: Biomedical Science Authors: Cheng Z, Taylor B, Ourthiague DR, Hoffmann A Tags: Sci Signal Source Type: research

The rise in computational systems biology approaches for understanding NF-κB signaling dynamics.
Abstract A study by Cheng et al. in this issue of Science Signaling highlights the distinct single-cell signaling characteristics conferred by pathways mediated by the adaptor proteins MyD88 and TRIF in the TLR4-dependent activation of the transcription factor nuclear factor κB (NF-κB). PMID: 26175491 [PubMed - in process] (Source: Science Signaling)
Source: Science Signaling - July 17, 2015 Category: Biomedical Science Authors: Williams RA, Timmis J, Qwarnstrom EE Tags: Sci Signal Source Type: research

Mammalian Bcnt/Cfdp1, a potential epigenetic factor characterized by an acidic stretch in the disordered N-terminal and Ser250 phosphorylation in the conserved C-terminal regions.
Abstract The BCNT (Bucentaur) superfamily is classified by an uncharacteristic conserved sequence of ∼80 amino acids (aa) at the C-terminus, BCNT-C (the conserved C-terminal region of Bcnt/Cfdp1). Whereas the yeast Swc5 and Drosophila Yeti homologues play crucial roles in chromatin remodelling organization, mammalian Bcnt/Cfdp1 (craniofacial developmental protein 1) remains poorly understood. The protein, which lacks cysteine, is largely disordered and comprises an acidic N-terminal region, a lysine/glutamic acid/proline-rich 40 aa sequence and BCNT-C. It shows complex mobility on SDS/PAGE at ∼50 kDa, whereas ...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Iwashita S, Suzuki T, Yasuda T, Nakashima K, Sakamoto T, Kohno T, Takahashi I, Kobayashi T, Ohno-Iwashita Y, Imajoh-Ohmi S, Song SY, Dohmae N Tags: Biosci Rep Source Type: research

Oxidative stress induces early-onset apoptosis of vascular smooth muscle cells and neointima formation in response to injury.
Abstract The present study dissects the mechanisms underlying the rapid onset of apoptosis that precedes post injury vascular remodelling. Using the rat balloon injury model, we demonstrated that a significant number of arterial vascular smooth muscle cells (VSMC) undergo apoptosis at 90 min after the procedure. This apoptotic wave caused significant loss in media cellularity (>90%) over the next 3 h and was accompanied by a marked accumulation of oxidative stress by-products in the vascular wall. Early apoptotic VSMC were rich in p38 mitogen-activated protein kinase (MAPK) and the transcription factor c-Jun a...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Gomez C, Martinez L, Mesa A, Duque JC, Escobar LA, Pham SM, Vazquez-Padron RI Tags: Biosci Rep Source Type: research

A biophysical study on molecular physiology of the uncoupling proteins of the central nervous system.
Abstract Mitochondrial inner membrane uncoupling proteins (UCPs) facilitate transmembrane (TM) proton flux and consequently reduce the membrane potential and ATP production. It has been proposed that the three neuronal human UCPs (UCP2, UCP4 and UCP5) in the central nervous system (CNS) play significant roles in reducing cellular oxidative stress. However, the structure and ion transport mechanism of these proteins remain relatively unexplored. Recently, we reported a novel expression system for obtaining functionally folded UCP1 in bacterial membranes and applied this system to obtain highly pure neuronal UCPs in...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Hoang T, Kuljanin M, Smith MD, Jelokhani-Niaraki M Tags: Biosci Rep Source Type: research

Antibody-drug conjugates as novel anti-cancer chemotherapeutics.
Abstract Over the past couple of decades, antibody-drug conjugates (ADCs) have revolutionized the field of cancer chemotherapy. Unlike conventional treatments that damage healthy tissues upon dose escalation, ADCs utilize monoclonal antibodies (mAbs) to specifically bind tumour-associated target antigens and deliver a highly potent cytotoxic agent. The synergistic combination of mAbs conjugated to small-molecule chemotherapeutics, via a stable linker, has given rise to an extremely efficacious class of anti-cancer drugs with an already large and rapidly growing clinical pipeline. The primary objective of this paper...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Peters C, Brown S Tags: Biosci Rep Source Type: research

Functional role of histone variant Htz1 in the stress response to oleate in Saccharomyces cerevisiae.
Abstract Chromatin structure is implicated in regulating gene transcription in stress response. Transcription factors, transferases and deacetylases, such as multicopy suppressor of SNF1 protein 2 (Msn2), SET domain-containing protein 1 (Set1) and sucrose NonFermenting protein 1 (Snf1), have been identified as key regulators in stress response. In the present study, we reported the dynamics of nucleosome occupancy, Histone Two A Z1 (Htz1) deposition and histone H3 lysine 4 dimethylation (H3K4me2) and histone H3 lysine 79 trimethylation (H3K79me3) in Saccharomyces cerevisiae under oleate stress. Our results indicate...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Liu H, Li G, Liu L, Wan Y Tags: Biosci Rep Source Type: research

Specific GFP-binding artificial proteins (αRep): a new tool for in vitro to live cell applications.
Abstract A family of artificial proteins, named αRep, based on a natural family of helical repeat was previously designed. αRep members are efficiently expressed, folded and extremely stable proteins. A large αRep library was constructed creating proteins with a randomized interaction surface. In the present study, we show that the αRep library is an efficient source of tailor-made specific proteins with direct applications in biochemistry and cell biology. From this library, we selected by phage display αRep binders with nanomolar dissociation constants against the GFP. The structures of two independent αRep...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Chevrel A, Urvoas A, de la Sierra-Gallay IL, Aumont-Nicaise M, Moutel S, Desmadril M, Perez F, Gautreau A, van Tilbeurgh H, Minard P, Valerio-Lepiniec M Tags: Biosci Rep Source Type: research

High homocysteine induces betaine depletion.
Abstract Betaine is the substrate of the liver- and kidney-specific betaine-homocysteine (Hcy) methyltransferase (BHMT), an alternate pathway for Hcy remethylation. We hypothesized that BHMT is a major pathway for homocysteine removal in cases of hyperhomocysteinaemia (HHcy). Therefore, we measured betaine in plasma and tissues from patients and animal models of HHcy of genetic and acquired cause. Plasma was collected from patients presenting HHcy without any Hcy interfering treatment. Plasma and tissues were collected from rat models of HHcy induced by diet and from a mouse model of cystathionine β-synthase (CBS)...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Imbard A, Benoist JF, Esse R, Gupta S, Lebon S, de Vriese AS, de Baulny HO, Kruger W, Schiff M, Blom HJ Tags: Biosci Rep Source Type: research

The regulation of mitochondrial transcription factor A (Tfam) expression during skeletal muscle cell differentiation.
Abstract The ATP demand required for muscle development is accommodated by elevations in mitochondrial biogenesis, through the co-ordinated activities of the nuclear and mitochondrial genomes. The most important transcriptional activator of the mitochondrial genome is mitochondrial transcription factor A (Tfam); however, the regulation of Tfam expression during muscle differentiation is not known. Thus, we measured Tfam mRNA levels, mRNA stability, protein expression and localization and Tfam transcription during the progression of muscle differentiation. Parallel 2-fold increases in Tfam protein and mRNA were obse...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Collu-Marchese M, Shuen M, Pauly M, Saleem A, Hood DA Tags: Biosci Rep Source Type: research

SNAP23 is selectively expressed in airway secretory cells and mediates baseline and stimulated mucin secretion.
Abstract Airway mucin secretion is important pathophysiologically and as a model of polarized epithelial regulated exocytosis. We find the trafficking protein, SNAP23 (23-kDa paralogue of synaptosome-associated protein of 25 kDa), selectively expressed in secretory cells compared with ciliated and basal cells of airway epithelium by immunohistochemistry and FACS, suggesting that SNAP23 functions in regulated but not constitutive epithelial secretion. Heterozygous SNAP23 deletant mutant mice show spontaneous accumulation of intracellular mucin, indicating a defect in baseline secretion. However mucins are released ...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Ren B, Azzegagh Z, Jaramillo AM, Zhu Y, Pardo-Saganta A, Bagirzadeh R, Flores JR, Han W, Tang YJ, Tu J, Alanis DM, Evans CM, Guindani M, Roche PA, Rajagopal J, Chen J, Davis CW, Tuvim MJ, Dickey BF Tags: Biosci Rep Source Type: research

Fibrinogen-like protein 2 gene silencing inhibits cardiomyocytes apoptosis, improves heart function of streptozotocin-induced diabetes rats and the molecular mechanism involved.
In conclusion, Fgl2 is a potent target to treat DCM. PMID: 26182381 [PubMed - as supplied by publisher] (Source: Bioscience Reports)
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Zhenzhong Z, Yafa Y, Jin L Tags: Biosci Rep Source Type: research

Modulation of the extracellular matrix patterning of thrombospondins by actin dynamics and thrombospondin oligomer state.
Abstract Thrombospondins (TSPs) are evolutionarily-conserved, secreted glycoproteins that interact with cell surfaces and extracellular matrix (ECM) and have complex roles in cell interactions. Unlike the structural components of the ECM that form networks or fibrils, TSPs are deposited into ECM as arrays of nanoscale puncta. The cellular and molecular mechanisms for the patterning of TSPs in ECM are poorly understood. In the present study, we investigated whether the mechanisms of TSP patterning in cell-derived ECM involves actin cytoskeletal pathways or TSP oligomer state. From tests of a suite of pharmacological...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Hellewell AL, Gong X, Schärich K, Christofidou ED, Adams JC Tags: Biosci Rep Source Type: research

PGC-1α overexpression suppresses blood pressure elevation in DOCA-salt hypertensive mice.
Abstract Increasing evidences have accumulated that endothelial dysfunction is involved in the pathogenesis of hypertension. Peroxisome proliferator-activated receptor γ (PPARγ) coactivator-1α (PGC-1α) has been identified as an essential factor that protects against endothelial dysfunction in vascular pathologies. However, the functional role of PGC-1α in hypertension is not well understood. Using an adenovirus infection model, we tested the hypothesis that PGC-1α overexpression retards the progression of hypertension in deoxycorticosterone acetate (DOCA)-salt mice model through preservation of the function o...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Zhao Q, Zhang J, Wang H Tags: Biosci Rep Source Type: research

Cardioprotective effects and pharmacokinetic properties of a controlled release formulation of a novel hydrogen sulfide donor in rats with acute myocardial infarction.
Abstract We previously reported that S-propargyl-cysteine (SPRC) exerts cardioprotective effects by elevating H2S levels via the CSE/H2S pathway. In the present study, we investigated the cardioprotective effects and pharmacokinetic properties of a controlled release formulation of SPRC (CR-SPRC) in an in vivo rat model of myocardial infarction (MI). Rats were randomly assigned to seven groups that were pre-treated with CR-SPRC daily for 7 days prior to ligation of the left anterior descending coronary artery to induce MI. Cardiac function and infarct size were determined after MI, and we examined the activity of...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Tran BH, Huang C, Zhang Q, Liu X, Lin S, Liu H, Wang S, Zhu YZ Tags: Biosci Rep Source Type: research

Cysteine-specific ubiquitination protects the peroxisomal import receptor Pex5p against proteasomal degradation.
Abstract Peroxisomal matrix protein import is mediated by dynamic import receptors, which cycle between the peroxisomal membrane and the cytosol. Proteins with a type 1 peroxisomal targeting signal (PTS1) are bound by the import receptor Pex5p in the cytosol and guided to the peroxisomal membrane. After cargo translocation into the peroxisomal matrix, the receptor is released from the membrane back to the cytosol in an ATP-dependent manner by the AAA-type ATPases Pex1p and Pex6p. These mechanoenzymes recognize ubiquitinated Pex5p-species as substrates for membrane extraction. The PTS1-receptor is either polyubiqui...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Schwartzkopff B, Platta HW, Hasan S, Girzalsky W, Erdmann R Tags: Biosci Rep Source Type: research

Cadmium toxicity induces ER stress and apoptosis via impairing energy homoeostasis in cardiomyocytes.
Abstract Cadmium, a highly toxic environmental pollutant, is reported to induce toxicity and apoptosis in multiple organs and cells, all possibly contributing to apoptosis in certain pathophysiologic situations. Previous studies have described that cadmium toxicity induces biochemical and physiological changes in the heart and finally leads to cardiac dysfunctions, such as decreasing contractile tension, rate of tension development, heart rate, coronary flow rate and atrioventricular node conductivity. Although many progresses have been made, the mechanism responsible for cadmium-induced cellular alternations and c...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Chen CY, Zhang SL, Liu ZY, Tian Y, Sun Q Tags: Biosci Rep Source Type: research

Reduction in mdx mouse muscle degeneration by low-intensity endurance exercise: a proteomic analysis in quadriceps muscle of exercised compared with sedentary mdx mice.
Abstract In our recent study was shown a significant recovery of damaged skeletal muscle of mice with X-linked muscular dystrophy (mdx) following low-intensity endurance exercise, probably by reducing the degeneration of dystrophic muscle. Consequently, in the present work, we aimed to identify proteins involved in the observed reduction in degenerating fibres. To this end, we used proteomic analysis to evaluate changes in the protein profile of quadriceps dystrophic muscles of exercised compared with sedentary mdx mice. Four protein spots were found to be significantly changed and were identified as three isoforms...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Fontana S, Schillaci O, Frinchi M, Giallombardo M, Morici G, Liberto VD, Alessandro R, De Leo G, Perciavalle V, Belluardo N, Mudò G Tags: Biosci Rep Source Type: research

Does the liposuction method influence the phenotypic characteristic of human adipose-derived stem cells?
Abstract Adipose-derived stem cells (ASCs) possess a high differentiation and proliferation potential. However, the phenotypic characterization of ASCs is still difficult. Until now, there is no extensive analysis of ASCs markers depending on different liposuction methods. Therefore, the aim of the present study was to analyse 242 surface markers and determine the differences in the phenotypic pattern between ASCs obtained during mechanical and ultrasound-assisted liposuction. ASCs were isolated from healthy donors, due to mechanical and ultrasound-assisted liposuction and cultured in standard medium to the second ...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Bajek A, Gurtowska N, Gackowska L, Kubiszewska I, Bodnar M, Marszałek A, Januszewski R, Michalkiewicz J, Drewa T Tags: Biosci Rep Source Type: research

Diagnostic model of saliva peptide finger print analysis of oral squamous cell carcinoma patients using weak cation exchange magnetic beads.
Abstract Saliva diagnostics utilizing nanotechnology and molecular technologies to detect oral squamous cell carcinoma (OSCC) has become an attractive field of study. However, no specific methods have been established. To refine the diagnostic power of saliva peptide fingerprints for the early detection of OSCC, we screened the expression spectrum of salivary peptides in 40 T1 stage OSCC patients (and healthy controls) using MALDI-TOF-MS combined with magnetic beads. Fifty proteins showed significantly different expression levels in the OSCC samples (P<0.05). Potential biomarkers were also predicted. The novel d...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Jiang WP, Wang Z, Xu LX, Peng X, Chen F Tags: Biosci Rep Source Type: research

Selective activators of protein phosphatase 5 target the auto-inhibitory mechanism.
Abstract Protein phosphatase 5 (PP5) is an evolutionary conserved serine/threonine phosphatase. Its dephosphorylation activity modulates a diverse set of cellular factors including protein kinases and the microtubule-associated tau protein involved in neurodegenerative disorders. It is auto-regulated by its heat-shock protein (Hsp90)-interacting tetratricopeptide repeat (TPR) domain and its C-terminal α-helix. In the present study, we report the identification of five specific PP5 activators [PP5 small-molecule activators (P5SAs)] that enhance the phosphatase activity up to 8-fold. The compounds are allosteric mod...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Haslbeck V, Drazic A, Eckl JM, Alte F, Helmuth M, Popowicz G, Schmidt W, Braun F, Weiwad M, Fischer G, Gemmecker G, Sattler M, Striggow F, Groll M, Richter K Tags: Biosci Rep Source Type: research

Interaction of chromatin with a histone H1 containing swapped N- and C-terminal domains.
Abstract Although the details of the structural involvement of histone H1 in the organization of the nucleosome are quite well understood, the sequential events involved in the recognition of its binding site are not as well known. We have used a recombinant human histone H1 (H1.1) in which the N- and C-terminal domains (NTD/CTD) have been swapped and we have reconstituted it on to a 208-bp nucleosome. We have shown that the swapped version of the protein is still able to bind to nucleosomes through its structurally folded wing helix domain (WHD); however, analytical ultracentrifuge analysis demonstrates its abili...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Hutchinson JB, Cheema MS, Wang J, Missiaen K, Finn R, Gonzalez Romero R, Th'ng JP, Hendzel M, Ausió J Tags: Biosci Rep Source Type: research

3D spheroid cultures improve the metabolic gene expression profiles of HepaRG cells.
Abstract 3D (three-dimensional) cultures are considered to be an effective method for toxicological studies; however, little evidence has been reported whether 3D cultures have an impact on hepatocellular physiology regarding lipid or glucose metabolism. In the present study, we conducted physiological characterization of hepatoma cell lines HepG2 and HepaRG cells cultured in 3D conditions using a hanging drop method to verify the effect of culture environment on cellular responses. Apo (Apolipoprotein)B as well as albumin secretion was augmented by 3D cultures. Expression of genes related to not only drug, but als...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Takahashi Y, Hori Y, Yamamoto T, Urashima T, Ohara Y, Tanaka H Tags: Biosci Rep Source Type: research

Loss of protein targeting to glycogen sensitizes human hepatocellular carcinoma cells towards glucose deprivation mediated oxidative stress and cell death.
Abstract Protein targeting to glycogen (PTG) is a ubiquitously expressed scaffolding protein that critically regulates glycogen levels in many tissues, including the liver, muscle and brain. However, its importance in transformed cells has yet to be explored in detail. Since recent studies have demonstrated an important role for glycogen metabolism in cancer cells, we decided to assess the effect of PTG levels on the ability of human hepatocellular carcinoma (HepG2) cells to respond to metabolic stress. Although PTG expression did not significantly affect the proliferation of HepG2 cells under normal culture condit...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Yang R, Zhang M, Gustafson AR, Wang E, Cole MP, Tooley CE, Cheng A Tags: Biosci Rep Source Type: research

Human exonuclease 1 (EXO1) activity characterization and its function on flap structures.
Abstract Human exonuclease 1 (EXO1) is involved in multiple DNA metabolism processes, including DNA repair and replication. Most of the fundamental roles of EXO1 have been described in yeast. Here, we report a biochemical characterization of human full-length EXO1. Prior to assay EXO1 on different DNA flap structures, we determined factors essential for the thermodynamic stability of EXO1. We show that enzymatic activity and stability of EXO1 on DNA is modulated by temperature. By characterization of EXO1 flap activity using various DNA flap substrates, we show that EXO1 has a strong capacity for degrading double s...
Source: Bioscience Reports - July 17, 2015 Category: Biomedical Science Authors: Keijzers G, Bohr VA, Rasmussen LJ Tags: Biosci Rep Source Type: research