Biotechnology Research 
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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 13.
Recombinant Production of Crab Antimicrobial Protein Scygonadin Expressed as Thioredoxin and SUMO Fusions in Escherichia coli.
In this study, I developed a protocol for producing recombinant scygonadin in Escherichia coli. The target protein was expressed as both thioredoxin and SUMO fusions, and released by TEV and SUMO protease-mediated cleavages, respectively. In either case, the liberated scygonadin was separated from its carrier using a HisTrap HP column. From thioredoxin and SUMO fusion constructs, 32.7 and 29.2 mg target protein per liter of culture was obtained, respectively. The described protocol provides an effective means for producing scygonadin in relatively large quantities, which facilities its further characterization.
PMID: ...
Source: Applied Biochemistry and Biotechnology - January 24, 2013 Category: Biochemistry Authors: Li Y Tags: Appl Biochem Biotechnol Source Type: research
Prediction of Optimal pH in Hydrolytic Reaction of Beta-glucosidase.
In this study, 23 features selected from 540 plus features of individual amino acid as well as a feature combined whole protein information were screened as independents in a 20-1 feedforward backpropagation neural network for predicting optimal pH in beta-glucosidase's hydrolytic reaction because this enzyme drew attention recently due to its role in biofuel industry. The results show that 11 features can be used as independents for the prediction, while the feature of amino acid distribution probability works better than the rest independents for the prediction. Our study paves a way to predict the optimal reaction param...
Source: Applied Biochemistry and Biotechnology - January 24, 2013 Category: Biochemistry Authors: Yan S, Wu G Tags: Appl Biochem Biotechnol Source Type: research
Linking Performance with Microbial Community Characteristics in an Anaerobic Baffled Reactor.
Abstract
The performance and microbial community characteristics of a laboratory scale anaerobic baffled reactor (ABR) with four compartments (C1-C4) treating sugar refinery wastewater were investigated. The COD removal was 94.8 % with a CH(4) yield of 0.21 L g(-1) COD(removed) at total organic loading rate (OLR) of 5.33 kg COD/m(-3) day(-1). Fermentative bacteria were dominant in C1 and C2, while syntrophic acetogens and methanogens were dominant in C3 and C4. Some acid-tolerant methanogens were enriched in acidogenic phase. The present of the acid-tolerant methanogens could improve the efficiency and stabi...
Source: Applied Biochemistry and Biotechnology - January 24, 2013 Category: Biochemistry Authors: Ban Q, Li J, Zhang L, Jha AK, Nies L Tags: Appl Biochem Biotechnol Source Type: research
Secretory Phospholipase A(2) in Dromedary Tears: a Host Defense Against Staphylococci and Other Gram-Positive Bacteria.
In this report, we have analyzed the bactericidal activity of dromedary tears and the subsequently purified sPLA(2) on several Gram-positive bacteria. Our results showed that the sPLA(2) displays a potent bactericidal activity against all the tested bacteria particularly against the Staphylococcus strains when tested in the ionic environment of tears. There is a synergic action of the sPLA(2) with lysozyme when added to the bacteria culture prior to sPLA(2). Interestingly, lysozyme purified from dromedary tears showed a significant bactericidal activity against Listeria monocytogene and Staphylococcus epidermidis, whereas ...
Source: Applied Biochemistry and Biotechnology - January 24, 2013 Category: Biochemistry Authors: Ben Bacha A, Abid I Tags: Appl Biochem Biotechnol Source Type: research
Enhancement of Streptomyces transglutaminase activity and pro-peptide cleavage efficiency by introducing linker peptide in the C-terminus of the pro-peptide.
In this study, we developed a new approach to improve the properties of TGase based on pro-peptide engineering. When the α-helix(37G-42S) in pro-peptide was substituted with three glycines and three alanines respectively, the mutants exhibited higher specific activity and the efficiency of pro-peptide cleavage was enhanced. To further improve the properties of TGase, relevant mutations were constructed by introducing linker peptides in the C-terminus of the pro-peptide. Mutants with GS (GGGGS) and PT (PTPPTTPT) linker peptide exhibited 1.28 fold and 1.5 fold higher specific activity than the wild-type enzyme, respectively...
Source: Journal of Industrial Microbiology and Biotechnology - January 24, 2013 Category: Microbiology Authors: Chen K, Liu S, Wang G, Zhang D, Du G, Chen J, Shi Z Tags: J Ind Microbiol Biotechnol Source Type: research
Construction of lactose-consuming Saccharomyces cerevisiae for lactose fermentation into ethanol fuel.
Abstract
Two lactose-consuming diploid Saccharomyces cerevisiae strains, AY-51024A and AY-51024M, were constructed by expressing the LAC4 and LAC12 genes of Kluyveromyces marxianus in the host strain AY-5. In AY-51024A, both genes were targeted to the ATH1 and NTH1 gene-encoding regions to abolish the activity of acid/neutral trehalase. In AY-51024M, both genes were respectively integrated into the MIG1 and NTH1 gene-encoding regions to relieve glucose repression. Physiologic studies of the two transformants under anaerobic cultivations in glucose and galactose media indicated that the expression of both LAC genes ...
Source: Journal of Industrial Microbiology and Biotechnology - January 24, 2013 Category: Microbiology Authors: Zou J, Guo X, Shen T, Dong J, Zhang C, Xiao D Tags: J Ind Microbiol Biotechnol Source Type: research
Integration in Bioanalysis: Technologies for Point-of-Care Testing.
This article gives a brief overview of recent achievements and future prospects.
PMID: 23344629 [PubMed - as supplied by publisher]
Source: Advances in Biochemical Engineering Biotechnology - January 24, 2013 Category: Biotechnology Authors: Bier FF, Schumacher S Tags: Adv Biochem Eng Biotechnol Source Type: research
Aerobic transformation of zinc into metal sulfide by photosynthetic microorganisms.
Abstract
Industrial activity over the last two centuries has increased heavy metal contamination worldwide, leading to greater human exposure. Zinc is particularly common in industrial effluents and although an essential nutrient, it is highly toxic at elevated concentrations. Photoautotrophic microbes hold promise for heavy metal bioremediation applications because of their ease of culture and their ability to produce sulfide through metabolic processes that in turn are known to complex with the metal ion, Hg(II). The green alga Chlamydomonas reinhardtii, the red alga Cyanidioschyzon merolae, and the cyanobacteriu...
Source: Applied Microbiology and Biotechnology - January 24, 2013 Category: Microbiology Authors: Edwards CD, Beatty JC, Loiselle JB, Vlassov KA, Lefebvre DD Tags: Appl Microbiol Biotechnol Source Type: research
Comparative functional genomics to reveal the molecular basis of phenotypic diversities and guide the genetic breeding of industrial yeast strains.
Abstract
An understanding of the genetic basis underlying the phenotypic variations of yeast strains would guide the breeding of this useful microorganism. Here, comparative functional genomics (CFG) of two bioethanol Saccharomyces cerevisiae strains (YJS329 and ZK2) with different stress tolerances and ethanol fermentation performances were performed. Our analysis indicated that different patterns of gene expression in the central carbon metabolism, antioxidative factors, and membrane compositions of these two strains are the main contributors to their various traits. Some of the differently expressed genes were d...
Source: Applied Microbiology and Biotechnology - January 24, 2013 Category: Microbiology Authors: Zheng DQ, Liu TZ, Chen J, Zhang K, Li O, Zhu L, Zhao YH, Wu XC, Wang PM Tags: Appl Microbiol Biotechnol Source Type: research
Biodiversity of Actinomycetes Associated with Caribbean Sponges and Their Potential for Natural Product Discovery.
In this study, 16 different species of Caribbean sponges were collected from four different locations in the coastal waters off Puerto Rico in order to examine diversity and bioactive metabolite production of marine actinomycetes in Caribbean sponges. Sediments were also collected from each location, in order to compare actinomycete communities between these two types of samples. A total of 180 actinomycetes were isolated and identified based on 16S rRNA gene analysis. Phylogenetic analysis revealed the presence of at least 14 new phylotypes belonging to the genera Micromonospora, Verruscosispora, Streptomyces, Salinospora...
Source: Marine Biotechnology - January 24, 2013 Category: Biotechnology Authors: Vicente J, Stewart A, Song B, Hill RT, Wright JL Tags: Mar Biotechnol (NY) Source Type: research
Toxin–antitoxin systems are ubiquitous and versatile modulators of prokaryotic cell fate
Abstract
Toxin–antitoxin (TA) systems are small genetic elements found on plasmids or chromosomes of countless bacteria, archaea, and possibly also unicellular fungi. Under normal growth conditions, the activity of the toxin protein or its translation is counteracted by an antitoxin protein or noncoding RNA. Five types of TA systems have been proposed that differ markedly in their genetic architectures and modes of activity control. Subtle regulatory properties, frequently responsive to environmental cues, impact the behavior of TA systems. Typically, stress conditions result in the degradation or depletion of the antito...
Source: FEMS Microbiology Letters - January 24, 2013 Category: Microbiology Authors: Christopher F. Schuster, Ralph Bertram Tags: MiniReview Source Type: research
Laboratory capacity for diagnosis of foot-and-mouth disease in Eastern Africa: implications for the progressive control pathway
Conclusions:
This study indicates that FMD diagnostic capacity in Eastern Africa is still inadequate and largely depends on antigen and antibody ELISAs techniques undertaken by the NRLs. Hence, for the region to progress on the PCP-FMD, there is need to: implement regional control measures, improve the serological diagnostic test performance and laboratory capacity of the NRLs (including training of personnel as well as upgrading of equipment and methods, especially strengthening the molecular diagnostic capacity), and to establish a regional reference laboratory to enforce QMS and characterization of FMD virus containing samples.
Source: BMC Veterinary Research - Latest articles - January 24, 2013 Category: Veterinary Research Authors: Alice NamatovuSabenzia WekesaKirsten TjørnehøjMoses DhikusookaVincent MuwanikaHans SiegsmundChrisostom Ayebazibwe Source Type: research
Repressible promoters -- A novel tool to generate conditional mutants in Pichia pastoris
Conclusion:
Our results show that all of the novel promoters can be used for repression of genes in liquid culture. We also came to the conclusion that the choice of the repressible promoter is of particular importance. For a successful repression experiment it is crucial that the native promoter of a gene and the repressible promoter in its non-repressed state are of similar strength.
Source: Microbial Cell Factories - January 24, 2013 Category: Microbiology Authors: Marizela DelicDiethard MattanovichBrigitte Gasser Source Type: research
Slideshow: Mission Bay, UCSF come of age
Life sciences researchers, California and San Francisco politicians and biotech companies applauded the 10th anniversary of the city's Mission Bay life sciences hub Wednesday.
See a slideshow of Wednesday's 10th-anniversary celebration. --->
In Genentech Hall -- a building occupied by the University of California, San Francisco, in January 2003 --speakers highlighted how the roughly 300-acre, onetime railyard and warehouse district has become a research enclave housing three Nobel Prize winners,…
Source: bizjournals.com Health Care:Hospitals headlines - January 23, 2013 Category: Hospital Management Authors: Ron Leuty Source Type: research
O’Malley floats $3 million tax credit pool to bolster cyber security
Maryland is looking to build on the success of a biotechnology tax credit to bolster another industry here — cyber security.
Gov. Martin O’Malley proposed in his fiscal 2014 budget a new cyber security tax credit that would set aside $3 million to encourage cyber security companies to expand or set up shop in Maryland.
“The impetus for it came from the fact that we regularly talk with not only cyber security companies but also investors in that space, some who are just discovering Maryland,”…
Source: bizjournals.com Health Care:Hospitals headlines - January 23, 2013 Category: Hospital Management Authors: Sarah Gantz Source Type: research
Genentech's Avastin approved for wider use in colorectal cancer
Genentech Inc.'s top-selling cancer drug Avastin won approval from U.S. regulators Wednesday to expand its use for metastatic colorectal cancer patients.
The Food and Drug Administration's decision allows Avastin, a monoclonal antibody that crimps the blood vessels that feed oxygen, blood and other nutrients to tumors, to be used by metastatic colorectal cancer patients whose cancer progressed when taking Avastin as their first line of cancer-fighting drugs.
Typically, doctors switch patients off…
Source: bizjournals.com Health Care:Biotechnology headlines - January 23, 2013 Category: Biotechnology Authors: Ron Leuty Source Type: research
WPS subsidiary Arise adds Aurora to network
Arise Health Plan, a subsidiary of WPS Health Insurance in Madison, said it reached an agreement with Aurora Health Care to include Aurora in Arise’s network.
WPS Health Insurance also will add Milwaukee-based Aurora Health Care to its network sometime this year, but no official date has been set, WPS said Wednesday.
Aurora runs 15 hospitals and 172 clinics and has more than 1,500 employed physicians within its network, as well as affiliations with 3,000 independent physicians.
Arise Health…
Source: bizjournals.com Health Care:Biotechnology headlines - January 23, 2013 Category: Biotechnology Authors: Rich Kirchen Source Type: research
Alverno launches newly designed RN to BSN program
Alverno College is launching a redesigned RN to BSN program this June. The 18-month program, which consists of a blend of online and in-classroom learning, is structured so that working registered nurses can earn bachelor's degrees.
"This program was prepared based on national nursing standards and a commitment to excellence, in combination with the voice of practicing nurses who told us what they needed in a program to fit their busy lives," Patricia Schroeder, dean of Alverno College's JoAnn McGrath…
Source: bizjournals.com Health Care:Biotechnology headlines - January 23, 2013 Category: Biotechnology Authors: Wendy Strong Source Type: research
MBF Healthcare to buy Universal HMO, more than double membership
MBF Healthcare Partners could more than double the HMO membership that it oversees as it signed a letter of intent to acquire certain assets of Universal Health Care Group.
The Coral Gables-based private equity firm, led by Chairman Mike B. Fernandez, already owns the Simply Healthcare Plans HMO, which offers Medicaid plans, and Better Health, a provider service organization plan.
Financial terms of the deal for St. Petersburg-based Universal Health Care weren’t disclosed. The HMO earned $3 million…
Source: bizjournals.com Health Care:Biotechnology headlines - January 23, 2013 Category: Biotechnology Authors: Brian Bandell Source Type: research
Former Practice Fusion CMO Robert Rowley lands at LifeNexus Inc.
Robert Rowley, M.D., who had a high-profile role at San Francisco’s Practice Fusion until recently, has surfaced at consumer health card company LifeNexus Inc. as medical advisor.
LifeNexus, an eight-year-old company focusing on “portable health records,”also this week announced the hiring of tech veteran John Sines as CFO. He actually started in the job several weeks ago.
David Strand, the company’s chief executive officer, is a former CEO of ExperiaHealth and LifeMasters Supported SelfCare,…
Source: bizjournals.com Health Care:Biotechnology headlines - January 23, 2013 Category: Biotechnology Authors: Chris Rauber Source Type: research
Highmark revises plan to buy WPAHS
Highmark’s offer to put up $75 million for medical education at West Penn Allegheny Health System would be converted into a grant under a revised deal to buy the hospital network, which was disclosed Wednesday in filings with the state.
Highmark also will pay the ailing hospital system $50 million in rate increases over five years while deferring principal and interest payments until July 1 on WPAHS bonds held by the health insurer.
How long the Insurance Department’s review will take is uncertain,…
Source: bizjournals.com Health Care:Biotechnology headlines - January 23, 2013 Category: Biotechnology Authors: Kris B. Mamula Source Type: research
Allergen to buy Map Pharmaceuticals for $958M
Allergan Inc. has agreed to buy Mountain View's Map Pharmaceuticals for $958 million, propelled by the expected Food and Drug Administration approval of Map's inhaled treatment for migraines.
Allergan will snap up Map shares for $25 per share, a 60 percent premium. A treatment for migraines that was a joint project for the two companies, Levadex, is up for approval by the FDA no later than April 15.
Check out Ron Leuty's story in our sister publication, the San Francisco Business Times, for the…
Source: bizjournals.com Health Care:Biotechnology headlines - January 23, 2013 Category: Biotechnology Authors: Jon Xavier Source Type: research
Electrospray ionization mass spectrometric exploration of the high-affinity binding of three natural alkaloids with the mRNA G-quadruplex in the BCL2 5'-untranslated region.
CONCLUSIONS: Three natural small molecules were found to have very high binding affinities to the mRNA G-quadruplex and stabilize this structure. The properties of these alkaloids revealed promising potentials to regulate the expression of the BCL2 protein from the posttranscriptional pathway. Copyright © 2013 John Wiley & Sons, Ltd.
PMID: 23322663 [PubMed - in process]
Source: Rapid Communications in Mass Spectrometry : RCM - January 23, 2013 Category: Chemistry Authors: Tan W, Yuan G Tags: Rapid Commun Mass Spectrom Source Type: research
Multiplex electrochemiluminescence immunoassay of two tumor markers using multicolor quantum dots as labels and graphene asconductingbridge.
Abstract
A multiplex electrochemiluminescence (ECL) immunoassay for simultaneous determination of two different tumor markers, alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA), using multicolor quantum dots as labels and graphene as conducting bridge was developed. Herein, a typical sandwich immune complex was constructed on the glass carbon electrode, with QDs(525) and QDs(625) labeled on secondary anti-AFP and anti-CEA antibodies, respectively, thus to obtain distinguishable ECL signals. Because most of those QDs labeled on secondary antibodies were beyond the space domain of the ECL reaction, graphene ...
Source: Biosensors and Bioelectronics - January 23, 2013 Category: Biotechnology Authors: Guo Z, Hao T, Du S, Chen B, Wang Z, Li X, Wang S Tags: Biosens Bioelectron Source Type: research
Origin and diversification of a metabolic cycle in oligomer world.
Abstract
Based on the oligomer-world hypothesis we propose an abstract model where the molecular recognition among oligomers is described in the shape space. The origin of life in the oligomer world is regarded as the establishment of a metabolic cycle in a primitive cell. The cycle is sustained by the molecular recognition. If an original cell acquires the ability of the replication of oligomers, the relationship among oligomers changes due to the poor fidelity of the replication. This change leads to the diversification of metabolic cycles. The selection among diverse cycles is the basis of the evolution. The evo...
Source: Biosystems - January 23, 2013 Category: Biotechnology Authors: Nishio T, Narikiyo O Tags: Biosystems Source Type: research
A novel analytical method for evolutionary graph theory problems.
Abstract
Evolutionary graph theory studies the evolutionary dynamics of populations structured on graphs. A central problem is determining the probability that a small number of mutants overtake a population. Currently, Monte Carlo simulations are used for estimating such fixation probabilities on general directed graphs, since no good analytical methods exist. In this paper, we introduce a novel deterministic framework for computing fixation probabilities for strongly connected, directed, weighted evolutionary graphs under neutral drift. We show how this framework can also be used to calculate the expected number ...
Source: Biosystems - January 23, 2013 Category: Biotechnology Authors: Shakarian P, Roos P, Moores G Tags: Biosystems Source Type: research
In vitro and in vivo inoculation of four endophytic bacteria on Lycopersicon esculentum.
Abstract
Four bacteria selected on the basis of their capability of fixing atmospheric nitrogen, stimulating plant-growth, and protecting the host plant from pathogens -Azospirillum brasilense, Gluconacetobacter diazotrophicus, Herbaspirillum seropedicae, Burkholderia ambifaria - were inoculated on tomato seeds either singularly, in couple and in a four bacteria mixer. Aim of this research was to evaluate: (1) effect of single and mixed cultures on the inoculated plant - plant growth, dry weight, root length and surface, number of leaves, among others; (2) colonization and interactions of the bacteria inside the ho...
Source: New Biotechnology - January 23, 2013 Category: Biotechnology Authors: Botta AL, Santacecilia A, Ercole C, Cacchio P, Del Gallo M Tags: N Biotechnol Source Type: research
Preparation, Characterization, and Bacteriostasis of AgNP-Coated β-CD Grafting Cellulose Beads.
Abstract
A novel functional material of β-cyclodextrin (β-CD) grafting cellulose beads containing immobilized silver nanoparticles (AgNPs) is presented in this paper. The morphology was characterized by scanning electron microscopy, energy-dispersive X-ray, and X-ray photoelectron spectroscopy. Phenolphthalein probe molecule technique was used to detect the activity of the grafting β-CD, and the results demonstrated that the deposition of AgNPs had no influence on its encapsulation ability. Acid resistance of the AgNPs on the bead material was studied by atomic absorption spectrometry. The stability of the AgNPs...
Source: Applied Biochemistry and Biotechnology - January 23, 2013 Category: Biochemistry Authors: Wang T, Li B, Lin L Tags: Appl Biochem Biotechnol Source Type: research
Structural Insights on Mycobacterium tuberculosis Thiazole Synthase-A Molecular Dynamics/Docking Approach.
Abstract
Tuberculosis (TB), an epidemic disease, affects the world with death rate of two million people every year. The bacterium Mycobacterium tuberculosis was found to be a more potent and disease-prolonged bacterium among the world due to multi-drug resistance. Emergence of new drug targets is needed to overcome the bacterial resistance that leads to control epidemic tuberculosis. The pathway thiamine biosynthesis was targeting M. tuberculosis due to its role in intracellular growth of the bacterium. The screening of enzymes involved in thiamin biosynthesis showed novel target thiazole synthase (ThiG) involved ...
Source: Applied Biochemistry and Biotechnology - January 23, 2013 Category: Biochemistry Authors: Rohini K, Srikumar PS Tags: Appl Biochem Biotechnol Source Type: research
Characterization of Novel EGs Reconstructed from Bacillus subtilis Endoglucanase.
Abstract
Bacterial cellulases have taken on satisfactory application performance and economic value in detergent industry. Neutral endoglucanase (EG1) gene was cloned from Bacillus subtilis and expressed Pichia pastoris in our previous study. Redesigned endoglucanases enhanced cellulase domain, added and deleted carbohydrate-binding module (CBM), named EG2, EG3, and EG4, respectively, were constructed in this study. The redesigned EG genes were expressed in P. pastoris, and their characters were also discussed. The optimal temperature and pH value of the all EGs was 65 °C and 6.0, respectively, where their enzyma...
Source: Applied Biochemistry and Biotechnology - January 23, 2013 Category: Biochemistry Authors: Zi-Zhong T, Zhen-Fang W, Hui C, Xin L, Xue-Yi H, Qi W Tags: Appl Biochem Biotechnol Source Type: research
Plant Regeneration Through Callus Organogenesis and True-to-Type Conformity of Plants by RAPD Analysis in Desmodium gangeticum (Linn.) DC.
Abstract
An efficient plant regeneration protocol was established for an endangered ethnomedicinal plant Desmodium gangeticum (Linn.) DC. Morphogenic calli were produced from 96 % of the cultures comprising the immature leaf explants on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (4.0 mg l(-1)) in combination with 6-benzylaminopurine (BA; 0.8 mg l(-1)). For callus regeneration, various concentrations of BA (1.0-5.0 mg l(-1)) or thidiazuron (TDZ; 1.0-5.0 mg l(-1)) alone or in combination with indole-3-acetic acid (IAA; 0.2-1.0 mg l(-1)) were used. Highest response...
Source: Applied Biochemistry and Biotechnology - January 23, 2013 Category: Biochemistry Authors: Cheruvathur MK, Abraham J, Thomas TD Tags: Appl Biochem Biotechnol Source Type: research
RNA Interference-A Silent but an Efficient Therapeutic Tool.
Abstract
RNA interference (RNAi) is an evolutionary conserved gene regulation pathway that has emerged as an important discovery in the field of molecular biology. One of the important advantages of RNAi in therapy is that it brings about efficient downregulation of gene expression by targeting complementary transcripts in comparison with other antisense-based techniques. RNAi can be can be achieved by introducing chemically synthesized small interfering RNAs (siRNAs) into a cell system. A more stable knockdown effect can be brought about by the use of plasmid or viral vectors encoding the siRNA. RNAi has been used...
Source: Applied Biochemistry and Biotechnology - January 23, 2013 Category: Biochemistry Authors: Ramachandran PV, Ignacimuthu S Tags: Appl Biochem Biotechnol Source Type: research
Preservation of immunorecognition by transferring cells from 10% neutral buffered formalin to 70% ethanol.
Abstract
Prolonged fixation of cells and tissues in 10% neutral buffered formalin (NBF) may decrease immunorecognition in some antigen-antibody pairs. Short fixation in 10% NBF followed by transfer to 70% ethanol has been used to overcome these effects, but the effects of this transfer on immunorecognition have not been explored adequately. We used two cell lines, DU145 (prostate cancer) and SKOV3 (ovarian cancer), grew them on coverslips and fixed them with 10% NBF at room temperature for 5 min and 12, 15, 18, 36, 108 and 180 h. Aliquots of the same cells were fixed in 10% NBF for 12 h, then transferred to 70% eth...
Source: Biotechnic and Histochemistry - January 23, 2013 Category: Research Authors: Otali D, He Q, Stockard C, Grizzle W Tags: Biotech Histochem Source Type: research
Complexation of C(60) Fullerene with Aromatic Drugs.
Complexation of C(60) Fullerene with Aromatic Drugs.
Chemphyschem. 2013 Jan 23;
Authors: Evstigneev MP, Buchelnikov AS, Voronin DP, Rubin YV, Belous LF, Prylutskyy YI, Ritter U
Abstract
The contributions of various physical factors to the energetics of complexation of aromatic drug molecules with C(60) fullerene are investigated in terms of the calculated magnitudes of equilibrium complexation constants and the components of the net Gibbs free energy. Models of complexation are developed taking into account the polydisperse nature of fullerene solutions in terms of the continuous or discrete (fractal) aggre...
Source: Chemphyschem - January 23, 2013 Category: Chemistry Authors: Evstigneev MP, Buchelnikov AS, Voronin DP, Rubin YV, Belous LF, Prylutskyy YI, Ritter U Tags: Chemphyschem Source Type: research
Erratum to: Identification of a newly isolated erythritol-producing yeast and cloning of its erythrose reductase genes.
PMID: 23341192 [PubMed - as supplied by publisher]
Source: Journal of Industrial Microbiology and Biotechnology - January 23, 2013 Category: Microbiology Authors: Deng H, Han Y, Liu Y, Jia W, Zhou Z Tags: J Ind Microbiol Biotechnol Source Type: research
Metagenomic exploration reveals high levels of microbial arsenic metabolism genes in activated sludge and coastal sediments.
This study provided a novel perspective on understanding the ecology of arsenic metabolism in different water environments using high-throughput sequencing technique.
PMID: 23340578 [PubMed - as supplied by publisher]
Source: Applied Microbiology and Biotechnology - January 23, 2013 Category: Microbiology Authors: Cai L, Yu K, Yang Y, Chen BW, Li XD, Zhang T Tags: Appl Microbiol Biotechnol Source Type: research
Biotechnology: Cancer drugs from algae
Nature 493, 7433 (2013). doi:10.1038/493455e
Highly read on www.pnas.org in DecemberAlgae could be used to make complex, targeted cancer drugs, thanks to their photosynthetic organelles, the chloroplasts.Immunotoxins are drugs that combine a toxin with an antibody; the antibody targets the toxin to specific cells, such as cancer
Source: Nature - January 23, 2013 Category: Research Tags: Research Highlights Source Type: research
Do you believe in magic? GOP vows to balance budget without raising taxes
House Republicans today committed to balancing the federal budget within 10 years without raising taxes.
And I'm going to beat out Robert Griffin III next year for the Washington Redskins' starting quarterback job.
Why not? If Republicans can balance the budget without raising taxes, then I can surely outplay the NFL's Rookie of the Year. Why not? Both goals are equally unrealistic.
To put things in perspective: The budget plan crafted last year by Paul Ryan, House Budget Committee chairman, doesn't…
Source: bizjournals.com Health Care:Biotechnology headlines - January 22, 2013 Category: Biotechnology Authors: Kent Hoover Source Type: research
BioMarin seeks to test enzyme-replacement therapy in Batten Disease patients
BioMarin Pharmaceutical Inc. doesn't back down from a tough rare disease.
The Novato company (NASDAQ: BMRN), which made its name with enzyme-replacement therapies that tackle virtually unpronounceable orphan diseases like phenylketonuria and versions of mucopolysaccharidosis, expects to enroll the first patient by mid-year in an early-stage clinical trial in Batten Disease.
CEO Jean-Jacques Bienaimé said BioMarin will file an investigational new drug application with the Food and Drug Administration…
Source: bizjournals.com Health Care:Biotechnology headlines - January 22, 2013 Category: Biotechnology Authors: Ron Leuty Source Type: research
Allergan to buy migraine drug developer MAP Pharmaceuticals for $958 million
Allergan Inc. will buy MAP Pharmaceuticals Inc. for about $958 million on the cusp of the Mountain View company winning regulator approval of its inhaled migraine drug.
Irvine-based Allergan (NYSE: AGN) said Tuesday the deal will pay shareholders of MAP (NASDAQ: MAPP) $25 per share. The price is a 60 percent premium over MAP's Tuesday closing price of $15.58 per share.
The acquisition is expected to close late in the first quarter or early in the second quarter, Allergan said in a press release.
Allergan,…
Source: bizjournals.com Health Care:Biotechnology headlines - January 22, 2013 Category: Biotechnology Authors: Ron Leuty Source Type: research
Silverado Senior Living breaks ground in Peoria, will employ 100
Silverado Senior Living broke ground today on a new, $12 million assisted living facility in Peoria, which upon completion will employ roughly 100.
The Irvine, Calif.-based company specializes in assisted living communities for those who suffer from memory-impairing diseases such as Alzheimer’s disease. Silverado’s latest community will be at 13391 North 94th Drive near the cluster of medical offices and facilities in Peoria’s west end.
The 43,500-square-foot Peoria facility will be the company’s…
Source: bizjournals.com Health Care:Biotechnology headlines - January 22, 2013 Category: Biotechnology Authors: Tim Gallen Source Type: research
Moody's: Outlook for nonprofit hospitals remains negative
Moody’s Investors Service has issued its 2013 outlook for nonprofit hospitals and reports the outlook will remain negative.
Moody’s attributes the negative outlook to diminishing growth despite positive revenues, limited reimbursement from insurers and federal cuts to medical spending. Also aiding the negative outlook is high unemployment that softens demand.
“Our sector outlook has been negative since 2008, reflecting the lasting impact of the recession on patient volumes, significant challenges…
Source: bizjournals.com Health Care:Biotechnology headlines - January 22, 2013 Category: Biotechnology Authors: Samantha Liss Source Type: research
Mercy's $27 million birthing suite renovation set to open
Mercy Hospital St. Louis is opening its new Maternity Welcome Center and Labor and Birth Suites Jan. 28.
The $27 million project for the 75,000 square-foot renovation spans three floors that house 31 new labor and birth suites, six surgical suites and 11 private pre-op and recovery rooms. When soon-to-be moms enter the main lobby, they have easy access to four interview and 12 exam rooms where moms can check-in and be evaluated before moving to the labor and birth suites.
After giving birth, moms…
Source: bizjournals.com Health Care:Biotechnology headlines - January 22, 2013 Category: Biotechnology Authors: Samantha Liss Source Type: research
PacificSource adds 40 jobs
Eugene-based PacificSource Health Plans announced it is adding 40 full-time employees following a successful Medicare enrollment period.
The new employees will be charged with managing incoming business from the 16,000 new Medicare customers recently added to PacificSource systems in Oregon and Idaho.
PacificSource entered the Medicare market with its December 2009 acquisition of Bend-based Clear One Health Plans and has been building that portion of the business steadily. PacificSource spokeswoman…
Source: bizjournals.com Health Care:Biotechnology headlines - January 22, 2013 Category: Biotechnology Authors: Andy Giegerich Source Type: research
Doctors, techies spend day lobbying at Texas Capitol
Health information technologists from across Texas descended upon the Capitol in Austin Tuesday to try to shape the future of patient care in the Lone Star state.
Physicians, nurses and IT experts used Texas Health Information Technology Day to lobby state legislators about health IT’s ability to decrease costs while increasing quality of care.
A 2012 report by Texas State University in San Marcos, near Austin, estimated that 10,000 additional health IT workers will be needed in the $103.6 billion…
Source: bizjournals.com Health Care:Biotechnology headlines - January 22, 2013 Category: Biotechnology Authors: James Jeffrey Source Type: research
Texas Children's sets date for ribbon cutting in Pearland
Source: bizjournals.com Health Care:Biotechnology headlines - January 22, 2013 Category: Biotechnology Authors: Bayan Raji Source Type: research
Statewide health record system shares information across state lines
Missouri Health Connection (MHC) is now able to exchange data with neighboring state health information exchanges.
The MHC, Nebraska Health Information Initiative (NeHII) and Kansas Health Information Network (KHIN) are now able to send direct secured messages across state lines, according to officials.
Statewide health information exchanges are designed to be the one-stop shop for patient medical records. The goal is to improve patient care by allowing providers, regardless of geographic location,…
Source: bizjournals.com Health Care:Biotechnology headlines - January 22, 2013 Category: Biotechnology Authors: Samantha Liss Source Type: research
From laboratory to pilot plant E. coli fed-batch cultures: optimizing the cellular environment for protein maximization.
Abstract
For recombinant protein production in E. coli fed-batch cultures, post-induction conditions have great influence in the quantity and quality of the product. The present paper covers the effect of different factors affecting the cellular environment in recombinant aldolase (rhamnulose-1-phosphate aldolase, RhuA) production. An operational mode employing an exponential addition profile for constant specific growth rate has been analyzed, in order to understand and define possible modifications with influence on post-induction cellular behavior. A constant addition profile has been demonstrated to render high...
Source: Journal of Industrial Microbiology and Biotechnology - January 22, 2013 Category: Microbiology Authors: Ruiz J, Fernández-Castané A, de Mas C, González G, López-Santín J Tags: J Ind Microbiol Biotechnol Source Type: research
