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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 8.

Controllable self-assembly of amphiphilic macrocycles into closed-shell and open-shell vesicles, nanotubes, and fibers
, 2013, Accepted ManuscriptDOI: 10.1039/C3CC40535D, CommunicationAtanu Mitra, Dillip K Panda, Lucas J. Corson, Sourav SahaDepending on functional groups, amphiphilic hexaamide macrocycles self-assemble into closed-shell and open-shell vesicles in polar solvents. In the presence of water, open-shell vesicles morph into closed-shell vesicles, whereas acidification of...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2013 Category: Chemistry Authors: Atanu Mitra Source Type: research

A fluorescent probe for rapid aqueous fluoride detection and cell imaging
Chem. Commun., 2013, 49,2494-2496DOI: 10.1039/C2CC37270C, CommunicationBowen Ke, Weixuan Chen, Nanting Ni, Yunfeng Cheng, Chaofeng Dai, Hieu Dinh, Binghe WangA stable and highly selective fluorescent probe has been designed and synthesized for the rapid detection of fluoride ions (F-) in aqueous solution and living cells.The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2013 Category: Chemistry Authors: Bowen Ke Source Type: research

Ion-exchanged route synthesis of Fe2N/N-doped graphitic nanocarbons composite as advanced oxygen reduction electrocatalyst
, 2013, Accepted ManuscriptDOI: 10.1039/C3CC40971F, CommunicationLei Wang, Jie Yin, Lu Zhao, Chungui Tian, Peng Yu, Jian-Qiang Wang, Honggang FuFe2N nanoparticles and nitrogen-doped graphitic nanosheet composites (Fe2N/NGC) have been synthesized by an ion-exchanged route, which can serve as efficient non-precious metal electrocatalyst with 4e- reaction pathway for oxygen reduction...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2013 Category: Chemistry Authors: Lei Wang Source Type: research

Lanthanide Corroles: A New Class of Macrocyclic Lanthanide Complexes
, 2013, Accepted ManuscriptDOI: 10.1039/C3CC38806A, CommunicationJohn Arnold, Heather L Buckley, Mitchell R Anstey, Daniel T GrykoThe first examples of lanthanide corroles are prepared by two synthetic routes. (Mes2(p-OMePh)corrole)La[middle dot]3DME (1[middle dot]3DME) and (Mes2(p-OMePh)corrole)Tb[middle dot]4DME (2[middle dot]4DME) are prepared from the free base corrole and Ln((NSiMe3)2)3, while (Mes2(p-OMePh)corrole)Gd[middle dot]TACNMe3 (3[middle dot]TACNMe3) is...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2013 Category: Chemistry Authors: John Arnold Source Type: research

In vivo imaging using polymeric nanoparticles stained with near-infrared chemiluminescent and fluorescent squaraine catenane endoperoxide
, 2013, Accepted ManuscriptDOI: 10.1039/C3CC40630J, CommunicationJung-Jae Lee, Alexander White, Douglas Rice, BD SmithPolystyrene nanoparticles stained with squaraine catenane endoperoxide exhibit remarkably high chemiluminescence and enable optical imaging of biodistribution in living mice. Whole-body chemiluminescence imaging was much more effective than fluorescence at...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2013 Category: Chemistry Authors: Jung-Jae Lee Source Type: research

Palladium-Catalyzed Insertion of N-Tosylhydrazones for the Synthesis of Isoindolines
, 2013, Accepted ManuscriptDOI: 10.1039/C3CC40577J, CommunicationYong-Ming Liang, Ping-Xin Zhou, Jian-Yi Luo, Lian-Biao Zhao, Yu-Ying YeIsoindolines are synthesized by palladium-catalyzed coupling reaction of N-(2-iodobenzyl) anilines with [small alpha], [small beta]-unsaturated N-tosylhydrazones. The reaction has several potential advantages: 1) Toleration of a wide range of functional groups. 2)...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2013 Category: Chemistry Authors: Yong-Ming Liang Source Type: research

Antimicrobial N-(2-chlorobenzyl)-substituted hydroxamate is an inhibitor of 1-deoxy-D-xylulose 5-phosphate synthase
, 2013, Accepted ManuscriptDOI: 10.1039/C3CC40758F, CommunicationJunko Ohkanda, Daisuke Hayashi, Nobuo Kato, Tomohisa Kuzuyama, Yasuo SatoN-(2-Chlorobenzyl)-substituted hydroxamate, readily produced by hydrolysis of ketoclomazone, was identified as an inhibitor of 1-deoxy-D-xylulose 5-phosphate synthase (DXS), with an IC50 value of 1.0 M. The compound inhibited the growth...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2013 Category: Chemistry Authors: Junko Ohkanda Source Type: research

Au nanoparticles embedded into the inner wall of TiO2 hollow spheres as nanoreactor with superb thermal stability
, 2013, Accepted ManuscriptDOI: 10.1039/C3CC39212K, CommunicationYu Yu, Changyan Cao, Zhe Chen, Hua Liu, Ping Li, Zhifeng Dou, Weiguo SongA new nanoreactor type composite catalyst with Au NPs being embedded into the inner wall of the mesoporous TiO2 hollow spheres resulted in enhanced synergistic effect and superb thermal stability...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2013 Category: Chemistry Authors: Yu Yu Source Type: research

Visualizing Nanoparticle Mobility in Liquid at Atomic Resolution
, 2013, Accepted ManuscriptDOI: 10.1039/C3CC41136B, CommunicationMadeline J. Dukes, Benjamin W Jacobs, David G Morgan, Harshad Hegde, Deborah F. KellyGold nanorods are widely known for their photothermal properties to treat solid tumors. Our work demonstrates the unrealized capacity to image these reagents in liquid at high resolution using Transmission...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2013 Category: Chemistry Authors: Madeline J. Dukes Source Type: research

Chemically reactive spinning dope for significant improvements in wet spun carbon nanotube fibres
, 2013, Accepted ManuscriptDOI: 10.1039/C3CC38953G, CommunicationJose Miguel Gonzalez-Dominguez, Wilfrid Neri, Maryse Maugey, Philippe Poulin, Alejandro Anson, Maria Teresa MartinezSingle-walled carbon nanotubes can be spun in a polyvinyl alcohol stream to produce nanocomposite fibres. In this frame, we use a facile ester linking between both elements to create improved...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2013 Category: Chemistry Authors: Jose Miguel Gonzalez-Dominguez Source Type: research

Ruthenium-catalyzed intramolecular selective halogenation of O-methylbenzohydroximoyl halides: a new route to halogenated aromatic nitriles
, 2013, Accepted ManuscriptDOI: 10.1039/C3CC41124A, CommunicationMasilamani Jeganmohan, Ravi Kiran Chinnagolla, Sandeep PimparkarThe intramolecular halogenation of O-methylbenzohydroximoyl halides in the presence of catalytic amount of [{RuCl2(p-cymene)}2] and diphenylacetylene or methyl acrylate gave halo substituted aromatic nitriles in good to excellent yields in...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Chem. Commun. latest articles - March 1, 2013 Category: Chemistry Authors: Masilamani Jeganmohan Source Type: research

Hairpin oligonucleotides anchored terbium ion: a fluorescent probe to specifically detect lead(II) at sub-nM levels
Analyst, 2013, Advance ArticleDOI: 10.1039/C3AN36795A, PaperYueteng Wei, Ru Liu, Yaling Wang, Yuliang Zhao, Zhifang Cai, Xueyun GaoTb-GH5 (GH5: designed oligonucleotides with hairpin structure) probe emits bright, sharp fluorescence. Lead ions compete with terbium ions causing the terbium ions to depart from GH5 decreasing fluorescence.To cite this article before page numbers are assigned, use the DOI form of citation above.The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Analyst latest articles - March 1, 2013 Category: Chemistry Authors: Yueteng Wei Source Type: research

Hybridization chain reaction-based instantaneous derivatization technology for chemiluminescence detection of specific DNA sequences
, 2013, Accepted ManuscriptDOI: 10.1039/C3AN36885H, PaperXin Wang, Choiwan Lau, Masaaki Kai, Jianzhong LuWe propose here a new amplifying strategy that uses hybridization chain reaction (HCR) to detect specific sequences of DNA, where stable DNA monomers assemble on the magnetic beads only upon...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Analyst latest articles - March 1, 2013 Category: Chemistry Authors: Xin Wang Source Type: research

Magnetic nanoparticle-enhanced SPR on gold nanoslit for ultra-sensitive, label-free detection of nucleic acid biomarkers
, 2013, Accepted ManuscriptDOI: 10.1039/C3AN36655C, PaperMansoureh Z Mousavi, Huai-Yi Chen, Shu-Han Wu, Kuang-Li Lee, Pei-Kuen Wei, Ji-Yen ChengWe have demonstrated a detection method for the ultra-sensitive detection of an mRNA biomarker. The method utilizes functionalized magnetic nanoparticles (MNPs) for signal enhancement in conjunction with surface plasmon resonance...The content of this RSS Feed (c) The Royal Society of Chemistry
Source: RSC - Analyst latest articles - March 1, 2013 Category: Chemistry Authors: Mansoureh Z Mousavi Source Type: research

Finding absestos in soil
A new soil analysis technique could allow the detection of very low levels of asbestos, which could be harmful to health
Source: Chemistry World | Latest News - March 1, 2013 Category: Chemistry Authors: Rebecca Brodie Source Type: research

Chemical transport defines ‘Goldilocks’ cell size
Too big and macromolecules like proteins and DNA have to travel too far, too small and they’re too crowded
Source: Chemistry World | Latest News - March 1, 2013 Category: Chemistry Authors: Andy Extance Source Type: research

Does chemical regulation boost innovation?
Report suggests that supply of innovation responds to extra demand created by new laws
Source: Chemistry World | Latest News - March 1, 2013 Category: Chemistry Authors: Andrew Turley Source Type: research

Lyconadin A
Paul Docherty tackles a serious repeat offender
Source: Chemistry World | Latest News - March 1, 2013 Category: Chemistry Authors: Paul Docherty Source Type: research

25 years ago: Roald Hoffmann publishes his poetry
The Nobel prize winner shows his artistic side
Source: Chemistry World | Latest News - March 1, 2013 Category: Chemistry Source Type: research

The magic of microwaves
Do the devices just heat cups of tea or is there more to their use in chemistry?
Source: Chemistry World | Latest News - March 1, 2013 Category: Chemistry Source Type: research

Molecular basis for prey relocation in viperid snakes
Viperid snakes bite their prey and then let them go to avoid retaliation. By measuring snake behavior and analysing venom chemistry, Stephen Mackessy and colleagues provide an answer to how they find them again.
Source: BMC Biology - Latest articles - March 1, 2013 Category: Biology Authors: Anthony SaviolaDavid ChiszarChardelle BuschStephen Mackessy Source Type: research

Poly[[diaquabis(μ2-4,4′-bipyridyl)cobalt(II)] dinitrate tetrahydrate]
The title compound, {[Co(C10H8N2)2(H2O)2](NO3)2·4H2O}n, (C10H8N2 = 4,4′-bipyridine = 4,4′-bpy) is a layered coordination polymer built up from a cationic square grid extending in (101) enclosing uncoordinating nitrate ions and water molecules. The CoII ion has site symmetry 2 and one of the 4,4′-bpy ligands is generated by twofold symmetry [two N atoms and two C atoms lie on the rotation axis and the dihedral angle between the pyridine rings is 45.66 (5)°]. The other 4,4′-bpy ligand is generated by a crystallographic inversion center. The CoII ion exhibits a slightly distorted octahedral coordination geometry d...
Source: Acta Crystallographica Section E - March 1, 2013 Category: Chemistry Authors: Lehleh, A.Boutebdja, M.Beghidja, A.Beghidja, C.Merazig, H. Tags: metal-organic compounds Source Type: research

Metabolic fingerprinting of Angelica sinensis during growth using UPLC-TOFMS and chemometrics data analysis
Conclusions: The UPLC-QTOFMS based metabolomics approach has great potential for analysis of the alterations of secondary metabolites of Angelica sinensis during growth
Source: Chemistry Central Journal - March 1, 2013 Category: Chemistry Authors: Yiyun QianYali WangRina SaHui YanXinbo PanYingwen YangYujing Sun Source Type: research

Validation of an analytical method based on the high-resolution continuum source flame atomic absorption spectrometry for the fast-sequential determination of several hazardous/priority hazardous metals in soil
Conclusions: High-resolution continuum source flame atomic absorption spectrometry can be successfully used for the rapid, multielemental determination of hazardous/priority hazardous metals in soil with similar analytical performances to those in inductively coupled plasma optical emission spectrometry.
Source: Chemistry Central Journal - March 1, 2013 Category: Chemistry Authors: Tiberiu FrentiuMichaela PontaRaluca Hategan Source Type: research

The multiple roles of histidine in protein interactions
Among the 20 natural amino acids histidine is the most active and versatile member that plays the multiple roles in protein interactions, often the key residue in enzyme catalytic reactions. A theoretical and comprehensive study on the structural features and interaction properties of histidine is certainly helpful. Four interaction types of histidine are quantitatively calculated, including: (1) Cation-pi interactions, in which the histidine acts as the aromatic pi-motif in neutral form (His), or plays the cation role in protonated form (His+); (2) pi-pi stacking interactions between histidine and other aromatic amino aci...
Source: Chemistry Central Journal - March 1, 2013 Category: Chemistry Authors: Si-Ming LiaoQi-Shi DuJian-Zong MengZong-Wen PangRi-Bo Huang Source Type: research

Friedel–Crafts Chemistry. Part 39. Unprecedented Facile Route to the Synthesis of Benzo[b][1]benzazepines via Intramolecular Friedel–Crafts Cyclialkylations
Hassan A. K. Abd El-Aal, Ali A. Khalaf An efficient methodology for the synthesis of some novel substituted benzo[b][1]benzazepin-5-ones and benzo[b][1]benzazepines via intramolecular Friedel–Crafts cyclialkylations and cycliacylations of suitable synthesized carboxylic acids and tertiary alcohols catalyzed by Brönsted and Lewis acid catalysts is presented.
Source: Australian Journal of Chemistry - March 1, 2013 Category: Chemistry Authors: Hassan A. K. Abd El-AalAli A. Khalaf Source Type: research

Optimization of IL-12 Expression [Immunology]
IL-12 is a 70-kDa heterodimeric cytokine composed of the p35 and p40 subunits. To maximize cytokine production from plasmid DNA, molecular steps controlling IL-12p70 biosynthesis at the posttranscriptional and posttranslational levels were investigated. We show that the combination of RNA/codon-optimized gene sequences and fine-tuning of the relative expression levels of the two subunits within a cell resulted in increased production of the IL-12p70 heterodimer. We found that the p40 subunit plays a critical role in enhancing the stability, intracellular trafficking, and export of the p35 subunit. This posttranslational re...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Jalah, R., Rosati, M., Ganneru, B., Pilkington, G. R., Valentin, A., Kulkarni, V., Bergamaschi, C., Chowdhury, B., Zhang, G.-M., Beach, R. K., Alicea, C., Broderick, K. E., Sardesai, N. Y., Pavlakis, G. N., Felber, B. K. Tags: Gene Regulation Source Type: research

Ligand Entry in Myoglobin [Computational Biology]
Since the elucidation of the myoglobin (Mb) structure, a histidine residue on the E helix (His-E7) has been proposed to act as a gate with an open or closed conformation controlling access to the active site. Although it is believed that at low pH, the His-E7 gate is in its open conformation, the full relationship between the His-E7 protonation state, its conformation, and ligand migration in Mb is hotly debated. We used molecular dynamics simulations to first address the effect of His-E7 protonation on its conformation. We observed the expected shift from the closed to the open conformation upon protonation, but more impo...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Boechi, L., Arrar, M., Marti, M. A., Olson, J. S., Roitberg, A. E., Estrin, D. A. Tags: Molecular Biophysics Source Type: research

p38{alpha} MAPK Promotes Angiogenesis [Signal Transduction]
LY2228820 dimesylate is a highly selective small molecule inhibitor of p38α and p38β mitogen-activated protein kinases (MAPKs) that is currently under clinical investigation for human malignancies. p38 MAPK is implicated in a wide range of biological processes, in particular those that support tumorigenesis. One such process, angiogenesis, is required for tumor growth and metastasis, and many new cancer therapies are therefore directed against the tumor vasculature. Using an in vitro co-culture endothelial cord formation assay, a surrogate of angiogenesis, we investigated the role of p38 MAPK in growth factor- and tumor-...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Tate, C. M., Blosser, W., Wyss, L., Evans, G., Xue, Q., Pan, Y., Stancato, L. Tags: Cell Biology Source Type: research

Functional Interaction of PSA and MARCKS [Membrane Biology]
Polysialic acid (PSA) is a homopolymeric glycan that plays crucial roles in the developing and adult nervous system. So far only a few PSA-binding proteins have been identified. Here, we identify myristoylated alanine-rich C kinase substrate (MARCKS) as novel PSA binding partner. Binding assays showed a direct interaction between PSA and a peptide comprising the effector domain of MARCKS (MARCKS-ED). Co-immunoprecipitation of PSA-carrying neural cell adhesion molecule (PSA-NCAM) with MARCKS and co-immunostaining of MARCKS and PSA at the cell membrane of hippocampal neurons confirm the interaction between PSA and MARCKS. Co...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Theis, T., Mishra, B., von der Ohe, M., Loers, G., Prondzynski, M., Pless, O., Blackshear, P. J., Schachner, M., Kleene, R. Tags: Neurobiology Source Type: research

Higher Order Chromatin Structure of the Gel-forming Mucin Gene [Gene Regulation]
Four of the genes that encode gel-forming mucins, which are major components of the mucus layer protecting many epithelial surfaces, are clustered at chromosome 11p15.5 and show both cell- and tissue-specific expression patterns. We aimed to determine whether the individual genes were coordinately regulated by mechanisms involving higher order chromatin structure. CCCTC-binding factor (CTCF) sites were predicted in silico and CTCF occupancy then evaluated by chromatin immunoprecipitation. CTCF was found at many sites across the gene cluster, and its binding was correlated with mucin gene expression. Next, siRNA-mediated de...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Gosalia, N., Leir, S.-H., Harris, A. Tags: Glycobiology and Extracellular Matrices Source Type: research

Regulation of the G{alpha}s Promoter [Molecular Bases of Disease]
The onset of parturition is associated with a number of proinflammatory mediators that are themselves regulated by the nuclear factor κB (NF-κB) family of transcription factors. In this context, we previously reported that the RelA NF-κB subunit represses transcription and mRNA expression of the proquiescent Gαs gene in human myometrial cells following stimulation with the proinflammatory cytokine TNF. In the present study, we initially defined the functional consequence of this on myometrial contractility. Here we show that, contrary to our initial expectations, TNF did not induce myometrial contractility but did inhi...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Webster, S. J., Waite, S. L., Cookson, V. J., Warren, A., Khan, R., Gandhi, S. V., Europe-Finner, G. N., Chapman, N. R. Tags: Gene Regulation Source Type: research

RNAP Active Center Tuning Controls Transcription [RNA]
Precise transcription by cellular RNA polymerase requires the efficient removal of noncognate nucleotide residues that are occasionally incorporated. Mis-incorporation causes the transcription elongation complex to backtrack, releasing a single strand 3′-RNA segment bearing a noncognate residue, which is hydrolyzed by the active center that carries two Mg2+ ions. However, in most x-ray structures only one Mg2+ is present. This Mg2+ is tightly bound to the active center aspartates, creating an inactive stable state. The first residue of the single strand RNA segment in the backtracked transcription elongation complex stro...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Sosunova, E., Sosunov, V., Epshtein, V., Nikiforov, V., Mustaev, A. Tags: Enzymology Source Type: research

REFLECTIONS: Fifty Years Excitement with Science [Protein Synthesis and Degradation]
Introduction I was born on April 2, 1942 in Strasbourg in the sad time when Alsace was occupied and ruled by Hitler's Nazis. Like many in Alsace, my father, who was in the French Army in 1939, became a malgré-nous during Nazi occupation and was forcibly conscripted into the Wehrmacht in 1943. This was retaliation for refusing to wear the German uniform of railway employees while working at his stationmaster job near Strasbourg. He was quickly sent to the front in Russia and killed near Minsk in January 1944. I grew up fatherless in Strasbourg, but my childhood was happy and rich because I received love and affection from ...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Giege, R. Tags: Reflections Source Type: research

Dex Induces miR1-mediated Atrophy [RNA]
High doses of dexamethasone (Dex) or myostatin (Mstn) induce severe atrophy of skeletal muscle. Here we show a novel microRNA1 (miR1)-mediated mechanism through which Dex promotes skeletal muscle atrophy. Using both C2C12 myotubes and mouse models of Dex-induced atrophy we show that Dex induces miR1 expression through glucocorticoid receptor (GR). We further show that Mstn treatment facilitates GR nuclear translocation and thereby induces miR1 expression. Inhibition of miR1 in C2C12 myotubes attenuated the Dex-induced increase in atrophy-related proteins confirming a role for miR1 in atrophy. Analysis of miR1 targets revea...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Kukreti, H., Amuthavalli, K., Harikumar, A., Sathiyamoorthy, S., Feng, P. Z., Anantharaj, R., Tan, S. L. K., Lokireddy, S., Bonala, S., Sriram, S., McFarlane, C., Kambadur, R., Sharma, M. Tags: Molecular Bases of Disease Source Type: research

BAR Domain Proteins Are Needed to Recruit Dynamin to the Membrane♦ [Papers of the Week]
♦ See referenced article, J. Biol. Chem. 2013, 288, 6651–6661 Dynamin is a protein involved in a number of membrane fission events, including the cleavage of clathrin-coated vesicles for endocytosis. BAR domain proteins are a class of proteins that have been implicated in the recruitment of dynamin to the membrane. In this Paper of the Week, a group led by Michael Meinecke (who is now at the Georg-August University in Germany) and Harvey T. McMahon at the MRC Laboratory of Molecular Biology in the United Kingdom provided direct evidence that dynamin was recruited to the membrane by two BAR domain proteins, endophilin a...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Tags: Membrane Biology Source Type: research

Membrane Recruitment of Dynamin and BAR Domain Proteins [Membrane Biology]
Dynamin mediates various membrane fission events, including the scission of clathrin-coated vesicles. Here, we provide direct evidence for cooperative membrane recruitment of dynamin with the BIN/amphiphysin/Rvs (BAR) proteins, endophilin and amphiphysin. Surprisingly, endophilin and amphiphysin recruitment to membranes was also dependent on binding to dynamin due to auto-inhibition of BAR-membrane interactions. Consistent with reciprocal recruitment in vitro, dynamin recruitment to the plasma membrane in cells was strongly reduced by concomitant depletion of endophilin and amphiphysin, and conversely, depletion of dynamin...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Meinecke, M., Boucrot, E., Camdere, G., Hon, W.-C., Mittal, R., McMahon, H. T. Tags: Membrane Biology Source Type: research

Regulation of RhoGEF Syx Function by 14-3-3 Binding [Signal Transduction]
Syx is a Rho-specific guanine nucleotide exchange factor (GEF) that localizes at cell-cell junctions and promotes junction stability by activating RhoA and the downstream effector Diaphanous homolog 1 (Dia1). Previously, we identified several molecules, including 14-3-3 proteins, as Syx-interacting partners. In the present study, we show that 14-3-3 isoforms interact with Syx at both its N- and C-terminal regions in a phosphorylation-dependent manner. We identify the protein kinase D-mediated phosphorylation of serine 92 on Syx, and additional phosphorylation at serine 938, as critical sites for 14-3-3 association. Our dat...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Ngok, S. P., Geyer, R., Kourtidis, A., Storz, P., Anastasiadis, P. Z. Tags: Cell Biology Source Type: research

Calcium Regulation of MMP-1 Activity [Glycobiology and Extracellular Matrices]
Conditionally active proteins regulated by a physiological parameter represent a potential new class of protein therapeutics. By systematically creating point mutations in the catalytic and linker domains of human MMP-1, we generated a protein library amenable to physiological parameter-based screening. Mutants screened for temperature-sensitive activity had mutations clustered at or near amino acids critical for metal binding. One mutant, GVSK (Gly159 to Val, Ser208 to Lys), contains mutations in regions of the catalytic domain involved in calcium and zinc binding. The in vitro activity of GVSK at 37 °C in high Ca2+ (10 ...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Paladini, R. D., Wei, G., Kundu, A., Zhao, Q., Bookbinder, L. H., Keller, G. A., Shepard, H. M., Frost, G. I. Tags: Enzymology Source Type: research

Ubiquitination by MARCH-8 Controls TRAIL-R1 [Protein Synthesis and Degradation]
We report here that TRAIL-R1 is a physiological substrate of the endogenous MARCH-8 ligase. Human TRAIL-R1 and R2 play a role in immunosurveillance and are targets for cancer therapy, because they selectively induce apoptosis in tumor cells. We demonstrate that TRAIL-R1 is down-regulated from the cell surface, with great preference over TRAIL-R2, by exogenous expression of MARCH ligases that are implicated in endosomal trafficking, such as MARCH-1 and -8. MARCH-8 attenuated TRAIL-R1 cell surface expression and apoptosis signaling by virtue of its ligase activity. This suggested that ubiquitination of TRAIL-R1 was instrumen...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: van de Kooij, B., Verbrugge, I., de Vries, E., Gijsen, M., Montserrat, V., Maas, C., Neefjes, J., Borst, J. Tags: Cell Biology Source Type: research

Reactivation of Na,K-ATPase after Cardiac Glycoside Inhibition [Enzymology]
The Na,K-ATPase is specifically inhibited by cardiotonic steroids (CTSs) like digoxin and is of significant therapeutic value in the treatment of congestive heart failure and arrhythmia. Recently, new interest has arisen in developing Na,K-ATPase inhibitors as anticancer agents. In the present study, we compare the potency and rate of inhibition as well as the reactivation of enzyme activity following inhibition by various cardiac glycosides and their aglycones at different pH values using shark Na,K-ATPase stabilized in the E2MgPi or in the E2BeFx conformations. The effects of the number and nature of various sugar residu...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Cornelius, F., Kanai, R., Toyoshima, C. Tags: Cell Biology Source Type: research

Mapping Polyamine Block of Kir Channels [Signal Transduction]
Steeply voltage-dependent inward rectification of Kir (inwardly rectifying potassium) channels arises from blockade by cytoplasmic polyamines. These polycationic blockers traverse a long (>70 Å) pore, displacing multiple permeant ions, en route to a high affinity binding site that remains loosely defined. We have scanned the effects of cysteine modification at multiple pore-lining positions on the blocking properties of a library of polyamine analogs, demonstrating that the effects of cysteine modification are position- and blocker-dependent. Specifically, introduction of positively charged adducts results in two distinct...
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Kurata, H. T., Akrouh, A., Li, J. B. W., Marton, L. J., Nichols, C. G. Tags: Molecular Biophysics Source Type: research

14-3-3 binding to Ataxin-1 (ATXN1) regulates its dephosphorylation at Ser-776 and transport to the nucleus. [Additions and Corrections]
VOLUME 286 (2011) PAGES 34606–34616 PAGE 34612: It has come to our attention that the labeling of the lines in the graph in Fig. 5C is reversed. The line labeled “Mock ID” should be “PP2A ID” instead. Likewise, the line labeled “PP2A ID” should be marked “Mock ID.”
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Lai, S., O'Callaghan, B., Zoghbi, H. Y., Orr, H. T. Tags: Additions and Corrections Source Type: research

The peroxisome proliferator-activated receptor {gamma} coactivator 1{alpha}/{beta} (PGC-1) coactivators repress the transcriptional activity of NF-{kappa}B in skeletal muscle cells. [Additions and Corrections]
VOLUME 288 (2013) PAGES 2246–2260 PAGE 2256: In the original Fig. 7D, we inadvertently omitted adding a demarcation line to indicate the removal of interjacent bands between the vehicle- and okadaid acid-treated samples of the phospho-p65 Western blot on the same gel. The corrected figure is shown below and does not affect the substance or our interpretation of the results. We apologize for the confusion this may have caused. jbc;288/9/6589/FU1F1FU1
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Eisele, P. S., Salatino, S., Sobek, J., Hottiger, M. O., Handschin, C. Tags: Additions and Corrections Source Type: research

A lectin from the mussel Mytilus galloprovincialis has a highly novel primary structure and induces glycan-mediated cytotoxicity of globotriaosylceramide-expressing lymphoma cells. [Additions and Corrections]
VOLUME 287 (2012) PAGES 44772–44783 As mentioned under the Introduction, Belogortseva et al. (Ref. 10 from our work) reported in 1998 a GalNAc/Gal-binding lectin isolated from Crenomytilus grayanus, a sea mussel related to the genus Mytilus. The primary structure of the lectin was not presented in the 1998 paper. While our paper was in press, we were informed that the mRNA sequence of the Crenomytilus lectin was submitted to GenBankTM by Kovalchuk et al. (Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok, Russia) and appeared on the website on January 30, 2012 (www....
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Fujii, Y., Dohmae, N., Takio, K., Kawsar, S. M. A., Matsumoto, R., Hasan, I., Koide, Y., Kanaly, R. A., Yasumitsu, H., Ogawa, Y., Sugawara, S., Hosono, M., Nitta, K., Hamako, J., Matsui, T., Ozeki, Y. Tags: Additions and Corrections Source Type: research

Characterization of a cytosolic heat-shock protein-caveolin chaperone complex. [Additions and Corrections]
This article has been retracted by the publisher. An investigation by the Office of Research Integrity determined that falsified and/or fabricated Western blots were included in Figs. 1A, 2, A and B, and 4A (https://federalregister.gov/a/2012-28209).
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Uittenbogaard, A., Ying, Y.-s., Smart, E. J. Tags: Additions and Corrections Source Type: research

Cholesteryl ester is transported from caveolae to internal membranes as part of a caveolin-annexin II lipid-protein complex. [Additions and Corrections]
This article has been retracted by the publisher. An investigation by the Office of Research Integrity determined that falsified and/or fabricated Western blots were included in Figs. 5 and 7 (https://federalregister.gov/a/2012-28209).
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Uittenbogaard, A., Everson, W. V., Matveev, S. V., Smart, E. J. Tags: Additions and Corrections Source Type: research

Palmitoylation of caveolin-1 is required for cholesterol binding, chaperone complex formation, and rapid transport of cholesterol to caveolae. [Additions and Corrections]
This article has been retracted by the publisher. An investigation by the Office of Research Integrity determined that falsified and/or fabricated Western blots were included in Fig. 2A (https://federalregister.gov/a/2012-28209).
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Uittenbogaard, A., Smart, E. J. Tags: Additions and Corrections Source Type: research

Hypercholesterolemia promotes a CD36-dependent and endothelial nitric-oxide synthase-mediated vascular dysfunction. [Additions and Corrections]
This article has been retracted by the publisher. An investigation by the Office of Research Integrity determined that falsified and/or fabricated Western blots were included in Figs. 2 (A–C) and 4 (A and B) (https://federalregister.gov/a/2012-28209).
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Kincer, J. F., Uittenbogaard, A., Dressman, J., Guerin, T. M., Febbraio, M., Guo, L., Smart, E. J. Tags: Additions and Corrections Source Type: research

Myristic acid stimulates endothelial nitric-oxide synthase in a CD36- and an AMP kinase-dependent manner. [Additions and Corrections]
This article has been retracted by the publisher. An investigation by the Office of Research Integrity determined that falsified and/or fabricated Western blots were included in Figs. 1A and 5B (https://federalregister.gov/a/2012-28209).
Source: Journal of Biological Chemistry - March 1, 2013 Category: Chemistry Authors: Zhu, W., Smart, E. J. Tags: Additions and Corrections Source Type: research