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This page shows you the most recent publications within this specialty of the MedWorm directory.

Sorafenib and HDAC inhibitors synergize with TRAIL to kill tumor cells
Abstract The present studies were designed to compare and contrast the abilities of TRAIL (death receptor agonist) and obatoclax (BCL‐2 family inhibitor) to enhance sorafenib + HDAC inhibitor toxicity in GI tumor cells. Sorafenib and HDAC inhibitor treatment required expression of CD95 to kill GI tumor cells in vitro and in vivo. In cells lacking CD95 expression, TRAIL treatment, and to a lesser extent obatoclax, enhanced the lethal effects of sorafenib + HDAC inhibitor exposure. In hepatoma cells expressing CD95 a similar data pattern emerged with respect to the actions of TRAIL. Downstream of the death receptor...
Source: Journal of Cellular Physiology - May 18, 2013 Category: Cytology Authors: Hossein A. Hamed, Yukihiro Yamaguchi, Paul B. Fisher, Steven Grant, Paul Dent Tags: Original Research Article Source Type: research

Multiple genetic pathways regulate replicative senescence in telomerase‐deficient yeast
This article is protected by copyright. All rights reserved. (Source: Aging Cell)
Source: Aging Cell - May 18, 2013 Category: Cytology Authors: Bari J. Ballew, Victoria Lundblad Tags: Original Article Source Type: research

Survival and differentiation defects contribute to neutropenia in glucose-6-phosphatase-β (G6PC3) deficiency in a model of mouse neutrophil granulocyte differentiation
Survival and differentiation defects contribute to neutropenia in glucose-6-phosphatase-β (G6PC3) deficiency in a model of mouse neutrophil granulocyte differentiation Cell Death and Differentiation advance online publication, May 17 2013. doi:10.1038/cdd.2013.39 Authors: S Gautam, S Kirschnek, I E Gentle, C Kopiniok, P Henneke, H Häcker, L Malleret, A Belaaouaj & G Häcker (Source: Cell Death and Differentiation)
Source: Cell Death and Differentiation - May 17, 2013 Category: Cytology Authors: S GautamS KirschnekI E GentleC KopiniokP HennekeH HäckerL MalleretA BelaaouajG Häcker Tags: neutropenia apoptosis differentiation Source Type: research

Interaction between the TP63 and SHH pathways is an important determinant of epidermal homeostasis
Toftgard & T J McDonnell (Source: Cell Death and Differentiation)
Source: Cell Death and Differentiation - May 17, 2013 Category: Cytology Authors: N S ChariR A RomanoM I KosterV JaksD RoopE R FloresS TeglundS SinhaW GruberF AbergerL J MedeirosR ToftgardT J McDonnell Tags: SUFU TP63 BCL2 keratinocyte differentiation non-melanoma skin cancer Source Type: research

Disease-relevant proteostasis regulation of cystic fibrosis transmembrane conductance regulator
Raia, G Kroemer & L Maiuri (Source: Cell Death and Differentiation)
Source: Cell Death and Differentiation - May 17, 2013 Category: Cytology Authors: V R VillellaS EspositoE M BrusciaM VicinanzaS CenciS GuidoM Pettoello-MantovaniR CarnuccioM A De MatteisA LuiniM C MaiuriV RaiaG KroemerL Maiuri Tags: CFTR conformational diseases protein trafficking proteostasis SQSTM1 Source Type: research

Danger signalling during cancer cell death: origins, plasticity and regulation
& P Agostinis (Source: Cell Death and Differentiation)
Source: Cell Death and Differentiation - May 17, 2013 Category: Cytology Authors: A D GargS MartinJ GolabP Agostinis Tags: immunogenic cell death DAMPs danger signals cancer immunogenicity tumour immunology anti-tumour immunity Source Type: research

Evidence for a common progenitor of epithelial and mesenchymal components of the liver
, P Bianco & M Tripodi (Source: Cell Death and Differentiation)
Source: Cell Death and Differentiation - May 17, 2013 Category: Cytology Authors: A ConigliaroL AmiconeV CostaM De Santis PuzzoniaC ManconeB SacchettiC CicchiniF GaribaldiD A BrennerT KisselevaP BiancoM Tripodi Tags: liver stem cell hepatic stellate cell cell transplantation differentiation Source Type: research

[Perspective] Cell Biology: Insulin Finds Its Niche
Localized insulin signaling allows organ-specific rather than organism-level responses to the environmental conditions.Authors: Seth W. Cheetham, Andrea H. Brand (Source: Science: Current Issue)
Source: Science: Current Issue - May 16, 2013 Category: Science Authors: Seth W. Cheetham Source Type: research

Detection of high risk human papillomavirus by hybrid capture II(r) according cytological findings in women treated for squamous intraepithelial lesions of the cervix, period 2006/2010
CONCLUSION: La detección de HR-HPV por CH II(r), así como los valores de carga viral relativa altos, en especial en mujeres con NSIL podrían ayudar a identificar mujeres tratadas con riesgo a desarrollar recidivas, contribuyendo así a fortalecer el programa de prevención de cáncer de cuello uterino. OBJECTIVE: To determinate the frequency of high risk human papillomavirus (HR-HPV) by hybrid capture II (r) (CH II(r)), according cytology results in women treated for squamous intraepithelial lesions of the cervix (SIL). MATERIAL AND METHODS: A descriptive cross-sectional study ...
Source: Revista Brasileira de Epidemiologia - May 16, 2013 Category: Epidemiology Source Type: research

Declining signal dependence of Nrf2‐MafS‐regulated gene expression correlates with aging phenotypes
Summary Aging is a degenerative process characterized by declining molecular, cell and organ functions, and accompanied by the progressive accumulation of oxidatively damaged macromolecules. This increased oxidative damage may be causally related to an age‐associated dysfunction of defense mechanisms, which effectively protect young individuals from oxidative insults. Consistently, older organisms are more sensitive to acute oxidative stress exposures than young ones. In studies on the Drosophila Nrf2 transcription factor CncC, we have investigated possible causes for this loss of stress resistance and its connection to ...
Source: Aging Cell - May 16, 2013 Category: Cytology Authors: Mohammed Mahidur Rahman, Gerasimos P. Sykiotis, Mayuko Nishimura, Rolf Bodmer, Dirk Bohmann Tags: Original Article Source Type: research

Cell cycle-dependent localization of CHK2 at centrosomes during mitosis
Conclusion: Our findings demonstrate that a subpopulation of CHK2 localizes at the centrosomes in mitotic cells but not in interphase. These results are consistent with previous reports supporting a role for CHK2 in the bipolar spindle formation and the timely progression of mitosis. (Source: Cell Division)
Source: Cell Division - May 16, 2013 Category: Cytology Authors: Guillaume ChouinardIsabelle ClémentJulie LafontaineFrancis RodierEstelle Schmitt Source Type: research

Mutations in the Intellectual Disability Gene Ube2a Cause Neuronal Dysfunction and Impair Parkin-Dependent Mitophagy
Dominik M. Haddad, Sven Vilain, Melissa Vos, Giovanni Esposito, Samer Matta, Vera M. Kalscheuer, Katleen Craessaerts, Maarten Leyssen, Rafaella M.P. Nascimento, Angela M. Vianna-Morgante, Bart De Strooper, Hilde Van Esch, Vanessa A. Morais, Patrik Verstreken. The prevalence of intellectual disability is around 3%; however, the etiology of the disease remains unclear in most cases. We identified a series of patients with X-linked intellectual disability.... (Source: Molecular Cell)
Source: Molecular Cell - May 15, 2013 Category: Cytology Source Type: research

A Posttranslational Modification Cascade Involving p38, Tip60, and PRAK Mediates Oncogene-Induced Senescence
Hui Zheng, Alim Seit-Nebi, Xuemei Han, Aaron Aslanian, John Tat, Rong Liao, John R. Yates, Peiqing Sun. Oncogene-induced senescence is an important tumor-suppressing defense mechanism. However, relatively little is known about the signaling pathway mediating the senescence response. Here, we demonst.... (Source: Molecular Cell)
Source: Molecular Cell - May 15, 2013 Category: Cytology Source Type: research

Feedforward Regulation Ensures Stability and Rapid Reversibility of a Cellular State
Andreas Doncic, Jan M. Skotheim. Cellular transitions are important for all life. Such transitions, including cell fate decisions, often employ positive feedback regulation to establish and stabilize new cellular states. However,.... (Source: Molecular Cell)
Source: Molecular Cell - May 15, 2013 Category: Cytology Source Type: research

Listerin-Dependent Nascent Protein Ubiquitination Relies on Ribosome Subunit Dissociation
Sichen Shao, Karina von der Malsburg, Ramanujan S. Hegde. Quality control of defective mRNAs relies on their translation to detect the lesion. Aberrant proteins are therefore an obligate byproduct of mRNA surveillance and must be degraded to avoid disrup.... (Source: Molecular Cell)
Source: Molecular Cell - May 15, 2013 Category: Cytology Source Type: research

A pH-Regulated Quality Control Cycle for Surveillance of Secretory Protein Assembly
Stefano Vavassori, Margherita Cortini, Shoji Masui, Sara Sannino, Tiziana Anelli, Imma R. Caserta, Claudio Fagioli, Maria F. Mossuto, Arianna Fornili, Eelco van Anken, Massimo Degano, Kenji Inaba, Roberto Sitia. To warrant the quality of the secretory proteome, stringent control systems operate at the endoplasmic reticulum (ER)-Golgi interface, preventing the release of nonnative products. Incompletely as.... (Source: Molecular Cell)
Source: Molecular Cell - May 15, 2013 Category: Cytology Source Type: research

Fine‐needle aspiration of histoplasmosis in the era of endoscopic ultrasound and endobronchial ultrasound
CONCLUSIONSIn an endemic region, histoplasmosis presents more commonly in immunocompetent patients as localized fibrocaseous disease on FNA and is often identified by high‐resolution imaging. FNA is increasingly used in the diagnosis because of endoscopic ultrasound and endobronchial ultrasound. GMS stain on direct smears is more sensitive than cell block. In general, laboratory tests have low sensitivity in this patient population. Cancer (Cancer Cytopathol) 2013. © 2013 American Cancer Society. (Source: Cancer Cytopathology)
Source: Cancer Cytopathology - May 15, 2013 Category: Pathology Authors: Michael P. Gailey, J. Stacey Klutts, Chris S. Jensen Tags: Original Article Source Type: research

Cell biology: Sperm control DNA breaks
Nature 497, 7449 (2013). doi:10.1038/497291c When dividing to produce mammalian sex cells, cells coordinate how DNA breaks occur.The cell-division process that forms these sex cells, which contain one set of chromosomes instead of the normal two, involves the swapping of bits of genetic material between matching pairs of chromosomes. (Source: Nature)
Source: Nature - May 15, 2013 Category: Research Tags: Research Highlights Source Type: research

Lung cancer screening with low-dose CT: benefits and potential risks
Commentary on: Bach PB, Mirkin JN, Oliver TK, et al.. Benefits and harms of CT screening for lung cancer: a systematic review. JAMA 2012;307:2418–29. Context Lung cancer is the leading cause of cancer death worldwide.1 The majority of patients present with advanced disease and the current 5-year survival is only 15%.2 Previous research showed no mortality benefit to screening with chest radiography and sputum cytology. The recent results of the National Lung Screening Trial (NLST) are the first to show a significant reduction in lung cancer mortality with the use of low-dose CT (LDCT) in high risk individuals. The po...
Source: Evidence-Based Medicine - May 15, 2013 Category: Internal Medicine Authors: Tanner, N. T., Silvestri, G. A. Tags: Smoking and tobacco, Clinical trials (epidemiology), Epidemiologic studies, Stroke, Screening (oncology), Radiology, Clinical diagnostic tests, Radiology (diagnostics), Screening (epidemiology), Guidelines, Health education, Screening (public health), Smo Source Type: research

The mouse ruby‐eye 2d (ru2d/Hps5ru2‐d) allele inhibits eumelanin but not pheomelanin synthesis
This article is protected by copyright. All rights reserved. (Source: Pigment Cell Research)
Source: Pigment Cell Research - May 15, 2013 Category: Cytology Authors: Tomohisa Hirobe, Shosuke Ito, Kazumasa Wakamatsu Tags: Short Communication Source Type: research

T cell immunotherapy for melanoma from bedside to bench to barn and back: how conceptual advances in experimental mouse models can be translated into clinical benefit for patients
Summary A solid scientific basis now supports the concept that cytotoxic T lymphocytes can specifically recognize and destroy melanoma cells. Over the last decades, clinicians and basic scientists have joined forces to advance our concepts of melanoma immunobiology. This has catalyzed the rational development of therapeutic approaches to enforce melanoma‐specific T cell responses. Preclinical studies in experimental mouse models paved the way for their successful translation into clinical benefit for patients with metastatic melanoma. A more thorough understanding of how melanomas develop resistance to T cell immunothera...
Source: Pigment Cell Research - May 15, 2013 Category: Cytology Authors: Thomas Tüting Tags: Perspective Source Type: research

Loss of the Birt–Hogg–Dubé gene product folliculin induces longevity in a hypoxia‐inducible factor–dependent manner
Summary Signaling through the hypoxia‐inducible factor hif‐1 controls longevity, metabolism, and stress resistance in Caenorhabditis elegans. Hypoxia‐inducible factor (HIF) protein levels are regulated through an evolutionarily conserved ubiquitin ligase complex. Mutations in the VHL gene, encoding a core component of this complex, cause a multitumor syndrome and renal cell carcinoma in humans. In the nematode, deficiency in vhl‐1 promotes longevity mediated through HIF‐1 stabilization. However, this longevity assurance pathway is not yet understood. Here, we identify folliculin (FLCN) as a novel interactor of t...
Source: Aging Cell - May 15, 2013 Category: Cytology Authors: Hakam Gharbi, Francesca Fabretti, Puneet Bharill, Markus M. Rinschen, Sibylle Brinkkötter, Peter Frommolt, Volker Burst, Bernhard Schermer, Thomas Benzing, Roman‐Ulrich Müller Tags: Original Article Source Type: research

Classic "broken cell" techniques and newer live cell methods for cell cycle assessment
Many common, important diseases are either caused or exacerbated by hyperactivation (e.g., cancer) or inactivation (e.g., heart failure) of the cell division cycle. A better understanding of the cell cycle is critical for interpreting numerous types of physiological changes in cells. Moreover, new insights into how to control it will facilitate new therapeutics for a variety of diseases and new avenues in regenerative medicine. The progression of cells through the four main phases of their division cycle [G0/G1, S (DNA synthesis), G2, and M (mitosis)] is a highly conserved process orchestrated by several pathways (e.g., tr...
Source: AJP: Cell Physiology - May 15, 2013 Category: Cytology Authors: Henderson, L., Bortone, D. S., Lim, C., Zambon, A. C. Tags: METHODS IN CELL PHYSIOLOGY Source Type: research

Prolyl carboxypeptidase: a forgotten kidney angiotensinase. Focus on "Identification of prolyl carboxypeptidase as an alternative enzyme for processing of renal angiotensin II using mass spectrometry"
(Source: AJP: Cell Physiology)
Source: AJP: Cell Physiology - May 15, 2013 Category: Cytology Authors: Carlos Q. Velez, J. Tags: EDITORIAL FOCUS Source Type: research

Minding the gaps that link intrinsic circadian clock within the heart to its intrinsic ultradian pacemaker clocks. Focus on "The cardiomyocyte molecular clock, regulation of Scn5a, and arrhythmia susceptibility"
(Source: AJP: Cell Physiology)
Source: AJP: Cell Physiology - May 15, 2013 Category: Cytology Authors: Lakatta, E. G., Yaniv, Y., Maltsev, V. A. Tags: EDITORIAL FOCUS Source Type: research

Identification of prolyl carboxypeptidase as an alternative enzyme for processing of renal angiotensin II using mass spectrometry
In conclusion, results suggest that ACE2 metabolizes ANG II in the kidney at neutral and basic pH, while PCP catalyzes the same reaction at acidic pH. This is the first report demonstrating that renal ANG-(1–7) formation from ANG II is independent of ACE2. Elucidation of ACE2-independent ANG-(1–7) production pathways may have clinically important implications in patients with metabolic and renal disease. (Source: AJP: Cell Physiology)
Source: AJP: Cell Physiology - May 15, 2013 Category: Cytology Authors: Grobe, N., Weir, N. M., Leiva, O., Ong, F. S., Bernstein, K. E., Schmaier, A. H., Morris, M., Elased, K. M. Tags: CALL FOR PAPERS Source Type: research

The cardiomyocyte molecular clock, regulation of Scn5a, and arrhythmia susceptibility
The molecular clock mechanism underlies circadian rhythms and is defined by a transcription-translation feedback loop. Bmal1 encodes a core molecular clock transcription factor. Germline Bmal1 knockout mice show a loss of circadian variation in heart rate and blood pressure, and they develop dilated cardiomyopathy. We tested the role of the molecular clock in adult cardiomyocytes by generating mice that allow for the inducible cardiomyocyte-specific deletion of Bmal1 (iCSBmal1). ECG telemetry showed that cardiomyocyte-specific deletion of Bmal1 (iCSBmal1–/–) in adult mice slowed heart rate, prolonged RR and QRS...
Source: AJP: Cell Physiology - May 15, 2013 Category: Cytology Authors: Schroder, E. A., Lefta, M., Zhang, X., Bartos, D., Feng, H.-Z., Zhao, Y., Patwardhan, A., Jin, J.-P., Esser, K. A., Delisle, B. P. Tags: CALL FOR PAPERS Source Type: research

Epithelial morphogenesis of MDCK cells in three-dimensional collagen culture is modulated by interleukin-8
Epithelial morphogenesis is dependent upon a variety of factors, many of which involve complex interactions between cells and their surrounding environments. We analyzed the patterns of differential gene expression associated with Madin-Darby canine kidney (MDCK) renal epithelial cells grown within a collagen gel in three-dimensional (3D) culture compared with those grown atop a collagen gel in two-dimensional (2D) culture. Under these conditions, MDCK cells spontaneously formed either hollow spherical cysts or flat monolayer sheets, respectively. Microarray analysis of gene expression revealed a twofold or greater express...
Source: AJP: Cell Physiology - May 15, 2013 Category: Cytology Authors: Wells, E. K., Yarborough, O., Lifton, R. P., Cantley, L. G., Caplan, M. J. Tags: CALL FOR PAPERS Source Type: research

Vesicular nucleotide transporter regulates the nucleotide content in airway epithelial mucin granules
Nucleotides within the airway surface liquid promote fluid secretion via activation of airway epithelial purinergic receptors. ATP is stored within and released from mucin granules as co-cargo with mucins, but the mechanism by which ATP, and potentially other nucleotides, enter the lumen of mucin granules is not known. We assessed the contribution of the recently identified SLC17A9 vesicle nucleotide transporter (VNUT) to the nucleotide availability within isolated mucin granules and further examined the involvement of VNUT in mucin granule secretion-associated nucleotide release. RT-PCR and Western blot analyses indicated...
Source: AJP: Cell Physiology - May 15, 2013 Category: Cytology Authors: Sesma, J. I., Kreda, S. M., Okada, S. F., van Heusden, C., Moussa, L., Jones, L. C., O'Neal, W. K., Togawa, N., Hiasa, M., Moriyama, Y., Lazarowski, E. R. Tags: ARTICLES Source Type: research

Relative CO2/NH3 selectivities of mammalian aquaporins 0-9
Previous work showed that aquaporin 1 (AQP1), AQP4-M23, and AQP5 each has a characteristic CO2/NH3 and CO2/H2O permeability ratio. The goal of the present study is to characterize AQPs 0–9, which traffic to the plasma membrane when heterologously expressed in Xenopus oocytes. We use video microscopy to compute osmotic water permeability (Pf) and microelectrodes to record transient changes in surface pH (pHS) caused by CO2 or NH3 influx. Subtracting respective values for day-matched, H2O-injected control oocytes yields the channel-specific values Pf* and pHS*. We find that Pf* is significantly >0 for all AQPs teste...
Source: AJP: Cell Physiology - May 15, 2013 Category: Cytology Authors: Geyer, R. R., Musa-Aziz, R., Qin, X., Boron, W. F. Tags: ARTICLES Source Type: research

Myostatin-induced inhibition of the long noncoding RNA Malat1 is associated with decreased myogenesis
Myostatin, a member of the transforming growth factor-β (TGF-β) superfamily of secreted proteins, is a potent negative regulator of myogenesis. Free myostatin induces the phosphorylation of the Smad family of transcription factors, which, in turn, regulates gene expression, via the canonical TGF-β signaling pathway. There is, however, emerging evidence that myostatin can regulate gene expression independent of Smad signaling. As such, we acquired global gene expression data from the gastrocnemius muscle of C57BL/6 mice following a 6-day treatment with recombinant myostatin compared with vehicle-treated anima...
Source: AJP: Cell Physiology - May 15, 2013 Category: Cytology Authors: Watts, R., Johnsen, V. L., Shearer, J., Hittel, D. S. Tags: ARTICLES Source Type: research

Localization and function of Xin{alpha} in mouse skeletal muscle
The Xin repeat-containing proteins were originally found in the intercalated discs of cardiac muscle with implicated roles in cardiac development and function. A pair of paralogous genes, Xinα (Xirp1) and Xinβ (Xirp2), is present in mammals. Ablation of the mouse Xinα (mXinα) did not affect heart development but caused late-onset adulthood cardiac hypertrophy and cardiomyopathy with conductive defects. Both mXinα and mXinβ are also found in the myotendinous junction (MTJ) of skeletal muscle. Here we investigated the structural and functional significance of mXinα in skeletal muscle. ...
Source: AJP: Cell Physiology - May 15, 2013 Category: Cytology Authors: Feng, H.-Z., Wang, Q., Reiter, R. S., Lin, J. L.- C., Lin, J. J.- C., Jin, J.- P. Tags: ARTICLES Source Type: research

On the role of ATP release, ectoATPase activity, and extracellular ADP in the regulatory volume decrease of Huh-7 human hepatoma cells
Hypotonicity triggered in human hepatoma cells (Huh-7) the release of ATP and cell swelling, followed by volume regulatory decrease (RVD). We analyzed how the interaction between those processes modulates cell volume. Cells exposed to hypotonic medium swelled 1.5 times their basal volume. Swelling was followed by 41% RVD40 (extent of RVD after 40 min of maximum), whereas the concentration of extracellular ATP (ATPe) increased 10 times to a maximum value at 15 min. Exogenous apyrase (which removes di- and trinucleotides) did not alter RVD, whereas exogenous Na+-K+-ATPase (which converts ATP to ADP in the extracellular mediu...
Source: AJP: Cell Physiology - May 15, 2013 Category: Cytology Authors: Espelt, M. V., de Tezanos Pinto, F., Alvarez, C. L., Alberti, G. S., Incicco, J., Denis, M. F. L., Davio, C., Schwarzbaum, P. J. Tags: ARTICLES Source Type: research

MIA promotes melanoma development through activation of YBX1.
This article is protected by copyright. All rights reserved. PMID: 23672612 [PubMed - as supplied by publisher] (Source: Pigment Cell and Melanoma Research)
Source: Pigment Cell and Melanoma Research - May 15, 2013 Category: Cytology Authors: Schmid R, Meyer K, Spang R, Schittek B, Bosserhoff AK Tags: Pigment Cell Melanoma Res Source Type: research

The mouse ruby-eye 2(d) (ru2(d) /Hps5(ru2-d) ) allele inhibits eumelanin but not pheomelanin synthesis.
This article is protected by copyright. All rights reserved. PMID: 23672590 [PubMed - as supplied by publisher] (Source: Pigment Cell and Melanoma Research)
Source: Pigment Cell and Melanoma Research - May 15, 2013 Category: Cytology Authors: Hirobe T, Ito S, Wakamatsu K Tags: Pigment Cell Melanoma Res Source Type: research

Regulation of De Novo Ceramide Synthesis: The Role of Dihydroceramide Desaturase and Transcriptional Factors NFATC and Hand2 in the Hypoxic Mouse Heart
DNA and Cell Biology , Vol. 0, No. 0. (Source: DNA and Cell Biology)
Source: DNA and Cell Biology - May 14, 2013 Category: Cytology Tags: article Source Type: research

Cell-Cell Communication between Malaria-Infected Red Blood Cells via Exosome-like Vesicles
Neta Regev-Rudzki, Danny W. Wilson, Teresa G. Carvalho, Xavier Sisquella, Bradley M. Coleman, Melanie Rug, Dejan Bursac, Fiona Angrisano, Michelle Gee, Andrew F. Hill, Jake Baum, Alan F. Cowman. Cell-cell communication is an important mechanism for information exchange promoting cell survival for the control of features such as population density and differentiation. We determined that (Source: Cell)
Source: Cell - May 14, 2013 Category: Cytology Source Type: research

Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer
Masahito Tachibana, Paula Amato, Michelle Sparman, Nuria Marti Gutierrez, Rebecca Tippner-Hedges, Hong Ma, Eunju Kang, Alimujiang Fulati, Hyo-Sang Lee, Hathaitip Sritanaudomchai, Keith Masterson, Janine Larson, Deborah Eaton, Karen Sadler-Fredd, David Battaglia, David Lee, Diana Wu, Jeffrey Jensen, Phillip Patton, Sumita Gokhale, Richard L. Stouffer, Don Wolf, Shoukhrat Mitalipov. Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by somatic cell nuclear transfer (SCNT) has been envisioned as an approach for generating patient-matched nuclear transfer .... (Source: Cell)
Source: Cell - May 14, 2013 Category: Cytology Source Type: research

Endosalpingiosis in peritoneal washings in women with benign gynecologic conditions
CONCLUSIONSEndosalpingiosis in PWs can be diagnostically challenging. Awareness of intraoperative techniques and correlation with surgical biopsy findings are necessary to avoid a misdiagnosis of malignancy. Cancer (Cancer Cytopathol) 2013. © 2013 American Cancer Society. (Source: Cancer Cytopathology)
Source: Cancer Cytopathology - May 14, 2013 Category: Pathology Authors: Nour Sneige, Marilyn A. Dawlett, Teresa L. Kologinczak, Ming Guo Tags: Original Article Source Type: research

AMPK-p53 pathway activates mitochondrial Bak [Cell Biology]
Oncogenic transcription factor Myc deregulates the cell cycle and simultaneously reprograms cellular metabolism to meet the biosynthetic and bioenergetic needs of proliferation. Myc also sensitizes cells to mitochondria-dependent apoptosis. Although metabolic reprogramming has been circumstantially connected to vulnerability to apoptosis, the connecting molecular pathways have remained poorly defined. Here, we... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - May 14, 2013 Category: Science Authors: Nieminen, A. I., Eskelinen, V. M., Haikala, H. M., Tervonen, T. A., Yan, Y., Partanen, J. I., Klefstrom, J. Tags: PNAS Plus Source Type: research

Transdifferentiation of human stem cells [Cell Biology]
We report here the generation of antibody agonists from intracellular combinatorial libraries that transdifferentiate human stem cells. Antibodies that are agonists for the granulocyte colony stimulating factor receptor were selected from intracellular libraries on the basis of their ability to activate signaling pathways in reporter cells. We used a specialized... (Source: Proceedings of the National Academy of Sciences)
Source: Proceedings of the National Academy of Sciences - May 14, 2013 Category: Science Authors: Xie, J., Zhang, H., Yea, K., Lerner, R. A. Tags: Biological Sciences Source Type: research

Phytochemical Genomics on the Way
(Source: Plant and Cell Physiology)
Source: Plant and Cell Physiology - May 14, 2013 Category: Cytology Authors: Muranaka, T., Saito, K. Tags: Editorial Source Type: research

Benzylisoquinoline Alkaloid Metabolism: A Century of Discovery and a Brave New World
Benzylisoquinoline alkaloids (BIAs) are a structurally diverse group of plant specialized metabolites with a long history of investigation. Although the ecophysiological functions of most BIAs are unknown, the medicinal properties of many compounds have been exploited for centuries. These include the narcotic analgesics codeine and morphine, the antimicrobial agents sanguinarine and berberine, and the antitussive and anticancer drug noscapine. BIA biosynthesis involves a restricted number of enzyme types that catalyze landmark coupling reactions and subsequent functional group modifications. A pathogenesis-related (PR)10/B...
Source: Plant and Cell Physiology - May 14, 2013 Category: Cytology Authors: Hagel, J. M., Facchini, P. J. Tags: Special Issue - Review Source Type: research

CathaCyc, a Metabolic Pathway Database Built from Catharanthus roseus RNA-Seq Data
The medicinal plant Madagascar periwinkle (Catharanthus roseus) synthesizes numerous terpenoid indole alkaloids (TIAs), such as the anticancer drugs vinblastine and vincristine. The TIA pathway operates in a complex metabolic network that steers plant growth and survival. Pathway databases and metabolic networks reconstructed from ‘omics’ sequence data can help to discover missing enzymes, study metabolic pathway evolution and, ultimately, engineer metabolic pathways. To date, such databases have mainly been built for model plant species with sequenced genomes. Although genome sequence data are not available fo...
Source: Plant and Cell Physiology - May 14, 2013 Category: Cytology Authors: Van Moerkercke, A., Fabris, M., Pollier, J., Baart, G. J. E., Rombauts, S., Hasnain, G., Rischer, H., Memelink, J., Oksman-Caldentey, K.-M., Goossens, A. Tags: Special Issue - Regular Papers Source Type: research

Coupling Deep Transcriptome Analysis with Untargeted Metabolic Profiling in Ophiorrhiza pumila to Further the Understanding of the Biosynthesis of the Anti-Cancer Alkaloid Camptothecin and Anthraquinones
The Rubiaceae species, Ophiorrhiza pumila, accumulates camptothecin, an anti-cancer alkaloid with a potent DNA topoisomerase I inhibitory activity, as well as anthraquinones that are derived from the combination of the isochorismate and hemiterpenoid pathways. The biosynthesis of these secondary products is active in O. pumila hairy roots yet very low in cell suspension culture. Deep transcriptome analysis was conducted in O. pumila hairy roots and cell suspension cultures using the Illumina platform, yielding a total of 2 Gb of sequence for each sample. We generated a hybrid transcriptome assembly of O. pumila using the I...
Source: Plant and Cell Physiology - May 14, 2013 Category: Cytology Authors: Yamazaki, M., Mochida, K., Asano, T., Nakabayashi, R., Chiba, M., Udomson, N., Yamazaki, Y., Goodenowe, D. B., Sankawa, U., Yoshida, T., Toyoda, A., Totoki, Y., Sakaki, Y., Gongora-Castillo, E., Buell, C. R., Sakurai, T., Saito, K. Tags: Special Issue - Regular Papers Source Type: research

Glycyrrhiza uralensis Transcriptome Landscape and Study of Phytochemicals
Medicinal and industrial properties of phytochemicals (e.g. glycyrrhizin) from the root of Glycyrrhiza uralensis (licorice plant) made it an attractive, multimillion-dollar trade item. Bioengineering is one of the solutions to overcome such high market demand and to protect plants from extinction. Unfortunately, limited genomic information on medicinal plants restricts their research and thus biosynthetic mechanisms of many important phytochemicals are still poorly understood. In this work we utilized the de novo (no reference genome sequence available) assembly of Illumina RNA-Seq data to study the transcriptome of the li...
Source: Plant and Cell Physiology - May 14, 2013 Category: Cytology Authors: Ramilowski, J. A., Sawai, S., Seki, H., Mochida, K., Yoshida, T., Sakurai, T., Muranaka, T., Saito, K., Daub, C. O. Tags: Special Issue - Regular Papers Source Type: research

Systematization of the Protein Sequence Diversity in Enzymes Related to Secondary Metabolic Pathways in Plants, in the Context of Big Data Biology Inspired by the KNApSAcK Motorcycle Database
We examined the enzyme sequence diversity related to secondary metabolism by means of batch-learning self-organizing maps (BL-SOMs). Initially, we constructed a map by using a big data matrix consisting of the frequencies of all possible dipeptides in the protein sequence segments of plants and bacteria. The enzyme sequence diversity of the secondary metabolic pathways was examined by identifying clusters of segments associated with certain enzyme groups in the resulting map. The extent of diversity of 15 secondary metabolic enzyme groups is discussed. Data-intensive approaches such as BL-SOM applied to big data matrices a...
Source: Plant and Cell Physiology - May 14, 2013 Category: Cytology Authors: Ikeda, S., Abe, T., Nakamura, Y., Kibinge, N., Hirai Morita, A., Nakatani, A., Ono, N., Ikemura, T., Nakamura, K., Altaf-Ul-Amin, M., Kanaya, S. Tags: Special Issue - Regular Papers Source Type: research

SS-mPMG and SS-GA: Tools for Finding Pathways and Dynamic Simulation of Metabolic Networks
Metabolomics analysis tools can provide quantitative information on the concentration of metabolites in an organism. In this paper, we propose the minimum pathway model generator tool for simulating the dynamics of metabolite concentrations (SS-mPMG) and a tool for parameter estimation by genetic algorithm (SS-GA). SS-mPMG can extract a subsystem of the metabolic network from the genome-scale pathway maps to reduce the complexity of the simulation model and automatically construct a dynamic simulator to evaluate the experimentally observed behavior of metabolites. Using this tool, we show that stochastic simulation can rep...
Source: Plant and Cell Physiology - May 14, 2013 Category: Cytology Authors: Katsuragi, T., Ono, N., Yasumoto, K., Altaf-Ul-Amin, M., Hirai, M. Y., Sriyudthsak, K., Sawada, Y., Yamashita, Y., Chiba, Y., Onouchi, H., Fujiwara, T., Naito, S., Shiraishi, F., Kanaya, S. Tags: Special Issue - Regular Papers Source Type: research

Combinatorial Biosynthesis of Legume Natural and Rare Triterpenoids in Engineered Yeast
Triterpenoid saponins are a diverse group of specialized (secondary) metabolites with many biological properties. The model legume Medicago truncatula has an interesting profile of triterpenoid saponins from which sapogenins are differentiated into hemolytic and non-hemolytic types according to the position of their functional groups and hemolytic properties. Gene co-expression analysis confirmed the presence of candidate P450s whose gene expression correlated highly with that of β-amyrin synthase (bAS). Among these, we identified CYP716A12 and CYP93E2 as key enzymes in hemolytic and non-hemolytic sapogenin biosynthet...
Source: Plant and Cell Physiology - May 14, 2013 Category: Cytology Authors: Fukushima, E. O., Seki, H., Sawai, S., Suzuki, M., Ohyama, K., Saito, K., Muranaka, T. Tags: Special Issue - Regular Papers Source Type: research