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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 28.

Topically applied γ-linolenic acid restores corneocyte-bound ω-hydroxy ceramides and ameliorates atopic dermatitis-like symptoms in mice
In this study, we examined the effects of topical application of highly purified γ-linolenic acid (GLA)-containing ointment on the depleted ceramide levels and pruritic dermatitis. To induce AD-like symptoms, HR-1 hairless mice were fed a special diet for 8-12 weeks. GLA (certified purity >99%) was mixed with white petrolatum before the use. After dermatitis was established, GLA (5mg/200mg/animal/day) was applied to the skin for 7 days. Special diet-fed mice exhibited AD-like symptoms; including red scaly skin, decreased skin hydration, increased transepidermal water loss, and prolonged scratching. Histological examinatio...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Masanori Fujii, Hiroyuki Matsuda, Takahiro Toda, Susumu Ohya, Takeshi Nabe Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Sphingoid base composition influences normal function, and dictates altered lamellar bilayer function in atopic dermatitis
We demonstrated here first that alterations not only in ceramides, but also in sphingoid base profiles (i.e., sphingosine to sphinganine ratio), occur in the stratum corneum of AD mice, a model (repeated hapten [oxazolone] challenge) that faithfully replicates human AD. We assessed the potential consequences of altered sphingoid base content for barrier dysfunction in AD using unilamellarvesicles (LV), comprised of major stratum corneum lipids (ceramide, free fatty acids, and cholesterol), with either AD (AD-LV) or normal-LV (normal-LV) levels of sphingosine and/or sphinganine, encapsulating carboxyfluorescein. In response...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Nicolas Loiseau, Yasuko Obata, Sam Moradian, Saeko Yoshino, Kenichi Aburai, Kozo Takayama, Kazutami Sakamoto, Peter_M. Elias, Walter_M. Holleran, Yoshikazu Uchida Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Effects of host defense peptides human β-defensins on skin tight junction barrier
Conclusion] In summary, our study implies that hBDs play a role in improving the skin tight junction barrier. The mechanism by which HDPs function remains to be elucidated.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Chanisa Kiatsurayanon, Francois Niyonsaba, Rithee Smithrithee, Toan The Nguyen, Toshihiro Akiyama, Hiroko Ushio, Shigaku Ikeda, Ko Okumura, Hideoki Ogawa Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Regulation of keratinocyte proliferation by podoplanin during cutaneous wound healing in mice
Podoplanin, a transmembranous glycoprotein, is known as a lymphatic endothelial specific marker in physiological condition. Recent evidence indicates that podoplanin is also markedly expressed by keratinocytes at the leading edge of regenerated epidermis during cutaneous wound healing. However, it remains to be elucidated whether podoplanin plays a functional role in keratinocytes during wound healing. We sought to clarify the biological function of podoplanin in keratinocytes, focusing on their proliferation during wound healing. We created full-thickness wound on the dorsal skin of mice and evaluated the expression inten...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Jun Asai, Satoshi Hirakawa, Jun-ichi Sakabe, Tsunao Kishida, Osam Mazda, Tetsumei Urano, Katsue Suzuki-Inoue, Yoshiki Tokura, Norito Katoh Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Local cortisol activation by 11β-hydroxysteroid dehydrogenase 1 promotes inflammatory reaction in keratinocytes
In this study, we investigated the role of 11β-HSD1 in skin inflammation.First, we investigated the dynamics of 11β-HSD1 under various stimuli. We found that 11β-HSD1 expression was increased by proinflammatory cytokines such as IL-1β and TNFα, and by UVB irradiation in normal human epidermal keratinocytes (NHEK). Correlated with increased 11β-HSD1 expression, the amount of cortisol in culture media which was measured by ELISA increased. To investigate the function of increased 11β-HSD1 under proinflammatory cytokines, we next knocked down 11β-HSD1 by transfecting siRNA. Surprisingly, IL-6 and IL-8 production upreg...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Saori Itoi, Mika Tarao, Hiroyuki Murota, Ichiro Katayama Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

PAR-2 activation enhances Nrf2 signaling in keratinocytes
In conclusion, PAR-2 activation induced the production of intracellular ROS and the expression of Nrf2 in keratinocytes and enhancement of Nrf2 by PAR-2 activation could be achieved through ROS-independent pathway.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Ji Y. Kim, Hyo J. Son, Mi R. Kim, Sang H. Oh Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Transcription factor PTTG1 modulates growth and cytokine production of keratinocytes: Implication in the pathophysiology of psoriasis
Ptuitary tumor transforming gene 1 (PTTG1) is a proto-oncogene that encodes a ubiquitously distributed transcription factor functioning in a variety of cellular processes such as cellular growth/differentiation, tumorigenesis or angiogenesis. Recently, we demonstrated that overexpression of PTTG1 in human keratinocytes resulted in growth enhancement and differential inhibition under three-dimentional culture conditions with upregulation of c-Myc, cyclin B1 and CDK1, the factors closely related with cellular growth or tumorigenesis. On the other hand, PTTG1-depletion resulted in growth suppression and downregulation of the ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yosuke Ishitsuka, Yasuhiro Kawachi, Hiroshi Maruyama, Yasuhiro Fujisawa, Shijima Taguchi, Jun-ichi Furuta, Yasuhiro Nakamura, Yoshiyuki Ishii, Fujio Otsuka Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The mitochondrial fusion proteins Mfn1 and Mfn2 are involved in the skin keratinization
This study investigates whether mitochondria are associated with epidermal keratinization. Mitochondrial distribution was not observed to differ by position in the epidermis, according to staining of mitochondrial outer membrane-located protein (Tom20): Mitochondria were mainly located in the perinuclear region of the cytoplasm. Electromicroscopic observation showed no difference of mitochondrial morphology among basal, squamous and granular layers. However, the expression patterns of Mfn1 and Mfn2 were quite different: Mfn1 was expressed in suprabasal keratinocytes, whereas Mfn2 was expressed only in basal keratinocytes. ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Asuka Suto, Riichiro Abe, Nao Saito, Shotaro Suzuki, Yasuyuki Fujita, Hideki Nakamura, Hiroshi Shimizu Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The role of autophagy in epidermal keratinocytes
In this study, autophagy was evaluated by immunohistochemical staining with anti-LC3 antibody, as LC3 is a key component of autophagy and has been used as a marker of autophagy. The results showed that positive late of LC3 increased with clinical stage and tumor size. These findings suggested that autophagy might play an important role in epidermal keratinization and carcinogenesis
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Nagisa Yoshihara, Atsushi Takagi, Takashi Ueno, Shigaku Ikeda Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Depressed PPARα accelerates a vicious cycle between allergic inflammation and barrier dysfunction
Peroxisome proliferator-activated receptors (PPARs) are nuclear hormone receptors and are of three subtypes: PPARα, PPARβ/δ and PPARγ. Activation of PPARs not only has positive effects on permeability barrier homeostasis but also has anti-inflammatory effects. Reduced expression of PPARα in lesional skin in atopic dermatitis (AD) and the preventive and therapeutic effects of a synthetic ligand for PPARα on AD-like dermatitis in a hapten-induced model of AD in mice have been demonstrated. Epidermal levels of expression of PPARα but of neither PPARβ/δ nor PPARγ were reduced in mice with hapten-induced AD. Expressio...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yasuko Adachi, Yutaka Hatano, Takashi Sakai, Sakuhei Fujiwara Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Expression and functional role of Sox9 in human epidermal keratinocytes
In this study, we investigated the expression and putative role of Sox9 in epidermal keratinocyte. Immunohistochemical staining showed that Sox9 is predominantly expressed in the basal layer of normal human skin epidermis, and highly expressed in several skin diseases including psoriasis, basal cell carcinoma, keratoacanthoma and squamous cell carcinoma. In calcium-induced keratinocyte differentiation model, the expression of Sox9 was decreased in a time dependent manner. When Sox9 was overexpressed using a recombinant adenovirus, cell growth was enhanced, while the expression of differentiation-related genes such as loric...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Jeung-Hoon Lee, Ge Shi, Kyung-Cheol Sohn, Zhengjun Li, Dae-Kyoung Choi, Yi-Ming Fan, Yong Hee Nam, Sooyeon Kim, Myung Im, Chang Deok Kim Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

A desquamation paradox of atopic dermatitis–Markedly remaining corneodesmosomes despite the increased serine protease activity in vitro
Desmosomes in the stratum corneum (SC), also known as corneodesmosomes (CDs), are crucial for maintaining SC integrity and skin barrier function. Localization of CD components can be visualized by immunostaining tape-stripped corneocytes. In our research using this method, CD components were detected only at the cell periphery in normal basket weave-like SC, while diffuse localization was detected in lesional atopic dermatitis (AD) skin showing compact hyperkeratosis. With electron microscopy, we found that CDs increased in numbers and were distributed all around the cells in AD corneocytes. We also found that staining of ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Satomi Igawa, Shin Iinuma, Mari Kishibe, Masako Minami-Hori, Masaru Honma, Masamoto Murakami, Akemi Ishida-Yamamoto, Hajime Iizuka Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Podoplanin alters β1-integrin-mediated cell adhesion and initiates terminal differentiation in human epidermal keratinocytes
Conclusion These results suggest that PDPN induces terminal differentiation of HEK via alteration of β1-integrin expression/function. PDPN might play a role in active supply of differentiating keratinocytes into thickened suprabasal layer of hyperproliferating psoriatic epidermis.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Masaru Honma, Mizue Fujii, Hidetoshi Takahashi, Hajime Iizuka Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

An attempt to identify antigen for IgA pemphigus accidentally showed the presence of transmembrane secretory component in keratinocytes
This study also indicates that TSC may hamper detection of antigens for IgA autoantibodies.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Atsunari Tsuchisaka, Norito Ishii, Hiroshi Koga, Takahiro Hamada, Chika Ohata, Minao Furumura, Takashi Hashimoto Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Decreased TJ Components in Atopic Dermatitis: A Possible Mechanism of Impaired Outside-In Barrier in Atopic Dermatitis
In this study, we examined changes in the TJ barrier in atopic dermatitis associated with a defective outside-in barrier. We then investigated the relationship between the TJ barrier and the outside-in barrier using a human skin equivalent with weakened TJ barrier.An analysis of DNA microarrays in atopic dermatitis showed diminished amounts of claudin-1, -4, -7, -8 and -17 mRNAs. Immunohistochemical and western blot analyses revealed diminished expression of claudin-4 (including claudin-1 shown by De Benedetto et al. (2011)), but no occludin or ZO-1 was observed. Knockdown experiments of claudin-4 in human epidermal kerati...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yuki Takuo, Aya Komiya, Shotaro Ito, Junko Ishikawa, Yoshito Takahashi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Co-localization of kallikrein5 and profilaggrin in keratohyalin granules and reduction of filaggrin monomers by kallikrein5 downmodulation
Kallikrein5 (KLK5) is known as an enzyme involved in epidermal desquamation. We have recently identified KLK5 as one of profilaggrin processing enzymes by LC/MS/MS analysis. However, the localization of KLK5 remains unfully identified in association with profilaggrin, and the functional significance of KLK5 in live keratinocytes needs to be addressed. Here, we sought to demonstrate co-localization of KLK5 and profilaggrin by immunoelectron microscopy and to provide evidence for the functional activity of KLK5 by shRNA analysis. Normal human skin specimens were stained for profilaggrin and KLK5 with rabbit anti-human KLK5 a...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Jun-ichi Sakabe, Isao Ohta, Satoshi Hirakawa, Yoshiki Tokura Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Keratinocyte-specific PRSS3 participates in the desquamation process via degradation of LEKTI
Kallikrein-related peptidases (KLKs) and the lymphoepitherial Kazal-type inhibitor (LEKTI) play important roles in corneocyte desquamation. It is suggested that KLKs such as KLK5 and 7 are released from LEKTI because of acidic pH at the skin surface and degrades corneodesmosomes, resulting in the removal of surface corneocytes. It is still obscure whether the KLK-LEKTI interaction is easily broke in such a weak acidic pH(5.5-6.5). Recently we reported cloning of a new PRSS3 gene product, keratinocyte-specific mesotrypsin from the cDNA library. The aim of this study is to elucidate involvement of PRSS3 in the desquamation p...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Masashi Miyai, Haruyo Yamanishi, Yuuko Matsumoto, Mami Yamamoto, Toshihiko Hibino Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Phospholipase Cδ1 is required for normal expression of profilaggrin in keratinocytes
Phospholipase C (PLC) is a key enzyme in phosphoinositide turnover. Since one of PLC isozymes, PLCδ1 is abundantly expressed in epidermis, we examined expression patterns of differentiation markers in epidermis of mice lacking PLCδ1 (PLCδ1 KO mice). Immunofluorescence revealed that the expression pattern of filaggrin was disturbed in epidermis of PLCδ1 KO mice. siRNA-mediated knockdown of PLCδ1 decreased profilaggrin mRNA in HaCaT cells and normal human keratinocytes. In addition, overexpression of PLCδ1 resulted in significant increase in profilaggrin mRNA in HaCaT cells. Loss-of-function mutations of filaggrin are ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yoshikazu Nakamura, Kaori Kanemaru, Kenji Kabashima, Kyoko Nakahigashi, Kiyoko Fukami Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The p75 neurotrophin receptor triggers keratinocyte stem-transit amplifying cell transition in normal human epidermis
p75 neurotrophin (NT) receptor (p75NTR) can signal independently from the high affinity receptor Trk and mediates apoptosis in human keratinocytes, upon binding with specific ligands. As p75NTR is predominantly expressed in transit amplifying (TA) cells, we aimed to study the role of p75NTR in keratinocyte differentiation. p75NTR was up-regulated during keratinocyte differentiation in cell culture with both serum and calcium treatment. When p75NTR was silenced, calcium treatment failed to induce differentiation in subconfluent keratinocytes. p75NTR positive (p75NTRpos) human keratinocytes, isolated by cell sorting, were le...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Alessandra MArconi, Francesca Truzzi, Roberta Lotti, Tiziana Petrachi, Annalisa Saltari, Carlo Pincelli Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Diagnosis of Idiopathic dirty neck syndrome in Korean women
In conclusion, we propose this dirty neck appearance without associated underlying diseases or systemic diseases including atopic dermatitis, acanthosis nigricance, connective tissue diseases as “idiopathic dirty neck syndrome”. Here, we present a case series with various clinical manifestations of “idiopathic dirty neck syndrome” in Korean women.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Jeong Eun Kim, Joo-Yeon Ko, Sangmin Lee, Hyun Woo Lee, Young Suck Ro, Sung Eun Chang Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

An attempt to calibrate clinician assessment of photoaging through an understanding of subject self assessment
Project Overview Two of the most commonly used procedures for assessing efficacy of photoaging treatments are objective scoring by an expert clinician or by subject self assessment.Based on anecdotal evidence from past studies a hypothesis was generated that test subjects have a different perception of photodamage than the clinician.The scope of this initial investigation was limited to crows foot area lines and wrinkles. Subjects were first asked to describe photos obtained from the JSCS showing mild and severe photodamage. The terminology that was used by the subjects to describe the type and severity of photodamage was...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Jesse C. Leverett, Mangalam Subramanian, Chithra Ganesan, Elvin Chun, Monya Sigler Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Cytomegalovirus infection is one of the precipitating factors for pustular psoriasis
We have an experience with two cases of psoriasis vulagris with cytomegalovirus (CMV) infection followed by generalized pustular psoriasis. Two Japanese males who had been suffered from psoriasis vulgaris developed CMV hepatitis. CMV antigenemia tests revealed CMV infected polymorphonuclear leukocytes in their blood. Diagnosis of generalized pustular psoriasis was made because histological examinations revealed subcorneal spongiform pustules with abundant neutrophils. In order to clarify the missing link between CMV infection and pustular psoriasis, we conducted several experiments. Neutrophils underneath the stratum corne...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kozo Yoneda, Yoshie Matsuoka-Shirahige, Toshio Demitsu, Yasuo Kubota Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Analysis allergic reactions in two patients with anaphylaxis induced by the ingestion of shiitake mushroom
In conclusion, shiitake may cause immediate IgE-mediated allergy that is manifested in the skin as urticaria. This skin symptom is different fromshiitake dermatitis that is caused by eating raw shiitake. Skin tests and immunoblot for immediate allergy and detection of specific IgE are important when contact dermatitis from shiitake is investigated.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kentaro Ohko, Masaaki Ito Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Optical Diagnosis of Atopic Dermatitis with Mental Stress by NIRS (Near-InfraRed Spectroscopy)
In this study, we evaluated 30 atopic dermatitis patients (13 male and 17 female, the median age: 35.1) in our hospital with SCORAD, serum IgE and TARC, PSS-AD, SDS, and NIRS. As a result, for the pattern of NIRS, 20 patients showed normal pattern, 6 patients with depression pattern, and 4 patients with other patterns. The mean score of SCORAD, PSS-AD, SDS, IgE, TARC of each group were normal pattern: 50.1, 33.6, 41.7, 6816.1, 4832.1, depression pattern: 46.8, 32.7, 43 .2, 3426.9, 3042.0, others: 40.6, 42.5, 54.8, 2197.5, 1603.5, respectively. Three of these 6 patients with depression pattern did not enough recognize their...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Eiichiro Ueda, Shinichi Moriwaki, Yoshikazu Fukui, Tetsufumi Kanazawa, Hiroshi Yoneda Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Quantitative analysis of the facial skin microbiota in Japanese acne patients
Background: Many studies on Propionibacterium acnes (P. acnes) in acne patients have been performed, however, the quantitative analysis of the facial skin microbiota of acne patients have rarely been conducted.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Shigeki Numata, Hirohiko Akamatsu, Narifumi Akaza, Yasuyuki Sasaki, Shiori Takeoka, Shunji Yamada, Akiko Yagami, Satoru Nakata, Kayoko Matsunaga Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Withdrawn
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The evaluation of salivary cortisol levels in adult patients with atopic dermatitis
This study, measured salivary samples for cortisol in patients with AD (n=25) and compared them with healthy control subjects (n=42). AD patients were also evaluated for general disease severity using the SCORingAD (SCORAD) score. The serum TARC level, serum total IgE level, serum lactate dehydrogenase (LDH) level, and peripheral blood eosinophil count were measured by laboratory tests. The Skindex-16 was used as a skin disease-specific instrument. The results showed saliva cortisol level was significantly increased in AD patients in comparison to healthy subjects (p
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Megumi Furuichi, Masaki Yamaguchi, Chieko Ueda, Takako Yamakoshi, Teruhiko Makino, Tadamichi Shimizu Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

FOXP3+T cell subset characterized by Helios, FOXP3 and CD45RO in patients with melanoma
Human CD4+FOXP3+T cells in peripheral blood mononuclear cells (PBMCs) are separated into three subsets according to the function and cell-surface phenotype as CD45RO+FOXP3low nonregulatory T cells, CD45RO+FOXP3high effector Tregs, and CD45RO-FOXP3low naive Tregs. We previously reported that all the FOXP3+ T cell subsets were increased in PBMCs from patients with melanoma compared with age-matched healthy controls. It remains elusive how these cells increase and where they originate. Recently, Helios, an Ikaros family of transcription factors, is recognized as a new marker for FOXP3+ T cells. Helios divides FOXP3+ T cells i...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hiroko Fujii, Takashi Nomura, Yoshiki Miyachi, Miki Tanioka Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Immunohistochemical analysis of O6-methylguanine-DNA methyltransferase in melanoma
Alkylating agents, often used for cancer chemotherapy in melanoma and brain tumors, can produce various kinds of adducts to the bases in DNA. Among them, O6-alkylguanine (O6AG) has been supposed to be the major lesion related to tumor cell killing after the treatment of alkylating agents. O6AG is effectively eliminated by a DNA repair enzyme;O6- methylguanine-DNA methyltransferase (O6MGMT). The level of O 6MGMT in tumors is variable, however, brain tumors and melanomas have lower O6MGMT activity compared to those in other epithelial malignant neoplasms and the its level is correlative to the resistance to alkylating agents...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yasuhito Kokunai, Motomu Tsuji, Yuko Ito, Teruo Kurokawa, Shinichi Moriwaki, Katsunori Otsuki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

TARC is a potential marker for early indication of disease and prediction of disease activity in drug-induced hypersensitivity syndrome
Conclusion: Serum TARC levels may be associated with the initial presentation of DIHS as well as disease activity during the course. Thus, they could be useful as an indicator for early diagnosis and assessment of disease activity in DIHS. CD11c+dendritic cells may be the main source of TARC in patients with DIHS.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kohei Ogawa, Hironori Morito, Ayako Hasegawa, Natsuko Daikoku, Fumi Miyagawa, Aiko Okazaki, Takaya Fukumoto, Nobuhiko Kobayashi, Hideaki Watanabe, Sueki Hirohiko, Masafumi Iijima, Mikiko Tohyama, Koji Hashimoto, Hideo Asada Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Clinical treatments for atopic dermatitis modulate thymic stromal lymphopoietin expression in keratinocytes
Thymic stromal lymphopoietin (TSLP) is overexpressed in lesional keratinocytes in atopic dermatitis (AD). The local increase in TSLP is associated with activation of dendritic cells which trigger the differentiation of naive CD4+T cells into proallergic CD4+Th2 cells. Thus, TSLP is thought to play an important role in the pathogenesis of AD, but the alteration of TSLP expression after treatment has not fully been understood. Here we investigated whether TSLP expression in normal human epidermal keratinocytes (NHEKs) could be affected by common clinical treatments including ultraviolet (UV) A and B, glucocorticosteroid, and...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kazuko Ikeda, Shin Morizane, Yumi Aoyama, Keiji Iwatsuki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Detecting Bartonella Henselae Infection in Brazilian Cat Gonads by Multi-step PCR/Culture and Laser Scanning Confocal Microscopy (LSCM)
Bartonella are a re–emergent bacteria with worldwide distribution that can cause illness ranging from chronic asymptomatic infection to life–threatening disease. B. henselae is the species most frequently associated with human infection and is a zoonotic disease. Pets, especially cats, are the most important reservoirs. Infected cats and humans display a cyclic bacteremia though Bartonellas primary niche is still unknown. There are currently no gold–standard tests to confirm Bartonella infection and false–negative results are frequent even with molecular, microbiological, or serological testing techniques. In our p...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kareem Halim, Paulo E.N.F. Velho, Marina L. Cintra, Steffan M. Nye, Sima Kavand, Marna E. Ericson Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Ustekinumab improved psoriasis without altering T cell immunity: cytokine production, differentiation, T cell receptor repertoire diversity
In conclusion, although the number of patients was limited, the present data suggests that ustekinumab achieved significant clinical improvement in psoriasis without apparent immunosuppression that raises concerns about malignancy and infection. Obviously, further investigations for immunomodulation in biologics with large-scale is expected.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Keiichi Yamanaka, Kenshiro Tsuda, Kimiko Matsubara, Makoto Kondo, Hitoshi Mizutani Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Keratinocyte-derived galectin-7 is a potential component of amyloid fibrils in localized cutaneous amyloidosis
Conclusion: Galectin-7 that have a high content of β-sheet structure in epidermal keratinocytes appears to play an essential role in amyloid formation in primary and secondary LCA.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Koji Ono, Yoshinori Miura, Satoru Harumiya, Eita Fujimoto, Minoru Akiyama, Noriko Fujii, Hiroshi Wachi, Shingo Tajima, Takahiro Satoh Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The distinct autocrine PDGF signals in dermatofibrosarcoma protuberans can aid in differential diagnosis from other mesenchymal tumors
Dermatofibrosarcoma protuberans (DFSP) is an intermediate grade malignancy of subcutis with frequent local recurrences. The histological diagnosis is sometimes difficult to differentiate from other mesenchymal tumors including dermatofibroma (DF) and more serious fibrosarcoma. Last year we reported a novel gene fusion in a DFSP cell occurring at the exon 26 of collagen1α2 (COL1A2) fused in frame with the exon 2 of PDGF–B gene. We also noticed the presence of known chromosomal fusion between COL1A1 and PDGF–B in some other DFSP cases. The constitutive expression of PDGF driven by collagen genes should act as autocrine ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Ikumi Nakamura, Etsuko Okada, Masahito Yasuda, Kiyohito Taira, Osamu Ishikawa, Hiroshi Uezato, Kenzo Takahashi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Antiphospholipid Antibodies in Patients with Cutaneous Polyarteritis Nodosa and Livedo Vasculopathy
In conclusion, IgM anti-PS/PT antibodies were detected more frequently than other antiphospholipid antibodies in CPN patients. It is suggested that IgM anti-PS/PT antibodies might act as a pathogenic factor for CPN, and that there might be a common pathogenic mechanism between CPN and some cases of LV, because IgM anti-PS/PT antibodies were also detected in about 40% of LV patients.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Naoko Ishiguro, Natsuko Wakabayashi, Hisashi Yamanaka, Satoru Shimizu, Makoto Kawashima Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

AN2728 and AN2898 Ointments Demonstrate Safety and Efficacy in a Bilateral Study of Atopic Dermatitis
This study was design to determine their safety and efficacy in the treatment of mild-to-moderate atopic dermatitis.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Lee Zane, Tatiana Gogoleva, Frederic Heerinckx, John Jermano Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The inhibitory effects of gentian violet on STAT6 signaling shed light on its classic yet modern therapeutic use for prurigo nodularis
Although the pathomechanism of prurigo nodularis (PN) remains to be determined, we have reported that PN is accompanied by STAT6 and STAT3 activation, which suggests that STAT6 signaling can be a therapeutic target for PN. In addition, we have published that the increased expression of DUOX1, one of NADPH oxidases (NOXs), in IL-4/IL-13-treated NHEK augments STAT6 phosphorylation via oxidative inactivation of protein tyrosine phosphatase 1B, which implies the suppression of DUOX1 as a possible modality to suppress STAT6 signaling. Recently, Perry et al have reported that gentian violet with chemical similarity to diphenylio...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Sawako Fukushi, Yumiko Ito, Yutaka Kimura, Setsuya Aiba Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Oral administration of collagen tripeptide improves dryness and pruritus in the acetone-induced dry skin model
This study was aimed at investigating the therapeutic effects of CTP for dryness and pruitus in acetone-induced dry skin model mice.ICR mice were treated with acetone followed by oral administration of CTP (80 or 500mg/kg/day) for 3 days. As a result, oral administration of CTP in acetone-induced dry skin model mice significantly decreased Transepidermal water loss (TEWL) and suppressed scratching behavior. Furthermore, the effects of CTP on intraepidermal nerve fibers and expressions of semaphorin 3A (Sema 3A) and nerve growth factor (NGF) were examined by immunohistochemistry and quantitative RT-PCR. Intraepidermal nerve...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Tomoko Okawa, Yukie Yamaguchi, Shinnosuke Takada, Yasuo Sakai, Noriaki Numata, Fumio Nakamura, Yoji Nakamura, Zenro Nakamura, Michiko Aihara Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

A Clinical study of influenza vaccine using a dissolving microneedle array
Conclusions: These findings suggest that HA-containing dissolving microneedle arrays can simply, safely, and effectively improve protective immune responses for influenza vaccination.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hiroaki Azukizawa, Sachiko Hirobe, Takaaki Hanafusa, Kazuhiko Matsuo, Ying-Shu Quan, Fumio Kamiyama, Ichiro Katayama, Naoki Okada, Shinsaku Nakagawa Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Psoriatic activity correlated with the increase in Foxp3+regulatory T cells in psoriatic lesions
We examined the skin lesions of 5 other psoriasis patients (4 men, 1 woman) before and after bath-PUVA therapy. Bath-PUVA therapy reduced the number of Foxp3+T cells in the lesions and increased circulating Treg. The induced Treg had substantially restored function. A recent in vitro study revealed a cell population that differentiates into helper T cells from Foxp3+T cells, which develop after transient Foxp3 expression induced by cytokine signals and other environmental factors. Foxp3+T cells have inhibitory activity in human psoriatic lesions, and might also develop into helper T cells, like a double-edged sword. Based ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Emi Nishida, Saori Kasuya, Chiyo Saito, Takuya Furuhashi, Akiko Nishioka, Yoichi Shintani, Akimichi Morita Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Involvement of sweating disturbance in the pathogenesis of lichen planus and atopic dermatitis
Although recent studies indicate that various patterns of sweating disturbance can be observed in inflammatory skin diseases, much less attention has been focused on the role of sweating disturbance in the development of these diseases. To test this, we have established a useful method, the impression mold technique (IMT), which allows an accurate quantification of each sweat gland actively delivering sweat and the volume of sweat it produces. IMT provides an assessment of individual sweat droplet size and number, repeatedly over time. Sweating was induced by either immersing feet for 30min in a water bath, or injecting ac...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yurie Komatsu, Yukiko Ushigome, Takaaki Doi, Yohei Sato, Yoshiko Mizukawa, Tetsuo Shiohara Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The cathelicidin (hCAP-18/LL-37) expression in the granules of leukocytes in the pustules and perhipheral blood with palmoplantar pustulosis
Palmoplantar pustulosis (PPP) is a chronic recurrent dermatosis characterized by intraepidermal vesicles filled with neutrophils. We have shown and reported that PPP vesicle before pustule formation was originated from eccrine sweat in the acrosyringium, and also shown no hCAP-18/LL-37 expression in the keratinocyte around the vesicles (JID 2010). We hypothesized that a malfunction of antimicrobial peptide processing in polymorphonuclear leukocytes (PMN) in pustules may contribute to the pathophysiology of PPP because the abnormal enzymatic processing of cathelicidins by proteinases KLK-5 or KLK-7 in rosacea results in pro...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Masamoto Murakami, Hidenori Okazaki, Yasushi Hanakawa, Mikiko Tohyama, Yuji Shirakata, Koji Sayama Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Comparison of Foxp3+regulatoryT cells and CD163+macrophages in invasive and non invasive extramammary Paget disease
An imbalance of immunosuppressive cells and immunomodulatory cells plays a important role in inhibiting the antitumor immune response of the tumor bearing host. Among such cells, regulatory T cells (Tregs), together with immunosuppressive macrophages such as CD163+M2 macrophages, play roles in maintaining the tumor microenvironment. In addition, MMP9 is a stromal factor that remodels the extracellular matrix and promotes the sprouting and growth of new blood vessels by making VEGF. Recently, MMP9 was found to corelate with immunosuppressive macrophages, such as myeloid derived suppressor cells. The purpose of this study wa...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Sadanori Furudate, Taku Fujimura, Yumi Kambayashi, Akira Hashimoto, Takahiro Haga, Setsuya Aiba Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Comparison of immunosuppressive cells and immunomodulatory cells in Angiosarcoma; the possible novel supportive therapies for angiosarcoma
An imbalance of immunosuppressive cells and immunomodulatory cells plays a important role in inhibiting the antitumor immune response of the tumor-bearing host. Regulatory T cells (Tregs), together with immunosuppressive macrophages, play roles in maintaining the tumor microenvironment. In addition, MMP-9 is a stromal factor that remodels the extracellular matrix and promotes the sprouting and growth of new blood vessels by inducing VEGF. Recently, MMP-9 was found to co-relate with immunosuppressive macrophages, such as myeloid derived suppressor cells (MDSC). In addition, we previously reported that about 80% of cutaneous...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yumi Kambayashi, Taku Fujimura, Sadanori Furudate, Akira Hashimoto, Takahiro Haga, Setsuya Aiba Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Anti-lysosomal-associated membrane protein-2 antibody levels on cutaneous vasculitis
Conclusion: We suggested that the anti-LAMP-2 antibody could play an important role in the pathogenesis of cutaneous vasculitis.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Tamihiro Kawakami, Sora Takeuchi, Satoko Kimura, Yoshinao Soma, Akihiro Ishizu Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Decreased serum LL-37 and vitamin D3 levels in atopic dermatitis: relationship between IL-31 and oncostatin M
This study aimed to examine the serum levels of LL-37 and vitamin D3 and their regulation of cytokine production in patients with AD.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Naoko Kanda, Carren S. Hau, Yayoi Tada, Shinichi Sato, Shinichi Watanabe Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Novel nucleoside reverse transcriptase inhibitor (4’-Ethynyl-2-Fluoro-2’-Deoxyadenosine) completely blocks HIV Infection of Langerhans Cells
Because of the absence of a prophylactic vaccine, new strategies that reduce sexual transmission of HIV are urgently needed. A recent clinical trial has shown that vaginal application of tenofovir (TDF); a nucleotide reverse transcriptase inhibitor (NRTI), greatly reduces the probability of sexual transmission of HIV-1 (Karim QA et al. Science, 2010). To deliver and demonstrate more potent anti-HIV activity of microbicides and long half-life within target tissues such as genital skin and mucosa, we have recently developed a novel NRTI; 4’-Ethynyl-2-Fluoro-2’-Deoxyadenosine (EFdA). Because Langerhans cells (LCs) are sus...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Takamitsu Matsuzawa, Tatsuyoshi Kawamura, Youichi Ogawa, Andrew Blauvelt, Hiroaki Mitsuya, Shinji Shimada Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Langerhans cells are selectively depleted in pellagra skin: the niacin deficiency as immuno-pathogenesis of one more trophic skin disorders
Pellagra is caused by niacin deficiency and characterized by photosensitive dermatitis, diarrhea, dementia, however, the exact pathomechanism of these symptoms is not yet known. Since the pathological and clinical anomalies of pellagre are mostly common to those of zinc defiency, we suspected the alteration of Langerhans cells might be the clue of pellagra lesion, whose disappearance is also noted in acrodermatitis enteropathica recently. We analysed the presence of various cells in pellagra patients, and confirmed the selective disappearance of dendritic cells componens assumed by the immunohistochemical staining for lang...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Sayaka Yamaguchi, Kiyohito Taira, Kenzo Takahashi, Hiroshi Uezato Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research