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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 32.

Synthetic innate defense regulator peptides modulate various functions of human neutrophils
[Introduction] Our skin is continuously exposed to potential pathogens and plays a crucial role in initiating immune responses to eliminate these microorganisms via various mechanisms, including production of host defense peptides (HDPs). Although originally viewed as antimicrobials, HDPs broadly modulate the immune response through activation of different cell types. We have recently developed a series of novel synthetic peptides termed innate defense regulators (IDRs) based on a natural HDP, bactenecin, and showed that both IDR-1 and IDR-1002 protect the host against bacterial infections through chemokine induction. The ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Francois Niyonsaba, Laurence Madera, Ko Okumura, Hideoki Ogawa, Robert E.W. Hancock Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Cathelicidin antimicrobial peptide LL-37 augments anti-viral activity induced by double-stranded RNA in keratinocytes
Cathelicidin antimicrobial peptide LL–37 not only has the capacity to kill a wide variety of microbes, but also can modify host immune and growth responses. Recently our group observed that the activation of keratinocytes by LL–37 and DNA also greatly increases type I IFN through TLR9. On the other hand, another group reported LL–37 inhibit poly (I:C) (TLR3 ligands)–induced immune responses in macrophages. Therefore we investigated whether LL–37 could affect TLR3 signaling in normal human epidermal keratinocytes (NHEKs). Real–time quantitative PCR (RT–qPCR) and ELISA revealed that co–stimulation with LL–3...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Tetsuya Takiguchi, Shin Morizane, Takenobu Yamamoto, Yumi Aoyama, Keiji Iwatsuki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Interferon gamma enhances anti-viral activity induced by double-stranded RNA in keratinocytes
Synthetic double-stranded RNA poly (I:C) are reported to induce several cytokines, chemokines, and antimicrobial peptides through Toll-like receptor 3 (TLR3), RNA helicases retinoic acid-inducible gene I (RIG-I), and Melanoma differentiation-associated protein 5 (MDA5) in keratinocytes. These mechanisms are considered to play important roles in viral skin infection. However, the regulation of these molecules expression and the functions in keratinocytes are not fully understood. We first confirmed high TLR3 expression in keratinocytes surrounding vesicles of herpes zoster by immunohistochemistry. We also investigated facto...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Ai Kajita, Shin Morizane, Takenobu Yamamoto, Kenshi Yamasaki, Yumi Aoyama, Keiji Iwatsuki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Increased expression of PILRα on patrolling monocytes sensing HSV is the mechanism by which HSV evades immune attack
HSV-1 and HSV-2 utilizes several glycoprotein-receptor complexes when entering into host cells. Recently, PILRα has been identified as a glycoprotein B receptor for HSV and PILRα was preferentially expressed on monocytes (MO). However, because MOs are composed of at least three different subpopulations, CD14++CD16–MOs, CD14+CD16+ MOs and CD14dimCD16+ MOs, it remains to be determined which subpopulations preferentially express PILRα sensing herpesvirus. Among these subpopulations, CD14dimCD16+ MOs have recently received increasing attention, because this population has been shown to patrol blood vessels and selectively...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Ryo Takahashi, Tetsuo Shiohara Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Host defense peptides, human β-defensins increase interleukin-37 production in human keratinocytes
In conclusion, above observations suggest that hBDs may contribute to the regulation of skin inflammation through their capacity to induce IL-37 expression by keratinocytes.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Rithee Smithrithee, Francois Niyonsaba, Chanisa Kiatsurayanon, Toan The Nguyen, Toshihiro Akiyama, Hiroko Ushio, Shigaku Ikeda, Ko Okumura, Hideoki Ogawa Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

A reactivation signal, BZLF1 expression, is a prognostic marker for severe hydroa vacciniforme and hypersensitivity to mosquito bites
Epstein-Barr virus (EBV)-associated T/NK lymphoproliferative disorders (LPDs) are a group of diseases including typical and severe hydroa vacciniforme (HV) and hypersensitivity to mosquito bites (HMB). Apart from typical HV, many patients with severe HV and HMB result in a fatal outcome. However, little has been known about the cellular and molecular events leading to the poor prognosis. The present study was designed to clarify molecular markers related to the prognosis of EBV-associated T/NK LPDs. A total of 52 patients were categorized into 4 groups by clinicopathologic criteria: typical HV, severe HV, HMB, and HMB+HV. ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Tomoko Miyake, Takenobu Yamamoto, Yoji Hirai, Keiji Iwatsuki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Preferential elimination of patrolling monocytes sensing herpesvirus in drug-induced hypersensitivity syndrome (DIHS)
In this study, we sought to determine whether the dynamics of the ‘patrolling’ MO could contribute to the evolution process of severe drug eruption. In Stevens-Johnson syndrome(SJS)/toxic epidermal necrolysis(TEN), in which herpesvirus reactivation is not observed and epidermal structures are specifically damaged, PILR+CD14dimCD16+MO were abundantly detected in the damaged epidermis. In PBL, they were identified in normal frequency. In contrast, at the early stage of DIHS, this population was rarely identified in the PBL and epidermis. Upon recovery, they gradually increased to normal frequency. Such preferential elimi...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yukiko Ushigome, Ryo takahashi, Tetsuo Shiohara Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Ataxia telangiectasia patients cannot cause severe Herpes Simplex because of disturbed Herpes simplex virus replication by ATM mutation
Ataxia-telangiectasia (A-T) is an autosomal recessive multisystem disorder characterized by ocular and cutaneous telangiectasia, hyper sensitivity to ionizing radiation, increased incidence of malignancies, and variable immunodeficiency (high risk of bacterial and viral infection). A-T results from mutations in a single gene (ataxia-telangiectasia, mutated; ATM). ATM responds to a variety of abnormal DNA structures and initiates signaling cascades leading to the DNA damage responses (DDRs). There are no reports of severe herpes simplex in A-T patients, although A-T patients show much higher rate of lethal varicella and oth...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Takenobu Yamamoto, Jeffrey I Cohen Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Cathelicidin antimicrobial peptide LL37 obligates IL-36 to induce inflammatory cytokines and chemokines from keratinocytes
Several dermatoses including psoriasis, atopic dermatitis, and rosacea alter expression of innate immune effector human cathelicidin antimicrobial peptide (CAMP). To elucidate consequence of aberrant CAMP in dermatoses, we performed the cDNA array analysis in CAMP-stimulated epidermal keratinocytes, which are primary cells in response of innate immunity and a major source of CAMP in skin. We identify 40 genes significantly induced in both undifferentiated and differentiated keratinocytes by LL37 (2.56 to 7.68μM), a representative form of human CAMP. Among LL37-inducible genes, IL-36 is of particular interest since we obse...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Na Li, Sawako Fukushi, Ryoko Shimada, Rumiko Saito, Kenshi Yamasaki, Setsuya Aiba Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Oligosaccharide modification by GnT-V promotes skin sclerosis by modulating the recruitment/activation of M2 (CD68+, CD163+) macrophages
In this study, we found that GnT-V was highly expressed in fibroblasts and infiltrating cells in the skin sections from localized scleroderma patients.Moreover, the expression of GnT-V was elevated in murine model of scleroderma induced by bleomycin (BLM) injection. To know the role of GnT-V in scleroderma, we next investigated the kinetics of BLM-induced-skin sclerosis in GnT-V-deficient (GnT-V KO) mice. GnT-V KO mice were resistant to BLM-induced skin sclerosis. Since high expression of GnT-V was observed in fibroblasts in localized scleroderma patients, we firstly assumed that functional changes of fibroblasts might be ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Arisa Kato, Mika Terao, Mizuki Yutani, Hiroyuki Murota, Eiji Miyoshi, Ichiro Katayama Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

IgE has different effects on mast cell subtypes
In this study, we investigated whether IgE could exert different actions depending on mast cell subtypes in different anatomical locations. Prior injection with hapten-specific or irrelevant IgE into the FP of wild-type or B cell knockout mice inhibited the innate-type ITH. This inhibition was detected in KitW/KitW-Vmice reconstituted with BMMCs pretreated with hapten-specific or irrelevant IgE 3-4 weeks after injection. The inhibitory effect of IgE was reproduced in the KitW/KitW-V mice reconstituted with CTLMCs without maturation period. Degranulation of WT CTLMCs induced by NMU was inhibited by pretreatment of IgE which...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yoshiko Mizukawa, Yurie Komatsu, Yoshimi Yamazaki, Tetsuo Shiohara Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Basophils promote eosinophil infiltration and activation during skin inflammation
Allergic inflammation is a result of a complex interplay among inflammatory cells, including mast cells, basophils, lymphocytes, dendritic cells, and eosinophils. These cells produce multiple inflammatory mediators. Basophils are known to infiltrate into the skin in a number of human skin diseases in coexistence with eosinophils. However the relationship between basophils and eosinophils and their roles in cutaneous immunity remain unknown.To clarify this issue, we used eosinophil-depleted ΔdblGATA-1 mice and basophil-depletable Bas TRECK transgenic mice. Firstly, we performed irritant dermatitis using topical application...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Chisa Nakashima, Atsushi Otsuka, Gyohei Egawa, Hideaki Tanizaki, Kenji Kabashima, Yoshiki Miyachi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

IL-33 triggers mast cell-mediated innate immunity against lethal herpes simplex virus infection through TNF-α and IL-6
The essential contribution of mast cells (MCs) to bacterial host defense has been well established; however, little is known about their role in viral infections in vivo. We have previously reported that MCs were critically involved in host defense at herpes simplex virus 2 (HSV-2)-infected sites through TNF-α and IL-6 production using a MC knock-in mouse model. We also indicated that MC-deficiency resulted in impaired HSV clearance at the infection sites during the first 72hrs p.i., suggesting that MCs play a key role as the first line of defense against HSV rather than contributing to acquired immunity. Interestingly, H...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Rui Aoki, Tatsuyoshi Kawamura, Fumi Goshima, Susumu Nakae, Atsuhito Nakao, Yukihiro Nishiyama, Shinji Shimada Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Stress-induced chemokine production by hair follicles recruits dendritic cells to sites of minor trauma
Mammalian hair is a vital barrier, and epidermal Langerhans cells (LC) are dendritic cells that associate with hair follicles (HF) for unknown reasons. LC arise from monocyte-derived precursors during inflammation, but detailed mechanisms by which LC are recruited to epidermis remain to be elucidated. Herein, we show that fate mapping or selective depletion of monomyeloid precursors utilizing Lysozyme M-Cre mice (crossed to CAG-CAT-eGFP or ROSA26-Diphtheria toxin A mice) reveals lysozyme M-expressing immediate LC precursors (pre-LC) that derive from Gr-1hi monocytes. Pre-LC infiltrated perturbed epidermis through HF in CCR...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Keisuke Nagao, Tetsuro Kobayashi, Kazuyo Moro, Manabu Ohyama, Kenji Kabashima, Bjoern Clausen, Young-hun Cho, Mark Udey, Masayuki Amagai Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Analysis of T cell subtypes and cytokines in NC/Nga mice sensitized by house dust mite
Atopic dermatitis (AD) is a highly pruritic, chronic relapsing inflammatory skin disease characterized by abnormal skin barrier and immune dysregulation. It has been reported that various T cell subsets are involved in this diseases; Th2 cells and secreted Th2 cytokines have great influences on the development of the AD; Th17 cells also involved in acute phase of AD; Th22 cells are responsible for chronic phase of AD; Regulatory T (Treg) cells, classified into natural Tregs (nTreg) and inducible Tregs (iTreg, such as, Tr1 cells), play a crucial role in the control of various immune cells and their responses.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Nara Lee, Shan Jin, Jin Young Kim, Yun Sun Lee, Seongmin Noh, Jungsoo Lee, Jung U Shin, Chang Ook Park, Kwang Hoon Lee Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Effect of cyclic adenosine monophosphate and prostaglandin E2 on multinucleated giant cell formation from human monocytes in sarcoidosis
Multinucleated giant cells(MGC) are characteristic cells in granulomatous disorders and derived from monocyte-macrophage lineage cells. The exact mechanisms of MGC formation are not determined. Cyclic adenosine monophosphate(cAMP) is the intracellular second messenger for immunoregulatory mediators and plays a key role in regulation of monocyte-macrophage-mediated inflammatory responses. Here we examined the effects of cAMP on MGC formation. When monocytes were cultured with ConA-stimulated mononuclear cell supernatants(CS), MGC were formed on day 3. MGC formation was significantly suppressed by adding phosphodiesterase in...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kana Mizuno, Manabu Osawa, Hirotsugu Tanimura, Fumikazu Yamazaki, Hiroyuki Okamoto Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Notch signaling augments mast cell cytokine production by direct and indirect mechanisms
In this study, we investigated the molecular mechanisms by which Notch signaling augments cytokine production by mast cells. Treatment of mouse bone marrow-derived mast cells (BMMCs) with Notch ligand led to significant increase in the production of IL-4, IL-6, and IL-13 in response to cross-linking of the high affinity IgE receptor (FcɛRI). We found that the phosphorylation of JNK and p38 MAPK induced by FcɛRI cross-linking was increased in Notch ligand-treated BMMCs compared with control cells. The augmentation of FcɛRI-mediated IL-6 and IL-13 production was also observed in BMMCs transfected with Notch2 intracellular...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Nobuhiro Nakano, Chiharu Nishiyama, Ko Okumura, Shigaku Ikeda, Hideoki Ogawa Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Signaling pathway through the inhibitory receptor E-cadherin in epidermal γδ T cells
In this study, we analyzed expression levels of known signaling molecules, which mediate inhibitory signals through other lymphocyte inhibitory receptors or through E-cadherin on epithelial cells, before and after cross-linking of E-cadherin on DETCs. Quantitative RT-PCR analysis showed no significant up-regulation of SHP-1, SHP-2, SHIP-1, BATF, or PTEN expression after the cross-linking of E-cadherin on DETCs. Previously, we have shown that E-cadherin is down-regulated upon activation of DETCs during wound healing in vivo. In the periwound activated DETCs, a transcription coactivator, Yes-associated protein (YAP) was tran...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Youhei Uchida, Kazuhiro Kawai, Atsuko Ibusuki, Takuro Kanekura Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Contribution of IL-33 to contact urticaria model
In this study, to elucidate the pathological role of IL-33 in the skin, we examined contact urticaria induced in il33+/+ or il33-/− mice.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yasutomo Imai, Takashi Haneda, Yoshiko Sakaguchi, Koubun Yasuda, Tomohiro Yoshimoto, Kenji Nakanishi, Kiyofumi Yamanishi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

SLAM-dependent and -independent mechanism of IgE induction
Accumulating evidence suggests that IgE production induced by epicutaneous sensitization with protein antigen are associated with the development of allergic diseases such as allergic asthma and food allergy as well as the pathogenesis of atopic dermatitis. However, the precise mechanism of IgE induction by epicutaneous antigen exposure remains to be elucidated.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Akihiko Kitoh, Nobuhiro Kusuba, Yoshiki Miyachi, Shimon Sakaguchi, Yusuke Yanagi, Kenji Kabashima Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Roles of regulatory T cells in atopic dermatitis-like Th2-dominant cutaneous inflammation
Atopic dermatitis (AD) is characterized by cutaneous hyper-reactivity to environmental triggers, which is attributable to barrier dysfunction and Th2 predominance. Repeated topical application of haptens to the mouse skin is known to induce AD-like Th2 dominant cutaneous inflammation. However, it remains unknown how repeated hapten challenges induce Th2-dominant inflammation, while single hapten challenge results in IFN-γ-mediated Th1-type inflammation, i.e., contact hypersensitivity.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Sho Hanakawa, Akihiko Kitoh, Yoshiki Miyachi, Kenji Kabashima Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Stimulation with anti-CD3/CD28 beads and TGF-β in the non-inflammatory milieu converts CD4+T cells into Helios+Foxp3+regulatoryT cells
CD4+Foxp3+regulatoryT cells (Tregs) control various immune responses including contact hypersensitivity and autoimmune diseases, and adoptive therapy by Tregs of such diseases has been proposed. The clinical use of Tregs will require large numbers of cells. There are two options to achieve this purpose. One is to expand Tregs. Another is to convert antigen-specific CD4+T cells into CD4+Foxp3+Tregs. In both options, it is important to obtain Tregs that possess a steadily suppressive function. Tregs are divided into two subpopulations based on the expression of transcription factor Helios. All of the thymic Tregs express Hel...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Takashi Nomura, Norihito Hayatsu, Kazunari Sugita, Kenji Kabashima, Yoshiki Miyachi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Hair development and Langerhans cell migration during human embryogenesis
Hair follicles are immune privilege sites that protect multipotent stem cells. The location of the stem cells in the bulge area not only shields these crucial cells from environmental factors, but also protects them from immune-mediated injury. Hair follicles are considered to be reservoirs of Langerhans cells (LC). Although LC are known to migrate from the bone marrow, little is known about the mechanisms by which human LC locate to their appropriate position during embryogenesis. In contrast, melanocytes originate from the neural crest and migrate from the dorsal neural tube to the ventral side during embryogenesis. Afte...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Motoki Nakamura, Saori Kasuya, Akimichi Morita Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Establishment of a method to quantify the specific IgE against sweat antigen in sera of patients with atopic dermatitis
In this study, to overcome the disadvantages of HRT, we established the enzyme-linked immunosorbent assay (ELISA) to measure specific IgE against sweat antigen by using sera of patients with AD.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Makiko Hiragun, Kaori Ishii, Takaaki Hiragun, Yuhki Yanase, Hidenori Suzuki, Shoji Mihara, Michihiro Hide Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Protective roles of STAT6 in basophil-dependent prurigo-like reactions
Prurigo is a common skin disease characterized by urticarial papules and/or nodules with severe itching. While the mechanisms underlying prurigo have yet to be determined, our previous study demonstrated that repeated administration of antigens in IgE-transgenic mice induced prurigo-like skin lesions. The present study investigated immunological events in a murine model of prurigo reactions. IgE-transgenic mice received intradermal injections of 2,4,6-trinitrophenyl ovalbumin (TNP-OVA) into dorsal skin on days 1, 4, and 7, resulting in the development of persistent nodular skin lesions. Histopathologically, marked hyperker...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Takashi Hashimoto, Takahiro Satoh, Hiroo Yokozeki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Generation of B7H1-expressing CD11b+Gr1+myeloid derived suppressor cells (MDSCs) during immune-modulatory therapy for B16F10 melanoma
Myeloid derived suppressor cells (MDSC) comprise a heterogeneous population of cells (CD11b+, Gr-1+) that can be found in tumor-bearing hosts. We previously reported that MDSC suppress the activity of T cells together with Tregs through B7H1-mediated pathways. To investigate the MDSC during melanoma treatment, we subcutaneously injected mice with 2×105 B16F10 and administered topical 5% imiquimod cream (IQM) with or without i.p. injection of Dacarbazine (Dac). FACS analysis revealed that B7H1 was significantly augmented both on splenic (s-)MDSC and tumor-derived (t-)MDSC from IQM-treated mice. In contrast, the expression ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Taku Fujimura, Yumi Kambayashi, Setsuya Aiba Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Role of IL-17A for induction of Th2 in a murine model of atopic dermatitis
Atopic dermatitis (AD) is generally regarded as a T helper 2 (Th2)-mediated inflammatory skin disease. On the other hand, it has been reported that the number of IL-17 positive cells is increased in the peripheral blood and the acute skin lesion of AD patients. However, the role of IL-17A in the development of AD remains unclear.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Saeko Nakajima, Yoshiki Miyachi, Kenji Kabashima Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Intercellular gap of keratinocytes is a migratory pathway for epidermal Langerhans cells
The epidermis is composed of keratinocytes that bind tightly each other. This feature contrasts to the property of the dermis where cellular density is low and filled with extracellular matrix. Therefore, it remains a question how Langerhans cells, a subtype of dendritic cells in the epidermis, migrate within such a “packed” area. Here, we examined the motility of Langerhans cells in the contact hypersensitivity response by means of two-photon microscopy. First, we labeled cellular membrane of keratinocytes with a fluorescent lectin, and revealed that there exist 1-2nm gaps between adjacent keratinocytes in vivo. In th...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Gyohei Egawa, Yohei Natsuaki, Yoshiki Miyachi, Kenji Kabashima Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

ASK1 plays a critical role in the elicitation phase of contact hypersensitivity response through promotion of IL-17 production
Contact hypersensitivity (CHS) is a form of delayed-type hypersensitivity, an immune response to a variety of contact sensitizers. However, The precise mechanism of the elicitation phase of CHS remains to be elucidated.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Junya Mizukami, Kohsuke Takeda, Hidenori Ichijo, Ryoji Tsuboi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Dermal dendritic cells initiate the elicitation phase of contact hypersensitivity via immunological synapse formation
Contact hypersensitivity (CHS) is a murine contact dermatitis model that consists of the sensitization and elicitation phases. Although the roles of cutaneous dendritic cells (DCs) in the sensitization phase have been extensively studied, their roles in the elicitation phase remain largely unknown. Initially, we evaluated the antigen presenting capacity of cutaneous DCs by DC subset-specific depletion system using CD11c-DTR (diphtheria toxin receptor) and Langerin-DTR mice in combination with bone marrow chimeric techniques. Depletion of LCs alone in the elicitation phase did not change CHS responses 24h after elicitation,...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yohei Natsuaki, Gyohei Egawa, Satoshi Nakamizo, Hideaki Tanizaki, Takashi Hashimoto, Yoshiki Miyachi, Kenji Kabashima Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

A20 tumor regressions by rR9-GRIM19 is elicited via both CD8+and CD4+T cell conversion of IL-10 into IL-17/IFNgamma-producing phenotypes
To inhibit the STAT3-mediated signaling cascades in cancer microenvironment, we generated a fusion protein, designated as rR9-GRIM19. Our previous results demonstrated that intratumoral (i.t.) injections of rR9-GRIM19 alone elicited complete regression of established A20 tumor (B-cell lymphoma line with phosphorylated-STAT3; pSTAT3 expression). Our results also demonstrated that rR9-GRIM19-mediated antitumor effects were not observed in A20-bearing nude mice, but those were restored simply by the adoptive transfer of normal CD8+T cells. Based on these results, we investigated how T cells participated in antitumor effects. ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Fumie Hanawa, Naotaka Shibagaki, Shinji Shimada Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Regulation of TCR-induced stop signal and T cell-effector function in peripheral tissue through PD-L1-PD-1 signaling
T Cell-mediated adaptive immunity is critical for host defense, but little is known about T cell behavior and the regulatory mechanisms during delivery of effector function in peripheral tissue. To address this issue, we utilized two-photon intravital microscopy and flow-cytometry to explore the dynamic process of effector T cells activation through T cell receptor (TCR)-signaling. Using Delayed Type Hypersensitivity as a model, we first investigated the relationships among antigen presentation, T cell motility, local production of the effector cytokine by CD4+T cells in skin. We found that whereas effector T cells exhibit...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Tetsuya Honda, Jackson_G Egen, Ronald_N Germain Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

CD155 is highly expressed by melanoma tissues and it suppresses the activation of melanoma specific CTLs via interaction with TIGIT
In this report we evaluated impact of CD155 expressed by melanoma cells on melanoma reactive CTLs. First, we found that CD155 was highly expressed by all melanoma cell lines (6/6) and tissues (5/5) we examined. Then, we checked direct effect of CD155 expressed by melanoma cells on activation of CTLs in vitro. Activation of melanoma reactive CTLs assessed by cytokine release was significantly suppressed when co-cultured with melanoma cells retrovirally transduced with CD155 compared to mock transfectants, and the suppressive effect was comparable to that of PD-L1. The experiment using the truncation mutant of CD155 suggeste...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Takashi Inozume, Junpei Furuta, Kazutoshi Harada, Shinji Shimada Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The biologic effect of high power femtosecond-terahertz pulses on the skin of C57BL/6 mouse
This study was to evaluate the safety and the biologic effect of THz irradiation using C57BL/6 mice model. After being clipped, the back skin of mouse was exposed femtosecond (fs)-THz beam for 1hour. The fs-Thz beam used in this study contained 10Hz pulses (mean power¦ 0.254 nJ/s) and each pulse has frequency up to 3 THz and the pulse width of 10μJ. Hematoxylin and eosin (H&E) stains of biopsied samples showed no difference between THz-irradiated and sham mice. The quantitative real time-polyermase chain reaction (PCR) analysis showed that gene transcription of substance P was significantly decreased 24hours after the ex...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Seong Jin Jo, Sun-Young Yoon, Ji Yeon Lee, Kyu-Tae Kim, Kyu Han Kim, Woong Yang Park, Oh Sang Kwon Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Nuclear localization of beta-catenin in the nail matrix cells and onychomatricoma
In this study we evaluated the expression pattern of beta-catenin in human nail unit and a rare nail tumor onychomatricoma.Normal human nail unit was obtained during polydactyly operations and cadavers. Immunohistochemistry for beta-catenin was applied to the sections of normal nail unit and onychomatricoma. In the nail unit, beta-catenin was expressed in the nucleus and cytoplasm of the suprabasal nail matrix cells. Only the cell membrane was beta-catenin-positive in other epithelial cells such as nail bed, eponychium and the epidermis of the proximal nail fold. In onychomatricoma beta-catenin was expressed in the nucleus...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Dong-Youn Lee, Cho-Rok Kim, Ji-Hye Park, Jun-Mo Yang Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

A disorder of pilosebaceous unit of Kyoto Rhino Rat (krh) and its possibility of a model for acne vulgaris
Acne vulgaris is considered as a disorder of pilosebaceous unit of the skin, and the mechanisms is still obscure. Kyoto Rhino Rat (krh) was made in mutagenesis by ethylnitrosourea (ENU) which is a chemical mutagen at Institute of Laboratory Animals Graduate School of Medicine Kyoto University. Krh is an excellent candidate of a new model for acne vulgaris with abnormality of hair follicle and sebaceous glands.Krh were used in this study at 12 weeks of age (n=12). Comedone were mildly seen at 12 weeks of age of krh. Krh were treated with adapalene and vehicle 6 times a week, for 12 weeks. Skin lesions were clinically and pa...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Takashi Yoshimasu, Takashi Kuramoto, Tadao Serikawa, Seiko Toyozawa, Nobuo Kanazawa, Yuki Yamamoto, Fukumi Furukawa Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Estrogen induce VEGF expression in dermal papilla cells
It is required to reveal the mechanisms of female pattern hair loss (FPHL) augmenting in Japanese women with age. Estrogen could have a pivotal role on the hair cycle control, because anagen extends during pregnancy. Estrogen levels in blood considerably drop in women who are in ages suffering FPHL. Therefore, we analyzed expressions of several genes associated with hair cycles using real time RT-PCR in human follicular dermal papilla cells (DPCs) after treatment with a various extent of estradiol. The cells were culture with serum-free medium without phenol red. We found that vascular endothelial growth factor (VEGF) expr...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yuuki Sakurai, Ichitaro Niibe, Satoshi Ogawa, Yasufumi Takeda, Takashi Matsuzaki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

CXCL10 induces infiltrates of Th1/Tc1 cells in the acute alopecia areata followed by sustained Tc1 cell-accumulation in the chronic phase
Alopecia areata (AA) is an organ-specific autoimmune disease with cell-mediated autoimmune reactions. T lymphocytes densely surround hair bulbs in the lesion of acute AA, which is referred to as “swarm of bees”. We investigated the pathological mechanisms of “swarm of bees”, focusing on T-cell chemokine and chemokine receptors. In the PBMCs, the frequency of CXCR3+CD4+ T cells (Th1) was significantly higher in acute AA than in chronic AA or healthy control, while CXCR3+CD8+ T cells (Tc1) were significantly increased in chronic AA. In the skin lesions of acute AA, CXCR3+CD4+ and CXCR3+CD8+ T cells infiltrated in the...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Taisuke Ito, Hideo Hashizume, Takatoshi Shimauchi, Atsuko Funakoshi, Natsuho Ito, Masahiro Takigawa, Yoshiki Tokura Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Transcription factors Runx1 and Runx3 regulate differentiation and proliferation of keratinocyte
Runt-related transcription factor (Runx) family is implicated in stem cell regulation, tissue development, and oncogenesis. Runx1 and Runx3 play important roles in several organs, including blood, muscle, gastric mucosa, the nervous system, and hair follicles by affecting cell survival, proliferation, and differentiation. However, it is unknown whether and how Runx1 and Runx3 regulate in the epidermis. We investigated the functions of Runx1 and Runx3 in the epidermis. First, we overexpressed Runx1 and Runx3 in primary mouse keratinocyte by using adenovirus vectors. The amounts of keratin1 (K1) and keratin10 (K10) were decr...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Ryuhei Uchiyama, Eisaku Ogawa, Yukiko Kiniwa, Hisashi Uhara, Won F. Wong, Kazuyoshi Kofu, Shuntaro Ikawa, Masanobu Satake, Ryuhei Okuyama Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Epidermal Wnt/beta-catenin signalling promotes dermal adipocyte differentiation during hair follicle morphogenesis and regeneration
Reciprocal interactions between different lineages of cells within mammalian skin are essential for skin morphogenesis, maintenance and function. Although it has long been known that morphogenesis and cyclic expansion and regression of subcutaneous/dermal adipocytes occur in parallel with the hair regeneration cycle, the mechanisms involved in this correlation remain largely unknown. Here we show that the activation of epidermal Wnt/beta-catenin signalling in growing hair follicles not only controls hair follicle stem cell (HFSC) activation but also regulates dermal lineages to promote adipocyte differentiation. Inhibition...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hironobu Fujiwara, Giacomo Donati, Valentina Proserpio, Ken Natsuga, Charlotte Collins, Fiona Watt Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Indispensable roles of BNIP3, an inducer of autophagy, in both differentiation and maintenance of epidermal keratinocytes
Recent studies have revealed that autophagy, a lysosomal degradation pathway, is involved in differentiation of erythrocytes, lymphocytes, and adipocytes. Keratinocyte differentiation is also going along with an activation of lysosomal enzymes and organelle clearance, expecting the contribution of autophagy in this process.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Mariko Moriyama, Junki Uda, Akifumi Matsuyama, Hiroyuki Moriyama Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Human induced pluripotent stem cell line with hair forming capacity is ectodermal/epithelial lineage-prone and in WNT-activated state
Recently, we demonstrated that human induced pluripotent stem cell-derived keratinocyte precursors (hiPS-KCPs) contributed to hair follicle (HF) regeneration. Among three hiPS cell lines tested, 201B7, a hiPS line generated with 4 Yamanaka factors, better communicated with hair inductive mesenchyme than WD39 and WDT2 (4 and 3 factor) lines and provided optimal material for HF formation experiments. The aim of the current study is to elucidate underlying mechanisms that endowed 201B7 hiPS line such functional advantage enabling HF reconstitution. To probe natural fate, embryo bodies were generated from each hiPS line and al...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Ophelia K. Veraitch, Tetsuro Kobayashi, Yoichi Imaizumi, Wado Akamatsu, Masayuki Amagai, Hideyuki Okano, Manabu Ohyama Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Telogen Phase of Hair Growth Cycle Is Regulated by Cyclic Epithelial FGF18 Signaling
Hair follicles repeatedly cycle through growth (anagen), regression (catagen), and resting (telogen) phases. Although the signaling molecules involved in the anagen and anagen-catagen transition have been studied extensively, the signaling that controls telogen is only partly understood. Here we show that fibroblast growth factor (Fgf)18 is expressed in a hair stem cell niche throughout telogen, and that it regulates the hair cycle through the non-growth phases. When the Fgf18 gene is conditionally knocked out in keratin 5-positive epithelial cells in mice, telogen becomes very short, giving rise to a strikingly rapid succ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Toru Imamura, Yuko Oda, Junko Oki, Akiko Komi-Kuramochi, Emi Honda, Masahiro Asada, Masashi Suzuki, Miho Kimura-Ueki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Increased Expression of hypoxia-glycolysis-acidosis sequence related proteins: An important role in keloid pathogenesis
In conclusion, hypoxia plays an important role in keloid pathogenesis and CA9, as an end point of hypoxia-glycolysis-acidosis sequence, may be a therapeutic target for keloid.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Xianglan Zhang, Zhenlong Zheng, Joonger Park, Hye Rang On, Kee Yang Chung, Mi Ryung Roh Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Fibroblast protective effect of Royal Jelly against deleterious lipid peroxidation by-products
Royal jelly (RJ) is known as an oral healthy tonic. It is also used for its wound healing properties. After analysis of its chemical composition, a RJ from the French Sologne area was tested on cultured human fibroblasts for its nutritive properties and protective capacities against the lipid peroxidation by-products deleterious effects. Lipid peroxidation by-products are known to impair protein detoxification, to be linked to elastin fiber fragmentation (elastosis) and facial skin color modification during ageing. RJ analysis revealed specificity in 10-HDA, protein, sugar and aminoacid levels.In order to evaluate the RJ n...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Frederic Bonte, Marcela Otranto, Aurore Danigo, Jean Christophe Archambault, Alexis Desmouliere Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

WITHDRAWN
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Dissecting two-cell colony dynamics prefigures clonal growth of HaCaT keratinocytes
Human epidermal keratinocytes have heterogeneity in their proliferative capacity and the cells with the greatest ability for proliferation in culture are stem cells. Isolation and selective cultivation of keratinocyte stem cells with a non-invasive procedure facilitate clinical applications of autologous keratinocytes. Here we demonstrate that dynamics of HaCaT keratinocytes at early phase in culture is associated with their proliferative capacity and that continuous growth of keratinocytes can be predicted by dissecting cellular dynamics in small colonies. HaCaT cells generated two-cell colonies after first mitosis and th...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Imai Matome, Sota Tate, Shigeki Higashiyama, Daisuke Nanba Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Growth factors secreted by stem cells in dermis and subcutaneous adipose tissues and their roles in skin homeostasis
In this study, focusing on growth factors secreted from stem cells, we examined the effects of these growth factors on homeostasis and regeneration of skin tissue.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yuichi Hasebe, Shiro Ohgo, Seiji Hasegawa, Hiroshi Mizutani, Satoru Nakata, Akiko Yagami, Kayoko Matsunaga, Noriko Saitoh, Kenji Matsumoto, Masashi Toyoda, Akihiro Umezawa, Hirohiko Akamatsu Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

MicroRNA Expression Profiles in Keloid and Normal Dermal Fibroblasts
Purpose: Keloids develop through abnormal wound healing process and are characterized by fibroblastic cell proliferation and excessive collagen synthesis but its etiology is still unknown. On the other hand, microRNAs are a highly conserved class of noncoding RNAs with important regulatory functions in cell proliferation, differentiation, and apoptosis. To discover novel regulatory pathways involved in keloid development, we performed microRNA expression profiling of keloid and normal dermal fibroblast cell cultures. Methods: Primary cultures of fibroblasts derived from keloid and normal dermis were used after being charac...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hajime Shimizu, Shinichi Igota, Mamiko Tosa, Seiko Egawa, Mohammad Ghazizadeh Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

A Novel Small Compound Antagonizing TGF-β/Smad Signal Stimulates Migration and Proliferation of Keratinocytes and Enhances Wound Healing
Dermal wound healing is a complex process orchestrated by a number of humoral factors and cellular components such as keratinocytes, fibroblasts and inflammatory cells. Transforming growth factor-β (TGF-β) and its intracellular mediators, Smad proteins, are well known to be the principal factors that lead to tissue fibrosis. TGF-β stimulates proliferation of fibroblasts while inhibiting that of keratinocytes. It has previously been reported that administration of HSc025, a novel small compound that antagonizes the TGF-β/Smad signal through the nuclear translocation of a transcriptional repressor YB-1, suppresses the de...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hanako Yamaoka, Hideaki Sumiyoshi, Sachie Nakao, Kaori Minakawa, Yuri Takizawa, Norihiro Ikoma, Tomotaka Mabuchi, Akira Ozawa, Kiyoshi Higashi, Koichi Saito, Yutaka Inagaki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research