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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 37.

Retinoids regulates the production of matrix metalloproteinases in the human epidermal keratinocyte cell line HaCaT
In this study, we tried to investigate the suppressive effect of adapalene, all-trans retinoic acid and 9-cis retinoic acid on the production of MMP-1, 9, and 13. Cultured HaCaT keratinocytes were stimulated with TNFα, with or without adapalene, all-trans retinoic acid or 9-cis retinoic acid, and the supernatants were collected and subjected to ELISA. RNA was also extracted, and real-time PCR was performed. HaCaT keratinocytes produced MMP-1,9 and 13, which were induced by TNFα. Aadapalene, all-trans retinoic acid and 9-cis retinoic acid suppressed the induction of MMP-1,9 by TNFα, in a dose-dependent manner, but not that of MMP-13.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Satomi Hosoda, Jitlada Meephansan, Masaru Karakawa, Tomoyuki Oshio, Hidetoshi Tsuda, Mayumi Komine, Mamitaro Ohtsuki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Upregulation of TGF-β1 and basic FGF develops elastofibroma
Elastofibroma is a rare tumor that occurs most typically in the lower end of the subscapular space of middle-aged to elderly persons. One of the speculated etiologies is repeated trauma due to mechanical friction of the scapula against the ribs, and the other to be suggested is a nontraumatic, genetic origin. However, exact etiology of elastofibroma is still unknown. Recently, it has been reported immunohistologically that fibroblasts in elastofibroma may produce both abnormal elastic fibers and collagen fibers through the action of TGF-β which promotes fibroblast proliferation. We investigated the expression of TGF-β1an...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hisayoshi Imanishi, Daisuke Tsuruta, Yasuhiko Yoshida, Aya Okabayashi, Chiharu Tateishi, Hirofumi Ikushima, Ren Nagasako, Masamitsu Ishii, Koichi Nakagawa Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The glucose availability and the induction of HIF-1 alpha expression determine the imiquimod induced apoptosis in cancer cells
In this study, we observed that imiquimod promoted extracellular glucose depletion, enhanced lactate production and decreased oxygen consumption in several tumor cell lines. Imiquimod not only activated PI3K/Akt signaling but also increased HIF-1 alpha expression which is the key regulator of aerobic glycolysis. The imiquimod induced HIF-1 alpha over-expression may partially regulated by translational level. The pharmacological inhibition of PI3K/Akt signaling and knockdown HIF-1 alpha expression in imiquimod-treated cells could significantly reduce the rate of extracellular glucose depletion and enhance cell apoptosis. We...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Jeng-Jer Shieh, Shi-Wei Huang Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Synergistic effects of sphingosine kinase 1 inhibitors and anti-neoplastic agents on melanoma cell apoptosis
In this study, we investigated the effect of cisplatin, a DNA damaging agent, and its combined effect with FTY720, S1P receptor antagonist, or SK inhibitor (SKI) on the production and function of SPHK1 in cultured melanoma cells. Western blot analysis showed that cisplatin caused a reduction in SPHK1 at protein level. This down-regulation was post-transcriptional, because the mRNA level of SPHK1 by real time PCR was not suppressed. As a result, apoptosis was accelerated, as indicated by caspase 3/7 fluorophotometry. Furthermore, the combination of FTY720 or SKI with cisplatin caused additional increase in apoptosis. A decr...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Masaru Naito, Akihiko Kato, Taichi Miyazaki, Asako Ishitsuka, Etsuko Fujine, Yoshiko Banno, Mariko Seishima Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Diagnostic meaning of immunohistochemistry-based Cell Cycle Detection on human cutaneous tumors derived from keratinocyte
We often encounter the difficulties in the differential diagnosis of keratinocyte-related tumors such as seborrheic keratosis (SK), keratoacanthoma (KA), or squamous cell carcinoma (SCC). Human cutaneous tumors derived from keratinocyte are supposed to occur with dysregulation of cell cycle. P53 gene is a key regulator of cell cycle, and is known as a tumor suppressor gene. The mutation of p53 gene is detected in majority of malignant cutaneous tumors derived from keratinocyte such as SCC and Bowen's disease (BD), and is widely used as a marker for malignancy, meaning uncontrolled cell proliferation. However, it is also fo...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Toshinori Bito, Emmy Yanagita, Ryosuke Matsuoka, Tomoo Itoh, Chikako Nishigori Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Arsenic treatment promotes centrosome amplification through transcriptional regulation of Aurora-A by E2F1 in keratinocyte cell
Arsenic-induced chromosome aberration is an early event of carcinogenesis. However, the cytogenetic mechanism is still unclear. Aurora-A, a member of the Aurora mitotic kinase family, regulates centrosome duplication and chromosome stability during mitosis. Here, Aurora-A mRNA expression was increased in arsenic-related cutaneous SCC, and protein expression in the tumor lesions of arsenic-associated Bowen's disease and SCC was elevated and correlated with the progression of tumor malignancy. Furthermore, under low dose of arsenic treatment, Aurora-A in the immortalized keratinocyte was transcriptionally raised by E2F1, whi...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Chin Han Wu, Ya-Shih Tseng, Hsiao-Sheng Liu, Hamm-Ming Sheu Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

LC3 and LAMP2 is co-localized, and autophagy is involved in cell survival of angiosarcoma.
Angiosarcomas(AS) are rare soft-tissue sarcomas of endothelial cell origin that have a poor prognosis. Several groups reported the use of sorafenib, a broad-spectrum tyrosine-kinase inhibitor targeting VEGF receptors, but its efficacy is limited. Autophagy is a homeostatic “self-eating” process which involves the digestion of cytoplasmic components via the lysosomal pathway, and this process render cells nutrient for stress or starvation including lack of growth factor receptor signaling through mTOR. To improve treatments for AS, we examined if the autophagy system is involved in AS cell survival. First we examined th...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Toshiya Takahashi, Kenshi Yamasaki, Natsue Sawaya, Setsuya Aiba Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

A rapid and efficient newly established method to detect COL1A1-PDGFB gene fusion in dermatofibrosarcoma protuberans
Background: The detection of fusion transcripts of the collagen type Iα1 (COL1A1) and platelet-derived growth factor-BB (PDGFB) genes by genetic analysis has recognized as a reliable and valuable molecular tool for the diagnosis of dermatofibrosarcoma protuberans (DFSP). To detect the COL1A1-PDGFB fusion, all previous reports performed reverse transcription polymerase chain reaction (RT-PCR) using 16 forward primers from COL1A1 and a specific reverse primer from PDGFB exon 2. However, it has possible technical difficulties with respect to the handling of multiple primers and reagents in the procedure.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yoko Yokoyama, Akira Shimizu, Etsuko Okada, Osamu Ishikawa, Sei-ichiro Motegi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Surface plasmon resonance biosensor detects the altered patterns of signals in the fibroblasts expressing oncogenic HRAS
In this study, we hypothesized that SPR biosensor could distinguish between non-tumorigenic cells and tumorigenic cells by the pattern of AR change. To test this hypothesis, we genetically introduced oncogenic human HRAS (HRAS-G12V) into the non-transformed fibroblasts and studied the effect of the oncogenic transformation on the pattern of SPR signal induced by EGF. The mock-transfected MEF/3T3 tet-off fibroblasts, which are reported as non-tumorigenic, showed a full triphasic change of AR in response to EGF. In contrast, HRAS-G12V-transformed fibroblasts showed a monophasic change of AR in response to EGF. These results ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Takaaki Hiragun, Tetsuji Yanase, Tomoko Kawaguchi, Makiko Hiragun, Yuhki Yanase, Michihiro Hide Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

TGF-β1-mediated transcriptional regulation of CXCR4 in basal cell carcinoma is mediated by ERK1/2 and ETS-1 pathways
In this study, we investigated the mechanisms involved in the regulation of CXCR4 expression in invasive type BCCs. We found that invasive type BCC specimens had higher mRNA levels of transforming growth factor-β1 (TGF-β1) and connective tissue growth factor (CTGF) than non-invasive type BCCs. Non-contact co-culture of dermal fibroblasts with human BCC cells also increased the expression of CXCR4 in human BCC cells. Treatment of recombinant human TGF-β1, but not CTGF, enhanced the mRNA levels of CXCR4 in time- and does-dependent manners. The protein level and surface expression of CXCR4 in human BCC cells were increased...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yi-Shuan Sheen, Chia-Yu Chu, Shih-Ting Cha, Hsien-Ching Chiu, Min-Liang Kuo, Shiou-Hwa Jee Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

TOX is a specific marker for cutaneous T cell lymphomas
In this study, we evaluated TOX expression in biopsy specimens from various types of cutaneous lymphoma. The followings were enrolled; MF (2 patch, 2 plaque, and 2 tumor samples), SS (2 samples), anaplastic large cell lymphoma (ALCL; 5 samples), adult T cell lymphoma (ATL; 5 samples), diffuse large B dell lymphoma (DLBCL; 5 samples), lymphomatoid papulosis (Lyp; 5 samples), and peripheral T cell lymphoma not otherwise specified (PTL, NOS; 5 samples). We also evaluated TOX expression using skin specimen from healthy controls (5 samples) and AD (2 samples). Dermal atypical lymphocytes showed a high specific nuclear staining ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Sohshi Morimura, Makoto Sugaya, Hiraku Suga, Tomomitsu Miyagaki, Hanako Ohmatsu, Hideki Fujita, Yoshihide Asano, Yayoi Tada, Takafumi Kadono, Shinichi Sato Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The roles of Insulin-like growth factor-II messenger RNA (mRNA)-binding protein-3 (IMP-3) in melanoma progression
Insulin-like growth factor-II messenger RNA (mRNA)-binding protein-3 (IMP-3) is a member of insulin-like growth factor-II mRNA-binding protein family and is expressed during embryogenesis and in some malignancies. Recently, IMP-3 has been found to be a novel progression marker in malignant melanoma.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Chia-Yu Chu, Yi-Shuan Sheen, Kuanyin K. Lin, Meng-Chen Hsieh, Hsien-Ching Chiu, Min-Liang Kuo, Shiou-Hwa Jee Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Regulation of IL-33 expression by inflammatory cytokines and its putative role in inflammatory skin disorders
Interleukin (IL)-33, a member of the IL-1 family, has been implicated in Th2 immune reactions. We investigated the effect of Th-1, Th-2 and Th-17 cytokines on IL-33 expression in normal human epidermal keratinocytes (NHEKs).IFN-γ, IL-4, and IL-17A dose- and time-dependently induced IL-33 expression; this was dependent on extracellular signal-regulated kinase (ERK), p38, and epidermal growth factor receptor (EGFR) phosphorylation. We found that the inhibition of signal transducer and activator of transcription (STAT) 3, but not that of STAT1, suppressed the induction of IL-33 expression by IFN-γor IL-17A. TNF-α had no ef...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Jitlada Meephansan, Mayumi Komine, Hidetoshi Tsuda, Tomoyuki Oshio, Masaru Karakawa, Shin-ichi Tominaga, Mamitaro Ohtsuki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

βKlotho regulates the differentiation and tumorgenesis of epidermal keratinocytes
klotho gene is a putative aging suppressor gene. Klotho protein exists in two forms: membrane Klotho and secreted Klotho. The secreted Klotho has been suggested to have anti-oxidative stress/anti-cancer properties partially by inhibiting type I insulin-like growth factor receptor (IGF-IR)/extracellular signal-regulated kinase (ERK) 1/2 activity in some human organs. βKlotho is a member of the Klotho protein family. Immunohistological analysis revealed that the expression of βKlotho protein was reduced in the lesion of human squamous cell carcinoma (SCC) and diminished in the lesion of basal cell carcinoma (BCC) compared ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kozo Nakai, Shigeaki Inoue, Reiji Haba, Yoshio Kushida, Nahomi Katsuki, Yoichiro Hosokawa, Tetsuya Moriue, Kozo Yoneda, Yasuo Kubota Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Cells with ALDH high activity in human cutaneous squamous cell carcinoma possess the capacity as cancer stem cells
The concept of cancer stem cells (CSCs) is well accepted for solid tumors that arise in wide variety of organs. Just the CSCs can initiate tumor formation by the capacity of self-renewing and differentiation. In the skin, most published reports have focused on the identification of CSCs in malignant melanoma, with less attention given to cutaneous squamous cell carcinoma (cSCC). Aldehyde dehydrogenase (ALDH) is an intracellular enzyme that is involved in converting retinol to retinoic acid. High ALDH activity has been detected in CSCs populations of some solid tumors Therefore, we wanted to determine whether high ALDH acti...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Masahito Yasuda, Jiang Chen, Steven E. Robinson, Mayumi Fujita, Dennis R. Roop Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

HSP 70kDa protein 1A inhibits histone deacetylase inhibitor-induced apoptosis through mitochondrial pathway
In conclusion, HSPA1A inhibit vorinostat-induced apoptosis through intrinsic pathway.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kazuyasu Fujii, Norihiro Suzuki, Tatsuya Kaji, Toshihisa Hamada, Masashi Idogawa, Tadashi Kondo, Keiji Iwatsuki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Cell density-dependent induction of podoplanin through EGFR-STAT3 signaling pathway contributes to increased cell invasion of SCC cells.
Background: Podoplanin (PDPN) is a glycoprotein which plays an important role on cell motility and invasion. However, the precise regulatory mechanism of PDPN-expression has not been characterized in epidermal skin tumors such as squamous cell carcinoma (SCC).
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Mizue Fujii, Masaru Honma, Hidetoshi Takahashi, Akemi I-Yamamoto, Hajime Iizuka Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Forced expression of the reprogramming factors could reverse epithelial-mesenchymal transition in human SCC cells
It was reported that induced pluripotent stem (iPS) cells from murine embryonic fibroblasts underwent mesenchymal-epithelial transition (MET). This prompted us to study whether cancer cells under epithelial-mesenchymal transition (EMT) could be reversed by introduction of the reprogramming factors. To this end, we transfected mouse-derived spindle-shaped squamous cell carcinoma (SCC) cells with the reprogramming factors (Oct3/4, Sox2, Klf4, c-Myc and Lin28) through the piggyBac transposon system and followed antibiotic selection to generate stable transformants. The reprogramming factors introduced cells demonstrated polyg...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Mikiro Takaishi, Masahito Tarutani, Junji Takeda, Shigetoshi Sano Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Artemin causes hypersensitivity to warm sensation similar to warmth-provoked pruritus in atopic dermatitis
Itch impairs quality of life in many dermatoses, especially in atopic dermatitis, and is frequently excited in warm environment. The responsible factors for explaining the mechanism of warm sensation induced itch remain poorly defined. To address this issue, we focused on the function of artemin, a member of glial cell line derived neurotrophic factor, in skin. A macro array assay revealed that artemin was expressed in substance P-treated dermal fibroblasts, and immunohistochemical staining and in situ hybridization confirmed accumulation and high expression of artemin in atopic dermatitis skin lesion, respectively. Artemi...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hiroyuki Murota, Mayuko Izumi, Mostafa IA. Abd El-latif, Megumi Nishioka, Mika Terao, Mamori Tani, Saki Matsui, Shigetoshi Sano, Ichiro Katayama Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Loss of organisation in CYLD defective tumours is associated with diminished DKK2 expression.
Patients carrying heterozygous germline truncating mutations in the CYLD gene develop multiple primary hair follicle related tumours. A highly organised tumour, termed cylindroma, and a highly disorganised tumour, termed spiradenoma, may both develop in the same patient. Furthermore, histological features of both tumour types have been described within the same tumour specimen. We used three-dimensional computer-aided reconstruction of these tumours to explore growth patterns of cylindromas and spiradenomas. We demonstrate contiguous growth of tumour cells in cylindromas as well as transition of cylindroma into spiradenoma...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Neil Rajan, John Burn, James Langtry, Maya Sieber-Blum, Chris Lord, Alan Ashworth Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Analysis of CXCR1 and CXCR2 single nucleotide polymorphisms in patients with Behcet diseases
In this study, we examined CXCR1 (+2607) and CXCR2 (+785,+1208) SNP frequencies between healthy controls and BD patients. No significant differences of CXCR1 (+2607) and CXCR2 (+785,+1208) allele SNP frequencies were identified between controls and BD populations; (CXCR1 (+2507) SNP: p=0.82) (CXCR2 (+785) SNP: p=0.24), CXCR2 (+1208) SNP: p=1.0). These results indicate that the association of CXCR SNP is little in BD. Further analysis will be required for the role of CXCL and CXCR in the pathogenesis of BD.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kyohei Miyano, Atsuko Neuchi, Koichiro Nakamura, Tetsuya Tsuchida Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Clinicopathological analysis of mechanic's hand associated with dermatomyositis.
“Mechanic's hand” is occasionally seen in the fingers of patients with dermatomyositis. This eruption is frequently associated with anti–aminoacyl tRNA synthetase (ARS) antibodies syndrome and interstitial lung fibrosis. We analyzed the clinical and histological characteristics of patients with mechanic's hand who had visited our hospital these 5 years. Seven cases were biopsy–proven. As a specific eruption of dermatomyositis other than mechanic's hand, Gottron's sign (6/7), periungual erythema (4/7), and heliotrope rash (2/7) were observed. Histological findings revealed hyperkeratosis, parakeratosis, and colloid ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Tatsuhiko Mori, Tomoko Hiraiwa, Takenobu Ohashi, Mikio Otsuka, Toshiyuki Yamamoto Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Lupus erythematosus profundus: Clinical and pathological features of 23 cases
In conclusion, the prognosis of LE could be predicted from the histopathological features.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Azusa Miyashita, Satoshi Fukushima, Takamitsu Makino, Yuichiro Yoshino, Junji Yamashita, Noritoshi Honda, Jun Aoi, Masatoshi Jinnin, Yuji Inoue, Hironobu Ihn Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Effects of benzo(a)pyrene on gene expression in three-dimensionally cultured human keratinocytes
The immune system is characterized by the ability to recognize and react specifically to foreign macromolecular material. The effects of many chemical compounds to immune system have been investigated, and some of them have been reported to have immunological effects. Benzo(a)pyrene is a polycyclic aromatic hydrocarbon (PAH) found in coal tar, the metabolites of which are mutagenic and highly carcinogenic. Benzo(a)pyrene has been reported to be a direct skin irritant and was also shown to be a skin sensitizer, i.e., it causes an allergic skin response in animals and humans. However, the effect of benzo(a)pyrene on inflamma...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Ami Oizumi, Chihiro Iwahara, Yasushi Suga, Hideoki Ogawa, Kenji Takamori, Kazuhisa Iwabuchi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Thymic stromal lymphopoietin expression is increased in the horny layer of patients with atopic dermatitis
Thymic stromal lymphopoietin (TSLP) is known for its capacity to induce CD11c+myeloid dendritic cells to promote Th2-skewed inflammatory responses. Although increased expression of TSLP was reported in the lesional skin of limited numbers of patients with atopic dermatitis (AD), the relationships between the degree of TSLP expression in the skin and the severity of AD, epidermal barrier function, and eruption type remain to be elucidated.To examine the relationships between the degree of TSLP expression in the skin and the severity of AD, eruption type, and epidermal barrier function using a non-invasive method in a sizeab...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yohei Sano, Koji Masuda, Risa Tamagawa-Mineoka, Hiroshi Matsunaka, Yumi Murakami, Ryoko Yamashita, Eishin Morita, Norito Katoh Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Assessment of an imiquimod-induced psoriatic mouse model in relation to oxidative stress
In conclusion, the IQM-induced mouse model showed an aberrant antioxidant system. Levels of MPO and oxidative stress were increased, and the level and activity of SOD were decreased. Since this model seemed to be an appropriate model for psoriasis, it can be used to further study the pathogenic role of redox imbalance in psoriasis.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Jin-Ok Baek, Dashlkhumbe Byamba, Tae-Gyun Kim, Dae Suk Kim, Do-Young Kim, Soo Min Kim, Min-Geol Lee Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Kurarinone, isolated from the roots of Sophora flavescens Aiton, regulates skin inflammation by regulation of the immune responses
This study provides new evidences that kurarinone may ameliorate chronic skin inflammation diseases through the suppression of pathogenic CD4+ T cell differentiation and the overall immune response.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Byung-Hak Kim, Tae-Yoon Kim Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Identification of nitrotryptophan-containing proteins in the lesional skin of atopic NC/Nga mice
Background: Nitration of tryptophan residues is a novel posttranslational modification. This modification may be involved in many pathological processes based on regulation of protein functions and activities.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Mitsutoshi Tominaga, Hiroaki Kawasaki, Ayako Shigenaga, Atsuko Kamo, Yayoi Kamata, Fumiyuki Yamakura, Kenji Takamori Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

UFM24, a novel natural product, inhibits superoxide generation and CD11b expression in activated human neutrophils
In this study, the anti-inflammatory effects of UFM24, a natural product, on activation of neutrophils were evaluated. Our data showed that UFM24 distinctly and concentration-dependently inhibited superoxide anion (O2−) generation and CD11b expression in human neutrophils induced by N-formyl-methionyl-leucyl-phenylalanine (FMLP). However, UFM24 failed to inhibit elastase release in FMLP-activated human neutrophils. Furthermore, UFM24 did not have antioxidant and superoxide-scavenging abilities. Significantly, UFM24 induced an increase in cAMP formation and protein kinase A (PKA) activity, which occurred not through an in...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Tsong-Long Hwang, Fang-Rong Chang, Yang-Chang Wu Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Decreased adiponectin levels in the patients with diffuse cutaneous systemic sclerosis
In this study, we determined the adiponectin expression in the sera and lesional skin of patients with systemic sclerosis (SSc). Serum adiponectin concentrations were measured in 32 patients with SSc, 10 patients with SLE, 12 patients with dermatomyositis and 13 normal subjects with specific enzyme-linked immunosorbent assays. Adiponectin mRNA was determined with real-time polymerase chain reaction in the skin tissues of 5 patients with diffuse cutaneous SSc (dcSSc), 7 patients with limited cutaneous SSc (lcSSc) and 7 normal subjects.There was significant reduction in serum adiponectin levels in patients with dcSSc compare...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Satoshi Fukushima, Masatoshi Jinnin, Hiroki Arakawa, Takamitsu Makino, Ikko Kajihara, Katsunari Makino, Noritoshi Honda, Yuji Inoue, Hironobu Ihn Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Pruritus in atopic dermatitis is positively correlated with serum TARC levels and negatively correlated with serum I-TAC levels
In this study, we collected serum samples twice with an eight-week interval from 10 Japanese patients with well- or moderately controlled AD. At the same time, the extent of pruritus was evaluated using visual analogue scale (VAS). We investigated serum levels of common 12 chemokines, i.e., IL-8, MCP-1, RANTES, MIP-1α, MIP-1β, IP-10, I-TAC, MIG, eotaxin, TARC, MDC, and GROα, with enzyme-linked immunosorbent assay. There were strong correlations between serum levels of MIP-1α and MIP-1β and between those of MDC and TARC, consistent with previous reports. Strong correlations among serum levels of IP-10, I-TAC, and MIG w...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Takayuki Kimura, Makoto Sugaya, Hiraku Suga, Sohshi Morimura, Hiromichi Kai, Shinji Kagami, Koichi Yanaba, Hideki Fujita, Yoshihide Asano, Yayoi Tada, Takafumi Kadono, Shinichi Sato Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Increased serum levels of matrix metalloproteinase-3 in psoriatic diseases with inflammatory arthritides
Matrix metalloproteinase (MMP)-3 is an enzyme which plays a part in the destruction of cartilage and bone in rheumatoid arthritis (RA). Patients with RA have increased serum levels MMP-3, which are correlated with parameters of inflammation such as the erythrocyte sedimentation rate, C-reactive protein, and interleukin-6 levels. Serum levels of MMP-3 have therefore been proposed as a synovial tissue-derived marker of joint inflammation and tissue destruction. We measured serum levels of MMP-3 by enzyme-linked immunosorbent assay (ELISA) in patients with joint involvement associated with palmoplantar pustulosis and psoriasi...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Takenobu Ohashi, Toshiyuki Yamamoto Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Fine tuning by effector T cells and regulatory T cells in the imiquimod-induced psoriasis-like skin lesion
In this study, we first observed the robust accumulation of Treg as well as effector memory αβ and γδ T cells in the imiquimod-induced psoriatic skin lesion. Then we sought to reveal the roles of Treg cells in the pathogenesis of imiquimod-induced psoriasis-like skin lesion by selective depletion of Treg using Foxp3hCD2/hCD52 reporter mice, which allow selective depletion of Foxp3+Treg cells by injecting anti-hCD2 antibodies. We will exhibit the findings, which may contribute to the elucidation of fine tuning mechanism of psoriasis between effector memory T cells and Treg cells.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Nobuhiro Kusuba, Akihiko Kitoh, Yoshiki Miyachi, Kenji Kabashima Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Modulation of double-stranded RNA- and cytokine- induced responses of human keratinocytes by LL-37.
Background: Besides its microbicidal function, LL-37 can activate different types of cells. Another way, by which LL-37 modulates cellular response, is via forming complexes with the nucleic acids, such as CpG DNA and double-stranded RNA (dsRNA). LL-37 production is high in skin of psoriasis patients but not atopic dermatitis patients. A synthetic dsRNA, polyI:C, stimulates human keratinocytes to produce cytokines/chemokines including a Th2-inducing cytokine-thymic stromal lymphopoetin (TSLP) (Kinoshita et al. JACI 2009). Psoriasis is considered as a Th17-associated skin inflammation. We examine the effect of LL-37 on poly...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Xue Chen, Toshiro Takai, Yang Xie, Ko Okumura, Shigaku Ikeda, Hideoki Ogawa Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Analysis of T cells in hapten-induced contact dermatitis on TRPV1-deficient mice
Transient receptor potential vanilloid receptor 1 (TRPV1) is a nonselective cation channel activated by capsaicin, heat and acid, and thus is considered to alert noxious signals to the body mainly with pain and neurogenic inflammation. To evaluate a role of TRPV1 in the antigen-specific allergic reaction with skin inflammation, the hapten-induced contact dermatitis model was adopted for TRPV1-deficient mice.As reported at the last JSID meeting, stronger ear swelling was observed in TRPV-deficient mice than in control C57BL/6 mice at each challenge by both DNCB and PiCl applications. Regarding the adoptive transfer experime...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hong-jin Li, Fukumi Furukawa, Nobuo Kanazawa Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Intravenous immunoglobulin treatment recovers the down-regulated levels of Th1 cytokines in the sera and skin of scleroderma patients
Systemic sclerosis (SSc) is an autoimmune disease characterized by fibrosis of the skin and internal organs. There is an urgent need to develop new therapeutic approaches against skin fibrosis. Although intravenous immunoglobulin (IVIG) may be one of the promising treatments, the mechanisms by which IVIG improves the fibrosis of SSc remain unknown. To compare the cytokine profile in the sera and skin of SSc patients before and after IVIG administration, and try to clarify the mechanism of the effect of IVIG. Cytokine array revealed that the serum levels of IFN-γ and IL-12, representative Th1 cytokines, were increased by I...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hideo Kudo, Masatoshi Jinnin, Keitaro Yamane, Takamitsu Makino, Ikko Kajihara, Katsunari Makino, Noritoshi Honda, Wakana Nakayama, Satoshi Fukushima, Hironobu Ihn Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Thioredoxin Suppresses Irritant Dermatitis to Croton Oil via the Inhibition of Cytokines and Chemokines Production by Keratincytes
Thioredoxin(TRX) is a 12-kDa protein with redox-active dithiol in the active site -Cys-Gly-Pro-Cys- and has excellent anti-inflammatory effect in treating animal models of various inflammatory diseases. We have previously demonstrated that the irritant dermatitis to croton oil was attenuated in human TRX-overexpressing mice, but it remains unclear whether administration of recombinant human TRX protein has therapeutic effects on the irritant dermatitis. Here we showed that swelling and the infiltration of neutrophils were strongly inhibited by application of rhTRX in irritant dermatitis to croton oil. rhTRX distributed onl...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hai Tian, Atsushi Fukunaga, Kumiko Taguchi, Susumu Fujiwara, Hiroshi Nagai, Yoshiyuki Matsuo, Junji Yodoi, Chikako Nishigori Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

IL-17 and IL-22 induced IL-1B, IL-8, TNF-A secretion via inhibitor of nuclear factor KB kinase-A (IKKA) expression in HaCaT cell line
In this study, we demonstrated that IL-17 and IL-22 induced IL-1B, IL-8, TNF-A secretion via IKKA expression; this suggests that through the induction of IKKA, IL-17 and IL-22 affected pathogenesis of inflammatory skin disease, e.g., psoriasis, by inducing inflammation via secretion of pro-inflammatory cytokines.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kyung Ho Lee, Kyung-Ah Cho, Jin-Young Kim, So-Youn Woo Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Novel anti-acne action of β-cryptoxanthin that inhibits sebum production and lipid-droplet formation in hamster sebocytes
Acne vulgaris is characterized by excess sebum production, and apart from retinoids, there are few effective agents for acne therapy that directly suppresses sebaceous lipogenesis. On the other hand, β-cryptoxanthin (β-CRX), which is a carotenoid pigment most abundant in Citrus unshiu Marcovich (mandarin-orange), has been reported to exhibit anti-oxidative actions in various cell species. Although some carotenoids have been reported to exhibit beneficial actions for the improvement and maintenance of cutaneous functions, whether β-CRX regulates sebum production in sebaceous glands is not fully understood. In the present...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Takashi Sato, Maki Kuwata, Noriko Akimoto, Kimiko Kitamura, Yoshiyuki Shirakura, Katsuyuki Mukai, Akira Ito Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Identification and elucidation of miRNA's role in atopic dermatitis
Introduction: MicroRNAs (miRNAs), short noncoding RNAs known to be involved in post-transcriptional gene regulation, have recently been implicated in the pathogenesis of inflammatory skin conditions such as atopic dermatitis (AD). Our study aims to identify the miRNAs involved in the pathogenesis of AD.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Zacharias A.D. Pramono, Xuejun Mo, Emily Y. Gan, Ellen B. Lane, John E. Common, Gopinath Sundaram, Prabha Sampath, Mark B.Y. Tang Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Involvement of Th17/IL-17 in the pathogenesis of keloid
We have reported that IL-6 mediated inflammation is a key player and may be considered as a common causative factor for development of keloid. IL-17, a recently discovered pro-inflammatory cytokine, is secreted by a distinct subtype of activated CD4 T-cells known as Th17. The purpose of this study was to evaluate the role of Th17/IL-17 in the keloid pathogenesis. We first examined the distribution of Th17 cells in peripheral blood and keloid tissues obtained from the patients. There was an increased in Th17 cells as compared with normal individuals. The expression of IL-17 gene in keloid derived fibroblasts (KF) was higher...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Mamiko Tosa, Mohammad Ghazizadeh, Masahiro Murakami Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Molecular studies of six pemphigus vulgaris cases in remission stage showed paradoxical changes of high titer anti-desmoglein 3 antibodies
Although enzyme-linked immunosorbent assay (ELISA) indices of anti-desmoglein 3 (Dsg3) antibodies in pemphigus vulgaris (PV) is considered to reflect disease activity, we experienced 6 PV cases with high reactivity with Dsg3 in remission stage. To investigate chronological changes during 4-15 years, 5-8 sera taken at different times with 0.5 to 3.5 year intervals from disease onset were selected in each case. All 6 cases showed relatively high Dsg3 indices with more than 400 in most sera. Indices overall declined in 3 cases, while indices in remission were higher than active stage in 3 cases. On hypothesis that this dispar...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Chika Ohata, Hiroshi Koga, Bungo Ohyama, Norito Ishii, Takahiro Hamada, Minao Furumura, Takashi Hashimoto Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Regulatory mechanisms for semaphorin 3A in human epidermal keratinocytes.
Background and Objective An increase in epidermal nerve density is considered one of the major causes of antihistamine-resistant pruritus, suggesting that the lesional skin is susceptible to stimulation and sensitive to itching. Previously, we reported a potential contribution of semaphorin 3A (Sema3A) to modulation of epidermal innervation in atopic dermatitis and dry skin. The present study was performed to analyze the regulatory mechanisms of Sema3A in normal human epidermal keratinocytes (NHEK).
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yayoi Kamata, Mitsutoshi Tominaga, Atsuko Kamo, Suhandy Tengara, Kenji Takamori Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Tug-of-war between IFN-γ-producing CD8+T cells and IL-4-producing CD4+T cells destines the severity of muscle injury in dermatomyositis
Conclusions. Our observations suggest that a tug of war between IFN-γ-producing CD8+T-lymphocytes that augment inflammation and IL-4-producing CD4+T-lymphocytes that abrogate inflammation destines grade of muscle injury of DM.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Toshiharu Fujiyama, Taisuke Ito, Yoshiki Tokura, Hideo Hashizume Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Superoxide dismutase 3 controls adaptive immune responses and contributes to the inhibition of OVA-induced allergic airway inflammation
The superoxide dismutase 3 (SOD3) is an isoform of SOD. Extensive studies have been focused on role of SOD3 as an antioxidant. However, the role of SOD3 in the immune responses that contribute to the inhibition of allergic lung inflammation has not been investigated. Here, we report for the first time that SOD3 specifically inhibits dendritic cell maturation. Subsequently, SOD3 controls T cell activation and proliferation, and Th2 and Th17 cell differentiation. As a consequence, the administration of SOD3 into mice alleviated Th2 cell- mediated OVA-induced allergic asthma. In addition, we demonstrated that SOD3 inhibits OV...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Myung-Ja Kwon, Yoon-Jae Jeon, Tae-Yoon Kim Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Activation of protease-activated receptor (PAR)-2 by pemphigus vulgaris IgG in cultured human keratinocytes
Pemphigus vulgaris (PV) is an autoimmune disease in which autoantibodies cause acantholysis. Although proteases such as plasmin and matrix metalloproteinase were shown to be involved in acantholysis, the role of protease-activated receptor-2 (PAR-2) in the PV IgG-induced acantholysis remains uncertain. Therefore, we investigated whether PV IgG could activate PAR-2 on keratinocytes and induce p38MAPK activation, which is known to be involved in acantholysis, via PAR-2. Moreover, we examined PAR-2 expression in PV lesions using immunofluorescence staining. The effect of PV IgG on Ca2+signaling was measured using a fluorescen...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Song-Ee Kim, Sang Eun Lee, Soo-Chan Kim Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Circulating central memory T cells are increased and be a potential precursor of pathogenic T cells in patients with psoriasis
Central memory T cells (TCM) are similar to naive T cells and express the lymph node homing receptors L-selectin (CD62L) and CCR7. TCM have fewer effector functions, but proliferate vigorously and develop into effector T cells through antigen stimulation. Psoriasis patients have elevated levels of CD4+CCR7+ cells, but the pathogenesis of TCM in psoriasis is unclear. We therefore investigated the levels of CCR7+ T cells and CCR7+CXCR5+ T cells in the peripheral blood of psoriasis patients and the relationship between TCM and Th17/Th22 cells. CXCR5 is a more suitable marker for distinguishing between naïve T cells and TCM. ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Mai Hayashi, Takuya Furuhashi, Emi Nishida, Chiyo Saito, Kan Torii, Akimichi Morita Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Prolactin induces Th17 cytokine/chemokine production in murine imiquimod-induced psoriasiform skin
Background: A pituitary-derived hormone, prolactin (PRL) can act as a cytokine and is suggested to promote the development of psoriasis, a Th1/Th17-mediated inflammatory dermatosis. PRL can increase Th1 cytokine expression in lymphocytes but its effects on Th17 responses are unknown.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Carren Sy Hau, Naoko Kanda, Yayoi Tada, Shinichi Sato, Shinichi Watanabe Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Spontaneous Development of a Lupus-like Phenotype with ANA and anti-dsDNA Autoantibodies in the Absence of Functional Regulatory T cells
Scurfy mice lack functional regulatory T cells (Treg) due to a mutation in the foxP3 gene encoding a transcription factor crucial for the development and function of Treg. Scurfy mice spontaneously develop autoimmune inflammation in multiple organs including the skin and this disease has been shown to be CD4+T cell-mediated. It is not known, what type of autoimmune skin disease develops in Scurfy mice. To answer this question, we used different methods to analyze the skin phenotype of Scurfy mice: Histological analysis of Scurfy and WT skin reveals strong interface dermatitis with effacement of the dermo-epidermal junction...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Eva N. Hadaschik, Xiaoying Wei, Britta Heckmann, Alexander H Enk Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

T cell-derived CCL20 as a possible relevant factor in formation of dermal inflammatory clusters of chronic plaque psoriasis in situ
In this study, we explored the expression pattern of chemokine receptors on the psoriatic lesional DCs and also investigated corresponding T cells-driven chemokine which may contribute to lesional DCs/T cells interaction. Although CCR1+cells are not increased in number in psoriatic lesional skin compared to normal skin, CCR6+and CCR7+cells are increased significantly and they present clustering feature. CCR6+cells in the psoriatic skin include CD11c+myeloid DCs, T cells, and, unexpectedly, DC-LAMP+mature DCs. In the flow cytometric analysis using dermal single-cell suspensions, we confirmed high expression of CCR6 on DC-LA...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Tae-Gyun Kim, Wen Hao Wu, Dae-Suk Kim, Do-Young Kim, Hyunjoong Jee, Dashlkhumbe Byamba, Yoo-Ik Yang, James G. Krueger, Min-Geol Lee Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research