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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 40.

TGF-beta1 increases cell rigidity by enhancing expression of smooth muscle actin: Keloid fibroblasts as a model for cellular mechanics
This study asked if SMA contributes to cellular intrinsic rigidity using keloid -derived fibroblasts as a model. Using atomic force microscopy and confocal microscopy, we measured cellular rigidity and the expression of SMA in keloid fibroblasts treated with exogenous TGF-beta1. There was an increase of SMA expression in keloid tissue as well as keloid-derived fibroblasts. The cell rigidity increased by TGF-beta1 in keloid fibroblasts occurred concomitantly with increases in SMA expression. TGF-beta1 receptor 1 kinase inhibitors reduced TGF-beta1-induced cellular rigidity and SMA expression. Knocking down SMA with interfer...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Chien-Hui Hong, Chih-Hung Lee, Meng-Ru Shen Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Notch downregulation in regenerating epidermis contributes to increased IL-36 expression
Notch signaling controls a number of cellular processes including cell fate decision, proliferation, differentiation and survival/apoptosis in multiple tissues. In the epidermis, Notch1, 2 are abundantly expressed, and their function is considered to regulate skin homeostasis. Notch1 functions are clarified as a molecular switch that controls the transition of cells from undifferentiated state to differentiated one in the epidermis, and Notch2 has similar functions in keratinocytes. We found that Notch1 and Notch2 were downregulated in regenerative epidermis of wounds, where keratinocytes undergo various changes. This time...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yuko Takazawa, Yukiko Kiniwa, Ryuhei Uchiyama, Rumiko Saito, Aya Uchiyama, Naomi Kumagai, Hisashi Uhara, Eisaku Ogawa, Ryuhei Okuyama Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Wnt7a opposes Notch pathway to specify neural crest-like stem cell fate toward the melanocytic lineage in human skin
Multipotent somatic stem cells with neural-crest-like characteristics have been identified in the dermis of human skin. These neural-crest-like stem cells (NCSCs) are capable of forming spheres, express neural crest stem cell markers, display extensive self-renewal capacity, and differentiate into several neural crest derivatives, including melanocytes and neural cells. So far, little is known about the biology of NCSCs. Here, we examined the role of Notch and Wnt signaling on the self-renewal and differentiation properties of NCSCs.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Mizuho Fukunaga-Kalabis, Denitsa Hristova, Joshua_X Wang, Ling Li, Meenhard Herlyn Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Trafficking of nestin-expressing cells within the mouse whisker in 3D Gelfoam® histoculture imaged in real time with confocal microscopy
We have previously discovered that nestin-expressing multipotent hair follicle stem cells are located above the hair follicle bulge and can differentiate into neurons and other cell types in vitro (Proc. Natl. Acad. Sci. USA 100, 9958-9961, 2003; Proc. Natl. Acad. Sci. USA 102, 5530-5534, 2005). The nestin-expressing hair follicle stem cells can differentiate into Schwann cells and promote the recovery of pre-existing axons when they were transplanted to the severed sciatic nerve or spinal cord (Proc. Natl. Acad. Sci. USA 102, 17734-17738, 2005; Cell Cycle 7, 1865-1869, 2008). We have also demonstrated that the whisker hai...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Jennifer Duong, Sumiyuki Mii, Fang Liu, A.R. Moossa, Robert M. Hoffman Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Nestin-expressing stem cells form the whisker sensory nerve in 3D Gelfoam® histoculture
Whisker follicles from mice expressing nestin-driven GFP (ND-GFP) in the bulge area (BA) and their sensory nerve stump were excised and placed in 3D culture supported by Gelfoam®. When the nestin-GFP cells were imaged by confocal microscopy, they were seen trafficking to the whisker sensory nerve stump. Cord-like structures extended from the nerve stump by day 10 in 3D culture. The cord-like structures consisted of nestin-expressing spindle-shaped cells, which co-expressed βIII tubulin as well as nestin-negative round-shaped cells expressing GFAP and S100. When the sciatic or trigeminal nerve, from mice expressing red fl...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Sumiyuki Mii, Jennifer Duong, Fang Liu, Yasuyuki Amoh, Norimitsu Saito, Kensei Katsuoka, A.R. Moossa, Robert M. Hoffman Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Role of MFG-E8 in skin wound healing
The objective of this study was to ascertain the role of MFG-E8 in cutaneous wound healing. First, we examined the distribution of MFG-E8 in normal murine and human skin in vivo. In the dermis, accumulations of MFG-E8 were found around CD31+blood vessels, and MFG-E8 co-localized with PDGFRβ+ pericytes. Next, we examined the role of MFG-E8 in a cutaneous wound healing mice model. Protein and mRNA levels of MFG-E8 expression in the dermis was elevated during healing of full-thickness wounds detected by immunofluorescence staining and real-time PCR. In addition, MFG-E8 localized diffusely in granulation tissue, as well as ar...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Akihiko Uchiyama, Kazuya Yamada, Etsuko Okada, Yayoi Nagai, Sachiko Ogino, Yuko Takeuchi, Mark C. Udey, Osamu Ishikawa, Sei-ichiro Motegi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Anti-HMGB1 antibody attenuates vascular hyperpermeability and promotes wound healing during ischemia-reperfusion injury model in mouse skin
High mobility group box-1 (HMGB1), a highly conserved non-histone nuclear architectural protein, shows an inflammatory cytokine-like activity in the skin. Furthermore, HMGB1 is capable of activating macrophages in injured tissues during ischemia reperfusion (IR) injury. IR, one of the major causes of pressure ulcer, is mediated through tissue ischemia, leading to excessive inflammation and enhanced skin damage. However, it remains unclear whether HMGB1 is induced in cutaneous IR injury, and plays a role in pressure ulcer formation. Therefore, we investigated the potential role of HMGB1 in the experimental IR injury model, ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Akira Kasuya, Satoshi Hirakawa, Keyue Liu, Akihiro Kishimura, Yasutaka Anraku, Kazunori Kataoka, Mikako Ogawa, Shuji Mori, Hideo K. Takahashi, Jun-ichi Sakabe, Yasuko Tomono, Masahiro Nishibori, Yoshiki Tokura Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The cell polarity protein aPKCl couples asymmetric divisions to cell fate decisions in the epidermal lineage
Through regulation of asymmetric cell division (ACD) cell polarity proteins such as the atypical protein kinase C (aPKC) determine cell fate in lower organisms. However, their role in cell fate determination in mammals is less clear. Whereas in vitro and ex vivo studies indicate an important role for aPKCs in spindle orientation coupled to cell fate, in vivo data indicate no essential role for aPKCs in these processes. The epidermis and its appendages form a self-renewing stratifying epithelium that protects organisms from external challenges and dehydration. Different populations of stem/progenitor cells guarantee constan...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Michaela Niessen, Jeanie Scott, Susanne Vorhagen, Cedric Blanpain, Michael Leitges, Carien M. Niessen Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Multiple cutaneous venous malformations associated with cerebral cavernous malformations due to a germline mutation of the PDCD10 gene
Multiple cutaneous venous malformations are known to be associated with several syndromes such as the blue rubber bleb nevus syndrome and Maffucci syndrome. Recently, we found a 57-year-old Japanese man who had multiple cutaneous venous malformations. Physical and imaging examinations showed multiple cerebral cavernous malformations (CCMs) without obvious gastrointestinal venous malformations. CCMs are vascular malformations in the central nerve system without intervening normal parenchyma characterized by abnormally enlarged capillary cavities. Generally, these lesions are asymptomatic, and are detected accidentally in ma...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Tomoya Mizutani, Takeshi Ishigami, Kazutoshi Murao, Yoshiaki Kubo Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Novel splice variants of IL-33 expressed in normal human keratinocytes
In this study, we found 7 IL-33 splice variants including 5 newly determined variants in NHEKs. Some of these variants were cloned into mammalian expression vector fused with V5 and FLAG tags, and transfected into NHEKs. Most of them were expressed in the nucleus, however, some variants that lack specific exon were expressed in cytoplasmic fraction. When IL-33 was knocked down by siRNA, cell proliferation was down-regulated revealed by BrdU incorporation assay and cell cycle assay. IL-33 is not only a cytokine but also exerts important multiple roles in the nucleus and/or cytoplasm of keratinocytes.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hidetoshi Tsuda, Mayumi Komine, Tomoyuki Ohio, Satomi Hosoda, Shin-ichi Tominaga, Mamitaro Ohtsuki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Identification of ABCC6 gene mutations in the Japanese patients with pseudoxanthoma elasticum (PXE)
Pseudoxanthoma elasticum (PXE) is an autosomal recessive disorder characterized by the aggregation, fragmentation, and calcium deposition of elastic fibers. It primarily affects organs and tissues that are rich in elastic fibers, such as the skin and mucous, eye and blood vessels. The responsible gene for PXE is ABCC6, which encodes MRP6, a transmembrane transporter protein. Although hundreds of Caucasian PXE patients had been analyzed, there was no correlation between the mutation pattern and the clinical phenotype of PXE. We have started mutation analysis of ABCC6 gene in the Japanese PXE patients. Here, we present the r...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Akira Iwanaga, Mariko Yozaki, Yosuke Yagi, Koji Maemura, Eiko Tsuiki, Takashi Kitaoka, Miki Tanioka, Hiroshi Tamura, Yosuke Yamamoto, Tomoyasu Hattori, Zenzo Isogai, Tomoo Ogi, Koichiro Yoshiura, Atsushi Utani Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Novel ADAR1 mutations including single amino acid deletion in the deaminase domain underly dyschromatosis symmetrica hereditaria
Dyschromatosis symmetrica hereditaria (DSH) is a pigmentary genodermatosis of autosomal dominant inheritance. It is characterized by hyperpigmented and hypopigmented macules on the dorsal hands and feet. Previous work has shown that a heterozygous ADAR1 mutation of the RNA-editing enzyme of adenosine deaminase acting on the RNA 1 (ADAR1) gene causes DSH, although the pathomechanism has not been clarified yet. In the present study, we identified two novel heterozygous ADAR1 mutations, one frameshift mutation c.536insC which results in p.Leu179fsX217 and the single amino acid deletion c.2615-7delTCA which results in p.Ile873...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Michihiro Kono, Yasutomo Ito, Hideyuki Ujiie, Kenichi Morimoto, Masashi Akiyama Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Papillon-Lefèvre syndrome; five novel mutations of CTSC and functional analyses of a single nucleotide polymorphism
Papillon-Lefèvre syndrome (PLS) is a rare autosomal recessive disorder characterized by palmoplantar keratosis (PPK) and early-onset periodontitis that leads to the loss of primary and secondary teeth. PLS is caused by mutations in the gene coding for cathepsin C (CTSC). CTSC is a lysosomal cysteine proteinase that activates many serine proteinases in immune/inflammatory cells. So far, there have been more than 70 different CTSC mutations reported. Here, we performed mutational analyses of CTSC in four Japanese families with PLS and identified five novel mutations. The CTSC activities of the three probands were hardly det...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Noriko Takiyoshi, Daisuke Matsuura, Ayano Umekoji, Atsuko Kato, Megumi Furuichi, Tadamichi Shimizu, Tomohisa Fukui, Yoshihiko Mitsuhashi, Hajime Nakano, Daisuke Sawamura Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

WITHDRAWN
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

microRNA deep sequencing in Sezary Syndrome
In this study, we demonstrated that miRNA sequencing is a powerful approach for dissection miRNome in CTCLs. We firstly indicate that SS miRNome is dominated by a small fraction of abundantly expressed miRNAs. Although the mechanisms by which miR-214 may regulate target genes in SS warrants further functional analysis, our data suggests that miR-214 is a robust marker for SS diagnosis and clinical management.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yongjun Qin, Kees Tensen, Maarten Vermeer, Takatoshi Shimauchi, Yoshiki Tokura, Rein Willemze Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

The molecular genetic analysis of the expanding pachyonychia congenita case collection
We report molecular analysis of families registered during the last 2 years of this expanding case collection. Mutations were found in 61 of 67 families in PC-associated keratin genes, comprising of 35 distinct keratin mutations. Nine were previously unreported mutations, bringing the total number of different keratin mutations within our collection to >80. Of the 67 new families, 40% had mutations in KRT6A, where 2 mutations, including a nonsense mutation, were previously unreported. KRT16 mutations were found in 19% of patients, where 4 of 10 distinct mutations were unreported. KRT17 defects were found in 18% of patients...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Neil J Wilson, Edel O’Toole, Leonard M Milstone, C.David Hansen, Andrew A Shepherd, Elise Al-Asadi, Mary E Schwartz, W H Irwin McLean, Eli Sprecher, Frances JD Smith Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

A novel frameshift mutation in a V2 domain of KRT1 causes ichthyosis hystrix Curth-Macklin type and may alter keratin organization
Ichthyosis hystrix Curth-Macklin type (IHCM) is a rare autosomal dominant skin disorder clinically characterized by scaling and spiky or verrucous hyperkeratosis on the extremities and/or trunk, with varying clinical severity. Palmoplantar keratoderma is also seen in most of the cases. Histologically, a presence of perinuclear vacuolization and binucleated cells in the epidermis is characteristic, but epidermolytic degeneration is not a feature of IHCM. To date, only three mutations in the C-terminal variable tail (V2) domain of keratin 1 gene (KRT1) have been reported as a cause of IHCM. Here we present a 56-year-old Japa...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Shotaro Suzuki, Toshifumi Nomura, Hiroko Umemoto, Masashi Akiyama, Hiroshi Shimizu Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Gasc1 may affect skin carcinogenesis by regulating expression level of estrogen related gene
GASC1 encodes a histone demethylase catalyzing lysine (K) of histone, specifically K9 and K36 of histone H3. It has been reported that GASC1 is located within an amplicon at 9p23-24 frequently in esophageal cancer and overexpressed in undifferentiated ES cells and breast cancer. Since these reports suggest the involvement of GASC1 in carcinogenesis, we generated Gasc1 knockout mice to analyze the role of GASC1. It was shown that Het mice developed without any remarkable phenotype, while homozygous mice are embryonic lethal. The progenies, including 28 Het and 24 wild-type mice, were treated with the two-stage chemical carc...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yumi Hata, Kyoko fujiwara, Maki Ikeda, Motoaki Kataba, Jun Igarashi, Asako Oguni, Tadashi Terui, Johji Inazawa, Hiroki Nagase, Masayoshi Soma Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Functional studies for the TRAF6 mutation associated with hypohidrotic ectodermal dysplasia
Hypohidrotic ectodermal dysplasia (HED) is a rare condition characterized by abnormal development of hair, teeth, and sweat glands. The disease shows X-linked recessive, autosomal dominant, or autosomal recessive inheritance trait. X-linked HED is caused by mutations in ectodysplasin (EDA) gene, while autosomal forms of HED are known to be caused by mutations in either EDA receptor (EDAR) or EDAR-associated death domain (EDARADD) genes. Most recently, a de novo heterozygous mutation in TNF-associated factor 6 (TRAF6) gene has been identified in a patient with HED, while the underlying disease mechanisms remained unknown. I...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hiroki Fujikawa, Atsushi Fujimoto, Muhammad Farooq, Masaaki Ito, Yutaka Shimomura Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Identification of a mutation in GJB6 gene, encoding a gap junction protein Cx30, in a family with Clouston syndrome
In this study, we analyzed a Lebanese family with Clouston syndrome and found a recurrent heterozygous mutation c.31G>C (p.Gly11Arg) in the GJB6 gene of all the affected family members. We subsequently investigated the expression of Cx30 in postnatal human skin, as well as in developing mouse embryos. In addition, we performed in vitro studies in cultured cells in order to assess the transcriptional regulation of the GJB6 gene. The results suggested that the GJB6 could be a direct target gene of p63, of which mutations are known to result in hypotrichosis and nail dystrophy, similar to patients with Clouston syndrome. Our ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Atsushi Fujimoto, Mazen Kurban, Motonobu Nakamura, Muhammad Farooq, Hiroki Fujikawa, Abdul-Ghani Kibbi, Masaaki Ito, Yutaka Shimomura Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Identification of MCP-1 as a Key Effector of IL-31 Signaling in Familial Primary Cutaneous Amyloidosis
Primary cutaneous amyloidosis (PCA) is an itchy skin disorder that is relatively common in South America and Southeast Asia. We have recently identified missense mutations from patients with familial PCA (FPCA) in either of the two subunits (OSMRβ, IL-31RA) of the receptor for interleukin-31 (IL-31). To investigate the significance of the disease-derived mutation, we reconstituted in a human keratinocyte cell line, HaCaT, a functional IL-31 receptor by exogenously expressing IL-31RA or an FPCA-derived mutant, IL-31RA-S521F. With IL-31 stimulation, monocyte chemotactic protein-1 (MCP-1) was induced in the IL-31 receptor re...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yu-Ming Shiao, Hsiang-Ju Chung, Chih-Chiang Chen, Keng-Nan Chiang, Yun-Ting Chang, Ding-Dar Lee, Ming-Wei Lin, Shih-Feng Tsai, Isao Matsuura Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Studies of cultured Hailey-Hailey disease keratinocytes revealed pathogenic role of the mutations and novel mechanism in Ca2+ homeostasis
In this study, we cultured keratinocytes (KCs) from 3 unrelated HHD patients with different ATP2C1 mutations (p.Pro307His, c.1308+1G>A, p.Gln504X). The KCs from the case with p.Gln504X mutation showed markedly reduced expression of ATP2C1 mRNA and SPCA1 protein. On the other hand, the mRNA and protein levels in cases with p.Pro307His and c.1308+1G>A mutations did not differ from those in normal controls. The p.Pro307His mutation is located adjacent to the putative Ca2+-binding residue (p.Glu308), and may lead to incompetence of Ca2+-transport by conformational change. RT-PCR analysis for the case with c.1308+1G>A mutation ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Mitsuhiro Matsuda, Takahiro Hamada, Norito Ishii, Sachiko Sakaguchi, Yoshinaka Murai, Chika Ohata, Minao Furumura, Eiichiro Tanaka, Takashi Hashimoto Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Identification of a novel gene, UVSSA/KIAA1530, responsible for UV-sensitive syndrome
In this study, we performed whole-exome sequencing of two unrelated Japanese UVSS-A patients. Using a recessive model of inheritance, we directly identified overlapping mutations in KIAA1530, a predicted gene encoding a 709 amino acid protein of unknown function. These two patients are homozygous for a c.367A>T mutation, which led to the introduction of a premature stop codon, p.Lys123*, in the KIAA1530 protein. In other patients, we identified the same homozygous mutation and a homozygous c.87delG mutation causing a p.Ile31Phefs*9 frameshift alteration. In the fibroblasts from these patients, KIAA1530 protein was not dete...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yoshito Takahashi, Yuka Nakazawa, Atsushi Utani, Koh-ichiro Yoshiura, Tomoo Ogi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Mutations in the AAGAB gene, encoding alpha- and gamma-adaptin binding protein p34, cause punctate palmoplantar keratoderma type 1
The punctate keratodermas are clinically and genetically heterogeneous, with three dominantly inherited subtypes recognized: PPKP1 (mapped to chromosome 15), PPKP2 (not mapped); and PPKP3, (mapped to chromosome 2). Here, in one extended kindred, we confirmed mapping of PPKP1 to a 6.24 Mb locus on 15q22 containing 80 genes (maximum 2-point LOD score 8.18 with D15S983). In parallel, we performed whole exome sequencing in a single case from a second kindred, which revealed more than 28,000 sequence variants. We focused on loss-of-function variants in the exome data, of which only 3 were on chromosome 15. Only 1 heterozygous m...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Elizabeth Pohler, Toshifumi Nomura, Masashi Akiyama, Akihiro Suehiro, Hiroshi Shimizu, Ons Mamai, Mozheh Zamiri, Helen Horn, Alan D. Irvine, Jennifer Hirst, Margaret S. Robinson, John A. McGrath, Colin S. Munro, W.H. Irwin McLean Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

A study on male-female ratio of Japanese psoriatic patients
Introduction: Psoriasis is a chronic inflammatory skin disorder often associated with various systemic conditions such as metabolic syndrome. Most studies worldwide reported that psoriasis affects both sexes equally. However, the prevalence of psoriasis is twice as much in males than in females in Japan. Little is known about why psoriasis is more common in Japanese men than in women.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Reiko Naito, Shinichi Imafuku, Emi Sato, Juichiro Nakayama Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Molecular typing of strains of Fonsecaea pedrosoi isolated in Japan using polymorphism of Polyketide synthase (PKS1) gene
In this study, part of a highly variable region in PKS1 gene was used to detect molecular polymorphism of the strains of F. pedrosoi isolated in Japan. Sixty strains of F. pedrosoi isolated worldwide, including 37 isolated in Japan, were selected for this analysis. We amplified part of PKS1 gene region, approximately 450bp, using PCR method. PCR products were sequenced, and cluster analysis was performed. The proposed phylogenetic tree based on PKS1 gene closely matched that based on the ITS region of rRNA gene. To consider a molecular variation of the strains isolated in Japan, this study divided them into at least four c...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Tsuyoshi Ushigami, Kazushi Anzawa, Takashi Mochizuki Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Achillea millefolium extract: an innovative anti-aging neuro-cosmetic ingredient
Proopiomelanocortin is a precursor peptide that gives rise to several neuropeptides including adrenocorticotrophic hormone (ACTH) and beta-endorphin. POMC derived peptides have been shown to be synthesized in human epidermis where they modulate numerous skin functions. Because we previously observed that melanocortin receptor-2 and micro-opioid receptor 1, the respective receptors for ACTH and beta-endorphin decreased with aging in human epidermis, we have selected an active ingredient (INCI name: Achillea millefolium extract) able to upregulate receptor expressions. The aim of the present work was to first evaluate the ef...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: S Pain, S Nakajima, C Altobelli, A Boher, L Cittadini, M Favre-Mercuret, B Sohm, B Vogelgesang, V Andre-Frei Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Effect of Yokukansan on glutamate signaling in human keratinocytes
In this study, we investigated the effects of YKS on glutamate and histamine in NC/Nga mice and human keratinocytes to elucidate the mechanism of the ameliorating effect of YKS on AD. We found that glutamate and histamine levels were increased in the skin of NC/Nga mice that developed dermatitis spontaneously under conventional conditions. Moreover, glutamate and histamine levels were decreased in the skin of YKS-treated mice, while glutamate and histamine levels remained unchanged in the skin of fexofenadine-treated mice. When we measured glutamate and histamine levels in human keratinocytes, we found that glutamate level...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Maki Wakabayashi, Takuji Yamaguchi, Naoko Funakushi, Hajime Suto, Rie Ueki, Hiroyuki Kobayashi, Hideoki Ogawa, Shigaku Ikeda Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Hochuekkito, a kampo prescription, reduced oxidative stress indirectly in ultraviolet B-irradiated mice skin
In this study, to clarify the effect of HET on the mouse skin, we measured the H2O2 scavenging ability of HET and expression levels of some mRNAs by quantitative RT-PCR from dorsal skin samples of HR-1 hairless 6-week-old male mice, which were raised by 10% HET-mixed feed for 4 weeks.HET has a limited ability to scavenge H2O2 directly. And, expression levels of mRNAs of enzymes related to inflammation, especially that of COX2, decreased in HET-fed mice, whereas the expression levels of mRNAs of proteins involved in antioxidation, including that of catalase, did not show any change. We speculated that an anti-inflammatory c...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Shigeto Yanagihara, Hiromi Kobayashi, Hisashi Tamiya, Daisuke Tsuruta, Takeshi Nakanishi, Yuri Okano, Hitoshi Masaki, Takaaki Yamada, Yasushi Hasegawa, Masamitsu Ishii Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Novel approach for improving skin roughness mediated by keratin intermediate filaments
In this study, the relationship between KIF and skin roughness was investigated in human skin. To determine the relationship between roughness and KIF state, both roughness parameter (Ra) and observation of KIF structure by transmission electron microscopy were analyzed using human skin. The results indicated a significant correlation between them. Interestingly, both deteriorated co-localization and binding capacity of K1 and K10 were observed in the disordered KIF skin. This suggests the importance of the state of K1 and K10 in construct tight KIF. Next, to improve KIF state as well as skin roughness, screening to identi...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kazuyuki Yo, Ai Oba, Akihiro Tada Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Facial sebaceous gland size determined by three-dimensional ultrasound tomography correlates with skin elasticity
In this study, we evaluated the relation between SG morphology and the skin's physical properties using this technique to reveal the potential role of SGs.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Tomoko Sugawara, Noriaki Nakagawa, Norio Shimizu, Yoshifumi Saijo, Shingo Sakai Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Effect of hyaluronan tetrasaccharides on epidermal differentiation and skin barrier function
In this study, we aimed at investigating the influence of hyaluronan tetrasaccharides (HA4, 776.3Da) on the regulation of keratinocyte differentiation in human keratinocytes, and compared HA4 with high molecular weight hyaluronan (HA, >1,200kDa). In addition, the effects of HA4 on in vivo skin barrier function were examined. Human keratinocytes were treated at doses of 1μg/mL HA or HA4. Each differentiation marker mRNA expression and proteins level were detected by real-time PCR and western blotting. Hairless mice were topically treated with either 0.1% HA or HA4 in 70% ethanol or 70% ethanol alone after UVA irradiation (...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Madoka Kage, Yoshihiro Tokudome, Fumie Hashimoto Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Galectin-7 Interacts with HMG-CoA Synthase 1 while Inducing Its Expression
In this study, yeast two-hybrid genetic system was employed to identify interacted protein with human galetin-7. Three-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, cytoplasmic (HMGCS1) was detected first by yeast two-hybrid genetic system, followed by confirmation with β-galactosidase assay and co-immunoprecipitation. Distinct interactive site of HMGCS1 with galetin-7 was detected to reside at Phe-26 using yeast two-hybrid system and verified by His-pull down and biosensor assay. The expression of HMGCS1 was proved to be up-regulated by exogenous galectin-7 and down-regulated by galectin-7 siRNA in vitro. Moreo...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Norihiro Fujimoto, Minoru Akiyama, Shingo Tajima, Takahiro Satoh Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Bilateral systematized porokeratotic eccrine ostial and dermal duct nevus showing aberrant keratin and profilaggrin expression
Porokeratotic eccrine ostial and dermal duct nevus (PEODDN) or porokeratotic eccrine nevus is a rare nevus characterized by hyperkeratotic streaks following the lines of Blaschko. It is recently shown to be a mosaic form of keratitis-ichthyosis-deafness syndrome caused by a mutation in the GJB2 gene encoding the gap junction protein connexin-26. We studied keratins, profilaggrin and filaggrin expression in a 1-year-old Japanese boy with bilateral systematized PEODDN. Immunohistochemical stains illustrated aberrant keratin10 and keratin 1 expression as early differentiation markers, and possible unusual excess profilaggrin ...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Naoki Oiso, Ichiro Kurokawa, Akira Kawada Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Allergic march model is developed by mite application on oxazolone induced atopic dermatitis hairless mice and interrupted by acidic cream
Maintenance of acidic pH in stratum corneum(SC) is an important factor for normal skin barrier function. Long standing barrier damage followed by atopic dermatitis(AD) is the initial step of allergic march that eventually progresses to respiratory allergy. We performed experiment Whether Ox induced AD murine model develops the airway inflammation after topical application and inhalation of Dermatofagoides pteronyssinus(Dp) as a novel allergic march animal model and continuous acidic cream application interrupts allergic march. During the course of oxazolone(Ox) and Dp treatment for allergic march of hairless mice, acidic c...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Hae-Jin Lee, Na Young Yoon, Noo Ri Lee, Minyoung Jung, Dong Hye Kim, Eung Ho Choi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Control factor of cutaneous Propionibacterium acnes
We examined correlations between the number of cutaneous P. acnes and each skin factor in the cheeks of 40 healthy Japanese subjects (20 females and 20 males). The number of cutaneous P. acnes was counted by the culture method after sampling using the swab method. For skin factors, we examined the amount of sebum, composition of free fatty acids, transepidermal water loss, the amount of moisture, sodium and lactic acid, and pH on the surface skin. Moreover, the effect of each free fatty acid on the proliferation of P. acnes was also examined by an in vitro experiment. In the results, the number of cutaneous P. acnes showed...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Narifumi Akaza, Hirohiko Akamatsu, Yasuyuki Sasaki, Shiori Takeoka, China Takahashi, Yasuo Mashima, Masaaki Miyawaki, Osamu Hirose, Shunji Yamada, Satoru Nakata, Kayoko Matsunaga Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Functional identification of a vesicular ATP transporter, VNUT, mediating ATP release in human epidermal keratinocytes
The skin is one of the largest organs of the body and the first site of exposure to various harmful environmental factors such as infection and UV radiation. In response to cell damage by irritant chemicals or physiological mechanical stimulation, ATP is immediately released from epidermal keratinocytes. However, the mechanisms of its release remain largely unclear. The aim of this study is to determine mechanisms underlying release of ATP, especially focusing on VNUT, a recently identified vesicular nucleotide transporter, in normal human epidermal keratinocytes (NHEKs). When stimulated with ionomycin, NHEKs released ATP,...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kaori Inoue, Kayoko Fujishita, Keisuke Shibata, Ryohei Komatsu, Yoshio Imura, Yoshinori Moriyama, Schuichi Koizumi Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Possible roles of host defense protein S100A7/psoriasin in skin barrier functions
Although our skin is always exposed to the pathogens from the outside milieu, the number and composition of the cutaneous microflora remain constant under physiologic conditions. This is due to not only the physical barrier of the skin, but also the production of host defense peptides (HDPs). Among skin-derived HDPs, S100A7/psoriasin has been shown to be the principal HDP expressed in normal adult skin. This HDP has been also described to be a component of cornified envelope. However, its function in the skin remains not well understood. Recently, we reported that various cytokines, vitamin D3, and butyrate enhance the pro...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Fumihiro Hattori, Francois Niyonsaba, Eri Ueda, Hiroko Ushio, Shigaku Ikeda, Ko Okumura, Hideoki Ogawa, Kikuhiko Okamoto Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Altered expression of dermokine in skin disorders
To better understand the biology of dermokine-beta, a glycoprotein expressed in epithelial cells, we here determined its localization under pathological conditions and examined factors that regulate its expression. We generated an anti-human dermokine-beta/gamma monoclonal antibody cross-reacting with the mouse protein. Using this antibody, immunohistological staining and Western blotting of dermokine-beta/gamma were performed with various tissue samples. Although human dermokine-beta/gamma was expressed in almost all granular layers, upper spinous layers of the skin were also stained with anti-dermokine-beta/gamma antibod...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Minoru Hasegawa, Kiyoshi Higashi, Chikako Yokoyama, Manabu Fujimoto, Kazuhiko Takehara Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Expression pattern of cannabinoid receptor according to differentiation stage of normal human epidermal keratinocytes
In conclusion, calcium induced differentiation was associated with increased expression of CB1 receptor, even though expression of CB2 receptor was not.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Kkot Byeol Kim, Seung Hee Lee, Mi Jin Jang, Seon Ah Lee, Sung-Ku Ahn, Se kyoo Jeong, Seung-Phil Hong, Hyun Jung Kim Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Development of epidermal cannabinoid receptor modulating compounds as anti-skin disease agents
In this study, we show that NCBS modulators have agonistic or antagonistic effects on human cannabinoid receptors (CB1 and CB2). We have synthesized 30 compounds based on own lipid metabolites and screened modulators regulating the activities of CB1 or CB2 receptor in CB1-, CB2- overexpressing CHO K1 cell lines. These modulators regulated not only the cytosolic cAMP levels through activation or inhibition of cannabinoid receptors, but also the phosphorylation of signal molecules such as Akt and Erk(p42/44). Moreover, We provide evidences that NCBS007 markedly suppresses human keratinocyte HaCaT proliferation by ceramide el...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Bong W Kim, Bu M Park, Jeong E Jeon, Se K Jeong Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Ovariectomy impairs epidermal permeability barrier homeostasis in mice
Background Previous studies have demonstrated that sex hormones modulate epidermal permeability barrier homeostasis, which might be related to sensitive skin at menopause or during the menstrual cycle. However, there are no experimental data that show the effects of sex hormones derived from ovary on permeability barrier function in vivo. Ovariectomy induces sex hormone deficiency similar to that in menopause.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yue Chen, Hiroo Yokozeki, Kazumoto Katagiri Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Ceramide synthase 4 is highly expressed in involved skin sites of atopic dermatitis
Ceramide is a crucial lipid in the stratum corneum (SC) which is needed to maintain barrier function and hydration of the skin. In atopic dermatitis (AD) patients who have defective skin barrier function, ceramide levels are altered. Previously, we reported that although the amount of total ceramide was lower, the amounts of smaller ceramide species (
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Shotaro Ito, Junko Ishikawa, Ayano Naoe, Hiroshi Yoshida, Akira Hachiya, Tsutomu Fujimura, Takashi Kitahara Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

JSID Abstracts
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Effects of host defense peptide cathelicidin LL-37 on tight junction barrier
Epidermal cells bind tightly together to provide a physical barrier against injurious chemicals or pathogens from the outside milieu. One of the skin barriers is tight junctions (TJs), a structure located in a narrow zone of the stratum granulosum, comprising of peripheral and integral proteins that are necessary for connecting neighboring cells and providing barrier functions. Keratinocytes produce a range of host defense peptides (HDPs) that protect our body by inactivating pathogens, and exhibit various immunomodulatory activities. For instance, cathelicidin LL-37 directly participates in wound healing, promotes keratin...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Toshihiro Akiyama, Francois Niyonsaba, Toan The Nguyen, Rithee Smithrithee, Chanisa Kiatsurayanon, Hiroko Ushio, Ko Okumura, Hideoki Ogawa, Shigaku Ikeda Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

An ErbB receptor mediated AP-1 regulatory network is modulated by STAT3 and c-MYC during calcium dependent keratinocyte differentiation
A calcium gradient in skin epidermis is known to regulate epidermal differentiation. In cultures of human epidermal keratinocytes (NHEK), induction of calcium-dependent differentiation is associated with phosphorylation of ErbB receptors, including the epidermal growth factor receptor (EGFR). The activation of EGFR triggers the induction of activator protein 1 (AP-1) proteins necessary for keratinocyte terminal differentiation. Interestingly, an in vitro long-term calcium treatment revealed the activation of different ErbB receptors with different timings, which is consistent with the differential localization of each rece...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Yuko Saeki, Takeshi Nagashima, Shuhei Kimura, Mariko Okada-Hatakeyama Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Topically applied γ-linolenic acid restores corneocyte-bound ω-hydroxy ceramides and ameliorates atopic dermatitis-like symptoms in mice
In this study, we examined the effects of topical application of highly purified γ-linolenic acid (GLA)-containing ointment on the depleted ceramide levels and pruritic dermatitis. To induce AD-like symptoms, HR-1 hairless mice were fed a special diet for 8-12 weeks. GLA (certified purity >99%) was mixed with white petrolatum before the use. After dermatitis was established, GLA (5mg/200mg/animal/day) was applied to the skin for 7 days. Special diet-fed mice exhibited AD-like symptoms; including red scaly skin, decreased skin hydration, increased transepidermal water loss, and prolonged scratching. Histological examinatio...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Masanori Fujii, Hiroyuki Matsuda, Takahiro Toda, Susumu Ohya, Takeshi Nabe Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Sphingoid base composition influences normal function, and dictates altered lamellar bilayer function in atopic dermatitis
We demonstrated here first that alterations not only in ceramides, but also in sphingoid base profiles (i.e., sphingosine to sphinganine ratio), occur in the stratum corneum of AD mice, a model (repeated hapten [oxazolone] challenge) that faithfully replicates human AD. We assessed the potential consequences of altered sphingoid base content for barrier dysfunction in AD using unilamellarvesicles (LV), comprised of major stratum corneum lipids (ceramide, free fatty acids, and cholesterol), with either AD (AD-LV) or normal-LV (normal-LV) levels of sphingosine and/or sphinganine, encapsulating carboxyfluorescein. In response...
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Nicolas Loiseau, Yasuko Obata, Sam Moradian, Saeko Yoshino, Kenichi Aburai, Kozo Takayama, Kazutami Sakamoto, Peter_M. Elias, Walter_M. Holleran, Yoshikazu Uchida Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research

Effects of host defense peptides human β-defensins on skin tight junction barrier
Conclusion] In summary, our study implies that hBDs play a role in improving the skin tight junction barrier. The mechanism by which HDPs function remains to be elucidated.
Source: Journal of Dermatological Science - December 20, 2012 Category: Dermatology Authors: Chanisa Kiatsurayanon, Francois Niyonsaba, Rithee Smithrithee, Toan The Nguyen, Toshihiro Akiyama, Hiroko Ushio, Shigaku Ikeda, Ko Okumura, Hideoki Ogawa Tags: Abstracts from the 37th Annual Meeting of the Japanese Society for Investigative Dermatology Source Type: research