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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 32.

Initial Metformin or Sulfonylurea Exposure and Cancer Occurrence among Patients with Type 2 Diabetes Mellitus
ConclusionThis study provides evidence that cancer incidence in the first few years after starting metformin or sulfonylurea therapy in type 2 diabetes patients is not much affected by choice of hypoglycemic drug class.
Source: Diabetes, Obesity and Metabolism - November 9, 2012 Category: Endocrinology Authors: Hong Qiu, George G. Rhoads, Jesse A. Berlin, Stephen W. Marcella, Kitaw Demissie, Ph. D Tags: Original Paper Source Type: research

Compound heterozygous mutations (p.Leu13Pro and p.Tyr294*) associated with factor VII deficiency cause impaired secretion through ineffective translocation and extensive intracellular degradation of factor VII
Conclusions: These findings demonstrate that both mutant FVIIs impaired secretion through ineffective translocation to and retention in ER with extensive intracellular degradation, resulting in an insufficient phenotype.
Source: Thrombosis Research - November 9, 2012 Category: Hematology Authors: Keijiro Suzuki, Takeshi Sugawara, Yoji Ishida, Akira Suwabe Tags: Coagulation and Fibrinolysis Source Type: research

Association between variation in ADAMTS13 and aneurysmal subarachnoid hemorrhage
Dear Editors, ADAMTS13 (A Disintegrin-like and Metalloprotease with Thrombospondin Type 1 Motif, 13) is a protease encoded by ADAMTS13 on chromosome 9q34. The main role of ADAMTS13 is to cleave von Willebrand Factor (VWF), and thereby inhibit thrombus formation at sites of vascular injury. ADAMTS13 is primarily synthesized by hepatic stellate cells, but also by endothelial cells and platelets . ADAMTS13 circulates in plasma but can also bind to endothelial cells where it exhibits enhanced proteolytic activity towards VWF, as compared to circulating ADAMTS13 .
Source: Thrombosis Research - November 9, 2012 Category: Hematology Authors: Ellen Hanson, Sandra Olsson, Bilâl Bayazit, Ludvig Z. Csajbok, Karin Nylén, Bengt Nellgård, Christina Jern Tags: Letters to the Editors-in-Chief Source Type: research

Three-month mortality rate and clinical predictors in patients with venous thromboembolism and cancer. Findings from the RIETE registry
Abstract: Patients with venous thromboembolism (VTE), and particularly those with cancer, are at increased risk of recurrences, major bleeding, and short- / medium-term mortality.Data from 35,539 patients (6,075 of these with cancer), presenting with symptomatic VTE in the previous three months and enrolled in the worldwide RIETE registry, were evaluated to assess overall and pulmonary embolism (PE)-related mortality, and their potential predictors, with particular focus on patients with cancer.Overall 3-month mortality in the total RIETE population was 7.9%, and death was considered PE-related in 1.4%. Significantly more ...
Source: Thrombosis Research - November 9, 2012 Category: Hematology Authors: Gualberto Gussoni, Stefania Frasson, Micaela La Regina, Pierpaolo Di Micco, Manuel Monreal, for the RIETE Investigators Tags: Clinical Studies Source Type: research

Chinese medicine formula “Weikang Keli” induces autophagic cell death on human gastric cancer cell line SGC-7901
In this study, we found that Weikang Keli could induce patterns of autophagy in SGC-7901 cells, including intracellular vacuole formation, microtubule-associated protein 1 light chain 3 (LC3) conversion. Hoechst 33258 staining and Western blot analysis of apoptosis-related proteins showed that WK induced SGC-7901 cell death was not through apoptosis. In vivo study also revealed that i.g. administration of Weikang Keli once a day for 25 days could significantly reduce tumor volumes by about 50%. Collectively, the current data indicated that Weikang Keli induced gastric cancer cell death by autophagy effects.
Source: Phytomedicine - November 9, 2012 Category: Drugs & Pharmacology Authors: Jiege Huo, Fengxia Qin, Xueting Cai, Jianming Ju, Chunping Hu, Zhigang Wang, Wuguang Lu, Xiaoning Wang, Peng Cao Tags: Oncology and Hematology Source Type: research

Identification of thymus and activation-regulated chemokine (TARC/CCL17) as a potential marker for early indication of disease and prediction of disease activity in drug-induced hypersensitivity syndrome (DIHS)/drug rash with eosinophilia and systemic symptoms (DRESS)
Conclusion: Serum TARC levels may be associated with the initial presentation of DIHS as well as disease activity during the course. Thus, they could be useful as an indicator for early diagnosis and assessment of disease activity in DIHS. CD11c+ dendritic cells may be the main source of TARC in patients with DIHS.
Source: Journal of Dermatological Science - November 9, 2012 Category: Dermatology Authors: Kohei Ogawa, Hironori Morito, Ayako Hasegawa, Natsuko Daikoku, Fumi Miyagawa, Aiko Okazaki, Takaya Fukumoto, Nobuhiko Kobayashi, Takahiko Kasai, Hideaki Watanabe, Hirohiko Sueki, Masafumi Iijima, Mikiko Tohyama, Koji Hashimoto, Hideo Asada Tags: Regular articles Source Type: research

A naked RNA heptamer targeting the human Bcl-2 mRNA induces apoptosis of HL60 leukemia cells
Highlights: ► TRUE gene silencing is a gene control technology based on sgRNA-directed specific mRNA cleavage by tRNase ZL. ► Behavior of heptamer-type sgRNA was investigated to find therapeutic sgRNAs to cure hematological malignancies. ► A heptamer, mh1(Bcl-2), which targets the human Bcl-2 mRNA, can be taken up by cells without any transfection reagents. ► mh1(Bcl-2) can induce apoptosis of HL60 leukemia cells. ► In mouse xenograft experiments, a median survival of the mh1(Bcl-2)-treated mice is longer than that of the control mice.Abstract: tRNase ZL-utilizing efficacious gene silencing is a gene control tech...
Source: Cancer Letters - November 9, 2012 Category: Cancer & Oncology Authors: Masayuki Takahashi, Reyad A. Elbarbary, Aiko Nakashima, Mayumi Abe, Norihiro Watanabe, Miwako Narita, Masuhiro Takahashi, Masato Tamura, Tetsuo Yoshida, Masayuki Nashimoto Tags: Research Articles Source Type: research

Successful Treatment of Hepatic Sinusoidal Obstructive Syndrome after Hematopoietic Stem Cell Transplantation in a Child Using Recombinant Thrombomodulin.
Abstract No abstract available. PMID: 23147560 [PubMed - as supplied by publisher]
Source: Acta Haematologica - November 9, 2012 Category: Hematology Authors: Yamamoto S, Yagawa A, Toyama D, Akiyama K, Hayashi M, Mabuchi M, Shimizu T, Ikeda H, Isoyama K Tags: Acta Haematol Source Type: research

Analysis of the genetic variants of glucose-6-phosphate dehydrogenase in inhabitants of the 4th Nile cataract region in Sudan.
In this study, we aimed to analyze the frequency of two single-nucleotide polymorphisms (G202A and A376G) present in two local tribes of Sudanese Arabs from the region of the 4th Nile cataract in Sudan, the Shagia and Manasir. The polymorphisms in G6PD were analyzed in 217 individuals (126 representatives of the Shagia tribe and 91 of the Manasir tribe). Real-time PCR and RFLP-PCR were utilized to analyze significant differences in the prevalence of alleles and genotypes. The 202A G6P allele frequency was 0.7%, whereas the G202 variant was found in 93.3% of cases. The AA, GA, and GG genotype frequencies for the A376G G6PD ...
Source: Blood Cells, Molecules and Diseases - November 9, 2012 Category: Hematology Authors: Kempinska-Podhorodecka A, Knap O, Drozd A, Kaczmarczyk M, Parafiniuk M, Parczewski M, Milkiewicz M Tags: Blood Cells Mol Dis Source Type: research

Effects of oral administration of Bacillus thuringiensis as spore-crystal strains Cry1Aa, Cry1Ab, Cry1Ac or Cry2Aa on hematologic and genotoxic endpoints of Swiss albino mice.
Abstract Bacillus thuringiensis (Bt) as spore-crystal proteins (Cry) is widely used in bioinsecticide formulations to protect different crops by biological control. Bt insecticides are much more specific than broad-spectrum synthetic chemical insecticides, but after the advent of genetically modified plants expressing δ-endotoxins (transgenic crops), the bioavailability of Cry proteins has increased, and for biosafety reasons their adverse effects should be studied, mainly for non-target organisms. Thus, we evaluated, in Swiss albino mice, the hematotoxicity and genotoxicity of four strains of Bt spore-crystals, C...
Source: Food and Chemical Toxicology - November 9, 2012 Category: Food Science Authors: Mezzomo BP, Miranda-Vilela AL, de Souza Freire I, Barbosa LC, Portilho FA, Lacava ZG, Grisolia CK Tags: Food Chem Toxicol Source Type: research

Treatment and outcomes for chronic myelomonocytic leukemia compared to myelodysplastic syndromes in older adults.
Conclusions Chronic myelomonocytic leukemia is less frequently treated in older adults and is associated with worse outcomes, even after controlling for baseline patient characteristics and selected treatments. Our data suggest the need for continued evaluation of the biologic differences between these diseases and clinical trials targeting chronic myelomonocytic leukemia. PMID: 23144192 [PubMed - as supplied by publisher]
Source: Haematologica - November 9, 2012 Category: Hematology Authors: Zandberg DP, Huang TY, Ke X, Baer MR, Gore SD, Weiss Smith S, Davidoff AJ Tags: Haematologica Source Type: research

Everolimus in combination with rituximab induces complete responses in heavily pretreated diffuse large B-cell lymphoma.
Conclusion Everolimus in combination with rituximab is well tolerated and demonstrates activity in relapsed diffuse large B cell lymphoma. Further studies of this combination are warranted. clinicaltrials.gov identifier : NCT00869999 Key Words: Diffuse large B cell lymphoma, everolimus, mTOR, rituximab, non-Hodgkin lymphoma. PMID: 23144193 [PubMed - as supplied by publisher]
Source: Haematologica - November 9, 2012 Category: Hematology Authors: Barnes J, Jacobsen E, Feng Y, Freedman A, Hochberg E, Lacasce A, Armand P, Joyce R, Sohani A, Rodig S, Neuberg D, Fisher D, Abramson J Tags: Haematologica Source Type: research

Association between B-cell receptor responsiveness and disease progression in B-cell chronic lymphocytic leukemia: results from single cell network profiling studies.
Conclusions. These findings support the utility of the single cell network profiling assay in elucidating signaling perturbations with the potential for the development of a clinically useful prognostic test in patients with early stage B-cell chronic lymphocytic leukemia. These data support the clinical relevance of B-cell receptor signaling in B-cell chronic lymphocytic leukemia, and suggest a key role of ERK activation in the physiopathology of this leukemia. PMID: 23144194 [PubMed - as supplied by publisher]
Source: Haematologica - November 9, 2012 Category: Hematology Authors: Cesano A, Perbellini O, Evensen E, Chu CC, Cioffi F, Ptacek J, Damle RN, Chignola R, Cordeiro J, Yan XJ, Hawtin RE, Nichele I, Ware JR, Cavallini C, Lovato O, Zanotti R, Rai KR, Chiorazzi N, Pizzolo G, Scupoli MT Tags: Haematologica Source Type: research

Splenectomy as a curative treatment for immune thrombocytopenia: a retrospective analysis of 233 patients with a minimum follow-up of 10 years.
Conclusions. Splenectomy achieved a long-term stable responses in around 60% of cases. Complications mainly affected non-responding patients and were fatal in a minority of the cases. PMID: 23144195 [PubMed - as supplied by publisher]
Source: Haematologica - November 9, 2012 Category: Hematology Authors: Vianelli N, Palandri F, Polverelli N, Stasi R, Joelsson J, Johansson E, Ruggeri M, Zaja F, Cantoni S, Catucci AE, Candoni A, Morra E, Björkholm M, Baccarani M, Rodeghiero F Tags: Haematologica Source Type: research

Central nervous system recurrence of systemic lymphoma in the era of stem cell transplantation - an international primary central nervous system lymphoma study group project.
Conclusions. Despite the availability of autologous stem cell transplantation for patients with central nervous system progression or relapse of systemic lymphoma, prognosis is still poor. Long-term survival is, however, possible and more likely in patients able to undergo stem cell transplantation. PMID: 23144196 [PubMed - as supplied by publisher]
Source: Haematologica - November 9, 2012 Category: Hematology Authors: Bromberg JE, Doorduijn JK, Illerhaus G, Jahnke K, Korfel A, Fischer L, Fritsch K, Kuittinen O, Issa S, van Montfort C, van den Bent MJ Tags: Haematologica Source Type: research

PML-RARα cooperates with SOX4 in acute myeloid leukemia development in mice.
Abstract Acute promyelocytic leukemia is characterized by a chromosomal translocation involving the retinoic acid receptor alpha gene. To identify cooperating pathways to leukemogenesis we crossed MRP8-PML/RARA transgenic mice with BXH-2 mice, which harbor an endogenous murine leukemia virus that causes acute myeloid leukemia. Approximately half of leukemias that arose in this cross showed features of acute promyelocytic leukemia. We identified 22 proviral insertions sites in acute promyelocytic-like leukemias and focused our analysis on insertion at Sox4, a HMG box transcription factor. Using a transplant model, c...
Source: Haematologica - November 9, 2012 Category: Hematology Authors: Omidvar N, Maunakea ML, Jones L, Sevcikova S, Yin B, Himmel KL, Tennant TR, Le Beau MM, Largaespada DA, Kogan SC Tags: Haematologica Source Type: research

Lenalidomide as salvage treatment for multiple myeloma relapsing after allogeneic hematopoietic stem cell transplantation: a report from the SFGM-TC.
Conclusion. Lenalidomide can induce high response rates in myeloma relapsing after allogeneic stem cell transplantation at least in part by triggering an allogeneic anti-myeloma response. Induced graft-versus-host disease has to be put in balance with the potential benefit on disease control. Further immunologic studies would have an interest in understanding lenalidomide immunomodulatory activity in vivo. Key words: Allogeneic hematopoietic stem cell transplantation, graft versus host disease, IMiDs, Multiple Myeloma. PMID: 23144198 [PubMed - as supplied by publisher]
Source: Haematologica - November 9, 2012 Category: Hematology Authors: Coman T, Bachy E, Michallet M, Socie' G, Uzunov M, Bourhis JH, Lapusan S, Brebion A, Vigouroux S, Maury S, Francois S, Huynh A, Lioure B, Yakoub-Agha I, Hermine O, Milpied N, Mohty M, Rubio MT Tags: Haematologica Source Type: research

H-Net, The European Network for Harmonization of Training in Hematology, and its Policy.
Abstract Not available. PMID: 23144199 [PubMed - as supplied by publisher]
Source: Haematologica - November 9, 2012 Category: Hematology Authors: Duyvene' de Wit T, Hellström-Lindberg E, Jaeger U, Evans-Jones G, Smand C Tags: Haematologica Source Type: research

Melphalan, lenalidomide and dexamethasone for the treatment of AL amyloidosis: results of a phase II trial.
In conclusion, this combination is associated with significant myelosuppression leading to dose modifications and producing minor hematologic responses in AL amyloidosis. http://clinicaltrials.gov/ct2/show/NCT00679367. PMID: 23144200 [PubMed - as supplied by publisher]
Source: Haematologica - November 9, 2012 Category: Hematology Authors: Sanchorawala V, Patel JM, Sloan JM, Shelton AC, Zeldis JB, Seldin DC Tags: Haematologica Source Type: research

Myeloablative chemotherapy for chemo-sensitive recurrent follicular lymphoma: potential benefit in second relapse.
Conclusions: high-dose therapy with autologous stem cell rescue with BEAM appears to be equally effective in second or third remission of follicular lymphoma. Key-words: autologous stem cell rescue, BEAM, follicular lymphoma. PMID: 23144201 [PubMed - as supplied by publisher]
Source: Haematologica - November 9, 2012 Category: Hematology Authors: Montoto S, Matthews J, Greaves P, Lillington D, Anderson D, Gribben JG, Lister TA Tags: Haematologica Source Type: research

A novel tylophorine analog W-8 up-regulates forkhead boxP3 expression and ameliorates murine colitis.
Abstract Tylophorine and analogs are phenanthroindolizidine alkaloids, several of which have been reported to have anticancer, antiviral, and anti-inflammatory properties. However, their function in the immune system remains widely unknown. Transcription factor Foxp3 is critical for the development and function of Treg, which down-regulates the immune system and maintains tolerance to self-antigens. In the present study, we defined a novel tylophorine analog, W-8, enhanced TGF-β-induced Foxp3 expression at the mRNA and the protein levels. Interestingly, W-8 synergistically increased the level of TGF-β-induced p-S...
Source: Journal of Leukocyte Biology - November 9, 2012 Category: Hematology Authors: Meng X, Zhang Y, Jia Z, Huo X, He X, Tian G, Wu M, Wang Z, Zhou X, Xiong S, Gao X, Wu Z, Han J, Zhao L, Wang P, Hong Z, Wang Q, Yin Z Tags: J Leukoc Biol Source Type: research

Erythrocyte aggregation portends worse outcomes in unstable angina patients undergoing percutaneous coronary interventions.
Conclusions: Elevated EA portends worse outcomes in UAP patients undergoing PCI. PMID: 23144163 [PubMed - as supplied by publisher]
Source: Clinical Hemorheology and Microcirculation - November 9, 2012 Category: Hematology Authors: Arie S, Yaron A, Eran LR, Amir H, Gad K, Miri R, Ariel F, Ruth CM, Galit A, Shlomo B, Shmuel B Tags: Clin Hemorheol Microcirc Source Type: research

Immunotactoid glomerulopathy: clinicopathologic and proteomic study
Conclusions Hematologic malignancy, particularly lymphoma, is not uncommon in ITG. ITG appears to have a better prognosis than other paraprotein-related renal lesions, with a half of patients expected to recover kidney function with immunosuppressive therapy or chemotherapy. The proteomic profile of ITG is consistent with deposition of monotypic immunoglobulins and activation of the classical and terminal pathway of complement.
Source: Nephrology Dialysis Transplantation - November 9, 2012 Category: Urology & Nephrology Authors: Nasr, S. H., Fidler, M. E., Cornell, L. D., Leung, N., Cosio, F. G., Sheikh, S. S., Amir, A. A., Vrana, J. A., Theis, J. D., Dogan, A., Sethi, S. Tags: Chronic Kidney Disease Source Type: research

Targeted drug delivery for cancer therapy: the other side of antibodies
Therapeutic monoclonal antibody (TMA) based therapies for cancer have advanced significantly over the past two decades both in their molecular sophistication and clinical efficacy. Initial development efforts focused mainly on humanizing the antibody protein to overcome problems of immunogenicity and on expanding of the target antigen repertoire. In parallel to naked TMAs, antibody-drug conjugates (ADCs) have been developed for targeted delivery of potent anti-cancer drugs with the aim of bypassing the morbidity common to conventional chemotherapy. This paper first presents a review of TMAs and ADCs approved for clinical u...
Source: Journal of Hematology and Oncology - November 9, 2012 Category: Hematology Authors: Michael FirerGary Gellerman Source Type: research

Inhibition of the ecto-beta subunit of F1F0-ATPase inhibits proliferation and induces apoptosis in acute myeloid leukemia cell lines
Conclusions: These findings indicate that ectopic expression of ATPase beta subunit may be a tumor-associated antigen in hematological malignancies. The F1F0 ATPase beta subunit provides a potential target for immunotherapy in AML and hematological malignancies.
Source: Journal of Experimental and Clinical Cancer Research - November 9, 2012 Category: Cancer & Oncology Authors: Zhao Wen-LiWang JianTao Yan-FangFeng XingLi Yan-HongZhu Xue-MingZhang MinNi JianPan Jian Source Type: research

NOTCH1 and SF3B1 mutations can be added to the hierarchical prognostic classification in chronic lymphocytic leukemia
isler & R Rosenquist
Source: Leukemia - November 9, 2012 Category: Hematology Authors: L MansouriN CahillR GunnarssonK E SmedbyE TjönnfjordH HjalgrimG JuliussonC GeislerR Rosenquist Source Type: research

Anatomic, Hematologic, and Biochemical Features of C57BL/6NCrl Mice Maintained on Chronic Oral Corticosterone.
Abstract Metabolic syndrome is a condition that typically includes central obesity, insulin resistance, glucose intolerance, dyslipidemia, and hypertension. Disruption of the hypothalamic-pituitary-adrenal axis, a regulator of corticosterone secretion, occurs in some cases of metabolic syndrome and obesity, and Cushing hypercortisolemia is associated with obesity and metabolic disorders. We therefore assessed anatomic and clinical pathology in C57BL/6NCrl mice to evaluate the effects of chronic corticosterone in the drinking water at doses of 25, 50, and 100 μg/mL for 25 d. Treated mice developed obesity, glucose ...
Source: Comparative Medicine - November 8, 2012 Category: Zoology Authors: Cassano AE, White JR, Penraat KA, Wilson CD, Rasmussen S, Karatsoreos IN Tags: Comp Med Source Type: research

Meningoencephalitis Due to Listeria monocytogenes in a Pregnant Rhesus Macaque (Macaca mulatta).
Abstract We here report a spontaneous case of meningoencephalitis due to Listeria monocytogenes in an adult primiparous rhesus macaque (Macaca mulatta) during an outbreak of listeriosis in an outdoor enclosure. Clinical signs included tremors, abnormal posture, and altered mental status. Hematology and analyses of cerebrospinal fluid were consistent with bacterial infection. Pure cultures of L. monocytogenes were recovered from the placenta-abortus, cerebrospinal fluid, and brain tissue. The macaque did not respond to treatment and was euthanized. Histopathologic examination of the brain revealed acute meningoencep...
Source: Comparative Medicine - November 8, 2012 Category: Zoology Authors: Lemoy MJ, Lopes DA, Reader JR, Westworth DR, Tarara RP Tags: Comp Med Source Type: research

Gender-specific elimination of continuous-infusional 5-fluorouracil in patients with gastrointestinal malignancies: results from a prospective population pharmacokinetic study
Conclusions Gender-specific elimination of 5FU is supported by the present data and may partly explain the gender-specific association between DPYD risk variants and 5FU-specific toxicity. Content Type Journal ArticleCategory Original ArticlePages 1-10DOI 10.1007/s00280-012-2018-4Authors F. Mueller, Department of Internal Medicine, Cantonal Hospital, St. Gallen, SwitzerlandB. Büchel, Institute of Clinical Chemistry, University Hospital, Bern, SwitzerlandD. Köberle, Clinical Research Facility, Department of Medical Oncology and Hematology, Cantonal Hospital, Rorschacherstr. 95, 9007 St. Gallen, Swit...
Source: Cancer Chemotherapy and Pharmacology - November 8, 2012 Category: Cancer & Oncology Tags: Cancer Chemotherapy and Pharmacology Source Type: research

Hemoparasite and hematological parameters in Nile tilapia
Abstract The accelerated growth of finfish aquaculture has resulted in a series of health problems, including blood disorders by hemoparasites; there are scarce studies about these agents and their impact in actual intensive farming. The aim of this study was to identify and quantify the hemoparasites present in monocytes and erythrocytes in the blood of tilapia and their correlation with the hematological profile. Blood samples were collected from caudal vessels of 15 cage-reared Nile tilapia (Oreochromis niloticus), with 70 ± 10 g weight on average, from Itambaracá Municipality, Parana State, Bra...
Source: Comparative Clinical Pathology - November 8, 2012 Category: Pathology Tags: Comparative Clinical Pathology Source Type: research

Profound neurological injury in a patient with atypical hemolytic uremic syndrome
Content Type Journal ArticleCategory Letter to the EditorPages 1-2DOI 10.1007/s00277-012-1615-yAuthors Galena Salem, Department of Internal Medicine, The Ohio State University Comprehensive Cancer Center, Starling Loving Hall, Room 365, 320 West 10th Avenue, Columbus, 43210 USAJoseph M. Flynn, Division of Hematology, The Ohio State University Comprehensive Cancer Center, B301 Starling Loving Hall, 320 West 10th Avenue, Columbus, 43210 USASpero R. Cataland, Division of Hematology, The Ohio State University Comprehensive Cancer Center, A361 Starling Loving Hall, 320 West 10th Avenue, Columbus, 43210 USA Journal An...
Source: Annals of Hematology - November 8, 2012 Category: Hematology Tags: Annals of Hematology Source Type: research

New missense homozygous mutation (Q270Ter) in the pyrimidine 5′ nucleotidase type I-related gene in two Indian families with hereditary non-spherocytic hemolytic anemia
Content Type Journal ArticleCategory Letter to the EditorPages 1-3DOI 10.1007/s00277-012-1616-xAuthors Prashant Warang, Department of Haematogenetics, National Institute of Immunohaematology, Indian Council of Medical Research, K.E.M. Hospital Campus, 13th Floor, New Multistoried Building, Parel, Mumbai, 400012 IndiaPrabhakar Kedar, Department of Haematogenetics, National Institute of Immunohaematology, Indian Council of Medical Research, K.E.M. Hospital Campus, 13th Floor, New Multistoried Building, Parel, Mumbai, 400012 IndiaRakhee Kar, Department of Clinical Hematology, Institute of Liver and Biliary Sciences, Vasan...
Source: Annals of Hematology - November 8, 2012 Category: Hematology Tags: Annals of Hematology Source Type: research

Freezing does not decrease carbon monoxide-mediated hypercoagulation and hypofibrinolysis in human plasma
Carbon monoxide (CO) has been demonstrated to enhance coagulation and attenuate fibrinolysis in vitro and in vivo. Hemostasis is affected by CO interactions with key heme-modulated molecules. We wished to determine whether freezing would affect CO-mediated changes in coagulation/fibrinolysis in plasma in anticipation of collecting samples both within our institution and from collaborating centers. Plasma was exposed to CO by addition of 0–100 μmol/l tricarbonyldichlororuthenium (II) dimer, with a portion of plasma immediately frozen at −80°C. Unfrozen plasma was subjected to thrombelastographic analysis following t...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Technical Reports Source Type: research

Diagnostic ultrasound activates pure prekallikrein
Diagnostic ultrasound activates the contact phase of human coagulation. This has been seen in human blood or plasma or with purified factor 12. The present work aimed to quantify a possibly triggering action of ultrasound on purified prekallikrein, the second of the two main triggers of the intrinsic hemostasis cascade. Either 2.7 μg/ml human prekallikrein or for control 1 μg/ml kallikrein in 26% glycerol – 0.54% NaCl–10.6 mmol/l Na3 citrate pH 7.4, in emptied polypropylene coagulation monovettes (Sarstedt) were exposed to diagnostic ultrasound (Siemens Acouson Antares, 5 MHz, 0.6 TIB, 0.6 TIS) for 0–5 min ...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Technical Reports Source Type: research

Drug-induced thrombocytopenia: a less known interaction
A 57-year-old woman with a left frontal lobe tumor was started on seizure prophylaxis with phenytoin and dexamethasone while awaiting elective surgery for tumor excision. Within a week, she developed a rash all over her body secondary to phenytoin hypersensitivity and her platelet counts decreased progressively to as low as 20 000/μl. Phenytoin was discontinued and she was given intravenous immunoglobulin for 2 days. She had progressive recovery of the platelet count to her baseline over the next 6 days. This case of phenytoin-induced thrombocytopenia emphasizes the importance of recognizing thrombocytopenia secondary t...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Case Reports Source Type: research

The dilemma of inherited dysfibrinogenemia during pregnancy
We present the case of a 20-year-old woman at 7 weeks of pregnancy with a history of two first-trimester spontaneous abortions and a family history of thrombotic events. Her testing revealed evidence of dysfibrinogenemia, necessitating multidisciplinary management planning including Hematology, OB-GYN, Maternal-Fetal Medicine, Blood Bank Services and Anesthesia. Antenatal care included a combination of intravenous fibrinogen infusions to maintain fibrinogen levels above 100 mg/dl and anticoagulation with low molecular weight heparin. She had an uneventful full-term delivery and continued fibrinogen infusions and thrombop...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Case Reports Source Type: research

Intentional low-molecular-weight heparin overdose: a case report and review
We report the case of a 34-year-old woman, who intentionally overdosed on enoxaparin (Lovenox) in a suicide attempt. Her initial antifactor Xa activity level was 8.3 IU/ml, the highest level reported in the literature to date. She was initially managed conservatively, however, within 24 h of admission she developed evidence of acute blood loss. Protamine sulfate and three doses of recombinant activated factor VII (rFVIIa) were administered in an effort to control bleeding. We report the effects of these measures and review the literature to date. Our study is one of the first to graph in-vivo antifactor Xa activity lev...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Case Reports Source Type: research

Successful use of fondaparinux in a child with heparin-induced thrombocytopenia
We report a case of HIT developing with a background of prothrombotic genetic risk factors and antiphospholipid syndrome. This case study highlights several difficulties in pediatric HIT cases.
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Case Reports Source Type: research

Novel microfluidic platform for automated lab-on-chip testing of hypercoagulability panel
Current methods for hypercoagulability panel testing require large blood volumes and long turn-around testing times. A novel microfluidic platform has been designed to perform automated multiplexed hypercoagulability panel testing at near patient, utilizing only a single droplet of blood sample. We test the hypothesis that this novel platform could be utilized to perform specific multiplexed ELISA-based hypercoagulability panel testing for antithrombin III, protein C, protein S and factor VIII antigens, as well as anticardiolipin/human anti-β2-glycoprotein-1 IgG antibodies – on blood samples. Sandwich ELISA was modified...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research

Short-term effects of aspirin and clopidogrel on mean platelet volume among patients with acute coronary syndromes. A single-center prospective study
Mean platelet volume (MPV) has been correlated with platelet reactivity, thus its changes might be used to monitor the effects of antiplatelet therapy. However, no data have been reported on platelet remodelling after antiplatelet therapy. The aim of the current study was to investigate the short-term effects of oral antiplatelet therapy on MPV. Our population is represented by 62 consecutive patients with acute coronary syndrome (ACS), who did not receive GpIIb-IIIa inhibitors. We measured MPV before starting dual antiplatelet therapy, at day 1, day 2, and day 3–5 from starting adjunctive antiplatelet therapy. We additi...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research

Outcomes in women receiving low-molecular-weight heparin during pregnancy
To assess the rate and type of maternal and infant complications among pregnant women receiving low-molecular-weight heparin (LMWH). Retrospective study of pregnant women on LMWH referred to two university hematology clinics from January 2001 to December 2010. We recorded the number of pregnancies, indication, dose and dose adjustments for LMWH, pregnancy outcomes (live births, maternal and infant complications) and side effects of LMWH. There were 89 pregnancies in 76 women. The most common indication for LMWH was a history of adverse outcome of pregnancy associated with thrombophilia. LMWH was adjusted in 75 and 45% of p...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research

Coagulation factors and antithrombin levels in young and elderly subjects in Pakistani population
This study aimed to measure the plasma levels of coagulation factors and anticoagulant levels in young and elderly to observe the effect of age on haemostatic system. Ninety healthy individuals, both men and women were divided into two groups on the basis of age. Group I included participants of less than 40 years of age, whereas, group II comprised of participants more than 60 years of age. Fibrinogen activity was assessed by using Clauss technique. Coagulation factor VII, and factor VIII activity by corresponding one stage assay based on prothrombin time and activated partial thromboplastin time. Antithrombin III was mea...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research

Clinical significance of the mixing test in laboratory diagnoses of lupus anticoagulant: the fate of the mixing test in integrated lupus anticoagulant test systems
The mixing test is used to determine the presence of inhibitors in laboratory diagnoses of lupus anticoagulant. Updated international guidelines state that an integrated lupus anticoagulant test system does not require the mixing test; an appraisal of the mixing tests in integrated lupus anticoagulant test systems is, therefore, required. We investigated the clinical relevance of mixing tests by using the best cutoff value of the mixing test through thrombotic risk analysis. A retrospective analysis was performed on 525 specimens with positive screening tests by using two integrated lupus anticoagulant tests: diluted Russe...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research

Comparative study of the reactivity of natural and mutated streptokinase with total antistreptokinase antibodies in human sera
Streptokinase is widely used as an anticoagulant drug for the treatment of heart attacks. Because of antibody production against injected drug in individuals consuming streptokinase and causing allergic reactions, streptokinase treatment effects become neutral. Recombinant mutant type of streptokinase was prepared by removing of 42 amino acids from the C terminal region. ELISA plates were coated by natural and mutant streptokinase as antigen. Ninety-six normal serum samples as well as 27 streptokinase consumer serum samples (patients with acute myocardial infraction) were analyzed. The results showed that serum antibodies ...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research

Plasma tissue-type plasminogen activator increases fibrinolytic activity of exogenous urokinase-type plasminogen activator
The relationship between tissue-type plasminogen activator (tPA) and urokinase-type plasminogen activator (uPA) function is not fully understood. The aim of this study was to compare in vitro the fibrinolytic activity of tPA and uPA and evaluate their possible interaction. Blood coagulation and fibrinolysis were conducted by rotation thromboelastometry, whereas blood clotting was induced by CaCl2 and tissue factor and fibrinolysis additively by tPA and uPA. Effective concentration 50% of tPA and uPA fibrinolytic activity in blood was found to be 90 and 33 IU/ml relating to the units of activity established by manufacture...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research

Postmenopausal women have an increased maximal platelet reactivity compared to men despite dual antiplatelet therapy
Dual antiplatelet medication with acetylsalicylic acid (ASA) and clopidogrel is the main therapy for patients with stable coronary vessel disease (CVD) after percutaneous coronary intervention (PCI). Despite platelet inhibition subgroups of patients have been shown to exhibit an increase of risk for adverse cardiovascular events. The aim of our study was to elucidate the influence of sex on platelet reactivity in patients with CVD under medication with ASA and clopidogrel. Two hundred and thirty patients with CVD on combined therapy with ASA (100 mg/day) and clopidogrel (75 mg/day) were included into our study. These p...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research

Different cardiac tissue plasminogen activator release patterns by local stimulation of the endothelium and sympathetic nerves in pigs
This study demonstrates strong association between tPA and NE during SS and possibly two different sources or mechanisms for SS-induced tPA release.
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research

Should vitamin K antagonist therapy be started simultaneously with parenteral anticoagulation: a meta-analysis?
The objective of this study was to perform a meta-analysis on the evidence comparing early versus late initiation of VKA on the effectiveness and safety of anticoagulation. We searched for randomized controlled trials in Medline, EMBASE, Cochrane CENTRAL, IPA and ClinicalTrials.gov. Studies were included if they compared early initiation of VKA (within approximately 24 h) and late initiation (>4 days) of the onset of heparin therapy. Data were pooled using the Review Manager 5 software and the quality of evidence was appraised with Grading of Recommendations, Assessment, Development and Evaluation profiler. Five studies ...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research

Are low-molecular-weight heparins appropriately dosed in patients with CKD stage 3 to 5?
The aims of this study were to determine the serum antifactor Xa levels in patients with impaired glomerular filtration receiving low-molecular-weight heparins (LMWHs), monitor efficacy and complications during treatment, and determine the approach to dose adjustments of LMWH by using antifactor Xa levels in different levels of kidney dysfunction in a real world clinical practice. Patients with chronic kidney disease who had subcutaneous enoxaparin administration during hospitalization were included in this study. We obtained data on patient demographics, glomerular filtration rate, bleeding complications, and antifactor X...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research

Protein C polymorphism and susceptibility to PTE in China
Pulmonary thromboembolism (PTE) is a common clinical problem that is associated with substantial morbidity and mortality. We investigated the role of protein C polymorphism in patients with PTE in order to find out the correlation between its polymorphism and the susceptibility of the Chinese population to develop PTE. We used a case–control study design. Sixty-three consecutive patients with PTE were enrolled as the investigated group and 86 healthy people as the control group. Two novel polymorphisms, C/T at the position of 2405 and A/G at the position of 2418in the protein C gene promoter region were detected through ...
Source: Blood Coagulation and Fibrinolysis - November 8, 2012 Category: Hematology Tags: Original Articles Source Type: research