Laboratory MedicineThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

This page shows you the most recent publications within this specialty of the MedWorm directory.

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Calibration issues for nanoindentation experiments: Direct atomic force microscopy measurements and indirect methodsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This article discusses calibration issues for shallow depth nanoindentation experiments with Berkovich tips with respect to the accurate measurement of the diamond area function (DAF). For this purpose, two different calibration procedures are compared: (i) the direct measurement of the DAF through atomic force microscopy (AFM) imaging of the Berkovich tip at shallow depth and (ii) a novel indirect calibration method based on an iterative robust and converging scheme in which both reduced modulus and indentation hardness are simultaneously used. These results are obtained by indentation measurements on a standard specimen ...
Source: Microscopy Research and Technique - March 20, 2010 Category: Laboratory Medicine Authors: A.C. Barone, M. Salerno, N. Patra, D. Gastaldi, E. Bertarelli, D. Carnelli, P. Vena Source Type: journals

Disparity in estimated average glucose due to different hemoglobin A1c methods and hemoglobin S traitEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 48 (4): 571-572 (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

High specificity but low sensitivity of the cartilage oligomeric matrix protein (COMP) test in rheumatoid arthritis and osteoarthritisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 48 (4): 569-570 (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

A methodological reappraisal of total and high molecular weight adiponectin determination in human peripheral circulation: comparison of four immunometric assaysEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The results of this study contribute to a better understanding of the methodological features of the several ELISAs, and help in the evaluation and comparison of the relative results. Clin Chem Lab Med 2010;48:561–8. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Association of hyperhomocysteinemia with left ventricular dilatation and mass in human heartEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Hyperhomocysteinemia appears to be, at least in part, involved in a disproportional LV dilatation, where the ensuing hypertrophy is not sufficient to compensate for the increased wall stress. A potential mechanism is the hyperhomocysteinemia associated increase in oxidative stress that favors muscle fiber slippage. Clin Chem Lab Med 2010;48:555–60. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

C-reactive protein variants are not associated with susceptibility to stroke and stroke recurrenceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Although these variants and corresponding haplotypes in the CRP gene are associated with serum CRP concentrations, our study does not support that variants and corresponding haplotypes studied here have a major influence on risk of stroke and stroke recurrence. Therefore, we speculate that CRP is not a causal factor for stroke. Clin Chem Lab Med 2010;48:551–4. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Association of matrix metalloproteinase 9 genotypes and cardiovascular disease risk factors with serum matrix metalloproteinase 9 concentrations in Taiwanese individualsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The rs2274756-AA and rs3918242-TT genotypes, younger age, current smoking status and increased fasting plasma glucose, and fibrinogen concentrations were independently associated with high serum MMP-9 concentrations in Taiwanese individuals. Clin Chem Lab Med 2010;48:543–9. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Pregnancy-associated plasma protein-A as a marker for long-term mortality in patients with peripheral atherosclerosis: inconclusive findings from the Linz Peripheral Arterial Disease (LIPAD) studyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: In this study, PAPP-A was an independent predictor of 5-year all-cause mortality in patients with symptomatic PAD. However, based on the weak association between PAPP-A and outcome in our cohort, we consider PAPP-A measurements to not be useful in clinical practice for prognostic purposes in patients with PAD. Clin Chem Lab Med 2010;48:537–42. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Comparison of different depletion strategies for improving resolution of the human urine proteomeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 48 (4): 531-535 Abstract Background: Urine, being an ultrafiltrate of plasma, is a rich source for biomarker discovery. Since potential new disease markers are often present in low concentrations, a prefractionation/enrichment step could be useful in the discovery process. To enhance the detection of low-abundance proteins, three immuno-affinity depletion approaches were evaluated. Methods: To remove the most abundant proteins from a human urine sample, GenWay™ Spin IgY-12 kit, HPLC Agilent Hu-PL7 and a home-made column vs. human serum albumin [immuno-affinity column (IAC)] were...
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Correlation between glucose and bupivacaine levels in cerebrospinal fluid after spinal anesthesia: glycorrhachia as predictor for duration of sensory blockEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 48 (4): 523-530 Abstract Background: Prediction of the duration of motor block after injection of a local anesthetic into cerebrospinal fluid (CSF) would be a very useful tool in clinical practice. However, previous attempts have not shown conclusive results. In this work, glycorrhachia is demonstrated to be an adequate predictive parameter after spinal anesthesia using 0.5% hyperbaric bupivacaine. Methods: Two mL of local anesthetic through a continuous spinal catheter was administered to 40 patients. CSF was sampled at different time intervals from the onset of infusion to motor...
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Beware of carryover in modern chemistry analyzersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 48 (4): 519-521 Abstract Background: Random-access analyzers that employ dedicated probes may be affected by carryover. Sample carryover, involving analyte from one sample that is measured in a subsequent sample, is most often detected in measurement procedures that have wide reportable ranges. However, reagent carryover can be more difficult to detect as it may involve only one pair of measurement procedures. Methods: Our laboratory noted several patient samples with total cholesterol (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Simultaneous determination of serum tryptophan metabolites in patients with systemic lupus erythematosus by high performance liquid chromatography with fluorescence detectionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 48 (4): 513-517 Abstract Background: To provide a more comprehensive clinic marker of tryptophan (TRP) catabolism in patients with systemic lupus erythematosus (SLE), we developed a simple and efficient method that simultaneously measured serum TRP, kynurenine (KYN), and kynurenic acid (KYNA) using high performance liquid chromatography with fluorescence detection (HPLC-FD). Methods: A simple and specific high performance liquid chromatography (HPLC) method was developed for simultaneously quantitative determination of TRP, KYN and KYNA with fluorescence detection (FD) using progr...
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Clinical application of a lectin-antibody ELISA to measure fucosylated haptoglobin in sera of patients with pancreatic cancerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: While certain cases showed a discrepancy in fucosylated haptoglobin concentrations between the lectin-antibody ELISA and conventional lectin blotting, this novel type of lectin-antibody ELISA might be useful for a tumor marker for PC. Clin Chem Lab Med 2010;48:505–12. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Procalcitonin measurement in routine emergency medicine practice: comparison between two immunoassaysEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: A highly significant correlation was observed between the two automated assays. Procalcitonin concentrations obtained from both methods led to the same clinical interpretation. Clin Chem Lab Med 2010;48:501–4. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Evaluation of C-reactive protein and serum amyloid A in the detection of inflammatory and infectious diseases in childrenEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Measurement of CRP showed the best performance for indicating infectious or inflammatory processes. ESR and SAA had good concordance and yielded very similar results. CRP may be included in protocols for pediatric assessment in emergency departments. Clin Chem Lab Med 2010;48:493–9. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Low expression of human β-defensin 1 in duodenum of celiac patients is partially restored by a gluten-free dietEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Our data reinforce the evidence that hBD1 expression is greatly reduced in the duodenum of patients with active CD. It also strengthens the concept that reduced activity of immune peptides may predispose individuals to bacterial proliferation that contributes to the pathogenesis of CD. Clin Chem Lab Med 2010;48:489–92. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

FOXL2 mutations in Taiwanese patients with blepharophimosis, ptosis, epicanthus inversus syndromeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Our results expand the spectrum of FOXL2 mutations and confirm the mutation hotspot in FOXL2 in Taiwanese BPES patients. Clin Chem Lab Med 2010;48:485–8. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Genotyping mitochondrial DNA single nucleotide polymorphisms by PCR ligase detection reactionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The PCR-LDR mtDNA genotyping technique is simple, highly accurate, has high-throughput, and is cost-effective. Therefore, this method is applicable to mtDNA haplotyping in various applications. Clin Chem Lab Med 2010;48:475–83. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Performance evaluation of the Abbott RealTime HCV Genotype II for hepatitis C virus genotypingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The Abbott RealTime HCV Genotype II assay was very accurate and yielded results consistent with RFMP data. Although the assay has the advantages of automation and short turnaround time, we suggest that further improvements are necessary before it is used routinely in clinical practice. Efforts are needed to decrease cross reactivity among genotypes and to improve the ability to detect minor genotypes in mixed infections. Clin Chem Lab Med 2010;48:469–74. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Assessment of critical values policies in Italian institutions: comparison with the US situationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Accreditation according to international standards can decrease differences regarding the management of critical values across laboratories of different countries. However, the issues concerning critical limits should be debated and a consensus critical values list should be considered. Automated systems could offer improvements regarding some issues, such as who makes the notification, reducing the time spent in notification of critical values. Surveys for comparing and improving existing policies regarding critical values should be promoted at an international level. Clin Chem Lab Med 2010;48:461–8. (Sourc...
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Decision criteria for rational selection of homogeneous genotyping platforms for pharmacogenomics testing in clinical diagnosticsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This article reviews recent developments in homogeneous technologies for the genotyping of single nucleotide polymorphisms. Here, homogeneous methods essentially refer to “single-tube” assays performed in a liquid phase. For the appropriate choice of any method, several criteria must be considered: 1) detection of known genetic variations; 2) analytical performance including specificity, sensitivity and robustness of the method; 3) availability of large platforms and required equipment; 4) suitability of platforms and tests for routine diagnostics; 5) suitability for high throughput implementation. Content: This review...
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

State of the art in therapeutic drug monitoringEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of the post-analytical phase is the final report, which should include correct interpretation, as well as possible advice. Appropriate pharmacokinetic interpretation avoids unnecessary costs and leads to clinical benefits. Clin Chem Lab Med 2010;48:437–46. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Improving the post-analytical phaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 48 (4): 435-436 (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Molecular biology and genetics in clinical chemistry and laboratory medicineEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 48 (4): 431-434 (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - March 19, 2010 Category: Laboratory Medicine Source Type: journals

Effort to Map Human Microbiome Will Generate Useful New Clinical Lab Tests for PathologistsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human Microbiome Project is expected to trigger many new molecular diagnostic assays Meet the human microbiome, considered by some medical researchers to be the newest biomedical frontier. A major effort to map the human microbiome is expected to identify a significant number of new biomarkers that will be useful in both clinical pathology diagnostic tests and [...] (Source: Dark Daily)
Source: Dark Daily - March 19, 2010 Category: Laboratory Medicine Authors: jude Tags: Digital Pathology Laboratory News Laboratory Pathology bacteria bacteria autism research biomarker cancer clinical laboratory clinical laboratory news colitis Crohn’s dark daily news dark report news digestive disorder diseas Source Type: info

Bioluminescent imaging of reporter gene expression in the lungs of wildtype and model mice following the administration of PEG-stabilized DNA nanoparticlesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
DNA nanoparticles (DNPs) formed by compacting DNA with polyethyleneglycolylated poly-L-lysine are a nonviral vector shown to be safe and efficacious in animals and humans. To extend our capabilities of assessing the efficacy and duration of expression achieved by DNPs, we tested the utility of bioluminescent imaging (BLI) of transgene expression in wildtype and cystic fibrosis (CF) mouse models. We tested the effect of route of administration, mouse coat color, anesthesia, dose, and promoter sequence on the level and duration of expression. Furthermore, we investigated the correlation between imaging and direct analysis of...
Source: Microscopy Research and Technique - March 19, 2010 Category: Laboratory Medicine Authors: Assem G. Ziady, Maxwell Kotlarchyk, Laura Bryant, Matthew Mcshane, Zhenghong Lee Source Type: journals

Immunochemical or fluorescent labeling of vesicular subcellular fractions for microscopy imagingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a procedure for the labeling of membranous vesicular purified subcellular fractions, to image them, typically by confocal laser scanning microscopy. Being intracellular organelles, these fractions, once purified cannot be attached to glass slides as for cells. Fractions are labeled "in batch" without prior embedding or freezing. Each labeling step performed by passages of resuspension/centrifugation is followed by washings. Then samples are dispersed on the glass slides. Mammalian retinal rod outer segment disks, intact brain stem myelin vesicles, and brain synaptosomes were chosen, as these subcellular fractio...
Source: Microscopy Research and Technique - March 16, 2010 Category: Laboratory Medicine Authors: Isabella Panfoli, Daniela Calzia, Silvia Ravera, Paolo Bianchini, Alberto Diaspro Source Type: journals

“Do It Yourself” Dermatopathology Will Use Consumer’s Cell Phone ImagesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Company intends to use pattern recognition software to evaluate risk of skin cancer A “do it yourself” dermatopathology service for consumers is coming soon, according to Health Discovery Corporation (HDC) of Savannah, Georgia. The company is preparing to introduce a cell phone-based tool to help consumers recognize whether a mole or other skin lesion needs examination [...] (Source: Dark Daily)
Source: Dark Daily - March 15, 2010 Category: Laboratory Medicine Authors: jude Tags: Laboratory News Laboratory Pathology Uncategorized cell phone and health cell phone application clinical laboratory clinical pathology Clinical Pathology News dark daily news dark report new dermatology Dermatopathology digital ima Source Type: info

Imaging multiple intermediates of single-virus membrane fusion mediated by distinct fusion proteinsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Membrane fusion plays an essential role in the entry of enveloped viruses into target cells. The merging of viral and target cell membranes is catalyzed by viral fusion proteins, which involves multiple sequential steps in the fusion process. However, the fusion mechanisms mediated by different fusion proteins involve multiple transient intermediates that have not been well characterized. Here, we report a synthetic virus platform that allows us to better understand the different fusion mechanisms driven by the diverse types fusion proteins. The platform consists of lentiviral particles coenveloped with a surface antibody,...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Kye-Il Joo, April Tai, Chi-Lin Lee, Clement Wong, Pin Wang Source Type: journals

Unbiased stereological estimation of the rat fetal pituitary volume and of the total number and volume of TSH cells after maternal dexamethasone applicationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Glucocorticoids have an inhibitory influence on proliferation activity of the pituitary cells while stimulating apoptosis. Therefore, it was hypothesized that the synthetic glucocorticoid, dexamethasone (DX), has an inhibitory influence on the number of thyroid-stimulating hormone (TSH) cells during fetal development. The effects of maternal administration of DX on stereological parameters of TSH cells, and TSH serum concentration were investigated in 21-day-old rat fetuses. On day 16 of pregnancy, the experimental dams received 1.0 mg DX/kg b.w. subcutaneously, followed by 0.5 mg DX/kg b.w./day on days 17 and 18 of gestat...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Milica Manojlovi[cacute]-Stojanoski, Nata[scaron]a Nestorovi[cacute], Nata[scaron]a Risti[cacute], Svetlana Trifunovi[cacute], Branko Filipovi[cacute], Branka [Scaron]o[scaron]i[cacute]-Jurjevi[cacute], Milka Sekuli[cacute] Source Type: journals

Electron microscopy studies of electron-beam sensitive PbTe-based nanostructuresEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PbTe nanocrystals were synthesized and then reacted with a toluene solution containing AuCl3. Depending on the reaction conditions and on the starting PbTe nanocrystals morphology, different kinds of nanostructures have been obtained: single defect-free PbTe nanocrystals with and without an external amourphous oxide shell, crystalline-Au core/amorphous PbxTeyAuz shell structure, large balloon-shaped (or mushroom-shaped) Au domain attached via its apex to the surface of the nanocrystals. Structure and composition of these different types of nanostructure have been studied by means of Cs-corrected High Resolution Transmissio...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Andrea Falqui, Giovanni Bertoni, Alessandro Genovese, Sergio Marras, Mario Malerba, Isabella R. Franchini, Liberato Manna Source Type: journals

Effect of encapsulation or grafting on release kinetics of recombinant human bone morphogenetic protein-2 from self-assembled poly(lactide-co-glycolide ethylene oxide fumarate) nanoparticlesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The objective of this work was to compare the release characteristics of Recombinant human bone morphogenetic protein-2 (rhBMP-2) encapsulated in thermally self-assembled poly(lactide ethylene oxide fumarate) (PLEOF) nanoparticles (NPs) with rhBMP-2 grafted to succinimide-terminated poly(lactide fumarate) (PLAF-NHS) or poly(lactide-co-glycolide fumarate) (PLGF-NHS) NPs. The amphiphilic PLEOF NPs had average size of 110 ± 50 nm. The hydrophobic PLAF-NHS and PLGF-NHS NPs had average size of 242 ± 67 and 195 ± 42 nm, respectively. PLEOF NPs had rhBMP-2 encapsulation efficiency ranging from 65 to 93%. Grafting efficiency of...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Angel E. Mercado, Esmaiel Jabbari Source Type: journals

Multiscale observation of biological interactions of nanocarriers: From nano to macroEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Microscopic observations have played a key role in recent advancements in nanotechnology-based biomedical sciences. In particular, multiscale observation is necessary to fully understand the nano-bio interfaces where a large amount of unprecedented phenomena have been reported. This review describes how to address the physicochemical and biological interactions of nanocarriers within the biological environments using microscopic tools. The imaging techniques are categorized based on the size scale of detection. For observation of the nanoscale biological interactions of nanocarriers, we discuss atomic force microscopy (AFM...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Su-Eon Jin, Jin Woo Bae, Seungpyo Hong Source Type: journals

Combination of atomic force microscopy and principal component analysis as a general method for direct recognition of functional and structural domains in nanonocomposite materialsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this work, we report a simple method to direct identify nanometer sized textures in composite materials by means of AFM spectroscopy, aiming at recognizing structured region to be further investigated. It consists in acquiring a set of dynamic data organized in spectroscopy maps and subsequently extracting most valuable information by means of the principal component analysis (PCA) method. This algorithm projects the information of D spectroscopy curves, each containing P data, acquired at each point of an LxC grid into a subset of LxC maps without any assumption on the sample structure, filtering out redundancies and n...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Bruno Torre, Manuele Bicego, Marco Cristiani, Vittorio Murino, Alberto Diaspro, Roberto Cingolani Source Type: journals

AFM measurement of the stiffness of layers of agarose gel patterned with polylysineEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Films of agarose gel microspotted with polylysine aqueous solution have been characterized by atomic force microscopy carried out in deionized water. Thickness and surface morphology of the layers have been checked, and the effect of polylysine impregnation on the local elasticity has been investigated. An increase in contact stiffness of the organic layer at the spotted areas has been observed, correlated with the polylysine concentration. For the considered agarose layer thickness of [sim]0.9 [mu]m in dry condition, the concentration threshold at which stiffening appears is [sim]0.1 mg/mL. Above this threshold, the stiff...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Marco Salerno, Silvia Dante, Niranjan Patra, Alberto Diaspro Source Type: journals

3D reconstruction of histological sections: Application to mammary gland tissueEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present an experimental validation of this tool using both synthetic and real data. In particular, we present the 3D reconstruction of an entire mouse mammary gland and demonstrate the integration of high-resolution molecular data. Microsc. Res. Tech., 2010. © 2010 Wiley-Liss, Inc. (Source: Microscopy Research and Technique)
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Ignacio Arganda-carreras, Rodrigo Fernández-GonzáLez, Arrate Muñoz-Barrutia, Carlos Ortiz-De-Solorzano Source Type: journals

Structural and functional consequences of poly(ethylene glycol) inclusion on DNA condensation for gene deliveryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Polycationic polymers have been used to condense therapeutic DNA into submicron particles, offering protection from shear-induced or enzymatic degradation. However, the spontaneous nature of this self-assembly process gives rise to the formation of multimolecular aggregates, resulting in significant polyplex heterogeneity. Additionally, cytotoxicity issues and serum instability have limited the in vivo efficacy of such systems. One way these issues can be addressed is through the inclusion of poly(ethylene glycol) (PEG). PEG has known steric effects that inhibit polyplex self-aggregation. A variety of PEGylated gene delive...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Peter G. Millili, Joshua A. Selekman, Kory M. Blocker, David A. Johnson, Ulhas P. Naik, Millicent O. Sullivan Source Type: journals

Cellular uptake pathway and drug release characteristics of drug-encapsulated glycol chitosan nanoparticles in live cellsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Herein, we evaluated the cellular uptake pathways of hydrophobically modified glycol chitosan (HGC) nanoparticles as nano-sized drug carriers using cellular imaging technology. The endocytic pathway of nanocarriers for intracellular drug delivery is of great interest for the design of high efficacy delivery carriers for therapeutic agents. To evaluate the cellular uptake pathways of HGC nanoparticles, HGC was chemically labeled with near infrared (NIR) fluorescence dye, Cy5.5, to visualize the nanoparticle under confocal laser scanning microscopy. The internalization pathways of HGC nanoparticles were evaluated after treat...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Sangjin Park, So Jin Lee, Hyunjin Chung, Songwook Her, Yongseok Choi, Kwangmeyung Kim, Kuiwon Choi, Ick Chan Kwon Source Type: journals

Formation and microscopic investigation of iron oxide aligned nanowires into polymeric nanocomposite filmsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We present a microscopic investigation of nanocomposite films of iron oxide ([gamma]-Fe2O3) magnetic nanowires (NWs) aligned into polymers, formed upon evaporation of solutions of acrylate polymer/magnetic nanoparticles under magnetic field (MF). The field causes the assembly of the [gamma]-Fe2O3 nanoparticles along the direction of the MF lines, resulting in magnetic NWs embedded throughout the entire volume of the polymer film. The scanning electron microscopy and the transmission electron microscopy studies show that the cylindrical-shaped NWs have [sim]15-[mu]m average length and are isotropically distributed throughou...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Despina Fragouli, Bruno Torre, Giovanni Bertoni, Raffaella Buonsanti, Roberto Cingolani, Athanassia Athanassiou Source Type: journals

Force spectroscopy as a tool to investigate the properties of supported lipid membranesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Solid supported lipid bilayers (SLB) are extensively used as a model for the investigation of cell membranes in a variety of spectroscopic and biophysical methods. It is nevertheless well known that the interaction with the solid substrate, such as mica or silicon, influences the properties of the membranes. In this article we have employed atomic force microscopy (AFM) in force spectroscopy mode (FS) to investigate the local mechanical properties of lipid membranes supported on mica and on polymer cushion. The lipid double layers were obtained by fusion of unilamellar vesicle of phospholipids. The polymer support was crea...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: C. Canale, M. Jacono, A. Diaspro, S. Dante Source Type: journals

Two and three-dimensional gene transfer from enzymatically degradable hydrogel scaffoldsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this report, we explored gene transfer to MSCs seeded on top or inside matrix metalloproteinase (MMP) degradable hydrogels that were loaded with DNA/poly(ethylene imine) (PEI) polyplexes. DNA/PEI polyplexes were encapsulated inside poly(ethylene glycol) (PEG) hydrogels crosslinked with MMP degradable peptides via Michael Addition chemistry. Gene transfer was visualized and quantified through using a vector encoding for green fluorescent protein and luciferase. We found that gene transfer to MSCs was possible for cells seeded both in two and three dimensions. The amount of luciferase expression was similar for cells seed...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Yuguo Lei, Quinn K.T. Ng, Tatiana Segura Source Type: journals

Rationalized approach to the determination of contact point in force-distance curves: Application to polymer brushes in salt solutions and in waterEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this work, we present two methods to determine the contact point in force-distance curves obtained with the atomic force microscope. These procedures are compared with the typical determination of contact point by a visual assessment of the data. One method, based on the assumption that the sample shows linear elastic behavior, provides results similar to those obtained by a visual assessment of the data, and will be suitable for determining the contact point in cases where ionic repulsion is not significant. The second method is based on a series of measurements in which the sample deformation is measured at increasing...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Kathryn A. Melzak, Susana Moreno-Flores, Kai Yu, Jayachandran Kizhakkedathu, José L. Toca-Herrera Source Type: journals

Time sequence of blood activation by nanoporous alumina: Studies on platelets and complement systemEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In the present work, the time sequence of blood activation by alumina membranes with different porosities (20 and 200 nm in diameter) was studied. The membranes were incubated with whole blood from 2 min to 4 h. Platelet adhesion and activation in addition to complement activation was monitored at different time points. Evaluation of platelet adhesion and activation was done by determining the change in platelet number and the levels of thrombospondin-1 (TSP-1) in the fluid phase. Scanning electron microscopy studies were done to further evaluate platelet adhesion and morphology. Immunocytochemical staining was used to eva...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Natalia Ferraz, Marjam Karlsson Ott, Jaan Hong Source Type: journals

FT-IR, Raman, RRS measurements and DFT calculation for doxorubicinEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Doxorubicin (DOXO) is a powerful anthracycline antibiotic used to treat many human neoplasms, including acute leukemias, lymphomas, stomach, breast and ovarian cancer, and bone tumors, yet causing cardiotoxicity at the same time. For this reason, there is a great interest in medical field to gain deep insight and knowledge of this molecule. Raman, Fourier Transform Infrared (FT-IR) absorption spectroscopy, and Resonance Raman scattering were performed for the vibrational characterization of DOXO molecule. Density function theorem (DFT) modeling of Raman and FT-IR spectra were used for the assignment of the vibrational freq...
Source: Microscopy Research and Technique - March 15, 2010 Category: Laboratory Medicine Authors: Gobind Das, Annalisa Nicastri, Maria Laura Coluccio, Francesco Gentile, Patrizio Candeloro, Gheorghe Cojoc, Carlo Liberale, Francesco De Angelis, Enzo Di Fabrizio Source Type: journals

High-mobility group box-1 protein promotes granulomatous nephritis in adenine-induced nephropathyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
otz & Ikuro Maruyama (Source: Laboratory Investigation AOP)
Source: Laboratory Investigation AOP - March 15, 2010 Category: Laboratory Medicine Authors: Yoko OyamaTeruto HashiguchiNoboru TaniguchiSalunya TancharoenTomonori UchimuraKamal K BiswasKo-ichi KawaharaTakao NitandaYoshihisa UmekitaMartin LotzIkuro Maruyama Tags: chronic inflammation granulomatous nephritis HMGB1 macrophages MCP-1 renal tubular epithelial cells 9 38 Source Type: journals

In vivo and in vitro propagation of intraductal papillary mucinous neoplasmsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
an Maitra, Ralph H Hruban & James R Eshleman (Source: Laboratory Investigation AOP)
Source: Laboratory Investigation AOP - March 15, 2010 Category: Laboratory Medicine Authors: Hirohiko KamiyamaMihoko KamiyamaSeung-Mo HongCollins A KarikariMing-Tseh LinMichael W BorgesMargaret GriffithAngela YoungAlexis Norris-KirbyConrad LubekMasamichi MizumaGeorg FeldmannChanjuan ShiHong LiangMichael G GogginsAnirban MaitraRalph H HrubanJames Tags: cell lines immunodeficient mice intraductal papillary mucinous neoplasm (IPMN) pancreatic cancer precursor lesions 18 36 Source Type: journals

Thyroid hormones induce activation of rat hepatic stellate cells through increased expression of p75 neurotrophin receptor and direct activation of RhoEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
& Ran Oren (Source: Laboratory Investigation AOP)
Source: Laboratory Investigation AOP - March 15, 2010 Category: Laboratory Medicine Authors: Isabel ZvibelDikla AtiasAdam PhillipsZamir HalpernRan Oren Tags: hepatic stellate cells liver fibrosis p75 neurotrophin receptor Rho activation thyroid hormones 9 34 Source Type: journals

Unification of the NCA and the ASCP Board of Registry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 20218088 [PubMed - in process] (Source: Clinical Laboratory Science : Journal of the American Society for Medical Technology)
Source: Clinical Laboratory Science : Journal of the American Society for Medical Technology - March 14, 2010 Category: Laboratory Medicine Authors: Morris S Tags: Clin Lab Sci Source Type: journals

Heparin-Induced thrombocytopenia (HIT): A case study.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Heparin-induced thrombocytopenia (HiT) ranges from an asymptomatic reaction to heparin with a transient mild thrombocytopenia (HIT I) to a life- and limb-threatening immunological reaction, heparin-induced thrombocytopenia with thrombosis (HITT or HIT II). HITT can occur in patients with any heparin exposure and must be recognized and and symptomatic and/or fatal thrombosis. HIT will be discussed using a case study approach. PMID: 20218089 [PubMed - in process] (Source: Clinical Laboratory Science : Journal of the American Society for Medical Technology)
Source: Clinical Laboratory Science : Journal of the American Society for Medical Technology - March 14, 2010 Category: Laboratory Medicine Authors: Newkirk CE Tags: Clin Lab Sci Source Type: journals

CLS to higher education administrator: the right navigational skills.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CONCLUSION: The participants in this study confirmed that by possessing a terminal degree and a defined skill set, they were able to obtain a higher education administrator position. PMID: 20218090 [PubMed - in process] (Source: Clinical Laboratory Science : Journal of the American Society for Medical Technology)
Source: Clinical Laboratory Science : Journal of the American Society for Medical Technology - March 14, 2010 Category: Laboratory Medicine Authors: Campbell S, LaCost BY Tags: Clin Lab Sci Source Type: journals