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This page shows you the most recent publications within this specialty of the MedWorm directory.

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Endothelin-1, big endothelin-1, and nitric oxide in patients with chronic renal disease and hypertensionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The complex pathogenesis of chronic renal disease (CRD) depends on endothelin (ET) axis (ETs and ET receptors) and nitric oxide (NO) because of their vasoactive effects and their role in general modulation of vascular homeostasis. Various renal cells synthesize ETs and NO that play a significant role in renal hemodynamics as well as in water and salt excretion via urine. ET-1 is a strong vasoconstrictor. Besides its vasoactive effects, ET-1 modulates mitosis and apoptosis in a cell type-dependent manner, and may play an important role in CRD pathogenesis. The aims of this study were to emphasize the role and interactions o...
Source: Journal of Clinical Laboratory Analysis - November 20, 2009 Category: Laboratory Medicine Authors: Ivanka Mikuli[cacute], József Petrik, Kre[scaron]imir Gale[scaron]i[cacute], [Zcaron]eljko Romi[cacute], Ivana [Ccaron]epelak, Monika Zeljko-Tomi[cacute] Tags: Original Articles Source Type: journals

Meet the Virtual Dermatopathology Lab, Doing Global Business From BostonEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Second opinion dermpath business combines digital pathology, glass slides, and the Internet. Telepathology, Federal Express, and internet technologies are the cornerstones of a flourishing second opinion business by dermatopathologists in Boston, Massachusetts. In just a few years, the practice has built a national and international clientele. This confirms that there is already overseas demand for access [...] (Source: Dark Daily)
Source: Dark Daily - November 20, 2009 Category: Laboratory Medicine Authors: jude Tags: Digital Pathology Laboratory Management and Operations Laboratory News Laboratory Pathology Artur Zembowicz MD PhD Dark Daily Digital imaging dark report news dermatology dermatology group Dermatopathology DermatopathologyConsultations Source Type: info

ESEM imaging of dynamic biological processes: the closure of stomatal poresEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Historically, electron microscopy of dynamic biological processes has been impossible to achieve in real time because conventional electron microscopy requires specimen fixation, dehydration and metallic coating. The advent of the environmental scanning electron microscope removes these restrictions, allowing fully hydrated samples to be imaged in their native state. We explore the possibility of secondary electron imaging of biological systems undergoing natural morphological changes in the microscope chamber and present a proof of principle study on the closure of stomatal pores in Tradescantia andersonia leaf tissue. An...
Source: Journal of Microscopy - November 20, 2009 Category: Laboratory Medicine Authors: J.E. McGREGOR, A.M. DONALD Source Type: journals

Recrystallization of plane strain compressed Al–1 wt.% Mn alloy single crystals of typical unstable orientationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A systematic study of crystal lattice reorientation in early stages of recrystallization has been carried out to correlate the orientations of recrystallization nuclei with the deformation microtexture and with slip systems. Microstructure and texture of Al[ndash]1 wt.% Mn single crystals of unstable initial orientations of {112}〈111〉, {100}〈001〉 and {001}〈110〉 have been examined by high-resolution field-emission gun scanning electron microscope local orientation measurements. All single crystals were channel-die deformed at room temperature and then annealed for a short time. It w...
Source: Journal of Microscopy - November 19, 2009 Category: Laboratory Medicine Authors: M. BIJAK, H. PAUL, J.H. DRIVER Source Type: journals

TEM studies of plasma nitrided austenitic stainless steelEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cross-sectional transmission electron microscopy and X-ray phase analysis were used to study the structure of a layer formed during nitriding the AISI 316L stainless steel at temperature 440°C. It was found that the applied treatment led to the formation of 6-[mu]m-thick layer of the S-phase. There is no evidence of CrN precipitation. The X-ray diffraction experiments proved that the occurred austenite lattice expansion [ndash] due to nitrogen atoms [ndash] depended on the crystallographic direction. The cross-sectional transmission electron microscopy studies showed that the layer consisted of a single cubic phase that c...
Source: Journal of Microscopy - November 19, 2009 Category: Laboratory Medicine Authors: D. STRÓŻ, M. PSODA Source Type: journals

Depth measurements of etch-pits in GaN with shape reconstruction from SEM imagesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The method, which allows shape reconstruction by reading the intensity from the scanning electron microscopy image, is presented and discussed in details. The method is applied to read the morphology of etch-pits, which were formed on the GaN surface by etching in molten KOH[ndash]NaOH eutectic mixture to delineate dislocations. The etch-pit depth distributions are obtained and used to determine densities of pits related to screw, mixed or edge-type dislocations. The results are compared with atomic force microscopy. (Source: Journal of Microscopy)
Source: Journal of Microscopy - November 19, 2009 Category: Laboratory Medicine Authors: M. WZOREK, A. CZERWINSKI, J. RATAJCZAK, A. LUI, E. IACOB, J. KĄTCKI Source Type: journals

TEM studies of melt-spun alloys with liquid miscibility gapEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The microstructures of the Fe[ndash]Cu-based alloys melt-spun from various temperatures are presented. Two compositions, with different Fe and Cu content, revealing liquid miscibility gap, were studied. Nonuniform, large-elongated areas were observed for lower melt-spinning temperatures, indicating liquid/liquid phase separation in the crucible before cooling. An increase of the melt ejection temperature, followed by rapid cooling, brought about precipitation process within homogeneous melt. Studies of the system with higher Fe content proved amorphous nature of the matrix and crystalline structure of the primary formed Cu...
Source: Journal of Microscopy - November 19, 2009 Category: Laboratory Medicine Authors: T. KOZIEŁ, Z. KĘDZIERSKI, A. ZIELIŃSKA-LIPIEC, J. LATUCH, G. CIEŚLAK Source Type: journals

Electron detection in the intermediate chamber of the variable pressure SEMEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Two concepts of the secondary electron detection inside the intermediate chamber of the variable pressure scanning electron microscopy have been discussed. One of them, the two-stage secondary electron detector has been the subject of previous publications. The other one, the intermediate secondary electron detector is an improved solution in respect of its complexity, vacuum demands and dimensions. Both detector systems apply some kinds of scintillators, so they present advantages specific for scintillator detectors but extended to a wide range of gas pressures, from high vacuum to pressures exceeding 10 mbar. The detecto...
Source: Journal of Microscopy - November 19, 2009 Category: Laboratory Medicine Authors: W. SLÓWKO, M. KRYSZTOF Source Type: journals

Dependence of cathodoluminescence on layer resistance applied for measurement of thin-layer sheet resistanceEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The dependence of spatially and spectrally resolved cathodoluminescence in a scanning electron microscope on resistances in semiconductor structures, especially on the layer resistance, is reported. This previously unstudied dependence is utilized for thin-layer sheet-resistance measurement. The method is illustrated by an assessment of lateral confinements in semiconductor-laser heterostructures. (Source: Journal of Microscopy)
Source: Journal of Microscopy - November 19, 2009 Category: Laboratory Medicine Authors: A. CZERWINSKI, M. PLUSKA, J. RATAJCZAK, A. SZERLING, J. KąTCKI Source Type: journals

Shear banding phenomenon in a Cu–8 at.% Al alloy analysed by orientation imaging microscopyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The microstructure and texture of Cu[ndash]8 at.% Al alloy single crystal with (112)[11] orientation plane strain compressed at 77 K were characterized by scanning electron microscope and transmission electron microscope orientation mappings in order to investigate the influence of twins and shear bands on slip propagation across a structure of twin-matrix layers and the resulting texture evolution. It was shown that the strong, initial texture changes are due to deformation twinning at low deformations. At larger deformations, twin-matrix bending within some narrow areas led to the formation of kink-type bands, which beca...
Source: Journal of Microscopy - November 19, 2009 Category: Laboratory Medicine Authors: H. PAUL, A. MORAWIEC, M. DARRIEULAT, E. BOUZY Source Type: journals

Identification of electron beam vibration sources by separation of magnetic distortion from electric distortion on scanning electron microscope imagingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Different types of distortions in scanning electron microscopy require different methods of their elimination, and therefore influence of these types on particular elements of the SEM system should be known. The proposed method allows for separation of the direct influence of the magnetic field on the electron beam in the SEM chamber from its influence in the SEM column and from the distortions generated in the SEM scanning block. For this purpose, a series of distorted images is registered for several working distances (between the final aperture of the electron column and the specimen) and for several energies of the ele...
Source: Journal of Microscopy - November 19, 2009 Category: Laboratory Medicine Authors: M. PLUSKA, A. CZERWINSKI, J. RATAJCZAK, J. KĄTCKI, Ł. OSKWAREK, R. RAK Source Type: journals

TEM characterization of MBE grown CdTe/ZnTe axial nanowiresEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
CdTe/ZnTe axial nanowires were successfully fabricated by molecular beam epitaxy with the use of Au nano-catalysts and vapour[ndash]liquid[ndash]solid growth mechanism. Nanowires had zinc-blende structure with numerous stacking faults in the bottom ZnTe part and near perfect crystalline structure in the top CdTe part. Energy dispersive X-ray spectroscopy (EDXS) and lattice fringe spacing analysis revealed nonabrupt nature of hetero[minus]interface, whose width was estimated to be 50[ndash]70 nm for the nanowires having a diameter in the range from 40 to 50 nm. (Source: Journal of Microscopy)
Source: Journal of Microscopy - November 19, 2009 Category: Laboratory Medicine Authors: P. DŁUZEWSKI, E. JANIK, S. KRET, W. ZALESZCZYK, D. TANG, G. KARCZEWSKI, T. WOJTOWICZ Source Type: journals

Microstructural changes during creep of CMSX-4 single crystal Ni base superalloy at 750°CEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
TEM studies of creep tested CMSX-4 nickel-base single crystal superalloy were performed to analyse a microstructure evolution during creep at temperature 750°C, and uniaxial tensile stress of 675 MPa. Microstructural analyses were focused mainly on examination of dislocation configurations during primary and secondary creep stages of high temperature deformation. At such low temperature and high stress creep deformation proceed by cutting of [gamma]' particles by dislocations. It was found that primary creep is initiated by movement of dislocations with Burgers vector a/2 in the [gamma] phase. The second type of dislocati...
Source: Journal of Microscopy - November 19, 2009 Category: Laboratory Medicine Authors: B. T. DUBIEL, A. CZYRSKA-FILEMONOWICZ Source Type: journals

IgM, IgG, and IgA anti-DNA topoisomerase I antibodies in systemic sclerosisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This report confirms that different immunoglobulin classes with anti-topo I activity may occur in SSc. IgG was the predominant serological feature with both, the recombinant and native antigens. The study also demonstrates the association between high levels of these autoantibodies and some clinical manifestations of SSc. J. Clin. Lab. Anal. 23:408-416, 2009. © 2009 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Erasmo Martínez-Cordero, Alejandro Padilla Trejo, Diana E. Aguilar León Tags: Original Articles Source Type: journals

Prevalence characterization of extended-spectrum [beta]-lactamases among Escherichia coli isolates collected in ZhengzhouEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We describe a complex ESBL epidemiology. The study revealed a high rate of ESBL-producing E. coli isolates. TEM and CTX-M enzymes dominated in ESBL-positive E. coli isolates in Zhengzhou, China. J. Clin. Lab. Anal. 23:404-407, 2009. © 2009 Wiley-Liss, Inc. (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Zhijian Zhang, Xiaobing Guo, Qinxian Zhang Tags: Original Articles Source Type: journals

New method for calcium on the ADVIA analyzer is free from interference of gadolinium-type contrast agentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recently, Siemens Diagnostics released a new calcium assay (CA_2) based on complex formation of calcium with Arsenazo III dye for use on the three automated, random access ADVIA Chemistry analyzers (1650, 2400, and 1200). We evaluated this method for analytical performance as well as potential interference from gadolinium-containing magnetic contrast agents. With Siemens Chemistry serum and urine controls, 2-levels each, the imprecision for the new method was (n=40 each): within-run and total CV of (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Pradip Datta, Amitava Dasgupta Tags: Original Articles Source Type: journals

Rapid liquid chromatography/tandem mass spectrometer (LCMS) method for clozapine and its metabolite N-desmethyl Clozapine (Norclozapine) in human serumEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study describes a simple liquid chromatography/tandem mass spectrometer (LCMS) method in human serum. The ion transitions monitored were m/z 327, 270, 296 for Clozapine, m/z 313, 192, 227 for Norclozapine and m/z 328, 271 for Loxapine. The assay is linear (25-1000 ng/ml) and showed a good correlation (r=0.98) within the analytical range of 79-1210 ng/ml in human serum. This assay is highly specific and sensitive for the simultaneous measurements of Clozapine and Norclozapine. The simplification of this assay makes it ideal for high throughput analyses of the patient samples in a routine clinical laboratory staffed wit...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: L.V. Rao, M.L. Snyder, G.M. Vallaro Tags: Original Articles Source Type: journals

CXCL12 rs1801157 polymorphism in patients with breast cancer, hodgkin's lymphoma, and non-hodgkin's lymphomaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chemokines and their receptors regulate the trafficking of immune cells during their development, inflammation, and tissue repair. The single-nucleotide polymorphism (SNP) rs1801157 (previously known as CXCL12-A/ stromal cell-derived factor-1 (SDF1)-3[prime]A) in CXCL12/SDF1 gene was assessed in breast cancer, Hodgkin's lymphoma (HL), and non-Hodgkin's lymphoma (NHL), since the chemokine CXCL12, previously known as SDF1, and its receptor CXCR4 regulate leukocyte trafficking and many essential biological processes, including tumor growth, angiogenesis, and metastasis of different types of tumors. Genotyping was performed by...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Karen Brajão de Oliveira, Julie Massayo Maeda Oda, Julio Cesar Voltarelli, Thiago Franco Nasser, Mario Augusto Ono, Thiago Cezar Fujita, Tiemi Matsuo, Maria Angelica Ehara Watanabe Tags: Original Articles Source Type: journals

Gene diagnosis for nine Chinese patients with DMD/BMD by multiplex ligation-dependent probe amplification and prenatal diagnosis for one of themEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aims to perform gene diagnosis for nine patients with Duchenne/Becker muscular dystrophy (DMD/BMD) and their parents with multiplex ligation-dependent probe amplification (MLPA), and to carry out prenatal gene diagnosis for one of them. Genomic DNA of the peripheral blood and fetal amniotic fluid cell was extracted from the pedigrees' members with DMD/BMD. Gene diagnosis was performed for theses pedigrees' members using a SALSA KIT. Short tandem repeats (STR) genotyping and X-linkage analysis were performed for the pedigree members of the fetus, which was used in the prenatal diagnosis. MLPA analysis results sho...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Yupeng Wu, Gengxin Yin, Keqin Fu, De Wu, Qian Zhai, Huarong Du, Zhongjun Huang, Yuhua Niu Tags: Original Articles Source Type: journals

Increased soluble serum markers caspase-cleaved cytokeratin-18, histones, and ST2 indicate apoptotic turnover and chronic immune response in COPDEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we hypothesized that systemic markers of apoptosis are altered in patients with mild-to-severe COPD.Materials and Methods: A total number of 64 patients and controls were enrolled in the study. Lung function parameters of all groups (nonsmoker, healthy smoker, COPD GOLD I&II, COPD GOLD III&IV) were evaluated at the time of inclusion. Enzyme-linked immunosorbent assays were used to quantify protein levels in serum samples.Results: Serum contents of apoptotic end-products caspase-cleaved cytokeratin-18 and histone-associated-DNA-fragments were increased in patients with COPD, whereas anti-inflammatory soluble ...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Stefan Hacker, Christopher Lambers, Andreas Pollreisz, Konrad Hoetzenecker, Michael Lichtenauer, Andreas Mangold, Tina Niederpold, Andreas Hacker, György Lang, Martin Dworschak, Thomas Vukovich, Christopher Gerner, Walter Klepetko, Hendrik Jan Ankersmit Tags: Original Articles Source Type: journals

AML1 amplification and 17q25 deletion in a case of childhood acute lymphoblastic leukemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We report a case of childhood acute lymphoblastic leukemia (ALL) with both acute myeloid leukemia 1 (AML1) amplification and 17q25 deletion. AML1 gene is located on 21q22 and encodes a transcription factor. AML1 amplification is a common finding in childhood ALL, and itis observed as an increase in gene copy number by the FISH analysis. The mechanism of AML1 amplification is not associated with AML1 gene mutations. The 17q25 is a gene-rich chromosomal location and distinct abnormalities of this region have been observed in previous cases of different kinds of leukemia. Deletion of the 17q25 region has been reported in two ...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Tuna Gulten, Tahsin Yakut, Mutlu Karkucak, Birol Baytan, Adalet Meral Gune[scedil] Tags: Original Articles Source Type: journals

A novel genetic variant of BMP2K contributes to high myopiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Loss of eye growth regulation may cause myopia, because modulation of optic globe size is essential for the generation of normal optic power. Evidence has implied variations of BMP2 gene expression mediate ocular development and retinal tissue remodeling. Given BMP2 as a potential regulator involved in myopia development, we investigate whether gene BMP2-inducible kinase (BMP2K, BIKe), whose expression is up-regulated during BMP2-induced osteoblast differentiation, contributes to susceptibility of high myopia. Participants grouped into high myopia had a spherical equivalent greater than -6.00 D, compared with a control gro...
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Hsin-Ping Liu, Ying-Ju Lin, Wei-Yong Lin, Lei Wan, Jim Jinn-Chyuan Sheu, Hui-Ju Lin, Yuhsin Tsai, Chang-Hai Tsai, Fuu-Jen Tsai Tags: Original Articles Source Type: journals

Human epididymis protein 4 is a biomarker for transitional cell carcinoma in the urinary systemEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Objective: To investigate human epididymis protein 4 (HE4) levels in transitional cell carcinoma (TCC) of the urinary system and its relationship with clinicopathological features.Methods: 102 patients with TCC, 60 with benign urinary diseases, and 60 healthy controls were included in this study. The HE4 levels were used to analyze different clinicopathologic characteristics and changes between pre- and postsurgical operation.Results: The HE4 level was significantly increased in patients with TCC compared to patients with benign urinary diseases patients (P (Source: Journal of Clinical Laboratory Analysis)
Source: Journal of Clinical Laboratory Analysis - November 19, 2009 Category: Laboratory Medicine Authors: Zhu Xi, Ma LinLin, Tian Ye Tags: Original Articles Source Type: journals

Pathology Testing Prices as a Commodity: Australia’s Health System Stands at a CrossroadsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Increased Test Volumes in Recent Years Trigger an Important Review of Coverage and Funding for Pathology Testing Services DATELINE: MELBOURNE, AUSTRALIA—Pathology testing is under the gun in Australia. Fast-rising utilization of pathology testing over the past decade has caught the attention of health system policy makers. They are concerned about the funding and clinical service implications [...] (Source: Dark Daily)
Source: Dark Daily - November 18, 2009 Category: Laboratory Medicine Authors: Justin Clark Tags: Laboratory Management and Operations Laboratory Pathology AACB Australasian Association of Clinical Biochemists Australasian Meetings clinical laboratory genetic testing Graeme Suthers MD Jane Hall Ken Worth laboratory pricing Lean Source Type: info

JALA Information for authorsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of the Association for Laboratory Automation)
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Tags: Meetings and Events Source Type: journals

World NewsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The Exoscan portable FTIR spectrometer system now offers increased sampling and usability capabilities that extend its capability to an array of applications for lab and/or field use. New sampling interfaces for the Exoscan system include internal reflectance, grazing angle reflectance, diffuse reflectance, and specular reflectance. With these enhancements, Exoscan can accommodate liquids, powders, pastes, and reflective and nonreflective solids. (Source: Journal of the Association for Laboratory Automation)
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Tags: World News Source Type: journals

Please Do Pay Attention to the Man Behind the CurtainEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
“…ALA is an impressive best practices model from which other associations and professional societies learn.” (Source: Journal of the Association for Laboratory Automation)
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Erik Rubin Tags: From the President Source Type: journals

Changes, Challenges, and ChoicesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
“ALA and JALA have become the intersection at which the vitality of like minds not only connects, but combusts and creates.” (Source: Journal of the Association for Laboratory Automation)
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Dean Ho Tags: From the Editor-in-Chief Source Type: journals

Editorial BoardEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of the Association for Laboratory Automation)
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Tags: Frontmatter Source Type: journals

Table of ContentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of the Association for Laboratory Automation)
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Tags: Frontmatter Source Type: journals

Subject IndexEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of the Association for Laboratory Automation)
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Tags: Meetings and Events Source Type: journals

Author IndexEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: Journal of the Association for Laboratory Automation)
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Tags: Meetings and Events Source Type: journals

Meetings and EventsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
December 5–8 2009 ASH Annual Meeting: New Orleans, LA (Source: Journal of the Association for Laboratory Automation)
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Tags: Meetings and Events Source Type: journals

A Microfluidic Cartridge System for Multiplexed Clinical AnalysisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cartridge-based microfluidics is a promising technology for clinical diagnostics. By miniaturizing the fluid-handling processes required for genomic and proteomic analyses, reagent and specimen volume is minimized along with the size of the system. We demonstrate an automated microfluidic system capable of performing six multiplexed genomic and proteomic analyses simultaneously, by means of an integrated electrochemical sensor and embedded controls. (Source: Journal of the Association for Laboratory Automation)
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Walson Lawi, Chris Wiita, Steven T. Snyder, Fang Wei, David Wong, Pak Kin Wong, Joseph C. Liao, David Haake, Vincent Gau Tags: Technology Review Source Type: journals

Iridium Oxide Nanomonitors for Real-time Health Monitoring SystemsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An innovative lab-on-a-chip device based on iridium oxide (IrOx) nanowires was designed, fabricated, and developed for real-time “point-of-care” diagnostics. The turnaround time in detection and the need for expensive equipment for analysis have considverably limited rapid and “point-of-care” diagnosis. This research demonstrates the potential of IrOx nanowires toward early disease diagnosis by detecting proteins that are disease markers. The device designed is based on electrical detection of protein biomarkers wherein a single capture immunoassay is built onto a vertically aligned IrOx nanowire platform. Detectio...
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Vinu L. Venkatraman, Fengyan Zhang, David Evans, Bruce Ulrich, Shalini Prasad Tags: Original Reports Source Type: journals

Analysis of Retina and Erythrocyte Glycerophospholipid Alterations in a Rat Model of Type 1 DiabetesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An automated tandem mass spectrometry–based analysis using precursor ion and neutral loss scans in a triple quadrupole (QqQ) mass spectrometer has been used to identify and quantify changes in the abundances of glycerophospholipids extracted from retina and erythrocytes in a rat streptozotocin model of type 1 diabetes, 6 and 36 weeks after the induction of diabetes, compared with age-matched nondiabetic controls. The utility of an “internal standard” method compared with an “internal standard free” method for quantification of differences in the abundances of specific lipid ions was evaluated in both retina and e...
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Todd A. Lydic, Rebecca Renis, Julia V. Busik, Gavin E. Reid Tags: Original Reports Source Type: journals

Pathway Reporter Assays Reveal Small Molecule Mechanisms of ActionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cell-based, phenotypic screening of small molecules often identifies compounds with provocative biological properties. However, determining the cellular target(s) and/or mechanism of action (MoA) of lead compounds remains an extremely challenging and time-consuming exercise. To provide insights into a compound's cellular action and greatly reduce the time required for MoA determination, we have developed a screening platform consisting of an extensive series of reporter gene assays (RGAs). A collection of >11,000 compounds of known MoA (e.g., World Drug Index entries) were screened against the entire panel. The output prov...
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Frederick J. King, Douglas W. Selinger, Felipa A. Mapa, Jeff Janes, Hua Wu, Timothy R. Smith, Qing-Yin Wang, Pornwaratt Niyomrattanakitand, Daniel G. Sipes, Achim Brinker, Jeffrey A. Porter, Vic E. Myer Tags: Original Reports Source Type: journals

High-Pressure/High-Temperature Microreactors for Nanostructure SynthesisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe continuous synthesis of nanostructures in microfluidic systems consisting of multiple sub–millimeter-sized channels in which fluid flows continuously and chemical reactions take place. The small reaction volumes combined with the high heat and mass transfer rates enable reactions to be performed under more controlled conditions with higher yields than can typically be achieved with conventional reactors. Moreover, manipulation of reaction parameters, while the reaction proceeds, allows optimization of synthesis conditions. The ability to work at elevated temperatures and pressures while confining potentially ...
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Samuel Marre, Jinyoung Baek, Jongnam Park, Moungi G. Bawendi, Klavs F. Jensen Tags: Original Reports Source Type: journals

Automation of the SomaLogic Proteomics Assay: A Platform for Biomarker DiscoveryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
At SomaLogic, we have embarked on an ambitious program of clinical studies using a novel aptamer-based proteomics technology to discover biomarkers and develop new tools to diagnose, understand, and treat human disease. As part of this program, we designed and implemented an automated assay for its highly multiplexed proteomics discovery platform. The performance of the automated assay was validated in a study that compared the automated assay to the specifications of an established manual method. Results showed that the automated method performed to the required specifications, and that the automation system improved the ...
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Tracy R. Keeney, Christopher Bock, Larry Gold, Stephan Kraemer, Bridget Lollo, Malti Nikrad, Martin Stanton, Alex Stewart, Jonathan D. Vaught, Jeffrey J. Walker Tags: Original Reports Source Type: journals

A Method for Identifying Small-Molecule Aggregators Using Photonic Crystal Biosensor MicroplatesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Small molecules identified through high-throughput screens are an essential element in pharmaceutical discovery programs. It is now recognized that a substantial fraction of small molecules exhibit aggregating behavior leading to false positive results in many screening assays, typically due to nonspecific attachment to target proteins. Therefore, the ability to efficiently identify compounds within a screening library that aggregate can streamline the screening process by eliminating unsuitable molecules from further consideration. In this work, we show that photonic crystal (PC) optical biosensor microplate technology ca...
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Leo L. Chan, Erich A. Lidstone, Kristin E. Finch, James T. Heeres, Paul J. Hergenrother, Brian T. Cunningham Tags: Original Reports Source Type: journals

Backscattering Interferometry for Low Sample Consumption Molecular Interaction ScreeningEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Backscattering interferometry (BSI), which uses a simple optical train comprising a He–Ne laser, a microfluidic channel, and a position sensor, has now enabled the measurement of both tethered and free-solution, label-free, molecular interactions within just nanoliters of sample. The simple macro-to-micro interface allows for a highly efficient assay work flow, which has been used to interrogate molecular binding interactions between proteins, ions and protein, and small molecules and proteins, with a high dynamic range of dissociation constants (KD) and unmatched sensitivity. With this technique, the equilibrium KD for ...
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Amanda Kussrow, Carolyn S. Enders, Ereny F. Morcos, Darryl J. Bornhop Tags: Original Reports Source Type: journals

Perspectives on Utilizing Unique Features of Microfluidics Technology for Particle and Cell SortingEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this report, we use recent examples from our work to illustrate the potential of microfluidic cell- and particle-sorting devices. We demonstrate the potential of chip-based high-gradient magnetophoresis that enable high-purity separation through reversible trapping of target particles paired with high-stringency washing with minimal loss. We also describe our work in the development of devices that perform simultaneous multitarget sorting, either through precise control of magnetic and fluidic forces or through the integration of multiple actuation forces into a single monolithic device. We believe that such devices may...
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Jonathan D. Adams, H. Tom Soh Tags: Original Reports Source Type: journals

Automation Highlights from the LiteratureEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Automated semipreparative LC/MS systems are now well established commercially and commonly used for purification of early stage drug discovery compounds. A number of vendors have instruments on the market that are capable of reliably purifying compounds with good water/acetonitrile solubility. However, these systems often fail when the sample has poor solubility, extreme polarity, and/or poor ionization. Even in cases when substantial optimization has been done before purification, a certain percent of failures to recover the desired product is unavoidable. In the past, when most of the samples run on LC/MS semipreparative...
Source: Journal of the Association for Laboratory Automation - November 16, 2009 Category: Laboratory Medicine Authors: Kerstin Thurow, Hilmar Weinmann Tags: Literature Highlights Source Type: journals

GSTP1*B allele increases the risk for asthma in childrenEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 47 (11): 1451-1453 (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - November 16, 2009 Category: Laboratory Medicine Source Type: journals

Quick and easy colorimetric test for calibration control of liquid handling workstationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 47 (11): 1447-1449 (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - November 16, 2009 Category: Laboratory Medicine Source Type: journals

Hypoxia related growth factors and p53 in preoperative sera from patients with colorectal cancer – evaluation of the prognostic significance of these agentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 47 (11): 1439-1445 Abstract Background: Insulin-like growth factor-I (IGF-I) and vascular endothelial growth factor (VEGF) belong to a group of hypoxia related proteins. IGF-I induces expression of VEGF and decomposes wild type p53 in cancer cell lines. The goal of our study was to evaluate serum IGF-I, VEGF and p53 with respect to overall and disease free survival of patients with colorectal cancer (CRC) patients compared with healthy volunteers. Methods: Preoperative blood samples from 125 patients with CRC and 16 healthy volunteers were examined using ELISA for serum IGF-I, p53...
Source: Clinical Chemistry and Laboratory Medicine - November 16, 2009 Category: Laboratory Medicine Source Type: journals

Biochemical role of intravaginal sildenafil citrate as a novel antiabortive agent in unexplained recurrent spontaneous miscarriage: first clinical study of four case reports from EgyptEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: We conclude that intravaginal sildenafil citrate tablets used as suppositories might be a novel, interesting, safe antiabortive option in the treatment of threatened miscarriage in patients with a history of URSM. We are the first to report this novel clinical application in four case reports in Egypt. Clin Chem Lab Med 2009;47:1433–8. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - November 16, 2009 Category: Laboratory Medicine Source Type: journals

Potential of the Sebia Capillarys® neonat fast automated system for neonatal screening of sickle cell diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Sebia Capillarys® neonat fast automated system is a reliable tool for haemoglobinopathy neonatal screening. Clin Chem Lab Med 2009;47:1423–32. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - November 16, 2009 Category: Laboratory Medicine Source Type: journals

A new turbidimetric method for assaying serum C-reactive protein based on phosphocholine interactionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The functional CRP assay allowed measurement of CRP in serum and plasma in the range of 7 mg/L–400 mg/L. The assay is particularly suited in conditions where resources are restricted. Since the assay is species independent, the described functional CRP assay could be used for veterinary purposes as well. Clin Chem Lab Med 2009;47:1417–22. (Source: Clinical Chemistry and Laboratory Medicine)
Source: Clinical Chemistry and Laboratory Medicine - November 16, 2009 Category: Laboratory Medicine Source Type: journals

Potential utility of the new Sysmex XE 5000 red blood cell extended parameters in the study of disorders of iron metabolismEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clinical Chemistry and Laboratory Medicine 47 (11): 1411-1416 Abstract Background: New erythrocyte parameters are reported by the Sysmex XE 5000 analyzer. This instrument measures the hemoglobin (Hb) content of individual red cells, calculates the percentage of hypochromic red cells (%Hypo He) and the percentage of hyperchromic red cells (%Hyper He) and quantifies the proportion of marginally sized erythrocytes (%Micro R and %Macro R). The goals of the study were to establish the reference range for erythrocyte extended parameters, their value in different types of anemia and to investigate their reliability in the study o...
Source: Clinical Chemistry and Laboratory Medicine - November 16, 2009 Category: Laboratory Medicine Source Type: journals