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This page shows you the most recent publications within this specialty of the MedWorm directory. This is page number 19.

Infantile Acne Treated with Oral Isotretinoin
Abstract In contrast to adolescent acne, infantile acne (IA) is a rare condition with only a limited body of available literature. In this descriptive, retrospective study, we reviewed six cases from 2002 to 2010 treated with oral isotretinoin. The average age of onset was 6.16 months (range 0–21 mos). Consistent with the previous, limited literature, we found predominantly boys are affected, a predilection for the cheeks, and a polymorphic inflammatory morphology. Two patients had a family history of acne. All cases were successfully and safely treated with oral isotretinoin. The suggested treatment of childhood acne i...
Source: Pediatric Dermatology - December 28, 2012 Category: Dermatology Authors: Iben Marie Miller, Begoña Echeverría, Antonio Torrelo, Gregor Borut Ernst Jemec Tags: Clinical And Laboratory Observation Source Type: research

Identification of Human Corneal Epithelial Stem Cells on the Basis of High ABCG2 Expression Combined With a LargeN/C Ratio
ConclusionsWe demonstrate the importance of combining ABCG2 expression with N/C ratio for identification and quantification of human CESCs. This method will have application in evaluating the role of niche cells/factors in the maintenance of stemness. Microsc. Res. Tech., 2012. © 2012 Wiley Periodicals, Inc.
Source: Microscopy Research and Technique - December 28, 2012 Category: Laboratory Medicine Authors: Chidambaranathan Gowri Priya, Tilak Prasad, Namperumalsamy Venkatesh Prajna, Veerappan Muthukkaruppan Tags: Research Article Source Type: research

Microscopic, Molecular, and Biochemical Investigations to Characterize a Benthic Cyanoprokaryote Leptolyngbya StrainFrom Egypt
ABSTRACT Microscopic, molecular, and biochemical investigations were conducted to describe a benthic mat‐forming Leptolyngbya isolate collected from wastewater canal in Helwan area, Egypt. Microscopic examination revealed that the isolate was filamentous, nonheterocystous, with obvious granular surface ornamentation. Electron microscopy was used to reveal the isolate's ultrastructure. Cross walls were thick with uneven deposition. Thylakoids were convoluted and irregularly distributed. Granular content differed from one cell to the other probably due to their physiological stages/position within the filaments and/or thei...
Source: Microscopy Research and Technique - December 28, 2012 Category: Laboratory Medicine Authors: Nermin Adel El Semary Tags: Research Article Source Type: research

Novel Use of Scanning Electron Microscopy for Detection of Iron‐Induced Morphological Changes in Human Blood
ABSTRACT Fibrinogen is key to the maintenance of hemostasis and is an acute phase protein that is part of the coagulation cascade of proteins. It plays a fundamental role in inflammation, particularly as indicator for a proinflammatory state and is a prominent marker for developing vascular inflammatory diseases. The ultrastructure of fibrin nets can be studied using scanning electron microscopy (SEM) with the addition of thrombin to plasma. In inflammatory conditions such as thromboembolic ischemic stroke and diabetes, the fibrin networks are changed to from dense matted fibrin deposits (DMDs) instead of typical netlike a...
Source: Microscopy Research and Technique - December 28, 2012 Category: Laboratory Medicine Authors: Etheresia Pretorius, Natasha Vermeulen, Janette Bester, Boguslaw lipinski Tags: Research Article Source Type: research

Spider silk reduces insect herbivory.
Abstract The role of predators in food webs extends beyond their ability to kill and consume prey. Such trait-mediated effects occur when signals of the predator influence the behaviour of other animals. Because all spiders are silk-producing carnivores, we hypothesized that silk alone would signal other arthropods and enhance non-lethal effects of spiders. We quantified the herbivory inflicted by two beetle species on green bean plants (Phaseolus vulgaris) in the presence of silkworm silk and spider silk along with no silk controls. Single leaflets were treated and enclosed with herbivores in the laboratory and fi...
Source: Biology Letters - December 27, 2012 Category: Biology Authors: Rypstra AL, Buddle CM Tags: Biol Lett Source Type: research

Mutation accumulation and fitness in mutator subpopulations of Escherichia coli.
Abstract Bacterial populations in clinical and laboratory settings contain a significant proportion of mutants with elevated mutation rates (mutators). Mutators have a particular advantage when multiple beneficial mutations are needed for fitness, as in antibiotic resistance. Nevertheless, high mutation rates potentially lead to increasing numbers of deleterious mutations and subsequently to the decreased fitness of mutators. To test how fitness changed with mutation accumulation, genome sequencing and fitness assays of nine Escherichia coli mutY mutators were undertaken in an evolving chemostat population at three...
Source: Biology Letters - December 27, 2012 Category: Biology Authors: Maharjan RP, Liu B, Li Y, Reeves PR, Wang L, Ferenci T Tags: Biol Lett Source Type: research

Rat aversion to isoflurane versus carbon dioxide.
Abstract Some experts suggest that sedation of laboratory rodents with isoflurane before euthanasia with carbon dioxide (CO(2)) is a humane alternative to euthanasia with CO(2) alone, but little research has compared aversion with these agents. Albino rats were tested in a light-dark box where they had the choice between remaining in a dark compartment filling with isoflurane or CO(2), or escaping to a lit compartment. Experiment 1 validated the procedure by confirming that rats responded to agent and light intensity. In experiment 2, 9/16 and 0/16 rats remained in the dark compartment until recumbent when initiall...
Source: Biology Letters - December 27, 2012 Category: Biology Authors: Wong D, Makowska IJ, Weary DM Tags: Biol Lett Source Type: research

Bis(acridine-9-carboxylate)-nitro-europium(III) dihydrate complex a new apoptotic agent through Flk-1 down regulation, caspase-3 activation and oligonucleosomes DNA fragmentation.
Abstract New bis(acridine-9-carboxylate)-nitro-europium(III) dihydrate complex was synthesized and characterized. In vivo anti-angiogenic activities of bis(acridine-9-carboxylate)-nitro-europium(III) dihydrate complex against Ehrlich ascites carcinoma (EAC) cells are described. The newly synthesized complex resulted in inhibition of proliferation of EAC cells and ascites formation. The anti-tumor effect was found to be through anti-angiogenic activity as evident by the reduction of microvessel density in EAC solid tumors. The anti-angiogenic effect is mediated through down-regulation of VEGF receptor type-2 (Flk-1)...
Source: Bioorganic and Medicinal Chemistry - December 27, 2012 Category: Chemistry Authors: Azab HA, Hussein BH, El-Azab MF, Gomaa M, El-Falouji AI Tags: Bioorg Med Chem Source Type: research

Fusobacterium necrophorum mastoiditis in children – Emerging pathogen in an old disease
Conclusion: Our data support the literature suggesting that the occurrence of F. necrophorum mastoiditis among children is rising. Acute coalescent mastoiditis due to F. necrophorum is associated with a complicated course and warrants particular attention by pediatricians, infectious disease experts, and ear, nose and throat specialists.
Source: International Journal of Pediatric Otorhinolaryngology - December 27, 2012 Category: ENT & OMF Authors: Havatzelet Yarden-Bilavsky, Eyal Raveh, Gilat Livni, Oded Scheuerman, Jacob Amir, Efraim Bilavsky Tags: Research papers Source Type: research

Mentors And The Butterfly Effect: Triggers For Discovering Signalling by Proteinases via Proteinase-Activated Receptors (PARs) And More.
Mentors And The Butterfly Effect: Triggers For Discovering Signalling by Proteinases via Proteinase-Activated Receptors (PARs) And More. Clin Invest Med. 2012;35(6):E378 Authors: Hollenberg MD Abstract The essential role of proteinases as regulatory digestive enzymes, recognized since the late 1800s, has been underscored by the discovery that more than 2% of the genome codes for proteinases and their inhibitors. Further, by the early 1970s it was appreciated that in addition to their digestive actions, proteinases can affect cell function: (1) by the generation or degradation of peptide hormones and (2) by ...
Source: Clinical and Investigative Medicine - December 27, 2012 Category: Biomedical Science Authors: Hollenberg MD Tags: Clin Invest Med Source Type: research

The Influence of Swabbing Solutions on DNA Recovery from Touch Samples(,).
In this study, fingerprints were collected using swabs moistened with water or laboratory or commercially available detergents, including sodium dodecyl sulfate (SDS), Triton X-100, Tween 20, Formula 409(®) , and Simple Green(®) . Prints were swabbed, DNA isolated using an organic extraction, yields quantified, and relative yields compared. In all cases, the detergent-based swabbing solutions outperformed water, with SDS and Triton X-100 producing significant increases in yield. Short tandem repeat profiles were consistent with the individuals that placed them. Subsequent analysis of SDS concentrations for collecting tou...
Source: Journal of Forensic Sciences - December 27, 2012 Category: Forensic Medicine Authors: Thomasma SM, Foran DR Tags: J Forensic Sci Source Type: research

Performance evaluation of the Abbott CELL-DYN Emerald for use as a bench-top analyzer in a research setting.
CONCLUSION: The CELL-DYN Emerald was generally comparable to the larger reference analyzer for both human and mouse samples. It would be suitable for use in satellite research laboratories or as a backup system in larger laboratories. PMID: 23279758 [PubMed - as supplied by publisher]
Source: International Journal of Laboratory Hematology - December 27, 2012 Category: Hematology Authors: Khoo TL, Xiros N, Guan F, Orellana D, Holst J, Joshua DE, Rasko JE Tags: Int J Lab Hematol Source Type: research

Pertinence of the Sysmex XE-5000™ parameters: rule of slide review in a context of 'normal' lymphocyte count (defined from control and mantle cell lymphoma blood specimens).
CONCLUSION: Among the parameters provided by the Sysmex XE-5000™ analyzer, the combination of the alarm VAL_ABN LYMPH and the LyX value, routinely available on a simple blood analysis appears particularly informative to trigger slide review in a context of 'normal' lymphocyte count with a good sensitivity (85%) to detect circulating lymphoma cells and with <1% of false positive results. PMID: 23279807 [PubMed - as supplied by publisher]
Source: International Journal of Laboratory Hematology - December 27, 2012 Category: Hematology Authors: Chhuy J, Morel D, Goeddert G, Magaud JP, Felman P, Baseggio L Tags: Int J Lab Hematol Source Type: research

Clinical and molecular characterization of hemoglobin Maputo [beta 47 (CD6) Asp > Tyr HBB: c.142G > T] and G-Ferrara [beta 57 (E1) Asn > Lys HBB: c.174C > A] in a newborn screening in Brazil.
Clinical and molecular characterization of hemoglobin Maputo [beta 47 (CD6) Asp > Tyr HBB: c.142G > T] and G-Ferrara [beta 57 (E1) Asn > Lys HBB: c.174C > A] in a newborn screening in Brazil. Int J Lab Hematol. 2012 Dec 27; Authors: Silva MR, Sendin SM, Araujo IC, Pimentel FS, Viana MB PMID: 23279838 [PubMed - as supplied by publisher]
Source: International Journal of Laboratory Hematology - December 27, 2012 Category: Hematology Authors: Silva MR, Sendin SM, Araujo IC, Pimentel FS, Viana MB Tags: Int J Lab Hematol Source Type: research

Efficacy and safety of fluticasone/formoterol combination therapy in patients with moderate-to-severe asthma
Conclusion: Fluticasone/formoterol combination therapy will provide clinicians with an efficacious alternative treatment option for patients with moderate-to-severe asthma.
Source: Respiratory Medicine CME - December 27, 2012 Category: Respiratory Medicine Authors: Jonathan Corren, Lyndon E. Mansfield, Tetyana Pertseva, Viktor Blahzko, Kirsten Kaiser Tags: Asthma and Allergy Source Type: research

Pharmacokinetic Properties of Single-Dose Lamivudine/Adefovir Dipivoxil Fixed-Dose Combination in Healthy Chinese Male Volunteers.
CONCLUSIONS: The FDC capsule was bioequivalent to the concurrent administration of lamivudine + adefovir dipivoxil tablets based on the 90% CIs of the GMRs for C(max), AUC(0-∞), AUC(0-last), and t12 (all were between 0.80 and 1.25). Both treatments were well-tolerated. Clinicaltrial.gov identifier: NCT01353742. PMID: 23274144 [PubMed - as supplied by publisher]
Source: Clinical Therapeutics - December 27, 2012 Category: Drugs & Pharmacology Authors: Fok BS, Gardner S, Piscitelli S, Chen S, Chu TT, Chan JC, Tomlinson B Tags: Clin Ther Source Type: research

Reproducibility of Immunohistochemical Scoring for Epidermal Growth Factor Receptor Expression in Non-Small Cell Lung Cancer.
Conclusions.-After appropriate training, assessing EGFR expression by this immunohistochemistry-based method allowed a highly reproducible allocation of non-small cell lung cancers into clinically relevant high- or low-EGFR expression groups. PMID: 23270410 [PubMed - as supplied by publisher]
Source: Archives of Pathology and Laboratory Medicine - December 27, 2012 Category: Laboratory Medicine Authors: Rüschoff J, Kerr KM, Grote HJ, Middel P, von Heydebreck A, Alves VA, Baldus SE, Büttner R, Carvalho L, Fink L, Jochum W, Lo AW, López-Ríos F, Marx A, Molina TJ, Olszewski WT, Rieker RJ, Volante M, Thunnissen E, Wrba F, Celik I, Störkel S Tags: Arch Pathol Lab Med Source Type: research

Gene therapy for the prevention of vein graft disease.
Abstract Ischemic cardiovascular disease remains the leading cause of death worldwide. Despite advances in the medical management of atherosclerosis over the past several decades, many patients require arterial revascularization to reduce mortality and alleviate ischemic symptoms. Technological advancements have led to dramatic increases in the use of percutaneous and endovascular approaches, yet surgical revascularization (bypass surgery) with autologous vein grafts remains a mainstay of therapy for both coronary and peripheral artery disease. Although bypass surgery is highly efficacious in the short term, long-t...
Source: Translational Research : the journal of laboratory and clinical medicine - December 27, 2012 Category: Laboratory Medicine Authors: Southerland KW, Frazier SB, Bowles DE, Milano CA, Kontos CD Tags: Transl Res Source Type: research

MicroRNA regulation of cardiac conduction and arrhythmias.
This article aims to review the studies linking miRNAs to cardiac excitability and other processes pertinent to arrhythmia. PMID: 23274306 [PubMed - as supplied by publisher]
Source: Translational Research : the journal of laboratory and clinical medicine - December 27, 2012 Category: Laboratory Medicine Authors: Kim GH Tags: Transl Res Source Type: research

Evaluation of coagulation factors and platelet function from an off-line modified ultrafiltration technique for post-cardiopulmonary bypass circuit blood recovery.
This study evaluated the off-line MUF process of the Hemobag (HB, Global Blood Resources, Somers, CT, USA) to quantitate coagulation factor levels, platelet (PLT) count and function in one facility and cellular growth factor concentrations of the final product that were transfused to the patient in another facilityIn two cardiac surgery facilities, after decannulation, the extracorporeal circuit (ECC) blood from 22 patients undergoing cardiac surgery was processed with the HB device. In eleven patients from the first facility by the study design, blood samples for coagulation factor levels and PLT aggregation were drawn fr...
Source: Perfusion - December 27, 2012 Category: Cardiovascular & Thoracic Surgery Authors: Beckmann S, Lynn P, Miller S, Harris R, Dimarco R, Ross J Tags: Perfusion Source Type: research

Clinical and molecular characterization of hemoglobin Maputo [beta 47 (CD6) Asp > Tyr HBB: c.142G > T] and G‐Ferrara [beta 57 (E1) Asn > Lys HBB: c.174C > A] in a newborn screening in Brazil
Source: Clinical and Laboratory Haematology - December 27, 2012 Category: Hematology Authors: M. R. Silva, S. M. Sendin, I. C. O. Araujo, F. S. Pimentel, M. B. Viana Tags: Letter to the Editor Source Type: research

Performance evaluation of the Abbott CELL‐DYN Emerald for use as a bench‐top analyzer in a research setting
ConclusionThe CELL‐DYN Emerald was generally comparable to the larger reference analyzer for both human and mouse samples. It would be suitable for use in satellite research laboratories or as a backup system in larger laboratories.
Source: Clinical and Laboratory Haematology - December 27, 2012 Category: Hematology Authors: T.‐L. Khoo, N. Xiros, F. Guan, D. Orellana, J. Holst, D. E. Joshua, J. E. J. Rasko Tags: Original Article Source Type: research

A Common Marking Technique Affects Tadpole Behavior and Risk of Predation
We examined the consequences of a common larval amphibian marking technique (staining with methylene blue) for wood frog tadpole behavior and survival in an ecologically realistic scenario (predation). We measured a number of tadpole behavioral variables, under baseline conditions and in the presence of olfactory cues of a predator, for marked and unmarked tadpoles. We then exposed pairs of tadpoles (one marked and one unmarked) to one of two predators and tested for the effects of marking on the susceptibility of tadpoles to predation. We found that marking suppressed the increase in movement rate that typically occurred ...
Source: Ethology - December 27, 2012 Category: Zoology Authors: Bradley E. Carlson, Tracy Langkilde Tags: Research Paper Source Type: research

Implementation of an evidence based guideline reduces blood tests and length of stay for the limping child in a paediatric emergency department
Conclusion Implementation of an evidence based clinical practice guideline for the limping child in a paediatric ED reduced the overall time patients spent in the ED, reduced the need for unnecessary laboratory investigations and ensured that appropriate investigations were carried out on an individual patient basis.
Source: Emergency Medicine Journal - December 27, 2012 Category: Emergency Medicine Authors: McCanny, P. J., McCoy, S., Grant, T., Walsh, S., O'Sullivan, R. Tags: Original articles Source Type: research

Ovarian torsion among girls presenting with abdominal pain: a retrospective cohort study
Conclusion Clinical or laboratory features that would identify cases of OT among girls admitted with abdominal pain could not be identified. Some findings help to differentiate OT from appendicitis, but there is a large degree of overlap. OT is an uncommon condition, but has important management implications, and should be considered in all girls presenting with abdominal pain.
Source: Emergency Medicine Journal - December 27, 2012 Category: Emergency Medicine Authors: McCloskey, K., Grover, S., Vuillermin, P., Babl, F. E. Tags: Electronic pages Source Type: research

PyroTRF-ID: a novel bioinformatics methodology for the affiliation of terminal-restriction fragments using 16S rRNA gene pyrosequencing data
Conclusions: PyroTRF-ID profits from complementary advantages of pyrosequencing and T-RFLP and is particularly adapted for optimizing laboratory and computational efforts to describe microbial communities and their dynamics in any biological system. The high resolution of the microbial community composition is provided by pyrosequencing, which can be performed on a restricted set of selected samples, whereas T-RFLP enables simultaneous fingerprinting of numerous samples at relatively low cost and is especially adapted for routine analysis and follow-up of microbial communities on the long run.
Source: BMC Microbiology - Latest articles - December 27, 2012 Category: Microbiology Authors: David WeissbrodtNoam ShaniLucas SinclairGrégory LefebvrePierre RossiJulien MaillardJacques RougemontChristof Holliger Source Type: research

Recovery balance: a method for estimating losses in a Bacillus anthracis spore sampling protocol
ConclusionsThe approach used in this study pointed out the need for a closer analysis of the complex interaction between spores and wipe material because a substantial percentage of spores were lost to the wipe. Significance and Impact of the StudyThe recovery balance, in association with independent controls, provides an account for error contribution and potential variability on each step of the sampling protocol. The approach is not meant to be a replacement for field or laboratory validation of wipe recoveries but promote the development of new collection methodologies and support protocol optimization in laboratory settings.
Source: Journal of Applied Microbiology - December 27, 2012 Category: Microbiology Authors: S.M. Da Silva, A.A. Urbas, J.J. Filliben, J.B. Morrow Tags: Original Article Source Type: research

Cholestatic hepatitis C following liver transplantation: An outcome‐based histological definition, clinical predictors, and prognosis
In conclusion, we propose diagnostic criteria for cholestatic HCV that include specific criteria (the presence of at least 3 of the 4 histopathological features on biopsy) and other supportive and exclusionary criteria. Older donor age and rejection increase the risk of cholestatic HCV, and an elevation in the total bilirubin level may help to identify these patients. These criteria must be validated prospectively. Liver Transpl 19:78–88, 2013. © 2012 AASLD.
Source: Liver Transplantation - December 27, 2012 Category: Transplant Surgery Authors: Elizabeth C. Verna, Rita Abdelmessih, Marcela A. Salomao, Jay Lefkowitch, Roger K. Moreira, Robert S. Brown Tags: Original Article Source Type: research

Precision Diagnostics in Transplantation: From Bench to Bedside
The Canadian and American Societies of Transplantation held a symposium on February 22, 2012 in Quebec City focused on discovery, validation and translation of new diagnostic tools into clinical transplantation. The symposium focused on antibody testing, transplantation pathology, molecular diagnostics and laboratory support for the incompatible patient. There is an unmet need for more precise diagnostic approaches in transplantation. Significant potential for increasing the diagnostic precision in transplantation was recognized through the integration of conventional histopathology, molecular technologies and sensitive an...
Source: American Journal of Transplantation - December 27, 2012 Category: Transplant Surgery Authors: M. Mengel, P. Campbell, H. Gebel, P. Randhawa, E. R. Rodriguez, R. Colvin, J. Conway, R. Hachem, P. Halloran, S. Keshavjee, P. Nickerson, C. Murphey, J. O'Leary, J. Reeve, K. Tinckam, E. F. Reed Tags: Meeting Report Source Type: research

Surgical outcomes of severe tricuspid regurgitation: predictors of adverse clinical outcomes
Conclusions Long-term survival after TV surgery for severe TR was affected by several preoperative factors including advanced heart failure symptom, comorbidity, end-organ dysfunction and laboratory abnormalities, but not by the type of surgery or causes of TR.
Source: Heart - December 27, 2012 Category: Cardiology Authors: Kim, J. B., Jung, S.-H., Choo, S. J., Chung, C. H., Lee, J. W. Tags: Drugs: cardiovascular system, Right sided valvular heart disease, Epidemiology Cardiovascular surgery Source Type: research

Chapter 13: Mining Electronic Health Records in the Genomics Era
by Joshua C. Denny Abstract: The combination of improved genomic analysis methods, decreasing genotyping costs, and increasing computing resources has led to an explosion of clinical genomic knowledge in the last decade. Similarly, healthcare systems are increasingly adopting robust electronic health record (EHR) systems that not only can improve health care, but also contain a vast repository of disease and treatment data that could be mined for genomic research. Indeed, institutions are creating EHR-linked DNA biobanks to enable genomic and pharmacogenomic research, using EHR data for phenotypic information. However...
Source: PLoS Computational Biology - December 27, 2012 Category: Biology Authors: Joshua C. Denny Source Type: research

Introduction to spin label electron paramagnetic resonance spectroscopy of proteins
Abstract An undergraduate laboratory exercise is described to demonstrate the biochemical applications of electron paramagnetic resonance (EPR) spectroscopy. The β93 cysteine residue of hemoglobin is labeled by the covalent binding of 3‐maleimido‐proxyl (5‐MSL) and 2,2,5,5‐tetramethyl‐1‐oxyl‐3‐methyl methanethiosulfonate (MTSL), respectively. The excess spin label is removed by gel‐exclusion chromatography. Changes in the mobility of the reporter groups attached to the protein are monitored by EPR spectroscopy. While the spectral parameters of the rigidly attached 5‐MSL provide information on the rotat...
Source: Biochemistry and Molecular Biology Education - December 27, 2012 Category: Biochemistry Authors: Michelle Melanson, Abha Sood, Fanni Török, Marianna Török* Tags: Laboratory Exercise Source Type: research

Obesity and the prediction of minimal disease activity: A prospective study in psoriatic arthritis
ConclusionObesity is a negative predictor of achieving and maintaining MDA.
Source: Arthritis Care and Research - December 27, 2012 Category: Rheumatology Authors: Matteo Nicola Dario di Minno, Rosario Peluso, Salvatore Iervolino, Roberta Lupoli, Anna Russolillo, Raffaele Scarpa, Giovanni di Minno Tags: Special Theme Articles: Obesity and the Rheumatic Diseases Source Type: research

Protease-activated receptor-1 impairs host defense in murine pneumococcal pneumonia: a controlled laboratory study
Conclusion: PAR-1 impairs host defense during murine pneumococcal pneumonia.
Source: Critical Care - December 27, 2012 Category: Intensive Care Authors: Marcel SchoutenCornelis van 't VeerJoris RoelofsMarcel LeviTom van der Poll Source Type: research

Increasing ciprofloxacin resistance of isolates from infected urines of a cross-section of patients in Karachi
Conclusions: A decrease in bacterial susceptibility of uropathogens to ciprofloxacin, a commonly prescribed drug in our population, is underlined, occurring possibly due to overuse pressure. Empirical initial treatment with ciprofloxacin would be inadequate in more than half of UTI cases, thereby counseling increased C/S testing of urines to provide existing sensitivity data for apt drug prescription.
Source: BioMed Central - December 27, 2012 Category: Journals (General) Authors: Farhan Essa AbdullahAkhtar Amin MemonMuhammad Yasin BandukdaMarium Jamil Source Type: research

Adaptive evolution by recombination is not associated with increased mutation rates in Maize streak virus
Conclusions: While our results suggest that recombination does not strongly influence mutation rates in MSV, they indicate that high geminivirus mutation rates are at least partially attributable to increased susceptibility of all geminivirus genomes to oxidative damage while in a single stranded state.
Source: BioMed Central - December 27, 2012 Category: Journals (General) Authors: Adérito L MonjaneDaniel PandeFrancisco LakayDionne N ShepherdEric van der WaltPierre LefeuvreJean-Michel LettArvind VarsaniEdward P RybickiDarren P Martin Source Type: research

Doppler Tissue Imaging Provides an Estimate of Pulmonary Arterial Pressure in Children with Pulmonary Hypertension Due to Congenital Intracardiac Shunts
Conclusion.When used in conjunction with conventional methods, TE′/TA′ has the highest sensitivity and specificity in distinguishing between patients and healthy controls.
Source: Congenital Heart Disease - December 27, 2012 Category: Cardiology Authors: Ayhan Cevik, Serdar Kula, Rana Olgunturk, Berna Saylan, Ayhan Pektas, Deniz Oguz, Sedef Tunaoglu Tags: Original Article Source Type: research

A new experimental setup for making thermal emission measurements in a simulated lunar environment
I. R. Thomas, B. T. Greenhagen, N. E. Bowles, K. L. Donaldson Hanna, J. Temple et al. One of the key problems in determining lunar surface composition for thermal-infrared measurements is the lack of comparable laboratory-measured spectra. As the surface is typically composed of fine-grained particulates, the lunar environment induces a thermal gradient within the near sub-surface, a ... [Rev. Sci. Instrum. 83, 124502 (2012)] published Wed Dec 26, 2012.
Source: Review of Scientific Instruments - December 26, 2012 Category: Physics Source Type: research

Novel laser spectroscopic technique for continuous analysis of N(2) O isotopomers - application and intercomparison with isotope ratio mass spectrometry.
CONCLUSIONS: These findings validate QCLAS as a viable alternative technique with even higher precision than state-of-the-art IRMS. Thus, laser spectroscopy has the potential to contribute significantly to a better understanding of N turnover in soils, which is crucial for advancing strategies to mitigate emissions of this efficient greenhouse gas. Copyright © 2012 John Wiley & Sons, Ltd. PMID: 23239336 [PubMed - in process]
Source: Rapid Communications in Mass Spectrometry : RCM - December 26, 2012 Category: Chemistry Authors: Köster JR, Well R, Tuzson B, Bol R, Dittert K, Giesemann A, Emmenegger L, Manninen A, Cárdenas L, Mohn J Tags: Rapid Commun Mass Spectrom Source Type: research

Electron ionization of different large perfluoroethers embedded in ultracold helium droplets: effective freezing of short-lived decomposition intermediates.
CONCLUSIONS: Ionic fragmentation intermediates are frozen and subsequently stabilized in the He environment. Helium droplets can hence be viewed as a cryogenic laboratory transforming short-lived decomposition intermediates into stable fragment ions appearing as strong signals in the mass spectrum. Copyright © 2012 John Wiley & Sons, Ltd. PMID: 23239377 [PubMed - in process]
Source: Rapid Communications in Mass Spectrometry : RCM - December 26, 2012 Category: Chemistry Authors: Bartl P, Tanzer K, Mitterdorfer C, Karolczak S, Illenberger E, Denifl S, Scheier P Tags: Rapid Commun Mass Spectrom Source Type: research

β(2)-Microglobulin Amyloidosis.
Abstract Dialysis-related amyloidosis (DRA) is a clinical syndrome of pain, loss of function and other symptoms due to the deposition of amyloid consisting of β(2)-microglobulin (β(2)m) in the musculoskeletal system. The condition is seen in patients who suffer from chronic kidney disease and are treated with hemodialysis for a long time. Even though β(2)m easily can be manipulated to form amyloid in laboratory experiments under non-physiological conditions the precise mechanisms involved in the formation of β(2)m-amyloid in patients with DRA have been difficult to unravel. The current knowledge which is review...
Source: Sub-Cellular Biochemistry - December 26, 2012 Category: Biochemistry Authors: Corlin DB, Heegaard NH Tags: Subcell Biochem Source Type: research

Evaluation of a Method to Quantify Quercetin Aglycone in Onion (Allium cepa) by Single- and Multi-laboratory Validation Studies.
Abstract Official Methods of Analysis of AOAC International (OMA) 2006.07 was originally designed for quantifying flavonol aglycones in ginkgo dietary supplements. To determine whether the method is applicable to the quantification of flavonol aglycones in lyophilized onion samples, single- and multi-laboratory validation studies were performed. Triplicated measurements on 3 different days revealed that the mean quercetin content was 3.48 g/kg dry weight, and the relative repeatability standard deviation (RSD(r)) and the relative intermediate standard deviation (RSD(int)) were 0.8 and 1.8%, respectively. The recove...
Source: Analytical Sciences : the international journal of the Japan Society for Analytical Chemistry - December 26, 2012 Category: Chemistry Authors: Watanabe J, Takebayashi J, Takano-Ishikawa Y, Yasui A Tags: Anal Sci Source Type: research

The consortium for the standardization of influenza seroepidemiology (CONSISE): a global partnership to standardize influenza seroepidemiology and develop influenza investigation protocols to inform public health policy
CONSISE – The consortium for the Standardization of Influenza Seroepidemiology – is a global partnership to develop influenza investigation protocols and standardize seroepidemiology to inform health policy. This international partnership was formed in 2011 and was created out of a need, identified during the 2009 H1N1 pandemic, for timely seroepidemiological data to better estimate pandemic virus infection severity and attack rates to inform policy decisions. CONSISE has developed into a consortium of two interactive working groups: epidemiology and laboratory, with a steering committee composed of individuals from se...
Source: Influenza and Other Respiratory Viruses - December 26, 2012 Category: Infectious Diseases Authors: Maria D. Kerkhove, Eeva Broberg, Othmar G. Engelhardt, John Wood, Angus Nicoll, Tags: Short Article Source Type: research

Chromosome heteromorphisms are more frequent in couples with recurrent abortions.
Chromosome heteromorphisms are more frequent in couples with recurrent abortions. Genet Mol Res. 2012;11(4):3847-51 Authors: Akbaş H, Isi H, Oral D, Türkyılmaz A, Kalkanlı-Taş S, Simşek S, Balkan M, Sakar MN, Fidanboy M, Alp MN, Budak T Abstract Chromosomal heteromorphism is considered a variant of a normal karyotype, but it is more frequent in couples with repeated miscarriages. We investigated chromosomal heteromorphism in couples with repeated miscarriages in comparison with a control group. A total of 455 couples who applied to our genetic diagnosis laboratory in Diyarbakir, Turkey, were evaluated...
Source: Genetics and Molecular Research - December 26, 2012 Category: Genetics & Stem Cells Authors: Akbaş H, Isi H, Oral D, Türkyılmaz A, Kalkanlı-Taş S, Simşek S, Balkan M, Sakar MN, Fidanboy M, Alp MN, Budak T Tags: Genet Mol Res Source Type: research

Using SPME fibers and Tenax to predict the bioavailability of pyrethroids and chlorpyrifos in field sediments.
This study compared three methods to assess the bioavailability and ultimately toxicity of pyrethroid pesticides including field-deployed SPME fibers, laboratory-exposed SPME fibers, and a 24-h Tenax extraction. The objective of the current study was to compare the ability of these methods to quantify the bioavailable fraction of pyrethroids in contaminated field sediments that were toxic to benthic invertebrates. In general, Tenax proved a more sensitive method than SPME fibers and a correlation between Tenax extractable concentrations and mortality was observed. PMID: 23202281 [PubMed - in process]
Source: Environmental Pollution - December 26, 2012 Category: Environmental Health Authors: Harwood AD, Landrum PF, Weston DP, Lydy MJ Tags: Environ Pollut Source Type: research

Enhanced degradation of (14)C-HCB in two tropical clay soils using multiple anaerobic-aerobic cycles.
Abstract The aim of the study was to induce and enhance the degradation of hexachlorobenzene (HCB), a highly-chlorinated persistent organic pollutant, in two ecologically different tropical soils: a paddy soil (PS) and a non-paddy soil (FS). The degradation of HCB was enhanced using two anaerobic-aerobic cycles in model laboratory experiments. There was greater degradation of HCB in the PS (half-life of 224 days) relative to the FS (half-life of 286 days). It was further shown that soils amended with compost had higher metabolite concentrations relative to the non-amended soils. In the first cycle, there was little...
Source: Environmental Pollution - December 26, 2012 Category: Environmental Health Authors: Kengara FO, Doerfler U, Welzl G, Ruth B, Munch JC, Schroll R Tags: Environ Pollut Source Type: research

Assessment of potential climate change impacts on peatland dissolved organic carbon release and drinking water treatment from laboratory experiments.
Abstract Catchments draining peat soils provide the majority of drinking water in the UK. Over the past decades, concentrations of dissolved organic carbon (DOC) have increased in surface waters. Residual DOC can cause harmful carcinogenic disinfection by-products to form during water treatment processes. Increased frequency and severity of droughts combined with and increased temperatures expected as the climate changes, have potentials to change water quality. We used a novel approach to investigate links between climate change, DOC release and subsequent effects on drinking water treatment. We designed a climate...
Source: Environmental Pollution - December 26, 2012 Category: Environmental Health Authors: Tang R, Clark JM, Bond T, Graham N, Hughes D, Freeman C Tags: Environ Pollut Source Type: research

Experience of pediatric stroke from a tertiary medical center in North India
Conclusion: AIS is more common than ICH in this study and 8.9% patients died. Infection is the most common cause of pediatric stroke in Northern India which is amenable to therapy and is preventable.
Source: Journal of the Neurological Sciences - December 26, 2012 Category: Neurology Authors: Jayantee Kalita, Gourav Goyal, Usha Kant Misra Tags: Original Articles Source Type: research

Pyoderma Gangrenosum With Wrist Joint Destruction: Case Report
Pyoderma gangrenosum (PG) is a rare, noninfectious, neurotrophic dermatosis. We observed a case of PG mimicking cutaneous and osteoarticular infections that presented with a prolonged ulcer on the forearm, severe wrist pain, anemia, substantial local and systemic inflammation as evaluated by serum laboratory data, and carpal osteolysis. Although PG rarely damages joints, the ulcer extended to the joint and destroyed the osteochondral tissues. Advanced ulcerative colitis, which is a most common comorbidity of PG, proved to be an underlying disease. Antibiotic and surgical treatment did not heal the ulcer, which was successf...
Source: The Journal of Hand Surgery - December 26, 2012 Category: Surgery Authors: Hyonmin Choe, Hiroaki Sakano, Hidetake Takigami, Yutaka Inaba, Kosuke Matsuo, Tomoyuki Saito Tags: Scientific Articles Source Type: research

Retracted: Selective use of hand and forearm muscles during mini-implant insertion: a natural torquimeter [Scientific Section]
Conclusions: Digital torque was 42% smaller than brachial torque, and it was mechanically safer and biologically more compatible, allowing fracture prevention of 1·5 mm or thicker mini-implant diameter due to insertion torque limitation at 15 N/cm.
Source: Journal of Orthodontics - December 26, 2012 Category: Dentistry Authors: Estelita, S., Janson, G., Chiqueto, K., Ferreira, E., Janson, M. Tags: Scientific Section Source Type: research