LipidologyThis is an RSS file. You can use it to subscribe to this data in your favourite RSS reader, such as GoogleReader, or to display this data on your own website or blog. Subscribe to this data using MyMedWorm.Subscribe to this data using GoogleReader.Subscribe to this data using Bloglines.Subscribe to this data using MyYahoo.

This page shows you the most recent publications within this specialty of the MedWorm directory.

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30

Eating fish linked to slight decrease in coronary calcification riskEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Eating fish is associated with a slightly decreased risk for coronary calcification in older adults, particularly women, research indicates. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 19, 2010 Category: Lipidology Source Type: news

Hemodynamic parameters differ in ability to identify atherosclerotic plaqueEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hemodynamic parameters differ in their ability to identify sites prone to atherosclerosis in the right coronary artery, study findings suggest. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 19, 2010 Category: Lipidology Source Type: news

Lipid ratios may be ‘simple, integrated’ CVD risk markersEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Three lipid ratios are strongly and consistently associated with the metabolic syndrome, insulin resistance, and adiponectin, a Korean study suggests. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 18, 2010 Category: Lipidology Source Type: news

Rosuvastatin cuts LDL-C in children with familial hypercholesterolemiaEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rosuvastatin therapy significantly reduces levels of low-density lipoprotein cholesterol and is well-tolerated in adolescents with familial hypercholesterolemia, a randomized placebo-controlled trial has found. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 18, 2010 Category: Lipidology Source Type: news

Pitavastatin treatment reduces marker of oxidative stressEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Pitavastatin treatment brings about beneficial changes in serum markers of oxidative stress, a study in people with hypercholesterolemia has found. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 18, 2010 Category: Lipidology Source Type: news

Dietary meat fats and burden of cardiovascular disease risk factors, in the elderly; a report from the MEDIS studyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Dietary fats have long been associated with human health, and especially cardiovascular disease (CVD). Some observational studies have shown that reduction in dietary fats, and particularly cholesterol is associated with lower cardiovascular risk; however, other prospective studies or randomized controlled trials of dietary fat reduction or modification have shown varying results on CVD morbidity and mortality. In this work we evaluated the relationships between dietary fats and a cluster of CVD risk factors (i.e., diabetes, obesity, hypercholesterolemia, hypertension), among elderly individuals without known CVD. In parti...
Source: Lipids in Health and Disease - March 18, 2010 Category: Lipidology Authors: Evangelos PolychronopoulosGeorge PounisVassiliki BountzioukaAkis ZeimbekisIoanna TsiligianniBrikena-Eirini QiraEfthimios GotsisGeorge MetallinosChristos LionisDemosthenes Panagiotakos Source Type: journals

Adipocytes play key role in HDL hemostasis in vivoEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Researchers have shown, for the first time, that adipose tissue plays a role in maintaining levels of high-density lipoprotein cholesterol, acting through the ATP-binding cassette subfamily A member 1 and scavenger receptor class B type I cholesterol transporters. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 17, 2010 Category: Lipidology Source Type: news

Lipid levels affect cognitive function more in early than late old ageEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
High lipid levels are a greater risk factor for cognitive dysfunction before the age of 65 years than afterwards, report researchers. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 17, 2010 Category: Lipidology Source Type: news

Mipomersen effective therapy for reducing LDL cholesterol in FH patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Treatment with the antisense inhibitor of apolipoprotein B synthesis mipomersen is a novel and effective way of reducing low-density lipoprotein cholesterol in patients with homozygous familial hypercholesterolemia, say researchers. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 16, 2010 Category: Lipidology Source Type: news

ACCORD lipid: Fibrates do not improve CV outcomes in diabetic patients taking statinsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Combination therapy with fenofibrate and simvastatin does not provide additional protection against cardiovascular disease in high-risk patients with Type 2 diabetes compared with simvastatin alone, according to findings from the ACCORD lipid trial. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 15, 2010 Category: Lipidology Source Type: news

Severe hypertriglyceridemia linked with excess CHD, stroke mortalityEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Severe hypertriglyceridemia is associated with a markedly increased risk for coronary heart disease and stroke mortality, even in the absence of diabetes, a UK study shows. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 15, 2010 Category: Lipidology Source Type: news

Eprotirome helps reduce lipids in statin-treated patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Treatment with the thyroid hormone analogue eprotirome produces significant additional reductions in low-density lipoprotein cholesterol in statin-treated patients with dyslipidemia, report researchers. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 15, 2010 Category: Lipidology Source Type: news

Physicians fail to assess LDL cholesterol targets correctly for many patientsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Only about half of all high-risk patients are assigned the correct low-density lipoprotein targets by their primary care physicians, report researchers. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 13, 2010 Category: Lipidology Source Type: news

Adipose-secreted signals associated with different clinical BP phenotypesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Normal blood pressure and white-coat hypertension are associated with a more favorable circulating adipokine pattern than masked or sustained hypertension, Greek researchers report. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 12, 2010 Category: Lipidology Source Type: news

Implications for lipids during replication of enveloped viruses.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Enveloped viruses, which include many medically important viruses such as Human Immunodeficiency Virus, influenza virus and Hepatitis C virus, are intracellular parasites that acquire lipid envelopes from their host cells. Success of replication is intimately linked to their ability to hijack host cell mechanisms, particularly those related to membrane dynamics and lipid metabolism. Despite recent progress, our knowledge of lipid mediated virus-host interactions remains highly incomplete. In addition, diverse experimental systems are used to study different stages of virus replication thus complicating comparisons. Thi...
Source: Chemistry and Physics of Lipids - March 12, 2010 Category: Lipidology Authors: Chan RB, Tanner L, Wenk MR Tags: Chem Phys Lipids Source Type: journals

Metabolic syndrome associated with subclinical CVDEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
People with the metabolic syndrome are at increased risk for asymptomatic cardiovascular disease, a community-based study has found. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 12, 2010 Category: Lipidology Source Type: news

Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on-line MS identificationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The seed lipid of Nitraria tangutorum was extracted by supercritical carbon dioxide extraction, microwave-assisted reflux extraction, ultrasound-assisted extraction, or solvent reflux extraction. The experimental parameters of supercritical carbon dioxide extraction including pressure, temperature, particle size, and extraction time were investigated. A facile and sensitive method for the simultaneous determination of 30 saturated and 9 unsaturated fatty acids by HPLC with fluorescence detection after pre-column derivatization was developed. Fatty acid derivatives were separated on a reversed-phase Eclipse XDB-C8 column in...
Source: European Journal of Lipid Science and Technology - March 12, 2010 Category: Lipidology Authors: Yourui Suo, Lingyun Wang Source Type: journals

Unbound DHA causes a high blank value in [beta]-oxidation assay: a concern for in vitro studiesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, seven [1-14C] FAs were mixed with albumin and assayed for [beta]-oxidation in rat liver homogenates. In the process of identifying the radioactive background of control assay by LCMS/MS, the results indicated different binding capacity of FAs to albumin. The percentage of unbound FAs recovered in clarified acidic solution was lower than 2% with 16:0 and 18:1n-9, nearly 5% with EPA, 7% with 18:2n-6, 18:3n-3 and 20:4n-6, and surprisingly high to 41% with DHA. Various FA/albumin molar ratios as well as different types of albumin only marginally affected these data. Thus, the big mass of unbound free DHA led to ...
Source: European Journal of Lipid Science and Technology - March 12, 2010 Category: Lipidology Authors: Zhen-Yu Du, Pedro Araujo, Ingunn Stubhaug, Livar Frøyland Source Type: journals

Policosanol characterization and accumulation during ripening of Tunisian Olea europaea L. fruitsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Policosanol is a mixture of bioactive molecules shown to have beneficial effects in treating hypercholesterolemia. Food products enriched in policosanol are currently available in the US market. In the present study, eight policosanol components were identified by GC-MS during the ripening of Meski olives. The quantitative characterization of these compounds was performed using GC-FID. The results showed that the maximum level of total policosanol components (947.20 mg/100 g oil) was reached at the 26th week after the flowering date of Meski olives. Hexacosanol and tetracosanol were the predominant policosanol components a...
Source: European Journal of Lipid Science and Technology - March 12, 2010 Category: Lipidology Authors: Faouzi Sakouhi, Sadok Boukhchina, Christelle Absalon, Eric Fouquet, Habib Kallel Source Type: journals

Effect of triacylglycerol species on the crystallizing behavior of a model water/oil emulsionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined whether the texture degradation is related to the fatty acid formula and the triglyceride formula by carbon number. We also examined the effect of the triglyceride species concentration estimated from the fatty acid formula on the texture degradation. The palmitic acid content was related with texture degradation at high concentration among the fatty acid species. The triglyceride content was not related with texture degradation. Triacylglycerol species with palmitic acid such as tripalmitate (PPP) and 1,3-dipalmitoyl-2-oleoyl-glycerol (POP) were related with texture degradation. The summed up concentration of ...
Source: European Journal of Lipid Science and Technology - March 12, 2010 Category: Lipidology Authors: Leo Tanaka, Tomoyuki Isogai, Susumu Miura, Mototake Murakami Source Type: journals

Identification and characterization of the n-6 fatty acid-producing Mucor rouxii native isolate CFR-G15Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In zygomycetes fungi, many Mucor spp. have been known to produce [gamma]-linolenic acid (GLA) in their biomass. Among 250 soil samples screened, 20 Mucor isolates showed GLA in their mycelial mass under normal cultivation conditions. Sudan Black B was used for screening their qualitative oleaginesity. Among the representative isolates, Mucor sp. CFR-G15, when grown in a fat-producing medium, showed a maximum lipid content of 30 ± 1.32% in its mycelia and 14.42 ± 0.74% GLA. By using gene-specific primers, the 18S rRNA gene and the [Delta]6 DES gene were amplified by PCR technique. The nucleotide sequences of the 18S rRNA ...
Source: European Journal of Lipid Science and Technology - March 12, 2010 Category: Lipidology Authors: Shivaramu S. Mamatha, Prakash M. Halami, Govindarajulu Venkateswaran Source Type: journals

Proficiency test on the determination of mineral oil in sunflower oilEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Following the discovery of mineral oil contamination of Ukrainian sunflower oil in April 2008, the Institute for Reference Materials and Measurements (IRMM) of the European Commission's Joint Research Centre (JRC) was requested by the Directorate General Health and Consumers (DG SANCO) to organise a proficiency test on the determination of mineral oil in sunflower oil. The aim of this test was to evaluate the comparability of analysis results gained by laboratories in the EU and the Ukraine. The organisation of the study and the evaluation of the results were done in accordance with "The International Harmonised Protocol f...
Source: European Journal of Lipid Science and Technology - March 12, 2010 Category: Lipidology Authors: Lubomir Karasek, Thomas Wenzl, Franz Ulberth Source Type: journals

NPC1L1 inhibitor ezetimibe is a reliable therapeutic agent for non-obese patients with nonalcoholic fatty liver diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Background: We recently examined the distribution of abdominal fat, dietary intake and biochemical data in patients with nonalcoholic fatty liver disease (NAFLD) and found that non-obese NAFLD patients did not necessarily exhibit insulin resistance and/or dysregulated secretion of adipocytokines. However, dietary cholesterol intake was superabundant in non-obese patients compared with obese patients, although total energy and carbohydrate intake was not excessive. Therefore, excess cholesterol intake appears to be one of the main factors associated with NAFLD development and liver injury. Methods: We reviewed a year of fol...
Source: Lipids in Health and Disease - March 12, 2010 Category: Lipidology Authors: Munechika EnjojiKuzuyuki MachidaMotoyuki KohjimaMasaki KatoKazuhiro KotohKazuhisa MatsunagaManabu NakashimaMakoto Nakamuta Source Type: journals

Sustained LDL inhibition may contribute to dialysis PAD benefitsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Sustained inhibition of low-density lipoprotein may contribute to long-term benefits in dialysis patients with peripheral arterial disease who respond to apheresis, Japanese researchers suggest. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 11, 2010 Category: Lipidology Source Type: news

Association of LIPC -250G>A polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populationsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to detect the association of LIPC -250G>A (rs2070895) polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations. Methods: A total of 778 subjects of Bai Ku Yao and 648 participants of Han Chinese aged 15-80 were randomly selected from our previous stratified randomized cluster samples. Genotyping of the LIPC -250G>A was performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis, and then confirmed by direct sequencing. Results: The levels of serum total cholesterol (TC), HDL-C, low-de...
Source: Lipids in Health and Disease - March 11, 2010 Category: Lipidology Authors: Li MengYin RuixingLi YiyangLong XingjiangLi KelaLiu WanyingZhang LinLin WeixiongYang DezhaiPan Shangling Source Type: journals

Direct measurement of cholesterol levels comparable to standard methodsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Direct measurement of both high- and low-density lipoprotein cholesterol using modern assay techniques reveals similar levels to those found using standard methods, say US researchers. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 10, 2010 Category: Lipidology Source Type: news

Combined oral contraceptives have neutral impact on LDL levelsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Oral contraceptives containing both estrogen and progestin induce an increase in the production of apolipoprotein B-containing lipoproteins, including low-density lipoprotein, a kinetic study has found. (Source: MedWire News - Lipidology)
Source: MedWire News - Lipidology - March 10, 2010 Category: Lipidology Source Type: news

Evaluation of different formulas for LDL-C calculationEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Our modified formula for LDL-C estimation appears to be more accurate than both Friedewald's and Anandaraja's formulas when applied to a Serbian population. (Source: Lipids in Health and Disease)
Source: Lipids in Health and Disease - March 10, 2010 Category: Lipidology Authors: Ana VujovicJelena Kotur-StevuljevicSlavica SpasicNada BujisicJelena MartinovicMilica VujovicVesna Spasojevic-KalimanovskaAleksandra ZeljkovicDragoljub Pajic Source Type: journals

ERRATA [Errata]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: The Journal of Lipid Research)
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Tags: Errata Source Type: journals

Direct quantitative determination of ceramide glycosylation in vivo: a new approach to evaluate cellular enzyme activity of glucosylceramide synthase [Methods]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Glucosylceramide synthase (GCS or GlcT-1), converting ceramide to glucosylceramide, is a key enzyme for the synthesis of glycosphingolipids. Due to its diverse roles in physiology and diseases, GCS may be a disease marker and drug target. Current assays for enzymes including GCS are based on reactions conducted in a test tube using enzyme preparations. Measurement of enzyme activity in laboratory-made conditions cannot directly evaluate the role of GCS in cells. Here, we introduce a new approach to determine GCS cellular activity using fluorescent NBD C6-ceramide in vivo. Cellular GCS transfers UDP-glucose to NBD C6-cerami...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Gupta, V., Patwardhan, G. A., Zhang, Q.-J., Cabot, M. C., Jazwinski, S. M., Liu, Y.-Y. Tags: Methods Source Type: journals

Separation and characterization of cardiolipin molecular species by reverse-phase ion pair high-performance liquid chromatography-mass spectrometry [Methods]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An improved high-performance liquid chromatography-mass spectrometry method for the separation and characterization of cardiolipin molecular species is presented. Reverse-phase ion pair chromatography with acidified triethylamine resulted in increased chromatographic retention and resolution when compared with chromatography without acidified triethylamine. Using a hybrid triple quadrupole linear ion trap mass spectrometer to generate MS/MS spectra revealed three regions within each spectrum that could be used to deduce the structure of the cardiolipin molecular species: the diacylglycerol phosphate region, the monoacylgly...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Minkler, P. E., Hoppel, C. L. Tags: Methods Source Type: journals

Alternative splicing attenuates transgenic expression directed by the apolipoprotein E promoter-enhancer based expression vector pLIV11 [Methods]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The plasmid vector pLIV11 is used commonly to achieve liver-specific expression of genes of interest in transgenic mice and rabbits. Expression is driven by the human apolipoprotein (apo)E 5' proximal promoter, which includes 5 kb of upstream sequence, exon 1, intron 1, and 5 bp of exon 2. A 3.8 kb 3' hepatic control region, derived from a region ~18 kb downstream of the apoE gene, enhances liver-specific expression. Here, we report that cDNA sequences inserted into the multiple cloning site (MCS) of pLIV11, which is positioned just downstream of truncated exon 2, can cause exon 2 skipping. Hence, splicing is displaced to ...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Cheng, D., MacArthur, P. S., Rong, S., Parks, J. S., Shelness, G. S. Tags: Methods Source Type: journals

APOC1 T45S polymorphism is associated with reduced obesity indices and lower plasma concentrations of leptin and apolipoprotein C-I in aboriginal Canadians [Patient-Oriented and Epidemiological Research]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Apolipoprotein (apo) C-I is a constituent of chylomicrons, very low density lipoprotein, and high density lipoprotein. The role of apo C-I in human metabolism is incompletely defined. We took advantage of a naturally occurring amino acid polymorphism that is present in aboriginal North Americans, namely apo C-I T45S. We assessed the hypothesis that metabolic traits, including obesity-related and lipoprotein-related traits, would differ between carriers and noncarriers of apo C-I T45S. A genotyping assay was developed for APOC1 T45S and genotypes were determined in a sample of 410 Canadian Oji-Cree subjects. The allele freq...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Lahiry, P., Cao, H., Ban, M. R., Pollex, R. L., Mamakeesick, M., Zinman, B., Harris, S. B., Hanley, A. J. G., Huff, M. W., Connelly, P. W., Hegele, R. A. Tags: Patient-Oriented and Epidemiological Research Source Type: journals

Glucose stimulates cholesterol 7{alpha}-hydroxylase gene transcription in human hepatocytes [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study uncovers a novel link between hepatic glucose metabolism and bile acid synthesis. Glucose induction of bile acid synthesis may have an important implication in metabolic control of glucose, lipid, and energy homeostasis under normal and diabetic conditions. (Source: The Journal of Lipid Research)
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Li, T., Chanda, D., Zhang, Y., Choi, H.-S., Chiang, J. Y. L. Tags: Research Articles Source Type: journals

Quantitation of serum angiopoietin-like proteins 3 and 4 in a Finnish population sample [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We have developed and validated quantitative ELISAs for human angiopoietin-like (ANGPTL)3 and 4 and correlated their serum levels with parameters of lipid and carbohydrate metabolism. For this study, we used a random subsample of the Health 2000 Health Examination Survey consisting of 125 men and 125 women, aged 30–94 years. The anthropometric and biochemical parameters of subjects were characterized in detail. ANGPTL 3 and 4 levels were determined using the developed ELISAs. The intra- and inter-assay coefficients of variation for the assays were less than 15%. The average serum concentration of ANGPTL3 was 368 &plu...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Robciuc, M. R., Tahvanainen, E., Jauhiainen, M., Ehnholm, C. Tags: Research Articles Source Type: journals

Marked accumulation of 27-hydroxycholesterol in SPG5 patients with hereditary spastic paresis [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Patients with a recessively inherited "pure" hereditary spastic paresis (SPG5) have mutations in the gene coding for the oxysterol 7 hydroxylase (CYP7B1). One of the expected metabolic consequences of such mutations is accumulation of oxysterol substrates due to decreased enzyme activity. In accordance with this, we demonstrate here that four patients with the SPG5 disease have 6- to 9-fold increased plasma levels of 27-hydroxycholesterol. A much higher increase, 30- to 50-fold, was found in cerebrospinal fluid. The plasma levels of 25-hydroxycholesterol were increased about 100-fold. There were no measurable levels of thi...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Schule, R., Siddique, T., Deng, H.-X., Yang, Y., Donkervoort, S., Hansson, M., Madrid, R. E., Siddique, N., Schols, L., Bjorkhem, I. Tags: Research Articles Source Type: journals

Disruption of the C-terminal helix by single amino acid deletion is directly responsible for impaired cholesterol efflux ability of apolipoprotein A-I Nichinan [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Apolipoprotein A-I (apoA-I) Nichinan, a naturally occurring variant with E235 in the C terminus, is associated with low plasma HDL levels. Here, we investigated the tertiary structure, lipid-binding properties, and ability to induce cellular cholesterol efflux of apoA-I Nichinan and its C-terminal peptide. Thermal and chemical denaturation experiments demonstrated that the E235 mutation decreased the protein stability compared with wild type (WT). ApoA-I Nichinan exhibited capabilities to bind to or solubilize lipid vesicles that are intermediate to that of WT and a L230P/L233P/Y236P variant in which the C-terminal -helix ...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Kono, M., Tanaka, T., Tanaka, M., Vedhachalam, C., Chetty, P. S., Nguyen, D., Dhanasekaran, P., Lund-Katz, S., Phillips, M. C., Saito, H. Tags: Research Articles Source Type: journals

Photoactivable sphingosine as a tool to study membrane microenvironments in cultured cells [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Human fibroblasts from normal subjects and Niemann-Pick A (NPA) disease patients were fed with two labeled metabolic precursors of sphingomyelin (SM), [3H]choline and photoactivable sphingosine, that entered into the biosynthetic pathway allowing the synthesis of radioactive phosphatidylcholine and SM, and of radioactive and photoactivable SM ([3H]SM-N3). Detergent resistant membrane (DRM) fractions prepared from normal and NPA fibroblasts resulted as highly enriched in [3H]SM-N3. However, lipid and protein analysis showed strong differences between the two cell types. After cross-linking, different patterns of SM-protein ...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Aureli, M., Prioni, S., Mauri, L., Loberto, N., Casellato, R., Ciampa, M. G., Chigorno, V., Prinetti, A., Sonnino, S. Tags: Research Articles Source Type: journals

Analysis of lipid transfer activity between model nascent HDL particles and plasma lipoproteins: implications for current concepts of nascent HDL maturation and genesis [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The specifics of nascent HDL remodeling within the plasma compartment remain poorly understood. We developed an in vitro assay to monitor the lipid transfer between model nascent HDL (LpA-I) and plasma lipoproteins. Incubation of -125I-LpA-I with plasma resulted in association of LpA-I with existing plasma HDL, whereas incubation with TD plasma or LDL resulted in conversion of -125I-LpA-I to preβ-HDL. To further investigate the dynamics of lipid transfer, nascent LpA-I were labeled with cell-derived [3 H]cholesterol (UC) or [3H]phosphatidylcholine (PC) and incubated with plasma at 37°C. The majority of UC and PC w...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Bailey, D., Ruel, I., Hafiane, A., Cochrane, H., Iatan, I., Jauhiainen, M., Ehnholm, C., Krimbou, L., Genest, J. Tags: Research Articles Source Type: journals

FXR activation reverses insulin resistance and lipid abnormalities and protects against liver steatosis in Zucker (fa/fa) obese rats [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we investigated the effect of FXR activation by 6-ethyl-chenodeoxycholic acid, (6E-CDCA, 10 mg/kg) on insulin resistance and liver and muscle lipid metabolism in fa/fa rats and compared its activity with rosiglitazone (10 mg/kg) alone or in combination with 6E-CDCA (5 mg/kg each). In comparison to lean (fa/+), fa/fa rats on a normal diet developed insulin resistance and liver steatosis. FXR activation protected against body weight gain and liver and muscle fat deposition and reversed insulin resistance as assessed by insulin responsive substrate-1 phosphorylation on serine 312 in liver and muscles. Activatio...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Cipriani, S., Mencarelli, A., Palladino, G., Fiorucci, S. Tags: Research Articles Source Type: journals

Liver X receptor activation promotes macrophage-to-feces reverse cholesterol transport in a dyslipidemic hamster model [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Liver X receptor (LXR) activation promotes reverse cholesterol transport (RCT) in rodents but has major side effects (increased triglycerides and LDL-cholesterol levels) in species expressing cholesteryl ester transfer protein (CETP). In the face of dyslipidemia, it remains unclear whether LXR activation stimulates RCT in CETP species. We therefore used a hamster model made dyslipidemic with a 0.3% cholesterol diet and treated with vehicle or LXR agonist GW3965 (30 mg/kg bid) over 10 days. To investigate RCT, radiolabeled 3H-cholesterol macrophages or 3H-cholesteryl oleate-HDL were then injected to measure plasma and feces...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Briand, F., Treguier, M., Andre, A., Grillot, D., Issandou, M., Ouguerram, K., Sulpice, T. Tags: Research Articles Source Type: journals

Baseline cholesterol absorption and the response to ezetimibe/simvastatin therapy: a post-hoc analysis of the ENHANCE trial [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Subjects with increased cholesterol absorption might benefit more from statin therapy combined with a cholesterol absorption inhibitor. We assessed whether baseline cholesterol absorption markers were associated with response to ezetimibe/simvastatin therapy, in terms of LDL-cholesterol (LDL-C) lowering and cholesterol absorption inhibition, in patients with familial hypercholesterolemia (FH). In a posthoc analysis of the two-year ENHANCE trial, we assessed baseline cholesterol-adjusted campesterol (campesterol/TC) and sitosterol/TC ratios in 591 FH patients. Associations with LDL-C changes and changes in cholesterol absor...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Jakulj, L., Vissers, M. N., Groen, A. K., Hutten, B. A., Lutjohann, D., Veltri, E. P., Kastelein, J. J. P. Tags: Research Articles Source Type: journals

Myeloperoxidase and serum amyloid A contribute to impaired in vivo reverse cholesterol transport during the acute phase response but not group IIA secretory phospholipase A2 [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, our data demonstrate that acute inflammation impairs cholesterol efflux in patients and macrophage-to-feces RCT in vivo in mice. Myeloperoxidase and SAA contribute to a certain extent to reduced RCT during inflammation but not sPLA2. However, reduced bile acid formation and decreased biliary sterol excretion might represent major contributing factors to decreased RCT in inflammation. (Source: The Journal of Lipid Research)
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Annema, W., Nijstad, N., Tolle, M., de Boer, J. F., Buijs, R. V. C., Heeringa, P., van der Giet, M., Tietge, U. J. F. Tags: Research Articles Source Type: journals

A novel role for ABCA1-generated large pre-{beta} migrating nascent HDL in the regulation of hepatic VLDL triglyceride secretion [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In Tangier disease, absence of ATP binding cassette transporter A1 (ABCA1) results in reduced plasma HDL and elevated triglyceride (TG) levels. We hypothesized that hepatocyte ABCA1 regulates VLDL TG secretion through nascent HDL production. Silencing of ABCA1 expression in oleate-stimulated rat hepatoma cells resulted in: 1) decreased large nascent HDL (>10 nm diameter) and increased small nascent HDL (<10 nm) formation, 2) increased large buoyant VLDL1 particle secretion, and 3) decreased phosphatidylinositol-3 (PI3) kinase activation. Nascent HDL-containing conditioned medium from rat hepatoma cells or HEK293 cell...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Chung, S., Gebre, A. K., Seo, J., Shelness, G. S., Parks, J. S. Tags: Research Articles Source Type: journals

Modulation of the activity of cytosolic phospholipase A2{alpha} (cPLA2{alpha}) by cellular sphingolipids and inhibition of cPLA2{alpha} by sphingomyelin [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined the effect of the cellular sphingolipid level on the release of arachidonic acid (AA) and activity of cytosolic phospholipase A2 (cPLA2) using two Chinese hamster ovary (CHO)-K1-derived mutants deficient in sphingolipid synthesis: LY-B cells defective in the LCB1 subunit of serine palmitoyltransferase for de novo synthesis of sphingolipid species, and LY-A cells defective in the ceramide transfer protein CERT for SM synthesis. When LY-B and LY-A cells were cultured in Nutridoma medium and the sphingolipid level was reduced, the release of AA stimulated by the Ca2+ ionophore A23187 increased 2-fold and 1.7-fold,...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Nakamura, H., Wakita, S., Suganami, A., Tamura, Y., Hanada, K., Murayama, T. Tags: Research Articles Source Type: journals

CGI-58/ABHD5 is a coenzyme A-dependent lysophosphatidic acid acyltransferase [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Mutations in human CGI-58/ABHD5 cause Chanarin-Dorfman syndrome (CDS), characterized by excessive storage of triacylglycerol in tissues. CGI-58 is an /β-hydrolase fold enzyme expressed in all vertebrates. The carboxyl terminus includes a highly conserved consensus sequence (HXXXXD) for acyltransferase activity. Mouse CGI-58 was expressed in Escherichia coli as a fusion protein with two amino terminal 6-histidine tags. Recombinant CGI-58 displayed acyl-CoA-dependent acyltransferase activity to lysophosphatidic acid, but not to other lysophospholipid or neutral glycerolipid acceptors. Production of phosphatidic acid inc...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Montero-Moran, G., Caviglia, J. M., McMahon, D., Rothenberg, A., Subramanian, V., Xu, Z., Lara-Gonzalez, S., Storch, J., Carman, G. M., Brasaemle, D. L. Tags: Research Articles Source Type: journals

INSIG1 influences obesity-related hypertriglyceridemia in humans [Research Articles]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In our analysis of a quantitative trait locus (QTL) for plasma triglyceride (TG) levels [logarithm of odds (LOD) = 3.7] on human chromosome 7q36, we examined 29 single nucleotide polymorphisms (SNPs) across INSIG1, a biological candidate gene in the region. Insulin-induced genes (INSIGs) are feedback mediators of cholesterol and fatty acid synthesis in animals, but their role in human lipid regulation is unclear. In our cohort, the INSIG1 promoter SNP rs2721 was associated with TG levels (P = 2 x 10–3 in 1,560 individuals of the original linkage cohort, P = 8 x 10–4 in 920 unrelated individuals of the replicati...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Smith, E. M., Zhang, Y., Baye, T. M, Gawrieh, S., Cole, R., Blangero, J., Carless, M. A., Curran, J. E., Dyer, T. D., Abraham, L. J., Moses, E. K., Kissebah, A. H., Martin, L. J., Olivier, M. Tags: Research Articles Source Type: journals

Lipid second messengers and related enzymes in vertebrate rod outer segments [Thematic Reviews]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Rod outer segments (ROSs) are specialized light-sensitive organelles in vertebrate photoreceptor cells. Lipids in ROS are of considerable importance, not only in providing an adequate environment for efficient phototransduction, but also in originating the second messengers involved in signal transduction. ROSs have the ability to adapt the sensitivity and speed of their responses to ever-changing conditions of ambient illumination. A major contributor to this adaptation is the light-driven translocation of key signaling proteins into and out of ROS. The present review shows how generation of the second lipid messengers fr...
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Giusto, N. M., Pasquare, S. J., Salvador, G. A., Ilincheta de Boschero, M. G. Tags: Thematic Reviews Source Type: journals

The acute phase response inhibits reverse cholesterol transport [Commentary]Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Source: The Journal of Lipid Research)
Source: The Journal of Lipid Research - March 9, 2010 Category: Lipidology Authors: Feingold, K. R., Grunfeld, C. Tags: Commentary Source Type: journals