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Enamel matrix derivative enhances tissue formation around scaffolds used for tissue engineering of ligamentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The following in vitro translational study investigated whether enamel matrix derivative (EMD), an approved biomimetic treatment for periodontal disease (Emdogain®) and hard-to-heal wounds (Xelma®), enhanced synovial cell colonization and protein synthesis around a scaffold used clinically for in situ tissue engineering of the torn anterior cruciate ligament (ACL). Synovial cells were enzymatically extracted from bovine synovium and dynamically seeded onto polyethylene terephthalate (PET) scaffolds. The cells were cultured in low-serum medium (0.5% FBS) for 4 weeks with either a single administration of EMD at the start ...
Source: Journal of Tissue Engineering and Regenerative Medicine - November 21, 2009 Category: Molecular Biology Authors: Michael P. Messenger, El M. Raïf, Bahaa B. Seedhom, Steven J. Brookes Source Type: journals

Investigating the importance of flow when utilizing hyaluronan scaffolds for tissue engineeringEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study also highlighted the possibility that in high-density tissue culture with a biodegradable component, dynamic conditions may be more conducive to optimal tissue development than the static environment because they facilitate the efficient removal of high concentrations of degradation end-products accumulating in the pericellular space. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Journal of Tissue Engineering and Regenerative Medicine)
Source: Journal of Tissue Engineering and Regenerative Medicine - November 20, 2009 Category: Molecular Biology Authors: Gail C. Donegan, John A. Hunt, Nicholas Rhodes Source Type: journals

Enhanced treatment of articular cartilage defect of the knee by intra-articular injection of Bcl-xL-engineered mesenchymal stem cells in rabbit modelEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined whether the transduction of an anti-apoptotic protein, Bcl-xL, into MSCs could prevent cell death and improve the implantation efficiency of MSCs in a rabbit model. Our current findings demonstrate that the group treated with Bcl-xL-engineered MSCs could improve cartilage healing both morphologically and histologically when compared with the controls. These results suggest that intra-articular injection of Bcl-xL-engineered MSCs is a potential non-invasive therapeutic method for effectively treating cartilage defects of the knee. Copyright © 2009 John Wiley & Sons, Ltd. (Source: Journal of Tissue Engineering a...
Source: Journal of Tissue Engineering and Regenerative Medicine - November 20, 2009 Category: Molecular Biology Authors: Bin Hu, Jun-Lin Ren, Jun-Rui Zhang, Qin Ma, Yan-Pu Liu, Tian-Qiu Mao Source Type: journals

The stability of imine-containing dynamic [2]rotaxanes to hydrolysisEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ken Cham-Fai Leung, Wing-Yan Wong, Fabio Arico, Philip C. Haussmann, J. Fraser Stoddart (Paper from Org. Biomol. Chem.) Ken Cham-Fai Leung, Org. Biomol. Chem., 2010, DOI: 10.1039/b915864b To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 20, 2009 Category: Molecular Biology Authors: Ken Cham-Fai LeungWing-Yan Wong Fabio Arico Philip C. Haussmann J. Fraser Stoddart Source Type: journals

Conditional gene deletion reveals functional redundancy of GABAB receptors in peripheral nociceptors in vivoEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This study addressed contribution of GABAB receptors expressed on primary afferent nociceptive fibers to the modulation of pain. We observed that neither the development of acute and chronic pain nor the analgesic effects of a systematically-delivered GABAB agonist was significantly changed upon a specific deletion of GABAB receptors from peripheral nociceptive neurons in vivo. This lets us conclude that GABAB receptors in the peripheral nervous system play a less important role than those in the central nervous system in the regulation of pain. (Source: Molecular Pain)
Source: Molecular Pain - November 19, 2009 Category: Molecular Biology Authors: Vijayan GangadharanNitin AgarwalStefan BruggerImgard TegederBernhard BettlerRohini KunerMartina Kurejova Source Type: journals

Cerebrospinal fluid tau and ptau181 increase with cortical amyloid deposition in cognitively normal individuals: Implications for future clinical trials of Alzheimer's diseaseEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Alzheimer's disease (AD) pathology is estimated to develop many years before detectable cognitive decline. Fluid and imaging biomarkers may identify people in early symptomatic and even preclinical stages, possibly when potential treatments can best preserve cognitive function. We previously reported that cerebrospinal fluid (CSF) levels of amyloid-[beta]42 (A[beta]42) serve as an excellent marker for brain amyloid as detected by the amyloid tracer, Pittsburgh compound B (PIB). Using data from 189 cognitively normal participants, we now report a positive linear relationship between CSF tau/ptau181 (primary constituents of ...
Source: EMBO Molecular Medicine - November 19, 2009 Category: Molecular Biology Authors: Anne M. Fagan, Mark A. Mintun, Aarti R. Shah, Patricia Aldea, Catherine M. Roe, Robert H. Mach, Daniel Marcus, John C. Morris, David M. Holtzman Source Type: journals

Diversification of the cullin familyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Most of the known types of cullin-containing ubiquitin ligase complexes are ancient. The available data suggest that, since the origin of eukaryotes, complex diversity has been mostly generated by combining closely related subunits, while radical innovations, giving rise to novel types of complexes, have been scarce. However, several protist groups not examined so far contain highly divergent cullins, indicating that additional types of complexes may exist. (Source: BMC Evolutionary Biology - Latest articles)
Source: BMC Evolutionary Biology - Latest articles - November 19, 2009 Category: Molecular Biology Authors: Ignacio Marin Source Type: journals

Development of a new microwave-assisted cleavable backbone amide linker (BAL): a comparative studyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Stijn Claerhout, Thibault Duchene, Dirk Tourwe, Erik V. Van der Eycken (Communication from Org. Biomol. Chem.) Stijn Claerhout, Org. Biomol. Chem., 2010, DOI: 10.1039/b919365k To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 19, 2009 Category: Molecular Biology Authors: Stijn ClaerhoutThibault Duchene Dirk Tourwe Erik V. Van der Eycken Source Type: journals

Phosphorane intermediate vs. leaving group stabilization by intramolecular hydrogen bonding in the cleavage of trinucleoside monophosphates: implications for understanding catalysis by the large ribozymesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Tuomas Lonnberg, Maarit Laine (Paper from Org. Biomol. Chem.) Tuomas Lonnberg, Org. Biomol. Chem., 2010, DOI: 10.1039/b912042d To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 19, 2009 Category: Molecular Biology Authors: Tuomas LonnbergMaarit Laine Source Type: journals

Study on the selectivity in the electrophilic monofluorination of 2,3-allenoates with Selectfluor[trade mark sign]: an efficient synthesis of 4-fluoro-2(5H)-furanones and 3-fluoro-4-oxo-2(E)-alkenoatesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Bo Lu, Chunling Fu, Shengming Ma (Paper from Org. Biomol. Chem.) Bo Lu, Org. Biomol. Chem., 2010, DOI: 10.1039/b917793k To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 19, 2009 Category: Molecular Biology Authors: Bo LuChunling Fu Shengming Ma Source Type: journals

Translational advances and novel therapies for pancreatic ductal adenocarcinoma: hope or hype?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Review ArticlesSreenivasa Chandana, Daruka Mahadevan, Expert Reviews in Molecular Medicine, Volume 11 , pp e34AbstractBiological complexity, inaccessible anatomical location, nonspecific symptoms, lack of a screening biomarker, advanced disease at presentation and drug resistance epitomise pancreatic ductal adenocarcinoma (PDA) as a poor-prognosis, lethal disease. Twenty-five years of research (basic, translational and clinical) have barely made strides to improve survival, mainly because of a fundamental lack of knowledge of the biological processes initiating and propagating PDA. However, isolation of pancreas cancer ste...
Source: Expert Reviews in Molecular Medicine - November 18, 2009 Category: Molecular Biology Source Type: journals

Tissue persistence of fumonisin B1 in ducks and after exposure to a diet containing the maximum European tolerance for fumonisins in avian feeds.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Toxicity and persistence of fumonisin B1 (FB1) in liver, kidney and muscle were investigated in ducks fed 5, 10 and 20mg FB1+FB2/kg feed during force-feeding. Mortality and signs of toxicity were only obtained with 20mg/kg, whereas an increased Sa/So ratio was observed from 5mg/kg on. Persistence of FB1 was only found in liver (16 and 20 microg FB1/kg liver in ducks fed 10 and 20 mg FB1+FB2/kg feed, respectively). Toxicokinetic studies were conducted by the intravenous route (IV, single dose: 10mg FB1/kg body weight) and the oral route (single dose: 100mg FB1/kg body weight), in growing ducks and in ducks during force-...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Tardieu D, Bailly JD, Benlashehr I, Auby A, Jouglar JY, Guerre P Tags: Chem Biol Interact Source Type: journals

Glucocorticoid and adrenalectomy effects on the rat aryl hydrocarbon receptor pathway depend on the dosing regimen and post-surgical time.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates the effects of aromatic hydrocarbons, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin and 3-methylcholanthrene (MC); the prototypical response is induction of drug-metabolizing enzymes. Factors that regulate AHR levels in vivo are poorly understood and it is also not clear how AHR levels affect aromatic hydrocarbon responsiveness. Our interest in pituitary-dependent regulation of AHR levels was prompted by two findings from our laboratory: (1) hypophysectomized rats have reduced hepatic levels of AHR protein; and (2) glucocorticoid...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Mullen Grey AK, Riddick DS Tags: Chem Biol Interact Source Type: journals

l-Arginine supplementation induces glutathione synthesis in interscapular brown adipose tissue through activation of glutamate-cysteine ligase expression: The role of nitric oxide.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We examined whether nitric oxide (NO) in vivo could induce interscapular brown adipose tissue (IBAT) glutathione synthesis. Data show that NO induces in vivo IBAT glutathione synthesis through activation of glutamate-cysteine ligase (GCL) mRNA and protein expression. This NO effect appeared to be mediated by nuclear factor-kappaB (NF-kappaB) activation. We have also observed a complex series of in vivo cellular responses during chronic inhibition of NO synthesis, suggesting that regulatory pathways unrelated to GCL alteration underlie glutathione level increase induced by N(omega)-nitro-l-arginine methyl ester (l-NAME). In...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Petrović V, Buzadzić B, Korać A, Vasilijević A, Janković A, Korać B Tags: Chem Biol Interact Source Type: journals

(-)-Epigallocatechin gallate prevents carbon tetrachloride-induced rat hepatic fibrosis by inhibiting the expression of the PDGFRbeta and IGF-1R.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Hepatic fibrosis is a major complication of various chronic liver diseases. Activated hepatic stellate cells (HSCs) play a critical role in the development of liver fibrosis and the axis of platelet-derived growth factor (PDGF)/PDGF receptor (PDGFR), a member of receptor tyrosine kinases (RTKs), is closely associated with the activation of HSC. Insulin-like growth factor (IGF)-1 receptor (IGF-1R), which also belongs to RTKs, interacts with the PDGF/PDGFR axis, thereby cooperatively promoting hepatic fibrosis. We herein examined the effects of (-)-epigallocatechin gallate (EGCG), which inhibits the activation of several...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Yasuda Y, Shimizu M, Sakai H, Iwasa J, Kubota M, Adachi S, Osawa Y, Tsurumi H, Hara Y, Moriwaki H Tags: Chem Biol Interact Source Type: journals

Antioxidant activity and free radical scavenging reactions of hydroxybenzyl alcohols. Biochemical and pulse radiolysis studies.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Comparative studies on free radical scavenging by isomers of hydroxybenzyl alcohols (HBAs) were carried out to understand the molecular mechanisms involved in the antioxidant action. Using rat liver mitochondria as model systems, we have examined the radioprotective and antioxidant effects of hydroxybenzyl alcohols. Apart from their ability to scavenge free radicals and ferric reducing power, HBAs have shown good protection against radiation and oxidative stress. Using peroxyl radicals as initiator of reactive oxygen species (ROS), studies were carried out to evaluate antioxidant properties of HBAs against rat liver mi...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Dhiman SB, Kamat JP, Naik DB Tags: Chem Biol Interact Source Type: journals

Fetal arsenic exposure appears to facilitate endocrine disruption by postnatal diethylstilbestrol in neonatal mouse adrenal.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Fetal exposure of mice to arsenic and subsequent postnatal diethylstilbestrol (DES) facilitates production of urogenital system and liver tumors in the offspring when they reach adulthood. The adrenal is a target of endocrine disruption that could influence tumor formation at other sites. Thus, we examined possible fetal arsenic-induced adrenal effects as a potential basis of arsenic enhancement of DES carcinogenesis. Pregnant CD1 mice were given drinking water containing 85 ppm arsenic as sodium arsenite or unaltered water from day 8 to day 18 of gestation and were allowed to deliver normally. Groups of offspring were...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Liu J, Yu L, Coppin JF, Tokar EJ, Diwan BA, Waalkes MP Tags: Chem Biol Interact Source Type: journals

Novel nitro-oxy derivatives of celecoxib for the regulation of colon cancer cell growth.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Celecoxib is a non-steroidal anti-inflammatory drug (NSAID) developed as a selective inhibitor of cyclooxygenase-2 (COX-2). Despite the associated cardiovascular toxicity risk, celecoxib has been found to be effective in reducing cancer risk in animal and human studies. In the present study the antiproliferative activity of novel nitro-oxy-methyl substituted analogues of celecoxib (NO-cel), potentially less cardiotoxic, has been investigated in vitro on human colon cancer cells and compared with action of the parent drug. Moreover, experiments were performed in order to evaluate whether COX-2 pharmacological inhibition...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Bozzo F, Bassignana A, Lazzarato L, Boschi D, Gasco A, Bocca C, Miglietta A Tags: Chem Biol Interact Source Type: journals

Dual action of phenylarsine oxide on the glucose transport activity of GLUT1.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study investigated the effects of PAO on glucose uptake in L929 fibroblast cells, cells, which express only GLUT1. The data presented here reveal a dual effect of PAO. At low concentrations or short exposure times PAO stimulated glucose uptake reaching a peak activation of about 400% at 3 microM. At higher concentrations (40 microM), PAO clearly inhibited glucose uptake. At intermediate concentrations (10 microM), PAO had no effect under basal conditions but completely inhibited activation of glucose uptake by glucose deprivation and partially inhibited methylene blue-stimulated glucose uptake. PAO increased the speci...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Scott J, Opejin A, Tidball A, Stehouwer N, Rekman J, Louters LL Tags: Chem Biol Interact Source Type: journals

Chronic myelogenous leukemia and benzene exposure: a systematic review and meta-analysis of the case-control literature.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Benzene exposure is well demonstrated as a cause of acute myelogenous leukemia, but not of chronic myelogenous leukemia. Previous literature reviews based on case series and cohort studies have not shown an association. We have now conducted a literature search for case-control studies that examine the association between benzene exposure and chronic myelogenous leukemia. Six case-control studies have been found. These derive from occupational groups, cancer registries, and a clinical laboratory. Their exposure ascertainments are all based on job histories, job-exposure matricies, or industrial hygiene data. The odds r...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Lamm SH, Engel A, Joshi KP, Byrd DM, Chen R Tags: Chem Biol Interact Source Type: journals

Modulatory role of lipoic acid on lipopolysaccharide-induced oxidative stress in adult rat Sertoli cells in vitro.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Inflammatory reactions to microbial infections may cause male infertility. The mechanisms of inhibition of spermatogenesis can be studied in vitro using rat Sertoli cells. Bacterial lipopolysaccharides (LPS) induce acute inflammations. So LPS treated Sertoli cells can be used to test for new therapeutic compounds. The present study aimed to investigate the protective efficacy of dl-alpha-lipoic acid (LA) on lipopolysaccharide (LPS)-induced oxidative stress in adult rat Sertoli cells. Sertoli cells were divided into 4 groups. Group I served as a control incubated with water (vehicle). Groups II and IV were incubated wit...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Aly HA, Lightfoot DA, El-Shemy HA Tags: Chem Biol Interact Source Type: journals

Superoxide anion mediated mitochondrial dysfunction leads to hepatocyte apoptosis preferentially in the periportal region during copper toxicity in rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Chronic exposure to copper induces hepatocellular apoptosis with greater injury in the periportal region compared to the perivenous region. Here we have identified the factors responsible for the development of regional damage in the liver under in vivo conditions. Enhanced production of reactive oxygen species (ROS) with predominance of superoxide radical (O(2)(-)) indicates the contribution of redox imbalance in the process. This may be linked with copper catalyzed oxidation of GSH to GSSG resulting in the generation of O(2)(-). Downregulation of Cu-Zn SOD in consequence of the degradation of this enzyme, causes decr...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Roy DN, Mandal S, Sen G, Biswas T Tags: Chem Biol Interact Source Type: journals

Oxaliplatin-induced gamma-H2AX activation via both p53-dependent and -independent pathways but is not associated with cell cycle arrest in human colorectal cancer cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we investigated the roles of p53 and gamma-H2AX following oxaliplatin treatment, as they are important effector proteins for apoptosis and DSB repair, respectively. Both phosphorylated-p53 (Ser-15) and gamma-H2AX were up-regulated and accumulated in the nuclei of p53-wild type human colorectal cancer HCT116 cells after exposure to oxaliplatin. Concomitantly, oxaliplatin-induced G2/M arrest was associated with a reduction in both cyclin B1 expression and phosphorylated-CDC2 (Thr-161). Release of G2/M arrest by caffeine was accompanied by a decrease in the levels of p53/p21; however, gamma-H2AX levels were unc...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Chiu SJ, Lee YJ, Hsu TS, Chen WS Tags: Chem Biol Interact Source Type: journals

Synergistic increases of metabolism and oxidation-reduction genes on their expression after combined treatment with a CYP1A inducer and andrographolide.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We previously reported that andrographolide greatly enhanced the expression of CYP1A1. Since andrographolide is a major constituent of Andrographis paniculata, which has been employed for centuries in Asia and Europe as a folk remedy, we further analyzed genes whose expression was modified by andrographolide using primary-cultured mouse hepatocytes in a microarray assay. With the threshold for modification set at 2-fold, andrographolide up-regulated 18 genes among 28,853 genes, most of them related to metabolism/oxidation/reduction. Meanwhile, 5 genes, related to protein binding or calcium ion binding, were down-regula...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Chatuphonprasert W, Jarukamjorn K, Kondo S, Nemoto N Tags: Chem Biol Interact Source Type: journals

Inhibition of proliferation of a hepatoma cell line by fucoxanthin in relation to cell cycle arrest and enhanced gap junctional intercellular communication.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we hypothesized that fucoxanthin may cause cell cycle arrest and enhance gap junctional intercellular communication (GJIC) in SK-Hep-1 human hepatoma cells. Data revealed that fucoxanthin (1-20microM) strongly and concentration-dependently inhibited the proliferation of SK-Hep-1 cells at 24h of incubation, whereas fucoxanthin facilitated the growth of a murine embryonic hepatic (BNL CL.2) cells at 24h of incubation and only slightly slowed the cell proliferation at 48h. In SK-Hep-1 cells, fucoxanthin caused cell cycle arrest at G0/G1 phase and induced cell apoptosis, as evidenced by increased subG1 cells and...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Liu CL, Huang YS, Hosokawa M, Miyashita K, Hu ML Tags: Chem Biol Interact Source Type: journals

Altered pH homeostasis modulates the glutathione peroxidase mimics and other antioxidant properties of diphenyl diselenide.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recent evidences have shown that the in vivo antioxidant chemistry of organoselenium compounds such as diphenyl diselenide (DPDS) is complex and it is not completely understood. The complexity is partly due to the fact that DPDS is generally thought to exert its antioxidant action by mimicking glutathione peroxidase (GPx) with concomitant utilization of glutathione (GSH) in vitro. In contrast to in vitro data, we recently observed that DPDS increases rather than diminish GSH levels in diabetic models. The present study therefore sought to investigate a possible change in the antioxidant mechanisms of DPDS in changing p...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Ogunmoyole T, Rocha JB, Okoronkwo AE, Kade IJ Tags: Chem Biol Interact Source Type: journals

Mechanism of action of novel naphthofuranquinones on rat liver microsomal peroxidation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In order to elucidate the effect on mammal systems of new derivatives from 2-hydroxy-3-allyl-naphthoquinone, alpha-iodinated naphthofuranquinone (NPPN-3223), beta-iodinated naphthofuranquinone (NPPN-3222) and beta-methyl naphthofuranquinone (NPPN-3226) synthesized as possible trypanocidal agents, their effect on rat liver microsomal lipid peroxidation was investigated. They (a) inhibited NADPH-dependent, iron-catalyzed microsomal rat liver lipid peroxidation; (b) did not inhibit the tert-butyl hydroperoxide-dependent lipid peroxidation; (c) did not inhibit ascorbate-lipid peroxidation with the exception of NPPN-3226 wh...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Elingold I, Taboas MI, Casanova MB, Galleano M, Silva RS, Menna-Barreto RF, Ventura Pinto A, de Castro SL, Costa LE, Dubin M Tags: Chem Biol Interact Source Type: journals

Cytotoxic, cytoprotective and antioxidant activities of resveratrol and analogues in C6 astroglioma cells in vitro.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Resveratrol (3,4',5-trans-trihydroxystilbene) and other hydroxystilbenes exhibit in vitro antioxidant as well as prooxidant effects. The antioxidant properties are assumed to enable these compounds to protect cells from oxidative damage. The prooxidant effects are held likely to be responsible for their cytotoxic, anti-proliferative or pro-apoptotic effects observed in vitro. Regarding antioxidant/prooxidant activities in the past various studies were performed aiming at defining structure-activity relationships for hydroxystilbenes using cell-free systems. In the present study cultured C6 glioma cells were used in ord...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Rüweler M, Gülden M, Maser E, Murias M, Seibert H Tags: Chem Biol Interact Source Type: journals

Non-dioxin-like-PCBs phosphorylate Mdm2 at Ser166 and attenuate the p53 response in HepG2 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study we investigated the effects of 20 NDL-PCBs (concentration range 0.01-10microM) on pMdm2 Ser166, pErk Tyr204 and p53 on HepG2 cells. Six of the NDL-PCBs induced pErk Tyr204 and pMdm3 Ser166. This effect correlated with lowered basal levels of p53, as well as with an attenuated p53 response induced by etoposide and leptomycin B. Similar effects were induced by TCDD and the DL-PCB 126. We conclude that both DL-PCBs and NDL-PCBs in low concentrations can induce alterations in p53 signaling and that these effects can be correlated to rat liver carcinogenesis. PMID: 19751709 [PubMed - indexed for MEDLINE] (Sour...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Al-Anati L, Högberg J, Stenius U Tags: Chem Biol Interact Source Type: journals

Herb-drug interaction of silymarin or silibinin on the pharmacokinetics of trazodone in rats.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, the present study finds no marked effects of silymarin and silibinin on the pharmacokinetics of trazodone under normal daily doses and the relative safety of taking the herb with trazodone. PMID: 19765569 [PubMed - indexed for MEDLINE] (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Chang JC, Wu YT, Lee WC, Lin LC, Tsai TH Tags: Chem Biol Interact Source Type: journals

Hyper- and hypo-induction of cytochrome P450 activities with Aroclor 1254 and 3-methylcholanthrene in Cyp1a2(-/-) mice.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The response of hepatic mono-oxygenase activities to Aroclor 1254 or 3-methylcholanthrene was investigated in wild-type and Cyp1a2(-/-) mice. Cytochrome P450 concentrations were similar in naïve Cyp1a2(-/-) and wild-type mice. There was no difference between naïve wild-type and Cyp1a2(-/-) animals in 7-ethoxyresorufin and 7-ethoxy-4-trifluoromethylcoumarin dealkylase activities, nor was the induction response after 3-methylcholanthrene any different between the two genotypes. However, both activities were induced to a higher extent in Cyp1a2(-/-) mice after Aroclor 1254. In contrast, 7-pentoxyresorufin dealky...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Barker ML, Hathaway LB, Arch DD, Westbroek ML, Kushner JP, Phillips JD, Franklin MR Tags: Chem Biol Interact Source Type: journals

Effect of permethrin insecticide on rat polymorphonuclear neutrophils.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In conclusion, immunotoxicity of permethrin measured in rats could prompt future studies on the consequences of chronic insecticide exposure. PMID: 19772857 [PubMed - indexed for MEDLINE] (Source: Chemico-Biological Interactions)
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Gabbianelli R, Falcioni ML, Nasuti C, Cantalamessa F, Imada I, Inoue M Tags: Chem Biol Interact Source Type: journals

Effect of alpha tocopherol acetate in Walker 256/B cells-induced oxidative damage in a rat model of breast cancer skeletal metastases.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The pathophysiological changes and the oxidative-antioxidative status were evaluated in the bone microenvironment of rat inoculated with Walker 256/B mammary gland carcinoma cells, and used alpha-tocopherol acetate (ATA) as a countermeasure. Walker 256/B cells were injected into the right femora of aged male rats. Animals were randomized into three groups: 12 rats were injected with saline (control group); 14 rats were injected with Walker 256/B cells (5x10(4)) in the medullar cavity (W256 group); 14 rats were inoculated with Walker 256/B cells and treated with ATA (45mg/kg BW) (W256+ATA group). After 20 days, rats wer...
Source: Chemico-Biological Interactions - November 18, 2009 Category: Molecular Biology Authors: Badraoui R, Blouin S, Moreau MF, Gallois Y, Rebai T, Sahnoun Z, Baslé M, Chappard D Tags: Chem Biol Interact Source Type: journals

Fragment C of tetanus toxin, more than a carrier. Novel perspectives in non-viral ALS gene therapyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Abstract The non-toxic carboxy-terminal fragment of tetanus toxin heavy chain (TTC) has been implicated in the activation of cascades responsible for trophic actions and neuroprotection by inhibition of apoptosis. Previous in vitro studies have described signalling pathways that underlie the administration of TTC to neurons. We investigated whether these properties were maintained in a mouse model of neurodegenerative disease. Naked DNA encoding for TTC was injected intramuscularly and neuromuscular function and clinical behaviour were monitored until endstage in the transgenic SOD1G93A mouse model that expr...
Source: Journal of Molecular Medicine - November 18, 2009 Category: Molecular Biology Tags: Journal of Molecular Medicine Source Type: journals

A novel porcine gene, MAPKAPK3, is differentially expressed in the pituitary gland from mini-type Diannan small-ear pigs and large-type Diannan small-ear pigs.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The mRNA differential display technique was performed to investigate the differences of gene expression in the pituitary gland from mini-type Diannan small-ear pigs and large-type Diannan small-ear pigs. One novel gene differentially expressed was identified through semi-quantitative RT-PCR and its cDNA complete sequence was then obtained using the rapid amplification of cDNA ends (RACE) method. Nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence analysis revealed that the open reading frame of this gene encodes a protein of 384 amino acids has high homology with the mitoge...
Source: Molecular Biology Reports - November 18, 2009 Category: Molecular Biology Authors: Yonggang L Tags: Mol Biol Rep Source Type: journals

Effective relief of neuropathic pain by adeno-associated virus-mediated expression of a small hairpin RNA against GTP cyclohydrolase 1Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Therefore, the data suggests that GCH1 levels can be reduced by introducing rAAV-shGCH1, leading to pain relief. Based on the results, we propose that GCH1 modulation may be developed as a clinically applicable gene therapy strategy to treat neuropathic pain. (Source: Molecular Pain)
Source: Molecular Pain - November 18, 2009 Category: Molecular Biology Authors: Sung Jin KimWon Il LeeYoon Sun LeeDong Hou KimJin Woo ChangSeong Who KimHeuiran Lee Source Type: journals

Different forms of glycine- and GABAA-receptor mediated inhibitory synaptic transmission in mouse superficial and deep dorsal horn neuronsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Together these data show that Gly- and GABAARs with clearly differing physiological properties and cannabinoid-sensitivity contribute to fast synaptic inhibition in mouse SDH and DDH. (Source: Molecular Pain)
Source: Molecular Pain - November 18, 2009 Category: Molecular Biology Authors: Wayne AndersonBrett GrahamNatalie BeveridgePaul TooneyAlan BrichtaRobert Callister Source Type: journals

Oxaliplatin-induced loss of phosphorylated heavy neurofilament subunit neuronal immunoreactivity in rat DRG tissueEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Background: Oxaliplatin and related chemotherapeutic drugs cause painful chronic peripheral neuropathies in cancer patients. We investigated changes in neuronal size profiles and neurofilament immunoreactivity in L5 dorsal root ganglion (DRG) tissue of adult female Wistar rats after multiple-dose treatment with oxaliplatin, cisplatin, carboplatin or paclitaxel. Results: After treatment with oxaliplatin, phosphorylated neurofilament heavy subunit (pNF-H) immunoreactivity was reduced in neuronal cell bodies, but unchanged in nerve fibres, of the L5 DRG. Morphometric analysis confirmed significant changes in the number (-75%;...
Source: Molecular Pain - November 18, 2009 Category: Molecular Biology Authors: Stephen JamiesonJoshuan SubramaniamJohnson LiuNancy JongVirginia IpBronwen ConnorMark McKeage Source Type: journals

Phylogenomic analysis of the cystatin superfamily in eukaryotes and prokaryotesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: This study challenges the current view on the classification, origin and evolution of the cystatin superfamily and provides valuable insights into their functional diversification. The findings of this comprehensive study provide guides for future structural and evolutionary studies of the cystatin superfamily as well as of other protease inhibitors and proteases. (Source: BMC Evolutionary Biology - Latest articles)
Source: BMC Evolutionary Biology - Latest articles - November 18, 2009 Category: Molecular Biology Authors: Dusan KordisVito Turk Source Type: journals

Direct fixation of [11C]-CO2 by amines: formation of [11C-carbonyl]-methylcarbamatesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Alan A. Wilson, Armando Garcia, Sylvain Houle, Neil Vasdev (Paper from Org. Biomol. Chem.) Alan A. Wilson, Org. Biomol. Chem., 2010, DOI: 10.1039/b916419g To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 18, 2009 Category: Molecular Biology Authors: Alan A. WilsonArmando Garcia Sylvain Houle Neil Vasdev Source Type: journals

Imaging of Gadolinium Spatial Distribution in Tumor Tissue by Laser Ablation Inductively Coupled Plasma Mass SpectrometryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions We have demonstrated, in a novel manner, the use of LA–ICP–MS for the spatial detection of Gd in tumor tissue. LA–ICP–MS is valuable in providing spatio-specific information of MRI contrast agents and more importantly Gd in tumor tissue. Content Type Journal ArticleCategory Brief ArticleDOI 10.1007/s11307-009-0282-4Authors Nazila Kamaly, Hammersmith Hospital, Imperial College London Imaging Sciences Department, Metabolic and Molecular Imaging Group, MRC Clinical Sciences Center London W12 0NN UKJohn A. Pugh, University of Sheffield Department of Chemistry, Center for Analytical Scie...
Source: Molecular Imaging and Biology - November 17, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals

High Impact of 18F-FDG-PET on Management and Prognostic Stratification of Newly Diagnosed Small Cell Lung CancerEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion PET had a major impact on stage classification, management, and prognostic stratification of newly diagnosed SCLC. Content Type Journal ArticleCategory Research ArticleDOI 10.1007/s11307-009-0295-zAuthors Arun Azad, Ludwig Institute Oncology Unit, Austin Health Heidelberg AustraliaFiona Chionh, Ludwig Institute Oncology Unit, Austin Health Heidelberg AustraliaAndrew M. Scott, Centre for Positron Emission Tomography, Austin Health Melbourne AustraliaSzeting T. Lee, Centre for Positron Emission Tomography, Austin Health Melbourne AustraliaSam U. Berlangieri, Centre for Positron Emission Tomogra...
Source: Molecular Imaging and Biology - November 17, 2009 Category: Molecular Biology Tags: Molecular Imaging and Biology Source Type: journals

Fetal microchimerism: the cellular and immunological legacy of pregnancyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Review ArticlesDavid M. Lissauer, Karen P. Piper, Paul A.H. Moss, Mark D. Kilby, Expert Reviews in Molecular Medicine, Volume 11 , pp e33AbstractDuring pregnancy there is transplacental traffic of fetal cells into the maternal circulation. Remarkably, cells of fetal origin can then persist for decades in the mother and are detectable in the circulation and in a wide range of tissues. Maternal CD8 T cell responses directed against fetal antigens can also be detected following pregnancy. However, the impact that the persistence of allogenic cells of fetal origin and the maternal immune response towards them has on the mother...
Source: Expert Reviews in Molecular Medicine - November 17, 2009 Category: Molecular Biology Source Type: journals

BioEssays 12/2009Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cover Photograph: 3D computer graphics of DNA transcription in eukaryotes. Previously determined molecular structures were placed in this scene according to available experimental data and rendered using the Blender software (). Please see article by Wong, Winn and Mozziconacci . (Source: BioEssays)
Source: BioEssays - November 17, 2009 Category: Molecular Biology Authors: Hua Wong, Peter J Winn, Julien Mozziconacci Tags: Cover Picture Source Type: journals

Identification of an exonic splicing silencer in exon 6A of the human VEGF geneEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusion: This work establishes the identity of cis sequences that regulate alternative VEGFsplicing and dictate the relative expression levels of VEGF isoforms. (Source: BMC Molecular Biology - Latest articles)
Source: BMC Molecular Biology - Latest articles - November 17, 2009 Category: Molecular Biology Authors: Rui WangRonald CrystalNeil Hackett Source Type: journals

DASMiner: discovering and integrating data from DAS sourcesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: The support of the DAS protocol allows that hundreds of molecular biology databases to be treated as a federated, online collection of resources. DASMiner enables full exploration of these resources, and can be used to deploy applications and create integrated views of biological systems using the information deposited in DAS repositories. (Source: BMC Systems Biology - Latest articles)
Source: BMC Systems Biology - Latest articles - November 17, 2009 Category: Molecular Biology Authors: Diogo VeigaHelena DeusCaner AkdemirAna Tereza VasconcelosJonas Almeida Source Type: journals

1,1[prime or minute]-Binaphthyl-based imidazolium chemosensors for highly selective recognition of tryptophan in aqueous solutionsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Li Yang, Song Qin, Xiaoyu Su, Fei Yang, Jingsong You, Changwei Hu, Rugang Xie, Jingbo Lan (Paper from Org. Biomol. Chem.) Li Yang, Org. Biomol. Chem., 2010, DOI: 10.1039/b908540h To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 17, 2009 Category: Molecular Biology Authors: Li YangSong Qin Xiaoyu Su Fei Yang Jingsong You Changwei Hu Rugang Xie Jingbo Lan Source Type: journals

Abstracts from the Proceedings of the XXVII National Conference of CytometryEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
No abstract. (Source: Cytometry Part A)
Source: Cytometry Part A - November 17, 2009 Category: Molecular Biology Source Type: journals

Coming next month:Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
No Abstract. (Source: BioEssays)
Source: BioEssays - November 17, 2009 Category: Molecular Biology Tags: Forthcoming Articles Source Type: journals

Nature of methods in science: technology driven science versus science driven technologyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
No Abstract. (Source: BioEssays)
Source: BioEssays - November 17, 2009 Category: Molecular Biology Tags: Correspondence Source Type: journals