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Per-channel basis normalization methods for flow cytometry dataEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Between-sample variation in high-throughput flow cytometry data poses a significant challenge for analysis of large-scale data sets, such as those derived from multicenter clinical trials. It is often hard to match biologically relevant cell populations across samples because of technical variation in sample acquisition and instrumentation differences. Thus, normalization of data is a critical step before analysis, particularly in large-scale data sets from clinical trials, where group-specific differences may be subtle and patient-to-patient variation common. We have developed two normalization methods that remove technic...
Source: Cytometry Part A - November 7, 2009 Category: Molecular Biology Authors: Florian Hahne, Alireza Hadj Khodabakhshi, Ali Bashashati, Chao-Jen Wong, Randy D. Gascoyne, Andrew P. Weng, Vicky Seyfert-Margolis, Katarzyna Bourcier, Adam Asare, Thomas Lumley, Robert Gentleman, Ryan R. Brinkman Source Type: journals

Computational and Systems BiologyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
(Editorial from Mol. BioSyst.) Mol. BioSyst., 2009, DOI: 10.1039/b921381n To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Mol. BioSyst. latest articles)
Source: RSC - Mol. BioSyst. latest articles - November 6, 2009 Category: Molecular Biology Authors: RSC Source Type: journals

Flow immunocytochemistry of marker expression in cells from body cavity fluidsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Diagnostic cytology based on the examination of cells from body cavity fluids misses [sim]50% of patients with a proven malignancy. In an earlier study, we used immunohistochemical detection of epithelial membrane antigen expression with flow cytometric detection of DNA aneuploidy to reduce the number of false negatives. In the present study, we have combined DNA flow cytometry with flow cytometric detection of marker expression to analyze cells from body cavity fluids. Seventy-nine specimens of ascites and pleural fluids were analyzed by diagnostic cytology, DNA flow cytometry, and for the expression of the following mark...
Source: Cytometry Part A - November 6, 2009 Category: Molecular Biology Authors: Awtar Krishan, Parvin Ganjei-Azar, Ronald Hamelik, Deepti Sharma, Isildinha Reis, Mehrdad Nadji Source Type: journals

A quantitative measure for alterations in the actin cytoskeleton investigated with automated high-throughput microscopyEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The actin cytoskeleton modulates a large variety of physiological and disease-related processes in the cell. For example, actin has been shown to be a crucial host factor for successful infection by HIV-1, but the underlying mechanistic details are still unknown. Automated approaches open up the perspective to clarify such an issue by processing many samples in a high-throughput manner. To analyze the alterations in the actin cytoskeleton within an automated setting, large-scale image acquisition and analysis were established for JC-53 cells stained for actin. As a quantitative measure in such an automated approach, we sug...
Source: Cytometry Part A - November 6, 2009 Category: Molecular Biology Authors: Julian Weichsel, Nikolas Herold, Maik J. Lehmann, Hans-Georg Kräusslich, Ulrich S. Schwarz Source Type: journals

BH3-only proteins: The death-puppeteer's wiresEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Most cell death in vertebrates proceeds through the intrinsic pathway of apoptosis and results from unregulated increase of mitochondrial membrane permeability. Bcl2-associated X protein (Bax) and Bcl2-antagonist/killer protein (Bak), the effector proapoptotic members of the Bcl-2 family, are, in their active state, the principal accomplices for this permeabilization process. How exactly Bax and Bak are activated has been a matter of major investigation in the last decade, and suitable tools offered by quantitative cytometric methodologies have significantly contributed to the understanding of the function of Bcl-2 family ...
Source: Cytometry Part A - November 6, 2009 Category: Molecular Biology Authors: Fabio Ghiotto, Franco Fais, Silvia Bruno Source Type: journals

On the Emergence of Biochemical Homochirality: An Elusive Beginning.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19890734 [PubMed - as supplied by publisher] (Source: Origins of Life and Evolution of the Biosphere)
Source: Origins of Life and Evolution of the Biosphere - November 6, 2009 Category: Molecular Biology Authors: Pizzarello S, Lahav M Tags: Orig Life Evol Biosph Source Type: journals

Asymmetric ruthenium-catalyzed 1,4-additions of aryl thiols to enonesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Andrei Badoiu, Gerald Bernardinelli, Celine Besnard, E. Peter Kundig (Paper from Org. Biomol. Chem.) Andrei Badoiu, Org. Biomol. Chem., 2010, DOI: 10.1039/b918877k To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 6, 2009 Category: Molecular Biology Authors: Andrei BadoiuGerald Bernardinelli Celine Besnard E. Peter Kundig Source Type: journals

Host-guest complexations of local anaesthetics by cucurbit[7]uril in aqueous solutionEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ian W. Wyman, Donal H. Macartney (Paper from Org. Biomol. Chem.) Ian W. Wyman, Org. Biomol. Chem., 2010, DOI: 10.1039/b915694a To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 6, 2009 Category: Molecular Biology Authors: Ian W. WymanDonal H. Macartney Source Type: journals

Ghrelin in gastrointestinal diseases and disorders: a possible role in the pathophysiology and clinical implications (review).Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ghrelin is a peptide hormone, which has been isolated from the stomach. It is localized mostly in endocrine cells in the oxyntic mucosa of the stomach. Ghrelin receptors are expressed equally in all parts of the gastrointestinal tract, with a similar level of expression in the mucosal and muscle layers. This peptide hormone has several functions, the most widely known is its growth hormone (GH)-releasing effect. Ghrelin plays an important role in regulating appetite, feeding and energy metabolism. It also plays a role in mediating immune response and inflammatory processes. Ghrelin stimulates gastric motility and empty...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: El-Salhy M Tags: Int J Mol Med Source Type: journals

Diagnosis of parathyroid carcinoma using immunohistochemical staining against hTERT.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The differential diagnosis of parathyroid carcinoma from benign adenoma is often difficult when its typical clinicopathological features are absent, even with the aid of various molecular markers. We recently demonstrated that telomerase activation through hTERT expression is a unique characteristic that is limited to parathyroid carcinoma and not seen in benign tumors. In the present study, we investigated hTERT expression in parathyroid tumors using immunohistochemistry in an attempt to determine its clinical utility. There was no evidence of immunoreactivity in the 4 normal parathyroid glands and the 18 typical aden...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Osawa N, Onoda N, Kawajiri H, Tezuka K, Takashima T, Ishikawa T, Miyauchi A, Hirokawa M, Wakasa K, Hirakawa K Tags: Int J Mol Med Source Type: journals

Neuronal and BBB damage induced by sera from patients with secondary progressive multiple sclerosis.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
An important component of the pathogenic process of multiple sclerosis (MS) is the blood-brain barrier (BBB) damage. We recently set an in vitro model of BBB, based on a three-cell-type co-culture system, in which rat neurons and astrocytes synergistically induce brain capillary endothelial cells to form a monolayer with permeability properties resembling those of the physiological BBB. Herein we report that the serum from patients with secondary progressive multiple sclerosis (SPMS) has a damaging effect on isolated neurons. This finding suggests that neuronal damaging in MS could be a primary event and not only secon...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Proia P, Schiera G, Salemi G, Ragonese P, Savettieri G, Di Liegro I Tags: Int J Mol Med Source Type: journals

Molecular characterization and quantification using state of the art solid-state adiabatic TOBSY NMR in burn trauma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
We describe a novel solid-state nuclear magnetic resonance (NMR) method that maximizes the advantages of high-resolution magic-angle-spinning (HRMAS), relative conventional liquid-state NMR approaches, when applied to intact biopsies of skeletal muscle specimens collected from burn trauma patients. This novel method, termed optimized adiabatic TOtal through Bond correlation SpectroscopY (TOBSY) solid-state NMR pulse sequence for two-dimensional (2D) 1H-1H homonuclear scalar-coupling longitudinal isotropic mixing, was demonstrated to provide a 40-60% improvement in signal-to-noise ratio (SNR) relative to its liquid-state an...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Righi V, Andronesi O, Mintzopoulos D, Tzika AA Tags: Int J Mol Med Source Type: journals

Human platelet 12-lipoxygenase: naturally occurring Q261/R261 variants and N544L mutant show altered activity but unaffected substrate binding and membrane association behavior.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The single nucleotide polymorphism (SNP) R261Q in the human platelet 12-lipoxygenase has been correlated with several human diseases. To understand better the biological performance we have compared enzymatic properties of the recombinant enzymes: 'wild-type' as Q261 and R261 variants with a single Q261R mutation at the enzyme periphery and N544L mutant with an altered active site. The R261 variant does not follow the same kinetics such as WT-Q261 showing a lag phase, a slower accumulation of product, following a different time-course without reaching plateau characteristic for the Q261 variant. The N544L substitution ...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Aleem AM, Wells L, Jankun J, Walther M, Kühn H, Reinartz J, Skrzypczak-Jankun E Tags: Int J Mol Med Source Type: journals

Evaluation of annexin II as a potential serum marker for hepatocellular carcinoma using a developed sandwich ELISA method.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Annexin II (Annexin A2, ANXA2) is a 36 kDa calcium-dependent phospholipid-binding protein that is located on the surface of most eukaryotic cells. ANXA2 is involved in several biological processes, including anti-inflammatory effects, Ca27+-dependent exocytosis, immune responses, Ca2+ transport and phospholipase A2 regulation. In our previous study, ANXA2 was identified as an up-regulated gene in hepatocellular carcinoma (HCC) tissue by cDNA microarray. In the present study, we have evaluated ANXA2 as a tumor-associated marker of HCC. We determined the ANXA2 levels in human liver tissues with HCC using real-time RT-PCR...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Ji NY, Park MY, Kang YH, Lee CI, Kim DG, Yeom YI, Jang YJ, Myung PK, Kim JW, Lee HG, Kim JW, Lee K, Song EY Tags: Int J Mol Med Source Type: journals

Experimental study of the anti-cancer mechanism of tanshinone IIA against human breast cancer.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was performed to determine the anti-cancer activity of tanshinone IIA on human breast cancer cells in vitro and in vivo and to elucidate the underlying mechanism of this activity. Human breast cancer cell lines (estrogen receptor-positive and -negative) were treated with tanshinone IIA and tamoxifen. The inhibitory effects of tanshinone IIA and tamoxifen on breast cancer cell proliferation were examined using MTT assays, BrdU incorporation, immunohistochemistry and flow cytometry. Upon treatment with tanshinone IIA, breast cancer cell proliferation was significantly inhibited in a dose- and time-dependent manner...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Lu Q, Zhang P, Zhang X, Chen J Tags: Int J Mol Med Source Type: journals

Neuropeptide B (NPB) and neuropeptide W (NPW) system in cultured rat calvarial osteoblast-like (ROB) cells: NPW and NPB inhibit proliferative activity of ROB cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Neuropeptides B (NPB) and W (NPW) have been identified as endogenous ligands of two G-protein-coupled receptors, neuropeptides B/W receptor 1 (NPBWR1, formerly known as GPR7) and neuropeptides B/W receptor 2 (NPBWR2, formerly known as GPR8). In rodents where NPBWR2 is absent, its counterpart is named the similar to neuropeptides B/W receptor 2 (similar to NPBWR2, formerly GPR8-like). Both NPB and NPW play a role in the control of feeding, neuroendocrine axis functions, memory and learning processes as well as in pain regulation. The present study aimed to investigate the expression of NPB, NPW, NPBWR1 and the similar t...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Ziolkowska A, Rucinski M, Tyczewska M, Malendowicz LK Tags: Int J Mol Med Source Type: journals

REIC/Dkk-3 stable transfection reduces the malignant phenotype of mouse prostate cancer RM9 cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The reduced expression in immortalized cells (REIC)/Dickkopf (Dkk)-3, a member of the Dkk gene family, is a tumor suppressor in a broad range of cancers. REIC/Dkk-3 transfected stable clones of mouse prostate cancer RM9 cells (RM9-REIC) and the empty vector-transfected control clone cells (RM9-EV) were established. Clones were used to evaluate the anti-cancer effects and a proteomics analysis of REIC/Dkk-3 continuous expression was performed. The RM9-REIC cells show a feeble appearance and the cell membrane shows irregular buds known as blebs. In vitro cell proliferation was significantly suppressed in RM9-REIC clones ...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Chen J, Watanabe M, Huang P, Sakaguchi M, Ochiai K, Nasu Y, Ouchida M, Huh NH, Shimizu K, Kashiwakura Y, Kaku H, Kumon H Tags: Int J Mol Med Source Type: journals

Dysfunction of Ca2+/CaM kinase IIalpha cascades in the amygdala in post-traumatic stress disorder.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we investigated changes in Ca2+-CaM-CaMKIIalpha in the basolateral amygdala of rats after SPS which may reveal part of the pathogenesis of PTSD. The intracellular free calcium level in the basolateral amygdala was examined by fluorescence spectrophotometry. CaM and CaMKIIalpha expression in basolateral amygdala was examined using immunohistochemistry, Western blotting and reverse transcription-polymerase chain reaction. The intracellular free calcium level in the basolateral amygdala was increased when compared to that in the control group 1 day after SPS exposure (P<0.05). CaM expression significantly in...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Xiao B, Han F, Shi YX Tags: Int J Mol Med Source Type: journals

Lambda-carrageenan oligosaccharides elicit reactive oxygen species production resulting in mitochondrial-dependent apoptosis in human umbilical vein endothelial cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Previous studies have shown that highly sulfated lambda-carrageenan oligosaccharides (lambda-CO) possess an anti-angiogenetic effect, while high concentrations of lambda-CO present a cytotoxic effect towards human umbilical vein endothelial cells (HUVECs). The aim of this study was to explore the underlying mechanism of lambda-CO on inhibiting cell proliferation. lambda-CO elicited reactive oxygen species (ROS) production with concentrations at 0.8 and 1 mg/ml, and this event was accompanied by the increase of early apoptotic cells, nuclear morphology changes and cell cycle arrest at the S and G2/M phases. However, pre...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Chen HM, Yan XJ, Mai TY, Wang F, Xu WF Tags: Int J Mol Med Source Type: journals

Neurotrophic receptor tyrosine kinase B induces c-fos-associated cell survival.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we established 293T cells stably expressing human TrkB to elucidate its intracellular functions. Using this cell system, we examined the biological roles of TrkB and its downstream target molecules. The TrkB expressing cells showed an increased survival rate through increased c-fos mRNA expression by BDNF, which were completely suppressed by TrkB inhibitor. Moreover, the combination of inhibitors of mitogen-activated protein kinase (MEK) and phosphatidylinositol 3-kinase (PI3K) partially reduced both the cell survival rate and c-fos mRNA expression, whereas monotreatment of these reagents could not affect ce...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Hayashi A, Nakatani K, Nishioka J, Sakamoto Y, Jinda S, Wada H, Nobori T Tags: Int J Mol Med Source Type: journals

Coronary artery disease and depression: possible role of brain-derived neurotrophic factor and serotonin transporter gene polymorphisms.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cardiovascular disease (CVD) and depression are two of the most common human health problems. Patients with depression have an increased risk of developing cardiovascular disease and mortality after experiencing a cardiac event. Both diseases are complex disorders that are influenced by genetic and environmental factors. Brain-derived neuro-trophic factor (BDNF) plays a critical role in regulating both vascular development and response to injury, and promotes survival, differentiation, and maintenance of neurons in the peripheral and nervous system. Evidence suggests that BDNF can enhance serotoninergic transmission. S...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Bozzini S, Gambelli P, Boiocchi C, Schirinzi S, Falcone R, Buzzi P, Storti C, Falcone C Tags: Int J Mol Med Source Type: journals

Glycoprotein extraction from Laminaria japonica promotes IEC-6 cell proliferation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we isolated a novel glycoprotein from L. japonica that stimulates the growth of the IEC-6 normal murine intestinal epithelial cells. We also identified the mechanism by which this glycoprotein, referred to as LJGP, stimulates cell growth. After 24 h of exposure to LJGP, cell proliferation increased in a dose-dependent manner. To further explore the mechanism associated with LJGP-induced cell proliferation, we treated cells for various times with LJGP. We focused on the epidermal growth factor receptor (EGFR) signaling pathway, which is involved in the regulation of cellular proliferation and differentiation,...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Go H, Hwang HJ, Nam TJ Tags: Int J Mol Med Source Type: journals

Changes in the expression of cholesterol metabolism-associated genes in HCV-infected liver: a novel target for therapy?Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, we evaluated the expression of lipid metabolism-associated genes in patients with HCV infection by real-time PCR. Sterol regulatory element-binding protein (SREBP)-2 expression was unchanged and low density lipoprotein receptor expression was markedly reduced by 90% in HCV-infected liver. The expression of apolipoprotein B100, microsomal triglyceride transfer protein and ATP-binding cassette G5 was significantly increased. Up-regulation of cholesterol synthesis-associated genes, including HMG-CoA reductase, HMG-CoA synthase, farnesyl-diphosphate synthase and squalene synthase, confirmed enhanced de novo chol...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Nakamuta M, Yada R, Fujino T, Yada M, Higuchi N, Tanaka M, Miyazaki M, Kohjima M, Kato M, Yoshimoto T, Harada N, Taketomi A, Maehara Y, Koga M, Nishinakagawa T, Nakashima M, Kotoh K, Enjoji M Tags: Int J Mol Med Source Type: journals

Dislocation of Rab13 and vasodilator-stimulated phosphoprotein in inactive colon epithelium in patients with Crohn's disease.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Crohn's disease is associated with increased permeability of the intestine even in quiescent patients. Increased intestinal permeability may cause dysregulated immunological responses in the intestinal mucosa that leads to chronic intestinal inflammation. Tight junction proteins contribute to intestinal permeability, and functional abnormality and dislocation of such proteins may cause increased intestinal permeability. We studied the expression of tight junction proteins Rab13, vasodilator-stimulated phosphoprotein (VASP), zonula occludin-1 (ZO-1), and F-actin in the intestinal epithelium of patients with inactive inf...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Ohira M, Oshitani N, Hosomi S, Watanabe K, Yamagami H, Tominaga K, Watanabe T, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T Tags: Int J Mol Med Source Type: journals

Middle-term expansion of hematopoietic cord blood cells with new human stromal cell line feeder-layers and different cytokine cocktails.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cord blood (CB) is a source of hematopoietic stem cells (HSCs) and is an alternative to bone marrow for allogenic transplantation in patients with hematological disorders. The improvement of HSC in vitro expansion is one of the main challenges in cell therapy. Stromal components and soluble factors, such as cytokines, can be useful to induce in vitro cell expansion. Hence, we investigated whether feeder-layers from new stromal cell lines and different exogenous cytokine cocktails induce HSC expansion in middle-term cultures. CB HSC middle-term expansion was carried out in co-cultures on different feeder-layers exposed ...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: De Angeli S, Baiguera S, Del Pup L, Pavan E, Gajo GB, Di Liddo R, Conconi MT, Grandi C, Schiavon O, Parnigotto PP Tags: Int J Mol Med Source Type: journals

The effects of adiponectin on interleukin-6 and MCP-1 secretion in lipopolysaccharide-treated 3T3-L1 adipocytes: role of the NF-kappaB pathway.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
It was recently suggested that the transcription nuclear factor-kappaB (NF-kappaB) plays an important role in controlling the inflammation and metabolic alterations associated with obesity. In endothelial and monocytic cells, adiponectin acts as a modulator of the inflammatory response, suppressing NF-kappaB activation. The aim of this study was to assess the ability of different forms of adiponectin to modulate the inflammatory response in adipocytes. 3T3-L1 preadipocytes were cultured according to standard conditions. Fully differentiated adipocytes were stimulated with 1 microg/ml lipopolysaccharides (LPS) for 16 h,...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Zoico E, Garbin U, Olioso D, Mazzali G, Fratta Pasini AM, Di Francesco V, Sepe A, Cominacini L, Zamboni M Tags: Int J Mol Med Source Type: journals

Effects of protein kinase Cdelta and phospholipase C-gamma1 on monocyte chemoattractant protein-1 expression in taxol-induced breast cancer cell death.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monocyte chemoattractant protein-1 (MCP-1) is a CC chemokine that plays an important role in immune cell migration. It has been reported that chemokines, including MCP-1, are involved in angiogenesis and metastasis. However, the exact role of chemokines in cancer development is still obscure. We investigated the involvement of MCP-1 in taxol-induced breast cancer cell death. The anti-cancer drug taxol induced MCF-7 breast cancer cell death. Treatment with taxol increased the mRNA expression level of MCP-1 in a dose- and time-dependent manner. Up-regulation of MCP-1 by taxol was augmented in cells treated with rottlerin...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Kim YS, An HT, Kim J, Ko J Tags: Int J Mol Med Source Type: journals

The metabolic syndrome of omega3-depleted rats. IV. Intestinal phospholipid omega3 fatty acids.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
A dietary deprivation in long-chain polyunsaturated omega3 fatty acids initiated in 7-week old normal rats provokes within 3 to 7 months the appearance of several features of the metabolic syndrome. Likewise, within 2 to 4-5 weeks exposure to a flaxseed oil-enriched diet, these anomalies are rapidly corrected. The present study deals with the omega3 fatty acid content of intestinal phospholipids under the same experimental conditions. For the sake of comparison, the control rats were given access during the last 4-5 weeks to either a soybean or flaxseed oil-enriched diet. In control rats, the relative weight content of...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Hacquebard M, Portois L, Malaisse WJ, Carpentier YA Tags: Int J Mol Med Source Type: journals

The metabolic syndrome of omega3-depleted rats. V. Intestinal phospholipid omega6 fatty acids.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study aims mainly at investigating the effects of a dietary deprivation and replenishment of omega3 PUFA upon the phospholipid pattern of omega6 PUFA in the duodenum, jejunum, caecum and colon of rats exposed for 3-7 months to an omega3-depleted diet and then eventually exposed for 2-4 weeks to an omega3-rich diet. In control rats, the relative weight content of all omega6 fatty acids differed in the proximal and distal intestinal segments. In the omega3-depleted rats the C18:2omega6, C20:2omega6 and C20:3omega6 content was decreased whilst that of C20:4omega6 and C22:4omega6 was increased. Significant correlations we...
Source: International Journal of Molecular Medicine - November 5, 2009 Category: Molecular Biology Authors: Hacquebard M, Portois L, Malaisse WJ, Carpentier YA Tags: Int J Mol Med Source Type: journals

Using the ratio of means as the effect size measure in combining results of microarray experimentsEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Conclusions: Our results show that the proposed effect size measure (log ratio of means) has better power to identify differentially expressed genes, and that the detected genes have better performance in predicting cancer outcomes than the commonly used effect size measure, the standardized mean difference (SMD), under both quality-weighted and quality-unweighted data integration frameworks. The new effect size measure and the quality-weighted microarray data integration framework provide efficient ways to combine microarray results. (Source: BMC Systems Biology - Latest articles)
Source: BMC Systems Biology - Latest articles - November 5, 2009 Category: Molecular Biology Authors: Pingzhao HuCelia GreenwoodJoseph Beyene Source Type: journals

Glycosidase inhibition: assessing mimicry of the transition stateEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Tracey M. Gloster, Gideon J. Davies (Perspective from Org. Biomol. Chem.) Tracey M. Gloster, Org. Biomol. Chem., 2010, DOI: 10.1039/b915870g To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 5, 2009 Category: Molecular Biology Authors: Tracey M. GlosterGideon J. Davies Source Type: journals

A novel bimodal lipidic contrast agent for cellular labelling and tumour MRIEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Nazila Kamaly, Tammy Kalber, Gavin Kenny, Jimmy Bell, Michael Jorgensen, Andrew Miller (Paper from Org. Biomol. Chem.) Nazila Kamaly, Org. Biomol. Chem., 2010, DOI: 10.1039/b910561a To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 5, 2009 Category: Molecular Biology Authors: Nazila KamalyTammy Kalber Gavin Kenny Jimmy Bell Michael Jorgensen Andrew Miller Source Type: journals

Facile P,N-heterocycle synthesis via tandem aminomethylation-cyclization of H-phosphinate building blocksEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Clemence Queffelec, Jean-Luc Montchamp (Paper from Org. Biomol. Chem.) Clemence Queffelec, Org. Biomol. Chem., 2010, DOI: 10.1039/b917428a To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 5, 2009 Category: Molecular Biology Authors: Clemence QueffelecJean-Luc Montchamp Source Type: journals

Total synthesis of asperlicin C, circumdatin F, demethylbenzomalvin A, demethoxycircumdatin H, sclerotigenin, and other fused quinazolinonesEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Ming-Chung Tseng, Huei-Yun Yang, Yen-Ho Chu (Paper from Org. Biomol. Chem.) Ming-Chung Tseng, Org. Biomol. Chem., 2010, DOI: 10.1039/b910545j To cite this article before page numbers are assigned, use the DOI form of citation above. The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - Organic Biomolecular Chemistry)
Source: RSC - Organic Biomolecular Chemistry - November 5, 2009 Category: Molecular Biology Authors: Ming-Chung TsengHuei-Yun Yang Yen-Ho Chu Source Type: journals

Dual functions of Mdt1 in genome maintenance and cell integrity pathways in Saccharomyces cerevisiaeEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Recent evidence indicates considerable cross-talk between genome maintenance and cell integrity control pathways. The RNA recognition motif (RRM)- and SQ/TQ cluster domain (SCD)-containing protein Mdt1 is required for repair of 3[prime]-blocked DNA double-strand breaks (DSBs) and efficient recombinational maintenance of telomeres in budding yeast. Here we show that deletion of MDT1 (PIN4/YBL051C) leads to severe synthetic sickness in the absence of the genes for the central cell integrity MAP kinases Bck1 and Slt2/Mpk1. Consistent with a cell integrity function, mdt1[Delta] cells are hypersensitive to the cell wall toxin c...
Source: Yeast - November 5, 2009 Category: Molecular Biology Authors: Ana Traven, Tricia L. Lo, Brietta L. Pike, Helena Friesen, Julie Guzzo, Brenda Andrews, Jörg Heierhorst Source Type: journals

Normal table of postembryonic zebrafish development: Staging by externally visible anatomy of the living fishEmail this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The zebrafish is a premier model organism yet lacks a system for assigning postembryonic fish to developmental stages. To provide such a staging series, we describe postembryonic changes in several traits that are visible under brightfield illumination or through vital staining and epiflourescent illumination. These include the swim bladder, median and pelvic fins, pigment pattern, scale formation, larval fin fold, and skeleton. We further identify milestones for placing postembryonic fish into discrete stages. We relate these milestones to changes in size and age and show that size is a better indicator of developmental p...
Source: Developmental Dynamics - November 5, 2009 Category: Molecular Biology Authors: David M. Parichy, Michael R. Elizondo, Margaret G. Mills, Tiffany N. Gordon, Raymond E. Engeszer Source Type: journals

The role of mass spectrometry-based metabolomics in medical countermeasures against radiation.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Radiation metabolomics can be defined as the global profiling of biological fluids to uncover latent, endogenous small molecules whose concentrations change in a dose-response manner following exposure to ionizing radiation. In response to the potential threat of nuclear or radiological terrorism, the Center for High-Throughput Minimally Invasive Radiation Biodosimetry was established to develop field-deployable biodosimeters based, in part, on rapid analysis by mass spectrometry of readily and easily obtainable biofluids. In this review, we briefly summarize radiation biology and key events related to actual and poten...
Source: Mass Spectrometry Reviews - November 4, 2009 Category: Molecular Biology Authors: Patterson AD, Lanz C, Gonzalez FJ, Idle JR Tags: Mass Spectrom Rev Source Type: journals

The study of ribonucleoproteomics with mass spectrometry (MS)-based technology.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
PMID: 19890945 [PubMed - as supplied by publisher] (Source: Mass Spectrometry Reviews)
Source: Mass Spectrometry Reviews - November 4, 2009 Category: Molecular Biology Authors: Takahashi N Tags: Mass Spectrom Rev Source Type: journals

Controlled band dispersion for quantitative binding determination and analysis with electrospray ionization-mass spectrometry.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This review discusses recent emerging techniques that have been used to couple flow-injection analysis (FIA) and electrospray ionization-mass spectrometry (ESI-MS) for the quantitation of noncovalent binding interactions. Focus is placed predominantly on two such methods. Diffusion-based measurements, developed by Konermann and co-workers, uses controlled-band dispersion prior to ESI-MS to determine diffusion constants and binding constants based on the temporal variation of ligand signal measured in the mass spectrum (an indirect technique). Dynamic titration, developed by Schug and co-workers, is a direct method, whe...
Source: Mass Spectrometry Reviews - November 4, 2009 Category: Molecular Biology Authors: Schug KA, Serrano C, Fryčák P Tags: Mass Spectrom Rev Source Type: journals

Mass spectrometry of the photolysis of sulfonylurea herbicides in prairie waters.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This review of mass spectrometry of sulfonylurea herbicides includes a focus on studies relevant to Canadian Prairie waters. Emphasis is given to data gaps in the literature for the rates of photolysis of selected sulfonylurea herbicides in different water matrices. Specifically, results are evaluated for positive ion electrospray tandem mass spectrometry with liquid chromatography separation for the study of the photolysis of chlorsulfuron, tribenuron-methyl, thifensulfuron-methyl, metsulfuron-methyl, and ethametsulfuron-methyl. LC-MS/MS is shown to be the method of choice for the quantification of sulfonylurea herbic...
Source: Mass Spectrometry Reviews - November 4, 2009 Category: Molecular Biology Authors: Headley JV, Du JL, Peru KM, McMartin DW Tags: Mass Spectrom Rev Source Type: journals

Effect of the membrane permeable cAMP analog, dibutyryl cAMP, on caspase-dependent apoptosis, inflammatory gene expression, and graft survival in acute cardiac allograft rejection.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
This study was designed to investigate the effects of cAMP on immune regulation and apoptosis during acute rat cardiac allograft rejection. We found that the production of immune markers such as inflammatory cytokines (interlukin-1b, interlukin-6, and tumor necrosis factor-a), inducible nitric oxide synthase (iNOS) expression, and nitric oxide (NO) production, was significantly increased in the blood and transplanted hearts of allograft recipients, but not of isograft controls. These increases were effectively suppressed by the administration of the membrane permeable cAMP analog dibutyryl cAMP (db-cAMP). Administration of...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Lee JY, Kim JH, Chae G, Ha KS, Kwon YG, Kim YM Tags: Exp Mol Med Source Type: journals

Further understanding of fat biology: Lessons from a fat fly.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Obesity is a leading risk factor for insulin resistance, hypertension, hyperlipidemia, and cardiovascular complications, collectively referred to as metabolic diseases. Given the prevalence of obesity and its associated medical problems, new strategies are required to prevent or treat obesity and obesity-related metabolic effects. Here we summarize contributors of obesity, and molecular mechanisms controlling adipogenesis from studies in mammalian systems. We also discuss the possibilities of using Drosophila as a genetic model system to advance our understanding of players in fat biology. PMID: 19887892 [PubMed - ...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Hong JW, Park KW Tags: Exp Mol Med Source Type: journals

PTH accelerates decompensation following left ventricular hypertrophy.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
In this study, pressure overload-induced left ventricular hypertrophy (LVH) was induced in mice by transverse aortic banding (TAB) for 2 weeks. We subsequently evaluated the effects of a 2-week treatment with PTH or saline on compensated LVH. After another 4 weeks, the hearts of the mice were analyzed by echocardiography, histology, and molecular biology. Echocardiography showed that hearts of the PTH-treated mice have more severe failing phenotypes than the saline-treated mice following TAB with a greater reduction in fractional shortening and left ventricular posterior wall thickness and with a greater increase in left v...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Cha H, Jeong HJ, Jang SP, Kim JY, Yang DK, Oh JG, Park WJ Tags: Exp Mol Med Source Type: journals

Aspirin attenuates the anti-inflammatory effects of theophylline via inhibition of cAMP production in mice with non-eosinophilic asthma.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Theophylline is commonly used to treat severe asthma and chronic obstructive pulmonary disease (COPD) characterized by non-eosinophilic inflammation. Acetyl salicylic acid (ASA) is one of the most widely used medications worldwide, but up to 20% of patients with asthma experience aggravated respiratory symptoms after taking ASA. Here we evaluated the adverse effect of ASA on the therapeutic effect of theophylline in mice with non-eosinophilic asthma. A non-eosinophilic asthma mouse model was induced by airway sensitization with lipopolysaccharide (LPS)-containing allergen and then challenged with allergen alone. Therap...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Moon HG, Kim YS, Choi JP, Choi DS, Yoon CM, Jeon SG, Gho YS, Kim YK Tags: Exp Mol Med Source Type: journals

Induction of TIMP-2 by cholesterol depletion leads to the conversion of pro MMP-2 into active-MMP-2 in human dermal fibroblasts.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Cholesterol is one of major components of cell membrane and plays a role in vesicular trafficking and cellular signaling. We investigated the effects of cholesterol on MMP-2 activation in human dermal fibroblasts. We found that TIMP-2 expression and active form MMP-2 (64 kD) were dose-dependently increased by methyl-b-cyclodextrin (MbCD), a cholesterol depletion agent. In contrast, cholesterol depletion-induced TIMP-2 expression and MMP-2 activation were suppressed by cholesterol repletion. Then we investigated the regulatory mechanism of TIMP-2 expression by cholesterol depletion. We found that the phosphorylation of ...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Kim S, Oh JH, Lee Y, Lee J, Cho KH, Chung JH Tags: Exp Mol Med Source Type: journals

Neuronal RNA granule contains ApCPEB1, a novel Aplysia CPEB, in Aplysia sensory neuron.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
The cytoplasmic polyadenylation element (CPE)-binding protein (CPEB) binds to CPE containing mRNAs on their 3' untranslated regions (3'UTRs). This RNA binding protein comes out many important tasks, especially in learning and memory, by modifying the translational efficiency of target mRNAs via poly (A) tailing. Overexpressed CPEB has been reported to induce the formation of stress granules (SGs), a sort of RNA granule in mammalian cell lines. In these days, RNA granule is considered to be a potentially important factor in learning and memory. However, there is no study about RNA granule in Aplysia. To examine whether ...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Chae YS, Lee SH, Cheang YH, Lee N, Rim YS, Jang DJ, Kaang BK Tags: Exp Mol Med Source Type: journals

Orphan nuclear receptor small heterodimer partner inhibits angiotensin II- stimulated PAI-1 expression in vascular smooth muscle cells.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Angiotensin II is a major effector molecule in the development of cardiovascular disease. In vascular smooth muscle cells (VSMCs), angiotensin II promotes cellular proliferation and extracellular matrix accumulation through the upregulation of plasminogen activator inhibitor-1 (PAI-1) expression. Previously, we demonstrated that small heterodimer partner (SHP) represses PAI-1 expression in the liver through the inhibition of Transforming growth factor-b (TGF-b) signaling pathways. Here, we investigated whether SHP inhibited angiotensin II-stimulated PAI-1 expression in VSMCs. Adenovirus-mediated overexpression of SHP (...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Lee KM, Seo HY, Kim MK, Min AK, Ryu SY, Kim YN, Park YJ, Choi HS, Lee KU, Park WJ, Park KG, Lee IK Tags: Exp Mol Med Source Type: journals

Mass spectrometry based cellular phosphoinositides profiling and phospholipid analysis: a brief review.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Phospholipids are key components of cellular membrane and signaling. Among cellular phospholipids, phosphoinositides, phosphorylated derivatives of phosphatidylinositol are important as a participant in essential metabolic processes in animals. However, due to its low abundance in cells and tissues, it is difficult to identify the composition of phosphoinositides. Recent advances in mass spectrometric techniques, combined with established separation methods, have allowed the rapid and sensitive detection and quantification of a variety of lipid species including phosphoinositides. In this mini review, we briefly introd...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Kim Y, Shanta SR, Zhou LH, Kim KP Tags: Exp Mol Med Source Type: journals

The function of p27(KIP1) during tumor development.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Timely cell cycle regulation is conducted by sequential activation of a family of serine-threonine kinases called cycle dependent kinases (CDKs). Tight CDK regulation involves cyclin dependent kinase inhibitors (CKIs) which ensure the correct timing of CDK activation in different phases of the cell cycle. One CKI of importance is p27(KIP1). The regulation and cellular localization of p27(KIP1) can result in biologically contradicting roles when found in the nucleus or cytoplasm of both normal and tumor cells. The p27(KIP1) protein is mainly regulated by proteasomal degradation and its downregulation is often correlated...
Source: exp Mol Med - November 4, 2009 Category: Molecular Biology Authors: Lee J, Kim SS Tags: Exp Mol Med Source Type: journals

Age-dependent Preferential Dense-core Vesicle Exocytosis in Neuroendocrine Cells Revealed by Newly Developed Monomeric Fluorescent Timer Protein.Email this article to a colleague. Save this article to My Clippings. Discuss or comment on this article.
Monitoring Editor: Akihiko Nakano Although it is evident that only a few secretory vesicles accumulating in neuroendocrine cells are qualified to fuse with the plasma membrane and release their contents to the extracellular space, the molecular mechanisms that regulate their exocytosis are poorly understood. For example, it has been controversial whether secretory vesicles are exocytosed randomly or preferentially according to their age. Using a newly developed protein-based fluorescent timer, mK-GO, which changes color with a predictable time course, here we show that small GTPase Rab27A effectors regulate age-depende...
Source: Mol Biol Cell - November 4, 2009 Category: Molecular Biology Authors: Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A Tags: Mol Biol Cell Source Type: journals